The Scientific World Journal

Volume 2014 (2014), Article ID 304120, 5 pages

http://dx.doi.org/10.1155/2014/304120

Research Article

Evaluation of the Nutritive and Organoleptic Values of Food Products Developed by Incorporated Catharanthus roseus (Sadabahar) Fresh Leaves Explore Their Hypoglycemic Potential

Gita Bisla,¹ Shailza Choudhary,¹ and Vijeta Chaudhary²

¹Department of Food Science and Nutrition, Banasthali University, Rajasthan 304022, India

²Department of Commerce, V.M.L.G College, Ghazibad, Uttar Pradesh, India

Received 4 August 2013; Accepted 1 September 2013; Published 24 March 2014

Academic Editors: A. Chicco and N. Vikram

Copyright © 2014 Gita Bisla et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Diabetes becomes a real problem of public health in developing countries, where its prevalence is increasing steadily. Diabetes mellitus can be found in almost every population in the world. Since the Ayurvedic practice started in India, plants are being used in the cure of diseases. Although the Catharanthus roseus have been used for their alleged health benefits and avail their hypoglycemic effect, used as medicine by diabetics. Medicinal plants have rarely been incorporated in food preparations. To fill these lacunae, food products were prepared by using Catharanthus roseus (Sadabahar) fresh leaves with hypoglycemic properties. Commonly consumed recipes in India are prepared for diabetic patients and were developed at different levels at 3 g, 4 g, and 6 g per serving. Food product development and their acceptability appraisal through organoleptic evaluation were carried out by semitrained panel comprising 15 trained panelists from the department of Food Science and Nutrition, Banasthali University. Seven products were developed by incorporating Catharanthus roseus fresh leaves. Nine point hedonic scale was used as a medium to know about the product acceptability at various variances. All products are moderately acceptable at different concentrations except product fare “6 g” which was more acceptable than the standard. Among the three variations of incorporating the Catharanthus roseus (Sadabahar) Leaves, 3 g variation is more acceptable than other variations.

1. Introduction

Diabetes mellitus is a chronic condition characterized by major derangements in glucose metabolism and abnormalities in fat and protein metabolism [1]. Patients use many products and modalities to treat diabetes or its comorbidities. People with diabetes are 1.6 times more likely to use a complementary and alternative medicine (CAM) treatment modality than those without diabetes [2].

The World Health Organization (WHO) estimates that currently more than 180 million people worldwide have diabetes and it is likely to double by 2030, with India, China, and the United States predicted to have the largest number of affected individuals [3]. Many plants and their active chemical compounds have demonstrated activity in the treatment of various disorders [4]. According to ethnobotanical information, more than 800 plants are used as traditional remedies in one or other form for the treatment of diabetes [5]. With the disturbing rise in the prevalence of this metabolic disease and associated healthcare cost, interest in alternative or complementary therapies has been grown. This interest is due to reasons such as ease of access, better culture acceptability and compatibility, cost effectiveness, and also the bid to “Go Natural”.

A plant is a living organism able to convert inorganic material into organic molecules necessary for the life of the plant itself and serves as food, for example, insects, animals, and humans. Plants also provide medicines and many other commodities. However, our knowledge about plants with their enormous diversity is still limited in many aspects. Still many novel products might be obtained from plants; however, this is hampered by the rapid loss of plant diversity on earth due to, for example, deforestation. The sustainable exploitation of plants for food and medicines requires extensive knowledge about plants. As the world’s population grows to an estimated 9 billion people in 2050, the availability of food and medicines for all people in the future should be a concern to all of us. Up to now, plants remain a primary source of medicines for most people in the world. Therefore, research in plant science is of great importance for human health, both for the production of healthier food and for development and production of medicines [6].

The important values of some plants have long been published but a large number of them remain unexplored. So, there is a necessity to explore their uses and to conduct pharmacognostic and pharmacological studies to ascertain their therapeutic properties. Despite considerable progress in the management of diabetes mellitus by synthetic drugs, the search for natural antidiabetic plant products for controlling diabetes is going on. There are many hypoglycemic plants known through the folklore but their introduction into the modern therapy system awaits the discovery of animal test system that is closely parallel to the pathological course of diabetes in human beings. Hypoglycemic activity has been reported in many plants during the last twenty years [7].

In India, herbal medicines date back several thousand years to the Rig-Veda, a collection of Hindu sacred verses, system of health care known as Ayurvedic medicine, which is still widely practiced in India today. Catharanthus roseus belongs to the family apocynaceae [8] Catharanthus roseus is commonly called as Periwinkle, Madagascar periwinkle, and Sadabahar. It grows throughout India and is found as an escape in waste places and sandy tracts. More than 130 different compounds have been reported including about 100 monoterpenoid indole alkaloids [9]. As an important medical plant, it has a good antioxidant potential throughout its parts under drought stress [10]. There are several health benefits of Catharanthus roseus leaves such as maintaining blood sugar [11], lowering high blood pressure [12], menstruation irregularities, Hodgkin’s disease [13], and as antioxidant [14].

Recently there has been a shift in universal trend from synthetic to herbal medicine, which we can say “Return to Nature.” Subsequently, with this background in mind, this study was embarking on the study of hypoglycemic effect of Catharanthus roseus fresh leaves in type II Diabetes Mellitus. Most of the people do not know Catharanthus roseus nutritional quality, health benefits, and how to use and what to use. They are not utilized to its full potential. So, the basic aim of this study is to create awareness about Catharanthus roseus leaves among the population. As many other herbal plants established in the indigenous system of medicine for their antidiabetic potentials and this plant has to be established in this indigenous system of medicines. Therefore, the present study was undertaken to develop commonly consumed food preparations for diabetics by incorporating Catharanthus roseus fresh leaves and evaluate their acceptability. Traditional foods reflect cultural inheritance and have left their imprints on contemporary dietary patterns [15].

2. Material and Methods

2.1. Sample Description

Catharanthus roseus fresh leaves were collected from the main campus of Banasthali University, Rajasthan, India. The leaves were washed thoroughly with tap water followed with sterilized distilled water for the removal of dust and sand particles. Commonly seven consumed recipes in India are prepared for diabetic patients were developed at different levels at 3 g, 4 g, and 6 g per serving.

2.2. Panel Selection and Training

Food product development and their acceptability appraisal through organoleptic evaluation carried out by a trained panel comprising 30 panelists from the department of Food Science and Nutrition, Banasthali University. They were preselected on the basis of good health conditions, time availability, no allergy plants products, any aversion to Catharanthus roseus, and willingness to participate. Panelists were then subjected to preliminary acuity tests to investigate their ability to recognize basic taste, basic aromas, and to describe basic attributes in prepared products by triangle method [16, 17]. After the screening process fifteen panelists were selected. Twelve panelists were female and three were male in the range of 20–35 years old of age. They went under training to perform the sensory evaluation of the products.

Training consisted of: (1) initial orientation session where panelists received detailed explanation about the organoleptic methodology; (2) group meetings for general description of the Catharanthus roseus fresh leaves incorporating food products development, which required 30 minutes to 1-hour sessions; (3) individual training during opening sessions panelists, took part in exercise. They were provided with the three samples (plain, salted, and sugary flavors in mango shake), which were diluted in 2% of cinnamon solution. They were asked to smell and taste them and list as many aromas and flavor terms as possible for each sample and discuss individual results to come up with a consensus.

2.3. Outcome Measures

The panelists performed organoleptic appraisal by 9 point hedonic test [18] to assess the overall products preferences. Evaluation of taste, flavor, color, texture, appearance, after taste, and overall acceptability were made in the scale. Two samples were during a 60-minute session and the evaluations were repeated two more times and they were asked to rinse their penchant well with water between samples. For this purpose, panelists used room-temperature drinking water.

2.4. Quality Control

(i)Panelist should be fit and free from tiredness to reduce the possibility of errors.(ii)Ensure that there is sufficient light at the judgment site.(iii)If too many people congregate, it may interfere with the sensory evaluation, site should be free from distraction.(iv)Drink water between the evaluations of samples.(v)The panelists were instructed not to swallow the samples.(vi)Proper weighing of the raw materials to be used in the cooking.(vii)Area should be odor free.(viii)There should be proper ventilation.(ix)Instruction to the panelists should be understandable, concise, and appropriate to the test.

2.5. Standardization of Food Products and Nutritive Value of Food Products

Fresh leaves of medicinal plants, namely, Catharanthus roseus, leaves incorporating in food products, respectively, developed different levels at 3 g, 4 g, and 6 g per serving. Food preparations made by using different cooking methods (Table 1). Nutrient content per serving of the products incorporated Catharanthus roseus fresh leaves was calculated from the values given in nutritive value of Indian foods [19].

Table 1: Food preparations prepared with different methods of cooking by incorporating Catharanthus roseus fresh leaves.

Food preparations
Method of processing

Fure
Steaming

Rasam
Roasting and pressure cooking

Stuffed idli
Fermentation and steaming

Palak methi muthia
Steaming

Methi paratha
Stir frying

Coriander chutney
Grinding

Cucumber soup
Pressure cooking

2.6. Statistical Analysis

The data were subjected to statistical analysis using Statistical Package for Social Sciences (SPSS) version 16.0. Mean ± SD was used to obtain the differences in organoleptic scores, within different levels of incorporation of Catharanthus roseus fresh leaves in food preparations. Level of significance was accepted at .

3. Results

3.1. Organoleptic Evaluation

All the food products incorporated with Catharanthus roseus (Sadabahar) fresh leaves were found to be organoleptically satisfactory at different variations. Comparing the food products at different variations with standard of the food products, respectively. Although the scores of standard was more acceptable than all the variations made at different concentrations. It was also observed that the concentration of Catharanthus roseus fresh leaves in the samples was inversely proportional to the acceptability scores except in product fure where the scores of “6 g” were better than all the variations made at different concentrations for all attributes. As the concentration was increased, the mean scores for appearance, Color, flavor, texture, taste, after taste, and overall acceptability were found to be increased.

In Catharanthus roseus (Sadabahar) fresh leaves incorporated food products (Table 2), namely, fure, the most acceptable level of incorporation was 6 g and the mean scores for overall acceptability were . However, scores for all organoleptic characteristics of fure within 3 g and 4 g levels of incorporation were found to be nonsignificantly different.

Table 2: Organoleptic evaluation of food products developed by incorporation of Catharanthus roseus fresh leaves.

Food products
g/serving
Appearance
Color
Texture
After taste
Overall acceptability

Fure
Standard

3 g

4 g

6 g

Rasam
Standard

3 g

4 g

6 g

Stuffed idli
Standard

3 g

4 g

6 g

Palak methi muthia
Standard

3 g

4 g

6 g

Methi parantha
Standard

3 g

4 g

6 g

Coriander chutney
Standard

3 g

4 g

6 g

Cucumber soup
Standard

3 g

4 g

6 g

All values are found to be Nonsignificant.

Rasam and Stuffed Idli were moderately acceptable and the scores for their overall acceptability awarded by the panel of judges were at 3 g and at 4 g, respectively. Nonsignificant difference was noticed in scores for all organoleptic characteristics of Rasam and Stuffed Idli within variations of incorporation of Catharanthus roseus (Sadabahar) fresh leaves (Table 2). However, the acceptable level of incorporation varied in different food products. It has been found that when the level of incorporation of fresh leaves increased beyond the accepted levels in any food products,the mean scores for all organoleptic characteristics decreased.

For palak methi muthia, methi parantha, coriander chutney, and cucumber soup the most acceptable level of incorporation of Catharanthus roseus (Sadabahar) fresh leaves was 3 g per serving and the respective scores for overall acceptability ranged from (palak methi muthia) to (cucumber soup). Nonsignificant differences were found in scores for all organoleptic characteristics of food products (Table 2).

3.2. Nutritive Evaluation

Energy content of all the food products prepared by incorporating 3 g Catharanthus roseus (Sadabahar) fresh leaves per serving was varied from 13.31 Kcal of cucumber soup to 240.34 Kcal per serving of methi parantha (Table 3). Protein content was the highest in fure (12.51 g/serving) and the lowest in cucumber soup (0.34 g/serving). Fat content ranged between 0.03 g in cucumber soup and 12.51 g per serving in fure. Carbohydrate content of 3 g Catharanthus roseus (Sadabahar) fresh incorporated food products varied from 1.18 g of soup to 35.66 g per serving of stuffed idli. The highest fibre content was found in coriander chutney (0.75 g/serving) and minimum in soup was (0.05 g/serving). The hypoglycemic activity of Catharanthus roseus leaves may be due to the presence of alkaloid-like vincristine and vinblastine through stimulation of cell activity leading to increased insulin production and release [20].

Table 3: Nutritive evaluation of food products developed by incorporation of 3 g Catharanthus roseus fresh leaves per serving.

Food products
Cooked weight/serving (g)
Energy (kcal)
Protein (g)
Fat (g)
Carbohydrate (g)
Fiber (g)

Fure
80
137.34
12.51
3.87
34.58
0.16

Rasam
244
80.2
3.43
3.58
17.1
0.69

Stuffed idli
78
142.76
7.07
0.84
35.66
0.58

Palak methi muthia
65
168.52
10.58
2.65
23.04
0.12

Methi paratha
92
240.34
11.32
7.98
29.65
0.11

Coriander chutney
46
55
1.67
0.07
3.5
0.75

Cucumber soup
185
13.31
0.34
0.03
1.18
0.05

4. Conclusions

The scores of standard were more acceptable than all the variations made at different concentrations. It was also observed that the concentration of Catharanthus roseus fresh leaves, in the samples was inversely proportional to the acceptability scores. Three g per serving Catharanthus roseus freshleaves incorporation in food products developed was found to be organoleptically acceptable by the semitrained panel members. Amongst the incorporated food preparations, the mean scores for overall acceptability were highest for fure and lowest for methi parantha. Thus, this holds great promise for future research for the formulation of potent antidiabetic drug for the present plant.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

References

NOIC, Diabetes Among African Americans, National Diabetes Information Clearinghouse.

L. E. Egede, X. Ye, D. Zheng, and M. D. Silverstein, “The prevalence and pattern of complementary and alternative medicine use in individuals with diabetes,” Diabetes Care, vol. 25, no. 2, pp. 324–329, 2002.

S. Wild, G. Roglic, A. Green, R. Sicree, and H. King, “Global prevalence of diabetes: estimates for the year 2000 and projections for 2030,” Diabetes Care, vol. 27, no. 5, pp. 1047–1053, 2004.

E. A. Palombo, “Phytochemicals from traditional medicinal plants used in the treatment of diarrhoea: modes of action and effects on intestinal function,” Phytotherapy Research, vol. 20, no. 9, pp. 717–724, 2006.

F. J. Alarcon-Aguilara, R. Roman-Ramos, S. Perez-Gutierrez, A. Aguilar-Contreras, C. C. Contreras-Weber, and J. L. Flores-Saenz, “Study of the anti-hyperglycemic effect of plants used as antidiabetics,” Journal of Ethnopharmacology, vol. 61, no. 2, pp. 101–110, 1998.

G. A. Cordell, “Natural products in drug discovery—creating a new vision,” Phytochemistry Reviews, vol. 1, no. 3, pp. 261–273, 2002.

Z. Daniel and H. Maria, Domestication of Plants in the Old World, University Press, Oxford, UK, 3rd edition, 2000.

C. A. Jaleel, “change in non enzymatic antioxidants and ajmalicine production in Catharanthus roseus with different soil salinity regimes,” Botany Research International, vol. 2, no. 1, pp. 1–6, 2009.

D. M. Pereira, J. Faria, P. Valentao, M. Sottomayor, and P. B. Andrade, “Exploiting Catharanthus roseus roots: source of antioxidants,” Journal of Food Chemistry, vol. 6, pp. 235–242, 2010.

N. Rasool, K. Rizwan, M. Zubair, K. U. R. Naveed, I. Imran, and V. U. Ahmed, “Antioxidant potential of different extracts and fractions of Catharanthus roseus shoots,” International Journal of Phytomedicine, vol. 3, no. 1, pp. 108–114, 2011.

S. Nammi, M. K. Boini, S. D. Lodagala, and R. B. S. Behara, “The juice of fresh leaves of Catharanthus roseus Linn. reduces blood glucose in normal and alloxan diabetic rabbits.,” BMC Complementary and Alternative Medicine, vol. 3, no. 1, p. 4, 2003.

N. Ara, M. Rashid, and M. D. S. Amran, “Comparison of hypotensive and hypolipidemic effects of Catharanthus roseus leaves extract with atenolol on adrenaline induced hypertensive rats,” Pakistan Journal of Pharmaceutical Sciences, vol. 22, no. 3, pp. 267–271, 2009.

P. R. H. Moreno, R. van der Heijden, and R. Verpoorte, “Effect of terpenoid precursor feeding and elicitation on formation of indole alkaloids in cell suspension cultures of Catharanthus roseus,” Plant Cell Reports, vol. 12, no. 12, pp. 702–705, 1993.

C. A. Jaleel, R. Gopi, P. Manivannan, M. Gomathinayagam, R. Sridharan, and R. Panneerselvam, “Antioxidant potential and indole alkaloid profile variations with water deficits along different parts of two varieties of Catharanthus roseus,” Colloids and Surfaces B, vol. 62, no. 2, pp. 312–318, 2008.

A. Trichopoulou, S. Soukara, and E. Vasilopoulou, “Traditional foods: a science and society perspective,” Trends in Food Science and Technology, vol. 18, no. 8, pp. 420–427, 2007.

A. J. Byer and D. A. Abram, “comparsion of triangular and two sample taste test method,” Food Technology, vol. 7, article 185, 1953.

M. A. Amerine, R. M. Pangborn, and E. B. Roessler, Principles of Sensory Evaluation of Food, Academic Press, New York, NY, USA, 1965.

D. Peryam and N. F. Girardot, “Advanced taste test,” Food Engineering, vol. 24, pp. 58–61, 1952.

C. Gopalan, B. V. Ramasastri, S. C. Balasubramanian, B. S. Narasinga Rao, Y. G. Deosthale, and K. C. Pant, Nutritive Value of Indian Foods, National Institute of Nutrition (ICMR), Hyderabad, India, 2004.

S. Ghosh and S. A. Suryawanshi, “Effect of Vinca rosea extracts in treatment of alloxan diabetes in male albino rats,” Indian Journal of Experimental Biology, vol. 39, no. 8, pp. 748–759, 2001.