Marine Benthic Algae of the Commander Islands (Pacific Coast of Russia) with Checklist Revised in 2012

A revised checklist of marine benthic algae of the Commander Islands (Russian Pacific) is presented. It is based on the authors’ field collections. The list of algae of the area totals to 187 species (36 Chlorophyta, 38 Ochrophyta, class Phaeophyceae, and 113 Rhodophyta) of these 44 species (9 Chlorophyta, 8 Ochrophyta, class Phaeophyceae, and 27 Rhodophyta) are added to the previous checklists (Selivanova and Zhigadlova, 1997).We also confirm the presence of 5 species on the Islands that were absent in our earlier material but were recorded by other authors (Phycodrys amchitkensis, Mastocarpus papillatus, Lithothamnion sonderi, Odonthalia dentata, and Pleonosporium vancouverianum). The species list of the newly recorded algae contains information on their ecology, fertility, and distribution. The algal taxonomy and nomenclature are updated with new world data.


Introduction
Information on the marine algae of the Commander Islands is more comprehensive than in other regions of the Russian Pacific.The Islands' flora has been studied more thoroughly and described in many publications by Russian and foreign authors [1][2][3][4] including our works [5][6][7], where we presented preliminary results of the revision of the marine algal flora.Further studies were presented in our publications [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24], but the majority of these papers were published in Russian, some of them by local publishing houses, so they are not readily available to phycologists outside of Russia.However, the marine algal flora of the Commander Islands is of more than local interest because at this archipelago the Bering Sea meets the open Pacific Ocean between the Asian and American continents, and so the Islands act as a bridge for species intercontinental migration and exchange due to the system of ocean currents (Figure 1).
The inventory of the flora of this area is still unfinished because of the difficulty of collecting algae in this remote and sparsely inhabited region.Furthermore, there are still unsolved nomenclatural problems.Therefore, main purpose of this study was to incorporate our new collections and update the nomenclature and taxonomy of algae to further advance knowledge of the flora of the Commander Islands.

Materials and Methods
The material was collected by the authors during annual expeditions of the Hydrobiology Laboratory of the Kamchatka Branch of Pacific Geographical Institute in 1986-1992 and incidental collections in 1997 and 2007-2011.Algae were collected from April through October on the littoral fringe during low tides, and with use of SCUBA at depths of 1-30 m.Algae cast ashore were also collected.Other individual collectors also took part in this work.Particularly interesting algal material used in this study was collected by our colleague zoologist Nikolai N. Pavlov on Bering Island in winter season of 2011.
Material was sectioned freehand with a razor blade, placed in a drop of fresh water on a slide, and examined with a light microscope.The sections were studied unstained, or stained with Lugol's iodine solution and aniline blue dye.The processing of collections was conducted at the Kamchatka Branch of the Pacific Geographical Institute (Petropavlovsk-Kamchatskii, Russia).Photomicrographs were made using

Results
The present paper summarizes new information on the marine macrophytic algae of the Commander Islands that has appeared since our previous revision of the flora 15 years ago [5][6][7].
The new general checklist of the marine benthic algae of the Commander Islands is provided in Tables 1-3 (Annex) in the Supplementary Material available online at http://dx.doi.org/10.5402/2013/470185.Outdated or erroneous names of the species from our previous publications are cited in the tables, and comments are provided on the current taxonomic position of algae.
At present, the marine flora of the Commander Islands is known to contain 187 species (36 Chlorophyta; 38 Ochrophyta, class Phaeophyceae and 113 Rhodophyta), 44 are new records since our previous list (see: Selivanova and Zhigadlova [5][6][7]).The former checklist contained 149 species,  however later several species had been synonymized and the number of algae decreased.
Information on 44 added species is given below, including data on the ecology, fertility, and distribution of the algae on the Islands.Most recent findings are depicted on the figures.
Taxonomic notes are made where pertinent.Numbers in parentheses following the names of the islands of the archipelago in the species list correspond to collecting sites shown on the map (Figure 1).2).Found in August, very rare, epiphytic on Palmaria stenogona, intertidal.Bering Island (2).With structures presumably representing sporangia.
Note.Geographic range of this species remains controversial.P. purpurea was considered to have both Pacific and Atlantic distribution [27][28][29][30][31][32].Later it was revised by Lindstrom and Cole [33,34], who showed by biochemical and genetic analyses that true P. purpurea was distributed only in the Atlantic Ocean, while its Pacific vicariant was described by these authors as a new species, Porphyra kurogii S. C. Lindstrom [34].The morphological description of P. kurogii, however, was considerably different from that of P. purpurea given by Perestenko [29,30,35], who insisted on wide Atlantic-Pacific distribution of P. purpurea.According to Silva et al. [36] P. purpurea is also met in Indian Ocean.Later on morphological and molecular studies on Atlantic P. purpurea complex were carried out by western phycologists [26,37,38].As a result of these studies a considerable part of the species of the former genus Porphyra were transferred to the genus Pyropia [26] but P. purpurea was retained within the genus Porphyra as a type species [26].There were specimens of Porphyra in our material that morphologically represented Porphyra purpurea in Perestenko's [35] interpretation.There were also specimens that fit into former Porphyra kurogii that is now transferred to the genus Pyropia as Pyropia kurogii (S. C. Lindstrom) S. C. Lindstrom [26].Both Porphyra purpurea and Pyropia kurogii are presented in Supplementary Table 3.
Note.Gloiocladia guiryi appears to be the first and up to now the only representative of the genus and the family Faucheaceae to be reported from the Far-Eastern Seas of Russia.Originally it was described as Fauchea guiryi Selivanova [14].However, a taxonomic revision of the genus Fauchea Bory and Montagne in Montagne based on morphological and molecular-genetic data resulted in the transfer of the majority of the species of the genus, including the generitype F. repens (C.Agardh) Montagne and Bory de Saint-Vincent, to the genus Gloiocladia J. Agardh [39].This necessitated the transfer of F. guiryi to Gloiocladia [15] with a new nomenclatural combination, Gloiocladia guiryi (Selivanova) Selivanova [16].
Note.Some specimens of this species were erroneously identified previously as Palmaria marginicrassa.Note.Some samples of this species were erroneously identified previously as Palmaria stenogona.
Note.This species was previously attributed to the family Petrocelidaceae on the basis of the presence in its life history a stage of development known as Petrocelis Kützing.Later it was shown to belong to the Phyllophoraceae on the basis of molecular-genetic studies by Fredericq and Ramirez [41].
Note.This species was for a long time treated in Russian taxonomic literature as Neorhodomela larix (Turner) Masuda subsp.aculeata Perestenko.As N. aculeata it was recorded by Klochkova et al., [42] from the near-Kamchatka water areas, however the Commander Islands were not mentioned by these authors.So this is a new record of N. aculeata precisely from the Commander Islands.

Discussion
Although the present paper increases the checklist of the marine algae of the Commander Islands from 149 to 187 species, the list is still incomplete and will continue to grow as more collections are processed.In this paper, we recorded 44 species found after 1997.The examined specimens of the new additions are in good agreement with previous descriptions of the taxa [28-30, 35, 43-57].
In addition, we found 3 macrophytes: brown alga Scytosiphon dotyi and 2 red algae: Neorhodomela aculeata and Pyropia nereocystis that are newly recorded from the Commander Islands.The latter also appears to be new to the Russian Pacific region.
We included Gloiocladia guiryi described from the Commander Islands [14][15][16] as a new record in our list for the reason that our revised checklist summarizes all available information appeared after the first revision of the Islands' flora [5][6][7].
We also confirm the presence of 5 species that were recorded by other authors but were absent in our earlier collections.Phycodrys amchitkensis was recorded as presumably inhabiting Commander Islands by Gusarova and Semkin [58], and our collections of this alga on Medny Island confirm their supposition; Mastocarpus papillatus was recorded by Perestenko [59]; Lithothamnion sonderi, Odonthalia denand Pleonosporium vancouverianum were recorded by Klochkova and Berezovskaya [60].
On the whole the marine algal flora of the Commander Islands can be characterized as diverse in its species composition and allochthonous in origin.No endemic species were found.The diverse species composition shows that the floristic complex of the Commander Islands is under the strong influence of adjacent areas.
The main ecological factor shaping the contemporary flora of algae macrophytes in the region is the system of marine currents.The Commander Islands are greatly influenced by the American continent due to a branch of the Alaska current (Figure 1) providing penetration of American elements into the Islands' flora.We believe this process is the main influence on the development of the flora of the Commander Islands, providing greater richness and diversity compared to other areas of the Bering Sea.Many species found by us for the first time on the Islands from 1986 to 1997 were earlier described from the American coast (e.g., Palmaria callophylloides M. W. Hawkes & Scagel, P. hecatensis, Laingia aleutica M. J. Wynne, Beringiella labiosa M. J. Wynne, and Tayloriella abyssalis M. J. Wynne).Our present study confirms this tendency, as the species we found there in the last decade were previously described from the American coast as well (Ulvella prostrata, Acrochaete apiculata, A. geniculata, A. ramosa, Hecatonema primarium, Streblonema evagatum, S. myrionematoides, S. scabiosum, Compsonema serpens, Scytosiphon dotyi, Acrochaetium arcuatum, A. densum, A. porphyrae, Colaconema desmarestiae, C. pacificum, Pyropia nereocystis, etc.).Most of them are not known up to now from the other Russian Pacific regions.Of course, the absence of records of some species from the Russian sector of the North Pacific does not in all cases mean that these algae do not grow there.Probably it is caused by less careful studies in this area in contrast to the Commander Islands.Moreover, it should be noted that in spite of the fact that all above-mentioned species were described from the American continent, they are not necessarily American in their origin.It is quite possible that marine algal flora of the American continent is more comprehensively studied and new species are found and described there more often in comparison with the Russian Pacific sector.
In any case, according to our data (Supplementary Tables 1-3), the presence of 23 species (about 12% of the total number in our checklist) on the Commander Islands that are not met in other areas of the Russian Pacific but are common with the American coast proves our supposition that the marine flora of the Commanders is appreciably influenced by the American floristic elements.In addition, it is possible to indicate some algal species occurring on the Commander Islands that were originally described from the American sector but now found also on the Pacific coasts of Russia: Scytosiphon dotyi, Palmaria callophylloides, P. hecatensis, P. mollis, Constantinea subulifera Setchell, Neodilsea natashae S. C. Lindstrom, Phycodrys amchitkensis, and Tokidadendron bullatum (N.L. Gardner) M. J. Wynne.Presumably these species migrated from American to Asian continent via the Commander Islands.So the Islands serve as a peculiar bridge uniting American and Asian algal floras.Certainly the Aleutian Islands form the major part of this bridge.Considerable similarity of the floristic and faunistic complexes of the Commander and Aleutian Islands was noted by Kussakin and Ivanova [4].Obviously it is due to similarity in geographic position, geological structure of both archipelagoes, and hydrological conditions providing continuous species exchange between the continents.According to our calculations there are 165 species of algae growing on the Commander Islands that also occur in the American sector of the North Pacific.At the same time there are 165 species of the Commander Islands' flora common with the other areas of the Russian Pacific sector.So the Commander Islands' marine flora has equal similarity with Asian and American floristic complexes.There are 140 species included in our list that are common for all discussed areas.

Conclusion
The Commander Islands marine algal flora is species rich.Marine macrophytes are among the most dominant components of benthic communities on the shelf, so an inventory of the algae is very important.The new checklist will provide important background material for studies on biodiversity, ecology and phytogeography.Knowledge of the contemporary algal flora will also be helpful for interpreting of historical phytogeography, and the processes of species formation and migration.Increased knowledge of the marine flora of the Commander Islands and of other areas of the Russian Pacific coasts assures a better understanding of the occurrence and functioning of benthic communities as a whole.Inventory of the marine flora is necessary not only for clarification of scientific questions but also for solving of some important practical problems.In particular, there is a threat of biodiversity decrease in the region and the loss of still unknown species.Already known rare species included in the Red Data Book of Kamchatka are also under the threat of loss.It is essential to continue studies on biodiversity of marine coastal ecosystems paying special attention to their plant components that provide habitat and/or food for valuable commercial species (herring, rock trout, sea urchins, etc.).These studies help to fulfill the task of biodiversity conservation and sustainable nature use in Kamchatka region.

Figure 3 :
Figure 3: Acrochaete geniculata.Surface view of the filaments among cells of the host plant.

20 𝜇mFigure 5 :
Figure 5: Acrochaete repens.Filaments with hairs in the tissues of the host plant.

Figure 16 :
Figure 16: Acrochaetium densum basal portion of the plant showing original two cells (arrow).

Figure 20 :
Figure 20: Colaconema endophyticum.Filaments of the epiphyte among the cells of the host plant with monosporangia (arrows).

Figure 21 :
Figure 21: Meiodiscus concrescens.Surface view of the epiphyte on the upper part of the figure.Cross-section showing tetraspores (arrows) on the lower part of the figure.