The most important clinical signs that characterize keratoconus (KC) are the irregular-marked astigmatism with progressive apical protrusion due to progressive thinning of the corneal stroma [
Many authors prefer to classify the severity of keratoconus according to the mean keratometry readings. The Amsler-Krumeich classification graded keratoconus into 4 grades depending on the main K readings. Grade 1 included mean central K readings < 48 diopters, grade 2 included mean central K readings ≥ 48–< 54 diopters, grade 3 included mean central K readings ≥ 54–< 55 diopters while grade 4 included mean central K readings ≥ 55 diopters [
One of the most trustable treatment modalities in keratoconus management is the implantation of intracorneal rings (ICRS). Actually, ICRS gain more ground and popularity every day. The long-term stability results of ICRS together with the use of the femtosecond laser devices actually encouraged surgeons to implant ICRS simply and safely. Furthermore, they had a good impact on the patient satisfaction after surgery [
ICRS are one of the most commonly used rings all over the world. The use of one or two segments in segmented ICRS or complete rings depends on the type of the keratoconus cone, corneal topography patterns, specially designed nomograms, and the surgeons’ experiences. The main idea of ICRS implantation is to flatten the cornea and reduce anterior corneal surface irregularity, but they actually have no role in halting the progression of keratoconus [
Corneal collagen cross-linking (CXL) is the actual and main treatment to keratoconus and has the advantage of halting the progression of the pathology of the disease. The progression of keratoconus can be defined by continues change in 2 or more of special parameters. These special parameters included steepening of the posterior K readings, steepening of the anterior K readings, and thinning of the central pachymetry readings and high back surface elevations [
Recently, CXL PLUS has become more popular among surgeons as it has the advantages of both halting KC progression and improving the visual outcome. CXL is the main mandatory procedure that stabilizes the cornea and halts the disease progression, hence the name CXL PLUS as plus means adjuvant refractive procedure to improve vision which could be ICRS implantation, topography-guided PRK, toric implantable collamer lens (TICL), phakic intraocular lens (IOL), or even refractive lens surgery (clear lens extraction) with toric IOL implantation. Patient selection for the suitable refractive procedure is essential as not all previously mentioned refractive procedures are suitable for all patients as every keratoconic eye has its suitable refractive procedure. CXL PLUS is the most beneficial modality of treatment as the use of the combination therapy depends on many factors mainly the degree of myopia and astigmatism and the corneal pachymetry and keratometry readings [
This study was designed as a retrospective multicenter study that aimed to analyze the three-year outcomes of CXL PLUS (combined CXL and Keraring implantation with the use of femtosecond laser) concerning its efficacy and safety.
This study was a multicenter study that was carried on 3 universities including Sohag University Hospital, Banha University Hospital, and Minia University Hospital in Egypt. This study had the approval of the ethical committee in Sohag University Hospital.
Only eyes that completed 3-years of follow-up are included in this study as all eyes were performed and followed up during the period from January 2014 to May 2017. All follow-up results of the medical records were obtained retrospectively for analysis of the patients’ data.
43 eyes of 38 patients diagnosed with keratoconus were operated with CXL PLUS that included simultaneous combination of accelerated epithelium-on CXL and femtosecond laser Keraring implantation at the same session.
The 43 eyes were subjected to preoperative and postoperative UCVA, BCVA, manifest refraction, and Pentacam pachymetry and keratometry at 3-, 6-, 12-, 24-, and 36-month follow-up period.
The devices that were used in this study included CSO SIRIUS Topographer (CSO, Firenze, Italy), Advanced Femtosecond Laser (iFS; Abbott, USA), and the KXL System (Avedro Inc., MA, USA).
CXL PLUS included combined femtosecond laser Keraring (Mediphacos Inc., Belo Horizonte, Brazil) implantation as the first step in surgery followed immediately by accelerated epithelium-on CXL as the second step in the same session. The data that was introduced into the device included the parameters of the corneal tunnel creation with an outer diameter of 5.9 mm and an inner diameter of 5 mm; the incision site was at the steepest corneal meridian and the tunnel was created at the depth of 75% of the corneal thickness at the thinnest location.
The specification of the Keraring used in this study was the SI-5 design (5.0 mm optical zone) with triangular design in cross-section, made of polymethyl methacrylate (PMMA). The use of one or two Keraring segments depended upon the Keraring nomogram. Different parameters affected the choice of one or two segmented rings mainly the type and the site of the keratoconus cone, the mean K readings, the central pachymetry readings, the corneal thickness at the thinnest location, and the refractive status of the eye. This study included only a sample of keratoconic eyes that completed the 3-year follow-up period and were treated relying upon the designed Keraring nomogram. Although all eyes included in this study were treated with an implantation of 2 Keraring segments, yet it should be clear that this was not the concept to be generalized as a rule for all keratoconic eyes. Moreover, the characteristics of these eyes included in this study required implantation of 2 Keraring segments in each eye using the Mediphacos Nomogram for Keraring calculation guidelines 2009 version 5.2. Figure
CXL PLUS in the right eye; (a) marking the corneal center when the patient is fixating at flashing light, (b) suction ring application onto the cornea, (c) corneal tunnel creation using the femtosecond laser, (d) checking the patency of the tunnel by passing a spatula through the limbs of the tunnel, (e) implantation of the temporal Keraring segment, (f) implantation of the nasal Keraring segment, (g) riboflavin dropping onto the cornea, and (h) accelerated epithelium-on CXL.
The first step started with marking the corneal center when the patient is fixating at the flashing light (Figure
The second step was to perform the epithelium-on CXL using Avedro Nomogram with Avedro KXL CXL system. Accelerated epithelium-on CXL included installation of riboflavin (ParaCel) on the cornea every 90 seconds which is repeated 3 times along duration of four and half minutes. The next step was to install another type of riboflavin (Vibex Xtra) that was also performed every 90 seconds which is repeated 4 times along a duration of six minutes. The total soaking time was 11 minutes. Accelerated epithelium-on CXL was performed by using the pulsed UV mode (1 second on 1 second off) with UV time 2 minutes and 40 seconds while the total treatment time was 5 minutes and 20 seconds at a power of 45 mW/cm2 to deliver a total energy of 7.2 J/cm2. Application of bandage contact lens onto the cornea and installation of antibiotic eye drops (moxifloxacin 0.5%) into the eyes were performed at the end of the surgery. Dropping of riboflavin onto the cornea (Figure
All patients received the same postoperative treatment which lasted for three weeks. The postoperative regimen included topical therapy in the form of corticosteroid eye drops (prednisolone acetate 1%) in addition to antibiotic eye drops (moxifloxacin 0.5%) and artificial tears (hypotonic sodium hyaluronate 0.15%). All eye drops were prescribed for all patients on 2 hourly bases on the first postoperative day then 4 times daily in the first week. The frequency of medication was tapered to be 3 times daily in the second week and twice daily in the third week. Bandage contact lens was removed at the first postoperative day.
Analysis of the data was performed using the statistical package for social sciences software (SPSS version 22 for Windows). The description of the quantitative data was by using the median, mean and standard deviation. The presentation of the qualitative data was in the form of number and percentage. Paired sample
This study included 43 eyes of 38 patients. 23 patients were females (60.5%) while 15 patients were males (39.5%). All patients fall in the age group from 14 to 25 years with a mean age of 19.58 ± 4.05 (mean ± SD). Table
Characteristics of the study patients.
Item | Number | Percent |
---|---|---|
Total number of study eyes | 43 | |
Total number of study patients | 38 | |
Age | ||
Range | 14–25 years | |
Mean ± SD | 19.58 ± 4.05 | |
Gender | ||
Male | 15 patients | 39.5% |
Female | 23 patients | 60.5% |
Laterality | ||
OD | 18 eyes | 41.9% |
OS | 25 eyes | 58.1% |
Two segments of Kerarings were implanted in all 43 keratoconic eyes in this study. Keraring model SI-5 (5 mm optical zone) segments were used in this study.
All preoperative and postoperative data collected from the patient’s files over a follow-up period of 36 months were summarized in Table
CXL PLUS preoperative and postoperative data summary at the end of the 3-year follow-up.
Parameters | Preoperative data |
Postoperative 36th month data (mean ± SD) |
|
---|---|---|---|
Mean K reading average (D) | 50.63 ± 0.87 | 45.56 ± 0.98 | <0.05 |
Mean K1 reading | 46.94 ± 0.26 | 46.45 ± 0.98 | <0.05 |
Mean K2 reading | 54.38 ± 1.17 | 47.39 ± 0.62 | <0.05 |
Mean corneal thickness at thinnest location (μm) | 471 ± 92.36 | 423 ± 39.85 | <0.05 |
Mean postoperative myopic correction (D) | — | 0.97 ± 0.28 | |
Mean astigmatism (D) | 7.55 ± 1.75 | 3.39 ± 1.26 | <0.05 |
Mean postoperative astigmatic correction (D) | — | 3.8 ± 1.67 | |
Mean UCVA (logMAR) | 1.30 ± 0.48 | 0.82 ± 0.22 | <0.05 |
Mean BCVA (logMAR) | 0.90 ± 0.40 | 0.60 ± 0.30 | <0.05 |
This study showed the great influence of the CXL PLUS in reducing the mean K average that was reduced from 50.63 ± 0.87 diopters (mean ± SD) preoperatively to 45.56 ± 0.98 postoperatively (
Furthermore, these 5 diopters reduction in the mean postoperative K average resulted from the combination of correction in both mean postoperative myopic and astigmatic correction. As the results in this study indicated the correction of postoperative myopic component up to 1 D approximately while the marked correction was shown in the postoperative astigmatic component up to a level of 4 D approximately.
These good results were reflected on the patient both UCVA and BCVA postoperatively. This study showed improvement of the preoperative mean UCVA from 1.30 ± 0.48 (logMAR ± SD) to a postoperative mean UCVA 0.82 ± 0.22 (
This study also showed the reduction of the preoperative mean corneal thickness at the thinnest location 471 ± 92.36
Table
A comparison between the preoperative and postoperative data of one female patient after 3 years of CXL PLUS.
Variant | Preoperative | Postoperative (36th month) | ||||
---|---|---|---|---|---|---|
Corneal topography (tangential anterior) |
|
|
||||
K average (D) | 50.39 | 47.075 | ||||
K readings (D) | 47.52 | 53.26 | 46.08 | 48.07 | ||
Subjective refraction | −2.57 | −7.00 @ 96 | −0.50 | −0.75 @ 20 | ||
Corneal thickness at the thinnest location (μm) | 402 | 387 | ||||
Myopic component (D) | 2.75 | 2.00 | ||||
Astigmatic component (D) | 5.74 | 1.00 | ||||
Myopic correction | — | 0.75 | ||||
Astigmatic correction | — | 4.74 | ||||
UCVA (logMAR) | 1.30 | 0.40 | ||||
BCVA (logMAR) | 0.52 | 0.22 |
Complications were recorded in 7 eyes (16.3%) in this study. One eye was complicated by exposure of the edge of the Keraring segment within the first postoperative month so that the ring was removed and the procedure was repeated 3 months later with success.
Another complication that was recorded in this study was the progression of keratoconus and deterioration of the condition in 6 eyes so that the cross-linking procedure was repeated in all 6 eyes using the standard conventional 30 minutes epithelium-off CXL.
Six eyes (14% of eyes) showed progression of keratoconus, the CXL was repeated in these 6 eyes using the standard conventional 30 minutes CXL procedure (The Dresden Protocol), and the cornea was exposed to UV irradiation using Opto XLink-Corneal Crosslinking System (Opto Global Pty Ltd., Adelaide, Australia). The UV irradiation was performed at a power of 1.50 mW, an intensity of 2.984 mW/cm3, and a dose of 5.371 J/cm3 for 30 minutes. 8 mm deepithelized. corneal zone was performed for all 6 eyes followed by dropping of riboflavin VibeX Rapid™ (Avedro Inc., MA, USA) every 3 minutes for 30 minutes then corneal irradiation with UVA for 30 minutes with continuation of riboflavin dropping during corneal irradiation.
The eyes were followed during the period of the study to complete the 3-year follow-up and surprisingly corneal flattening occurred with improvement of the K readings and further thinning of the corneal thickness. So that the accelerated epithelium-on CXL was effective in 37 eyes to halt the progression of keratoconus (86% of eyes) while it failed to stop the deterioration of 6 keratoconic eyes (14% of eyes) that was treated with standard conventional 30 minutes epithelium-off CXL.
According to the postoperative complications, the 43 keratoconic eyes included in this study were divided into 3 postoperative groups according to the results:
Group A included 36 eyes (83.7% of eyes) that were subjected to CXL PLUS using simultaneous epithelium-on CXL and 2 Keraring segments implantation and showed good improvement with stability of the results along the 3-year follow-up period.
Group B included 6 eyes (14% of eyes) that were subjected to CXL PLUS using simultaneous epithelium-on CXL and 2 Keraring segments implantation but showed further deterioration within the first 3 months postoperatively so that these 6 eyes were subjected to additional conventional standard epithelium-off CXL procedure which managed to stop further KC deterioration and showed good stability and further improvement along the 3-year follow-up period. Table
The summary of the refractive data of the six complicated eyes (group B).
Parameters | Preoperative data (mean ± SD) | 3 months following CXL PLUS (mean ± SD) | 36 months following epithelium-off CXL (mean ± SD) |
---|---|---|---|
Mean K reading average (D) | 49.52 ± 0.93 | 50.18 ± 0.59 | 48.06 ± 0.42 |
Mean corneal thickness at thinnest location, ( |
453 ± 37.81 | 429 ± 31.05 | 399 ± 53.89 |
Mean postoperative myopic correction (D) | — | — | 1.02 ± 0.47 |
Mean astigmatism (D) | 5.96 ± 1.58 | 6.46 ± 0.87 | 4.62 ± 1.14 |
Mean postoperative astigmatic correction (D) | — | — | 1.76 ± 0.25 |
Mean UCVA (logMAR) | 1.12 ± 0.60 | 1.30 ± 0.52 | 0.90 ± 0.28 |
Mean BCVA (logMAR) | 0.82 ± 0.30 | 1.00 ± 0.40 | 0.70 ± 0.25 |
Group C included 1 eyes (2.3%) was complicated by Keraring exposure during the first postoperative month. The exposed segment was removed together with the second segment in the same session. Three months later, another 2 Keraring segments were implanted. The eye was followed up for 3 years and showed good improvement and stability of the condition. Table
The summary of the refractive data of the 7th complicated eye (group C).
Parameters | Preoperative data | Following removal of the 2 Keraring segments | 36 months following reimplantation of 2 new Keraring segments |
---|---|---|---|
Mean K reading average (D) | 51.37 | 52.04 | 48.06 |
Mean corneal thickness at thinnest location, ( |
428 | 426 | 421 |
Mean postoperative myopic correction (D) | — | — | 1.25 |
Mean astigmatism (D) | 5.75 | 5.50 | 1.50 |
Mean postoperative astigmatic correction (D) | — | — | 1.00 |
Mean UCVA (logMAR) | 1.30 | 1.30 | 0.70 |
Mean BCVA (logMAR) | 0.60 | 0.60 | 0.30 |
Table
The summary of complications.
Complication | Number of eyes | Percent | Management of the complications | Follow-up results along the 36 months |
---|---|---|---|---|
Postoperative KC progression | 6 | 14% | Standard 30 minutes epithelium-off CXL | Improvement with halting KC progression |
Exposure of the Keraring edge | 1 | 2.3% | Explanation of the 2 Keraring segments followed by reimplantation of the Keraring segments 3 months later | Improvement with stability of the segments |
The reliability of the results of this study arises from the long-term follow-up period (3 years).
CXL PLUS was proved to be effective and relatively safe in this study. The use of Kerarings for mechanical corneal flattening was effective in reducing the postoperative K readings.
Analysis of the preoperative and postoperative data of the patients at the 36th postoperative follow-up months was performed. The results of this analysis together with collecting the missing tiny points helped this study to make a clear impression upon the CXL PLUS. This study showed clearly that Keraring was very effective in flattening the steep corneal meridians thus reducing markedly the astigmatic component of KC and help correcting the anterior corneal surface irregularities. Furthermore, the use of Kerarings improved the postoperative astigmatism up to 5 D or even more in few cases. The procedure of the accelerated epithelium-on was originally introduced to stop deterioration of the disease and was not expected to improve vision or even flattening the cornea.
The discrepancy between the two CXL procedures (epithelium-on and epithelium-off) showed the importance of reevaluating the two procedures as it seemed that the conventional 30 minutes epithelium-off CXL was more effective in halting the KC progression and flattening of the corneal surface thus sharing in correcting the myopic component of KC and reducing the anterior irregularities. On the contrary, the accelerated epithelium-on CXL was relatively effective in halting the KC progression in most cases while in some cases it was not actually effective to stop the disease progression.
Many authors had proved in their studies the effectiveness of Keraring implantation regarding better visual acuity and decreasing the spherical equivalent and keratometry readings. One of them was Gharaibeh et al. [
This study did not include the use of Keraring 355° meanwhile many other studies reported its use in treating KC. Jadidi et al. [
One of the most interesting studies were Kubaloglu et al. [
In Alexandria, Ibrahim and colleagues [
Shawky et al. [
Other authors reported the results of CXL PLUS using different techniques by combining CXL and topography-guided photorefractive keratectomy (PRK) whether in the same session or sequential. Bor’i [
Moreover, El-Raggal [
In another comparative study, Iqbal [
Furthermore, in a different comparative study to the same author, Iqbal [
Moreover, many authors proved in their studies that epithelium-off CXL was more effective that transepithelial CXL. Kocaka [
CXL PLUS proved to be a successful procedure to halt progression (mainly by CXL) and to correct the refractive status of the keratoconic eye (mainly by ICRS). CXL PLUS performed a synergistic action correcting and maintaining the correction of both myopic and astigmatic components of keratoconus. This study proved that Keraring implantation is more effective in lowering the astigmatic component of KC than the myopic component. This study recorded KC progression in 14% of cases following CXL PLUS with the accelerated epithelium-on CXL so that it is recommended to perform new prospective comparative studies in the future comparing both epithelium-on CXL and epithelium-off CXL procedures in CXL PLUS for the treatment of keratoconus.
The authors declare that they have no conflicts of interest.
The authors acknowledge the sincere help and support of Dr. Amr Mounir, Dr. Islam El Saman, Dr. Islam Awny, Engineer Tamer Badawy, Engineer Ahmed Saber, and Mister El Khominy for their great effort and all facilities they have provided for the authors.