The Scientific World Journal

Volume 2014 (2014), Article ID 378651, 10 pages

http://dx.doi.org/10.1155/2014/378651

Research Article

Intrastrain Comparison of the Chemical Composition and Antioxidant Activity of an Edible Mushroom, Pleurotus giganteus, and Its Potent Neuritogenic Properties

Chia-Wei Phan,1,2 Pamela David,1,3 Yee-Shin Tan,1,2 Murali Naidu,1,3 Kah-Hui Wong,1,3 Umah Rani Kuppusamy,1,4 and Vikineswary Sabaratnam1,2

¹Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

²Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

³Department of Anatomy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia

⁴Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia

Received 15 May 2014; Accepted 24 June 2014; Published 10 July 2014

Academic Editor: Paula B. Andrade

Copyright © 2014 Chia-Wei Phan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Two strains of Pleurotus giganteus (commercial and wild) were tested for their ability to induce neurite outgrowth in rat pheochromocytoma (PC12) and mouse neuroblastoma-2a (N2a) cells. Treatment with the mushroom extracts resulted in neuronal differentiation and neuronal elongation, but not nerve growth factor (NGF) production. Linoleic acid (4.5–5.0%, w/w) which is a major fatty acid present in the ethanol extract promoted NGF biosynthesis when augmented with low concentration of NGF (5 ng/mL). The two strains of mushroom were found to be high in protein (154–192 g kg⁻¹), total polysaccharides, phenolics, and flavonoids as well as vitamins B1, B2, and B3. The total phenolics present in the mushroom extracts were positively correlated to the antioxidant activity (free radical scavenging, ferric reducing power, and lipid peroxidation inhibition). To conclude, P. giganteus could potentially be used in well-balanced diet and as a source of dietary antioxidant to promote neuronal health.

1. Introduction

Neurite outgrowth is a critical process in neuronal formation and development. Malfunction of this event will lead to demolition of synaptic connections and extended series of neuronal dysfunctions like Alzheimer’s disease (AD) [1]. As AD progresses, programmed apoptotic neuronal death is triggered as a result of nerve growth factors (NGFs) depletion and oxidative stress exerted by reactive oxygen species (ROS) [2]. Therefore, strategies aimed at preserving and restoring the neurite network might be beneficial in treating AD. Dietary intake of antioxidants is also important to improve the intrinsic antioxidant mechanisms (superoxide dismutase, catalase, and glutathione peroxide) to avoid an environment where prooxidant species overwhelm antioxidant species.

Functional food plays a significant role in preventing or reducing severity of lifestyle diseases and improving physical and mental well-being of consumers. Edible and medicinal mushrooms are gaining recognition as preventative agents for age-related diseases including neurodegenerative diseases such as AD, Parkinson’s disease, and dementia [3]. At the current stage, medications are prescribed for mild, moderate, to severe Alzheimer’s disease to help delay or prevent behavioral symptoms, but that, too, is only for a limited time. Trials with NGF for Alzheimer’s disease had gained some degree of success, but the high molecular weight of the NGF protein seems to suggest that it could not cross the blood-brain barrier [4]. Considering the limitation of the existing preventive methods, intervention strategy using mushrooms as functional food is of utmost importance. Edible and medicinal mushrooms are packed with a wide array of bioactive and nutritional components that could scavenge reactive oxygen species (ROS) and exert neuroprotective effects and promote neuritogenesis and neuroregeneration.

There are ample studies available in the literature regarding the chemical composition of different mushroom species from all over the world. However, such information on Pleurotus giganteus (Berk.) Karunarathna & K.D. Hyde, a popular edible mushroom in China, Thailand, and Sri Lanka is scanty. In Malaysia, the mushroom is also consumed as a delicacy by the indigenous communities. Studies on P. giganteus include domestication and cultivation [5], liver protection [6], and anti-Candida activities [7]. It has been shown that extracts of this mushroom exhibited neurite outgrowth activity in rat pheochromocytoma (PC12) and mouse neuroblastoma-2a (N2a) cells [8, 9]. In this study, the neuritogenic effects of the commercial strain of P. giganteus were compared to that of the wild domesticated strain. The nutritional components including vitamins, fatty acids, and amino acids as well as the phenolic, flavonoid contents, and antioxidant activities were also evaluated and compared.

2. Materials and Methods

2.1. Mushroom Species

Fresh basidiocarps of P. giganteus (commercial strain KLU-M 1227) were collected from Nas Agro Farm and Dong Foong Biotech. Wild P. giganteus (KLU-M 1228) was collected from Ayer Hitam Forest Reserve, Puchong, Malaysia. The mushroom identity (molecular fingerprinting) was authenticated by Dr. Yee-Shin Tan from Mushroom Research Centre, University of Malaya. Voucher specimens were deposited in the Herbarium of University of Malaya. Domestication of the wild strain was carried out as previously reported [8]. The substrate formulation for basidiocarp formation of both commercial and domesticated strains was similar and consisted of rubber wood sawdust, rice bran, and calcium carbonate.

2.2. Chemicals

Gallic acid, rutin, 2,2-diphenyl-1-picrylhydrazyl (DPPH), L-ascorbic acid, ABTS [2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt], phosphate buffered saline (PBS), nerve growth factor (NGF), and dimethyl sulfoxide (DMSO) were purchased from Sigma (St. Louis, MO, USA). All other chemicals and solvents were of analytical grade and purchased from Merck (Darmstadt, Germany).

2.3. Proximate Analysis of Basidiocarps

The freeze-dried powder of basidiocarps of P. giganteus was analyzed for the nutritional components using the standard American Oil Chemists’ Society (AOCS) procedures [10]. Total fat, dietary fiber, and protein content were analyzed using AOAC 989.05, AOAC 985.25, and Kjeldahl method with boric acid modification, respectively. Carbohydrates were calculated using the formula carbohydrates (g) = 100 − (protein + fat + ash). Energy was calculated using the formula energy (kcal) = 4 × (protein + carbohydrate) + 9 × (fat) [10].

2.4. Determination of Free Sugars, Minerals, Vitamins, Fatty Acids, and Amino Acids

Free sugars and amino acids were analyzed by high performance liquid chromatography (HPLC). Minerals were determined using inductively coupled plasma optical emission spectrometry ICP-OES following the AOAC 985.01 and 922.02, as well as American Association of Cereal Chemists (AACC 40–70) procedures. Vitamin C was determined by the AOAC 967.21 method. Preparation of methyl esters of long-chain fatty acids was carried out based on AOCS Ce-2-66 test for further analysis by gas chromatography (GC). Omega-3 and Omega-6 fatty acids were analyzed using AOCS 1d-91 methods by capillary gas-liquid chromatography (GLC).

2.5. Preparation of Mushroom Extracts

Crude aqueous and ethanol extracts were prepared as previously described [8]. For aqueous extract, the freeze-dried basidiocarps powder was soaked in distilled water (1 : 20, w/v) for 24 h at room temperature and 200 rpm in a shaker. After double-boiling in a water bath at 100°C, the mixture was filtered (Whatman No. 4). The filtrate was then freeze-dried and kept at −20°C prior to use. To obtain crude ethanol extract, the freeze-dried powder was soaked in 95% (v/v) ethanol at room temperature for three days. The solvent was then evaporated using a rotary evaporator (Eyela N-1000, USA) and a brownish viscous extract was obtained.

2.6. Determination of Total Polysaccharides

The total polysaccharide content of the aqueous and ethanol extracts was determined using the phenol-sulphuric acid method with D-glucose as a reference [11]. Briefly, 1 mL of 5% phenol was added to 1 mL of sample solution, followed by 5 mL of concentrated H₂SO₄. The absorbance was measured after 10 min at 497 nm.

2.7. Determination of Total Phenolic Contents (TPC) in Mushroom Extracts

The total phenolic contents in the mushroom extracts, expressed as gallic acid equivalents (GAEs), were determined by the Folin-Ciocalteu method [12]. Fifty microliters of sample was mixed with an equal volume of Folin-Ciocalteu phenol reagent. After 3 min, 100 μL of Na₂CO₃ (10%) was added to the mixture. The reaction was kept in the dark for 90 min, after which the absorbance was read at 750 nm using a spectrophotometer. A calibration curve was prepared with different concentrations of gallic acid (0–100 μg/mL) as standard. TPC was expressed as mg GAE/g of extract.

2.8. Determination of Total Flavonoids in Mushroom Extracts

Total flavonoids in the mushroom extract were estimated by using the aluminum calorimetric method as previously described [13]. Mushroom extract of 150 μL was mixed with an equal volume of AlCl₃ (2%). After 10 min, the absorbance of the supernatant was measured at 435 nm by using an ELISA microplate reader (Sunrise, Austria). The total flavonoid content of the mushroom extract was expressed as rutin equivalents in microgram per gram extract (mg RE/g extract).

2.9. Evaluation of Antioxidant Activity of Mushroom Extracts

2.9.1. DPPH Scavenging Activity Assay

The DPPH free radical scavenging activity was determined as previously described [14]. Mushroom extracts of various concentrations (5 μL) were mixed with 195 μL of DPPH reagent. The reduction of the DPPH radical was determined by measuring the absorption at 517 nm using a microtiterplate reader (Tecan, Austria). The radical scavenging activity was calculated using the following equation: % radical scavenging activity = [(AbsBlank   −  AbsSample)/AbsBlank  × 100%], where AbsSample is the absorbance of the sample whereas AbsBlank is the absorbance of the DPPH solution. The antioxidant property of the extracts was expressed in terms of IC₅₀ value, that is, the concentration to quench 50% of available DPPH content. L-ascorbic acid (0–25 μM) was used as standard and butylated hydroxytoluene (BHT) was used as control.

2.9.2. FRAP (Ferric Reducing Antioxidant Power) Assay

The reducing power of mushrooms extracts was determined by the ferric reducing antioxidant potential (FRAP) assay as previously described [15]. To prepare FRAP reagent, 2.5 mL of FeCl₃·6H₂O solution (20 mM in 40 mM HCl) was mixed with 2.5 mL of 2,4,6-tri(2-pyridyl)-1,3,5-triazine (TPTZ) (10 mmol/L in 300 mmol/L acetate buffer). Then, 10 μL of mushroom extract was added to 300 μL of the FRAP reagent after which the absorbance of the product of the reaction between Fe²⁺ and TPTZ was measured at 593 nm against a blank for each sample. Ferrous sulfate (FeSO₄) of concentrations 0–20 mM was used as standard and BHT as control. The FRAP value was expressed as μM of FeSO₄ equivalents/g mushroom.

2.9.3. Inhibition of Lipid Peroxidation

The assay was based on the thiobarbituric acid reaction method [16]. Mushroom extracts of different concentrations (0–20 mg/mL) were mixed with 0.5 mL of egg yolk suspension and 0.5 mL of FeSO₄. The mixture was incubated at 37°C for an hour after which 0.5 mL of 20% trichloroacetic acid (TCA) and 1 mL of 0.8% thiobarbituric acid (TBA) were added. The mixture was then heated in boiling water for 15 min and centrifuged at 3500 rpm for 20 min. The absorbance of thiobarbituric acid reactive substances (TBARS) present in the supernatant was measured at 532 nm using a microtiterplate reader (Tecan, Austria). Result was expressed as percentage inhibition of lipid peroxidation at extract concentration of 10 mg/mL. BHT was used as a control in this assay.

2.10. Effect of Mushroom Extracts on Neuritogenesis

2.10.1. Cell Culture

Mouse neuroblastoma (N2a, CCL-131) and rat pheochromocytoma cells (adherent variant, PC-12Adh) were purchased from American Type Culture Collection (ATCC; MD, USA). N2a cells were cultured in Eagle’s minimum essential medium (MEM) with L-glutamine (PAA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (PAA), 100 U/mL penicillin, and 100 μg/mL streptomycin. PC12 cells were maintained in F-12K medium (Sigma) supplemented with 2.5% (v/v) heat-inactivated fetal bovine serum (PAA) and 15% (v/v) horse serum (PAA). All plates were incubated at 37°C in a humidified environment of 5% CO₂ and 95% air. The cells were routinely passaged every 2-3 days.

2.10.2. Neurite Outgrowth Assay

N2a and PC12 cells were seeded at a density of 5 × 10³ cells in growth medium per well in 24-well culture plates and incubated overnight. Mushroom extracts (0–50 μg/mL) were added to the cells and further incubated for 3 days. For N2a cells, the cells were induced to differentiate by replacing the growth medium with 5% serum medium. Nerve growth factor (50 ng/mL) was used as a positive control. After 3 days, the cells were then examined using an inverted light microscope (Nikon Eclipse TS100). Five random fields (200–300 cells/well) were examined in each well. The number of axon-like processes, defined as extensions longer than twice the cell body diameter, was recorded. The mean number of neurite-bearing cells was quantified by scoring the total number of neurite-bearing cells over the total number of viable cells per field. At least three independent experiments were conducted.

2.10.3. Measurement of NGF

The NGF level in the PC12 culture medium was performed following the ChemiKine NGF sandwich enzyme-linked immunosorbent assay (ELISA) kit procedure (Merck Millipore, Germany). After treatment, the culture medium was added into a microplate precoated with anti-mouse NGF polyclonal antibody. Anti-mouse NGF monoclonal antibody was then added. After 2 h, horseradish peroxidase- (HRP-) conjugated donkey anti-mouse IgG polyclonal antibody was added to react with TMB substrate solution. The color intensity of the sample was measured at 450 nm. The level of NGF was determined from a standard curve plotted with known concentrations of NGF.

2.11. Statistical Analysis

Results were expressed as mean ± standard deviation (SD) (). Analysis of variance (ANOVA) followed by Duncan’s test was performed to test for differences between means by employing Statgraphics Plus (Statistical Graphics Corp., Herndon, VA). Correlations between total polysaccharides, TPC, and antioxidant activities were determined by Pearson’s correlation coefficient () with the statistical program SPSS ver. 17.0 (SPSS Inc., Chicago, IL, USA). The statistical significance of mean differences was based on value of <0.05.

3. Results

3.1. Proximate Analysis of Mushroom Basidiocarps

The proximate nutritional components of commercial P. giganteus (KLU-M 1227) and domesticated wild P. giganteus (KLU-M 1228) are shown in Table 1. The commercial strain presented a significantly higher () carbohydrate, dietary fiber, total fat, and monosaturated fat content compared to the domesticated wild strain by 3.7, 2.7, 16, and 2.1%, respectively. Both strains showed no difference in the gross energy value and saturated fat contents. However, the wild strain presented a significantly higher () crude protein and polyunsaturated fat content.

Table 1: Chemical compositions, sugars, macro- and microelements, and vitamins of commercial Pleurotus giganteus (KLU-M 1227) and wild P. giganteus (KLU-M 1228).

Parameter
Pleurotus giganteus (commercial strain)
Pleurotus giganteus (wild strain)

Carbohydrate (g kg⁻¹)
672 ± 0.0a
647 ± 0.0b

Protein (g kg⁻¹)
154 ± 0.0c
192 ± 0.0d

Energy (kcal kg⁻¹)
3640 ± 0.00e
3640 ± 0.0e

Dietary fiber (g kg⁻¹)
333.5 ± 0.07f
324.5 ± 0.07g

Total fat (g kg⁻¹)
37.0 ± 0.0h
31.0 ± 0.0i

Saturated fat
9.7 ± 0.0j
9.5 ± 0.0j

Monosaturated fat
19.7 ± 0.0k
13.2 ± 0.0l

Polyunsaturated fat
7.8 ± 0.0m
8.3 ± 0.0n

Trans fat
ND
ND

Cholesterol (g kg⁻¹)
ND
ND

Free sugars (g kg⁻¹)

    Fructose
7.2 ± 0.2a
10.6 ± 0.4b

    Glucose
31.4 ± 0.7c
39.7 ± 0.2d

    Sucrose
ND
ND

    Lactose
ND
ND

    Maltose
ND
ND

    Maltotriose
ND
ND

Macroelements (g kg⁻¹)

    Potassium (as K)
13.46 ± 0.0a
11.71 ± 0.32b

    Phosphorus (as P)
5.27 ± 0.39c
4.01 ± 0.04d

    Magnesium (as Mg)
0.67 ± 0.00e
0.65 ± 0.00f

    Calcium (as Ca)
0.058 ± 0.00g
0.087 ± 0.01h

    Sodium (as Na)
0.058 ± 0.07g
0.047 ± 0.00i

Microelements (g kg⁻¹)

    Iron (as Fe)
0.019 ± 0.04a
0.014 ± 0.00b

    Zinc (as Zn)
0.027 ± 0.01c
0.042 ± 0.00d

    Manganese (as Mn)
0.041 ± 0.01e
0.043 ± 0.00e

    Copper (as Cu) (mg kg⁻¹)
0.60 ± 0.01f
0.228 ± 0.00f

    Selenium (as Se) (mg kg⁻¹)
ND
ND

Vitamins (g kg⁻¹)

    Vitamin B1
0.004 ± 0.01a
0.002 ± 0.00b

    Vitamin B2
0.009 ± 0.00c
0.009 ± 0.00c

    Vitamin B3
0.09 ± 0.10d
0.06 ± 0.02e

    Vitamin C
0.003 ± 0.00a
0.001 ± 0.01f

Each value is expressed as mean ± SD (). In each row, the different letters represent significant differences between samples (). ND: not detectable.

3.2. Determination of Sugars, Minerals, and Vitamins

The sugar composition of P. giganteus basidiocarps is given in Table 1. Glucose and fructose were detected in the basidiocarps of both strains. The glucose and fructose content of the domesticated wild strain was approximately 47% and 26% higher () than that of the commercial strain. For macroelements composition, potassium in the basidiocarps of commercial strain ( g kg⁻¹) was significantly higher () than the domesticated wild strain ( g kg⁻¹). On the other hand, the calcium level in the domesticated wild strain ( g kg⁻¹) was higher than that of the commercial strain ( g kg⁻¹). The vitamin profiles showed similarity, but the concentrations in the two strains were different. Vitamin C (ascorbic acid) in the wild strain was almost 3.9-fold higher than that of the commercial strain (Table 1). Vitamin B3 (niacin) is the most abundant vitamin found in this mushroom with and  g kg⁻¹ in the commercial and domesticated wild strain, respectively.

3.3. Determination of Amino Acids and Fatty Acids

All the essential amino acids (threonine, valine, methionine, isoleucine, leucine, phenylalanine, tryptophan, lysine, histidine, and arginine) were detected in this mushroom with the exception of tryptophan (Table 2). Among the essential amino acids, leucine (commercial strain  g kg⁻¹; domesticated wild strain  g kg⁻¹) was found to be the largest constituent, followed by phenylalanine and histidine. The methionine, isoleucine, and valine were present in small amounts ranging from 0.31 to 0.80% for both the P. giganteus strains. In particular, P. giganteus had the highest concentration of glycine, which is categorized as the nonessential amino acid. Also, the wild strains exhibited higher amount of glutamine, alanine, and asparagine as compared to the commercial strains.

Table 2: Amino acid content (g kg−1 protein) of Pleurotus giganteus KLU-M 1227and KLU-M 1228.

Amino acids
Pleurotus giganteus (commercial strain)
Pleurotus giganteus (wild strain)

Asp
6.9 ± 0.0c
7.0 ± 0.0c

Glu
15.7 ± 0.0jk
17.2 ± 0.1l

Ser
11.1 ± 0.01e
11.0 ± 0.01e

His*
15.4 ± 0.04ji
15.1 ± 0.05j

Gly
29.0 ± 0.11p
29.5 ± 0.04p

Thr*
5.3 ± 0.01b
5.2 ± 0.00b

Arg*
11.4 ± 0.04ef
12.1 ± 0.00fg

Ala
11.1 ± 0.01e
13.5 ± 0.00h

Tyr
5.8 ± 0.06b
5.9 ± 0.00b

Cys
ND
ND

Val*
8.0 ± 0.01d
7.1 ± 0.00c

Met*
3.1 ± 0.01a
3.1 ± 0.00a

Phe*
16.4 ± 0.09kl
16.5 ± 0.00kl

Ile*
7.7 ± 0.02cd
7.3 ± 0.00cd

Leu*
20.4 ± 0.10n
19.4 ± 0.00m

Lys*
14.5 ± 0.01i
12.8 ± 0.00gh

Asn
15.2 ± 0.07ij
24.6 ± 0.00o

Gln
ND
ND

Nva
ND
ND

Trp*
ND
ND

Asp: aspartate; Glu: glutamate; Ser: serine; His: histidine; Gly: glycine; Thr: threonine; Arg: arginine; Ala: alanine; Tyr: tyrosine; Cys: cystine; Val: valine; Met: methionine; Phe: phenylalanine; Ile: isoleucine; Leu: leucine; Lys: lysine; Asn: asparagine; Gln: glutamine; Nva: norvaline; Trp: tryptophan. Each value is expressed as mean ± SD (). In each row, the different letters represent significant differences between samples (). ND: not detected. *Essential amino acids.

Table 3 presents the fatty acid profile of basidiocarps of P. giganteus. Among the saturated fatty acids, palmitic acid (C16:0) was predominant with and  g kg⁻¹ in the commercial strain and wild strain, respectively. Amongst the polyunsaturated fatty acids, oleic acid was the predominant fatty acid in this species. Oleic acid (C18: 1n9c) in the commercial strain ( g kg⁻¹) was significantly () higher than that in the wild strain ( g kg⁻¹). Linoleic acid was also present in significant quantities (4.5–5.0 g kg⁻¹) in P. giganteus. The medium chain fatty acid, caprylic acid (C8:0), and the long chain fatty acid, stearic acid (C18:0), were also present. Capric, undecanoic, palmitoleic, and eicosadienoic acids were detected in trace amounts in P. giganteus.

Table 3: Fatty acids (g kg−1 dry weight) detected in the basidiocarps of Pleurotus giganteus.

Fatty acid
Pleurotus giganteus (commercial strain)
Pleurotus giganteus (wild strain)

C8:0
Caprylic
1.3 ± 0.0a
1.1 ± 0.0ab

C10:0
Capric
0.4 ± 0.0c
0.4 ± 0.0c

C11:0
Undecanoic
0.8 ± 0.0b
0.8 ± 0.0b

C16:0
Palmitic
3.8 ± 0.0d
3.3 ± 0.0e

C16:1
Palmitoleic
0.1 ± 0.0c
0.1 ± 0.0c

C18:0
Strearic
1.1 ± 0.03ab
0.8 ± 0.0b

C18:1n9c
Oleic***
10.3 ± 0.06g
8.9 ± 0.0h

C18:2n6c
Linoleic**
5.0 ± 0.01f
4.5 ± 0.0f

C20:2
Eicosadienoic**
0.3 ± 0.01c
0.3 ± 0.0c

C22:0
Beheric
0.1 ± 0.0c
0.1 ± 0.0c

C24:0
Lignoceric
0.4 ± 0.01c
0.4 ± 0.0c

 **Omega-6 PUFAs; ***Omega-9 PUFAs. Each value is expressed as mean ± SD (). In each row, the different letters represent significant differences between samples ().

3.4. Determination of Total Polysaccharides, Total Phenolic, and Total Flavonoids Compound

The total polysaccharides of the commercial and wild P. giganteus were comparable. The aqueous extract had the highest amount of polysaccharides as shown in Table 4. Notably, the total polysaccharides in aqueous extract of the wild strains (%, w/w) were significantly () higher than those of the commercial strain (%). On the other hand, the total phenolic content in the ethanol extracts was higher. The quantity of phenolics in the mushrooms extracts was in descending order: commercial strain ethanol extract > wild strain ethanol extract > commercial extract aqueous extract > wild strain aqueous extract. There was also a significant difference () in the total flavonoids between the basidiocarps of the commercial and wild strains. The wild P. giganteus had approximately 2.09% higher flavonoids than the commercial strains.

Table 4: Total polysaccharides, phenolics, and flavonoids present in the crude aqueous and ethanol extracts of basidiocarps of Pleurotus giganteus.

Pleurotus giganteus (commercial strain)
Pleurotus giganteus (wild strain)

Aqueous
Ethanol
Aqueous
Ethanol

Extraction yield (%, w/w)
15.60 ± 2.20a
12.00 ± 1.00b
13.77 ± 1.68ab
6.67 ± 1.06c

Total polysaccharides (%, w/w)
14.93 ± 0.04a
11.31 ± 0.16b
17.91 ± 0.05c
13.72 ± 0.04d

Total phenolic content (mg GAE/g)
12.14 ± 1.89a
24.08 ± 1.04b
9.58 ± 0.18c
21.61 ± 1.47d

Total flavonoids (mg RE/g)
ND
2.94 ± 0.00a
ND
6.14 ± 0.01b

In each row, the different letters represent significant differences between samples (). ND = not detected.

3.5. Evaluation of Antioxidant Activity of Mushroom Extracts

3.5.1. DPPH Scavenging Activity Assay

The scavenging effect of the mushroom extracts on DPPH radicals increased with sample concentration, depending on the extraction solvent and strain type. In general, the ethanol extracts showed higher scavenging activity, hence lower IC₅₀ (mg/mL) when compared to the aqueous extracts (Table 5). The scavenging activity obtained in descending order was wild strain ethanol extract > commercial strain ethanol extract > wild strain aqueous extract > commercial strain aqueous extract.

Table 5: Antioxidant activities of the aqueous and ethanol extracts from the commercial strain of Pleurotus giganteus (KLU-M 1227) and the wild strain (KLU-M 1228).

Antioxidant properties
Test method
Positive control (BHT)
Pleurotus giganteus
Pleurotus giganteus

(commercial strain)
(wild strain)

Aqueous
Ethanol
Aqueous
Ethanol

Free radical scavenging
DPPH (IC₅₀; mg/mL)
0.09 ± 0.01
21.46 ± 6.95a
11.28 ± 3.54bc
16.18 ± 1.76ab
8.10 ± 2.15c

Reducing power
FRAP (µM FeSO₄·7H₂O/ g)
780.29 ± 13.4
2.26 ± 0.29ab
2.99 ± 0.14b
2.04 ± 0.32a
2.69 ± 0.71b

Lipid peroxidation inhibition
Inhibition of lipid peroxidation at extract concentration of 10 mg/mL (%)
79.07 ± 2.25
44.41 ± 1.00a
49.58 ± 1.87b
44.61 ± 1.42a
49.80 ± 3.27b

In each row, the different letters represent significant differences between samples (P < 0.05).

3.5.2. FRAP (Ferric Reducing Antioxidant Power) Assay

In the ferric reducing power assay, the reducers present in the mushroom extracts lead to the reduction of the Fe³⁺/ferricyanide complex to the ferrous form. The reducing capacity of the mushroom extract serves as an index of antioxidant activity. The reducing ability of the different extracts was in the range of 1.17–3.88 μM FeSO₄·7H₂O/g mushroom (Table 5). The antioxidant activity obtained in descending order was commercial strain ethanol extract ≥ wild strain ethanol extract ≥ commercial strain aqueous extract ≥ commercial strain aqueous extract.

3.5.3. Inhibition of Lipid Peroxidation

The study of lipid peroxidation (LPO) inhibition is based on the measurement of malondialdehyde (MDA) generated by the polyunsaturated fatty acid peroxides upon decomposition. As a result of LPO, destruction of cellular components occurs and brings about oxidative stress in biological systems. As shown in Table 5, there was no significant difference () in terms of lipid peroxidation inhibition between the commercial and domesticated wild mushroom extracts. However, the ethanol extracts of both the strains showed significantly higher () lipid peroxidation inhibitory ability (49.58–49.80%) when compared to the aqueous extracts (44.41–44.61%). Therefore, the means of extractions instead of mushroom strains played a more prevailing role in lipid peroxidation inhibition.

3.6. Correlation between Total Polysaccharides, TPC, and Antioxidant Parameters

The TPC in the mushroom extract was positively correlated to the DPPH scavenging capacity () and FRAP reducing power (). This indicated that the antioxidant effects increased with increasing concentrations of the total phenolics present in the mushroom extracts. However, a weak correlation was found between the lipid peroxidation inhibition activity and the TPC (). Nevertheless, the DPPH scavenging capacity, FRAP reducing power, and lipid peroxidation inhibition showed a strong positive correlation ( to +.820) between each antioxidant activity.

3.7. Neurite Outgrowth Assay

The mean value of neurite-bearing cells in NGF treated cells (positive control) was % as shown in Figure 1(a). The ethanol extracts of the commercial and wild P. giganteus (20 μg/mL) caused a significant () increase in neurite-bearing cells by 3.98- and 4-fold, respectively, when compared to the control cells with medium only. Additionally, in order to verify the neuritogenic activity of these mushroom extracts, PC12 cells, which only extend neurite upon NGF activation, were employed. Incubation of PC12 with 20 μg/mL of ethanol extracts resulted in a significant increase () in neurite-bearing cells compared to cells treated with NGF alone. Phase-contrast micrographs of neurite-bearing cells were shown in Figure 1(b). The two most abundant fatty acids in the investigated extract were oleic acid and linoleic acid (Table 3). Therefore, the neuronal cells were treated with linoleic acid and oleic acid and the neurite outgrowth activity was examined. Linoleic acid enhanced the neuritogenic activity of PC12 and N2a cells significantly () but not oleic acid (Figure 1(a)).

(a)

(b)

Figure 1: (a) Neurite-bearing cells (%) after 3-day incubation with NGF, mushroom extracts, linoleic acid, and oleic acid. AE: aqueous extract; EE: ethanol extract. Data are expressed as mean ± SD of three experiments. Different letters represent significant differences between samples (). (b) Phase-contrast photomicrographs showing the effects of NGF, ethanol extract, and linoleic acid on the morphology of PC12 and differentiating N2a cells after 3 days. Untreated cells serve as control and only contained 5% FBS as vehicle. Arrows indicate neurite extension and scale bar corresponds to 20 μm.

3.8. NGF Measurement

We measured the level of NGF in the culture medium after PC12 cells were cultivated in the presence of P. giganteus extracts and linoleic and oleic acids for 3 days. Linoleic acid significantly () augmented NGF secretion by 1.4 times (Figure 2). On the other hand, oleic acid had a lower potency in promoting NGF secretion (255 pg/mL), as compared to linoleic acid (323 pg/mL). In contrast to the fatty acids, the extracts had a weak stimulatory effect on NGF secretion.

Figure 2: The effects of mushroom aqueous and ethanol extracts on the stimulation of NGF secretion by neurite-bearing PC12 cells in the presence or absence of NGF. NGF (50 ng/mL) was used as the positive control. Values are mean ± SD from three independent experiments. Different letters represent significant differences between samples (). represents a significant difference from the control, that is, 50 ng/mL of NGF.

4. Discussion

The nutritional components found in the present work are in accordance with the literature. A study in Italy showed that the protein content in Pleurotus ostreatus (grey oyster mushroom) was  g/100 g, which is lower than the protein content of P. giganteus in this study [17]. Pleurotus giganteus also showed a higher protein content when compared to Pleurotus sajor-caju (13.0–18.4 g/100 g) [18]. The protein content of mushrooms is dependent on the strain, substrate chemical composition, pileus size, and cultivation time [19, 20]. In this study, rice bran supplementation may have increased the soluble protein content present in both strains as the protein content in rice bran is about 10–15% of the total weight of the mushroom growth substrate [21].

Pleurotus ostreatus was composed mainly of glucose (14.29 g kg⁻¹) and mannose (10.55 g kg⁻¹) [22]. While fructose was not detected in P. ostreatus, 27.81 g kg⁻¹ of glucose was detected in P. eryngii with trace amounts of ribose and xylose. Pleurotus spp. in particular are rich in calcium, potassium, magnesium, iron, and phosphorus [23]. Pleurotus sajor-caju, P. platypus, and P. citrinopileatus were reported to contain , , and  g kg⁻¹ of potassium, respectively. The concentrations of vitamin B3 in mushrooms were highly species dependent and they vary from 34 to 109 mg/100 g dry weight for P. ostreatus [24]. Mushrooms contain higher vitamin B2 (riboflavin) as compared to vegetables. Some varieties of Agaricus bisporus (white button mushroom) have also been reported to contain vitamin B2 levels as high as those found in eggs and cheese [25]. In our study, vitamin B1 (thiamin) was higher in the commercial strain by 1.9-fold, while vitamin B2 level was not significantly different when compared to that of the wild strain.

The free amino acids in P. giganteus were comparable with reported literature values. Tanzanian wild mushrooms Lactarius sp. (milky cap), Boletus pruinatus (Matt Bolete mushroom), and Boletinus cavipes were reported to have leucine as high as 15.9%, 10.6%, and 8.40%, respectively [26]. The fresh P. ostreatus was found to have 2.74, 1.76, and 1.43 g kg⁻¹ of glutamine, asparagine, and arginine, respectively [27]. Similar to our findings, other edible mushrooms also contained high levels of oleic acid (% total fatty acid methyl esters): Auricularia polytricha (27.1), Lentinus sajor-caju (23.5), Lentinus squarrosulus (5.8), Pleurotus djamor (28.8), P. sajor-caju (16.4), and Russula brevipes (39.2) [28]. The presence of caprylic and capric acids in mushrooms is rare. Calocybe gambosa (St. George’s mushroom) and Clitocybe odora (Aniseed funnel mushroom) were reported to have 0.25 ± 0.02 and 0.03 ± 0.00% of caprylic acid, respectively, while Coprinus comatus (shaggy mane mushroom) registered 0.09 ± 0.00% of capric acid [29].

Extraction with boiling water is used to obtain extracts with high molecular weight compounds, such as polysaccharides which play an important medicinal role in mushrooms [30]. Low molecular weight compounds, such as phenolic compounds, were usually from ethanol extraction [29]. Although flavonoid was not detected in P. sajor-caju [31], its concentration ranging from 0.24 to 0.32 mg/g was reported in P. ostreatus [32]. Flavonoids were also present in other mushroom species like Clitocybe gibba (3.56 mg chatequin/g extract) and Boletus armeniacus (8.59 mg chatequin/g extract) [33]. The rubber wood sawdust which is rich in lignin could have been depolymerized by the lignocellulosic enzymes of mushrooms into phenolic units and further dimerized or polymerized, creating flavonoids [34].

DPPH radical scavenging effects of the mycelia extracts of Pleurotus spp. (P. citrinopileatus, P. djamor, P. eryngii, P. flabellatus, P. florida, P. ostreatus, and P. sajor-caju) have been reported [32, 35]. The methanol and hot water extracts (10 mg/mL) of P. eous were found to scavenge DPPH radical by 85.19% and 70.21%, respectively [36]. The higher DPPH scavenging ability of ethanol extracts might be due to more hydrogen-donating components including phenolic compounds extracted from the mushroom. The higher phenolic content of ethanol extracts might account for the better results found in reducing power as compared to the aqueous extract [37]. When comparing the FRAP values of P. giganteus with P. sajor-caju, FRAP values of aqueous extract of P. giganteus were lower than those of P. sajor-caju (35.06 ± 0.86 μM FeSO₄·7H₂O/g mushroom) [31]. The higher phenolic content in the ethanol extracts might contribute to a higher inhibitory effect on lipid peroxidation. Pleurotus florida was reported to display 57% of lipid peroxidation inhibition, while P. flabellatus, P. cystidiosus, P. eryngii, and P. sajor-caju showed 50%, 49.8%, 48%, and 43% of lipid peroxidation inhibition, respectively [38]. Meanwhile, P. ostreatus at a concentration of 10 mg/mL inhibited LPO activity in rat liver homogenate by 56.20% [39]. In accordance with the present results, Dubost et al. [34] had demonstrated a positive correlation between the TPC in the mushroom extracts of P. ostreatus and P. eryngii and the antioxidant capacity.

It has been reported that tiger’s milk mushroom, Lignosus rhinocerotis, demonstrated neuritogenic effects on PC12 cells [40]. The aqueous extract of Ganoderma neo-japonicum (50 μg/mL) was found to trigger a maximal stimulation of PC12 neurite outgrowth with 14.22 ± 0.43% of neurite-bearing cells [41]. Extension of neurites from neuronal cell body is an important step in neuronal development and requires the generation of additional plasma membrane [42]. Polyunsaturated fatty acids like linoleic, linolenic, docosahexanoic, and arachidonic acids promoted basal and nerve growth factor- (NGF-) induced neurite extension of the PC12. This suggested that linoleic acid which was present in abundance in the extracts of P. giganteus may play a key role in neuritogenesis. In contrast, studies showed that monounsaturated fatty acids and saturated long-chain fatty acids like oleic, stearic, and palmitic acids caused little or no effects in neurite outgrowth [43]. Studies have shown that cyathane diterpenes from the mushroom Sarcodon cyrneus, namely, cyrneines A, B, C, and D, and glaucopine C increased the NGF gene expression in 1321N1 astrocytoma cells [44]. Hericenones C, D, and E isolated from the basidiocarps of Hericium erinaceus exhibited stimulating activity for the biosynthesis of NGF in vitro. In the presence of hericenones C, D, and E at 33 μg/mL, mouse astroglial cells secreted , , , and  pg/mL of NGF into the culture medium, respectively [45].

Long-chain polyunsaturated fatty acids (LCPUFA) are essential nutrients in the development and functioning of the brain and central nervous system. The most abundant LCPUFA in the brain are docosahexaenoic acid (DHA) which is mainly derived from fish and arachidonic acid (ARA) from meat and eggs [46]. The desaturation and elongation of linoleic acids and alpha-linolenic acids to ARA and DHA, respectively, are very crucial for the infant’s brain development. Since the percentage of ARA decreases in the brain during prenatal development, the balance in the dietary ratio of linoleic acid is very crucial to the brain development in preterm infant [47]. Apart from that, many studies have demonstrated the importance of LCPUFA as potent neuroprotectant. Linoleic acid was found to protect mouse cortical neurons against glutamate excitotoxicity [48]. Linoleic acid and its derivatives also prevented sodium nitroprusside-induced cell death of cultured rat cerebral cortical neurons [49]. However, to date, limited information is available on the role of polyunsaturated fatty acid as a stimulator of NGF synthesis in neuronal cells.

5. Conclusion

In conclusion, we report for the first time the chemical compositions of the commercial and domesticated wild strains of P. giganteus. The extracts of both the strains showed distinctive antioxidant activities due to the differential distributions of total phenolics and flavonoids. Aqueous extracts did not contain flavonoids and had a lower phenolic content, hence explaining its lower antioxidative capacity. Our study demonstrates that the ethanol extract and its major constituent, linoleic acid, induced neurite outgrowth and increased NGF biosynthesis. These findings provide support for the possible role of P. giganteus as a functional food to maintain neuronal differentiation and neuritogenesis, as well as a healthy NGF supply in central and peripheral nervous system.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Acknowledgments

The authors wish to thank Kwan Yun Ha and Nurulhuda Mahamud for their help in collecting and domesticating the wild P. giganteus. This research is supported by UM High Impact Research Grant UM-MOHE UM.C/625/1/HIR/MOHE/SC/02 from the Ministry of Higher Education Malaysia. The authors also thank University of Malaya for Postgraduate Research Grant (PV007/2012A) and UMRG RP 005B-13 AFR.

References

A. P. Wasilewska-Sampaio, M. S. Silveira, O. Holub et al., “Neuritogenesis and neuronal differentiation promoted by 2,4-dinitrophenol, a novel anti-amyloidogenic compound,” The FASEB Journal, vol. 19, no. 12, pp. 1627–1636, 2005.

S. I. Liochev, “Reactive oxygen species and the free radical theory of aging,” Free Radical Biology and Medicine, vol. 60, pp. 1–4, 2013.

C.-W. Phan, P. David, M. Naidu, K.-H. Wong, and V. Sabaratnam, “Therapeutic potential of culinary-medicinal mushrooms for the management of neurodegenerative diseases: diversity, metabolite, and mechanism,” Critical Reviews in Biotechnology, 2014.

S. J. Allen, J. J. Watson, D. K. Shoemark, N. U. Barua, and N. K. Patel, “GDNF, NGF and BDNF as therapeutic options for neurodegeneration,” Pharmacology & Therapeutics, vol. 138, no. 2, pp. 155–175, 2013.

N. Klomklung, S. C. Karunarathna, E. Chukeatirote, and K. D. Hyde, “Domestication of wild strain of Pleurotus giganteus,” Sydowia, vol. 64, no. 1, pp. 39–53, 2012.

W. Wong, M. A. Abdulla, K. Chua, U. R. Kuppusamy, Y. Tan, and V. Sabaratnam, “Hepatoprotective effects of Panus giganteus (Berk.) corner against thioacetamide-(TAA-) induced liver injury in rats,” Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 170303, 10 pages, 2012.

C.-W. Phan, G.-H. Lee, I. G. Macreadie, S. Malek, D. Pamela, and V. Sabaratnam, “Lipid constituents of the edible mushroom, Pleurotus giganteus demonstrate anti-Candida activity,” Natural Product Communications, vol. 8, pp. 1763–1765, 2013.

C.-W. Phan, W. Wong, P. David, M. Naidu, and V. Sabaratnam, “Pleurotus giganteus (Berk.) Karunarathna & K.D. Hyde: Nutritional value and in vitro neurite outgrowth activity in rat pheochromocytoma cells,” BMC Complementary and Alternative Medicine, vol. 12, article 102, 2012.

C.-W. Phan, P. David, M. Naidu, K.-H. Wong, and V. Sabaratnam, “Neurite outgrowth stimulatory effects of culinary-medicinal mushrooms and their toxicity assessment using differentiating Neuro-2a and embryonic fibroblast BALB/3T3,” BMC Complementary Alternative Medicine, vol. 13, p. 261, 2013.

AOAC, Official Methods of Analysis, Association of Official Analytical Chemists, Arlington, Va, USA, 16th edition, 1995.

M. Dubois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith, “Colorimetric method for determination of sugars and related substances,” Analytical Chemistry, vol. 28, no. 3, pp. 350–356, 1956.

S. K. Singdevsachan, J. K. Patra, and H. Thatoi, “Nutritional and bioactive potential of two wild edible mushrooms (Lentinus sajor-caju and Lentinus torulosus) from Similipal Biosphere Reserve, India,” Food Science and Biotechnology, vol. 22, no. 1, pp. 137–145, 2013.

H. I. Ismail, K. W. Chan, A. A. Mariod, and M. Ismail, “Phenolic content and antioxidant activity of cantaloupe (Cucumis melo) methanolic extracts,” Food Chemistry, vol. 119, no. 2, pp. 643–647, 2010.

S. Gorinstein, J. Drzewiecki, H. Leontowicz et al., “Comparison of the bioactive compounds and antioxidant potentials of fresh and cooked Polish, Ukrainian, and Israeli garlic,” Journal of Agricultural and Food Chemistry, vol. 53, no. 7, pp. 2726–2732, 2005.

I. F. F. Benzie and J. J. Strain, “Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration,” Methods in Enzymology, vol. 299, pp. 15–27, 1999.

U. R. Kuppusamy, M. Indran, and B. R. S. Balraj, “Antioxidant effects of local fruits and vegetable extracts,” Journal of Tropical Medicinal Plants, vol. 3, pp. 47–53, 2002.

P. Manzi, A. Aguzzi, and L. Pizzoferrato, “Nutritional value of mushrooms widely consumed in Italy,” Food Chemistry, vol. 73, no. 3, pp. 321–325, 2001.

M. Bonatti, P. Karnopp, H. M. Soares, and S. A. Furlan, “Evaluation of Pleurotus ostreatus and Pleurotus sajor-caju nutritional characteristics when cultivated in different lignocellulosic wastes,” Food Chemistry, vol. 88, no. 3, pp. 425–428, 2004.

M. N. Shashirekha, S. Rajarathnam, and Z. Bano, “Effects of supplementing rice straw growth substrate with cotton seeds on the analytical characteristics of the mushroom, Pleurotus florida (Block & Tsao),” Food Chemistry, vol. 92, no. 2, pp. 255–259, 2005.

M. D. Nunes, J. Maria, S. A. Paes, J. Júlio, and O. Ribeiro, “Nitrogen supplementation on the productivity and the chemical composition of oyster mushroom,” Journal of Food Research, vol. 1, pp. 113–119, 2012.

C. Fabian and Y. Ju, “A review on rice bran protein: its properties and extraction methods,” Critical Reviews in Food Science and Nutrition, vol. 51, no. 9, pp. 816–827, 2011.

M.-Y. Kim, L.-M. Chung, S.-J. Lee et al., “Comparison of free amino acid, carbohydrates concentrations in Korean edible and medicinal mushrooms,” Food Chemistry, vol. 113, no. 2, pp. 386–393, 2009.

R. Ragunathan and K. Swaminathan, “Nutritional status of Pleurotus spp. grown on various agro-wastes,” Food Chemistry, vol. 80, no. 3, pp. 371–375, 2003.

P. C. K. Cheung, “The nutritional and health benefits of mushrooms,” Nutrition Bulletin, vol. 35, no. 4, pp. 292–299, 2010.

P. Mattila, K. Könkö, M. Eurola et al., “Contents of vitamins, mineral elements, and some phenolic compounds in cultivated mushrooms,” Journal of Agricultural and Food Chemistry, vol. 49, no. 5, pp. 2343–2348, 2001.

S. J. M. Mdachi, M. H. H. Nkunya, V. A. Nyigo, and I. T. Urasa, “Amino acid composition of some Tanzanian wild mushrooms,” Food Chemistry, vol. 86, no. 2, pp. 179–182, 2004.

G. Jaworska, E. Bernaś, and B. Mickowska, “Effect of production process on the amino acid content of frozen and canned Pleurotus ostreatus mushrooms,” Food Chemistry, vol. 125, no. 3, pp. 936–943, 2011.

S. Kavishree, J. Hemavathy, B. R. Lokesh, M. N. Shashirekha, and S. Rajarathnam, “Fat and fatty acids of Indian edible mushrooms,” Food Chemistry, vol. 106, no. 2, pp. 597–602, 2008.

J. A. Vaz, L. Barros, A. Martins, C. Santos-Buelga, M. H. Vasconcelos, and I. C. F. R. Ferreira, “Chemical composition of wild edible mushrooms and antioxidant properties of their water soluble polysaccharidic and ethanolic fractions,” Food Chemistry, vol. 126, no. 2, pp. 610–616, 2011.

I. C. F. R. Ferreira, J. A. Vaz, M. H. Vasconcelos, and A. Martins, “Compounds from wild mushrooms with antitumor potential,” Anti-Cancer Agents in Medicinal Chemistry, vol. 10, no. 5, pp. 424–436, 2010.

G. Kanagasabapathy, S. N. A. Malek, U. R. Kuppusamy, and S. Vikineswary, “Chemical composition and antioxidant properties of extracts of fresh fruiting bodies of Pleurotus sajor-caju (Fr.) Singer,” Journal of Agricultural and Food Chemistry, vol. 59, no. 6, pp. 2618–2626, 2011.

E. Vamanu, “In vitro antioxidant and antimicrobial activities of two edible mushroom mycelia obtained in the presence of different nitrogen sources,” Journal of Medicinal Food, vol. 16, no. 2, pp. 155–166, 2013.

E. Pereira, L. Barros, A. Martins, and I. C. F. R. Ferreira, “Towards chemical and nutritional inventory of Portuguese wild edible mushrooms in different habitats,” Food Chemistry, vol. 130, no. 2, pp. 394–403, 2012.

N. J. Dubost, B. Ou, and R. B. Beelman, “Quantification of polyphenols and ergothioneine in cultivated mushrooms and correlation to total antioxidant capacity,” Food Chemistry, vol. 105, no. 2, pp. 727–735, 2007.

K. K. Mishra, R. S. Pal, R. Arunkumar, C. Chandrashekara, S. K. Jain, and J. C. Bhatt, “Antioxidant properties of different edible mushroom species and increased bioconversion efficiency of Pleurotus eryngii using locally available casing materials,” Food Chemistry, vol. 138, no. 2-3, pp. 1557–1563, 2013.

G. Sudha, S. Vadivukkarasi, R. B. I. Shree, and P. Lakshmanan, “Antioxidant activity of various extracts from an edible mushroom pleurotus eous,” Food Science and Biotechnology, vol. 21, no. 3, pp. 661–668, 2012.

V. L. Singleton and J. A. J. Rossi, “Colorimetric of total phenolics with phosphomolybdic-phosphotungstic acid reagents,” American Journal of Enology and Viticulture, vol. 16, pp. 144–158, 1965.

N. Abdullah, S. M. Ismail, N. Aminudin, A. S. Shuib, and B. F. Lau, “Evaluation of selected culinary-medicinal mushrooms for antioxidant and ACE inhibitory activities,” Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 464238, 12 pages, 2012.

T. Jayakumar, P. A. Thomas, and P. Geraldine, “In-vitro antioxidant activities of an ethanolic extract of the oyster mushroom, Pleurotus ostreatus,” Innovative Food Science and Emerging Technologies, vol. 10, no. 2, pp. 228–234, 2009.

P. A. John, K. H. Wong, M. Naidu, and V. Sabaratnam, “Combination effects of curcumin and aqueous extract of Lignosus rhinocerotis mycelium on neurite outgrowth stimulation activity in PC-12 cells,” Natural Product Communications, vol. 8, pp. 711–714, 2013.

S. Ling-Sing Seow, M. Naidu, P. David, K. Wong, and V. Sabaratnam, “Potentiation of neuritogenic activity of medicinal mushrooms in rat pheochromocytoma cells,” BMC Complementary and Alternative Medicine, vol. 13, article 157, 2013.

Y. Kamata, H. Shiraga, A. Tai, Y. Kawamoto, and E. Gohda, “Induction of neurite outgrowth in PC12 cells by the medium-chain fatty acid octanoic acid,” Neuroscience, vol. 146, no. 3, pp. 1073–1081, 2007.

F. Darios and B. Davletov, “Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3,” Nature, vol. 440, no. 7085, pp. 813–817, 2006.

M. C. Marcotullio, R. Pagiotti, F. Maltese et al., “Cyathane diterpenes from Sarcodon cyrneus and evaluation of their activities of neuritegenesis and nerve growth factor production,” Bioorganic & Medicinal Chemistry, vol. 15, no. 8, pp. 2878–2882, 2007.

H. Kawagishi, M. Ando, H. Sakamoto et al., “Hericenones C, D and E, stimulators of nerve growth factor (NGF)-synthesis, from the mushroom Hericium erinaceum,” Tetrahedron Letters, vol. 32, no. 35, pp. 4561–4564, 1991.

C. I. F. Janssen and A. J. Kiliaan, “Long-chain polyunsaturated fatty acids (LCPUFA) from genesis to senescence: the influence of LCPUFA on neural development, aging, and neurodegeneration,” Progress in Lipid Research, vol. 53, pp. 1–17, 2014.

U. Enke, A. Jaudszus, E. Schleussner, L. Seyfarth, G. Jahreis, and K. Kuhnt, “Fatty acid distribution of cord and maternal blood in human pregnancy: special focus on individual trans fatty acids and conjugated linoleic acids,” Lipids in Health and Disease, vol. 10, article 247, 2011.

W. T. Hunt, A. Kamboj, H. D. Anderson, and C. M. Anderson, “Protection of cortical neurons from excitotoxicity by conjugated linoleic acid,” Journal of Neurochemistry, vol. 115, no. 1, pp. 123–130, 2010.

T. Yaguchi, H. Fujikawa, and T. Nishizaki, “Linoleic acid derivative DCP-LA protects neurons from oxidative Stress-Induced apoptosis by inhibiting caspase-3/-9 activation,” Neurochemical Research, vol. 35, no. 5, pp. 712–717, 2010.