Optimal Immobilization of β-Galactosidase onto κ-Carrageenan Gel Beads Using Response Surface Methodology and Its Applications

β-Galactosidase (β-gal) was immobilized by covalent binding on novel κ-carrageenan gel beads activated by two-step method; the gel beads were soaked in polyethyleneimine followed by glutaraldehyde. 22 full-factorial central composite experiment designs were employed to optimize the conditions for the maximum enzyme loading efficiency. 11.443 U of enzyme/g gel beads was achieved by soaking 40 units of enzyme with the gel beads for eight hours. Immobilization process increased the pH from 4.5 to 5.5 and operational temperature from 50 to 55°C compared to the free enzyme. The apparent K m after immobilization was 61.6 mM compared to 22.9 mM for free enzyme. Maximum velocity V max was 131.2 μmol·min−1 while it was 177.1 μmol·min−1 for free enzyme. The full conversion experiment showed that the immobilized enzyme form is active as that of the free enzyme as both of them reached their maximum 100% relative hydrolysis at 4 h. The reusability test proved the durability of the κ-carrageenan beads loaded with β-galactosidase for 20 cycles with retention of 60% of the immobilized enzyme activity to be more convenient for industrial uses.


Introduction
Lactose is the main carbohydrate contained in milk at a concentration between 5% and 10% (w/v) depending on the source of milk [1]. Lactose could be also found in whey permeate at higher concentrations. The consumption of foods with a high content of lactose is causing a medical problem for almost 70% of the world population, especially in the developing countries, as the naturally present enzyme in the human intestine loses its activity during lifetime [2]. Unfortunately, there is no cure to lactose intolerance. This fact, together with the relatively low solubility and sweetness of lactose, has led to an increasing interest in the development of industrial processes to hydrolyze the lactose contained in dairy products (milk and whey) with both the free and immobilized conditions [3]. The studies have shown that glucose and galactose, two monosaccharides hydrolyzed from lactose, are four times sweeter than lactose, more soluble, and more digestible [4] and can be consumed by "lactose intolerant" people [1,5]. Hydrolysis of lactose present in whey permeate will produce lactose-free syrup, solving an aquatic pollution problem as whey is usually thrown in water. 2 The Scientific World Journal Immobilized enzyme is more favorable than free enzyme since it offers the possibility of continuous flow processing, so that easy regeneration of the immobilized enzyme and low cost operation can be achieved in industrial processing.
-Galactosidase has been immobilized onto a wide variety of solid supports such as Sephadex, alginate, -carrageenan, chitosan, porous glass, agarose, polyvinyl alcohol polymers, diethylaminoethyl cellulose, Eupergit C (epoxy-activated acrylic beads), nylon, polyurethane foams, or zeolite [11]. Carrageenan has been used for the immobilization of enzymes and cells using entrapment techniques. It is inexpensive but suffers from weak mechanical and thermal stability [12]. Some work has been performed to improve its mechanical and thermal stability and it was found that the gels mechanical strength increased with the increased addition of 3,6-anhydro-D-galactose 2-sulphate in the polymer or after addition of gums. K + , Al 3+ was also found to improve the gels characteristics. In the field of immobilization of enzymes, -carrageenan is one of the main supports used for cell and enzyme immobilization via entrapment; for example,carrageenan was used to immobilize -chymotrypsin using an encapsulation method. However, one of the main disadvantages of these biopolymers is that they are usually used for immobilization of enzymes using noncovalent bonds (mainly entrapment/encapsulation) due to the lack of functionalities. Unfortunately, the entrapment of enzymes in hydrogels is often characterized by some diffusion of the biocatalyst from the support, particularly for enzymes with molecular weight less than 300 kDa. Recently, a few authors were successful at covalently immobilizing enzymes using hydrogels [13][14][15].
To overcome the problem of the gels' low thermal stability, gels were treated with polyamines to form a polyelectrolyte complex. According to [16] the thermal stability of -carrageenan gels could be improved by adding amine compounds and, especially, polyamine compounds. For this reason, natural polyamines such as chitosan and synthetic ones such as polyethylenimines were used to improve the carrageenan gels' thermal stabilities [17].

Experimental Techniques.
As a general rule, experiments were carried out in triplicate and data are means ± SD ( = 3) unless stated otherwise.

Determination of -Galactosidase
Activity. -Galactosidase activity was determined by the rate of glucose formation in the reaction medium. Known amount of immobilized or free enzyme was incubated into 10 mL of 200 mM lactose solution in citrate-phosphate buffer (100 mM, pH 4.5) for 3 h at 37 ∘ C. At the end of the time 50 L of reaction mixture was added to 950 L buffer and boiled for 10 min to inactivate the enzyme and analyzed for glucose content using the glucose test. One enzyme unit (IU) was defined as the amount of enzyme that catalyzes the formation of 1 mol of glucose per minute under the specified conditions [17].

Preparation of Carrageenan
Beads. -Carrageenan gel was prepared as previously reported by Elnashar and Yassin [17] by dissolving 2.5% (w/v) carrageenan in distilled water at 70 ∘ C and 0.002% (w/v) NaN 3 was added as antibacterial using an overhead mechanical stirrer until complete dissolution had occurred. Then carrageenan gel was transferred to an encapsulator ( Figure 1) to make gel beads. The carrageenan gel beads were hardened using 0.3 M KCl for 3 h.

Activation of Gel Beads.
For activation of carrageenan beads, they were soaked in polyethyleneimine (PEI) at desired concentration and left to react with it at room temperature. The unreacted PEI was then removed from the beads by successive washing with distilled water. The aminated beads (PEI-carr.) were then reacted with glutaraldehyde (GA) solution of specific concentration for selected time and temperature, and then the beads were washed with distilled water to remove unreacted GA. After that the activated beads were ready for immobilization step as shown in Scheme 1.

Enzyme Immobilization.
The concentration of the enzyme reacted with the activated gel beads has an obvious effect. The reaction occurred between the free C=O group found on glutaraldehyde and the NH 2 group found in the enzyme forming C=N-bond, Scheme 1.

Determination of Maximum Loading and Optimum Time of Immobilization Using 2 2 Full-Factorial Central Composite
Experiment Designs. In order to optimize the amount of loading units and the time of loading using gel beads treated with PEI and GA, 2 2 full-factorial central composite design [18] was applied with four-star points (±∞) and three replicates at the center point. The coded and actual values are described in Table 1.  3.6. Optimum Lactose pH. The effect of pH on the activity of the free and immobilized -gal enzyme was examined by incubation of 10 U of both enzyme forms in 10 mL of lactose solution 200 mM for 3 hrs at different pH values ranging from 2.2 to 8 pH at 37 ∘ C. The data was normalized to 100% relative activity at pH 5 for the free enzyme and at pH 6 for the immobilized enzyme. The relative activity at each pH is expressed as a percentage of the 100% activity.

3.7.
Optimum Lactose Temperature. The optimum temperature for the free and immobilized -gal was examined. 10 U of free and immobilized -gal was incubated for 3 hrs into 10 mL lactose solution 200 mM at pH 6 and temperatures from 30 to 75 ∘ C. The optimum temperature has been taken as 100% activity and the relative activity at each temperature is expressed as a percentage of the 100% activity.

Kinetic Constants of Free and Immobilized -Galactosidase.
To determine the affinity of the enzyme for its substrate, and max can be determined by using free and immobilized -gal. of 5 U for each and using different concentrations of lactose ranging from 25 to 200 mM at 37 ∘ C and pH 4.5 for 3 h.

Full Conversion.
Full conversion of lactose, lactose in milk, and also lactose in whey was determined by using 10 U of immobilized and free enzyme incubated in 10 mL of each solution at nearly the same concentration of lactose at 37 ∘ C, pH 6 for 3 hrs.

Operational Stability.
To study the reusability of immobilized -galactosidase for lactose hydrolysis in milk, whey, and lactose solution, 10 mL of milk containing 8% lactose, 10 mL of whey, and 10 mL of 200 mM lactose were incubated at 37 ∘ C and pH 6 with 5 g of immobilized -galactosidase containing 50 U.

Activation Process.
Polyethyleneimine (PEI) is a synthetic cationic polymer that contains primary, secondary, and tertiary amine groups in its skeleton. PEI has been widely used to cross-link the gel surfaces before reaction with enzymes to avoid their leakages. Polyethyleneimine (PEI) is used as an activator and spacer to make the enzyme far away from beads to be free in its reaction with the substrate and thus it reacts with the negative charge on the beads surface by its positive charge to form ionic bond as shown in Scheme 1.
Glutaraldehyde is a cheap and very efficient cross-linker and hence remains the reagent of choice for cross-linking, although many reagents and newer methods are available [19]. Glutaraldehyde is a very reactive substance and the use of increasing concentrations of it to activate a support may result in matrices with different internal structure and also affect the surface of the matrices [20]. Glutaraldehyde reacts with aminated gel beads and replaces the free NH 2 and turns it to N=C bond forming free C=O which the enzyme will attach to as shown in Scheme 1.

4
The Scientific World Journal  The results in Table 2 represent the design matrix of 11 trial experiments. For predicting the optimal point, a second-order polynomial function was fitted to correlate the relationship between independent variables (the amount of loading units and time of loading) and response ofgalactosidase activity, the polynomial equation was in the following form: where -galactosidase is the predicted activity of -galactosidase activity U/g beads, 1 and 2 are the independent variables corresponding to the loading time and amount of loading units, respectively, 0 is the intercept, 1 and 2 , are linear coefficients, 11 and 22 , are quadratic coefficients, and 12 is cross product coefficients. Statistical software SPSS (version As shown in Table 3, ANOVA for -galactosidase activity ( Beads ) immobilized onto gel beads indicated the value to be 14.549, which implied that the model is significant.  Model terms having values of Prob > (0.000) less than 0.05 were considered significant. ANOVA showed an 2 of 0.980 which was equivalent to 98.0%. These results proved that the data variability could be explained by this model. The observed 2 of 0.980 was in reasonable agreement with the adjusted 2 of 0.960, which again ensured a satisfactory adjustment of the quadratic model to the experimental data. A value of 99.9% correlation between observed and predicted results was obtained as in Table 2 for the trial number 8 that showed the highest enzyme loaded capacity of 11.302 U/g gel beads compared to 11.443 U/g gel beads for the experimental and predicted values, respectively. All the above reflected the accuracy and applicability of the central composite design for optimization of -galactosidase immobilization process. The maximum -galactosidase loading capacity of 11.443 U/g beads was obtained by loading 40 U for 8 hours. This result was very similar to the predicted value obtained by the polynomial equation (11.357 U/g beads). The recovery of the enzyme activity was almost 29%. This result is somewhere reasonable compared to other authors who recovered between 2 and 83% using immobilized -galactosidase from the same enzyme species [21].
Three-dimensional response surfaces were plotted on the basis of the model equation, to investigate the interaction among the variables and to determine the optimum value as shown in Figure 2. Figure 2 showed that higher levels of the -galactosidase activity were attained with increasing the loading units and loading time till 8 hours which is sufficient for maximum enzyme loading. Figure 3 the optimum pH for free and immobilized -gal enzyme was 4.5-5 pH, and it is also obvious that the immobilized enzyme is more stable than the free one and gives higher activity than the free one at each pH.   Figure 4 Illustrates the optimum temperature profile for the immobilized and free -gal enzyme, and from the figure the immobilized enzyme was found to be stable at a wider range of temperature (45-55 ∘ C) than the free enzyme (45-50 ∘ C). The shift of the optimum temperature towards higher temperatures is an indication of the more thermal stability for the enzyme after immobilization process.

Kinetic Constants of Free and Immobilized -Galactos-
idase. The kinetic constants of free and immobilizedgal. were calculated using the Hanes-Woolf plot method as shown in Figure 5. The calculated values are shown in Table 4. The apparent after immobilization, 61.6 mM, is higher than that of the free enzyme, 22.9 mM, which indicates that a higher concentration of substrate is needed for the immobilized enzyme compared to the free enzyme, while the maximum reaction velocity " max " value for the immobilized enzyme was higher than that of the free enzyme; that is, it increased from 131.2 mol⋅min −1 to 177.1 mol⋅min −1 . Figure 6, the relative rate of conversion of lactose to glucose using the free enzyme was higher for the free enzyme than the immobilized one for the first 3.5 hours. After which, both enzyme forms reached their   maximum relative conversion rate at around 4 h. The higher activity for the free enzyme compared to the immobilised form for the first 3.5 hours could be regarded to the time required for the immobilised enzyme to retain its better 3D conformation inside the gel beads.

Full Conversion. As shown in
Operational Stability. The stability of immobilized enzyme, easiness of separation, and capability of being reused many times are more important and advantageous for industrial enzymes. As shown in Figure 7, the immobilized -gal enzyme was used repeatedly 20 times and the residual activity was about 60% of its initial. This behavior may be related to the pH of the substrate medium.

Conflict of Interests
There is no conflict of interests regarding the publication of this paper.