Neural Differentiation of Human Adipose Tissue-Derived Stem Cells Involves Activation of the Wnt5a/JNK Signalling

Stem cells are a powerful resource for cell-based transplantation therapies, but understanding of stem cell differentiation at the molecular level is not clear yet. We hypothesized that the Wnt pathway controls stem cell maintenance and neural differentiation. We have characterized the transcriptional expression of Wnt during the neural differentiation of hADSCs. After neural induction, the expressions of Wnt2, Wnt4, and Wnt11 were decreased, but the expression of Wnt5a was increased compared with primary hADSCs in RT-PCR analysis. In addition, the expression levels of most Fzds and LRP5/6 ligand were decreased, but not Fzd3 and Fzd5. Furthermore, Dvl1 and RYK expression levels were downregulated in NI-hADSCs. There were no changes in the expression of ß-catenin and GSK3ß. Interestingly, Wnt5a expression was highly increased in NI-hADSCs by real time RT-PCR analysis and western blot. Wnt5a level was upregulated after neural differentiation and Wnt3, Dvl2, and Naked1 levels were downregulated. Finally, we found that the JNK expression was increased after neural induction and ERK level was decreased. Thus, this study shows for the first time how a single Wnt5a ligand can activate the neural differentiation pathway through the activation of Wnt5a/JNK pathway by binding Fzd3 and Fzd5 and directing Axin/GSK-3ß in hADSCs.


Background
Mesenchymal stem cells (MSCs) reside in the bone marrow, peripheral blood, and adipose tissue and have a therapeutic potential in diseases such as multiple sclerosis [1], diabetes [2], stroke [3,4], and neurodegenerative disease [5]. MSCs self-renew and differentiate into bone, fat tissue, cartilage, muscle, marrow stroma, tendon, and ligament, both in vivo and in vitro, under appropriate culture conditions [6,7]. Understanding differentiation is important for several reasons. Firstly, differentiation is based on the process of acquiring specific functions so that stem cell research is likely to focus on improving the ability to guide the differentiation and to control their survival and proliferation [8,9]. Secondly, understanding differentiation may provide a proper strategy for clinical treatment of stem cells in neurological disorders. For therapeutic applications of MSCs, how stem cells switch from proliferation to differentiation and consistently sustain their abilities is very important. Recently, Wnt signalling has been implicated in the control of MSC differentiation [10,11], including osteogenic [12,13], chondrogenic, adipogenic, and myogenic differentiation [14].
Wnts are a family of glycoproteins that can signal through distinct intracellular pathways and regulate many developmental events in vertebrates and mammals [13,15]. As many as 19 mammalian Wnt homologues are known and shared features of all Wnts include a signal sequence for secretion, several highly charged amino acid residues, and many glycosylation sites [16]. Wnt proteins signal through two different pathways by binding with Frizzled (Fzd) receptors, which are seven-pass transmembrane proteins and are found as first receptors to transduce a Wnt signal [17]. At the cytoplasmic side, Fzd may interact directly with the Dishevelled (Dvl) protein, a known mediator of Wnt signalling [16]. In canonical pathway, Wnt/Fzd complex signals through activation of Dvl to inhibit the degradation of -catenin by the glycogen synthase kinase-3 (GSK3 ). The accumulation of the stabilized -catenin in cytoplasm is increased and enters the nucleus where it induces transcription of target gene expression. Noncanonical pathway, which is independent 2 Stem Cells International  Gene  Sense  Antisense  Wnt2  TGGTGGTACATGAGAGCTACAGGTG  CCCCATTGTACTTCCTCCAGAGATA  Wnt4  GAGGAGACGTGCGAGAAACTCAA  ATCCTGACCACTGGAAGCCCTGT  Wnt5a  TTTTTCTCCTTCGCCCAGGTTGT  GGCTCATGGCGTTCACCAC  Wnt11  ACAACCTCAGCTACGGGCTCCT  CCCACCTTCTCATTCTTCATGC  Fzd2  CCATCCTATCTCAGCTACAAGTTTCT  GCAGCCCTCCTTCTTGGTG  Fzd3  TCCCCTCTGCCTGTATGTGGTAGT  GCTGCTCACTTTGCTGTGGA  Fzd4  CTCGGCTACAACGTGACCAAGAT  AATATGATGGGGCGCTCAGGGTA  Fzd5  TGCTACCAGCCGTCCTTCAGT  CCATGCCGAAGAAGTAGACCAG  Fzd6  TTTTGTCTTTGTGCAACTCTGTTCA  AGCCAATTCTGGTCGAGCTTTTG  LRP5  TCATTGTGGACTCGGACATTTACTGG  GTAGAAAGGCTCGCTTGGGGACAG  LRP6  CATTGTCCCAGAGGCTTTCCTTTTG  TAGGTTTTCCACCCCATTCAGTCCA  Dvl1  CCGCTGACGGTGAAGAGTGACAT  CCTGACTGCGTGGGCTGCTG  RYK  ATTTCCTGCACTTCACCTGG  CTTTGGCCTCCAAAAGAGTG  GSK3  CAGCAGCCTTCAGCTTTTGG  CCGGAACATTGTCCAGCACCAG  -catenin  GCTGATTTGATGGAGTTGGACATGG  GCCAAACGCTGGACATTAGTGG  GAPDH  CATGACCACAGTCCATGCCATCACT  TGAGGTCCACCACCTGTTGCTGTA of -catenin, mediates several cellular processes through monomeric GTPases of the Rho family, mitogen-activated protein kinase like Jun N-terminal kinase (JNK), and changes in intracellular calcium levels [10,11,[16][17][18][19][20]. The Wnt signalling pathway plays an important role in the development of the central nervous system and regulates the formation and function of neuronal circuits by controlling neuronal differentiation [21]. Several groups reported that Wnt3a and Wnt5a increased neural differentiation of human or mouse neuronal progenitor cells [15] and Wnt3a promoted the neuronal differentiation of rat MSCs by upregulation of the -catenin expression [11]. Wnts also stimulated neural differentiation from embryonic, somatic, and neural stem cells [22,23]. However, the Wnt pathway in neural differentiation of human adipose tissue-derived stem cells (hADSCs) is not studied yet. In the present study, we established neural induced-hADSCs (NI-hADSCs) following our previous study [9] and figured out the Wnt signalling and related pathways in NI-hADSCs compared with primary hADSCs.

Preparation of hADSCs and Neural Induction of hADSCs.
In this study, we used human ADSCs, which were already isolated and characterized in our previous study [9]. In brief, fat tissue from the human earlobe was obtained from ten healthy donors 4-20 years of age according to the Guidelines of the Ethics Committee at Chonnam National University Medical School (IRB:I-2009-03-016). Isolated hADSCs were maintained in Dulbecco's modified Eagle's medium (DMEM, Hyclone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone) and 1% penicillin-streptomycin (Hyclone) at 37 ∘ C humidified incubator with 5% CO 2 . The hADSCs used in the present study were from passages 2 to 5. To induce neural differentiation, hADSCs were grown in DMEM containing 1% FBS and supplementary 100 ng/mL basic fibroblast growth factor (bFGF, Invitrogen Co., Carlsbad, CA, USA) for seven days and were then incubated in 10 M forskolin (Sigma Chemical Co., St. Louis, MO, USA) for the next seven days. After that, the cells were subjected to RT-PCR analyses, real time RT-PCR assay, and western blot study.

Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR).
To analyse the relative expression of different mRNAs, the amount of cDNA was normalized based on signals from the ubiquitously expressed GAPDH [9,24]. Total RNA was extracted from cultured cells by using TRI Reagent (Molecular Research Center Inc., Cincinnati, OH, USA) and reverse-transcribed to cDNA using Reverse Transcription System (Takara Bio Inc., Shiga, Japan). PCR primer (Bioneer Co., Daejeon, Korea) pairs for human were selected to discriminate between cDNA and genomic DNA by using individual primers specific to different exons, when possible. Amplification was carried out at 94 ∘ C for 2 min, followed by 35 cycles at 94 ∘ C for 1 min, at appropriate annealing temperatures for each primer for 1 min, and at 72 ∘ C for 1 min [9]. Forward and reverse PCR oligonucleotide primers selected to amplify the cDNA are listed in Table 1 and RT-PCR products were separated electrophoretically on 2% agarose gels (Sigma Chemical Co.).

Real Time RT-PCR.
For real time RT-PCR analyses, cDNA was synthesized using total RNA which was previously prepared from primary hADSCs and NI-hADSCs using TRIzol protocol. Real time RT-PCR was performed with SYBR Green Premix Ex Taq (Takara Bio Inc.) and Thermal Cycler Dice Real Time System (Takara Bio Inc.). Each sample was analysed in four replicate reactions of 20 L. (c and f) The expression of Dvl1 and RYK is decreased in NI-hADSCs. Also, there is no change in the expression of either GSK3 protein or -catenin. GAPDH was used as a control. The RT-PCR assay was repeated five times independently of different cells and the representative data are shown. The intensity of each gene was normalized to GAPDH. * < 0.05, * * < 0.01 compared with the primary hADSCs. C, primary hADSCs; NI, NI-hADSCs.

Statistics.
Protein and mRNA levels were quantified by measuring the optical density of each band using computerassisted densitometry (NIH Image analysis program, version 1.61). All values were expressed as the mean ± SEM. The oneway ANOVA test (Bonferroni post hoc comparison) was used to analyse differences between groups, with < 0.05 being considered significant.

Analysis of Wnt Signal-Related Genes in NI-hADSCs.
To identify Wnt pathway genes at the molecular level, we performed RT-PCR analysis of Wnt pathway components including 4 Wnt families, 5 Wnt receptors, and 2 Wnt coreceptors. Following our previous study [9], hADSCs, which were already isolated from human fat tissue and characterized as MSCs, were differentiated into NI-hADSCs. Wnt2, Wnt4, and Wnt11 gene expressions were decreased whereas the expression of only Wnt5a gene was increased in NI-hADSCs (Figure 1(a)). Wnt receptors (Fzd2, Fzd4, and Fzd6) and coreceptors (LRP5 and LRP6) were all downregulated in NI-hADSCs, except Fzd3 and Fzd5, which were not expressed in hADSCs (Figure 1(b)). In addition, the expressions of RYK, which is a Wnt ligand receptor that can bind to the Wnt ligand, and Dvl1 were decreased in NI-hADSCs. However, GSK3 and -catenin, which are the target genes for Wnt signalling, levels were not changed after neural differentiation (Figure 1(c)).
To confirm the expression of Wnt-related genes, we tested and analyzed Wnt genes and receptor related genes by real time RT-PCR. Interestingly, most of Wnt-related gene expressions were decreased but the expression of Wnt5a was highly increased after neural differentiation in hADSCs, significantly ( Figure 2).

Wnt Pathway in NI-hADSCs.
We examined whether Wnts could be involved in promoting the neural differentiation. For analysis of Wnt signal pathway, we studied the Wntrelated pathway using western blot analysis. Figure 3 showed that Wnt3 expression was decreased in NI-hADSCs compared with primary hADSCs. Importantly, Wnt5a/b protein was highly expressed in NI-hADSCs and those are consistent with RT-PCR results. So, we focused on the Wnt5a, one of the noncanonical Wnt molecules which are known to be expressed in proliferating cells and to increase during differentiation [15,25]. Noncanonical pathway does not necessarily bind LRP5/6 complex, so we did not study LRP expression. Dvl2 and Naked1 protein levels were downregulated after neural differentiation (Figures 3(a) and 3(b)). The expression of Axin 1 was not changed in both primary hADSCs and NI-hADSCs.

Signalling Pathway in NI-hADSCs.
Noncanonical Wnt signalling has been shown in two different ways such as Wnt4 Primary hADSCs NI-hADSCs * * * * * * * * * * * * * * * * * * * * * * * * * * P < 0.05 * * P < 0.01 To investigate the signalling pathways by which Wnt5a influences neural differentiation, we employed phosphorspecific antibodies to identify the signalling effectors activated by Wnt5a in NI-hADSCs. Western blotting was carried out to show expression of JNK and phosphor-JNK, key molecules of the noncanonical Wnt signalling pathway. The phosphor-JNK expression level was upregulated in relation to control GAPDH in NI-hADSCs compared with primary hADSCs (Figure 3(c)). Furthermore, extracellular signal-related kinase (ERK1/2) protein level was observed in primary hADSCs and downregulated in NI-hADSCs and phosphor-EKR1/2 level was not changed after neural differentiation. However, the GSK3 and phosphor-GSK3 protein levels were not changed in both the primary hAD-SCs and NI-hADSCs. The quantification data showed that the phosphor-JNK, phosphor-ERK1/2, and phosphor-PKC levels were increased in relation to total-JNK, total-ERK1/2, and total-PKC in NI-hADSCs, respectively (Figures 3(d)-3(g)). These data indicate that the JNK expression is the important modulator in hADSC during neural differentiation (Figure 4).

Discussion
In the last few years there have been wide interests in the role of Wnt signalling and it is also thought to play a key role in controlling stem cell fate. Thus, understanding the mechanisms that regulate Wnt signalling is of critical importance, especially in stem cell differentiation. In particular, Stem Cells International  (a and b). The expression of Wnt3 is decreased in NI-hADSCs, but interestingly Wnt5a/b expression is upregulated after neural differentiation compared with primary hADSCs. Dvl2 protein is very slightly changed between primary hADSCs and NI-hADSCs. However, the expression of Naked1 is downregulated in NI-hADSCs compared with primary hADSCs. (c-g) Western blot analysis for Wnt signal-related proteins. The expression of phosphor-JNK, which is one of the downstream noncanonical pathways, is upregulated in NI-hADSCs compared with primary hADSCs. Also, ERK1/2 protein level is decreased after neural differentiation. (d-g) Quantified results of protein expression. The western blot was repeated three times independently of different cells and the representative data are shown. The intensity of each gene was normalized to GAPDH or -catenin. * < 0.05 compared with the primary hADSCs.
Wnt5a/receptor tyrosine kinase-like orphan receptor 2 pathway regulated interleukin-1 or anti-tumor necrosis factorinduced differentiation of human MSCs into osteoblasts [12,13]. In addition, Wnt5a is known to be a mediator of axonal branching and growth in developing sympathetic neurons [26] and a promoter with JNK signalling of postsynaptic density in hippocampal neurons [17]. Here we aimed to study the expression of Wnt5a, which is a one of noncanonical  Previously, Wnt/Ca 2+ pathway was already studied for other groups [27,28]. Here, we demonstrate that Wnt5a upregulates ERK and phosphor-JNK signalling and activates the neural differentiation of hADSCs.
Wnts, in neural differentiation of stem cells and to investigate further the role of Wnt signalling and JNK pathway. We found that the Wnt5a gene was mainly expressed in NI-hADSCs and upregulated the Fzd3 and Fzd5. Inestrosa's group reported that normally Wnt5a was expressed early in development and stimulated dendrite spine morphogenesis in the hippocampus, where it played a trophic role in neuronal differentiation and modulation of synaptic activity [29].
We observed that Wnt5a upregulated the Fzd3 and Fzd5 expressions and promoted the neural differentiation of hADSCs (Figure 1), showing constant high levels followed by real time RT-PCR ( Figure 2). This expression pattern suggests that this ligand may have a role during neural differentiation. Yu et al. reported that Wnt3a and Wnt5a increased neuronal differentiation of neural progenitor cells [15]. Interestingly, our results showed that only Wnt5a was upregulated after neural differentiation and Wnt3 expression level was significantly decreased (Figures 1 and 2). It means that noncanonical Wnt pathway, especially Wnt5a, promotes neural differentiation of ADSCs. Additionally, we have evaluated the protein levels of GSK3 and -catenin and found that protein levels were not changed after neural induction of hADSCs. These results depict the fact that the activation of the Wnt/ -catenin signalling pathway, which is a canonical Wnt pathway, is not involved in neural differentiation in our study.
There are two pathways of noncanonical Wnt signalling, Wnt/Ca 2+ , which downregulate PKC or calcium/calmodulindependent kinase II, or Wnt/JNK pathway by stimulated Wnt5a ligand [17,30]. Zhang et al. reported that Wnt5a induced PKC activation and promoted axon differentiation in cultured hippocampal neurons [31]. These evidences demonstrate the Wnt pathways participation in the initial neural differentiation [21]. Interestingly, we also have observed the expression of Wnt5a and phosphorylation/activation of PKC after neural differentiation. In our study, we have shown that Wnt5a-mediated JNK activation is important in neural differentiation of hADSCs ( Figure 3). This Wnt5a-mediated JNK activation is regulated under the control of not only Fzds but also ERK1/2 signalling (Figure 4).
In this study, we have focused on the stem cell differentiation, especially neural differentiation, through the noncanonical Wnt pathway. We have proved that the expression of Wnt5a, which is one of the noncanonical Wnts, gene level was increased after neuronal differentiation in hADSCs and protein level of downstream phosphor-JNK was also increased in the same way. Taken together, these data demonstrate that noncanonical Wnt signalling could activate the neural differentiation in hADSCs through the regulation of Wnt5a and the activation/phosphorylation of JNK pathway.

Conclusions
Wnt5a promotes noncanonical Wnt signalling followed by Fzd3 and Fzd5 expressions when primary hADSCs are differentiated into neural hADSCs in vitro. Future study will contribute to understanding the control of neural differentiation using Wnt signalling.