Various Terpenoids Derived from Herbal and Dietary Plants Function as PPAR Modulators and Regulate Carbohydrate and Lipid Metabolism

Several herbal plants improve medical conditions. Such plants contain many bioactive phytochemicals. Terpenoids (also called “isoprenoids”) constitute one of the largest families of natural products accounting for more than 40,000 individual compounds of both primary and secondary metabolisms. In particular, terpenoids are contained in many herbal plants, and several terpenoids have been shown to be available for pharmaceutical applications, for example, artemisinin and taxol as malaria and cancer medicines, respectively. Various terpenoids are contained in many plants for not only herbal use but also dietary use. In this paper, we describe several bioactive terpenoids contained in herbal or dietary plants, which can modulate the activities of ligand-dependent transcription factors, namely, peroxisome proliferator-activated receptors (PPARs). Because PPARs are dietary lipid sensors that control energy homeostasis, daily eating of these terpenoids might be useful for the management for obesity-induced metabolic disorders, such as type 2 diabetes, hyperlipidemia, insulin resistance, and cardiovascular diseases.


Diversity of Terpenoids in Nature
Nature relies on an intricate network of biosynthetic pathways to produce a lot of small organic molecules required to support life. Terpenoids (also called "isoprenoids") constitute one of the largest families of natural products accounting for more than 40,000 individual compounds of both primary and secondary metabolisms. Most of them are of plant origin, and hundreds of new structures are reported every year [1][2][3]. All organisms naturally produce some terpenoids as part of primary metabolism, but many produce terpenoids via secondary metabolism.
Isopentyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP) are the universal five-carbon precursors of all terpenoids. After the discovery of the mevalonate (MVA) pathway in yeast and animals, it was assumed that IPP was synthesized from acetyl-CoA via MVA and then isomerized to DMAPP in all eukaryotes and some Gram-positive prokaryotes [2,3] (Figure 1). The origins of archeal terpenoids were unknown until recently, when four of the six enzymes have been identified to be present in sequenced genomes [2]. Recently, a nonstandard MVA pathway involving phosphorylation of isopentenyl phosphate has been discovered in Methanocaldococcus jannaschii [4]. Before 1993, the MVA pathway was the only known source of terpenoids. After isotope-labeling studies by Rohmer et al. [5], it has been shown that there is an alternate pathway to terpenoids that do not originate from acetyl-CoA. The complete pathway has been finally elucidated in 2002 [6]. This alternative MVA-independent pathway has been named the methylerythritol phosphate (MEP) pathway (Figure 1), which has been identified in both bacteria and plants [2,3]. Plants use both pathways although they are compartmentalized: MVA to the cytoplasm and   possibly to mitochondria to provide sterols, the side chain of ubiquinone, and sesquiterpenes (C15), and MEP to plastids providing plastidial terpenoids, for example, isoprene (C5), monoterpenes (C10), diterpenes (C20, including gibberellins and the phytyl tail of tocopherols and chlorophylls), and carotenoids (C40) [3]. Moreover, there is evidence that a certain degree of crosstalk between the MVA and MEP pathways can occur, implying that these pathways are not completely autonomous [7]. Plants have an enormous capacity to synthesize huge amounts of diverse terpenoids, particularly via the combination of the terpenoid biosynthetic route and other secondary metabolic pathways. For instance, tocopherol biosynthesis occurs as a result of combination of the shikimate and isoprenoid pathways, which lead to homogentisic acid phytyl diphosphate, which in combination ultimately lead to the formation of tocopherols (formed by a chromanol head group and a phytyl tail) [8].
In addition to universal physiological, metabolic, and structural functions, many specific terpenoids function in various situations, including communication and defense. Members of the isoprenoid group also include industrially useful polymers (e.g., rubber and chicle) and agrochemicals (e.g., pyrethrins and azadirachtin).
It is known that several herbal plants improve medical conditions. Such plants contain many bioactive phytochemicals. In particular, terpenoids are contained in many herbal plants, and several terpenoids have been shown to be available for pharmaceutical applications, for example, artemisinin and taxol as malaria and cancer medicines, respectively. Various terpenoids are contained in many plants for not only herbal medicine use but also dietary use [9].
In this paper, we describe several bioactive terpenoids ( Figure 2) contained in herbal or dietary plants, which have the potential to ameliorate metabolic disorders via activation of ligand-dependent transcription factors, namely, peroxisome proliferator-activated receptors (PPARs).

Nuclear Receptors.
Members of the nuclear receptor superfamily of ligand-dependent transcription factors play a multitude of essential roles in development, homeostasis, reproduction, and immune functions [10][11][12][13][14]. Ligand binding induces a conformational change in nuclear receptors, releasing corepressors in exchange for coactivators. Ligand-activated complexes recruit basal transcriptional machineries, resulting in an enhanced gene expression. Nuclear receptors include the classical steroid hormone receptors (estrogen, progesterone, androgen, glucocorticoid, and mineralcorticoid receptors); "orphan receptors," which exhibit conserved features of the nuclear receptor family but have not been linked to endogenous ligands; and so-called "adopted orphan receptors," which were initially identified as orphan receptors but were subsequently linked to endogenous ligands. The adopted orphan receptors include the thyroid hormone receptors, retinoic acid receptors, PPARs, and liver X receptors (LXRs). Nearly all members of this family contain a highly conserved DNA-binding domain that mediates sequence-specific recognition of target genes and a C-terminal domain that determines the specific ligandbinding properties of each receptor and mediates ligandregulated transcriptional activation and/or repression [10].
Nuclear receptors are frequent biological targets of active compounds contained in herbal and dietary plants. This is perhaps not surprising, since nuclear receptors evolved to be regulated by lipophilic molecules derived from diet and the environment [15,16]. At least ten of these receptors have been shown to be directly activated by compounds purified from herbal remedies [16]. Some compounds have a complex pharmacology; for example, grapeseed-derived resveratrol is a ligand of estrogen receptors and PPARs [17,18] but has also been suggested to activate sirtuin 1 (SIRT1), an NAD +dependent protein deacetylase enzyme implicated in the biology of aging [19]. Other phytochemicals target multiple nuclear receptors [16].

PPARs.
PPARs are members of the nuclear receptor superfamily, which are activated by fatty acids and their derivatives. PPARs are dietary lipid sensors that regulate lipid and carbohydrate metabolism [20]. In mammals, three subtypes of PPAR, α, δ, and γ, were found [21]. PPARs form heterodimers with retinoid-X-receptors (RXRs) and bind to consensus DNA sites composed of direct repeats (DRs) of hexameric DNA sequences (AGGNCA) separated by 1 bp (DR1). In the absence of ligands, PPAR-RXR heterodimers recruit corepressors and associated histone deacetylases and chromatin-modifying enzymes, silencing transcription by so-called active repression [22][23][24]. Ligandbinding induces a conformational change in PPAR-RXR complexes, releasing repressors in exchange for coactivators. Ligand-activated complexes recruit the basal transcriptional machinery, resulting in an enhanced gene expression.
PPARα is highly expressed in the liver, cardiac muscle, and digestive tract, and regulate the expression of target genes involved in lipid catabolism. Activators of PPARα, such as fibrates, decrease circulating lipid levels and are commonly used to treat hypertriglyceridemia and other dyslipidemic states [25]. PPARδ is expressed in many tissues including the skeletal muscle and brown adipose tissue. Recently, it has been suggested that PPARδ activation attenuates obesity and type-2 diabetes [26,27]. PPARγ is abundant in adipose tissues functioning as the key transcription factor for adipogenesis. Synthetic ligands for PPARγ, such as thiazolidinediones, are increasingly used to treat type-2 diabetes [28].
PPARs are involved in not only energy homeostasis but also inflammation. PPARα regulates inflammatory processes, mainly by inhibiting inflammatory gene expression. In recent years, several molecular mechanisms responsible for the immunosuppressive effects of PPARα have been uncovered [29]. These include interference with several proinflammatory transcription factors [30]. The number of studies that have addressed the role of PPARδ during inflammation is limited. So far, an anti-inflammatory effect has been observed in macrophages suggesting a possible role for PPARδ in the process of atherogenic inflammation. [31]. Similar to PPARα, PPARγ is involved in governing inflammatory response, particularly in macrophages. Currently, two different molecular mechanisms have been proposed by which anti-inflammatory actions of PPARγ are effectuated: (1) via interference with proinflammatory transcription factors [32] and (2) by preventing removal of correpressor complexes from gene promoter regions resulting in suppression of inflammatory gene transcription [33].
Recently, it has been indicated that obesity is associated with a low-grade chronic inflammation state [34]. The inflammatory condition in obesity is increasingly being recognized as an important contributor to the development of metabolic syndrome and its associated complications. Adipocytes can secret cytokines involved in inflammation, such as adiponectin, monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factor-α (TNF-α) [35]. MCP-1, a member of the CC chemokine superfamily, plays a pivotal role in monocyte/macrophage trafficking and activation [36]. Macrophages also produce various proinflammatory factors including MCP-1 and TNF-α. Macrophage-derived TNF-α establishes a vicious cycle that augments inflammatory changes and insulin resistance in obese adipose tissues [37]. Therefore, to prevent obesity-related inflammation, it is important to decrease the production of obese-adiposetissue-derived proinflammatory factors such as MCP-1 and TNF-α.
Several herbal and dietary plants improve medical conditions including diabetes mellitus, hyperlipidemia, and cardiovascular disease associated with an abnormality of lipid metabolism [38,39]. To screen for novel natural ligands for PPARs, we have evaluated PPAR ligand activities for various terpenoids in an advanced highly sensitive system with the coexpression of a coactivator for nuclear receptors, the cAMP-response element-binding protein (CREB)-binding protein (CBP), developed by modifying the luciferase reporter assay system [40]. Hereinafter, we describe several terpenoids, identified as novel PPAR ligands, in our PPAR ligand screening.

Novel Functions of Dietary Terpenoids as PPAR Ligands
3.1. Isoprenols. We carried out screening for novel PPAR ligands in natural compounds contained in medicinal plants.
We used several terpenoids including carotenoids and polyisoprenoid alcohols (isoprenols) for the screening, because these compounds are contained in many medicinal and dietary plants [41]. These terpenoids have multifunctions such as the suppression of tumor proliferation [41][42][43], apoptosis-inducing activity [9], and cation channel regulation [44]. Some terpenoids, which are intermediates in cholesterol synthesis, regulate the activity of 3-hydroxy-3methylglutaryl-coemzyme A (HMG-CoA) reductase, a key enzyme in cholesterol synthesis by controlling the degradation of the enzyme [45,46]. Such functions of dietary terpenoids are significant for the trials to manage disease conditions such as cancers or cardiovascular diseases using food factors. As shown in Figure 3, several terpenoids activated PPARγ at a concentration of 50 or 100 μM [40]. In this assay, we identified that isoprenols, such as geraniol, farnesol, and geranylgeraniol (chemical structures are shown in Figure 2) have a potential to activate PPARγ as novel ligands. At  Figure 3: Effects of various terpenoids on the activation of PPARγ ligand and chemical structures of active terpenoids. PPARγ activity in monkey CV-1 kidney cells was determined by luciferase reporter assay using advanced highly sensitive GAL4/PPARγ chimera system described in [40]. After 24 hours of incubation with or without each terpenoid at 50 or 100 μM, luciferase activity was measured. The activity of a vehicle control was set at 100% and the relative luciferase activities are presented as fold induction compared with that of the vehicle control. The values are means ± S.E.M. of 3-4 replicates. B: Chemical structures of active terpenoids.
100 μM, the activations by geraniol, farnesol, and geranylgeraniol were 2.2-, 4.1-, and 3.7-fold that by the vehicle control, respectively. On the other hand, squalene had no effect on PPARγ transactivation. Although geraniol had no effect on PPARα, farnesol and geranylgeraniol also dosedependently activated PPARα (10-and 8.6-fold increases at a concentration of 100 μM compared with vehicle controls, respectively) in the PPARα ligand assay system [40]. These activities were so potent and nearly the same as that of 10 μM fenofibrates, one of the fibrates (antihyperlipidemia drugs) used as a positive control for PPARα. In this regard, these farnesol and geranylgeraniol isoprenols have the effects of dual activation of PPARγ and PPARα. PPARγ activation in adipose tissues results in the improvement of insulin resistance [46] and PPARα activation in the liver induces the lowering of circulating lipid levels [47]. These effects are due to the regulation at mRNA expression levels of target genes of PPARs. The addition of each isoprenol induced mRNA expression of PPAR target genes in 3T3-L1 adipocytes and HepG2 hepatocytes [40]. Therefore, it is possible that isoprenols could regulate insulin resistance and/or circulating lipid levels. The finding of the dual activation of PPARγ and PPARα by isoprenols is very important for thinking of the mechanisms understanding the effects of medicinal plants and valuable for the management of diabetic and hyperlipidemic conditions in herbal medicine. Indeed, in our preliminary study, farnesol ameliorated hyperglycemia in high fat diet-fed wild-type mice but not in PPARα deficient mice (Goto et al., unpublished data). These findings indicate that improvement of obesity-associated metabolic disorders by farnesol is mainly dependent on PPARα activation. These results provide not only a significant molecular basis on how herbal plants containing phytochemicals such as isoprenols induce the improvement of diabetes or hyperlipidemia, but also possibilities that phytochemicals might have therapeutic applications in lipid abnormalities, such as obesity, diabetes mellitus, and hyperlipidemia.

Phytol.
Phytol, a diterpene alcohol, which is a carbon side chain of a chlorophyll molecule (Figure 2), is involved in the production of energy from light. Phytol is a plastidial terpenoid and synthesized via the MEP pathway in plastids [48]. Since almost all photosynthetic organisms use chlorophylls, phytol is also abundantly present in nature including various vegetables. It is suggested that chlorophyll molecules are partially digested and the phytol moiety is released in animals [49]. Then, the released phytol is absorbed in the small intestine and converted to phytanic acid in the liver.
Phytanic acid is a branched-chain, terpenoid-derived fatty acid constituent of diet ( Figure 2). In surveys of phytanic acid content of a variety of food products, high levels were indeed found in products such as milk, butter, cheese, meat from cows, sheep, and some fish and fish oils, whereas no phytanic acid is present in vegetables [50]. This compound can also be produced from the conversion of dietary phytol in the body [51]. Phytanic acid has been reported to activate PPARγ and the retinoid-X-receptor (RXR) [52,53] so that differentiation is stimulated in both white and brown adipocytes [52]. In addition, phytanic acid stimulates PPARα to regulate lipid metabolism in some types of cell [54]. Therefore, the intake of phytol as a precursor of phytanic acid may be valuable for the management of lipid metabolism through the activation of PPARs. Indeed, a phytol-enriched diet increased plasma and hepatic levels of phytanic acid, and induced the mRNA expression of PPARα target genes involved in peroxisomal and mitochondrial βoxidation and fatty acid metabolism [55].
However, since the conversion of phytol into phytanic acid is not very rapid, a phytol-enriched diet also induced accumulation of phytol in the liver [56]. Moreover, as described previously, several terpenoids, which resemble phytol in structures, activate PPARs in adipocytes and hepatocytes [40]. In this sense, it must be valuable to analyze the effects of phytol itself as an activator of PPARs.
Therefore, we evaluated the effects of phytol on PPAR activity using our advanced highly sensitive luciferase assays (Figure 3). Among the PPAR isoforms, PPARα was activated the most markedly following the addition of phytol [57]. The effects of phytol on PPARα activation were larger than those of phytanic acid under our experimental conditions. Phytol induced the mRNA and protein expression of PPAR target genes in a manner dependent on the level of PPARα expression in HepG2 hepatocytes. In our in vitro coactivator recruiting assay, it was revealed that phytol can activate PPARα directly [57]. These findings indicate that phytol itself can directly bind to PPARα as its ligand.
Because the activation of PPARα is one of the most important factors in lipid metabolism in peripheral tissues including the liver and muscles, the ability of phytanic acid and phytol to activate PPARα is very important in the management of lipid metabolism using food factors. Induction of PPARα target gene expression in mice fed a phytol-enriched diet [55] is likely due to not only phytanic acid but also phytol. It is considered that such effects of phytol are valuable for the control of lipid abnormalities in common diseases including obesity, diabetes, and hyperlipidemia through PPARα activation in the liver.

Abietic Acid
Derivatives. The amount of variety of hydrocarbons and their derivatives used in industrial and commercial activities has been increasing over the years. Abietic acid is a tricyclic-diterpene carboxylic acid (Figure 2), and is the main component of the rosin fraction of oleoresin synthesized by conifer species, such as grand fir (Abies grandis) and lodgepole pine (Pinus contorta) [58]. Abietic acid is commonly used as a fluxing agent in solder, as a paper sizing agent to make paper more water resistant, and in printing inks, adhesives, and plasticizers [59]. Moreover, it has been reported that abietic acid is a bioactive compound and it has an anti-inflammatory effect. In lipopolysaccharide (LPS)stimulated macrophages, abietic acid suppresses production of prostaglandin E2 (PGE2) in vitro and in vivo [60].
To investigate whether the activation of PPARs is related to the anti-inflammatory effects of abietic acid and its derivatives, we evaluated the effects of abietic acid and its derivatives on PPAR activity (Figure 2). Abietic acid and dehydroabietic acid, one of major components of colophony (also known as Rosin and pine resin), potently activated both PPARα and PPARγ but not PPARδ [61,62]. Similarly to thiazolidinedione, a synthetic PPARγ ligand, abietic acid suppressed mRNA expressions of TNF-α and cyclooxygenase 2 (COX2), which are induced in inflammatory reactions, in LPS-stimulated macrophages [61]. Dehydroabietic acid stimulated PPARα and PPARγ more potently than abietic acid [62]. Dehydroabietic acid significantly suppressed the production of proinflammatory mediators such as MCP-1, TNF-α, and NO in LPS-stimulated macrophages and in the coculture of macrophages and adipocytes [62]. In obese diabetic KK-Ay mice, dietary dehydroabietic acid suppressed obesity-associated elevation of circular MCP-1 and TNF-α levels and their mRNA expressions in white adipose tissues. Moreover, dehydroabietic acid improved carbohydrate and lipid metabolism [63]. These findings indicate that the anti-inflammatory effects of abietic acid and dehydroabietic acid are at least partly due to the activation of PPARs. Additionally, it is suggested that these compounds can be used not only for anti-inflammation but also for regulating carbohydrate and lipid metabolism and atherosclerosis.

Auraptene.
Citrus-fruit-derived compounds have many beneficial bioactivities (e.g., anticarcinogenic, antihypertension, and anticardiovascular disease effects) [64,65]. Through our screening for PPARs ligands (Figure 2), we identified auraptene, a geraniol coumarin ether, as a novel PPARα and PPARγ ligand [66,67]. Auraptene (Figure 2) occurs in a variety of citrus fruits. It has been reported that auraptene has anti-inflammatory and anticarcinogenic activities. In cultured adipocytes, auraptene upregulated an antiatherosclerotic, antidiabetic, and anti-inflammatory cytokine, adiponectin, and downregulated a proinflammatory cytokine, MCP-1. These effects disappeared in the presence of GW9662, a PPARγ antagonist [66], suggesting that auraptene improves adipocytokine profiles via the activation of PPARγ. In addition, mRNA expressions of several PPARα target genes involved in FA catabolism, were also induced in PPARα-expressing HepG2 hepatocytes by auraptene treatment [67]. It is likely that auraptene regulates the mRNA expressions of both PPARγ and PPARα target genes as a dual agonist, and these activities might contribute to the anti-cardiovascular disease effect of citrus fruits.

Bixins.
Annatto obtained from the pericarp of seeds from Bixa orellana is a natural pigment extensively used in many processed foods [68,69]. Bixin and norbixin (Figure 2), which are carotenoids, are the main components of this pigment, and have been reported to possess antioxidative and anticarcinogenic effects [70][71][72]. Furthermore, it has also been indicated that both annatto extract and norbixin have hypoglycemic effects in nonobese dogs and mice, respectively [73,74]. In PPARγ ligand assay, both bixin and norbixin activated PPARγ (Figure 2), and bixin induced PPARγ target genes in 3T3-L1 adipocytes, resulting in the promotion of adipocyte differentiation and insulinstimulated glucose uptake [75]. Therefore, the hypoglycemic effects of annatto and norbixin might be caused by the activation of PPARγ.

Conclusions
In this paper, we mentioned the diversity of terpenoids, functions of PPARs, and several terpenoids activating PPARs. The prevalence of obesity worldwide has progressively increased over the past decades. This unabated rise has spawned proportionate increases in obesity-associated metabolic disorders. Currently, synthetic PPAR agonists are widely used for the treatment of metabolic disorders. Daily intake of dietary terpenoids, which activate PPARs as we described above, may be valuable for the control of carbohydrate and lipid disorders. Dietary patterns rich in vegetables and fruit are associated with a lower prevalence of metabolic syndrome [65,76]. Because most of the terpenoids are of plant origin and they are contained in vegetables and fruit, dietary terpenoids may contribute to a decrease in risk of metabolic syndrome. Moreover, because the terpenoids constitute one of the largest families of natural products, more potent and useful PPAR activators may exist.