Use of Enzymatic Bio-Fenton as a New Approach in Decolorization of Malachite Green

An enzymatic reaction using glucose oxidase was applied for in situ production of hydrogen peroxide for use in simultaneously Fenton's reaction in decolorization of malachite green. It was found that decolorization rate increased by increasing of glucose concentration from 0.2 g/L to 1.5 g/L. Decolorization rate showed different behaviors versus temperature changes. Initial rate of decolorization process was increased by increasing of temperature; after 30 minutes, especially at temperatures above 30°C, the decolorization rate was gradually reduced. The pH value in the reaction media was decreased from natural to about pH = 3 which had synergic effect on the Fenton process by stabilizing of Fe2+ ions.


Introduction
Releasing of chemical wastes into the environment has caused a variety of environmental problems. Some of the important industrial pollutants are dyes. Dyestuffs are vastly used in the textile, cosmetics, paper, leather, plastics, pharmaceutical, and food industries [1,2]. The textile industry accounts approximately two-thirds of total dyestuff market [3], and it is estimated that as much as 2-50% of applied dyestuff may be lost to wastewater which is finally released into environment [4,5]. Dyeing materials are major sources of environmental contamination, especially for water pollution [6]. Therefore, the removal of dyestuffs from waste effluents becomes environmentally important. However, there is no simple and economical method for color removal from textile effluents. Many methods such as ozonation, photooxidation, electrocoagulation, adsorption, activated carbon, froth flotation, reverse osmosis, ion exchange, membrane filtration, and flocculation have been tested for removing of color from textile effluents [7][8][9]. Nevertheless, expensive plant requirements, high operational costs, regeneration problem, secondary pollutants, sensitivity to variations in wastewater input, interference by other wastewater constituents, and residual sludge generation are some technological and economical disadvantages of these methods [10][11][12][13][14].
Malachite green (MG) belongs to triphenyl methane dyes and is the most applicable material in dyeing industry [15]. It is known that MG has damaging effects on ecosystem and its releasing into aqueous streams will affect aquatic life and cause impairing effects in liver, kidney, intestine, and gonads [10,16]. In addition, MG is tumor promoter to human beings and contact of MG with skin causes irritation, redness, and pain [17,18]. Therefore, removing of MG from contaminated effluents is necessary for ecosystem and human health.
Although conventional decolorization processes are not suitable enough, biological methods are attractive alternatives which have received increasing interest owing to their cost, effectiveness, selectivity, capability of complete degradation of organic pollutants, and in situ use [4,[20][21][22]. On the other hand, Fenton's reaction is a well-known and powerful method for oxidation of organic pollutants [23]. In this reaction, ferrous ion reacts with hydrogen peroxide to produce hydroxyl radical through the following equation [24]: The Scientific World Journal Hydroxyl radicals have powerful oxidizing potential (E • = 2.8 V), so they can easily oxidize a wide diversity of organic materials [25]. As have been reported in literatures, Fenton's reaction has been used to treat various wastewaters containing dyestuffs [26][27][28].
In this study, the feasibility of bio-Fenton reaction, which is a novel approach in decolorization of MG, was investigated. A new simple enzymatic reaction, catalyzed by glucose oxidase (GO X ), was applied for in situ production of hydrogen peroxide and simultaneously Fenton's reaction performed for decolorization of the dye. The effects of various factors including substrate concentration, initial dye concentration, and temperature on the efficiency of bio-Fenton reaction in the removing of MG from aqueous solution were investigated. In addition, variation of pH in bio-Fenton process and its effect on MG decolorization were monitored.

Dye Concentration Analysis.
The concentration of MG in sample solutions was determined by measuring its absorbance with spectrophotometer (UV/Vis spectrophotometer WPA light wave S2000) at maximum absorption wavelengths of the dye (λ max = 619 nm). Different standard solutions were used to plot absorbance calibration curve, which is shown in Figure 2.
As has been shown in Figure 3, the absorbance spectrum of reaction solution without MG is different enough from absorbance pick of aquatic solution of MG, so concentration of MG can be measured accurately.
The decolorization efficiency was calculated through where C 0 is the initial concentration of MG (mg/L) and C is the concentration of MG (mg/L) at time t (min). The decolorization rate was also calculated by (3).

Bio-Fenton Process.
In this process, glucose oxidase in presence of glucose was applied for in situ production of hydrogen peroxide through (4) and simultaneously hydroxyl

Effect of Glucose Concentration.
As can be seen in (4)

Effect of Initial MG Concentration.
Effect of initial MG concentration on decolorization process was tested using different initial concentrations of MG (5, 10, 15, 20, and 40 mg/L). This experiment was conducted at glucose concentration of 1 g/L, 1000 U/L enzyme, and 10 mM [Fe 2+ ] 0 at 30 • C. The results have been shown in Figure 5. As can be seen, by increasing the initial MG concentration, decolorization efficacy decreased. But in terms of decolorization rates, which have been reported in Table 1, it is observed that decolorization rate at various periods of time was maximum for initial MG concentration of 40 mg/L and was minimum for 5 mg/L. It is mainly because there are high   Table 2. In higher temperatures, initial decolorization is faster because of acceleration of hydrogen peroxide reaction with Fe 2+ [19]. However, decolorization rate decreased at temperatures above 30 • C after 30 min. This may be due to deactivation of several active sites of the enzyme because of denaturation of protein molecules by heating and therefore decreasing of in-situ hydrogen peroxide production rate.

pH Trend in the Reaction
Media. The variation of pH value was similar in all experiments and has been illustrated in Figure 6. The pH value in the reaction media was decreased from natural to about 3 which had synergic effect on Fenton process by stabilizing of Fe 2+ ions. For determination of pH effect on MG concentration without bio-Fenton reaction, pHs of MG solutions with initial concentration of 15 mg/L were adjusted at 2.5, 3.5, 4.5, and 5.5 values. After 120 minutes decolorization of the samples was negligible.

Conclusion
In this study a new bio-Fenton process, involving enzymatic in-situ generation of H 2 O 2 , was developed for MG decolorization. Influence of several effective parameters on bio-Fenton process was investigated. It was found that bio-Fenton process was an efficient, simpler, and safe method for decolorization of MG. Decolorization of the dye was observed to be up to %78 during 120 min at the dye concentration of 20 mg/L without taking any control on pH.