Psyche

Volume 2012 (2012), Article ID 459683, 8 pages

doi:10.1155/2012/459683

Research Article

Interaction between Bees and the Tristylous Flowers of Oxalis cytisoides Mart. & Zucc. (Oxalidaceae)

Cristiane Krug,¹ Cláudia Inês Silva,² and Isabel Alves-dos-Santos³

¹Centro de Pesquisa Agroflorestal da Amazônia Ocidental, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Rodovia AM-10 Km 29, Zona Rural, P.O. Box 319, 69010-970 Manaus, AM, Brazil

²Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP/USP), Avendia Bandeirantes 3900, 14040-901 Ribeirão Preto, SP, Brazil

³Departamento de Ecologia, Instituto de Biociências da Universidade de São Paulo (IBUSP) and Cidade Universitária, 05508-900 São Paulo, SP, Brazil

Received 3 August 2012; Revised 5 October 2012; Accepted 8 October 2012

Academic Editor: Kleber Del-Claro

Copyright © 2012 Cristiane Krug et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

The interaction of bees with the tristylous flowers of Oxalis cytisoides Mart. & Zucc. (Oxalidaceae) was evaluated. The study was conducted in a semideciduous forest at the Fritz Plaumann State Park in Concórdia, Santa Catarina state. Two Oxalis cytisoides aggregations were found and the flower visiting bees were observed. The 3 floral morphs were found at the following proportions: 16 long-, 37 mid-, and 34 shortstyled individuals (). Anthesis lasted one day (6:30 AM to 3 PM). No fruit was formed in the autogamy test; thus, pollination was dependent on the visitors. The pollen grain size varied between the stamens and morphs and formed subsets in accordance with the stigma height (long/mid/short). We collected 165 bees from 30 species visiting the flowers. Hypanthium divaricatum was the most abundant bee species (34%) and the males were often observed patrolling the flowers in search of females for mating. Analysis of the pollen loads from 34 females showed that 27 carried O. cytisoides pollen. The most frequent bees that carried O. cytisoides pollen grains on their bodies were considered pollinator agents, responsible for transferring pollen grains among the floral morphs.

1. Introduction

Heterostyly is a rare phenomenon among plants that has been observed in 28 angiosperm families [1]. Heterostylous species have flowers with different morphs in their populations and may be distylous or tristylous. Each plant holds just one type of flowers. Tristyly is a more complex and rare type of heterostyly, which has been reported in seven botanical families, including Oxalidaceae [1–5].

Charles Darwin formulated an explanation for the adaptive function of heterostyly in 1877, which suggested that the anthers and stigma were positioned to promote cross-pollination between the floral morphs. Darwin [6] also reported observations from Fritz Müller in Santa Catarina for the genus Oxalis, who found that flowers do not produce seeds at sites with only one floral morph. However, when the three morphs were planted in a garden, many seeds were produced.

The tristyly species comprises three floral morphs that differ in stamen filament height, pistil style position, pollen grain size and self-compatibility systems. Cross-pollination mediated by insect visitors is favored for this type of flowers [2, 6–9]. The three floral morphs in tristyly populations likely represent the maximum number of sexual polymorphisms in plants that promote cross-pollination through pollinator contact geometry [10]. For legitimate cross-pollination plant requires vectors able to transfer pollen from flowers with long, mid, or short-level anthers to flowers with long-, mid-, or short styles, respectively [7, 9, 11]. All other filament-style length combinations result in little or no seed set [12].

The family Oxalidaceae has radial bisexual flowers with five free sepals and five distinct petals that are slightly connate and often convoluted [13]. There are typically ten stamens with connate filaments at the base; the external filaments are shorter than the internal filaments; and nectar is produced at the base of them or in glands that may alternate with the petals. Typically, the flowers have five styles and stigma that are often globular or punctate [14]. Three Oxalidaceae genera have been observed in Brazil; one of them, Averrhoa, (which includes starfruit) is native to Asia, but it is often cultivated in Brazil [13]. Oxalis is the largest genus in the family, with approximately 800 species; it is cosmopolitan, and Africa and the Americas are the centers for its diversity. There are approximately 114 Oxalis species in Brazil [14]. According to Lourteig [13], Oxalis cytisoides Mart. & Zucc. is widely distributed from the northeastern (Ceará) to south regions (Rio Grande do Sul) in Brazil and in Argentina. Oxalis cytisoides has an erect herbaceous or shrub habit that can grow up to 1 m high. This species is discontinuously distributed almost entirely across the state of Santa Catarina (southern Brazil), and it is characterized as a heliophile or with a preference for diffuse light and selective hygrophytes that often develop in altered areas, including tropical rain forests, mixed ombrophilous forests on the plateau, and the Upper Uruguay River forest. O. cytisoides flowers primarily in spring, which lasts until summer [13].

The frequency of the morphs in the populations and compatible system of many American Oxalis species were investigated by Mulcahy [15], Ornduff [2], Weller [16, 17], and Weller et al. [18], demonstrating that in some populations morphs are not in equilibrium and also showing the process of losing styled forms. Recently, Turketti [5] presented an extensive work on the expression of the tristyly in the genus Oxalis of South Africa, where it was found that most populations (of 58 different species) were at isopletic equilibrium, which means equal representation of style length morphs. The same thesis [5] studied a special case of two species of Oxalis section sagittate which have a different arrange and orientation of the anthers and stigma compared to other Oxalis. In these species self-compatibility was more expressive, but the dependence on the pollinator still needs to be tested.

The objective of this work was to study the interactions between the visiting bees and the tristyly flowers of Oxalis cytisoides, evaluating the pollination in natural conditions. Our questions were as follows: are there seeds that have been produced in natural conditions? If yes, which flower visitors are potentially responsible for the legitimate transference among the morphs? Additionally, the pollen grains of the different morphs were described.

2. Material and Methods

The study was conducted in the Concórdia municipality between September 2008 and April 2009 at the Fritz Plaumann State Park, 27°16′18′′S, 27°18′57′′S, 52°04′15′′W and 52°10′20′′W, which is predominantly composed of a semideciduous forest. In the study area, we observed two Oxalis cytisoides aggregations with clustered distribution of individuals. These aggregations were separated by approximately 1000 m, with sparsely individuals occurring along them. The plants in these aggregations were evaluated together. The three floral morphs of O. cytisoides were observed. The morphs did not exhibit remarkable structural differences except for the relative stamen positions, which had different filament and style heights, as schematized in Figure 1.

Figure 1: Scheme of Oxalis cytisoides Zucc flower morphology. L: long-styled flower with medium and short filaments. M: mid-styled flower with short and long filaments. S: short-styled flower with medium and long filaments. The arrows indicate the legitimate cross-pollination, according to the system of the heretostyly plants.

A self-fertilization treatment was used to evaluate Oxalis cytisoides self-pollination. In this treatment, 191 preanthesis flower buds from the three floral types were protected with bags made from voile fabric. This sample included 76 long-styled (L) flowers, 63 mid-styled (M), and 52 short-styled (S) flowers. The open flowers and fruits were removed from the branches when the flower buds were bagged. These buds were followed for 1-2 months to assess fruit formation. Pollination in a natural condition was analyzed through examining the fruit formation with seeds. At least 20 fruits of each floral morph were evaluated from each aggregation.

During the flowering period for O. cytisoides (September to April), we collected bees visiting the flowers once a month for two consecutive days for a total of 96 hours of sampling; the three flowers morphs were observed equally in time. The collected bees were killed and identified. In addition to collection, we observed the behavior of the bees at the flowers from the three morphs for 30 hours to assess the contribution of bees to O. cytisoides pollination. The bee specimens were deposited in the Entomological Collection Paulo Nogueira Neto (CEPANN) of the Bee Laboratory at the Institute of Biosciences in the University of São Paulo.

There are morphological differences in pollen grains from anthers of different filament sizes in Oxalis flowers [19]. Based on this information, the O. cytisoides pollen grains were analyzed separately to investigate the pollen morphology in the three morphs, samples with stamen anthers that had medium (m) and long filaments (l) in S flowers, short (s) and long (l) filaments in M flowers, and short (s) and mid (m) filaments in L flowers. Pollen grains were removed from the anthers of the three stamen types with different size filaments; they were described separately, and the grain size, and shape were considered. The pollen grains were acetolyzed following the method proposed by Erdtman [20] and subsequently mounted on slides following Barth [21]. For each sample, twenty pollen grains were measured along the polar and equatorial axes in accordance with Silva et al. [22]. A single factor analysis of variance (ANOVA) was used to evaluate the difference in size between the pollen grains [23]. The size of pollen grains is given by the measure from the longest axis in the equatorial view. The data on the pollen grain size were analyzed using the median from boxplots, which were plotted using the program R [24], graphics package version 2.13.0.

Samples of the pollen load from the scopae of the visiting female bees on the O. cytisoides flowers were collected for analysis. The pollen grains were subjected to the acetolysis process described above, and they were qualitatively analyzed for the presence or absence of O. cytisoides pollen.

This work was performed with the authorization (number 13486-2) for collection and transportation of biological material by IBAMA/SISBIO (Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis/Sistema de Autorização e Informação em Bioversidade).

3. Results

A total of 87 flowering individuals were observed: 42 at the first plant aggregation (11 long-, 13 mid-, and 18 short-styled morphs) and 45 at the second aggregation (5 long-, 24 mid-, and 16 short-styled morphs) (Figure 2). Anthesis began at dawn (6:30), and flower abscission began at approximately 15:00.

Figure 2: Oxalis cytisoides. (a) Plant habit. (b) Flower and buds. (c)–(e): Stamens and pistils from L (c), M (d), and S (e) morphs. Photo scales (c)–(e) = 5 mm.

In self-fertilization test with the three morphs, no fruit was formed in the 191 buds analyzed. In contrast, all those fruits sampled in natural conditions presented seeds.

The pollen grains from O. cytisoides (Figure 3) are monads, small and medium size, radial, isopolar, subtriangular in area, subprolate to prolate in shape (Table 1), and tricolpate and have colpate furrows, lolongate endoapertures, and reticulated exine.

Table 1: Means (±standard deviation) for pollen grain sizes from the three Oxalis cytisoides floral morphs at the equatorial and polar perspectives.

Morph
Filaments
Equatorial
Polar
P/E

M
s
24.52 (±1.00)
32.49 (±1.36)
1.32 (±0.06)

L
s
23.89 (±1.17)
33.30 (±1.98)
1.39 (±0.06)

S
m
27.59 (±1.42)
37.30 (±1.65)
1.35 (±0.06)

L
m
26.51 (±0.90)
37.90 (±1.10)
1.43 (±0.05)

S
l
28.89 (±1.13)
40.63 (±1.96)
1.41 (±0.09)

M
l
27.74 (±1.28)
40.59 (±2.67)
1.46 (±0.06)

S: short, M: medium, and L: long.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

Figure 3: Pollen grains from Oxalis cytisoides. ((a)–(c)) Polar perspective for pollen grains from long (a), mid (b), and short (c) anthers. ((d)–(i)) Equatorial perspective for pollen grains in long ((d) and (g)), mid ((e) and (h)) and short ((f) and (i)) anthers. Capital letters are the floral morph (style height) and lower letters are the anthers from the pollen grain origin, for example: S l = pollen came from the long filaments (l) of the short styled morph (S).

The pollen grain size varied between the stamens and morphs (Table 1). Significant differences were observed for the pollen grain sizes in polar view from the six samples (mean square, 3.4609; degrees of freedom, 114.00, ). We also observed that the pollen from short-, mid-, and long-stamen anthers was grouped (Figure 4), forming subsets in accordance with the stigma height.

Figure 4: Box plot of pollen grain sizes for the three flower morphs measured from the polar perspective. Box plots show the medians as well as 1° and 2° quartiles and ranges; the circles indicate extreme values (outliers).

3.1. Floral Visitors

A total of 165 individuals were sampled and distributed over 30 species and four bee families (Table 2). The visitors were more abundant in December and February, although the number of flowering plants was practically the same throughout the flowering months.

Table 2: Visiting bees of Oxalis cytisoides flowers in Concordia, Santa Catarina, southern Brazil.

Family
Species
Ind.
Month

Andrenidae
Anthrenoides meridionalis (Schrottky, 1906)*
2 (2 F)
Nov

Psaenythia bergii Holmberg, 1884*
2 (2 F)
Nov

Apidae
Ceratina (Calloceratina) sp. 2
1 (1 F)
Dec

Ceratina (Crewella) sp. 12
1 (1 F)
Sep

Ceratina (Crewella) sp. 16*
15 (12 F, 3 M)
Feb-Mar, Sep–Dec

Lophopedia nigrispinis (Vachal, 1909)*
23 (16 F, 7 M)
Feb, Sep, Nov-Dec

Odyneropsis sp.
1 (1 F)
Dec

Paratetrapedia (Paratetrapedia) sp. 1
5 (1 F, 4 M)
Feb

Paratetrapedia (Paratetrapedia) sp. 2*
7 (6 F, 1 M)
Nov–Mar

Paratetrapedia (Paratetrapedia) sp. 4
6 (6 M)
Feb, Nov

Tetrapedia diversipes Klug, 1810*
13 (13 F)
Feb-Mar, Nov-Dec

Trigona spinipes (Fabricius, 1793)
1 (1 F)
Sep

Halictidae
Augochlora (Augochlora) sp. 1†
1 (1 F)
Nov

Augochlora (Augochlora) sp. 3
1 (1 F)
Oct

Augochlora (Augochlora) sp. 4
1 (1 M)
Dec

Augochlora (Augochlora) sp. 6
1 (1 F)
Dec

Augochlora (Oxystoglossela) sp. 4
1 (1 F)
Feb

Augochlorella sp. 2
7 (7 F)
Feb, Nov-Dec

Augochlorella sp. 5
5 (4 F, 1 M)
Oct–Jan

Augochloropsis sp. 1
1 (1 F)
Jan

Augochloropsis sp. 2
1 (1 F)
Dec

Augochloropsis sp. 12
1 (1 F)
Feb

Neocorynura sp.
1 (1 F)
Dec

Megachilidae
Anthidulum mourei Urban, 1993
1 (1 M)
Nov

Hypanthidium divaricatum (Smith, 1854)*
57 (17 F, 40 M)
Nov–Apr

Hypanthidium obscurius Schrottky, 1908†
3 (1 F, 2 M)
Nov–Jan

Megachile (Leptorachina) sp. 1
2 (2 M)
Jan, Mar

Megachile (Austromegachile) susurrans Haliday, 1836
2 (2 F)
Dec

Moureanthidium paranaense Urban, 1995
1 (1 M)
Nov

Moureanthidium subarenarium (Schwarz, 1933)
1 (1 M)
Nov

Total

165
Sep–Apr

Ind.: number of individuals, month: collection month, *O. cytisoides pollen grains in the scopae pollen load, and †number of O. cytisoides pollen grains in the scopae. F: female and M: male.

In general the bees visited more than one flower on the same plant and different plants in the same aggregation with no observed preference for a particular morph. Lophopedia nigrispinis (Vachal, 1909) (Apidae), Tetrapedia diversipes Klug, 1810 and Ceratina sp. remained for a few seconds in the flowers and visited between 2 and 5 flowers, preferentially collecting nectar. Hypanthidium divaricatum (Smith, 1854) (Megachilidae) and Ceratina sp. (Apidae) actively collected pollen and nectar.

Among the sampled bees, the most abundant in the flowers were H. divaricatum (Figures 5(a) and 6(a)–6(c)) and L. nigrispinis (Figures 6(d)–6(f)), which corresponded to 34% and 14% individuals, respectively. Pollen grains were often observed adhered to the mouthparts of the sampled bees (Figures 6(c) and 6(f)). H. divaricatum males were frequently observed patrolling O. cytisoides flowers searching for females, and a mate was recorded on certain occasions (Figure 5(b)).

(a)

(b)

Figure 5: Hypanthidium divaricatum visiting (a) and mating (b) at Oxalis cytisoides flowers.

(a)

(b)

(c)

(d)

(e)

(f)

Figure 6: (a)–(c): Hypanthidium divaricatum ((a): male dorsal view, (b): female ventral view with loaded abdominal scopae (arrow) and (c): close-up of the male mouthparts with pollen grains attached to the hairs). (d)–(f): Male Lophopedia nigrispinis (d: dorsal view, e: ventral view and f: close-up of mouthparts with pollen grains attached to the hairs).

Oxalis cytisoides pollen was observed in 25 samples of pollen material removed from the scopae of 32 females that belonged to nine bee species, which indicates that these females effectively collect this floral resource (Table 2).

4. Discussion

The three floral morphs of Oxalis cytisoides were clearly distinguished in the studied area. The self-fertilization (bagged flowers) tests showed no fruit production, even in the mid- and short-styled flowers, which would be easily contaminated with pollen from the above anthers. On the other hand flowers left under natural conditions produced fruits, which demonstrated participation by pollinators. In the studied area we sampled 30 bee species visiting the flowers of O. cytisoides. Among them there were some very frequent in the visits and that were flying 4–6 months during the whole flowering period of the plant species, like Hypanthidium divaricatum, Lophopedia nigrispinis, and Ceratina sp. 16. These three species are potential candidates for pollinators, due to the frequency and abundance in the flowering period, and also because the females collected actively pollen from Oxalis flowers (demonstrated by behavior and the analysis of the pollen load of the scopae). Pollen collection requires more time and ability at the flowers during the visits. This increases the permanence time at the flower and contact with the reproductive parts, assisting in pollen transference to the stigma. Simultaneously, during the visits, the pollen can adhere to the visitor bodies, like to the forehead, abdomen, and thorax. These pollens adhered to the hairs are likely more important for pollen transfer than the pollens in the scopae, because of the position they will touch into the next flower. Therefore, the bee species with these characteristics were most likely to promote cross-pollination for O. cytisoides in the studied area, guarantying the verified fruit set and also the maintenance of the three morphs in the populations. It is known that the trimorphic condition tends to break down the polymorphism and evolves a homostylous condition in populations with a deficit of effective pollinators [4, 12].

Hypanthidium divaricatum shows a special behavior and preference to the flowers of O. cytisoides. The males patrol and seek for females to copulate in the plants. This behavior indicates that males recognize this plant as a preferred species of their females, showing specialization. A similar fact was also reported for Ancyloscelis bees and plants of the Pontederiaceae family [25] and for Cephalurgus anomalus Moure and Oliveira and plants of the Malvaceae family [26, 27]. Further results reinforcing the specialization were attested by the pollen load of the females of H. divaricatum, which were carrying most grains of O. cytisoides. This bee species was found visiting other plants in Concórdia, but of 86 individuals collected in the region 46 (53%) were visiting O. cytisoides flowers [28].

According to our results H. divaricatum is flying between November and April covering the most period of flowering of O. cytisoides. Since the flower season for this species starts in September, probably the 2 other bee species (Ceratina sp. 16 and L. nigrispinis) with high frequency in the flowers may act as effective pollinators at the beginning of the season.

Luo et al. [29] studied the South American species Oxalis debilis Kunth, which was introduced in China and found that it does not predominantly reproduce vegetatively, as was previously assumed for this species. The pollination of O. debilis was performed by bees that collected nectar (Apis cerana Fabricius, 1793) as well as pollen (Ceratina (Pithitis) smaragdula Fabricius, 1787, and Ceratina sp.). According to Björkman [30], disc-shaped and small flowers allow for nectar access by several species, which facilitates the cross-fertilization of the plant. This is probably the case of O. cytisoides studied herein which received a spectrum of 30 bee species, including short and long tongued bees. But, it is important to emphasize that to the plant visitors with high frequency and carrying pollen grains are likely more effective for the cross-pollination than those sporadically visitors. However, future studies on stigmatic receptivity and specificity in O. cytisoides are necessary for a more accurate conclusion about the role of each visitor.

The morphology and ornamentation of the pollen grains from O. cytisoides are consistent with the description for nine species in the Oxalis genus by Rosenfeldt and Galati [19]. The largest pollen grains were produced in the high level anthers (long stamens), intermediate size pollen was produced by mid-level anthers, and smaller grains were produced by low level anthers, as has been reported for other Oxalis species [14, 19, 31] and would be expected in a tristyly species. At the load of the bees it was not possible to distinguish or quantify precisely the anther origin of the pollen grains, because of some overlap in their size. But the three types of pollen were presented in the samples of the seven bee species carrying Oxalis pollen (Table 2). We believe that female bees are able to collect the pollen grains from all the anther levels without a problem, since the flowers of Oxalis do not have special morphology to hide the resource, like it is known for the tristyly Eichhornia species [9, 12].

Compatibility tests between morphs of species of the genus Oxalis studied by Ornduff [2] and Pacheco and Coleman [31] revealed that legitimate pollination (following heterostyly scheme) is more successful than illegitimate pollination for frequency and seed production. Although we did not conduct cross-pollination tests, we found that under natural conditions fruits were produced, demonstrating the importance of the local visiting bees as agents for the correct pollen transference.

Finally, to the bee perspective, it is necessary to highlight that O. cytisoides seems to be an important source of floral rewards for the local bee fauna. Certain studies refer to Oxalis as a flower resource for honeybees [32, 33]. But at the study area we verified that it deals with a relevant source of nectar and pollen for many native bee species, especially solitary bees. According to Krug [28], about 27% of the known Apiformes species occurring in the Fritz Plaumann Park in Concordia were visiting the flowers of O. cytisoides.

Acknowledgments

The authors thank the (Conselho Nacional de Desenvolvimento Científico e Tecnológico) (CNPq) for the doctoral scholarship to the first author and the Graduate Program of Entomology (FFCLRP) University of São Paulo. They also thank the Management Team at the Fritz Plaumann State Park and the IBAMA for the collection license. A special thank you is extended to the family members of the first author who kindly helped in the field trips: Felipe Lenhard, Marcos A. Krug, Orlando A. Krug, and Noeli Krug.

References

S. C. H. Barrett, L. K. Jesson, and A. M. Baker, “The evolution and function of stylar polymorphisms in flowering plants,” Annals of Botany, vol. 85, pp. 253–265, 2000.

R. Ornduff, “The breeding system of Oxalis suksdorfii,” American Journal of Botany, vol. 51, no. 3, pp. 307–314, 1964.

R. Ornduff, “Heterostyly in south african flowering plants: a conspectus,” Journal of South African Botany, vol. 40, pp. 169–187, 1974.

S. C. H. Barrett, “The evolutionary biology of tristyly,” Oxford Surveys in Evolutionary Biology, vol. 9, pp. 283–326, 1993.

S. S. Turketti, A study of tristyly in south african Oxalis [Ph.D. thesis], University of Stellenbosch, Stellenbosch, South Africa, 2010.

C. Darwin, The Different Forms of Flowers on Plants of the Same Species, Murray press, London, UK, 1884.

D. Charlesworth, “The evolution and breakdown of tristyly,” Evolution, vol. 33, no. 1, pp. 486–498, 1979.

S. C. H. Barrett and D. E. Glover, “On the darwinian hypothesis of the adaptive significance of tristyly,” Evolution, vol. 39, no. 4, pp. 766–774, 1985.

S. C. H. Barrett, “The evolution and adaptive significance of heterostyly,” Trends in Ecology and Evolution, vol. 5, no. 5, pp. 144–148, 1990.

S. C. H. Barrett and J. S. Shore, “New insights on heterostyly: comparative biology, ecology and genetics,” in Self-Incompatibility in Flowering Plants: Evolution, Diversity and Mechanisms, V. E. Franklin-Tong, Ed., pp. 3–32, Springer, 2008.

F. R. Ganders, “The biology of heterostyly,” New Zealand Journal of Botany, vol. 17, no. 4, pp. 607–635, 1979.

I. Alves dos Santos, “Flower-visiting bees and the breakdown of the tristylous breeding system of Eichhornia azurea (Swartz) Kunth (Pontederiaceae),” Biological Journal of the Linnean Society, vol. 77, no. 4, pp. 499–507, 2002.

A. Lourteig, “Oxalidáceas,” in Flora Ilustrada Catarinense, R. Reitz, Ed., Herbário Barbosa Rodrigues, Itajaí, Brazil, 1983.

J. D. Denardi, Estrutura e ontogênese de órgãos reprodutivos de connarus suberosus planch. (Connaraceae) e Oxalis cytisoides Zucc. (Oxalidaceae) [Ph.D. thesis], Universidade Estadual Paulista, 2008.

D. L. Mulcahy, “The reproductive biology of Oxalis priceae,” American Journal of Botany, vol. 51, no. 10, pp. 1045–1050, 1664.

S. G. Weller, “Dispersal patterns and the evolution of distyly in Oxalis alpina,” Systematic Botany, vol. 3, no. 1, pp. 115–126, 1978.

S. G. Weller, “Evolutionary modifications of tristylous breeding systems,” in Evolution and Function of Heterostyly, S. C. H. Barrett, Ed., pp. 247–270, New York, NY, USA, 1992.

S. G. Weller, C. A. Domínguez, F. E. Molina-Freaner, J. Fornoni, and G. LeBuhn, “The evolution of distyly from tristyly in populations of Oxalis alpina (Oxalidaceae) in the sky islands of the sonoran desert,” American Journal of Botany, vol. 94, no. 6, pp. 972–985, 2007.

S. Rosenfeldt and B. G. Galati, “Pollen morphology of Oxalis species from buenos aires province (Argentina),” Biocell, vol. 31, no. 1, pp. 13–21, 2007.

G. Erdtman, “The acetolized method. A revised description,” Svensk Botanisk Tidskrift, vol. 54, pp. 561–564, 1960.

O. M. Barth, “Glossário Palinológico,” Memorial do Instituto Oswaldo Cruz, vol. 63, pp. 133–162, 1965.

C. I. Silva, P. L. O. Ballesteros, M. A. Palmero, S. G. Bauermann, A. C. P. Evaldt, and P. E. Oliveira, Catálogo polínico: Palinologia aplicada em estudos de conservação de abelhas do gênero Xylocopa, EDUFU, Uberlândia, MG, Brazil, 2010.

Statistica 7.0 Software, StatSoft, Tulsa, Okla, USA, 2005.

R Development Core Team R, A Language and Environment for Statistical Computing, R Foundation for Statistical Computing, Vienna, Austria, 2011.

I. Alves-dos-Santos, “Aspectos morfológicos e comportamentais dos machos de Ancyloscelis Latreille (Anthophoridae, Apoidea),” Revista Brasileira de Zoologia, vol. 16, no. 2, pp. 37–43, 1999.

M. C. Gaglianone, “Biologia floral de espécies simpátricas de malvaceae e suas abelhas visitantes,” Biociências, vol. 8, no. 1, pp. 13–31, 2000.

E. F. Morato and L. A. O. Campos, “Partição de recursos de Sida Linnaeus e Malvastrum coromandelianum (Linnaeus) Garcke (Malvaceae) entre Cephalurgus anomalus Moure & Oliveira (Hymenoptera, Andrenidae, Panurginae) e Melissoptila cnecomala (Moure) (Hymenoptera, Apidae, Eucerini),” Revista Brasileira de Zoologia, vol. 17, no. 3, pp. 705–727, 2000.

C. Krug, A comunidade de abelhas (Hymenoptera—Apoidea) de duas áreas de interesse biológico e histórico em Santa Catarina [Ph.D. thesis], Universidade de São Paulo, Sao Paulo, Brazil, 2010.

S. Luo, D. Zhang, and S. S. Renner, “Oxalis debilis in china: distribution of flower morphs, sterile pollen and polyploidy,” Annals of Botany, vol. 98, no. 2, pp. 459–464, 2006.

T. Björkman, “The effectiveness of heterostyly in preventing illegitimate pollination in dish-shaped flowers,” Sexual Plant Reproduction, vol. 8, no. 3, pp. 143–146, 1995.

R. P. B. Pacheco and J. R. Coleman, “Reproductive morfology, genetic control and imcompatibility relations in tristylous Oxalis physocalyx (Oxalidaceae),” Revista Brasileira de Genética, vol. 12, no. 2, pp. 347–359, 1989.

C. A. L. Carvalho, L. C. Marchini, and P. B. Ros, “Plantas visitadas por Apis mellifera L. no vale do rio paraguassú, município de castro alves, bahia,” Revista Brasileira de Botânica, vol. 22, no. 2, pp. 333–338, 1999.

L. C. Marchini, A. C. Moreti, E. W. Teixeira, E. C. A. Silva, R. R. Rodrigues , and V. C. Souza, “Plantas visitadas por abelhas africanizadas em duas localidades do estado de São Paulo,” Scientia Agrícola, vol. 58, no. 2, pp. 413–420, 2001.