Mediators of Inflammation
Volume 2019 (2019), Article ID 9198518, 6 pages
https://doi.org/10.1155/2019/9198518

Corrigendum

Corrigendum to “Effect of High, Medium, and Low Molecular Weight Hyaluronan on Inflammation and Oxidative Stress in an In Vitro Model of Human Nasal Epithelial Cells”

Giusy Daniela Albano,¹ Anna Bonanno,¹ Luca Cavalieri,² Eleonora Ingrassia,² Caterina Di Sano,¹ Loredana Riccobono,¹ Rosalia Gagliardo,¹ and Mirella Profita¹

¹Institute of Biomedicine and Molecular Immunology “A. Monroy” (IBIM), National Research Council of Italy (CNR), Via Ugo La Malfa 153, 90146 Palermo, Italy
²Chiesi Farmaceutici S.p.A, Parma, Italy

Correspondence should be addressed to Mirella Profita; profita@ibim.cnr.it

Received 9 April 2019; Accepted 14 April 2019; Published 3 October 2019

Copyright © 2019 Giusy Daniela Albano et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

In the article titled “Effect of High, Medium, and Low Molecular Weight Hyaluronan on Inflammation and Oxidative Stress in an In Vitro Model of Human Nasal Epithelial Cells” [1], there was a duplication of bands and undeclared splicing in the Western Blots. After this was raised on PubPeer, the following concerns were confirmed:

(i)Figure 1(a) β-actin lanes 1 and 2 appear to be the same as Figure 4 β-actin lanes 1 and 2. They represent the same conditions (untreated cells and rh-IL-17A), but the duplication was not declared. Figure 1(a) β-actin lanes 1-3 are also the same as Figure 2 β-actin lanes 1-3, though the latter has been compressed horizontally to about 70% of the width. The third lane is not the same experiment.(ii)Figure 1(a) pERK lanes 1 and 2 are the same as Figure 3(b) pERK lanes 1 and 2, and they appear to represent the same conditions.(iii)Figure 1(b) β-actin lanes 1 and 2 are the same as Figure 3(b) β-actin lanes 1 and 2. Figure 1(b) pIκBα lanes 1 and 2 are the same as Figure 3(b) pIκBα lanes 1 and 2. However, the ratios reported in the respective bar charts above the blots are quite different.(iv)Figure 2(b) β-actin lanes 1 and 2 are the same as Figure 4(b) lanes 1 and 2, though the latter has been compressed horizontally to about 70% of the width.

The authors apologized for the confusion and said this was due to inadvertent human error. They prepared a new set of figures, which have no duplication. A declaration of splicing between the bands is included in the legends of Figures 1(a) and 2(b). The corrected figures, as approved by the editorial board, are as follows:

(a)

(b)

Figure 1: Effect of the U0126 inhibitor on ERK and IκBα phosphorylation in RPMI 2650 cells stimulated with rhIL-17A. The cells were stimulated with rhIL-17A (20 ng/mL) or PMA (50 ng/mL) for 30 min in the absence or presence of U0126 (25 μM). (a) pERK and pIκBα protein expressions were evaluated in the cell lysates by western blot. The results were expressed as the ratio of band intensity and β-actin of 3 separate experiments. Representative gel images of pERK, pIκBα, and β-actin are shown. The bands are spliced from uncropped blots. (b) The activation of ERK1/2 and NF-κB for each experimental condition was tested for the pERK1/2/total ERK1/2 ratio and for the pNF-κB/total NF-κB, respectively, by ELISA, and normalized for protein content. ANOVA with Fisher’s test correction was used for the analysis of the data. was statistically significant.

(a)

(b)

Figure 2: Effect of the U0126 inhibitor in RPMI 2650 cells stimulated with rhIL-17A. (a) The cells were stimulated with rhIL-17A (20 ng/mL) for 6 hrs in the absence or presence of U0126 (25 μM). ROS production was evaluated in the cells by flow cytometry. The bars represent the of 3 separate experiments. Representative flow cytometry is shown; (b) the cells were stimulated with rhIL-17A (20 ng/mL) for 18 hrs in the absence or presence of U0126 (25 μM). NOX-4 and IL-8 protein expressions were evaluated in the cell lysates by western blot. The results were expressed as the ratio of band intensity and β-actin of 3 separate experiments. Representative western blot is shown. The bands are spliced from uncropped blots. ANOVA with Fisher’s test correction was used for the analysis of the data. was statistically significant.

(a)

(b)

Figure 3: Effect of HMW-HA, MMW-HA, and LMW-HA on ERK1/2 and NF-κB signal pathway in RPMI 2650 cells stimulated with rhIL-17A. The cells were preincubated with HMW-HA (100 μg/mL), MMW-HA (100 μg/mL), and LMW-HA (100 μg/mL) for 1 h and then stimulated with rhIL-17A (20 ng/mL) for 30 min; (a) pERK and pIκBα protein expressions were evaluated in the cell lysates by western blot. The bars represent the ratio of band intensity and β-actin of 3 separate experiments. Representative gel images of pERK, pIκBα, and β-actin are shown; (b) the activation of ERK1/2 and NF-κB for each experimental condition was tested for the pERK1/2/total ERK1/2 ratio and pNF-κB/total NF-κB ratio by ELISA and normalized for protein content. ANOVA with Fisher’s test correction was used for the analysis of the data. was statistically significant.

(a)

(b)

Figure 4: Effect of HMW-HA, MMW-HA, and LMW-HA in RPMI 2650 cells stimulated with rhIL-17A. (a) The cells were preincubated with HMW-HA (100 μg/mL), MMW-HA (100 μg/mL), and LMW-HA (100 μg/mL) for 1 h and then stimulated with rhIL-17A (20 ng/mL) for 6 hrs. ROS production was evaluated in the cells by flow cytometry. The bars expressed the of 3 separate experiments. Representative flow cytometry is shown; (b) the cells were preincubated with HMW-HA (100 μg/mL), MMW-HA (100 μg/mL), and LMW-HA (100 μg/mL) for 1 h and then stimulated with rhIL-17A (20 ng/mL) for 18 hrs. NOX-4 and IL-8 protein syntheses were evaluated in the cell lysates by western blot. The bars represent the ratio of band intensity and β-actin of 3 separate experiments. Representative western blot is shown. ANOVA with Fisher’s test correction was used for the analysis of the data. was statistically significant.

(a)

(b)

Figure 5: Effect of U0126, HMW-HA, MMW-HA, and LMW-HA on nuclear translocation of NF-κB in RPMI 2650 cells stimulated with rhIL-17A. The cells were preincubated (a) with U0126 (25 μM) or (b) with HMW-HA (100 μg/mL), MMW-HA (100 μg/mL), and LMW-HA (100 μg/mL) for 1 h and then stimulated with rhIL-17A (20 ng/mL) for 30 min; NF-κB was evaluated in nuclear cell lysate by western blot. The bars represent the ratio of band intensity and β-actin of 3 separate experiments. Representative gel images of NF-κB and β-actin are shown. ANOVA with Fisher’s test correction was used for the analysis of the data. was statistically significant.

Acknowledgments

Dr. Liboria Siena, the sixth author of the article, sadly passed away in November 2016.

Supplementary Materials

The uncropped gels showing the selected bands for Figures 1(a), 2(b), 3(a), and 4(b). (Supplementary Materials)

References

G. D. Albano, A. Bonanno, L. Cavalieri et al., “Effect of high, medium, and low molecular weight hyaluronan on inflammation and oxidative stress in an in vitro model of human nasal epithelial cells,” Mediators of Inflammation, vol. 2016, Article ID 8727289, 13 pages, 2016.