Comprasion of ICare Rebound Tonometer and Goldmann Applanation Tonometer in High Myopia

Purpose. To compare intraocular pressure (IOP) measurements with the Goldmann applanation tonometer (GAT) and the ICare rebound tonometer (RBT) in high myopic eyes. Patients and Methods. This randomized prospective study included 40 eyes of 40 patients with high myopia. All patients' central corneal thickness (CCT), anterior chamber depth (ACD), axial length (AXL), keratometry, and refractive measurements were recorded and followed by IOP measurement with RBT and GAT. Results. The average CCT, AXL, and ACD were determined to be 514.65 ± 32 μm, 27.65 ± 2.22 mm, and 3.25 ± 0.51 mm, respectively. Mean K was 43.27 ± 1.4 D and mean spherical equivalent was −11.31 ± 4.30 D. The mean IOP values obtained by RBT and GAT were 17.18 ± 3.72 mmHg and 16.48 ± 3.19 mmHg, respectively. The deviations of RBT readings from corrected GAT values were highly correlated with CCT values (r = 0.588, P = 0.0001). The mean corrected GAT reading was 17.49 ± 3.01 mmHg. Linear regression analysis showed that a CCT change of 10 μm resulted in an RBT reading deviation of 0.57 mmHg. The Bland-Altman scatter-plot and McNemar test showed a clinically good level of agreement between the two tonometers. Conclusion. This study found a good agreement level between the two tonometers in high myopic patients and that RBT measurements are influenced by CCT variations.


Introduction
Elevated intraocular pressure (IOP) is the most important risk factor in glaucoma; lowering of IOP may stop or delay the progression of disease [1,2]. Goldmann applanation tonometer is widely accepted current gold standard for IOP measurement. This device has some disadvantages such as being affected by central corneal thickness (CCT), may cause local trauma to the corneal surface, may cross-contaminate and requires slit lamp biomicroscopy and topical anesthesia. Further, topical anesthesia may cause reflex blepharospasm and allergic reactions [3]. Therefore to eliminate these disadvantages, various alternative instruments have been developed [4][5][6][7]. The rebound tonometer (RBT) seems to be a good alternative, with advantages over other tonometers such as simple usage so that establishing cooperation with the patient is easy, quickly performed, highly reproducible, and portable and does not require anesthetic drops and slit lamp biomicroscopy [8][9][10][11]. After Kantiola's encouraging results with this tonometer, commercial production was started in 2003 under the name "ICare rebound tonometer" (ICare TA01; Tiolat, Helsinki, Finland) and it has entered our daily clinical practice in gradually increasing rates until the present day [8,9].
The ICare rebound tonometer, which is based on the induction rebound or impact principle, monitors the motion parameters of the probe colliding with an eye. The return rate of the probe after it touches the cornea gives information about IOP; if the return is slow, low IOP values are obtained and if it is fast, high IOP values are obtained [8].
It has been established that high myopia is a risk factor in the development and progression of glaucoma [12,13]. Marcus et al., in their review and meta-analysis involving 48161 patients, have reported that the risk of development of open-angle glaucoma increases for high myopic patients [13]. To the best of our knowledge, comparative studies on 2 The Scientific World Journal the measurement of IOP with RBT in high myopic eyes are limited [14]. The purpose of our study was to compare IOP readings taken with GAT and RBT and to assess the influence of CCT, AXL (axial length), and ACD (anterior chamber depth) on IOP measurements.

Patients and Methods
The study was conducted in accordance with the recommendations of the Declaration of Helsinki. The local medical ethics committee approved the study and informed consent for participation was obtained from each subject. This prospective and randomized study included adult patients who had no ocular pathology other than having myopia of 6 diopters or over. Exclusion criteria included presence of any ocular pathology other than high myopia and ocular hypertension.
All patients underwent a complete ophthalmologic examination, including best-corrected visual acuity evaluation, slit-lamp examination, gonioscopy, and fundus biomicroscopy with a 90-diopter lens.
The central corneal thicknesses (CCT) of all patients were measured with a central ultrasonic pachymeter (Pacline pachymeter, Optikon 2000, Italy). After the pachymeter probe was placed on the corneal center, 3 measurements were taken and the mean value was used for further analysis. The anterior chamber depth (ACD) and axial length (AXL) measurements were taken 3 times with ultrasound biometry (Bioline, Optikon 2000) and the mean value was used for further analysis. Keratometry and refractive measurements were taken with a noncontact autorefractometer (RK-F1; Canon, Tokyo, Japan).
IOP measurements with RBT and GAT were taken by two different physicians (Halil Huseyin Cagatay and Metin Ekinci), with a minimum 15-minute time interval between readings. All instruments were calibrated and measurements were performed according to instructions and guidelines of the manufacturer. The RBT software is preprogrammed for 6 measurements. After the sixth measurement, the letter P appears in the display, followed by a mean reading with a standard deviation on the screen. The RBT software discards the highest and lowest IOP readings automatically and calculates the mean IOP value from the rest. We discarded the results when the error sign appeared on the screen. After every measurement, the probe on the device was changed. After instillation of topical proparacaine anesthesia (Alcaine, Alcon, ABD), a fluorescein strip was applied and IOP measurements were taken for 3 times with the GAT (Haag Streit, Koeniz, Switzerland) and the average IOP value was determined. GAT measurements were taken by different physicians, who were blinded to the IOP measurement obtained by the RBT.
In the corrected IOP calculation, depending on the CCT, the Doughty and Zaman formula was used as follows: corrected IOP = IOP measured with GAT − ((CCT − 535) × (2.5/50)) [15]. Corrected IOP has been accepted as the gold standard. In all patients, the difference between IOP values and RBT values was compared and analyzed. The relation of   The Bland-Altman scatter-plot comparing the GAT and RBT readings (Figure 2) showed good agreement between the 2 methods. The differences between corresponding measures (RBT value minus GAT value) had a mean of 0.71 mmHg, a standard deviation of 2.35 mmHg, and a 95% confidence interval of −5.3 to 3.9 mmHg. These differences appeared to be nonsignificant, as shown in the plot ( = 0.097) in Figure 2.
According to the RBT readings of IOP, 30.0% of the subjects were within ±1 mmHg of IOP performed with the GAT, 67.5% of the subjects were within ±2 mmHg, and 82.5% of the subjects were within ±3 mmHg. ICare rebound tonometer identified an IOP of 21 mmHg or above in 5 of 6 subjects with a GAT identified IOP equal to or above 21 mmHg (RBT has a sensitivity of 83.3%). As well, RBT identified an IOP under 21 mmHg in 33 of 34 subjects having a GAT IOP under 21 mmHg (RBT has a specificity of 97.1%). Power of this study was 0.85 and effect size was 0.40.

Discussion
Accurate and reliable measurement of intraocular pressure is extremely important in the diagnosis and follow-up of the glaucoma. Various measurement methods have been used previously in the determination of IOP [4]. In previous studies, it has been shown that the RBT gives higher results than the GAT, especially in adult patients, and it has been mentioned by many authors that there is a reasonable agreement between the RBT and the GAT, which is accepted as the gold standard in IOP measurement for various patient groups [16][17][18][19][20][21][22][23]. Also, a medium or high level of correlation between the GAT and the RBT results has been reported. Previous studies were conducted with pathologic corneas, healthy eyes, pediatric, or glaucoma subjects and there is limited literature on high myopic patients.
Our study aimed to determine the reliability of the RBT in patients with myopia higher than 6 D, its correlation level with the GAT, and the influence of CCT, AXL, and ACD on the results for each measurement method. Chui et al. reported no significant difference in measurements made from the 4 The Scientific World Journal central and temporal/peripheral cornea depending on CCT and there was no correlation between RBT measurements and CCT values [24]. In our study, in eyes having myopia of 6 dioptry or over the good level of clinical agreement that has been determined with the GAT was independent of the RBT's axial lengths.
In our study, in accordance with the literature, the RBT measurements were affected by CCT and every 10 m change in CCT level caused a 0.57 mmHg change in the intraocular pressure obtained with RBT ( < 0.001) [25,26]. In addition to the influence of CCT on IOP measurements obtained with the RBT, this tonometer has been shown not to be significantly correlated with ACD, AXL, keratometry, and refractive values.
Limitations of this study were the relatively small number of eyes for IOP analysis and did not measure the hysteresis of the participant corneas.
To the best of our knowledge, comparative studies on the measurement of IOP with RBT in high myopic eyes are limited. Avitabile et al. in their study evaluated the refractive errors on IOP measurements with Icare and Goldmann applanation tonometry [14]. Although they did not classify the level of the myopia, they reported that in myopic eyes, the RT-GAT difference is correlated with the refraction, but not with the CCT. In controversy of this finding, we did not obtain a correlation with refraction and RT-GAT difference in high myopic eyes.
In our study, there was no significant difference between average IOP values obtained with the RBT and the GAT in high myopic eyes. It was also determined that the RBT measurements were affected by CCT and there was a clinically acceptable correlation between the two devices as determined by linear regression analysis and Bland-Altman analysis. We suggest that the RBT can be used in IOP measurement by being combined with pachymeter measurements in high myopia patients. Studies involving more patients or intraocular manometric measurements are needed to determine the reliability of the RBT in high myopic patients.