Stem Cells International
Volume 2019 (2019), Article ID 4081950, 4 pages
https://doi.org/10.1155/2019/4081950

Erratum

Erratum to “Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells”

Thi Minh Xuan Nguyen, Marketa Vegrichtova, Tereza Tlapakova, Magdalena Krulova, and Vladimir Krylov

Department of Cell Biology, Charles University, Faculty of Science, Vinicna 7, Prague 2 128 44, Czech Republic

Correspondence should be addressed to Vladimir Krylov; vkrylov@natur.cuni.cz

Received 17 June 2019; Accepted 18 June 2019; Published 6 August 2019

Copyright © 2019 Thi Minh Xuan Nguyen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

In the article titled “Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells” [1], due to a production error, the horizontal description of Figure 3(c) was missing. Also, Figure 7(b) was incorrectly replaced by Figure 8(b). The correct figures are as follows:

(a)

(b)

(c)

(d)

Figure 3: GSK-3 inhibitor (CHIR99021) stimulates EMT in XtiSC cell culture. XtiSCs were cultured in a growth medium supplemented with 25 ng/ml FGF2 or 3 μM CHIR99021 or 0.1% DMSO as a control. (a) After three-day treatment, the morphological change of XtiSCs from cobblestone shape to a long-rod shape in a medium with CHIR99021 was observed. Scale bar: 100 μm. (b, c) Immunofluorescent staining and RT-PCR analysis showed the downregulation of epithelial markers (cytokeratin, β-catenin at the plasma cell membrane) (b) and the increase of mesenchymal markers (fibronectin, integrin α5β1, Snai1, and twist1) (c, d). Arrows show the expression of β-catenin at the plasma cell membrane. Nuclei stained with DAPI. Scale bar: 20 μm. Results are representative of three biological replicates; , .

(a)

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Figure 7: In vivo differentiation of EMT-induced XtiSCs into cardiomyocytes. (a) Experimental scheme. RFP-positive XtiSCs were cultured in a growth medium supplemented with 3 μM CHIR99021 or 0.1% DMSO as a control for 3-4 days before transplantation into 2-day-old tadpoles. (b) At the 4th, 14th, or 30th day postinjection (dpi), tadpoles were fixed and sectioned for double staining with antibodies against red fluorescent protein and cardiac troponin T labeling cardiomyocytes in the heart. Scale bar: 20 μm. Nuclei stained with DAPI. Results are representative of four biological replicates.

References

T. M. X. Nguyen, M. Vegrichtova, T. Tlapakova, M. Krulova, and V. Krylov, “Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells,” Stem Cells International, vol. 2019, Article ID 8387478, 16 pages, 2019.