The Scientific World Journal

Volume 2013 (2013), Article ID 237438, 6 pages

http://dx.doi.org/10.1155/2013/237438

Research Article

Antimicrobial, Antiparasitic, Anti-Inflammatory, and Cytotoxic Activities of Lopezia racemosa

Carla Cruz Paredes,1,2 Paulina Bolívar Balbás,1,2 Anaximandro Gómez-Velasco,¹ Zaida Nelly Juárez,³ Eugenio Sánchez Arreola,² Luis Ricardo Hernández,² and Horacio Bach¹

¹Department of Medicine, Division of Infectious Diseases, University of British Columbia, 410-2660 Oak Street, Vancouver, BC, Canada, V6H 3Z5

²Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Cholula, 72810 Puebla, PUE, Mexico

³Departamento de Ciencias Biológicas, Facultad Biotecnoambiental, Universidad Popular Autónoma del Estado de Puebla, 72410 Puebla, PUE, Mexico

Received 25 April 2013; Accepted 27 May 2013

Academic Editors: V. C. Filho and D. X. Tan

Copyright © 2013 Carla Cruz Paredes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

The present study investigates the potential benefits of the Mexican medicinal plant Lopezia racemosa (Onagraceae). Extracts and fractions from aerial parts of this plant were assessed to determine their antibacterial, antifungal, antiparasitic, anti-inflammatory and cytotoxic activities in vitro. Aerial parts of the plant were extracted with various solvents and fractionated accordingly. Extracts and fractions were tested against a panel of nine bacterial and four fungal species. The antiparasitic activity was tested against Leishmania donovani, whereas the anti-inflammatory activity of the compounds was determined by measuring the secretion of interleukin-6 from human-derived macrophages. The same macrophage cell line was used to investigate the cytotoxicity of the compounds. Various extracts and fractions showed antibacterial, antifungal, antiparasitic, and anti-inflammatory activities. The hexanic fraction HF 11-14b was the most interesting fraction with antimicrobial, and anti-inflammatory activities. The benefit of L. racemosa as a traditional medicinal plant was confirmed as shown by its antibacterial, antifungal and anti-inflammatory activities. To the best of our knowledge, this is the first study reporting the biological activities of L. racemosa, including antiparasitic and anti-inflammatory activities.

1. Introduction

Lopezia racemosa Cav. (Onagraceae; sin. L. mexicana, L. hirsute Jacq.) is a plant whose distribution is restricted mainly to México [1]. In Mexican folk medicine, L. racemosa has been traditionally used to alleviate stomachache [2], anginas, skin infections, tooth infection, stomach cancer, biliary colic, urine retention [3], and urinary tract infection [3–5].

Chemical profiles from L. racemosa have not yet been reported, but diverse polyphenols (e.g., tannins and flavonoids) and sterols have been isolated from the Onagraceae family [6–8]. The main tannin isolated from this family, oenothein B, has both in vivo and in vitro antitumor activities [9–12]. Among flavonoids, isolated compounds from this family include flavonols, glycoflavones, flavones, and chalcones [13, 14]. Most of the genera studied possess mainly flavonols, which include myricetin, quercetin, kaempferol, and remarkable amounts of various phenolic acids [6, 7, 15]. Furthermore, some species produce significant amount of tocopherols, compounds that have been used as chemotaxonomic markers within the Onagraceae family [16].

Despite its widespread use in México, there is no scientific evidence that supports the abovementioned traditional use. Thus, in the present study, we investigate the antibacterial, antifungal, and antiparasitic activities of L. racemosa as well as exploring its cytotoxic and anti-inflammatory effects on human macrophages.

2. Materials and Methods

2.1. Plant Material

L. racemosa was collected at 2476 m above sea level in San Nicolás de los Ranchos, Puebla, México (19°04′03′′ N and 98°29′79′′ W). A voucher specimen numbered 14469 was deposited in the Herbarium of the Benemérita Universidad Autónoma de Puebla, México.

2.2. Preparation of Plant Extracts

Extracts from 135 g of air-dried aerial parts from L. racemosa were extracted sequentially with the following solvents: n-hexane (hexane), chloroform, and methanol. Three rounds of extraction were conducted for each solvent over a period of 48 h. Extracted materials were filtered and the solvents volatilized in vacuo at 40°C. The hexane, chloroform, and methanol extracts (HE, CE, and ME) yielded 0.67%, 0.97%, and 24.65% of residue, respectively. The extracts were chromatographed using silica gel (70–240 mesh) and 190 mL of pure or combined solvents. A total of 115 fractions corresponding to all of the three extracts were collected and combined according to their TLC profile, generating five hexanic fractions, four chloroformic fractions, and six methanolic fractions, which were further analyzed for their bioactivity properties. Fractions were dried in a vacuum using a rotary evaporator at 40°C. Stock solutions were prepared by dissolving 20 mg of each extract or fraction in 100 μL DMSO, following sonication (Branson 3210) for 60 min at 30°C until the material was dissolved. pH values varying between 6.5 and 7.3 were measured after stock preparation of extracts and fractions.

2.3. Strains and Culture Media

The following Gram-negative strains Acinetobacter baumannii (ATCC BAA-747), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC14210), and Salmonella typhimurium (ATCC 13311) were assayed. The Gram-positive strains included Bacillus subtilis (ATCC 6633), Staphylococcus aureus (ATCC 25923), methicillin-resistant Staphylococcus aureus (MRSA) (ATCC 700698), and Streptococcus pyogenes (ATCC 51878). The acid-alcohol-fast Mycobacterium smegmatis mc²155 (ATCC 700084) was also included in the panel of bacteria to be tested. The filamentous fungi Aspergillus fumigatus (ATCC 1022) and Trichophyton rubrum (ATCC 18758), the yeast Candida albicans (provided by Vancouver General Hospital, British Columbia, Canada), and Cryptococcus neoformans var. grubii (kindly provided by Dr. Karen Bartlett, University of British Columbia, BC, Canada) were tested as representatives of pathogenic fungi. The parasite Leishmania donovani Sudan strain 2S was assessed for antiparasitic activity of the extracts and fractions and was kindly provided by Dr. Neil Reiner (University of British Columbia,Vancouver, BC, Canada).

Bacterial strains were cultured in Mueller-Hinton medium (Becton and Dickinson) except for Mycobacterium smegmatis, which was cultured in Trypticase Soy broth (Becton and Dickinson) supplemented with 0.05% Tween-80 (Fisher). Bacterial stocks were maintained on the same medium supplemented with 1.5% agar (Becton and Dickinson) at 4°C. All the bacterial strains were cultured at 37°C. For fungal strains, Sabouraud broth (Becton and Dickinson) was used. In the case of filamentous fungi, bioactivities were tested using harvested spores. Spores of Aspergillus fumigatus and Trichophyton rubrum were carefully harvested and stored as published [17]. Both strains of filamentous fungi were incubated at 28°C. For Candida albicans and Cryptococcus neoformans, the same protocol used for bacterial strains was followed, but with Sabouraud broth. Leishmania donovani promastigotes were cultured as published [17].

2.4. Antimicrobial and Antiparasitic Assays

A microdilution assay was used to determine the antimicrobial activities in a 96-well plate and according to published protocols [17]. Briefly, bacterial strains were grown overnight by shaking (200 rpm) at 37°C, and the bacterial densities were adjusted to an optical density of 0.05 at 625 nm. Extracts and fractions at concentrations of 10, 40, 100, 200, 400, 500, and 1000 μg/mL were evaluated in a final volume of 200 μL/well. Organisms were incubated for 24 h at 37°C with the exception of fungi, which were cultured at 28°C. The minimal inhibitory concentrations (MICs) were determined when no turbidity in the well was observed. DMSO and untreated inocula were used as negative controls, while amikacin, ampicillin, gentamicin, and rifampicin were used as positive controls. Antifungal activities were assessed in Sabouraud broth (BD) and strains were grown at 28°C for 72 h using the same format of 96-well plates. In the case of T. rubrum and A. fumigatus, cultures were started from harvested spores as reported in [17]. DMSO and untreated inocula were used as negative controls, while amphotericin B was used as a positive control.

The evaluation of antiparasitic activity was performed in 24-well flat bottom plates containing promastigotes/well. Compounds were evaluated at the final concentrations mentioned for the antibacterial activities. Untreated parasites and DMSO were used as negative controls. Motility and number of parasites were annotated at 24, 48, and 72 h after treatment and after staining the sample with 0.4% Trypan blue solution.

2.5. Cytotoxic Assay

Human-derived THP-1 monocytic cells (ATCC 202) were cultured in RPMI 1640 (Sigma-Aldrich) supplemented with 5% fetal calf serum (FCS) (Sigma-Aldrich) and 2 mM L-glutamine (STEMCELL Technologies, Vancouver, Canada). THP-1 cells were diluted to a final concentration of  cell/well in a 96-well plate. Plates were placed in a humidified atmosphere at 37°C supplemented with 5% CO₂ for 24 h. Untreated THP-1 cells and DMSO were used as negative controls, whereas 5% hydrogen peroxide was used as a positive control. Propidium iodide (PI) was used to evaluate membrane damage [18]. The half maximal inhibitory concentration (IC₅₀) was calculated by plotting the compound concentrations against the percentage of damaged cells.

2.6. Anti-Inflammatory Assay

THP-1 cells at concentrations of cells/well were dispensed in a 96-well plate and activated with 80 ng/mL phorbol myristate acetate (Sigma-Aldrich). Plates were incubated at 37°C in a humidified atmosphere supplemented with 5% CO₂ for 24 h. Then, differentiated cells were gently washed with fresh medium (3X) and incubated in presence of the compounds for 6 h. After this time, cells were gently washed again with fresh medium (3X) and an inflammatory response was initiated upon the addition of 100 ng/mL of lipopolysaccharide (LPS) from E. coli (Sigma-Aldrich). After 3 h, cells were pelleted by centrifugation at 1000 rpm for 5 min and the supernatants were transferred to a new 96-well plate and kept at −20°C for further analysis. Interleukin-6 (IL-6) (R&D) was used to evaluate the anti-inflammatory activity using a sandwich ELISA. Briefly, 96-well plates were coated with 20 μg human IL-6 overnight at 4°C. The next day, plates were washed with phosphate buffered saline (PBS), supplemented with 0.05% Tween-20 (PBS-T), and blocked with 3% bovine serum albumin (BSA, Sigma-Aldrich) dissolved in PBS overnight at 4°C. BSA was washed away with PBS-T (3X) and 50 μL of each supernatant was added. After 2 h incubation, wells were washed again with PBS-T (3X) and exposed to biotinylated anti-human IL-6 (R&D) according to manufacturer’s instructions. After 1 h incubation, wells were washed again with PBS-T (3X) and avidin-HRP (BD Opt EIA) diluted 1 : 250 in 3% BSA was added. After a further 1 h incubation, wells were washed again with PBS-T (3X) and the presence of IL-6 was evaluated by the addition of 50 μL 3,3′,5,5′-tetramethylbenzidine until a blue colour developed. Reactions were stopped by the addition of 25 μL of 1 M sulfuric acid solution. Absorbance was read in an ELISA reader (Bio-Rad) at 450 nm. THP-1 cells exposed to DMSO or LPS were used as negative controls, while dexamethasone was used as a positive control. Values are expressed as percentage of inflammation after normalization to LPS.

2.7. Statistical Analysis

A Student’s -test was used for statistical analysis. A value <0.05 was considered significant.

3. Results

3.1. Antimicrobial Activities

Extracts and fractions obtained from L. racemosa were tested against a panel of Gram-negative and -positive bacteria. Four different fractions showed significant activity in inhibiting the growth of bacteria at concentration ranging from 40 to 400 μg/mL (Table 1). Fractions HF 11–14b and MF 28–36 and the extract CE prevented the growth of MRSA at concentrations of 40 μg/mL, whereas the fraction HF 16 inhibited the growth of S. aureus at the same concentration (Table 1).

Table 1: Antimicrobial activity of extracts and fractions of Lopezia racemosa expressed as MIC (g/mL).

Bacteria

Fungi

AB
BS
MRSA
MS
SA
SP
CA
CN
TR

HE
NA
NA
NA
NA
400
NA
NA
NA
NA

HF 9-10
NA
NA
400
NA
NA
NA
NA
NA
NA

HF 11–14b
NA
NA
40
NA
NA
NA
NA
NA
NA

HF 16
NA
400
400
NA
40
400
400
NA
NA

HF 18-19
NA
400
NA
NA
NA
400
NA
NA
NA

CE
400
400
40
NA
NA
NA
NA
NA
NA

CF 37–40
NA
NA
NA
400
400
NA
NA
NA
NA

CF 48–50
NA
NA
NA
NA
NA
NA
NA
NA
10

ME
NA
NA
NA
NA
NA
400
NA
NA
NA

MF 17
NA
400
400
NA
400
NA
400
400
NA

MF 20–24
NA
NA
400
NA
400
NA
NA
NA
NA

MF 28–36
NA
NA
40
400
NA
NA
NA
NA
40

Reference
0.1 (Ak)
15 (Ak)
60 (G)
0.7 (R)
1 (G)
(0.7) (Ap)
2 (A)
2 (A)
ND

AB: Acinetobacter baumannii; BS: Bacillus subtilis; MRSA: methicillin-resistant Staphylococcus aureus; MS: Mycobacterium smegmatis; SA: Staphylococcus aureus; SP: Streptococcus pyogenes; CA: Candida albicans; CN: Cryptococcus neoformans; TR: Trichophyton rubrum. NA: no activity detected; HE: hexane extract; CE: chloroform extract; ME: methanol extract; HF: hexanic fraction; CF: chloroformic fraction; MF: methanolic fraction. Experiments were performed in triplicate. A: amphotericin; Ak: amikacin; Ap: ampicillin; G: gentamicin; R: rifampicin; ND: no determined.

The antifungal activity of L. racemosa was also investigated against four strains of human pathogenic fungi. Only the fractions CF 48–50 and MF 28–36 were highly active against T. rubrum at concentration as low as 10 and 40 μg/mL, respectively (Table 1), whereas the fraction MF 17 inhibited the growth of both yeasts C. albicans and C. neoformans at concentrations of 400 μg/mL.

3.2. Antiparasitic Activity

Extracts and fractions were also tested against the parasite L. donovani. The fractions HF 11–14b and MF 28–36 and the extract CE significantly reduced the number of L. donovani parasites 72 h after infection, compared to the nontreated cells, by approximately 88% (Figure 1).

Figure 1: Antiparasitic activity exhibited by fractions of Lopezia racemosa. Leishmania donovani promastigote growth inhibition was evaluated after incubation of the parasites with extracts and fractions. Untreated promastigotes and DMSO were used as negative controls. Shown is the mean ± SE of three independent experiments. value < 0.05. CE, chloroform extract; HF and MF represent hexanic and methanolic fractions, respectively.

3.3. Cytotoxic and Anti-Inflammatory Activities

We assessed the cytotoxic and anti-inflammatory activities of the fractions and extracts that showed either antibacterial, antifungal, or antiparasitic activities. Cytotoxicity was evaluated with human-derived macrophages (THP-1 cells). The majority of extracts showed cytotoxic effects above 50% (Figure 2 and Table 2). Only the fraction MF 28–36 showed no significant cytotoxicity (below 25%) with IC₅₀ of approximately 770 μg/mL.

Table 2: Half-maximal inhibitory concentrations (IC₅₀) of L. racemosa extracts and fractions expressed in g/mL.

Extract
IC₅₀ (SD)
Fraction
IC₅₀ (SD)

HE
30.66 6.3
HF 9-10
49.98 5.1

HF 11–14a
70.02 9.5

HF 16
34.3 6.9

HF 18-19
86.93 12.4

CE
28.58 3.6
CF 37–40
86.96 12.0

CF 48–50
122.3 2.4

ME
29.87 7.7
MF 4–16
25.62 4.9

MF 17
33.87 3.1

MF 20–24
40.3 6.3

MF 28–36
769.54 98.6

Figure 2: Cytotoxic effects of extracts and fractions of Lopezia racemosa. Human macrophages (THP-1 cells) were used to assess the cytotoxic effects of bioactive compounds using propidium iodide staining. Dashed lines represent treatment with 5% H₂O₂ (upper line) (positive control), and untreated cells (lower line) (negative control). Shown is the mean ± SE of three independent experiments. value < 0.05. HE, CE, and ME are hexane, chloroform, and methanol extracts, respectively. HF, CF, and MF represent hexanic, chloroformic, and methanolic fractions, respectively.

The anti-inflammatory activity was assessed by measuring the secretion of IL-6 from macrophages exposed to fractions and extracts. Most of fractions did not possess any anti-inflammatory activity, but fraction HF 11–14b showed significant anti-inflammatory activity by reducing the secretion of IL-6 by approximately 50% when an immunological response was generated by exposure of the cells to LPS (Figure 3).

Figure 3: Anti-inflammatory activity of extracts and fractions from Lopezia racemosa. An inflammatory process was elicited by exposing macrophages to LPS. Extracts and fractions were exposed overnight to macrophages prior to the addition of LPS. The anti-inflammatory process was evaluated by measuring the secretion of IL-6 into the supernatant. Shown is the mean ± SE of three independent experiments. value < 0.05. HF, methanolic fraction.

4. Discussion

In the present study, we examine the biological properties of L. racemosa, which has been traditionally used to treat diverse medical conditions such as skin and urinary tract infections as well as stomach cancer. To the best of our knowledge, we provide the first experimental evidence demonstrating its antimicrobial activities against bacteria, fungi, and a parasite.

Antibacterial activities were found only against the Gram-positive bacteria, MRSA and S. aureus. Unfortunately, results from this study cannot be compared to the literature because of the lack of reports about the genus Lopezia. However, extracts from different members of the Onagraceae family, such as Epilobium and Ludwigia, have broad activity against Gram-positive and -negative bacteria [19–22].

Two fractions of L. racemosa showed significant antifungal activity against the dermatophyte T. rubrum. In agreement with our study, the extract of E. angustifolium has been used clinically to treat tinea capitis, a dermatophytosis of the scalp, which is caused either by Microsporum or Trichophyton [23]. Several studies have also shown that extracts from E. angustifolium were active against a broad range of fungi including several strains of Candida, C. neoformans, Saccharomyces cerevisiae, Microscoporum canis, and the saprobic fungus Rhizopus sp. [20, 22, 24, 25]. On the other hand, ethanol extract of Oenothera biennis was highly active against the phytopathogen Fusarium semitectum [26].

In this study, we report for the first time that two fractions (HF 11–14b and MF 28–36) and one extract (CE) showed significant antiparasitic activity against L. donovani.

Despite their antimicrobial activities, fraction MF 28–36 was not toxic to macrophages. Moreover, a single fraction HF 11–14b showed significant anti-inflammatory activity. Diverse studies have demonstrated that different members of the Onagraceae family have selective anti-inflammatory activity in animal models [7, 8, 15, 27] and cytotoxic activities against several tumor cell lines [11, 12, 28–30].

It is remarkable that extracts of the members of the Onagraceae family have diverse biological effects. These properties are due to the high content of polyphenols, such as tannins and flavonoids. Phytochemical analyses have shown that oenothein B is the main tannin isolated from Epilobium and Oenothera, followed by other flavonoids such as myricetin-, quercetin-, and kaempferol-glycosides [6–8]. Due to its high abundance, oenothein B is considered the main compound responsible for the antitumor, antioxidant, antibacterial, antiviral, and phagocytic activities both in vivo and in vitro [9–11, 31]. Yet, other compounds present in the extracts may exert different biological activities as observed in different studies. In this regard, extracts of L. racemosa may have similar active compounds.

5. Conclusions

In summary, we have demonstrated for the first time that extracts of the aerial parts of L. racemosa exert antibacterial, antifungal, antiparasitic, anti-inflammatory, and cytotoxic activities. The fraction HF 11–14b deserves further study to elucidate its specific biological properties and identify the compound(s) responsible for its antimicrobial and anti-inflammatory activities.

Acknowledgment

The authors thank Jeffrey Helm for the helpful discussions.

References

W. L. Wagner, P. C. Hoch, and P. H. Raven, Revised Classification of the Onagraceae, The American Society of Plant Taxonomists, Ann Arbor, Mich, USA, 2007.

M. González Elizondo, I. L. López Enriquez, M. S. González Elizondo, and J. A. Tena Flores, Plantas Medicinales del Estado de Durango y Zonas Aledañas, Instituto Politécnico Nacional-PROSIMA, Mexico City, México, 2004.

A. Aguilar, J. R. Camacho, S. Chino, P. Jácquez, and M. E. López, Herbario Medicinal Del Instituto Mexicano Del Seguro Social, IMSS, 1994.

A. J. Alonso-Castro, M. L. Villarreal, L. A. Salazar-Olivo, M. Gomez-Sanchez, F. Dominguez, and A. Garcia-Carranca, “Mexican medicinal plants used for cancer treatment: pharmacological, phytochemical and ethnobotanical studies,” Journal of Ethnopharmacology, vol. 133, no. 3, pp. 945–972, 2011.

BDMTM, “Biblioteca Digital de la Medicina Tradicional Mexicana,” 2012, http://www.medicinatradicionalmexicana.unam.mx/

B. Ducrey, J. L. Wolfender, A. Marston, and K. Hostettmann, “Analysis of flavonol glycosides of thirteen Epilobium species (Onagraceae) by LC-UV and thermospray LC-MS,” Phytochemistry, vol. 38, no. 1, pp. 129–137, 1995.

B. T. Hevesi, P. J. Houghton, S. Habtemariam, and Á. Kéry, “Antioxidant and antiinflammatory effect of Epilobium parviflorum Schreb,” Phytotherapy Research, vol. 23, no. 5, pp. 719–724, 2009.

A. K. Kiss, A. Bazylko, A. Filipek et al., “Oenothein B's contribution to the anti-inflammatory and antioxidant activity of Epilobium sp,” Phytomedicine, vol. 18, no. 7, pp. 557–560, 2011.

D. Lesuisse, J. Berjonneau, C. Ciot et al., “Determination of oenothein B as the active 5-α-reductase-inhibiting principle of the folk medicine Epilobium parviflorum,” Journal of Natural Products, vol. 59, no. 5, pp. 490–492, 1996.

K. Miyamoto, M. Nomura, M. Sasakura et al., “Antitumor activity of oenothein B, a unique macrocyclic ellagitannin,” Japanese Journal of Cancer Research, vol. 84, no. 1, pp. 99–103, 1993.

A. Vitalone, M. Guizzetti, L. G. Costa, and B. Tita, “Extracts of various species of Epilobium inhibit proliferation of human prostate cells,” Journal of Pharmacy and Pharmacology, vol. 55, no. 5, pp. 683–690, 2003.

A. Vitalone, J. McColl, D. Thome, L. G. Costa, and B. Tita, “Characterization of the effect of Epilobium extracts on human cell proliferation,” Pharmacology, vol. 69, no. 2, pp. 79–87, 2003.

J. E. Averett and P. H. Raven, “Flavonoids of onagraceae,” Annals of the Missouri Botanical Garden, vol. 71, pp. 30–34, 1984.

W. A. Dement and P. H. Raven, “Distribution of the chalcone, isosalipurposide, in the onagraceae,” Phytochemistry, vol. 12, no. 4, pp. 807–808, 1973.

M. S. Marzouk, F. A. Moharram, R. A. El Dib, S. M. El-Shenawy, and A. F. Tawfike, “Polyphenolic profile and bioactivity study of Oenothera speciosa nutt. aerial parts,” Molecules, vol. 14, no. 4, pp. 1456–1467, 2009.

L. Velasco and F. D. Goffman, “Tocopherol and fatty acid composition of twenty-five species of Onagraceae Juss,” Botanical Journal of the Linnean Society, vol. 129, no. 4, pp. 359–366, 1999.

P. Bolivar, C. Cruz-Paredes, L. R. Hernández et al., “Antimicrobial, anti-inflammatory, antiparasitic, and cytotoxic activities of Galium mexicanum,” Journal of Ethnopharmacology, vol. 137, no. 1, pp. 141–147, 2011.

M. G. Martínez Ruiz, M. Richard-Greenblatt, Z. N. Juárez, Y. Av-Gay, H. Bach, and L. R. Hernández, “Antimicrobial, anti-inflammatory, antiparasitic, and cytotoxic activities of Laennecia confusa,” Scientific World Journal, vol. 2012, Article ID 263572, 2012.

W. J. Bartfay, E. Bartfay, and J. G. Johnson, “Gram-negative and gram-positive antibacterial properties of the whole plant extract of willow herb (Epilobium angustifolium),” Biological Research for Nursing, vol. 14, no. 1, pp. 85–89, 2012.

L. Battinelli, B. Tita, M. G. Evandri, and G. Mazzanti, “Antimicrobial activity of Epilobium spp. extracts,” Farmaco, vol. 56, no. 5–7, pp. 345–348, 2001.

O. Oyedeji, M. Oziegbe, and F. O. Taiwo, “Antibacterial, antifungal and phytochemical analysis of crude extracts from the leaves of Ludwigia abyssinica A. Rich. and Ludwigia decurrens walter,” Journal of Medicinal Plant Research, vol. 5, no. 7, pp. 1192–1199, 2011.

J.-P. Rauha, S. Remes, M. Heinonen et al., “Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds,” International Journal of Food Microbiology, vol. 56, no. 1, pp. 3–12, 2000.

A. Kiliç, A. Kiliç, and T. Inandi, “Folkloric treatment of tinea capitis with Epilobium angustifolium,” Plastic and Reconstructive Surgery, vol. 108, no. 6, pp. 1824–1825, 2001.

N. P. Jones, J. T. Arnason, M. Abou-Zaid, K. Akpagana, P. Sanchez-Vindas, and M. L. Smith, “Antifungal activity of extracts from medicinal plants used by First Nations Peoples of eastern Canada,” Journal of Ethnopharmacology, vol. 73, no. 1-2, pp. 191–198, 2000.

D. Webster, P. Taschereau, R. J. Belland, C. Sand, and R. P. Rennie, “Antifungal activity of medicinal plant extracts; preliminary screening studies,” Journal of Ethnopharmacology, vol. 115, no. 1, pp. 140–146, 2008.

Y. N. Shukla, A. Srivastava, S. Kumar, and S. Kumar, “Phytotoxic and antimicrobial constituents of Argyreia speciosa and Oenothera biennis,” Journal of Ethnopharmacology, vol. 67, no. 2, pp. 241–245, 1999.

M. Meckes, A. D. David-Rivera, V. Nava-Aguilar, and A. Jimenez, “Activity of some Mexican medicinal plant extracts on carrageenan-induced rat paw edema,” Phytomedicine, vol. 11, no. 5, pp. 446–451, 2004.

T. Arimura, A. Kojima-Yuasa, M. Suzuki, D. O. Kennedy, and I. Matsui-Yuasa, “Caspase-independent apoptosis induced by evening primrose extract in Ehrlich ascites tumor cells,” Cancer Letters, vol. 201, no. 1, pp. 9–16, 2003.

E. Jaszewska, A. Kosmider, A. K. Kiss, and M. Naruszewicz, “Oenothera paradoxa defatted seeds extract containing pentagalloylglucose and procyanidins potentiates the cytotoxicity of vincristine,” Journal of Physiology and Pharmacology, vol. 61, no. 5, pp. 637–643, 2010.

C. Dalla Pellegrina, G. Padovani, F. Mainente et al., “Anti-tumour potential of a gallic acid-containing phenolic fraction from Oenothera biennis,” Cancer Letters, vol. 226, no. 1, pp. 17–25, 2005.

I. A. Schepetkin, L. N. Kirpotina, L. Jakiw et al., “Immunomodulatory activity of oenothein B isolated from Epilobium angustifolium,” Journal of Immunology, vol. 183, no. 10, pp. 6754–6766, 2009.