Protective Role of Antioxidant Huskless Barley Extracts on TNF-α-Induced Endothelial Dysfunction in Human Vascular Endothelial Cells

Oxidative stress and inflammation are considered as two key factors that contribute to the development of atherosclerosis. This study was to investigate the antioxidant capacity of huskless barley and to explore its protective functions through the regulation of the antioxidant defense and inflammatory response in human umbilical vein endothelial cells (HUVEC). The oxygen radical absorbance capacity (ORAC), ferric-reducing antioxidant power (FRAP), and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) scavenging capacity of water and alkali extracts of the polysaccharides from nine huskless barley varieties were investigated in vitro. The antioxidant properties of the alkaline extracts were more pronounced than those of the water extracts. The results from the cell model showed that pretreatment of HUVEC with the water or alkaline extracts of the polysaccharides from the huskless barley cultivars QHH and NLGL decreased the levels of reactive oxygen species (ROS), monocyte chemotactic protein 1 (MCP-1), and vascular cell adhesion molecule 1 (VCAM-1) but increased the level of superoxide dismutase (SOD) and maintained cell viability. Huskless barley polysaccharide extracts exhibited the vasodilatory effect of inhibiting angiotensin-converting enzyme (ACE) production. These discoveries revealed the potent protective functions of barley in oxidative damage and a potential role for barley in preventing chronic inflammation in cardiovascular diseases.


Introduction
The vascular endothelium is the epithelial tissue that lines the blood vessels. This tissue serves as a regulator of the vascular wall function. Changes in its structure and function form the common pathological basis of cardiovascular diseases. Endothelial dysfunction is considered as an important step in the development of atherosclerosis (AS), which is associated with an inflammatory response and the increased interaction of platelets and monocytes with the vessel wall [1,2]. A number of inflammatory mediators are released by the endothelial cells in response to localized injury or trauma. The primary inflammatory mediators are categorized into four main groups: lipids, plasma enzymes, cytokines, and chemokines [3]. Among these mediators, tumor necrosis factor-alpha (TNF-α), a prototypical proinflammatory cytokine that is found in atherosclerotic lesions, can exert direct effects on vascular endothelial cells to induce the expression of adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1) in leukocytes and other inflammatory cells. Accordingly, TNF-α facilitates the progression of atherosclerotic cardiovascular disease [4]. Considerable evidence has shown that antioxidative, anti-inflammatory, and antihypertensive effects may be highly correlated with the inhibition of AS [5,6].
Reactive oxygen species (ROS) may reduce the availability of endothelial nitric oxide (NO). Additionally, the reaction between ROS and NO forms a more reactive peroxide, nitroso peroxide, which further augments endothelial dysfunction [7]. Moreover, increased levels of vascular superoxide inhibit the vascular extracellular superoxide dismutase (ecSOD) activity, which leads to the production of more superoxide, inhibits the function of NO, and causes endothelial damage [8]. Therefore, antioxidants can reduce oxidative stress by directly scavenging ROS, increasing the SOD activity, and regulating NO to improve endothelial function. Together, these processes have the effects of preventing and treating hypertension.
Grains, cereals, vegetables, and fruits are good sources of antioxidants including polyphenols, flavonoids, and polysaccharides [9,10]. As a major cereal crop that is used extensively as an industrial commodity and in fermentation products, barley is a functional cereal that is rich in dietary fiber. Dietary fiber (mainly non-starch polysaccharides) plays an important role in the health benefits associated with whole grain consumption, which has increasingly attracted the interest of scientists [11]. The physiological and functional properties of dietary fiber depend on the food source, extraction method, chemical composition, structure, and particle size [12,13]. Knutsen and Holtekjolen reported that the water extracts of the polysaccharides consisted mostly of beta-glucans whereas the alkaline extracts consisted of arabinoxylans (AX) [14]. The carbohydrate composition in different barley varieties differs considerably; thus, the selection of the variety is also important.
Although it is widely recognized that dietary fiber possesses considerable antioxidant capacity, the antioxidative capacity of barley remains unknown. In the present study, we validated the antioxidant capacity of water and alkali extracts of the polysaccharides from nine huskless barley varieties in vitro by measuring the oxygen radical absorbance capacity (ORAC), ferric-reducing antioxidant power (FRAP), and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) scavenging capacity. Moreover, the protective role of barley in cells was studied. We hypothesized that huskless barley extracts may slow the process of atheroma formation by inhibiting TNF-αinduced cell adhesion through the suppression of superoxide anion production in human umbilical vein endothelial cells (HUVEC). The purpose of this study was to explore the inhibitory effects of the polysaccharide extracts of barley on the vascular inflammatory process in TNF-αinduced HUVEC and to elucidate the mechanism of the antioxidative action. We conducted research on the protection of vascular endothelium by investigating the expression of several molecules in TNF-α-induced HUVEC that had been pretreated with polysaccharide extracts from two representative huskless barley cultivars. Moreover, the results from two extraction methods (water and alkaline) were compared to help understand and analyze the active component in extracts. A human umbilical vein endothelial cell (HUVEC) line was purchased from Zhongqiao Xinzhou Biological Technology Co. Ltd. (Shanghai, China). Dulbecco's phosphatebuffered saline (PBS), M199 medium, 2,2 ′ -azobis(2-methylpropionamide) dihydrochloride (AAPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), and TNF-α were obtained from Sigma Chemical Co. Ltd. (Nanjing, China). Trolox (6-hydroxy-2,5,7,8-tetramethylchromate-2-carboxylic acid) was obtained from Acros Organics (Shanghai, China). A reactive oxygen species (ROS) assay kit was obtained from the Beyotime Institute of Biotechnology (Shanghai, China). Streptomycin and penicillin were purchased from Life Technologies (Shanghai, China). Enzyme-linked immunosorbent assay (ELISA) kits for superoxide dismutase (SOD), monocyte chemotactic protein 1 (MCP-1), vascular cell adhesion molecule 1 (VCAM-1), and human angiotensin-converting enzyme (ACE) were purchased from Boster Biotechnology Inc. (Wuhan, China). All chemicals were analytical grade.

Extractions of Huskless Barley
Polysaccharide. The extraction method was based on the Association of Official Analytical Chemists (AOAC) 2009.01 protocol with minor modifications. Briefly, 10 g of a milled defatted sample, in duplicate, was subjected to sequential enzymatic digestion by heat-stable α-amylase (50 μL, 80°C water bath for 1 h), papain (15 mg, 60°C water bath for 1 h), and glucoamylase (400 μL, 60°C water bath for 1 h) to remove the starch and protein. The slurry was incubated twice with distilled water at 80°C for 3 h. After centrifugation at 3000 rpm for 30 min, the supernatant was combined with the water washings of the residue, and this mixture was precipitated in ethanol (100%, 3 volumes) with continuous stirring. The precipitate was washed with acetone, dried under nitrogen, and labeled as hot water-extracted polysaccharide (WE). To prepare the alkaline extracts of the polysaccharides, the residue was washed twice with NaOH (0.5 mol/L, 40 mL) in a 70°C water bath for 2 h. The alkaline washings were centrifuged at 3000 rpm for 30 min. The mixture was neutralized with 0.5 M HCl. The supernatant was dialyzed with three times its volume of distilled water and precipitated in ethanol (100%, 3 volumes) with continuous stirring. The precipitate was washed with acetone, dried under nitrogen, and labeled as alkaline-extracted polysaccharide (AE).

2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic Acid)
Diammonium Salt-Scavenging Capacity Assay. The ABTS free radical-scavenging activity of each extracted sample was determined according to the method described by Arts et al. [15]. Various levels (4, 2, 1, 0.5, 0.25, and 0.125 g/L) of the samples and a standard solution of Trolox in DMSO were prepared and assayed. The absorbance at 734 nm of the resulting oxidized solution was detected. The results were expressed in terms of the Trolox equivalent antioxidant capacity (TEAC), that is, micrograms of Trolox equivalent per gram of dry weight (mg Trolox/g dried weight (DW)). The half maximal inhibitory concentration (IC 50 , g/L) was also determined.
2.4. Ferric-Reducing Antioxidant Power Assay. The FRAP assay was conducted using a modified version of the method described by Benzie and Strain [16]. Aliquots of various levels (4, 2, 1, 0.5, 0.25, and 0.125 g/L) of the samples were added to the FRAP reagent. The increase in absorbance at 593 nm was measured. Fresh working solutions of FeSO 4 were used for calibration. The antioxidant capacity was calculated from the linear calibration curve and expressed as micromoles of FeSO 4 equivalent per gram of dry weight (μmol FeSO 4 /g DW).
2.5. Oxygen Radical Absorbance Capacity Assay. The radicalscavenging activity was assayed using the improved ORAC method as described previously [17]. The fluorescence decay curves of fluorescein in the presence of WE, AE, and Trolox at various concentrations were generated with MikroWin Microplate Data Reduction 2000 (Mikrotek Laborsysteme GmbH, Overath, Germany). The ORAC value was calculated from the slope of the sample equation dividing the slope of the Trolox curve obtained for the same assay. The final ORAC value is expressed as micromoles of Trolox equivalent per gram of dry weight (μmol Trolox equivalent (TE)/g DW).
2.6. Endothelial Cell Culture and Treatment. Human umbilical vein endothelial cells are considered a model system for studying the oxidative stress and anti-inflammatory and antihypertension activities in the vasculature. The cells were cultured according to a laboratory protocol. After stabilization in a reduced serum medium for 4 h prior to the beginning of the actual experiment, the cells were treated with water extracts of QHH (WE-QHH), alkaline extracts of QHH (AE-QHH), water extracts of NLGN (WE-NLGN), and alkaline extracts of NLGN (AE-NLGN) (10 mg/L) for 18 h in a separate set of experiments followed by the stimulation with TNF-α (10 μg/L). Dimethylsulfoxide (DMSO) was used in the control. At the end of the specified incubation period, the supernatants were collected and stored at −80°C until further analysis of MCP-1 and VCAM-1.

Cell Viability Assay.
Human umbilical vein endothelial cells (20000 cells/well) were cultured according to the treatment above after which 20 mL of the stock solution of MTT (5 mg/mL) was added to each well for 4 h. Finally, the incubation medium was removed, and the formazan crystals were dissolved in 150 μL of DMSO. The MTT reduction was measured as the absorbance at 490 nm using a StatFax-2100 Microplate Reader (Awareness Technology Inc., Palm City, FL). The background absorbance of the control wells was subtracted. The analysis was performed in triplicate. Viability (%) = OD (experiment group)/OD (control) × 100%.

Reactive Oxygen Species Assay.
A DCFH-DA detection kit (Beyotime Institute of Biotechnology, Shanghai, China) was used to assess the ROS level in HUVEC. The cells were seeded in 6-well plates, treated with the various samples, and incubated for 24 h. After washing the cells with the reduced-serum medium, 10 μmol/L DCFH-DA was added to each well, and the cells were incubated at 37°C for 20 min. The cells were then washed thoroughly with reduced-serum medium to remove the DCFH-DA that did not enter the cells. The cells were collected in 1 mL of PBS after dissociation, and the fluorescence was immediately recorded using a LB 941 TriStar Microplate Reader (Berthold Technologies, Bad Wildbad, Germany) using 485 nm excitation and 535 nm emission filters. The total fluorescence intensity of the cells in each well was recorded, and ROS generation was measured as the fold increase over the untreated control.
2.9. Enzyme-Linked Immunosorbent Assay. The levels of SOD, MCP-1, VCAM-1, and ACE in the supernatants were quantified using the solid-phase sandwich ELISA kits. The assay procedures were performed according to the instructions in the kit protocol booklets. The samples were used to conduct a protein assay and were suitably diluted in the standard diluent buffer. The absorbance of the resulting yellow color was measured at 450 nm using a StatFax-2100 Microplate Reader (Awareness Technology Inc., Palm City, FL). The reader was controlled via Hyper Terminal Applet ELISA software.
2.10. Statistical Analysis. The data are presented as the mean values ± standard deviation (SD) and were analyzed using the analysis of variance (ANOVA). The data figures were prepared using GraphPad Prism version 5.02 (GraphPad Software Inc., CA, USA). Duncan's multiple range tests (P < 0 05) were conducted to analyze their differences. A two-way ANOVA was used to analyze the differences among the alkaline extracts and water extracts of the nine barley varieties. The statistical analyses were performed using SPSS 17.0 for Windows (SPSS Inc., Chicago, IL, USA).

Antioxidant Capacity of Huskless Barley Polysaccharide Extracts
In Vitro. Table 1 shows the results for the antioxidant capacity of the water and alkali extractions from the nine huskless barley varieties in vitro, including the ABTS, FRAP, and ORAC data. The IC 50 values for the AEs in the ABTS radical assay ranged from 1.74 to 2.84 g/L with a mean value of 2.12 g/L. The mean values for the TEAC, FRAP, and ORAC assays were 8.73 mg/g DW, 91.95 μmol/g DW, and 381.39 μmol TE/g DW, respectively. The IC 50 values for the WEs in the ABTS radical assay ranged from 7.41 to 13.43 g/L with a mean value of 10.59 g/L. The mean values for the TEAC, FRAP, and ORAC assays were 1.79 mg/g DW, 32.61 μmol/g DW, and 251.06 μmol TE/g DW, respectively. There were significant differences between the alkaline and water extracts in the ABTS, FRAP, and ORAC results (P < 0 001). Each alkaline extract possessed a much higher antioxidant capacity than the water extract for each huskless barley variety ( Figure 1).
The various huskless barley varieties exhibited different antioxidant capacities using the different models. The extracts AE-GBGN and AE-SW had the strongest ABTSscavenging capacity with the highest TEAC values (both 10.61 mg/g DW) and the lowest IC 50 values (both 1.74 g/L). The extracts AE-NLGN, AE-YQ2, and AE-BW80 also had high ABTS-scavenging capacities with values over 9.00 mg/g DW TEAC. The extract WE-QHH showed the lowest ABTS-scavenging capacity, but AE-QHH was not the lowest of the nine AEs. The extract AE-JG had the highest FRAP value (131.1 μmol/g DW), followed by AE-YQ2 (111.1 μmol/g DW), AE-SW (100.0 μmol/g DW), AE-NLGN (97.2 μmol/g DW), and AE-GBGN (92.1 μmol/g DW). Both the AE and WE from BW80 had the best oxygen radical-absorbance capacity. The extract WE-BW80 was also superior to the AEs of most varieties. The ORAC values for AE-BW80 and WE-BW80 were 652.45 and 396.57 μmol TE/g DW, respectively. Moreover, AE-JG and AE-YQ2 had high oxygen radical-absorbance capacities, with ORAC values of over than 500 μmol TE/g DW. The extracts of QHH and NLGL showed moderate antioxidant activities in vitro, and their AEs and WEs were used to further study the mechanism of antioxidative action in the cell model.

Effect of Huskless Barley Polysaccharide Extracts on Cell
Viability. To assess whether the inhibitory effect of barley on the stimulation by TNF-α could be attributed to its effect on cell viability, we examined the cytotoxic effect of water and alkaline extracts of the huskless barley polysaccharides using the MTT assay, which provides rapid and precise results for cellular growth (Figure 2). A concentration of 10 μg/L TNF-α substantially decreased the cell viability of HUVEC from 100% to 22.56% (P < 0 001) whereas the huskless barley extract pretreatments effectively reduced the effect of TNF-α, facilitating cell proliferation and vascular reproduction. The viabilities of the cells treated with WE-QHH, AE-QHH, WE-NLGN, and AE-NLGN were 80.91%, 69.66%, 79.76%, and 70.04%, respectively. The water extracts seemed to be more compatible with cellular growth than the alkaline extracts.

Effects of Huskless Barley Polysaccharide Extracts on SOD
and ROS in Cells. The addition of TNF-α (10 μg/L) greatly decreased the level of SOD in the HUVEC (Figure 3(a)). However, when treated with the dietary fiber extracts from barley, the HUVEC presented higher levels of SOD production. Compared to the HUVEC that were stimulated with TNF-α alone, the SOD production of the barley-treated HUVEC was found to be 2.27, 2.62, 3.01, and 4.14 times higher (P < 0 001). The addition of the extracts from the huskless barley decreased the ROS values in the endothelial cells. The water and alkaline extracts from QHH resulted in 10.5% and 11.2% inhibition of ROS, respectively. The presence of NLGL decreased the ROS production by 24.7% (WE) and 35.2% (AE) (Figure 3(b)). Similar to the antioxidant capacity in vitro, the alkaline extracts seemed to have more pronounced antioxidant activity in cells than the water extracts, and NLGL possessed better antioxidant activity than QHH. Similar to the effects on the MCP-1 protein, the levels of the VCAM-1 adhesion molecule in the cells that were pretreated with WE-QHH, AE-QHH, WE-NLGN, and AE-NLGN decreased by 39%, 48%, 62%, and 74%, respectively, compared with the TNF-α-stimulated group (P < 0 001). The extract of NLGL also possessed better anti-inflammatory activity than that of QHH. The difference between the effects of the water extracts and alkaline extracts on the MCP-1 protein expression was not significant. However, the alkaline extract inhibited the VCAM-1 protein expression by the cells more than the water extract for QHH and NLGL (P < 0 05), which was consistent with their antioxidant capacity.

Effects of Huskless Barley Polysaccharide Extracts on the TNF-α-Induced ACE Protein Expression in Cells.
We investigated the effect on ACE production ( Figure 5) in our study. When treated with TNF-α (10 μg/L), the level of ACE in the endothelial cells increased significantly (P < 0 001). The polysaccharide extracts of huskless barley caused a significant reduction in the TNF-α-induced levels of ACE in the endothelial cells (P < 0 001). The extracts WE-QHH, AE-QHH, WE-NLGN, and AE-NLGN inhibited the ACE levels by 23%, 59%, 67%, and 76%, respectively, compared with the TNF-α-induced group. Similarly, the inhibitory effects of the alkaline ACE extracts were more pronounced than those of the water extracts, and the effects of NLGL were more pronounced than those of QHH.

Discussion
Huskless barley has attracted the attention of researchers and food processors in recent years for its potential health benefits. Huskless barley is recognized as a functional grain because it contains high levels of polysaccharides and phytochemicals [18]. In the present study, nine huskless barley varieties exhibited good antioxidant capacity in vitro using various methods (ABTS, FRAP, and ORAC  [19]. For example, the KVL 301 variety had much lower extractability (76%) of mixedlinkage (1 → 3), (1 → 4)-beta-D-glucan after extrusion than the other varieties (91-98%). The antioxidant properties of the alkaline extracts were more pronounced than those of water extracts in vitro and in cells. The different constituents of the water extracts (beta-glucans) and alkali extracts (arabinoxylans) might contribute to the differences in their bioactivities [14]. Sawicki et al. considered that in addition to the dietary fiber, other ingredients in the outer bran layer, including phenolic acids, alkylresorcinols, lignans, phytosterols, and tocols, may also contribute to health benefit outcomes [11]. Zhu et al. found that the extracts from four varieties of dehulled highland barley (Hordeum vulgare L.) showed excellent antioxidant activities as determined by ORAC and cellular antioxidant activity (CAA) assays; additionally, these varieties showed potent antiproliferative activity towards HepG2 human liver cancer cells. The bound phenolics make a significant contribution to antioxidant and anticancer activities in this model [20]. The alkaline extracts had higher total phenolic and flavonoid content than the water extracts in our tested barley varieties. For example, each gram of WE-QHH, AE-QHH, WE-NLGN, and AE-NLGN contained 1.28, 4.20, 2.89, and 5.14 mg of gallic acid equivalent, respectively. The total phenolic content of the alkaline extracts was nearly two to three times that of the water extracts, which may be another major reason for superior antioxidant activities of the alkaline extracts. The HPLC profiles of the extracts also showed that phenolic compounds of the alkaline extracts were more than those of the water extracts, in which phenolic acids (e.g., gallic acid, chlorogenic acid, 3,5-dicaffeoylqunic acid, and 4,5-dicaffeoylqunic acid) and flavonoids (e.g., rutin and astragalin) were detected (see the supporting document). The antioxidant properties of the dietary fiber from huskless barley bran also showed that the DPPH (1,1-diphenyl-2picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl) radical-scavenging activity and ferric-reducing antioxidant power increased with an increase in the total phenolic content [21]. The nine huskless barley varieties evaluated here exhibited antioxidant capacities in vitro, and two representative barley cultivars, QHH and NLGN, showed antioxidant action as indicated by the increases in SOD and decreases in the ROS in the human umbilical vein endothelial cells. Superoxide dismutase is an enzyme that converts deleterious O 2 into less harmful H 2 O 2 [22]. Superoxide dismutase is highly crucial for oxidative stress tolerance [23] and is recognized as an antioxidative defense against endothelial damage [24,25]. Reactive oxygen species have destructive actions on both proteins and DNA and are therefore regarded as pathogenic, resulting in cellular death and arterial disease [26,27]. Increases in ROS are associated with the accumulation of highly reactive free radicals, which exert deleterious effects. Moreover, overexpression of ROS may cause other side effects such as DNA mutations and genetic instability [28]. Therefore, the capacity of water and alkali extracts of the polysaccharides from huskless barley to increase SOD and to decrease ROS indicates that huskless barley can be used as an agent to alleviate harmful effects and protect the cells from oxidative impairment.
In this study, water and alkaline extracts of the polysaccharides from QHH and NLGN inhibited the vascular inflammatory process in TNF-α-induced endothelial cells by reducing the expression of the MCP-1 and VCAM-1 proteins. Cell adhesion molecule MCP-1 accumulates in large quantities during various inflammatory diseases [29,30]. As a proinflammatory cytokine, TNF-α can induce the expression of chemokines, cytokines, and cell adhesion molecules in vascular endothelial cells. Stimulation of MCP-1 gene expression is associated with oxidation-reduction-sensitive mechanisms [31,32]. Cell adhesion molecule VCAM-1 is also important in the inflammatory responses and plays a significant role in cell adhesion and cell signal transduction [4]. Cybulsky et al. found that an early form of cell lesion was significantly diminished by a low level of VCAM-1, which suggested that VCAM-1 contributed to the initiation of AS [33]. In endothelial cells, this adhesion molecule has been revealed to actively participate in the vital functions of immune surveillance and inflammation and the migration of leukocytes from the blood into tissues [34]. The inhibition of the TNF-α-stimulated MCP-1 and VCAM-1 expression indicates that huskless barley might contribute to antiinflammatory activity.
In addition, WE-QHH, AE-QHH, WE-NLGN, and AE-NLGN exhibited vasodilatory effects by inhibiting the production of ACE. Angiotensin-converting enzyme, a carboxyl-terminal dipeptidyl exopeptidase, indirectly leads to hypertension by causing blood vessels to constrict, which is highly correlated with cardiovascular disease [35]. Its mechanism is the conversion of the decapeptide angiotensin I to the potent vasoconstrictor-octapeptide angiotensin II (Ang II). Several studies have shown that Ang II has crucial proinflammatory actions in the vascular wall, including the production of reactive oxygen species that in turn increase the expression of inflammatory cytokines. The inhibition of ACE reduces the Ang II receptor 1 (AT1) expression in HUVEC, which is thought to be the mechanism by which it decreases adhesion molecule production [36]. Therefore, it is reasonable to conclude that huskless barley possesses the potential to reduce the risk of cardiovascular disease through its antihypertensive effects on the vasculature. In this study, huskless barley extracts exhibited antioxidant capacity. Alkaline-extracted polysaccharide possessed better antioxidant capacity and higher phenolic content than hot water-extracted polysaccharide. Since polysaccharides usually present as complex insoluble bound esters with phenolic compounds [37], phenolics might contribute part of antioxidant function. However, because of a small quantity of phenolics in polysaccharide extracts and the complexity of the natural crude extracts, it is rather difficult to characterize every compound and elucidate the structure. Further phytochemical analysis and the detailed composition of polysaccharide from huskless barley will require identification by LC-MS and NMR in the future. In addition, the huskless barley extracts were found to protect HUVEC against TNFα-induced oxidative stress. However, the mechanism on antioxidant function of huskless barley extracts was unknown. Previous studies reported that dietary antioxidants could regulate oxidative stress in cells by activating Nrf2 (nuclear factor erythroid 2-related factor-2) or its related genes, such as wheat bran feruloyl oligosaccharides increasing SOD, catalase, and glutathione peroxidases via Nrf2 signalling [38]. Whether huskless barley extracts protect HUVEC against TNF-α-induced oxidative stress via Nrf2 signalling or its related genes will require further study in the future.

Conclusions
The results of the present study showed that the nine huskless barley varieties had good antioxidant capacities in vitro, including ABTS-scavenging capacity, ferric-reducing antioxidant power, and oxygen radical-absorbance capacity. The antioxidant properties of the alkaline extracts were more pronounced than those of the water extracts. Two representative huskless barley cultivars, QHH and NLGN, alleviated the negative effects of TNF-α by blocking the overexpression of the levels of several key proteins, MCP-1, VCAM-1, and ACE, in the HUVEC. Moreover, these cultivars increased the level of SOD and maintained the cell viability. Thus, these cultivars exerted antioxidant, anti-inflammatory, and antihypertensive effects on vascular endothelial cells and have a potential as potent agents to prevent cardiovascular diseases. (1,1-Diphenyl-2-picrylhydrazyl radical 2,2diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl) DQ3: DiQing 3 DW:

Conflicts of Interest
There is no conflict of interests regarding the publication of this paper.