Oxidative Medicine and Cellular Longevity
Volume 2016 (2016), Article ID 8591286, 11 pages
http://dx.doi.org/10.1155/2016/8591286

Research Article

Chemical Characterization, Free Radical Scavenging, and Cellular Antioxidant and Anti-Inflammatory Properties of a Stilbenoid-Rich Root Extract of Vitis vinifera

Tuba Esatbeyoglu,¹ Philipp Ewald,² Yoshiaki Yasui,³ Haruka Yokokawa,³ Anika E. Wagner,¹ Seiichi Matsugo,3,4 Peter Winterhalter,² and Gerald Rimbach¹

¹Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany
²Institute of Food Chemistry, TU Braunschweig, Schleinitzstrasse 20, 38106 Braunschweig, Germany
³Division of Natural System, Graduate School of Natural Science and Technology, Kanazawa University, Kakumamachi, Kanazawa 920-1192, Japan
⁴School of Natural System, College of Science and Technology, Kanazawa University, Kakumamachi, Kanazawa 920-1192, Japan

Received 23 July 2015; Revised 8 September 2015; Accepted 10 September 2015

Academic Editor: Ahmed Abdel Moneim

Copyright © 2016 Tuba Esatbeyoglu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Dietary stilbenoids are receiving increasing attention due to their potential health benefits. However, most studies concerning the bioactivity of stilbenoids were conducted with pure compounds, for example, resveratrol. The aim of this study was to characterize a complex root extract of Vitis vinifera in terms of its free radical scavenging and cellular antioxidant and anti-inflammatory properties. HPLC-ESI-MS/MS analyses of the root extract of Vitis vinifera identified seven stilbenoids including two monomeric (resveratrol and piceatannol), two dimeric (trans-ɛ-viniferin and ampelopsin A), one trimeric (miyabenol C), and two tetrameric (r-2-viniferin = vitisin A and r-viniferin = vitisin B) compounds which may mediate its biological activity. Electron spin resonance and spin trapping experiments indicate that the root extract scavenged 2,2-diphenyl-1-picrylhydrazyl, hydroxyl, galvinoxyl, and superoxide free radicals. On a cellular level it was observed that the root extract of Vitis vinifera protects against hydrogen peroxide-induced DNA damage and induces Nrf2 and its target genes heme oxygenase-1 and γ-glutamylcysteine synthetase. Furthermore, the root extract could induce the antiatherogenic hepatic enzyme paraoxonase 1 and downregulate proinflammatory gene expression (interleukin 1β, inducible nitric oxide synthase) in macrophages. Collectively our data suggest that the root extract of Vitis vinifera exhibits free radical scavenging as well as cellular antioxidant and anti-inflammatory properties.

1. Introduction

Stilbenoids are secondary plant metabolites which are mainly present in Vitis vinifera L. species, the latter belonging to the plant family Vitaceae [1]. Vitis vinifera derived stilbenoids exist as monomers, that is, trans-resveratrol or piceatannol, and oligomers [1] and mostly occur in the plant kingdom as trans-isomers (E) [1]. The stilbene aglycone consists of two aromatic rings linked by an ethylene bridge [1]. Beside Vitis vinifera, dietary sources of resveratrol (trans-3,5,4′-trihydroxystilbene) and its oligomers are tea, peanuts, and pistachios [1–4]. Table 1 summarizes the occurrence of different stilbenoids in cell suspension culture, berries, stems, leaves, roots, and wine of Vitis vinifera according to Pawlus et al. [5]. Stilbenoids exhibit antimicrobial properties and as phytoalexins they play an important role in plants defending pathogens [5].

Table 1: Stilbenoids in cell suspension culture, berries, stems, leaves, roots, and wine of Vitis vinifera according to Pawlus et al. [5].

Plant part
Stilbenoids

Cell suspension culture
Monomer: E-/Z-Astringin, E-/Z-Piceid, E-/Z-Resveratrol, E-/Z-Resveratrol-3,4′-O-β-diglucoside, E-/Z-Resveratrol-3,5-O-β-diglucoside, Z-Resveratrol-3,5,4′-O-β-triglucoside, E-/Z-Resveratroloside 
Dimer: Pallidol, E-δ-Viniferin, E-δ-Viniferin-11-O-β-D-glucopyranoside, E-δ-Viniferin-11′-O-β-D-glucopyranoside

Berries
Monomer: E-Piceatannol, E-/Z-Piceid, E-Pterostilbene, E-Resveratrol

Stems
Monomer: E-Piceatannol, E-/Z-Piceid, E-/Z-Resveratrol, E-Resveratrol-2-C-glucoside 
Dimer: (+)-Ampelopsin A and F, (−)-Malibatol A, Pallidol, Scirpusin A, Viniferifuran, (+)-E-ε-Viniferin, E-ε-Viniferin 
Trimer: E-trans-Miyabenol C, (+)-Viniferol D 
Tetramer: Hopeaphenol, Isohopeaphenol, (+)-Viniferol A, B and C, (+)-Vitisifuran A and B, Vitisin A, E-Vitisin B and C

Leaves
Monomer: E-Piceid, E-Pterostilbene, E-/Z-Resveratrol 
Dimer: Ampelopsin D, Pallidol, Quadrangularin A, E-δ-Viniferin, Z-ε-Viniferin, (+)-E-ε-Viniferin, E-ε-Viniferin, E-/Z-ω-Viniferin 
Trimer: E-/Z-trans-Miyabenol C, E-cis-Miyabenol C, α-Viniferin 
Tetramer: Ampelopsin H, Hopeaphenol, Isohopeaphenol, Vaticanol C isomer

Roots
Dimer: (+)-Viniferether A and B, E-ε-Viniferin 
Trimer: Gnetin H 
Tetramer: Hopeaphenol, (+)-Viniferol E, E-Vitisin B

Wine
Monomer: E-/Z-Astringin, E-Piceatannol, E-/Z-Piceid, E-/Z-Resveratrol, E-Resveratrol-2-C-glucoside, 2,4,6-Trihydroxyphenanthrene-2-O-glucoside 
Dimer: Pallidol, Pallidol-3,3′′-diglucoside, Pallidol-3-O-glucoside, Parthenocissin A, E-δ-Viniferin, Z-ε-Viniferin, E-ε-Viniferin, E-/Z-ε-Viniferin-diglucoside 
Tetramer: Hopeaphenol

Not distinguished between red wine and white wine.

Up to now, the majority of studies concerning stilbenoids were conducted with resveratrol as a purified standard compound. However, studies in which a complex stilbene extract of Vitis vinifera was applied are scarce. The use of an extract may lead to synergistic effects of the various stilbenoids as far as their bioactivity is concerned. Stilbenoids are known to exhibit potential health benefits, that are, antioxidant [3, 6], anti-inflammatory [7, 8], anticancerogenic [9], antiatherogenic [10], antiviral [11], and neuroprotective properties [12].

In the current study, we investigated potential free radical scavenging and cellular antioxidant and anti-inflammatory activities of the root of Vitis vinifera, which may be highly enriched with various stilbenoids. A standardized ethanol extract of the root of Vitis vinifera purified with ethyl acetate/n-hexane was applied for all studies. The qualitative and quantitative stilbenoid composition was analyzed by HPLC-ESI-MS/MS and HPLC-PDA.

Plant bioactives may prevent the oxidation of lipids, proteins, and DNA either directly by free radical scavenging or indirectly by induction of endogenous antioxidant defense mechanisms. Free radical scavenging activity was monitored by ESR spectroscopy and as spin trapping and the prevention of DNA damage was determined by the Comet assay.

The redox sensitive transcription factor nuclear factor erythroid 2-related factor-2 (Nrf2) partly regulates the expression of genes encoding antioxidant enzymes. Nrf2 is bound in the cytoplasm to its inhibitor Keap1 (Kelch-like ECH-associated protein 1). When Nrf2 is activated by electrophiles, it is released from its cytosolic protein Keap1 and binds to the antioxidant response element of the DNA in the nucleus thereby regulating the transcription of target genes including γ-glutamylcysteine synthetase (γGCS) and heme oxygenase-1 (HO-1) [13]. Nrf2 transactivation and its target genes HO-1 and γGCS were determined by a reporter gene assay, real-time PCR, and Western blotting, respectively. Paraoxonase 1 (PON1) is a high-density lipoprotein (HDL) associated enzyme which is primarily synthesized in the liver [14]. PON1 prevents low-density lipoproteins (LDL) from oxidation and thereby mediats antiatherogenic effects [14]. PON1 transactivation was measured by a reporter gene assay. Biomarkers of inflammation including interleukin 1β (IL-1β) and inducible nitric oxide synthase (iNOS) were determined in cultured cells by real-time PCR.

2. Materials and Methods

2.1. Chemicals

Methanol, HPLC grade was purchased from VWR (Leuven, Belgium) and methanol, LC-MS grade was purchased from Fisher Chemical (Loughborough, UK). Acetic acid, HPLC grade was obtained from AppliChem (Darmstadt, Germany). Doubly deionized water using a Nanopure resin (Nanopure, Barnstead) was used for high-performance liquid chromatography (HPLC) analyses.

Sodium dihydrogen phosphate dihydrate and galvinoxyl radical were purchased from Wako Chemicals (Osaka, Japan). Disodium hydrogen phosphate, hypoxanthine, xanthine oxidase, and ethanol were obtained from Nacalai Tesque (Kyoto, Japan). Hydrogen peroxide (35%) was purchased from Tokyo Chemical Industry (TCI, Tokyo, Japan). 5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) was purchased from Sigma-Aldrich (St. Louis, MO, USA (Tokyo branch)). Ultrapure water was prepared by PWE-500 (Advantec, Tokyo, Japan) for electron spin resonance spectroscopy (ESR).

Dulbecco’s modified Eagle’s medium high glucose (4.5 g/L) (with sodium pyruvate and L-glutamine), Dulbecco’s modified Eagle’s medium high glucose (4.5 g/L) (with sodium pyruvate), Dulbecco’s modified Eagle’s medium high glucose (4.5 g/L), fetal bovine serum, L-glutamine 200 mM (100x), penicillin/streptomycin (100x; 10,000 U/mL penicillin; 10 mg/mL streptomycin), G-418 sulfate (50 mg/mL), fetal bovine serum, and Dulbecco’s phosphate-buffered saline (DPBS) without Ca and Mg were obtained from PAA (Cölbe, Germany). Neutral red and glacial acetic acid (99–100%) were purchased from Carl Roth (Karlsruhe, Germany) and ethanol (absolute) was purchased from Merck (Darmstadt, Germany). Resveratrol and dimethyl sulfoxide were obtained from Sigma (Steinheim, Germany). Lipopolysaccharide (LPS) from Salmonella enterica serotype Enteritidis was obtained from Sigma. Dual-luciferase reporter assay system, phRL-TK, and passive lysis buffer were purchased from Promega (Mannheim, Germany). JetPEI and peqGOLD TriFast were obtained from Peqlab Biotechnologie GmbH (Erlangen, Germany). Primers for heme oxygenase-1 (HO-1), γ-glutamylcysteine synthetase (γGCS), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were ordered from Eurofins Genomics (Ebersberg, Germany). SensiMix one-step kit was obtained from Bioline (Luckenwalde, Germany). Heme oxygenase-1 antibody was purchased from Stressgen (Michigan, USA), immun-star goat anti-rabbit (GAR)-HRP conjugate secondary antibody was purchased from Bio-Rad (Munich, Germany), and Pierce ECL Western blotting substrate, spectra multicolor broad range protein ladder, and Pierce BCA protein assay kit were purchased from Thermo Scientific (Rockford, USA). GAPDH antibody and donkey anti-goat IgG HRP secondary antibody were obtained from Santa Cruz Biotechnology (Heidelberg, Germany).

Vitisin grapevine root extract (Vitis vinifera cultivated from vines of the area Bordeaux) was kindly provided by Wolfgang Loersch (Breko, Bremen, Germany).

2.2. HPLC-Photodiode Array Detector (HPLC-PDA)

HPLC analysis was done according to Macke [15]. HPLC system from Jasco (Gro-Umstadt, Germany) was used which consisted of a pump (PU-2080 Plus, Intelligent HPLC Pump), degasser (DG-2080-53, 3-Line Degasser), ternary gradient unit (LG-2080-02), autosampler (Intelligent Sampler AS-2057 Plus), and PDA (MD-2010 Plus). The separation was done on a Kromasil 100-5 C18 5 μm (250 mm × 4.6 mm i.d.) column (Eka Chemicals AB, Bohus, Sweden) protected with a guard column of the same material (4 mm × 4 mm). The mobile phase consisted of 1% aqueous acetic acid (A) and methanol (B). The following gradient was used for separation: 0 min 20% B, 5 min 30% B, 15 min 30% B, 18 min 37% B, 29 min 37% B, 35 min 50% B, 57 min 50% B, 58 min 100% B, 71 min 100% B, 72 min 20% B, and 75 min 20% B. The flow rate was set at 0.8 mL/min and the injection volume was 20 µL. HPLC chromatograms were recorded at  nm. The root extract of Vitis vinifera was dissolved in methanol/water (80/20, v/v). Monomeric stilbenoids in the root extract of Vitis vinifera were quantified as trans-resveratrol equivalents and oligostilbenoids as trans-ɛ-viniferin equivalents. The measurements were repeated five times.

2.3. HPLC-Electrospray Ionization-Tandem Mass Spectrometry (HPLC-ESI-MS/MS)

A HPLC analysis was performed according to Macke [15]. HPLC system from Agilent Technologies (Waldbronn, Germany) composed of a pump (1100 Series, BinPump G1312A), autosampler (1200 Series), PDA (1100 Series, DAD G1315B), and mass spectrometer (HCT Ultra; Bruker Daltonics, Bremen, Germany) was used. The ESI conditions were as follows: ion polarity: negative, scan range: 100–3000 m/z, dry gas temperature: 330°C, dry gas flow: 10 L/min, nebulizer pressure: 50 psi, capillary voltage: 3500 V, capillary exit: −3500 V, and end plate: −500 V. HPLC separation was achieved on a Luna 3u C18 100 A 3 μm (150 mm × 2.0 mm i.d.) column protected with a guard column of the same material (4 mm × 4 mm) (Phenomenex, Aschaffenburg, Germany). Mobile phases and gradient elution were as described above. The flow rate was set at 0.2 mL/min and the injection volume at 5 µL. HPLC chromatograms were recorded at  nm.

2.4. Free Radical Scavenging Activity Measured by Electron Spin Resonance Spectroscopy (ESR)

ESR and spin trapping measurements were conducted according to Esatbeyoglu et al. [16] using a JEOL JES-FR30EX free radical monitor (JEOL Ltd., Akishima, Japan). The amplitude was set at 200 for DPPH radicals, 250 for galvinoxyl, 400 for hydroxyl radicals, and 500 for superoxide radicals.

2.4.1. DPPH and Galvinoxyl Radical Scavenging Experiments

To a reaction mixture containing 360 µL distilled water, 500 µL ethanol, 100 µL 1.875 mM DPPH (in ethanol), or 100 µL 375 µM galvinoxyl radical (in ethanol), 40 µL of the root extract of Vitis vinifera (1.3, 10, 13, 40, 100, and 130 mg/mL; in case of galvinoxyl radical, 1, 1.33, 2, 4, 10, and 1000 mg/mL) was added and stirred for a few seconds. After incubating the solution for 10 min (3 h for galvinoxyl), ESR spectra were recorded.

2.4.2. Hydroxyl Radical Scavenging Experiments

To the reaction mixture of 25 µL 200 mM DMPO, 20 µL 50 mM hydrogen peroxide, 35 µL distilled water, and 10 µL 0.5 mM ferric chloride, 10 µL of the root extract of Vitis vinifera (1, 1.25, 1.67, 2.5, 5, and 10 mg/mL) was added. The reaction mixture was stirred, vortexed, and put on ice for 10 sec.

2.4.3. Superoxide Radical Scavenging Experiments

To the reaction mixture of 30 µL 2 mM hypoxanthine, 30 µL 4 M DMPO, 26 µL 200 mM DPBS buffer solution (pH 7.4), and 4 µL 1 U/mL xanthine oxidase, 10 µL of the root extract of Vitis vinifera (2, 10, 12.5, 25, 50, and 1000 mg/mL) was added. The combined reaction mixture was incubated at 30°C for 1 min.

2.5. Cell Lines

The detailed cell culture conditions regarding the human liver hepatoma cell line Huh7, stably transfected PON1-Huh7 cells, and human colonic adenocarcinoma cell line HT-29 are described by Esatbeyoglu et al. [16].

Murine RAW264.7 macrophages (obtained from the Institute of Applied Cell Culture, Munich, Germany) were cultured in Dulbecco’s modified Eagle’s medium high glucose (4.5 g/L) containing sodium pyruvate and L-glutamine supplemented with 10% (v/v) fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin and grown in a humidified atmosphere of 5% CO₂ at 37°C.

For all cell culture studies, 100 mg/mL stock solutions of the root extract of Vitis vinifera in ethanol and resveratrol in DMSO were prepared and stored at −80°C until further use. LPS from Salmonella enterica serotype Enteritidis (Sigma) was dissolved in DPBS to a stock solution of 1 mg/mL and stored at −20°C until further use.

2.6. Cytotoxicity (Neutral Red Assay)

Cell viability was determined using the colorimetric neutral red assay [17]. PON1-Huh7, Huh7, HT-29, and RAW264.7 cells (0.15 × 10⁶ cells/well, 0.15 × 10⁶ cells/well, 0.4 × 10⁶ cells/well, and 0.08 × 10⁶ cells/well) were seeded in a 24-well plate for 24 h. The cells were treated with the root extract of Vitis vinifera at various concentrations (PON1-Huh7 and Huh7 1–100 μg/mL; RAW264.7 1–50 µg/mL) for 24 h (PON1-Huh7 for 48 h).

2.7. Oxidative DNA Damage (Comet Assay)

HT-29 cells were treated with 50 µg/mL root extract of Vitis vinifera and 50 µM resveratrol as positive control for 14 h at 37°C. Subsequently, cells were treated with 25 µM H₂O₂ in DPBS for 15 min to induce DNA damage. Oxidative DNA damage in HT-29 cells was measured using the Comet assay as described earlier [16].

2.8. Nrf2 Transactivation (Dual-Luciferase Reporter Gene Assay)

Transient transfection and luciferase reporter gene assay for measuring Nrf2 transactivation were conducted as described elsewhere [16].

Transiently transfected Huh7 cells were incubated with the root extract of Vitis vinifera (1, 5, 10, 25, and 50 µg/mL) and 25 µM resveratrol was used as a positive control.

2.9. Determination of Nrf2 Target Genes Heme Oxygenase-1 (HO-1) and γ-Glutamylcysteine Synthetase (γGCS) (RNA Isolation and Real-Time PCR)

Human Huh7 liver cells were seeded in a 6-well plate at a density of 0.9 × 10⁶ cells/well for 24 h. Subsequently, cells were treated with 1, 10, 25, and 50 µg/mL root extract of Vitis vinifera for 6 h. Cells were washed with DPBS and RNA was isolated using peqGOLD TriFast via phenol-chloroform extraction according to manufacturer’s description.

Primers for genes of human origin were designed by Primer3 software: HO-1, F: 5′-CCAGGCAGAGAATGCTGAGT-3′, R: 5′-GTAGACAGGGGCGAAGACTG-3′; γGCS, F: 5′-TTTGGTCAGGGAGTTTCCAG-3′, R: 5′-TGAACAGGCCATGTCAACTG-3′; GAPDH, F: 5′-CAATGACCCCTTCATTGACC-3′, R: 5′-GATCTCGCTCCTGGAAGATG-3′. All primers were ordered from Eurofins Genomics (Ebersberg, Germany).

SensiMix one-step kit (Quantace, Berlin, Germany) was used for real-time PCR. Human GAPDH was used as housekeeping gene.

2.10. Inhibition of LPS-Mediated Interleukin-1β (IL-1β) and Inducible Nitric Oxide Synthase (iNOS) (RNA Isolation and Real-Time PCR)

RAW264.7 macrophages were seeded in 12-well plates at a density of 0.2 × 10⁶ cells/well for 24 h. Afterwards, cells were treated with 20 µg/mL of the root extract of Vitis vinifera for 24 h. LPS (10 ng/mL) was added to the cells for 4 h. RNA was isolated by peqGOLD TriFast according to manufacturer’s protocol. Remaining DNA was lysed using DNAse according to manufacturer’s instructions (New England Biolabs, Ipswich, USA).

Primers for murine genes were designed by Primer3 software and ordered from Eurofins Genomics (Ebersberg, Germany): Interleukin-1β (IL-1β), F: 5′-CAGCTATGGCAACTGTTCCT-3′, R: 5′-CTGGATGCTCTCATCAGGAC-3′; inducible nitric oxide synthase (iNOS), F: 5′-GGCAGCCTGTGAGACCTTTG-3′, R: 5′-GCATTGGAAGTGAAGCGTTTC-3′; GAPDH, F: 5′-CCGCATCTTCTTGTGCAGT-3′, R: 5′-GGCAACAATCTCCACTTTGC-3′.

SensiMix one-step kit (Quantace, Berlin, Germany) was used for real-time PCR. Gene expression was normalized to the housekeeping gene GAPDH.

2.11. HO-1 Protein Levels (Western Blot Analysis)

Whole cell extracts, total protein determination, and Western blot analysis were performed according to Wagner et al. [18] and Esatbeyoglu et al. [16].

2.12. PON1 Transactivation (Luciferase Reporter Gene Assay)

Luciferase reporter gene assay for measuring PON1 transactivation was described in Schrader et al. [19]. PON1-Huh7 cells were seeded at a density of 0.15 × 10⁶ cells/well in 24-well plates and incubated with 1, 2.5, 5, 15, and 25 µg/mL of the root extract of Vitis vinifera and 25 µmol/L resveratrol.

2.13. Statistical Analyses

Data obtained from cell culture experiments were expressed as means + standard error of the mean (SEM) or standard deviation (SD) of three independent experiments and compared to untreated cells (control) or LPS-stimulated control cells. HPLC analyses of stilbenes were expressed as means + standard deviation of five injections. Statistical analysis was performed by PASW Statistics Software Version 18 (IBM, Chicago, Illinois, USA). Data were tested for normality of distribution (Shapiro-Wilk test). Significant differences between groups were analyzed by Student’s -test. In case of not normally distributed data the non-parametric Mann-Whitney test was applied. Significance was accepted at .

3. Results

3.1. Characterization and Quantification of the Vitis vinifera Root Extract

The grapevine root extract derived from Vitis vinifera was analyzed by HPLC-ESI-MS/MS and quantified by HPLC-PDA. HPLC-ESI-MS/MS analyses were performed using electrospray ionization operated in negative ion mode. Seven stilbenoids including two monomeric (resveratrol and piceatannol), two dimeric (trans-ɛ-viniferin and ampelopsin A), one trimeric (miyabenol C), and two tetrameric (r-2-viniferin = vitisin A and r-viniferin = vitisin B) were detected in the root extract of Vitis vinifera. Chemical structures of all detected compounds are given in Figure 1. A representative HPLC chromatogram at  nm is shown in Figure 2. The main compounds in the root extract of Vitis vinifera were the dimer trans-ɛ-viniferin (125.1 g/kg), a dehydrodimer of resveratrol, and the tetramer r-2-viniferin (87.1 g/kg), composed of two resveratrol dimers (+)-ɛ-viniferin and ampelopsin B, followed by the monomer resveratrol (46.3 g/kg) (Table 2). The monomer piceatannol, the dimer ampelopsin A, the trimer miyabenol C, and the tetramer r-viniferin were present in considerably lower amounts (~4–16 g/kg) (Table 2).

Table 2: Quantification of monomeric stilbenoids in the root extract of Vitis vinifera as trans-resveratrol equivalents and oligostilbenoids as trans-ε-viniferin equivalents by HPLC-PDA at λ = 280 nm ().

Peak
Compound
Retention time () 
min
Molecular ion [M−H]⁻  m/z
Fragment ions m/z
Content [g/kg]
SD [g/kg]

1
Ampelopsin A
16.2
469
451, 363
15.6
0.41

2
Piceatannol
22.3
243
225, 201, 181, 175, 159
4.20
0.34

3
r-Viniferin
(Vitisin B)
25.5
905
887, 799, 705, 675, 545, 451, 359
11.1
0.35

4
Resveratrol
36.2
227
212, 185, 159, 141, 107
46.3
0.85

5
r-2-Viniferin
(Vitisin A)
37.3
905
887, 811, 705, 675, 545, 451, 359
87.1
1.31

6
Miyabenol C
40.4
679
661, 637, 585, 479, 451, 345
12.7
1.30

7
trans-ε-Viniferin
42.8
453
435, 411, 359, 347
125.1
1.23

Figure 1: Chemical structures of monomeric and oligomeric stilbenoids of the root extract of Vitis vinifera.

Figure 2: HPLC chromatogram of the root extract of Vitis vinifera at  nm. For peak numbers, compare Table 2.

3.2. Radical Scavenging Activity of the Root Extract of Vitis vinifera

The free radical scavenging activity of the root extract of Vitis vinifera was determined by ESR and spin trapping analysis. The root extract of Vitis vinifera exhibited relatively potent free radical scavenging activity in terms of DPPH, hydroxyl, and galvinoxyl radicals and scavenged these free radicals in a dose-dependent manner (Figures 3(a)–3(c)). Superoxide radicals were scavenged at higher concentrations of the root extract (Figure 3(d)).

(a)

(b)

(c)

(d)

Figure 3: The scavenging effect of the root extract of Vitis vinifera on DPPH (a), hydroxyl (b), galvinoxyl (c), and superoxide free radical (d) measured by electron spin resonance spectroscopy (ESR). ESR spectra were recorded three times. Data are means + SD.

3.3. Cytotoxic Effects of the Vitis vinifera Root Extract in Huh7, PON1-Huh7, HT-29, and RAW264.7 Cells

Cytotoxicity measurements of the root extract of Vitis vinifera at different concentrations were carried out by the neutral red assay. Huh7, PON1-Huh7, HT-29, and RAW264.7 cells were treated with increasing concentrations of the root extract of Vitis vinifera for 24 h (PON1-Huh7 for 48 h). The root extract of Vitis vinifera was not cytotoxic up to a concentration of 50 µg/mL in Huh7, PON1-Huh7, and HT-29 cells and up to 20 µg/mL in RAW264.7 cells (data not shown). The root extract of Vitis vinifera was used in noncytotoxic concentrations in the subsequent cell culture experiments.

3.4. Prevention of Oxidative DNA Damage Induced by Hydrogen Peroxide

Comet assay was used to determine the effects of the root extract of Vitis vinifera counteracting H₂O₂-induced DNA damage in HT-29 cells. Treatment of HT-29 cells with 50 µg/mL of root extract of Vitis vinifera for 14 h resulted in a moderate but significant protection of H₂O₂-induced DNA damage as shown in Figure 4.

(a)

(b)

Figure 4: Effect of 50 µg/mL root extract of Vitis vinifera on H₂O₂-induced DNA damage in HT-29 cells after 14 h of incubation. Following treatment cells were stressed with 25 µM H₂O₂ for 15 min. Resveratrol (Resv., 50 µM) was used as a positive control. DNA damage was measured by the Comet assay. The photographs represent the comet tails (a) and the inhibition of DNA damage is shown as percentage of control damage (damage of control = 100%; (b)). Each bar represents the mean of three independent experiments + SD. indicates significant differences compared to untreated control cells; , Student’s -test.

3.5. Induction of Antioxidant Defense Mechanisms through Nrf2 Transactivation

Treatment of Huh7 cells with the root extract of Vitis vinifera at concentrations of 25 μg/mL and 50 μg/mL significantly () upregulated Nrf2 transactivation. This effect was comparable with the Nrf2 inducing activity of 25 µM resveratrol (Figure 5). Moreover, mRNA and protein levels of the Nrf2 target genes HO-1 and γGCS were analyzed by real-time PCR and Western blotting in human liver Huh7 cells. The root extract of Vitis vinifera (50 μg/mL) significantly induced HO-1 both on the mRNA () (Figure 6(a)) and protein levels (Figure 6(b)). Accordingly, a significant induction of γGCS was observed at 50 μg/mL root extract of Vitis vinifera (; Figure 7).

Figure 5: Effect of the root extract of Vitis vinifera on Nrf2 transactivation in transiently transfected Huh7 liver cells. Resveratrol (Resv., 25 µM) was used as positive control. Data are mean + SEM of at least three experiments performed in triplicate. indicates significant differences compared to control; , Mann-Whitney test.

(a)

(b)

Figure 6: (a) HO-1 mRNA levels in Huh7 liver cells following 6 h of incubation with the root extract of Vitis vinifera compared to untreated control cells. Resveratrol (Resv., 25 µM) was used as positive control. Data are means + SEM of at least three experiments. indicates significant differences compared to untreated control; , Student’s -test, and indicates significant differences compared to untreated control cells; . (b) Western blotting of HO-1 in Huh7 whole cell extracts following 24 h of incubation with the root extract of Vitis vinifera. Resveratrol (Resv., 25 µM) was used as positive control and GAPDH was used as loading control. One representative Western blot out of three is shown.

Figure 7: γGCS mRNA levels in Huh7 liver cells following 6 h of incubation with the root extract of Vitis vinifera compared to untreated control cells. Resveratrol (Resv., 25 µM) was used as positive control. Data are means + SEM of at least three experiments. indicates significant differences compared to untreated control cells; , Student’s -test.

3.6. PON1 Transactivation

Under the conditions investigated, luciferase reporter gene activity of stably transfected PON1-Huh7 cells was significantly () induced by the root extract of Vitis vinifera in a dose-dependent manner (Figure 8).

Figure 8: Effects of the root extract of Vitis vinifera on PON1 transactivation in stably transfected PON1-Huh7 cells. Resveratrol (Resv., 25 µM) was used as positive control. Data are means + SEM of at least three independent experiments performed in triplicate. indicates significant differences compared to control; , Mann-Whitney test.

3.7. Inhibition of Proinflammatory Biomarkers like IL-1β and iNOS due to Vitis vinifera Root Extract

Furthermore, the root extract of Vitis vinifera (20 μg/mL) significantly decreased the NF-κB target genes IL-1β (Figure 9(a)) and iNOS (Figure 9(b)) on the mRNA level in LPS-stimulated murine RAW264.7 macrophages.

(a)

(b)

Figure 9: Effect of the root extract of Vitis vinifera on the inflammatory biomarkers IL-1β (a) and iNOS (b) in murine macrophages. RAW264.7 murine macrophages were incubated with the root extract of Vitis vinifera (20 µM) for 24 h and stimulated with 10 ng/mL lipopolysaccharide (LPS) for 4 h. mRNA levels of IL-1β and iNOS were examined with real-time PCR. Each bar represents the mean (SEM) of at least three independent experiments measured in duplicate. indicates significant differences compared to stimulated control; , Mann-Whitney test (a) and Student’s -test (b).

4. Discussion

Stilbenoids are currently receiving increasing attention due to their potential health benefits [1, 6, 7, 10–12, 20]. In this study, we combined ESR and spin trapping measurements with cellular assays in order to determine the free radical scavenging and antioxidant and anti-inflammatory properties of a root extract of Vitis vinifera.

Our analyses indicate that the root extract of Vitis vinifera contained substantial amounts of dimeric and oligomeric stilbenoids including the dimer trans-ɛ-viniferin and the tetramer r-2-viniferin which may have contributed to its free radical scavenging properties. The free radical scavenging activity of stilbenoids seems to be partly related to proton abstraction as previously reported [21, 22].

Since the root extract contained a portfolio of various stilbenoids, these compounds may interact synergistically thereby exhibiting free radical scavenging and antioxidant activity [23].

On a cellular level, free radicals are inactivated by endogenous antioxidant and stress response mechanisms. We found that root extract of Vitis vinifera exhibited HO-1 and γGCS inducing activity which is most likely due to Nrf2 activation. Oxidized LDL plays a central role in atherogenesis [24]. Stilbenoids, such as resveratrol, have been shown to prevent copper mediated LDL oxidation in vitro through free radical scavenging activity [10]. Alternatively, our data indicate that a stilbenoid-rich extract may prevent LDL oxidation via cell signaling due to PON1 induction. Thus, stilbenoids may exhibit antiatherogenic properties due to both free radical scavenging and induction of antioxidant defense mechanisms. Interestingly, HO-1, γGCS, and PON1 decrease with age [25]. Thus, it is tempting to speculate that our root extract may counteract an aging phenotype which warrants further investigations in appropriate in vivo models such as laboratory rodents. Furthermore, other age-related molecular targets including sirtuins [25, 26] and FOXO [26] as well as autophagy related pathways [27] should be taken into account since they have been reported to be modulated by resveratrol in cultured cells and various model organisms.

Recent studies suggest cross talk between Nrf2 and proinflammatory gene expression. Nrf2 counteracts inflammatory processes by downregulating NF-B [28, 29]. In the present study, the root extract of Vitis vinifera significantly decreased the expression of the NF-B target genes IL-1β and iNOS in murine macrophages suggesting anti-inflammatory activity. These anti-inflammatory properties of the Vitis vinifera root extract and other related plant extracts may be beneficial in pathologies characterized by an overproduction of nitric oxide and inflammatory cytokines [30]. Additionally, other biological properties of the root extract including its effect on platelet aggregation [31], smooth muscle cell proliferation [32], cellular adhesion [33], and vasodilation [34] should be taken into consideration.

5. Summary and Conclusion

In this study, seven stilbenoids including resveratrol, piceatannol, trans-ɛ-viniferin, ampelopsin A, miyabenol C, r-2-viniferin = vitisin A, and r-viniferin = vitisin B were identified in the root extract of Vitis vinifera by HPLC-PDA. The root extract of Vitis vinifera scavenged DPPH, hydroxyl, galvinoxyl, and superoxide free radicals. Accordingly, a protection against hydrogen peroxide-induced DNA damage was observed in cultured cells. Furthermore, Nrf2 and its target genes HO-1 and γ-GCS as well as PON1 were induced by Vitis vinifera root extract. Moreover, the root extract downregulated proinflammatory gene expression including IL-1β and iNOS in cultured macrophages. To sum up, our results suggest free radical scavenging and cellular antioxidant and anti-inflammatory activities of the Vitis vinifera root extract in vitro. However, little is known about the bioavailability, metabolism, and bioactivity of root-derived stilbenoids in vivo. Therefore, future studies should address the question to which extent stilbenoids from the roots of Vitis vinifera are bioavailable and may exhibit potential health benefits in humans.

In addition, both the food industry and the consumer exhibit an increasing demand for natural antioxidants [35]. Thus, further studies are needed to elucidate to which extent the stilbenoid-rich Vitis vinifera root extracts could prevent oxidation processes, such as lipid peroxidation, in the food matrix. Additionally, it needs to be established whether the present Vitis vinifera root extract may be used as a nutraceutical in functional foods.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Acknowledgments

The authors thank V. Schmuck, S. Schweizer, and G. Steinkamp for excellent technical assistance.

References

C. Rivière, A. D. Pawlus, and J.-M. Mérillon, “Natural stilbenoids: distribution in the plant kingdom and chemotaxonomic interest in Vitaceae,” Natural Product Reports, vol. 29, no. 11, pp. 1317–1333, 2012.

R. E. King, J. A. Bomser, and D. B. Min, “Bioactivity of resveratrol,” Comprehensive Reviews in Food Science and Food Safety, vol. 5, no. 3, pp. 65–70, 2006.

A. Carrizzo, M. Forte, A. Damato et al., “Antioxidant effects of resveratrol in cardiovascular, cerebral and metabolic diseases,” Food and Chemical Toxicology, vol. 61, pp. 215–226, 2013.

H. Piotrowska, M. Kucinska, and M. Murias, “Biological activity of piceatannol: leaving the shadow of resveratrol,” Mutation Research: Reviews in Mutation Research, vol. 750, no. 1, pp. 60–82, 2012.

A. D. Pawlus, P. Waffo-Téguo, J. Shaver, and J.-M. Mérillon, “Stilbenoid chemistry from wine and the genus Vitis, a review,” Journal International des Sciences de la Vigne et du Vin, vol. 46, no. 2, pp. 57–111, 2012.

C. Müller, K. Ullmann, A. Wilkens, P. Winterhalter, S. Toyokuni, and P. Steinberg, “Potent antioxidative activity of vineatrol 30 grapevine-shoot extract,” Bioscience, Biotechnology and Biochemistry, vol. 73, no. 8, pp. 1831–1836, 2009.

T. Minagawa, T. Okui, N. Takahashi et al., “Resveratrol suppresses the inflammatory responses of human gingival epithelial cells in a SIRT1 independent manner,” Journal of Periodontal Research, vol. 50, no. 5, pp. 586–593, 2015.

J. Walker, K. Schueller, L.-M. Schaefer, M. Pignitter, L. Esefelder, and V. Somoza, “Resveratrol and its metabolites inhibit pro-inflammatory effects of lipopolysaccharides in U-937 macrophages in plasma-representative concentrations,” Food & Function, vol. 5, no. 1, pp. 74–84, 2014.

S. Fulda, “Resveratrol and derivatives for the prevention and treatment of cancer,” Drug Discovery Today, vol. 15, no. 17-18, pp. 757–765, 2010.

H. Berrougui, G. Grenier, S. Loued, G. Drouin, and A. Khalil, “A new insight into resveratrol as an atheroprotective compound: inhibition of lipid peroxidation and enhancement of cholesterol efflux,” Atherosclerosis, vol. 207, no. 2, pp. 420–427, 2009.

C. Li, J. S. Fang, W. W. Lian, X. C. Pang, A. Liu, and G. Du, “In vitro antiviral effects and 3D QSAR study of resveratrol derivatives as potent inhibitors of influenza H1N1 neuraminidase,” Chemical Biology & Drug Design, vol. 85, no. 4, pp. 427–438, 2015.

T. Ma, M. Tan, J. Yu, and L. Tan, “Resveratrol as a therapeutic agent for Alzheimer's disease,” BioMed Research International, vol. 2014, Article ID 350516, 13 pages, 2014.

S. Singh, S. Vrishni, B. K. Singh, I. Rahman, and P. Kakkar, “Nrf2-ARE stress response mechanism: a control point in oxidative stress-mediated dysfunctions and chronic inflammatory diseases,” Free Radical Research, vol. 44, no. 11, pp. 1267–1288, 2010.

T. Esatbeyoglu, P. Huebbe, I. M. A. Ernst, D. Chin, A. E. Wagner, and G. Rimbach, “Curcumin—from molecule to biological function,” Angewandte Chemie International Edition, vol. 51, no. 22, pp. 5308–5332, 2012.

S. Macke, Aktivitäts-geleitete Isolierung von Resveratrol-Oligomeren aus Weinrebenextrakten [Ph.D. thesis], Technische Universität Braunschweig, Braunschweig, Germany, 2013 (German).

T. Esatbeyoglu, A. E. Wagner, R. Motafakkerazad, Y. Nakajima, S. Matsugo, and G. Rimbach, “Free radical scavenging and antioxidant activity of betanin: electron spin resonance spectroscopy studies and studies in cultured cells,” Food and Chemical Toxicology, vol. 73, pp. 119–126, 2014.

T. Esatbeyoglu, K. Ulbrich, C. Rehberg, S. Rohn, and G. Rimbach, “Thermal stability, antioxidant, and anti-inflammatory activity of curcumin and its degradation product 4-vinyl guaiacol,” Food & Function, vol. 6, no. 3, pp. 887–893, 2015.

A. E. Wagner, I. Ernst, R. Iori, C. Desel, and G. Rimbach, “Sulforaphane but not ascorbigen, indole-3-carbinole and ascorbic acid activates the transcription factor Nrf2 and induces phase-2 and antioxidant enzymes in human keratinocytes in culture,” Experimental Dermatology, vol. 19, no. 2, pp. 137–144, 2010.

C. Schrader, A.-C. Graeser, P. Huebbe, A. E. Wagner, and G. Rimbach, “Allyl isothiocyanate as a potential inducer of paraoxonase-1—studies in cultured hepatocytes and in mice,” IUBMB Life, vol. 64, no. 2, pp. 162–168, 2012.

M. T. Empl, S. Macke, P. Winterhalter et al., “The growth of the canine glioblastoma cell line D-GBM and the canine histiocytic sarcoma cell line DH82 is inhibited by the resveratrol oligomers hopeaphenol and r2-viniferin,” Veterinary and Comparative Oncology, vol. 12, no. 2, pp. 149–159, 2014.

C. Privat, J. P. Telo, V. Bernardes-Genisson, A. Vieira, J.-P. Souchard, and F. Nepveu, “Antioxidant properties of trans-epsilon-viniferin as compared to stilbene derivatives in aqueous and nonaqueous media,” Journal of Agricultural and Food Chemistry, vol. 50, no. 5, pp. 1213–1217, 2002.

S. S. Leonard, C. Xia, B.-H. Jiang et al., “Resveratrol scavenges reactive oxygen species and effects radical-induced cellular responses,” Biochemical and Biophysical Research Communications, vol. 309, no. 4, pp. 1017–1026, 2003.

C. Nopo-Olazabal, J. Hubstenberger, L. Nopo-Olazabal, and F. Medina-Bolivar, “Antioxidant activity of selected stilbenoids and their bioproduction in hairy root cultures of muscadine grape (Vitis rotundifolia Michx.),” Journal of Agricultural and Food Chemistry, vol. 61, no. 48, pp. 11744–11758, 2013.

F. Virgili, R. Ambra, F. Muratori et al., “Effect of oxidized low-density lipoprotein on differential gene expression in primary human endothelial cells,” Antioxidants & Redox Signaling, vol. 5, no. 2, pp. 237–247, 2003.

U. Kilic, O. Gok, U. Erenberk et al., “A remarkable age-related increase in SIRT1 protein expression against oxidative stress in elderly: SIRT1 gene variants and longevity in human,” PLoS ONE, vol. 10, no. 3, Article ID e0117954, 2015.

M. F. Oellerich and M. Potente, “FOXOs and sirtuins in vascular growth, maintenance, and aging,” Circulation Research, vol. 110, no. 9, pp. 1238–1251, 2012.

K. Pallauf and G. Rimbach, “Autophagy, polyphenols and healthy ageing,” Ageing Research Reviews, vol. 12, no. 1, pp. 237–252, 2013.

G.-H. Liu, J. Qu, and X. Shen, “NF-κB/p65 antagonizes Nrf2-ARE pathway by depriving CBP from Nrf2 and facilitating recruitment of HDAC3 to MafK,” Biochimica et Biophysica Acta—Molecular Cell Research, vol. 1783, no. 5, pp. 713–727, 2008.

A. E. Wagner, C. Boesch-Saadatmandi, J. Dose, G. Schultheiss, and G. Rimbach, “Anti-inflammatory potential of allyl-isothiocyanate—role of Nrf2, NF-κB and microRNA-155,” Journal of Cellular and Molecular Medicine, vol. 16, no. 4, pp. 836–843, 2012.

F. Virgili, H. Kobuchi, and L. Packer, “Procyanidins extracted from Pinus maritima (Pycnogenol®): scavengers of free radical species and modulators of nitrogen monoxide metabolism in activated murine RAW 264.7 macrophages,” Free Radical Biology & Medicine, vol. 24, no. 7-8, pp. 1120–1129, 1998.

M. Y. Shen, G. Hsiao, C. L. Liu et al., “Inhibitory mechanisms of resveratrol in platelet activation: pivotal roles of p38 MAPK and NO/cyclic GMP,” British Journal of Haematology, vol. 139, no. 3, pp. 475–485, 2007.

J. W. Kim, S. C. Lim, M. Y. Lee et al., “Inhibition of neointimal formation by trans-resveratrol: role of phosphatidyl inositol 3-kinase-dependent Nrf2 activation in heme oxygenase-1 induction,” Molecular Nutrition & Food Research, vol. 54, no. 10, pp. 1497–1505, 2010.

J. Mikuła-Pietrasik, P. Sosińska, and K. Ksiązek, “Resveratrol inhibits ovarian cancer cell adhesion to peritoneal mesothelium in vitro by modulating the production of α5β1 integrins and hyaluronic acid,” Gynecologic Oncology, vol. 134, no. 3, pp. 624–630, 2014.

K. L. Gordish and W. H. Beierwaltes, “Resveratrol induces acute endothelium-dependent renal vasodilation mediated through nitric oxide and reactive oxygen species scavenging,” American Journal of Physiology: Renal Physiology, vol. 306, no. 5, pp. F542–F550, 2014.

F. Silva, A. Figueiras, E. Gallardo, C. Nerín, and F. C. Domingues, “Strategies to improve the solubility and stability of stilbene antioxidants: a comparative study between cyclodextrins and bile acids,” Food Chemistry, vol. 145, pp. 115–125, 2014.