Antimalarial Effects of Iranian Flora Artemisia sieberi on Plasmodium berghei In Vivo in Mice and Phytochemistry Analysis of Its Herbal Extracts

The aim of this study is pharmacochemistry of Iranian flora Artemisia sieberi and its antimalarial effects on Plasmodium berghei in vivo. This is the first application of A. sieberi for treatment of murine malaria. A. sieberi were collected at flowering stage from the Khorassan and Semnan provinces of Iran; the aerial parts were air-dried at room temperature and then powdered. The powder was macerated in methanol, filtered with Bokhner hopper and solvent was separated in rotary evaporator. Total herbal extract was subsequently processed for ether and chloroform extracts preparation. The toxicity of herbal extract was assessed on naive NMRI mice with high, average and low doses; then pathophysiological signs were assessed. Finally, the antimalarial efficacy was investigated on two groups of Plasmodium berghei infected mice. Percentage of parasitaemia and pathophysiology were also evaluated. The results of this assessment showed no toxicity even by high concentration of herbal extract. A significant reduction in percentage of parasitaemia was observed; no alterations of hepatosplenomegaly and body weight were indicated in study group. A. sieberi extracts showed antimalarial effects against murine malaria with some efficacies on reducing pathophysiology. However, there is requirement to find the major component of this herbal extract by further studies.


Introduction
Malaria is one of the most serious and widespread diseases encountered by human. It is an infectious disease caused by the parasite Plasmodia (P.) transmitted by the female anopheles. Four identified species of this parasite exist, which cause different types of human malaria [1]. Although all the four species of malaria parasites can infect humans and cause illness, only P. falciparum is known to be potentially life threatening and some of infected persons die, usually because of delayed treatment [2]; however, annual incidence of clinically new cases and mortality rates are decreasing [3][4][5][6].
As malaria vaccines remain problematic, chemotherapy still is the most important weapon in the fight against the disease [7]. The antimalarial drugs including chloroquine, quinine, mefloquine, pyrimethamine, and artemisinin are currently used in malaria treatment. Part of the reason for the failure to control malaria is the spread of resistance to firstline antimalarial drugs, cross-resistance between the limited number of drug families available, and some multidrug resistance [8]. Resistance has emerged to all classes of antimalarial drugs except artemisinin, an endoperoxide antimalarial drug derived as the active component of Artemisia annua, a herbal remedy used in Chinese folk medicine for 2000 years "qinghaosu" [9][10][11][12]. Artemisinin is a natural product and a powerful antimalarial drug with significant activities, which has high potency whilst possessing low toxicity during treatment of malaria [13][14][15].
The genus Artemisia has always been of great pharmaceutical interest and is useful in traditional medicines for  Figure 1: Toxicity assay induced by A. sieberi crude extract in naive animals. Pathophysiological alterations including body weight, survival rate, and hepato/splenomegaly were evaluated in control and test groups as toxicity assay induced by injection of low, average, and high doses of A. sieberi crude extract (n = 5 mice/group, Student's t-test, * P < 0.05).
a treatment of the variety of diseases [11,16,17]. A. annua is presently being cultivated on a commercial scale in China and Vietnam for its antimalarial sesquiterpene lactone. The genus is of small herbs found in Northern temperate regions and belongs to the important family Compositae (Asteraceae), which comprises about 1,000 genera and over 20,000 species. Within this family, Artemisia is included into the family Anthemideae and comprises itself over 400 species, found in Europe and North America, but mainly are dominating Asia [18][19][20]. Among the Asian Artemisia flora, 150 species were recorded for China, 50 species reported in Japan, and 34 species found in Iran, of which may be endemic: A. melanolepis Boiss and A. kermanensis Pold [21], A. absinthium [22], A. annua [23], A. dracunculus [24], A. aucheri [25], A. haussknechtii Boiss [26], A. scoparia, A. sieberi [27], and A. sieberi Besser [28]. Pharmacochemical analysis of Artemisinin shows that the structure of this compound is rather unique among natural products as it contains the very unusual 1,2,4-trioxane ring system. It was sufficiently unusual that it was originally characterized as an ozonide until revised crystallographic analysis provided unambiguous structural elucidation [29][30][31][32][33]. For a drug to be effective against the malaria parasite, it must reach the site of action in sufficient concentration and then interact with the receptors before it is either deactivated and/or eliminated by the host or the parasite. Pharmacological and biochemical evaluation revealed that this compound was a blood schizonticide, preferentially imported into malaria infected erythrocytes via the parasitophorous duct [34] as it has been also shown in noninfectious diseases [35]. Due to complex chemical structure of artemisinin, the chemical synthesis of the molecule is complex, which results in very low yields, and the cost becomes prohibitory to use synthetic approach for its commercial production [36]. The mechanism of the action of Artemisinin remains a mystery; several candidates have been hypothesized as targets Control Test (d) Figure 2: Toxicity assay induced by A. sieberi crude extract in malarial animals. Pathophysiological alterations including body weight, survival rate, and hepato/splenomegaly were evaluated as indices of toxicity by crude extract of A. sieberi in control and malarial groups (test, A. sieberi crude extract; control, drug vehicle; n = 10 mice/day/group, Student's t-test).

Plant Samples.
The aerial parts of A. sieberi were collected at flowering stage from the Khorassan and Semnan provinces of Iran. Voucher specimens were deposited and identified at the Herbarium of the Research Institute of Forests and Rangelands (RIFR), Tehran, Iran.

Herbal Extraction.
The method was applied as described previously [52]. The aerial parts were air dried at room temperature then were powdered by mixer. The powder (140 gr) of A. sieberi was macerated in 1 lit methanol (Merck) and then kept for 72 h away from light and high temperature. It was filtered, evaporated, and dried by Rotary evaporator (Eyela, N-1000, Japan) and finally defatted in refrigerator. Wet weight of raw extract at the final step was 13.3 gr, and its color was dark green. The extract was kept in refrigerator until applied for the toxicity assay.

Ether and Chloroform Extraction of A. sieberi Compounds.
Herbal extract was eluted with 300 mL n-hexane (Sigma, Co. India); two phases were separated; the lower hexane phase (non-polar compounds) was collected and kept at refrigerator for further experiment. The upper phase was eluted with 300 mL chloroform (Merck, India) 3 times; subsequently lower chloroform phase was collected, evaporated, and extracted. Higher methanol phase was then eluted with 300 mL diethyl ether (Merck, India) 3 times. Finally, ether phase was collected, evaporated, and extracted. It is suggested that semi polar components could be separated in these two chloroform and ether phases. The extracts were kept in refrigerator until used for injection in mice [52].

Experiments and Groups (A) Toxicity Assay of A. sieberi Herbal Extract in Naïve
Animals. In vivo toxicity was assessed by using herbal extract on naïve NMRI male mice. Animals were divided into four groups (n = 5 mice/group), including Group 1 (naïve), Group 2 (low dose), Group 3 (average dose), and Group 4 (high dose). According to several publications of this laboratory [51][52][53][54], in a blind experiment with no previous findings, three different concentrations ranging from 1 and 100 mg/mL can be used. A sample of herbal extract was suspended in ethanol and normal saline (1 : 9), then three different concentrations (low, average, and high doses) of herbal extracts including 1, 10, and 100 mg/mL were tested in vivo for their toxicity as test animals and a control group which was injected with drug vehicle. The parasite specificity of action was blood stage ring forms. Entire animals in all groups were injected with 200 µL of related solutions subcutaneously (sc) once a day for 5 days.

(B) Antimalarial Effects of Herbal Extract on P. berghei Infected
Mice. Following toxicity assay, the highest dose with the lowest toxicity of herbal extract (100 mg/mL concentration) was selected to apply for its antimalarial activity on male NMRI mice infected with P. berghei. Animals were divided into two groups (n = 10 mice/group), including control and test; both groups were infected with murine malaria parasite, P. berghei. Drug vehicle and herbal extract were injected sc into control and test groups, respectively, once a day with 200 µL of solutions for the period of 10 days.

(C) Antimalarial Effects of Ether and Chloroform Extracts on P. berghei Infected Mice.
The antimalarial efficacy of ether and chloroform extracts was investigated on murine malaria P. berghei infected NMRI mice. Animals were divided into four groups (n = 5 mice/group), including ether extract control and test, chloroform extract control and test groups. Drug vehicle and extracts were injected sc into control and test groups, respectively, once a day with 200 µL of solutions for the period of 14 days. Percentage of parasitaemia and pathophysiology were also evaluated.

Statistical Analysis.
Values are presented as the mean ± SEM for groups of n samples. The significance of differences was determined by analysis of variances (ANOVA) and Student's t-test using Graph Pad Prism Software (Graph Pad, San Diego, CA, USA).

Results
Results of this experiment were classified in the following three steps including (A), (B), and (C).   Spleen weight (g)

Control Test
Control Test (h) Figure 4: Pathophysiological alterations induced by A. sieberi ether and chloroform extracts in malarial animals. Pathophysiological alterations including body weight, survival rate, and hepato/splenomegaly were evaluated as indices of toxicity by ether and chloroform extracts of A. sieberi in control and malarial groups (test, A. sieberi ether and chloroform extracts; control, drug vehicle, n = 10 mice/day/group, Student's t-test, * P < 0.05, * * P < 0.01, * * * P < 0.001).
(B) Antimalarial Effects of Total Herbal Extract on P. berghei Infected Mice. No side effects on pathophysiology were represented by total extract in malarial mice ( Figure 2). The results indicated significant effects of total extract on reducing parasitaemia in test group in comparison with control group (Figure 3). extracts on malaria by high reduction degree of parasitaemia ( Figure 5).

Discussion
Although various species of the genus Artemisia were used for their pharmacological, antimicrobial, and antioxidant activity, only few species of this genus including A. scoparia, A. sieberi, and A. aucheri are widely distributed in desert area of Iran. This investigation is the first report on application of A. sieberi extracts on the treatment of murine malaria.
The results of this assessment showed no toxicity even with high concentration of herbal extract, which confirms its minimal side effects. In spite of less efficacy of crude extract of herb, ether and chloroform extracts were isolated from A. sieberi and were successfully tested in P. berghei murine malaria. Although a significant reduction was observed in the percentage of parasitaemia, no pathophysiological alterations were indicated in host hepato/splenomegaly and in body weight. The microscopic examination of Giemsastained slides showed a virtual absence of blood stage of the murine malaria treated with this herbal extracts. These observations suggest that the active constituents in the extract may be cytotoxic for P. berghei, thereby inhibiting their development to the erythrocytic stage.
In authors' previous publications [52][53][54][55][56], antimalarial effects of different Iranian flora of Artemisia herbal extracts including A. turanica A. khorassanica, A. diffusa, A. absinthium, and their effective agent (Tehranolide) against malaria and/or leishmania were successfully evaluated. The route of inoculation is important factor to determine herbal efficacy. Although subcutaneous injection was used in this study, other routes may be recommended for future studies. In addition to authors' previous publications [52][53][54][55][56], data of this study specifically indicated the inhibitory effects of the A. sieberi ether and chloroform extracts on the developmental stages of P. berghei by decreasing parasitaemia. The microscopic examination of Giemsa-stained slides showed a virtual absence of blood stage of the murine malaria treated with these herbal extracts. These observations suggest that the active constituents in the extract may be cytotoxic for P. berghei, thereby inhibiting their development to the erythrocytic stage. Although this study confirmed antimalarial effects of A. sieberi extracts against murine malaria in vivo during infection; however, there are more efficacies on pathophysiological symptoms by this medication. These observations provide the basis for the traditional use of this herb in treatments of malaria disease.
Conclusively, the A. sieberi extract had antimalarial effects against murine malaria in vivo. Moreover, some efficacies are indicated on reducing pathophysiological symptoms by this medication. However, there is requirement to find the major component of this herbal extract by further studies. More investigations are required on different Plasmodia and animal hosts to clarify details of antimalarial effects of A. sieberi and analysis of its natural components.