ISRN ZoologyVolume 2012 (2012), Article ID 325064, 6 pagesdoi:10.5402/2012/325064

Research Article

A New Species of the Genus Garra Hamilton, 1822 from the Chindwin Basin of Manipur, India (Teleostei: Cyprinidae: Labeoninae)

Bungdon Shangningam and Waikhom Vishwanath

Department of Life Sciences, Manipur University, Manipur, Canchipur 795003, India

Received 18 November 2011; Accepted 14 December 2011

Academic Editors: M. Cucco, J. Gautrais, and L. Kaczmarek

Copyright © 2012 Bungdon Shangningam and Waikhom Vishwanath. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Garra namyaensis sp. nov., a new cyprinid fish, is described from the Chindwin River basin in Manipur, India. It is distinguished from its congeners in having a unique combination of the following characters: smoothly rounded snout tip with a prominent rostral lobe, chest and abdominal region with scales, dorsal fin with a black band near the posterior margin, and caudal fin with a distinct W-shaped black band.

1. Introduction

Fishes of the genus Garra Hamilton, 1822 are characteristic in having a more or less well-developed suctorial disc on the undersurface just behind the mouth. These fishes inhabit rapid running waters and maintain themselves against swift currents by clinging to the substratum, mainly by means of their suctorial disc but also by the horizontally placed paired fins, especially the pectorals [1]. The genus is widely distributed from Southern China, across Southeast Asia, India, and the Middle East to Northern and Central Africa [2]. There are more than 170 nominal species of Garra [3].

Recent collection of freshwater fishes from the Namya River of Ukhrul district, Manipur, Chindwin basin, included an undescribed species of Garra, which is described herein as Garra namaensis sp. nov.

2. Materials and Methods

The description is based on formalin-preserved specimens. Counts, measurements, and terminology follow Kullander and Fang [2], and that of head depth follows Menon [1]. Measurements were taken point to point with dial calliper to the nearest 0.1 mm. The number in round brackets of parentheses after a specific count indicates the number of specimens examined. Terminology used for description of disc is reported by Zhang et al. [4]. Fin rays and numbers of scales were counted under a stereo-zoom transmitted light microscope. Lateral line scale is counted from the anteriormost pored scale in contact with the shoulder girdle to the last posteriormost pored scale on the caudal fin. Transverse-scale rows above the lateral line are counted from the dorsal-fin origin to lateral line obliquely ventrad and caudad, and those below lateral line, from the pelvic-fin origin obliquely dorsad and rostrad to lateral line. Osteological structures were observed in a cleared and alizarin-stained specimen. Vertebral counts follow Roberts [5], and scale counts follow Kottelat [6]. Morphometric measurement data are given in percentages of standard length (SL), head length (HL), pelvic-anal distance, and head and caudal peduncle depth. Specimens examined for the study are deposited in the Manipur University Museum of Fishes (MUMF).

2.1. Taxonomy

Garra namyaensis sp. nov. (Figure 1)

Figure 1: Garra namaensis, new species, holotype: (1) side view; (2) side view of snout showing rostral lobe; (3) mental disc.

2.1.1. Type Material

HolotypeIndia, Manipur state, Ukhrul district, Namya River, close to Indo-Myanmar border, 72.5 mm SL; 24°52′N 94°39′E; Shangningam et al., 18.IV.2011 (MUMF 12042).

Paratypes4 spec. same data as holotype, 28.1–53.9 mm SL (MUMF 12041,12043, 12044, 12045), one paratype (30.0 mm SL) dissected for osteology.

Comparative MaterialGarra abhoyaiMUMF 6296–6305, 10 spec. 49.3–54.90 mm SL; India: Manipur, Phungdhar, Iril River.G. lissorhynchusMUMF 4163–4166, 4 spec. 58.0–91.5 mm SL; India: Manipur: Tamenglong district, Noney, Iyei River.G. manipurensisMUMF 4160–4162, 3 spec. 44.0–70.0 mm SL; India: Manipur: Tamenglong district, Noney, Iyei River.G. nambulicaMUMF 8004–8008, 5 spec. 53.8–76.2 mm SL; India: Manipur: Conchak Lok stream of Nambul River.G. paralissorhynchusMUMF 5103–5106, 4 spec. 53.7–63.4 mm SL; India: Manipur: Churachandpur district, Khuga River.

2.1.2. Etymology

The species is named after its type locality: Namya River.

2.1.3. Diagnosis

A small species of Garra with smoothly rounded snout tip, prominent triangular-shaped rostral lobe with 10-11 strong, conical tubercles; proboscis and transverse groove absent; predorsal region, chest and abdomen scaled; two pairs of barbels, lateral line scales 31, circumpeduncular scales 14. Transverse-scale rows 3.5/1/3.5. Gill rakers are nine. Total number of vertebrae is 32. Dorsal fin with broad black band is near the posterior margin and a distinct W-shaped broad black band in caudal fin.

2.1.4. Description

Morphometric data of the holotype and four paratypes are given in Table 1. Body elongate, cylindrical anteriorly, gradually compressed behind dorsal fin base, body depth almost uniform. Dorsal profile of body slightly convex from tip of the supraoccipital process to dorsal fin origin. Dorsal-fin base almost straight, slightly sloping posteroventrally. Profile from posterior end of dorsal-fin base to caudal fin base slightly concave. Ventral profile of body slightly convex from pectoral to pelvic fin origin, straight from pelvic to anal-fin origin. Anal-fin base nearly straight, posterior dorsally inclined. Head moderately large, depressed with a slightly convex interorbital space; height less than its length, width greater than height. Snout broadly rounded with minute tubercles. Rostral lobe well-developed with tubercles 10(4)–11(1) in patch anterodorsal to the base of anterior barbels in about three irregular rows. Tubercles dense and pointed. Eyes placed dorsolaterally in the posterior half of head.

Table 1: Morphometric data of holotype and paratypes for Garra namyaensis sp. nov. (holotype and four paratypes).

Holotype MUMF 12O42
Range
Mean
SD

Standard length (mm)
72.5
28.1–72.5

Percentage of standard length

Body depth
19.0
19.0–21.0
20.0
0.8

Head length
22.6
20.0–25.0
24.3
1.0

Head depth
15.2
14.0–16.3
15.2
1.0

Head width
18.0
17.0–20.0
19.0
1.3

Dorsal fin length
21.0
21.0–23.1
23.1
1.8

Pectoral fin length
23.0
23.0–25.0
24.0
0.8

Pelvic fin length
19.0
18.3–20.0
19.0
0.6

Anal fin length
19.4
17.4–21.0
20.0
1.3

Length of caudal peduncle
15.0
13.0–15.2
14.0
1.0

Depth of caudal peduncle
13.0
13.0–15.0
13.4
1.1

Predorsal length
50.3
50.0–53.0
51.0
1.2

Prepectoral length
20.3
19.3–21.4
20.5
0.8

Prepelvic length
55.0
55.0–61.0
57.4
2.4

Preanal length
79.0
79.0–85.3
81.3
2.7

Percentage of head length

Head depth
67.0
54.3–83.0
66.0
10.7

Snout length
51.0
46.0–61.0
51.4
5.8

Eye diameter
15.0
15.0–25.4
19.0
4.1

Interorbital width
49.0
45.0–63.0
50.0
7.3

Disc length
34.0
34.0–44.0
39.0
4.7

Disc width
53.0
44.3–56.0
52.0
4.4

Callous pad length
23.2
21.1–34.0
25.3
5.0

Callous pad width
33.0
27.2–44.1
34.6
6.1

Percentage of head depth

Head width
118.2
118.2–126.2
122.2
3.0

Body depth
125.0
120.4–145.0
131.0
9.3

Percentage of pelvic-anal distance

Anal-Vent distance
33.1
28.0–33.3
30.3
3.8

Percentage of caudal peduncle depth

Caudal peduncle length
112.0
93.0–115.2
104
9.6

Two pairs of barbels: rostral: located anterolaterally, as long as eye diameter; maxillary: at the corner of mouth, shorter than rostral; anterior barbels short, extending to margin of rostral cap. Rostral cap well-developed; curved ventrally and connected with lower lip at the corners of mouth (Figure 1(2)). Upper jaw entirely covered by rostral cap. Lower lip modified into a mental adhesive disc. Disc elliptical, shorter than its width and narrower than head width through roots of maxillary barbels; anterior margin modified to form a transverse, fleshy, and crescentic skin fold covered by numerous tiny papillae; anteriorly separated from lower jaw by a groove running along lower jaw and posteriorly, from the central callous pad by a groove. Lateral and posterior margins of the disc surrounding central callous pad papillate and free; posteriormost margin extending a little beyond vertical from anterior margin of eye (Figure 1(3)).

Dorsal fin with ii, 6.5(5) rays, inserted anterior to pelvic fin, highest point of the body at dorsal fin origin, first branched ray longest, posterior end at same level with that of pelvic, posterior margin of dorsal fin straight. Pectoral and pelvic fins extending horizontally. Pectoral fin with i, 12(5) rays, origin close to gill opening, with round posterior margin, fourth branched ray longest end of pectoral fin not reaching pelvic fin origin, separated from it by 6-7 scales. Pelvic fin with i, 7.5(5) rays, origin situated vertically at base of second branched ray of dorsal fin rays, end of pelvic fin surpassing the anus, second and third branched ray longest separated from anal-fin origin by 2(4)–3(1) scales, posterior margin round, and length of vent-anal-fin origin about 27.5–33.3% distance of pelvic to anal-fin origin. Anal fin with ii, 4.5 rays, distal margin slightly concave, posterior end of anal-fin not reaching of caudal fin base extends to mid of caudal peduncle, distance of anal-fin origin to caudal-fin base equal to or slightly smaller than that of anal-fin origin to pelvic-fin origin, separated from pelvic fin origin by 8(4)–9(1) scales. Vent closer to anal-fin origin than to pelvic-fin origin, separated from anal-fin origin by 3(4)–4(1) scales. Caudal fin emarginated, upper lobe slightly longer than lower lobe, bearing 10 + 9(5) rays.

Lateral line complete with 31(5) scales. Scales in transverse row above lateral line and below lateral line from ventral fin origin 3.5(5) each. Circumpeduncular scale rows 14(5). Predorsal scales 13(5); scales arranged regularly, same size as flank scales. Both chest and belly scaled. Scales between vent and anal-fin base 3(3)–4(2). Gill rakers nine. Total number of vertebrae 32, consisting of 22 + 10 abdominal and caudal vertebrae.

2.1.5. Coloration

In 10% formalin, body dorsum is greyish brown, lighter posteriorly, dull greyish brown on caudal peduncle. Ventrally, head, pectoral, and pelvic fin bases are yellowish. Dark brown spot is immediately posterior to dorsal gill opening. Dorsal fin is basally yellowish white, dark black band near the posterior margin. Pectoral fin is basally yellowish with greyish submarginal band on anterior rays. Pelvic and anal-fins are greyish white. Caudal fin is with a distinct W-shaped broad black band with lines of black spots anterior to its base.

In life, body background is greenish brown, with upper angle of gill opening with shining bluish shine. Fins are golden yellow. Dorsal fin is basally yellowish with broad black band near the posterior margins. Caudal fin is with a distinct W-shaped blue black band.

2.1.6. Biology

The species inhabits swiftly flowing clear water with gravel bottom and lush green algal bloom (Figure 2). It was found adhering to rocks at the time of collection. Other species of fishes collected from the locality include species of Glyptothorax, Channa, Mastacembelus, Neolissochilus, Schistura, Tor, Puntius, and Balitora.

Figure 2: Namya River, type locality and habitat of Garra namaensis sp. nov.

2.1.7. Distribution

Garra namyaensis sp. nov. is presently known from the Namya River, Chindwin basin (Indo-Myanmar border) in Ukhrul district, Manipur state, India (Figure 3).

Figure 3: Map showing type locality of Garra namaensis sp. nov.

3. Discussion

Key to species of Garra in the Chindwin basin is given in Table 2. Among the 12 species of Garra reported by Hora [7] from the drainages of the Himalayan foothills, G. abhoyai Hora, 1921 is from the Chindwin basin. Menon [1] considered G. abhoyai as the junior synonym of G. rupecula (McClelland, 1839) which has been later revalidated and redescribed [8]. Later, G. paralissorhynchus Vishwanath and Shanta, 2005 [9] and G. nambulica Vishwanath and Joyshree, 2005 [10] have also been described from the Chindwin basin, Manipur.

Table 2: Key to Garra species of the Chindwin basin.

(1)
(i) Snout with proboscis
(2)

(ii) Snout without proboscis
(7)

(2)
(i) Proboscis well-developed
(3)

(ii) Proboscis incipient, represented by a truncate area in front of the nostrils
gravelyi

(3)
(i) Proboscis bilobed with one large
bispinosa

(ii) Proboscis unilobed or quadrate
(4)

(4)
(i) Vent to anal distance more than 45% of pelvic to anal-fin distance
elongata

(ii) Vent to anal distance less than 40% of pelvic to anal-fin distance
(5)

(5)
(i) Simple dorsal fin rays two
litanensis

(ii) Simple dorsal fin rays four
(6)

(6)
(i) Lateral line scales 34; anus to anal distance 32.1–39.2% of pelvic to anal distance
rotundinasus

(ii) Lateral line scales 36-37; anus to anal distance 22.8–28.3% of pelvic to anal distance
qiaojiensis

(7)
(i) Caudal fin with W-shaped black mark
(8)

(ii) Caudal fin without W-shaped black mark
(9)

(8)
(i) Caudal fin forked
nambulica

(ii) Caudal fin slightly emarginated
(10)

(9)
(i) Rostral lobe present
poecilura

(ii) Rostral lobe absent
(11)

(10)
(i) Snout without tubercles
abhoyai

(ii) Snout with tubercles
(14)

(11)
(i) Snout without barbels
imberbis

(ii) Snout with barbels
(12)

(12)
(i) Lateral line scales 30-31
spilota

(ii) Lateral line scales more than 37-38
(13)

(13)
(i) Simple dorsal fin rays two; depth of body 17.0–17.6% SL
compressus

(ii) Simple dorsal fin rays four; depth of body 20.4–24.6% SL
tengchongensis

(14)
(i) Rostral lobe absent, chest, and abdomen naked
Paralissorhynchus

(ii) Rostral lobe present, chest, and abdomen scaled
namyaensis

The new species under description shares similar characters with G. abhoyai, G. nambulica, and G. paralissorhynchus occurring in the Chindwin basin in having a dark black band on the posterior margin of the dorsal fin and a broad black W-shaped band on the caudal fin. Menon [1] grouped these fishes under Lissorhynchus complex based on the colour markings mentioned above. However, G. namyaensis sp. nov. is easily distinguished from the three congeners in the presence (versus absence) of a rostral lobe on the snout. It further differs from G. abhoyai in having 12 (versus 13) branched pectoral fin rays, scales on the chest and abdomen (versus naked), longer dorsal fin (21.0–22.2% SL versus 14.2–11.5), and pectoral fin (22.5–24.7% SL versus 20.0–21.5); from G. nambulica in having 31 (versus 34-35) scales on the lateral line, a longer snout (49.0–61.0% HL versus 28.9–32.9), a shorter disc (30.5–44.0% HL versus 57.2–64.5), and scales on the chest and abdomen (versus naked); and from G. paralissorhynchus in having 12 (versus 11) branched pectoral fin rays, 19 (versus 17) caudal fin rays, 13 (versus 11-12) predorsal scales, and scales on the chest and abdomen (versus naked).

Of the seven species of Garra described from the Rakhine Yoma, Southern Myanmar by Kullander and Fang [2], two species, namely, G. flavatra Kullander and Fang, 2004 and G. poecilura Kullander and Fang, 2004, resemble G. namyaensis sp. nov. in having distinct coloured markings on the caudal fin. However, the new species is easily distinguished from G. flavatra in having a distinct W-shaped band (versus two vertical bars of irregular black spots) on the caudal fin, two (versus three) each of branched dorsal and anal-fin rays, 31 (versus 28) lateral line scales and 13 (versus 10) predorsal scales. G. namyaensis sp. nov, can also be distinguished from G. poecilura in having a distinct W-shaped black band (versus two vertical rows of black spots) across the middle of caudal fin, two (versus three) unbranched dorsal fin rays, and a shorter (19.7–24.9% SL versus 26.0–27.2) and narrower (18.0–20.0% SL versus 21.7–22.5) head.

The presence of a prominent triangular-shaped rostral lobe on the snout easily distinguishes Garra namyaensis sp. nov from all its congeners except G. poecilura of the Chindwin-Irrawaddy basin and G. rakhinica Kullander and Fang, 2004, G. propulvinus Kullander and Fang, 2004, G. flavatra, G. vittatula Kullander and Fang, 2004, and G. nigricollis Kullander and Fang, 2004 of the rivers draining the western face of the Rakhine Yoma. The new species can be easily distinguished from G. flavatra and G. poecilura in the differences of the caudal markings as mentioned above and from the rest in the presence (versus absence) of a W-shaped band on the caudal fin. The new species further differs from G. poecilura in having tubercles scattered both on the snout and the rostral lobe (versus tubercles restricted to rostral lobe only) and 31 (versus 29) lateral line scales; from G. propulvinus in having two (versus three) unbranched dorsal fin rays, 12 (versus 13) branched pectoral fin rays and 13 (versus 10) predorsal scales; from G. rakhinica in having two (versus three) unbranched dorsal fin rays, a shorter dorsal fin (21.0–22.2% SL versus 28.7–32.3) and 31 (versus 28) lateral line scales; from G. nigricollis in having 31 (versus 33) lateral line scales, 12 (versus 15) branched pectoral fin rays, a shorter dorsal fin (21.0–22.2% SL versus 28.4–29.8), and 13 (versus 11) predorsal scales; from G. vittatula in having a shorter caudal peduncle (13.3–15.2% SL versus 16.0–19.1), two (versus three) unbranched dorsal fin rays, and 12 (versus 14) branched pectoral fin rays; from G. flavatra in having narrower head (18.0–20.0% SL versus 21.4–24.7), a shorter pelvic fin (18.3–20.0% SL versus 22.2–24.7), and 31 (versus 28) lateral line scales.

A thorough revision of the species of Garra is required since no types have been designated for many of the described species, and also there are taxonomic ambiguities of some nominal species. In addition, molecular studies would clarify the species status of the described species and also help in understanding the phylogenetic relationship among the species.

Acknowledgments

B. Shangningam is grateful to University Grants Commission (UGC) for providing University Research Scholarship, and W. Vishwanath is grateful to the Department of Science and Technology, Government of India (Project no. SR/SO/AS-50/2008).

References

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