Journal of Ophthalmology
Volume 2019 (2019), Article ID 9721085, 5 pages
https://doi.org/10.1155/2019/9721085

Research Article

Measurement of the Orbital Soft Tissue Volume in Chinese Adults Based on Three-Dimensional CT Reconstruction

Yi Du, Bing-Yao Lu, Jun Chen, and Jian-Feng He

Department of Ophthalmology, The First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning 530021, Guangxi, China

Correspondence should be addressed to Yi Du; duyi@gxmu.edu.cn

Received 2 April 2019; Accepted 14 May 2019; Published 2 July 2019

Academic Editor: Sentaro Kusuhara

Copyright © 2019 Yi Du et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Quantitative measurement of the orbital soft tissue volume plays a very important role in the study of orbital diseases. The purpose of this study is to establish a computed tomography- (CT-) based three-dimensional (3D) reconstruction model and measure the orbital soft tissue volume in Chinese adults. We collected data from 103 Chinese adults (52 males and 51 females) who underwent orbital CT. The CT images of these adults were used to reconstruct a 3D model of the orbital bony cavity, orbital fat, extraocular muscle, and intraorbital optic nerve using Mimics software, and their respective volumes were measured. The mean (±SD) orbital bony cavity volume (OV), orbital fat volume (FV), extraocular muscle volume (MV), and intraorbital optic nerve volume (iONV) of the males were 22.2 ± 2.2 cm³, 8.9 ± 1.8 cm³, 1.9 ± 0.34 cm³, and 0.41 ± 0.08 cm³, respectively. The mean OV, FV, MV, and iONV of the females were 20.2 ± 1.5 cm³, 8.1 ± 1.7 cm³, 1.6 ± 0.3 cm³, and 0.36 ± 0.074 cm³, respectively, which were all significantly lower than those in males (all ). FV (r = 0.370; ) and MV (r = 0.283; ) were significantly correlated with body mass index (BMI), while iONV was not correlated with BMI (r = −0.070; ). This study shows that FV, MV, and iONV were higher in males than in females. With increasing BMI, FV and MV both increased, but iONV did not exhibit this trend.

1. Introduction

Many ocular diseases can cause volume changes in orbital soft tissues; e.g., thyroid-associated ophthalmopathy can lead to extraocular muscle thickening and increased orbital fat [1], while optic neuritis can cause optic nerve thickening [2]. Therefore, quantitative measurement of the orbital soft tissue volume plays a very important role in the study of orbital diseases.

Presently, the two-dimensional digital tomographic image sequence can be processed by using computers for transformation into a three-dimensional (3D) geometric model with an intuitive three-dimensional effect. Based on computed tomography (CT) or magnetic resonance imaging (MRI), the region of interest can be delineated and transformed into a 3D reconstruction model to calculate the volume of the study object using computer software [3–5]. This method is noninvasive, intuitive, and accurate and a highly feasible method to quantitatively measure tissue volume.

To our knowledge, the orbital soft tissue volume in Caucasians has been well studied, but only a few studies have reported on the normal orbital soft tissue volumes in Asians [6, 7]. However, there are differences in eye parameters between Asians and Caucasians [3–5, 8]. These differences are very unfavorable to study orbital soft tissue diseases in Asian patients with volume changes. This study intended to establish a 3D model of the orbital bony cavity and orbital soft tissues based on CT tomography images and calculate their volumes by computer software. We analyzed the relationship between the orbital soft tissue volumes and physical indicators such as sex, age, height, body weight, and body mass index (BMI). The purpose of this study was to obtain a reference range for the orbital soft tissue volume in Chinese adults.

2. Materials and Methods

2.1. Ethics Statement

This retrospective study was approved by the Ethical Review Committee of the First Affiliated Hospital of Guangxi Medical University and followed the tenets of the Declaration of Helsinki. Written informed consent was not required because this was a retrospective study.

2.2. Subjects

In this retrospective cross-sectional study, data were collected from patients who were admitted to the First Affiliated Hospital of Guangxi Medical University (located in Southern China) and underwent orbital CT from June 1, 2012, to December 1, 2015. The subjects were included in the study if the following conditions were met: (1) they were Chinese patients aged 18 years or older; (2) 16-slice CT scans were performed; and (3) the included patients had no abnormalities on one side.

Patients were excluded if they had the following conditions: (1) an orbital fracture caused by an external force that may have caused an injury that affected the contralateral eye (not necessarily discernible on CT); (2) orbital CT abnormalities were present on the side of interest, the presence of any soft tissue injury, previous eye surgery, high myopia (greater than 600 degrees), or any lesions that might affect the orbital bony cavity or orbital soft tissue (e.g., optic neuritis and strabismus); (3) the presence of thyroid disease, systemic diseases (e.g., hypertension and diabetes), immunologic diseases (e.g., idiopathic thrombocytopenic purpura and systemic lupus erythematosus), and systemic malignancies; and (4) patients with missing images or medical records and those not meeting the standard position requirements (e.g., head tilt or eye position skew).

We collected information on the sex, age, height, and body weight of the patients and calculated the BMI.

2.3. CT Scans

All patients were scanned using a 16-slice scanner (Somatom Sensation 16; Siemens Medical Solutions, Erlangen, Germany). A SIEMENS/Sensation 16-slice spiral CT scanner (Siemens Somatom Sensation 16 Computed Tomography) was used. The scanning parameters were as follows: tube voltage, 120 kV; tube current, 88.50 mA; layer thickness, 2.0 mm; slice spacing, 1.0 mm; rack tilt, 0°; and matrix, 512 × 512.

2.4. 3D Model Reconstruction

We collected original CT data in DICOM (Digital Imaging and Communications in Medicine) format through the picture archiving and communication systems of the hospital. The medical imaging software Mimics Research (20.0 version; Leuven, Belgium) was used for 3D reconstruction and volume calculation (Figure 1). We used the method proposed by Regensburg et al. [9] to perform 3D reconstruction of the orbital bony cavity, extraocular muscle, orbital fat, and intraorbital optic nerve, and then we calculated the orbital bony cavity volume (OV), extraocular muscle volume (MV), orbital fat volume (FV), and intraorbital optic nerve volume (iONV).

(a)

(b)

(c)

(d)

Figure 1: Axial CT slices with the highlighted segmented tissues and the three-dimensional reconstruction of the segmented issues: (a) extraocular muscle, (b) orbit fat, (c) intraorbital optic nerve, (d) orbital bony cavity.

2.5. Statistical Analysis

SPSS (SPSS 13.0 for Windows; SPSS Inc., Chicago, IL, USA) was used for the statistical analyses. The comparison of mean values of age, height, body weight, and BMI between men and women was performed using independent sample t tests. The mean OV, MV, FV, and iONV values and their ratios (MV/OV, FV/OV, and iONV/OV) were compared using multiple linear regression with and without age adjusted. The correlation between each parameter was analyzed using Pearson correlation analysis. All tests were two-tailed, and the significance level was set at .

3. Results

One-hundred three patients were enrolled in this study; they were aged 18–81 years, with a mean age (±SD) of 42 ± 15 years. Fifty-two male patients were aged from 18 to 69 years, and 51 female patients were aged from 18 to 81 years. The mean height, body weight, and BMI were 1.61 ± 0.07 m, 56 ± 9.9 kg, and 21 ± 3.3, respectively, for both genders (Table 1).

Table 1: Demographic characteristics.

Demographic data
Male (n = 52)
Female (n = 51)
value

Age, mean ± SD, years
37 ± 12
46 ± 17
0.002

Height, mean ± SD, cm
167 ± 5.3
156 ± 4.3
<0.001

Weight, mean ± SD, cm
60 ± 8.3
51 ± 9.7
<0.001

BMI, mean ± SD
22 ± 3.0
21 ± 3.5
0.586

BMI, body mass index; SD, standard deviation.

The results showed that OV, FV, MV, and iONV were all higher in Chinese males than in females (all ). Regarding the ratios, the MV/OV of males was higher than that of females (), while the differences in FV/OV () and iONV/OV () between males and females were not statistically significant (Table 2).

Table 2: Orbital fat volume, orbital muscle volume, orbital bony cavity volume, intraorbital optic nerve volume, and their ratios in normal Chinese orbits.

Male (n = 52)
Female (n = 51)
value

Unadjusted
Adjusted for age

OV, mean ± SD, cm³
22.2 ± 2.2
20.2 ± 1.5
<0.001
<0.001

FV, mean ± SD, cm³
8.89 ± 1.79
8.09 ± 1.74
0.024
<0.001

MV, mean ± SD, cm³
1.94 ± 0.34
1.57 ± 0.28
<0.001
<0.001

iONV, mean ± SD, cm³
0.405 ± 0.077
0.362 ± 0.074
0.005
0.036

FV/OV, mean ± SD
0.399 ± 0.063
0.398 ± 0.069
0.937
0.107

MV/OV, mean ± SD
0.0872 ± 0.0131
0.0782 ± 0.0130
0.001
0.017

iONV/OV, mean ± SD
0.0183 ± 0.0036
0.0180 ± 0.0037
0.702
0.516

FV, fat volume; iONV, intraorbital optic nerve volume; MV, muscle volume; SD, standard deviation; OV, orbital bony cavity volume.

With an increase in age, both MV (r = −0.349; ) and iONV (r = −0.279; ) decreased, but FV (r = 0.342; ) increased and OV (r = 0.014; ) remains unchanged (Figure 2).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

Figure 2: Scatterplots showing correlations between MV, FV, iONV, OV, and age, respectively (a-d), and correlations between MV, FV, iONV, OV, and BMI, respectively (e-h). FV, fat volume; iONV, intraorbital optic nerve volume; MV, muscle volume; OV, orbital bony cavity volume.

OV was positively correlated with height (r = 0.516; ) and body weight (r = 0.349; ) but was unrelated to BMI (r = 0.090; ) (Figure 2). FV was not associated with height (r = 0.148; ) but was positively correlated with body weight (r = 0.412; ) and BMI (r = 0.370; ). MV was positively correlated with height (r = 0.491; ), body weight (r = 0.475; ), and BMI (r = 0.264; ). iONV was positively correlated with height (r = 0.256; ) but was not associated with body weight (r = 0.067; ) and BMI (r = -0.070.; ).

4. Discussion

In this study, the mean OV of Chinese adult males was 22 ± 2.2 cm³, which was significantly higher than that of females (20 ± 1.5 cm³). Previous studies have demonstrated that the OV data in Asians, such as Hong Kong people in China (male: 22 ± 1.4 cm³; female: 20 ± 2.2 cm³) [10] and Koreans (22 ± 1.7 cm³ for men and 19 ± 1.8 cm³ for women) [11], were similar to ours. However, among Caucasians, the OVs (29 ± 2.4 cm³ for males and 25 ± 2.2 cm³ for females) [7] were greater than those for the population in this study. Correlation analysis results in this study showed that OV was positively correlated with height and body weight, consistent with the findings of Erkoç et al. [6] and Yoo et al. [12]. We speculate that the smaller OV in our study might be related to the mean body weight and height of Chinese people being smaller than those of Caucasians [13, 14].

Our study showed that with age, OV did not change, FV increased, and MV and iONV decreased. Darcy et al. [15] observed that orbital adipose tissue increased with age, a finding that was similar to ours. In this study, we found that MV decreased with age, consistent with the findings of Yoo et al. [12] and Regensburg et al. [7]. However, Tian et al. [16] reported different results, in which MRI images were used for calculation and indicated that MV was not related to age. The cause may be that the study by Tian et al. included only a few subjects (21 subjects, 42 orbits in either side).

Different from Regensburg et al.’s study [7] of Caucasians, we found that even the ratio of MV to OV (i.e., MV/OV) in males was significantly higher than that of females. Janssen et al. [17] found that the whole body muscle volume in male was significantly higher than that in female. Even with respect to BMI, this difference still exists. The study by Janssen et al. partially supported our findings. Whether there is a difference in the ratio of MV/OV between males and females requires confirmation in further studies.

Many optic nerve diseases, including optic neuritis, optic nerve atrophy, and optic nerve glioma, affect the optic nerve volume [18]. Previously, optic neuritis combined with optic nerve thickening was often misdiagnosed as an optic nerve tumor, and patients underwent unnecessary surgery [19]. Even today, the differential diagnosis of optic neuritis and ischemic optic neuropathy remains a challenge in certain cases, and the thickening of the intraorbital optic nerve is a key point to differentiate the two conditions [2]. Therefore, the range of iONV observed in this study can be used to facilitate the differentiation between these optic neuropathies, especially in the case without MRI.

This study has two limitations. First, the sample size was relatively small, so we did not group the patients by age. Second, in all cases, one side of the orbit is abnormal because the institutional review board did not approve CT scans for normal subjects for research purposes. However, we have attempted to rule out the effect of this abnormal side on the contralateral orbit to be studied.

5. Conclusion

In comparison with previous studies, OV, MV, and FV in Chinese tend to be lower than those in Caucasians. This study determined that the MV, FV, and iONV for males were all higher than those for females; even for the ratio of MV/OV, the value for males was still higher than that for females. With an increase in BMI, both MV and FV increased, but iONV did not show this trend.

Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Authors’ Contributions

Yi Du and Bing-Yao Lu have contributed equally to this work and should be considered as co-first authors.

Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81560162) and Natural Science Foundation of Guangxi Zhuang Autonomous Region (Nos. 2016GXNSFAA380301 and 2018GXNSFAA050052).

References

N. I. Regensburg, W. M. Wiersinga, T. T. J. M. Berendschot, P. Potgieser, and M. P. Mourits, “Do subtypes of graves’ orbitopathy exist?” Ophthalmology, vol. 118, no. 1, pp. 191–196, 2011.

A. T. Toosy, D. F. Mason, and D. H. Miller, “Optic neuritis,” The Lancet Neurology, vol. 13, no. 1, pp. 83–99, 2014.

L. G. Farkas, M. J. Katic, and C. R. Forrest, “International anthropometric study of facial morphology in various ethnic groups/races,” Journal of Craniofacial Surgery, vol. 16, no. 4, pp. 615–646, 2005.

X.-S. Wu, X.-C. Jian, Z.-J. He, X. Gao, Y. Li, and X. Zhong, “Investigation of anthropometric measurements of anatomic structures of orbital soft tissue in 102 young han Chinese adults,” Ophthalmic Plastic & Reconstructive Surgery, vol. 26, no. 5, pp. 339–343, 2010.

Y. Ji, Z. Qian, Y. Dong, H. Zhou, and X. Fan, “Quantitative morphometry of the orbit in Chinese adults based on a three-dimensional reconstruction method,” Journal of Anatomy, vol. 217, no. 5, pp. 501–506, 2010.

M. F. Erkoç, B. Öztoprak, C. Gümüş, and A. Okur, “Exploration of orbital and orbital soft-tissue volume changes with gender and body parameters using magnetic resonance imaging,” Experimental and Therapeutic Medicine, vol. 9, no. 5, pp. 1991–1997, 2015.

N. I. Regensburg, W. M. Wiersinga, M. E. J. van Velthoven et al., “Age and gender-specific reference values of orbital fat and muscle volumes in Caucasians,” British Journal of Ophthalmology, vol. 95, no. 12, pp. 1660–1663, 2011.

J. Kunjur, T. Sabesan, and V. Ilankovan, “Anthropometric analysis of eyebrows and eyelids: an inter-racial study,” British Journal of Oral and Maxillofacial Surgery, vol. 44, no. 2, pp. 89–93, 2006.

N. I. Regensburg, P. H. B. Kok, and F. W. Zonneveld, “A new and validated CT-based method for the calculation of orbital soft tissue volumes,” Investigative Ophthalmology & Visual Science, vol. 49, no. 5, pp. 1758–1762, 2008.

A. Chau, K. Fung, L. Yip, and M. Yap, “Orbital development in Hong Kong Chinese subjects,” Ophthalmic and Physiological Optics, vol. 24, no. 5, pp. 436–439, 2004.

S.-P. Kim, B.-Y. Lee, S.-J. Lee et al., “A study on orbital volume of Korean people in their 20s or 40s,” Ophthalmic Research, vol. 47, no. 2, pp. 98–102, 2012.

J. H. Yoo, Y. H. Lee, H. Lee et al., “Correlation between orbital volume, body mass index, and eyeball position in healthy East asians,” Journal of Craniofacial Surgery, vol. 24, no. 3, pp. 822–825, 2013.

List of average human height worldwide. 2019, https://en.wikipedia.org/wiki/List_of_average_human_height_worldwide.

Human body weight, 2019, https://en.wikipedia.org/wiki/Human_body_weight.

S. J. Darcy, T. A. Miller, R. A. Goldberg, J. P. Villablanca, J. L. Demer, and G. H. Rudkin, “Magnetic resonance imaging characterization of orbital changes with age and associated contributions to lower eyelid prominence,” Plastic and Reconstructive Surgery, vol. 122, no. 3, pp. 921–929, 2008.

S. Tian, Y. Nishida, B. Isberg, and G. Lennerstrand, “MRI measurements of normal extraocular muscles and other orbital structures,” Graefe’s Archive for Clinical and Experimental Ophthalmology, vol. 238, no. 5, pp. 393–404, 2000.

I. Janssen, S. B. Heymsfield, Z. M. Wang, and R. Ross, “Skeletal muscle mass and distribution in 468 men and women aged 18–88 yr,” Journal of Applied Physiology, vol. 89, no. 1, pp. 81–88, 2000.

R. G. Peyster, E. D. Hoover, B. L. Hershey, and M. E. Haskin, “High-resolution CT of lesions of the optic nerve,” American Journal of Roentgenology, vol. 140, no. 5, pp. 869–874, 1983.

G. Uccello, P. Fedriga, F. Tranfa, P. Vassallo, and G. Bonavolonta, “CT scan in the differential diagnosis of thickened optic nerve AU—Uccello,” Orbit, vol. 5, no. 4, pp. 255–258, 1986.