The Scientific World Journal
Volume 2014 (2014), Article ID 127526, 10 pages
http://dx.doi.org/10.1155/2014/127526

Research Article

Ethnoveterinary Study of Medicinal Plants in a Tribal Society of Sulaiman Range

Akash Tariq,¹ Sakina Mussarat,¹ Muhammad Adnan,¹ Naser M. AbdElsalam,² Riaz Ullah,³ and Abdul Latif Khan⁴

¹Department of Botany, Kohat University of Science and Technology, Kohat 26000, Pakistan
²Riyadh Community College, King Saud University, Riyadh 11437, Saudi Arabia
³Department of Chemistry, Government College Ara Khel, FR Kohat 26000, Pakistan
⁴Department of Biological Sciences and Chemistry, University of Nizwa, 616 Nizwa, Oman

Received 24 July 2014; Accepted 24 September 2014; Published 21 October 2014

Academic Editor: Michael J. Myers

Copyright © 2014 Akash Tariq et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

The aims of the present study were (i) to document ethnoveterinary plants and their formulation techniques in an unexplored region of Pakistan and (ii) to select candidate medicinal plants with high consensus factor and fidelity value for further in vitro investigation. A total of 60 informants were interviewed using semistructured questionnaire. A total of 41 plants belonging to 30 families were used to treat livestock ailments in study area. Mostly leaves (47%) were used in recipes formulation mostly in the form of decoction. Gastrointestinal infections were found more common and majority of the plants were used against cow (31) and buffaloes (24) ailments. Recovery time of majority of the recipes was three to four days. Informant consensus factor (Fic) results have shown a high degree of consensus for gastrointestinal, respiratory, and reproductive (0.95 each) ailments. Fidelity level (FL) results showed that Asparagus gracilis ranked first with FL value 93% followed by Rumex hastatus ranked second (91%) and Tinospora cordifolia ranked third (90%). Aged farmers and nomads had more traditional knowledge as compared to younger ones. Plants with high Fic and FL values could be further investigated in vitro for the search of some novel bioactive compounds and young generation should be educated regarding ethnoveterinary practices.

1. Introduction

Traditional veterinary medicine was experienced as early as 1800 B.C. at the time of King Hammurabi of Babylon who devised laws on veterinary fees and charged for treating animals [1]. Many ethnoveterinary medicines were neglected due to the development of initial western drugs. Ethnoveterinary practices have gained tremendous importance for the last decade due to the discovery of some effective ethnoveterinary products [2]. Traditional veterinary medicines provide a cheap therapy and easy accessibility in comparison with western drugs [3].

Ethnoveterinary practices are more common in developing countries due to different socioeconomic factors [4]. Pakistan is an agriculture country and almost 80% of its population is dependent on agriculture and livestock. Pakistan is the world’s 5th largest milk producing country due to its high dependency on agriculture and livestock [5]. Resource-poor farmers of Pakistan greatly rely on traditional medicine due to their limited access to modern prevention health practices and particularly lack of modern health facilities in their areas [6]. Despite the fact that traditional knowledge is very much important for the livestock health and productivity, the documentation of this knowledge is very much neglected in majority of the remote areas of Pakistan. Livestock farmers all over Pakistan can draw on over 4000 years of knowledge and experience [7]. This traditional knowledge has been passed orally from generation to generation but it may be extinct or may be endangered due to the current rapid socioeconomic, environmental, and technological changes [8]. Therefore, the documentation of such knowledge is very crucial before its extinction for future developments.

The present research study was therefore designed to document detailed ethnoveterinary knowledge of an unexplored region of Pakistan. The present study was designed with the objectives (i) to document ethnoveterinary plants of the Kohat region, (ii) to document the detailed formulation techniques of the reported ethnoveterinary plants, and (iii) to select candidate medicinal plants with high consensus factor and fidelity value for further in vitro investigation. The present study would provide baseline information to phytochemists, pharmacologists, and conservationists for further future research studies. This work would also make a great contribution to the conservation of this valuable knowledge.

2. Methodology

2.1. Study Area

Kohat is a medium sized town in Khyber Pakhtunkhwa of Pakistan. It is located at 33°35′13 N, 71°26′29 E, with an altitude of 489 m, and is the capital of the Kohat District (Figure 1). Summer temperatures usually shoot above 50°C and winters are mild [9]. The dominant vegetation of the study area is Zizyphus species, Acacia species, and other xerophytes plants. The area is rural in nature and inhabitants are very much dependent on livestock for agricultural, economic, and food purposes. Locals of the region use a variety of medicinal plants for the treatment of livestock ailments due to expensive veterinary drugs.

Figure 1: Study area map.

2.2. Sampling and Data Collection

Field work was done from January to May 2014. Initially, local administrative officers and representative (Malik) of the study area were visited, who provided information on key resource persons in the field of ethnoveterinary medicinal plants. They suggested 60 informants having strong traditional knowledge regarding livestock treatment. Out of 60 informants 45 were farmers and 15 were nomadic people. A brief group discussion was held with the informants prior to data collection for explaining to them the main theme of the present study and to get their consent for the publication of their traditional knowledge. This was done in order to acknowledge informants’ cooperation in preserving the traditional knowledge of the study area and build their confidence for providing reliable information. Each informant was separately interviewed in their local languages. Semistructured questionnaires were designed addressing detailed ethnoveterinary information (Hindko). Informants were asked about the number of plants they use to treat their livestock, which part of plant used, recipe formulation, recovery, and other essential questions.

2.3. Data Organization

Data collected from informants was organized using Microsoft Excel 2007 and Microsoft Word 2007. Plant habit was categorized into four classes, that is, herb, shrub, tree, and climbers. Plant parts were classified into leaves, stem, root, stem, whole plant, seeds, and fruit. Medicinal plants uses were categorized into 8 major categories, that is, gastrointestinal, dermatological, respiratory, reproductive, wound healing, antipyretic, parasitic, and general body tonic. Recipes were classified into different groups, that is, decoction, powder, crushed, juice, paste, poultice, infusion, and concoction. Route of administration was divided into 3 categories, that is, oral, dermal, and nasal.

2.4. Data Analysis

Informant consensus and fidelity level were used to verify the importance of medicinal plants.

2.5. Informant Consensus (Fic)

Fic on the reported cures of a given group of ailments was calculated as an informant consensus factor. Fic within a community designates the widely used plants and thus helps in the selection of plants for phytochemical and pharmacological studies [10]. Reported veterinary problems were grouped into 8 major ailments. Fic values are high when one or few plants are reported by the large number of respondents to treat a specific ailment, while low Fic values give an indication that informants do not agree over which plant to use [11, 12].

The Fic can be calculated using the formula as follows:

where Fic = Informants consensus factor, nur = number of used citations in each category, and nt = number of species used.

2.6. Fidelity Level (FL)

FL is useful for recognizing the most preferred plants used for curing certain ailments by the respondents. Highly preferred plants have always greater FL values than those that are less preferred. FL is always calculated in terms of percentage of informants claiming the use of a certain plant species for the same major purpose. The main purpose of FL is to calculate the importance of plant species for a specific purpose. Prior to the calculation of FL values all of the ailments that were reported are grouped into major classes [10]. FL value was estimated using the formula FL = Ip/Iu × 100, where Ip is the number of respondents who reported the utilization of medicinal plants for a specific main ailment and Iu is the total number of respondents who mentioned the same plant for any ailment [13]. It is assumed that those medicinal plants which are plants used in some recurring manner for the same disease category are more likely to be biologically active [14].

2.7. Collection and Preservation of the Reported Medicinal Plants

Field trips were made with local informants for the collection of the reported medicinal plants. Collected medicinal plants were brought to the laboratory of Kohat University of Science and Technology (KUST), Kohat, Pakistan, for further processing. Specimen identification and confirmation were undertaken by using Flora of Pakistan and taxonomic experts. Plants were dried and pressed on herbarium sheets and deposited at the Herbarium of Department of Botany, KUST, Kohat, Pakistan.

3. Results

The present study revealed that local farmers of Kohat region utilize 41 medicinal plants belonging to 30 families for the treatment of different types of livestock ailments (Table 1). Among all the families, Asteraceae was found to be dominant (4 species) in the study area being in use in ethnoveterinary practices in the region. Traditional farmers mostly used herbs (55%) for the preparation of ethnomedicines (Table 2) followed by shrubs (27%). Almost all plant parts were being used for medicinal purposes but leaves (47%) were found to be the most frequently used plant part followed by whole plant (32%) and roots (17%) (Table 2). Local farmers used these ethnomedicines to treat different types of domestic animals like buffaloes, cows, goats, sheep, and donkeys. A total of 31 plants were found to be used for treatment of cow ailments followed by 24 plants against buffalo’s ailments and 17 for goats (Figure 2). Different types of ailments were treated which were categorized into 8 major categories. Gastrointestinal infections were found to be most common in domestic animals and a total of 13 plants were used against them followed by 7 plants which are used as antipyretic while 6 are used for wounds treatment (Table 3). Local farmers prepare different types of ethnomedicines but the most preferred techniques were decoction and powder (10 plants each) followed by crushing (7 plants) in the studied region (Figure 3). Monotherapy was most common in the study area; only few plants were found to be used in concoction form (Table 1). For example, stem of Allium sativum is mixed with flower of Punica granatum and milk and used against gastrointestinal infection; roots of Asparagus gracilis are mixed with leaves of Coriandrum sativum to make fine concoction and given with water to cattle for delivery purposes. Different types of vehicles were found to be used for preparation and administration of plant recipes like sugar, flour, water, and milk (Table 1). The most common route of administration was oral (75%) followed by dermal (24%) and only single species is administered through nasal pathway (Table 1). Recovery time of majority of the recipes was three to four days. Informant consensus (Fic) results have shown a high degree of consensus for gastrointestinal, respiratory, and reproductive (0.95 each) ailments, which were followed by parasitic infections and wound healing (0.90 each) (Table 3). The highest plant use citation was for gastrointestinal (260) followed by wound healing (53) and reproductive (47) ailments. The present study revealed 10 medicinal plants having high FL value (Table 4). FL values in this study varied from 1.0% to 100%. Asparagus gracilis ranked first with the highest FL value (93%) followed by Rumex hastatus ranked second (91%), Tinospora cordifolia ranked third (90%), and Aloe barbadensis ranked fourth (85%). The entire informants interviewed were aged people ranging between 40 and 70 years old. No use of ethnoveterinary medicine by women or young generation was recorded.

Table 1: Ethnoveterinary plants used for the treatment of livestock ailments in Kohat region.

Plant names/families name/voucher number
Local names
Habit
Part used
Ailment treated
Animal treated
Recipe
Vehicles
Route
Recovery

Acacia modesta Wall. Fabaceae
KUH-421
Kikar
Tree
Leaves, seeds
Delivery
Buffaloes, cow
Decoction
Water
Oral
Two days

Achyranthes aspera Linn. Amaranthaceae
KUH-422
Gishkay
Herb
Whole plant
Anthelmintic and delivery
Buffaloes, cow, goat, sheep
Powder
Sugar
Oral
One day

Allium cepa L. Amaryllidaceae
KUH-423
Pyaz
Herb
Whole plant
Febrifuge, tonic
Sheep, goat
Decoction
Flour
Oral
Three days

Allium sativum L. Liliaceae
KUH-424
Thoom
Herb
Stem
Gastrointestinal
Goat
Concoction
Milk
Oral
Ten days

Aloe barbadensis Mill. Liliaceae
KUH-425
Kunwar
Shrub
Root
Gastrointestinal
Sheep, cow, buffaloes
Powder
Milk
Oral
Six days

Artemisia brevifolia Wall. Asteraceae
KUH-426
Jaukay
Herb
Leaves
Removal of placenta
Cow
Decoction
Sugar
Oral

Asparagus gracilis Royle. Liliaceae
KUH-427
Lachgawa
Herb
Root
Delivery
Goat
Concoction
Water
Oral

Brassica campestris L. Brassicaceae
KUH-428
Sarson
Herb
Whole plant
External lice (blood feeding)
Cow, buffaloes
Paste

Dermal
Five days

Calotropis procera (Willd.) R.Br. Apocynaceae
KUH-429
Spulmaey
Shrub
Fruit, leaves
Intestinal worms and skin infections
Buffaloes, cow, goat, sheep
Paste, concoction
Sugar
Oral, dermal
Three days

Cannabis sativa L. Cannabaceae
KUH-430
Bhang
Shrub
Leaves
External parasite, appetizer
Cow, donkey, buffaloes
Poultice, powder

Dermal, oral
Two days

Chenopodium album L. Amaranthaceae
KUH-431
Samaray
Herb
Whole plant
Wound healing
Goat, sheep, cow
Paste

Dermal
One day

Chrysanthemum leucanthemum L. Asteraceae
KUH-432
Chitti pulari
Herb
Whole plant
Increase milk production
Goat, cow, buffaloes
Powder
Flour
Oral
Continuously

Citrullus colocynthis (L.) Schrad. Cucurbitaceae
KUH-433
Karthuma
Herb
Root
Skin infection
Buffalo, cow, goat, sheep
Juice

Dermal
Two days

Convolvulus arvensis L. Convolvulaceae
KUH-434
Shankpuspi
Herb
Whole plant
Constipation
Cow, buffaloes, sheep
Crushed
Sugar
Oral
Four days

Coriandrum sativum L. Apiaceae
KUH-435
Dhania
Herb
Leaves, root
Antidiuretic
Buffaloes
Decoction
Water
Oral
One week

Curcuma longa L. Zingiberaceae
KUH-436
Haldi
Tree
Leaves
Wound healing
Cow
Decoction
Sugar
Oral
Two days

Cynodon dactylon L. Poaceae
KUH-437
Wakha
Herb
Whole plant
Wound healing, analgesic
Cow, buffaloes
Concoction
Milk
Oral
Two days

Cynoglossum lanceolatum Forssk. Boraginaceae
KUH-438
Pachy
Herb
Root
Common cold
Cow, buffaloes
Crushed
Water
Oral
Two days

Cyperus niveus Retz. Cyperaceae
KUH-439
Kulio
Herb
Whole plant
Common cold, stomach worms, joint pains
Cow, goat
Crushed
Water
Oral
Four to five days

Datura inoxia Mill. Solanaceae
KUH-440
Mangaz
Herb
Whole plant
Antilice
Sheep, cow
Paste
Milk
Dermal
Three days

Hedera nepalensis K. Koch Araliaceae
KUH-441
Zalai
Shrub
Leaves
To remove leeches
Sheep
Infusion

Nasal
Three days

Melia azedarach L. Meliaceae
KUH-442
Dhrek
Tree
Leaves
Stomach flatulence
Cow, buffaloes
Powder
Sugar
Oral
Four days

Mentha arvensis Linn. Lamiaceae
KUH-443
Pudina
Herb
Leaves
External parasite
Cow
Paste

Dermal
Four days

Morus alba L., Moraceae
KUH-444
Toot
Tree
Leaves, fruit
Laxative
Buffaloes
Crushed
Milk
Oral
Two days

Morus nigra L. Moraceae
KUH-445
Tor toot
Tree
Leaves
Tonic, laxative
Cow, buffaloes
Powder
Water
Oral
Three days

Nerium oleander L. Apocynaceae
KUH-446
Ghanderay
Shrub
Whole plant
Stomachache
Sheep
Concoction
Water
Oral
One week

Ocimum basilicum L. Lamiaceae
KUH-447
Kashmalay
Shrub
Leaves
Gastrointestinal
Buffaloes
Decoction
Water
Oral
Four days

Punica granatum L. Lythraceae
KUH-448
Anar
Shrub
Fruit, leaves
Anthelmintic
Cow, buffaloes, goat
Decoction
Milk
Oral
Two days

Ricinus communis Linn. Euphorbiaceae
KUH-449
Arund
Shrub
Leaves, stem
Common cold
Buffaloes, cow
Powder
Flour
Oral
Three days

Rumex hastatus D. Don. Polygonaceae
KUH-450
Tarooky
Shrub
Root and leaves
Wound healing
Goat, cow, buffaloes
Powder
Flour
Oral
Four days

Solanum surrattense Burm. f. Solanaceae
KUH-451
Kandiari
Herb
Whole plant
Fever, cough, intestinal infections
Cow, buffaloes, goat, sheep
Crushed
Flour
Oral
One week

Sonchus asper (L.) Hill. Asteraceae
KUH-452
Spingul
Herb
Whole plant
Milk production
Goat, cow, buffaloes
Decoction
Flour
Oral
Continuously

Tagetes minuta L. Asteraceae
KUH-453
Ban hanjari
Herb
Leaves
Skin infections
Cow, buffaloes
Juice

Dermal
Five days

Tamarix aphylla (L.) H. Karst. Tamaricaceae
KUH-454
Khagal
Tree
Leaves
Kill worms of wounds
Cow
Paste

Dermal
Four days

Tinospora cordifolia Miers. Menispermaceae
KUH-455
Giloe
Climber
Whole plant
Skin infections
Cow, goat
Poultice

Dermal
Three days

Tribulus terrestris Linn. Zygophyllaceae
KUH-456
Markondai
Herb
Whole plant
Chronic cough
Cow, buffaloes, goat
Crushed
Sugar
Oral
One week

Trifolium repens L. Papilionaceae
KUH-457
Shoutal
Shrub
Root
Tonic, laxative
Goat, cow
Powder
Flour
Oral
One day

Triticum aestivum L. Poaceae
KUH-458
Gandam
Herb
Seeds
Common cold, dysentery
Cow
Powder
Flour
Oral
Two days

Verbena officinalis L. Verbenaceae
KUH-459
Shamakay
Herb
Stem, leaves
Wound healing
Buffaloes
Decoction
Milk
Oral
Three days

Vitex negundo L. Verbenaceae
KUH-460
Marmandi
Shrub
Stem
Mange, fever, stomach problems
Cow, goat
Crushed, decoction
Sugar
Oral
Four to six days

Zizyphus nummularia W. & A. Rhamnaceae
KUH-461
Kurkunda
Tree
Leaves
Wound healing
Cow
Decoction
Sugar
Oral
Three days

Table 2: Habit and parts used of ethnoveterinary plants.

General attributes
Total plants
Percentage

Habit

    Herbs
22
55

    Shrubs
11
27

    Trees
06
15

    Climbers
01
02

Parts used

    Leaves
19
47

    Whole plant
13
32

    Root
07
17

    Stem
04
10

    Fruit
03
07

    Seed
01
02

Table 3: Informant consensus factor.

Disease categories
Nur
Nt
Fic

Gastrointestinal
260
13
0.95

Respiratory
22
02
0.95

Reproductive
47
03
0.95

Dermatological
12
04
0.77

Wounds
53
06
0.90

Antipyretic
14
07
0.53

Parasitic
45
05
0.90

General body tonic
09
03
0.75

Table 4: Fidelity level of highly utilized species.

Number
Plant species
Disease category
Ip
Iu
FL %

01
Asparagus gracilis
Reproductive
29
31
93

02
Rumex hastatus
Wound healing
32
35
91

03
Tinospora cordifolia
Dermatological
28
31
90

04
Aloe barbadensis
Gastrointestinal
23
27
85

05
Convolvulus arvensis
Gastrointestinal
24
29
82

06
Tribulus terrestris
Respiratory
17
21
80

07
Zizypus nummularia
Wound healing
18
23
78

08
Chenopodium album
Wound healing
17
25
68

09
Artemisia brevifolia
Reproductive
19
30
63

10
Cannabis sativa
Parasitic
17
29
58

Figure 2: Number of plants used to treat different domestic animals.

Figure 3: Traditional medicines preparation.

4. Discussion

Livestock keeping is one of the most important economic sources of rural community of study area. The farmers and nomadic people of the area not only depend on plants to get fodder for their animals but also use different medicinal plants to treat various animal diseases. The majority of the people interviewed using ethnoveterinary plants have got this knowledge from their forefathers while some have learned from the other people. The majority of the farmers and nomadic pastoralists were not very well off and heavily dependent on medicinal plants due to their unaffordable potential of using modern veterinary drugs for their animals treatment.

The present study revealed that people of the region use 41 medicinal plants for their livestock health care. Similar studies have also been documented in other parts of Pakistan [5, 15]. Traditional healers of the region mostly use herbs for the treatment of their animals that might be due to the fact that herbs are available everywhere and easy to collect as compared with other growth forms. The results indicate the abundance of herbs in the study area and their high usage might also be due to the strong efficacy of herbaceous plants against livestock ailments. The same findings were also reported from other studies conducted at different parts of the world [16, 17].

The wider utilization of this Asteraceae family might be due to its higher abundance in the study area or might be due to high bioactivity. Similar studies have also been reported from other parts of world [18] and from Pakistan [19, 20] where traditional healers mostly use the member of Asteraceae family for the preparation of traditional medicines for the treatment of different livestock and human ailments. This observation is however different from that of Appidi et al. [21] and Offiah et al. [22] who in an ethnoveterinary survey reported Fabaceae family as the highest. The difference among studies might be related to the different dominant vegetation of the areas or might be associated with traditional beliefs of different cultures in using specific plants traditionally.

Most of the ethnoveterinary recipes in the study region are prepared using leaves of plants. The highest use of leaves in large number of ethnomedicinal and ethnoveterinary studies has also been documented from different parts of the world [23, 24]. Preferred use of leaves might be associated with ease of collection as compared to other plant parts. Leaves are the main site of photosynthetic apparatus and are involved in a variety of physiological processes of plants and produce numerous secondary metabolites that could be a possible reason for their effectiveness and efficacy against different livestock diseases. Local people also use whole plants after leaves for herbal formulation that could be a very destructive type of harvesting for rare and slowly growing plants from conservation point of view. Harvesting of leaves does not pose any serious impact on the life cycle of plants and is considered a sustainable type of harvesting. The present results are in contradiction with other studies where roots are the most widely used plant part in ethnoveterinary practices [25, 26].

Cows and buffaloes were the most commonly treated animals followed by goats and sheep in the studied region. Similar results have also been conducted by van der Merwe et al. [27] and Benítez et al. [16]. There was almost no mention of treating dogs, cats, or donkeys. This is probably because rural people do not generally keep animals as pets and because nonproduction animals are perceived as being more resistant than humans to different kinds of ailments. Production animals are also more important because of their socioeconomic importance in the local inhabitant life. The majority of the plants in the region are used to treat different types of gastrointestinal problems of the livestock like diarrhea, expulsion of worms, constipation, and so forth. It has already been found that stomach infections are more common in lactating animals which might be due to poor quality of fodder and drinking water [17]. Informant consensus results also showed the highest informant citation for gastrointestinal, respiratory, and gynecological problems. The highest informant citation against these infections gives an indication of high prevalence of these diseases in the region. According to Heinrich et al. [11], high Fic values are very useful in the selection of specific plants for further search of bioactive compounds. Widely used medicinal plants for species ailments always score the highest fidelity level. The present study determined different plants like Asparagus gracilis, Rumex hastatus, Tinospora cordifolia, Aloe barbadensis, and so forth, scored highest fidelity values and should be further subjected to phytochemical and pharmacological investigation to prove their medicinal efficacy.

The method of drug preparation in many cases varied from individual to individual. The same plant material for the same ailment was prepared in different ways by different traditional veterinary healers. Traditional healers prepare ethnoveterinary recipes mostly in the form of powder and decoction in the study area. Deeba [28] powdering or boiling is the most common method of drugs extraction. These findings are in line with a study conducted in the Malakand valley of Pakistan [5] while they are contradictory with the studies conducted in other parts of the world [29, 30]. Most of the recipes are prepared using single plant mixture while some of the recipes are also prepared in the form of concoction and it is generally believed that potency of the drugs can be enhanced when used in concoction form [31]. These recipes are mostly taken orally in the study area due to the high prevalence of the internal diseases. These recipes are given to the livestock with their feed along with different types of ingredients like sugar, flour, milk, and so forth, in the region. Similar findings are also reported from other regions of the world [18, 32]. The use of these vehicles might be due to their enhancing potential of taste and medicinal properties of certain plant remedies. Uniformity was lacking regarding amount of medicines to be used among informants during the interview. It was determined that a contradiction in ethnoveterinary dosage is a serious drawback of traditional medicinal plants. Informants only provided the knowledge of observed time of recovery of animals in response to given recipes. Full recovery is confirmed when the animals restart proper feeding and activities. Similar findings are also reported by other ethnoveterinary studies conducted elsewhere [5, 22].

It was confirmed from the present study that men had better knowledge regarding ethnoveterinary practices as compared to women. The reason might be due to the fact that men are mostly favored in shift of knowledge while women in the majority of the cultures are considered for family’s care. This noticeable gender bias reflects the limited involvement of women in cattle production and herd health in Kohat region of Pakistan. Aged males had much more indigenous knowledge as compared to young generation which might be due to the lack of interest in such practices. Therefore, documentation of ethnoveterinary practices is an essential step toward the conservation of such knowledge before its extinction.

5. Conclusions

Local farmers and nomads of the region utilize different medicinal plants for the treatment of livestock due to their low income status and high expenses of western drugs. Traditional healers possess tremendous expertise in preparing herbal formulations of medicinal plants. Gastrointestinal infections were most common in the studied region; therefore attention should be given to provide good quality fodder and water to the livestock. Plants with high informant consensus and fidelity level should be subjected to further in vitro investigation for their phytochemical analysis and pharmacological activities. Young generation should be mobilized to take interest in ethnoveterinary practices in order to conserve this knowledge.

Questionnaire of Ethnoveterinary Data Collection

Informants’ Consent for the Participation in the Study

I__________ (name of informant) hereby give my full consent and conscious to participate in this study and declare that to the best of my knowledge the information that I have provided is true, accurate, and complete.

Date _____________ (signature/thumb impression of informant).

Informants’ Details

 Name Gender Age Occupation Education Location/residence

Data about Medicinal Plant and Its Use

 Number of plants known Names of plants (local names) Plant part used Name of disease(s) treated Type of animal treated Method of crude drug preparation Use of single or mixture of plants Ingredients used Recovery time Route of administration Dosage

Informant Consensus Factor and Fidelity Level

 Name of plants used against disease category 1 Name of plants used against disease category 2 Name of plants used against disease category 3 Name of plants used against disease category 4 Name of plants used against disease category 5 Name of plants used against disease category 6 Name of plants used against disease category 7 Name of plants used against disease category 8 Name of plants used against disease category 9 Name of plants used against disease category 10

Remarks

Plant identified as —— (botanical name and family)

Signature of researcher

Conflict of Interests

The authors declare that they have no competing interests.

Authors’ Contribution

Akash Tariq and Muhammad Adnan designed the research project. Akash Tariq and Sakina Mussarat conducted the field work. Akash Tariq, Sakina Mussarat, Abdul Latif Khan, Naser M. AbdElsalam, and Riaz Ullah analyzed the data and wrote the whole paper. Muhammad Adnan and Akash Tariq supervised all the stages of research work and provided comments on the draft. All the authors have read and approved the final paper.

Acknowledgments

The authors are thankful to the Deanship of Scientific Research, King Saud University, Riyadh, Saudi Arabia, for funding the work through the Research Group Project no. RGP-210. The authors are also very thankful to the local informants for sharing their valuable knowledge.

References

S. V. Veen, “Sense or nonsense? Traditional methods of animal disease prevention and control in African savannah,” in Ethnoveterinary Research and Development, C. M. McCorkle, E. Mathias, and T. W. Schillhorn van Veen, Eds., p. 338, Intermediate Technology Publications, London, UK, 1996.

C. Lans, N. Turner, T. Khan, G. Brauer, and W. Boepple, “Ethnoveterinary medicines used for ruminants in British Columbia, Canada,” Journal of Ethnobiology and Ethnomedicine, vol. 3, article 11, 2007.

S. Ganesan, M. Chandhirasekaran, and A. Selvaraj, “Ethnoveterinary healthcare practices in southern districts of Tamil Nadu,” Indian Journal of Traditional Knowledge, vol. 7, no. 2, pp. 347–354, 2008.

C. H. Rahman, A. Ghosh, and S. Mandal, “Studies on the Ethno veterinary medicinal plants used by the tribes of Birbhum district, West Bengal,” Indian Journal of Traditional Knowledge, vol. 33, pp. 333–338, 2009.

H. Ul Hassan, W. Murad, A. Tariq, and A. Ahmad, “Ethnoveterinary study of medicinal plants in Malakand Valley, District Dir (Lower), Khyber Pakhtunkhwa, Pakistan,” Irish Veterinary Journal, vol. 67, no. 1, article 6, 2014.

A. M. Abbasi, S. M. Khan, M. Ahmad, M. A. Khan, C. L. Quave, and A. Pieroni, “Botanical ethnoveterinary therapies in three districts of the Lesser Himalayas of Pakistan,” Journal of Ethnobiology and Ethnomedicine, vol. 9, article 84, 2013.

Z. Farooq, Z. Iqbal, S. Mushtaq, G. Muhammad, M. Z. Iqbal, and M. Arshad, “Ethnoveterinary practices for the treatment of parasitic diseases in livestock in Cholistan desert (Pakistan),” Journal of Ethnopharmacology, vol. 118, no. 2, pp. 213–219, 2008.

A. Hussain, M. N. Khan, Z. Iqbal, and M. S. Sajid, “An account of the botanical anthelmintics used in traditional veterinary practices in Sahiwal district of Punjab, Pakistan,” Journal of Ethnopharmacology, vol. 119, no. 1, pp. 185–190, 2008.

S. Shinwari, R. Qureshi, and E. Baydoun, “Ethnobotanical study of Kohat Pass (Pakistan),” Pakistan Journal of Botany, vol. 43, pp. 135–139, 2011.

M. Giday, Z. Asfaw, Z. Woldu, and T. Teklehaymanot, “Medicinal plant knowledge of the Bench ethnic group of Ethiopia: an ethnobotanical investigation,” Journal of Ethnobiology and Ethnomedicine, vol. 5, article 34, 2009.

M. Heinrich, A. Ankli, B. Frei, C. Weimann, and O. Sticher, “Medicinal plants in Mexico: healers' consensus and cultural importance,” Social Science and Medicine, vol. 47, no. 11, pp. 1859–1871, 1998.

M. Canales, T. Hernández, J. Caballero et al., “Informant consensus factor and antibacterial activity of the medicinal plants used by the people of San Rafael Coxcatlán, Puebla, México,” Journal of Ethnopharmacology, vol. 97, no. 3, pp. 429–439, 2005.

J. Friedman, Z. Yaniv, A. Dafni, and D. Palewitch, “A preliminary classification of the healing potential of medicinal plants, based on a rational analysis of an ethnopharmacological field survey among Bedouins in the Negev Desert, Israel,” Journal of Ethnopharmacology, vol. 16, no. 2-3, pp. 275–287, 1986.

R. T. Trotter and M. H. Logan, “Informants consensus: a new approach for identifying potentially effective medicinal plants,” in Plants in Indigenous Medicine and Diet, N. L. Etkin, Ed., pp. 91–112, Redgrave, Bedford Hill, NY, USA, 1986.

M. A. Khan, M. A. Khan, and M. Hussain, “Ethnoveterinary medicinal uses plants of Poonch valley Azad Kashmir,” Pakistan Journal of Weed Sciences Research, vol. 18, no. 4, pp. 495–507, 2012.

G. Benítez, M. R. González-Tejero, and J. Molero-Mesa, “Knowledge of ethnoveterinary medicine in the Province of Granada, Andalusia, Spain,” Journal of Ethnopharmacology, vol. 139, no. 2, pp. 429–439, 2012.

D. Luseba and D. van der Merwe, “Ethnoveterinary medicine practices among Tsonga speaking people of South Africa,” Onderstepoort Journal of Veterinary Research, vol. 73, no. 2, pp. 115–122, 2006.

H. Yineger, E. Kelbessa, T. Bekele, and E. Lulekal, “Ethnoveterinary medicinal plants at Bale Mountains National Park, Ethiopia,” Journal of Ethnopharmacology, vol. 112, no. 1, pp. 55–70, 2007.

I. Khan, N. M. AbdElsalam, H. Fouad, A. Tariq, R. Ullah, and M. Adnan, “Application of ethnobotanical indices on the use of traditional medicines against common diseases,” Evidence-Based Complementary and Alternative Medicine, vol. 2014, Article ID 635371, 21 pages, 2014.

N. Akhtar, A. Rashid, W. Murad, and E. Bergmeier, “Diversity and use of ethno-medicinal plants in the region of Swat, North Pakistan,” Journal of Ethnobiology and Ethnomedicine, vol. 9, no. 1, article 25, 2013.

J. R. Appidi, D. S. Grierson, and A. J. Afolayan, “Ethnobotanical study of plants used for the treatment of diarrhoea in the Eastern Cape, South Africa,” Pakistan Journal of Biological Sciences, vol. 11, no. 15, pp. 1961–1963, 2008.

N. V. Offiah, S. Makama, I. L. Elisha et al., “Ethnobotanical survey of medicinal plants used in the treatment of animal diarrhoea in Plateau State, Nigeria,” BMC Veterinary Research, vol. 7, article 36, 2011.

C. P. Kala, “Ethnomedicinal botany of the Apatani in the Eastern Himalayan region of India,” Journal of Ethnobiology and Ethnomedicine, vol. 1, article 11, 2005.

J. A. Bhat, M. Kumar, and R. W. Bussmann, “Ecological status and traditional knowledge of medicinal plants in Kedarnath Wildlife Sanctuary of Garhwal Himalaya, India,” Journal of Ethnobiology and Ethnomedicine, vol. 9, no. 1, article 1, 2013.

J. R. S. Tabuti, S. S. Dhillion, and K. A. Lye, “Ethnoveterinary medicines for cattle (Bos indicus) in Bulamogi county, Uganda: plant species and mode of use,” Journal of Ethnopharmacology, vol. 88, no. 2-3, pp. 279–286, 2003.

D. Hunde, Z. Asfaw, and E. Kelbessa, “Use and management of ethnoveterinary medicinal plants by indigenous people in “Boosat”, Welenchetti area,” Ethiopian Journal of Biological Sciences, vol. 3, pp. 113–132, 2004.

D. van der Merwe, G. E. Swan, and C. J. Botha, “Use of ethnoveterinary medicinal plants in cattle by Setswana-speaking people in the Madikwe area of the North West Province of South Africa,” Journal of the South African Veterinary Association, vol. 72, no. 4, pp. 189–196, 2001.

F. Deeba, Documentation of ethnoveterinary practices in urban and peri-urban areas of Faisalabad, Pakistan [Ph.D. thesis], University of Agriculture, Faisalabad, Pakistan, 2009.

A. P. Dold and M. L. Cocks, “Traditional veterinary medicine in the Alice district of the Eastern Cape Province, South Africa,” South African Journal of Science, vol. 97, no. 9-10, pp. 375–379, 2001.

E. Lulekal, E. Kelbessa, T. Bekele, and H. Yineger, “An ethnobotanical study of medicinal plants in Mana Angetu District, southeastern Ethiopia,” Journal of Ethnobiology and Ethnomedicine, vol. 4, article 10, 2008.

D. Abebe and A. Ayehu, Medicinal Plants and Enigmatic Health Practices of Northern Ethiopia, Berhanena Selam Printing Enterprise, Addis Ababa, Ethiopia, 1993.

A. Jabbar, M. A. Raza, Z. Iqbal, and M. N. Khan, “An inventory of the ethnobotanicals used as anthelmintics in the southern Punjab (Pakistan),” Journal of Ethnopharmacology, vol. 108, no. 1, pp. 152–154, 2006.