Excitation-Dependent Fluorescence Quantum Yield for Freshwater Chromophoric Dissolved Organic Matter from Northern Russian Lakes

Advanced fluorescence analysis within the wide range of excitation wavelengths from 230 to 510 nm accompanied with chromatography was used to study natural chromophoric dissolved organic matter (CDOM) from three freshwater Karelian lakes. %e influence of excitation wavelength (λex) on fluorescence quantum yield and emission maximum position was determined.%e CDOM fluorescence quantum yield has reached a minimum at λex∼270–280 nm and a maximum at λex∼340–360 nm. It was monotonously decreasing after 370 nm towards longer excitation wavelengths. Analytical reversed-phase high-performance liquid chromatography with multiwavelength fluorescence detector characterized distribution of fluorophores between hydrophilic/hydrophobic CDOM parts.%is technique revealed “hidden” protein-like fluorophores for some CDOM fractions, in spite of the absence of protein-like fluorescence in the initial CDOM samples.%e humic-like fluorescence was documented for all hydrophilic and hydrophobic CDOM chromatographic peaks, and its intensity was decreasing along with peaks’ hydrophobicity. On contrary, the protein-like fluorescence was found only in the hydrophobic peaks, and its intensity was increasing along with peaks’ hydrophobicity. %is work provides new data on the CDOM optical properties consistent with the formation of supramolecular assemblies controlled by association of low-molecular size components. In addition, these data are very useful for understanding the CDOM function in the environment.


Introduction
Chromophoric dissolved organic matter (CDOM) naturally occurring in all aquatic environments is colored and contains materials of different molecular sizes (MSs).ese chemically complex compounds of irregular structure exert physical-chemical properties of water, affect sorption capacity, and control transport of pollutants in different environments.
e CDOM absorbs light in the UV and shortwave visible ranges and thus governs the optical properties for both freshwater and marine aquatic ecosystems directly influencing the spectral quality of the underwater light field.Because of its absorbance in the UV, it can protect aquatic organisms being damaged by the UV radiation [1].
Emittance of fluorescence under UV light is one of the main properties of aquatic CDOM that allows its fast and highly sensitive analysis [2][3][4][5][6][7][8].Fluorescence measurements provide essential information about the chemical structure of aquatic CDOM and require small sample amounts.It can be applied also in the remote sensing using lidar systems for CDOM fluorescence detection from aboard the ship or aircraft.As it is currently accepted, the fluorescence of aquatic CDOM consists of two main bands, the so-called protein-like and humic-like fluorescence coming from two main groups of fluorophores [2,3].Protein-like fluorophores usually have an excitation and emission maxima below 305 and 380 nm, respectively.e other group corresponds to humic-like fluorophores and shows emission within the wavelength range 380-600 nm upon excitation within the wavelength range 230-550 nm.Along with changing the excitation wavelength (λ ex ) from 270 to 310 nm, the humic-like emission maximum shifts towards shorter wavelengths.Such a shift of the emission maximum with increasing excitation wavelength was named the "blue shift" [4]. is phenomenon has been described for various types of natural waters [5][6][7][8] and during transformations of humic substances caused by microscopic fungi [9].
e fluorescence quantum yield of SDOM has been an object of several studies where it was found that it may vary depending on the excitation wavelength [7,10,11].Its values under excitation at discrete wavelengths 270, 310, and 355 nm were measured for the fractions of different MSs obtained by filtration on 5 nm pore size membrane in riverine CDOM [5,6] and various freshwater and marine samples [7].Low-MS fractions (<5 nm) demonstrated higher fluorescence quantum yield in comparison with the high-MS fractions (>5 nm).Fluorescence quantum yield was found essentially dependent of excitation wavelength with increasing its value along with the excitation wavelength rising from 270 to 355 nm.Contrasting to aquatic CDOM, the terrestrial humic acids and industrial humic preparations showed lower fluorescence quantum yield, values which practically did not depend on excitation wavelength altered within the range 270-355 nm [7,12].However, until now, the chemical structure of fluorophores of aquatic CDOM still remains unclear.Yet, to clear the localization of fluorescent species between different structural compounds should be very useful to understand the origin of SDOM fluorescence and its functioning in aquatic environments.
e current work is targeted to fluorescence measurements within the wide range of excitation wavelengths from 230 to 510 nm focusing on fluorescence quantum yield estimation.We used the reversed-phase high-performance liquid chromatography (RP-HPLC) with online multiwavelength absorbance and fluorescence detections to analyze protein-like and humic-like fluorophores of the CDOM from several lakes of northern Russia with the aim to generalize fluorescence distribution between hydrophilic/hydrophobic parts of CDOM in freshwater lakes.

CDOM Sample Preparation.
e samples of surface water were taken during an expedition (August 2013) in several freshwater lakes located in Karelia, the north of the European part of Russia.
e Onego lake is the second largest freshwater body in Europe.In recent years, human impact on the ecosystem of Onego lake has increased, especially in the northwestern part, where several industrial areas are located.e two small ultrafresh lakes of marsh origin, Verhnee and Vodoprovodnoe, are located far from industrial facilities and can serve as a source of CDOM in the absence of anthropogenic load.Water samples were stored in the sealed plastic bottles in the dark at +4 °C.e 100 ml of original freshwater samples were filtered using cellulose filters (Millipore) with a pore size of 0.45 μm.50 mL of each filtered natural water sample was freeze dried and dissolved in 1.5-3 ml of 10 mM phosphate buffer (pH 6.5) prepared by mixing three volumes of 10 mM Na 2 HPO 4 solution (pH 9.8) and seven volumes of 10 mM NaH 2 PO 4 solution (pH 4.5).
e concentrated stock solutions of CDOMs were stored in dark glass vials at +4 °C before further spectroscopic and chromatographic analyses.

UV-Visible Absorption and Fluorescence Emission
Spectra.
e absorption spectra of original lake water, filtered samples, and CDOM dissolved in phosphate buffer were measured in a quartz cuvette with optical path 1 cm or 3 cm using the UV-Vis spectrophotometer Solar PB-2201 (Belarus) within wavelength range 200-900 nm. e 3 cmcell was used to measure absorbance more accurately in the visible range of the spectrum.For further applications, all absorbances were related to 1 cm.e fluorescence emission spectra of CDOM samples were recorded in 1 cm standard quartz cuvette using a Cary Eclipse fluorescence spectrophotometer (Varian, USA) at different excitation wavelengths from 230 to 510 nm with the interval of 10 nm within the emission range of 200-700 nm.In order to minimize the inner-filter effect, all samples were diluted with 0.1 mM phosphate buffer (pH 6.5) to reach an absorbance of 0.05 at 270 nm.

Fluorescence Quantum Yield Measurement.
e fluorescence quantum yield was measured by the method of the reference compound applied earlier for the samples of CDOM in natural water and commercial humic preparations [6,7,12].As the standard reference compound, quinine sulfate in aqueous 0.05 M O 2 SO 4 solution was used because it is similar to aquatic humic substances in the shape of emission spectrum with the emission maximum at 450 nm. e fluorescence quantum yield of quinine sulfate is well known and equals to 0.546, or 54.6% under excitation at 350 nm [13].To obtain a calibration curve, the fluorescence spectra of quinine sulfate were measured at λ ex � 350 nm and concentrations 3•10 −7 , 5•10 −7 , and 7•10 −7 M.
e details of the chromatographic procedure were described in [14,15].e fluorescence of RP-HPLC chromatograms were monitored using an Acquity fluorescence detector (FLR) with λ ex � 270 nm and emission wavelength range 300-600 nm.
e chromatograms with online emission detection at 330 and 450 nm were analyzed for detection of protein-like and humic-like fluorescence in 2 Journal of Spectroscopy the same chromatographic run.
e elution rate was 0.3 mL/min.Methanol and 10 mM phosphate buffer (pH 6.5) were used to form a stepwise gradient.
e fluorescence spectra for each RP-HPLC chromatographic peaks' top were extracted from the data of FLR detector.

UV-Visible Absorption Spectra.
e absorption spectra of each original and filtered freshwater sample were similar, demonstrating the low level of organic material in the suspended form presented in the original natural water (Figure 1).All absorption spectra for original water and filtered samples were featureless with a monotonic decline with wavelength increasing from 200 to longer wavelengths with the shoulder located around 260-290 nm. e CDOM content in the lake Vodoprovodnoe was about twice higher than that in two other lakes.e absorption spectra differed in the slope of the curve as well.e absorbance ratio at 230 and 350 nm (A230/A350) was taken as a quantitative slope measurement.is ratio was used to demonstrate essential differences between the content of aliphatic bonds S-S, S-O, and S-P in CDOM of the lakes investigated.e highest value of A230/A350 ratio was noticed for the CDOM in the Onego lake (6.70) and was considerably less (4.77 and 5.44) for the lakes Vodoprovodnoe and Verhnee, respectively.It means that the CDOM of the Onego lake is enriched in aliphatic compounds compared to other two lakes.

e Fluorescence Spectra and the Humic-Like Fluorescence Emission Maximum as a Function of the Excitation
Wavelength.
e emission spectra of the CDOM from three Karelian lakes excited at wavelengths from 230 to 510 nm are presented in Figure 2 after subtraction of the blank emission at each excitation wavelength.We observed that the humiclike fluorescence was prevailing in all CDOM samples.In the excitation region 230-280 nm, the CDOM exhibited broad humic-like bands with the emission maxima from 425 to 450 nm and the protein-like fluorescence as a shoulder around 330 nm. e intensity of protein-like emission was higher being excited at λ ex 230, 270, and 280 nm. e ratio    between protein-like and humic-like emission intensity was the highest in the Onego lake CDOM and much less in two other samples.
e fluorescence spectra of studied CDOM samples from the lakes manifested a continuous red shift of humiclike emission maximum with increasing λ ex from 310 to 510 nm (Figure 3), which is a common feature of the CDOM from rivers and coastal margins receiving terrestrial input [16,17].Additionally, all studied CDOM samples showed the red shift of humic-like emission maximum with increasing λ ex from 230 to 270-280 nm, within the excitation range not yet analyzed before by other researchers.e so-called "blue shift" was observed with changing λ ex from 270 to 310 nm, which is typical for natural water [5][6][7].We did not find any information published in scientific literature about excitation dependency of emission wavelength for the CDOM in freshwater lakes in the excitation range 230-510 nm, so we believe that our results are the first to report this.

e Fluorescence Quantum Yield as a Function of Excitation Wavelength.
e fluorescence quantum yield values for the CDOM of three freshwater lakes showed nonmonotonous dependence with variation of λ ex from 230 to 510 nm (Figure 4).e highest fluorescence quantum yields in all the samples (2.3-3.4%) were obtained with λ ex around 340-360 nm.After increasing λ ex from 370 to 510 nm, quantum yield values decreased monotonically, demonstrating shoulders in 380-410 and 420-470 nm.From λ ex 230 to 270-280 nm, the quantum yield values decreased to 1.2-1.7% and again increased to the maximum at 340-360 nm.It should be noticed that the local minimum was detected in the similar excitation wavelength range (270-290 nm), where the most significant "blue shift" was found. is dependence is in agreement with our previous measurements of quantum yield values on coastal surface waters of the Kara Sea in the excitation region 250-550 nm [8].
e fluorescence quantum yield measurement is sometimes used to characterize aquatic CDOM of marine, riverine, or coastal origin.
e overall shape of dependencies of fluorescence quantum yield on excitation wavelength was found to be similar in different CDOM samples: the maximum fluorescence efficiencies were obtained at excitation wavelengths between 340 and 380 nm.
In [17], the dependence of fluorescence quantum yield on excitation wavelength was used as an evidence of the presence of terrestrial material in natural waters across the Equatorial Atlantic Ocean.e fluorescence quantum yield increased from 280 nm to a maximum at 370-380 nm ranging from 1.8 to 2.5% and decreased monotonically thereafter.e fluorescence quantum yield of CDOM in waters from south Florida, Amazon, and Tamiami rivers and fulvic acids isolated from the Suwannee River was measured by Green and Blough [18].During investigation performed on water samples from the Equatorial Atlantic Ocean [19], it was established that for excitation wavelengths above 375 nm, the fluorescence quantum yield decreased with decreasing λ ex and the emission maximum shifted continuously to the red.Additionally, maximum at ∼340 nm was observed for the CDOM from a site influenced by the Congo River Plume.e similar behavior of fluorescence quantum yield of CDOM in deep and surface waters of the Norwegian Sea with maxima between 340 and 380 nm was reported by Wünsch et al. [20].In whole, our data corroborate with the other studies performed.However, our results presented excitation dependence of the CDOM fluorescence quantum yield in the wavelength range below 280 nm for freshwater lakes CDOM for the first time.is is important because the minimum fluorescence quantum yield around excitation at 280 nm was found.Moreover, it is valuable for further interpretation of CDOM fluorescence origin because it is the same spectral range of excitation where the blue shift of CDOM fluorescence emission is normally observed.

e Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) with Online Absorbance and Fluorescence Detections.
e RP-HPLC with stepwise gradient  Journal of Spectroscopy formation using methanol and aqueous phosphate buffer gave possibility to map the CDOM in terms of distribution of hydrophilic/hydrophobic chromophores.e chromatograms with online absorbance detection resolved six peaks with different hydrophobic properties.e hydrophobicity of peaks increased from the first to the last eluted peak due to the increase of methanol concentration in the stepwise separation procedure.In our previous study [21], we have found that the highest content of hydrophilic fraction was in the CDOM from the Onego lake.In this research, we have confirmed this finding for all studied lakes by registration of online fluorescence (Figure 5).e fluorescence detection at λ ex � 270 nm has shown that the CDOM in all three lakes contained humic-like fluorophores, for which the emission maxima depended on hydrophilic/hydrophobic properties.
e most intensive humic-like fluorescence with emission maximum at 420 nm was found for the first hydrophilic peak in all three samples.Along with increasing the hydrophobicity of chromatographic fraction, the humic-like fluorescence maximum shifted in 30 nm towards longer wavelengths.So RP-HPLC revealed the presence of at least two humic-like fluorophores with different emission maxima (420 or 450 nm) depending on their hydrophobicity.Fluorescence measurements were made not only for humic-like fluorescence but also for protein-like fluorescence.
e fluorophore emitting a maximum at around 345 nm was found in the most hydrophobic fractions of CDOM.
e highest amplitude of protein-like fluorescence was detected in the CDOM chromatographic peaks #4-6 of the Onego lake (Figures 5(b) and 5(d)), while in CDOM from two other lakes of marsh origin, the protein-like fluorophores were found in the peaks #5-6 only in the trace amounts compared to humic-like fluorophores (Figures 5(a) and 5(c)).

Observation of "Hidden" Fluorophores Emitting Protein-Like Fluorescence.
e application of the RP-HPLC revealed "hidden" protein-like fluorophores in hydrophobic CDOM peaks.e local minimum in fluorescence quantum yield dependency on excitation wavelength for freshwater CDOM was found at λ ex ∼270-280 nm, where the "blue shift" of CDOM fluorescence emission was typically observed as well e CDOM from the Vodoprovodnoe lake demonstrated similar chromatograms and emission spectra with that of the Verhnee lake.[5][6][7].On the contrary, the RP-HPLC with online fluorescence detection has shown that the intensive humic-like fluorescence was found at all hydrophilic and hydrophobic chromatographic peaks (Figure 5).It should be stressed that the intensity of protein-like fluorescence increased with the increase of peaks' hydrophobicity, while the humic-like fluorescence decreased.is effect was more pronounced in the Onego lake CDOM (Figures 5(b) and 5(d)).High amount of hydrophobic "hidden" protein-like fluorophores (i.e., fluorophores clearly detected by RP-HPLC but not so obviously visible in the original CDOM fluorescence spectra before fractionation) is one of the reasons of humic-like fluorescence quenching.We believe that the minimal CDOM fluorescence quantum yield observed at λ ex ∼270-280 nm could be partially from absorption of light by chromophoric groups similar to aromatic amino acids, short peptides, or proteins and thus leading to reduction in humic-like fluorescence excited at this wavelength range.

Conclusions
Fluorescence properties of natural CDOM from three Karelian freshwater lakes were examined within the excitation wavelength range 230-510 nm, and the influence of λ ex on fluorescence quantum yield and wavelength of emission maximum was determined.For all studied CDOM samples, the "blue shift" of humic-like fluorescence was observed at λ ex from 270 to 310 nm.On the contrary, a continuous "red shift" in emission maximum with increasing excitation wavelength was observed at λ ex from 230 to 270 nm and ≥310 nm. e quantum yield of CDOM fluorescence decreased starting from excitation at 230 nm, showing a minimum at 270-280 nm, then reaching a maximum at 340-360 nm and further decreasing towards longer excitation wavelengths.ese results, for the first time, demonstrated the excitation-wavelength dependence of fluorescence quantum yield in the spectral range of excitation below 280 nm for freshwater CDOM.Analytical RP-HPLC with multiwavelength fluorescence and absorbance detectors was used to characterize CDOM in freshwater lakes and to determine distribution of fluorophores between hydrophilic/hydrophobic CDOM parts.e intensive humiclike fluorescence was found at all hydrophilic and hydrophobic chromatographic peaks, while the protein-like fluorescence was detected only in the most hydrophobic peaks; the intensity of protein-like fluorescence increased with the increase of peaks' hydrophobicity, while the humic-like fluorescence decreased.
ese results are well consistent with the formation of supramolecular assemblies by the CDOM molecules, likely controlled by association of low-molecular size components.e distribution of humic-like and protein-like fluorophores within the CDOM provides detailed information that may help to better trace the origin of CDOM and its biogeochemical transformations in the environment.

Figure 1 :
Figure 1: Absorption spectra of surface freshwater from three Karelian lakes, unfiltered (solid lines) and filtered (dashed lines).

Figure 3 :Figure 4 :
Figure 3: Emission maximum wavelength for the humic-like fluorescence as dependence on excitation wavelength for CDOM samples from three Karelian lakes.

Figure 5 :
Figure 5: RP-HPLC chromatograms of CDOM from the Verhnee (a) and Onego (b) lakes with online fluorescence detection at excitation/emission wavelength pairs 270/450 nm (red lines) and 270/350 nm (blue lines) for detection of humic-like and protein-like fluorescence, respectively.Inset zoom shows the zone of chromatograms from 3.5 to 8.5 min.Normalized emission spectra of the peaks #1-6 of fractionated CDOM from the Verhnee (c) and Onego (d) lakes.eCDOM from the Vodoprovodnoe lake demonstrated similar chromatograms and emission spectra with that of the Verhnee lake.