Diagnostic of pathogen in the human biological liquids by biochip technology is an intensively developed methodic now. The main and the most important part of biochip is the adsorbing layer. Adsorption properties of chalcogenide films to protein (rat monoclonal antibodies) were tested. The films were prepared by conventional thermal deposition technique and by pulsed laser deposition technique. Two methods were used for forming in the films the two-dimensional map of adsorbing places for probe testing. One is using photoresist properties of chalcogenide films; another is using photo-induced oxidation of chalcogenide films. It was shown Good selectivity of the developed structures to protein markers was shown.
Diagnostic of pathogen in the human biological liquids by biochip technology is an intensively developed methodic now. The most important part of biochip is the adsorbing layer.
There are two main problems in developing this layer. One is creation of pattern on the film-surface and the second is achievement of selectivity of adsorption of protein or DNA to the created pattern [
Methods of patterning and adsorption properties of chalcogenide films to protein or DNA were investigated in this work for the biochip application.
The glasses of As39S61 and Ge26Ga9S65 compositions were synthesized in evacuated quartz ampoules from elementary arsenic and sulfur or elementary germanium, gallium, and sulfur. The prepared As39S61 glass was used to deposition of thin films onto standard glass slide by thermal deposition. The film thickness was about 500 nm. The synthesized Ge26Ga9S65 glass was used for preparation of the thin films by pulsed laser deposition (XeCl laser was used). Pulsed laser deposition from one rotating target (pies of glass) leaded to formation of film with nonuniform surface geometry. Therefore, the special target consisting of 12 glass pieces arranged in a circle at different angles to the laser beam was used. During laser ablation, the target revolved permanently, so the plasma stream changed permanently its angle relative to the substrate, increasing the uniformity of the surface of the deposited film [
The mercury lamp was used for UV irradiation of the deposited films. For test of the adsorbing ability, the following protein and DNA materials marked with fluorescent labels were used: green recombinant protein (EGFP) (1 : 20 and 1 : 200 dilution); pure hemagglutinin derived from the influenza virus type A, conjugated to a fluorescent label Cy3 (HA-Cy3) (1 : 10 and 1 : 100); mouse antibody to rabbit immunoglobulins conjugated to a fluorescent label Cy5 (RAM-Cy5) (1 : 100 and 1 : 1000); mouse antibody to rabbit immunoglobulins conjugated to a fluorescent label Cy3 (RAM-Cy3) (1 : 100 and 1 : 1000); labeled amplicon prepared by PCR with the nucleotide dCTP-Cy5 (DNA-Cy5) (1 : 100 and 1 : 1000); short probe with NH2 group at the 5′-end and marker Cy3 at the 3′-end (NNMolig-Cy3) (1 : 1000 and 1 : 10000); short probe NH2 group at the 5′-end and marker Cy5 at the 3′-end (NNMolig-Cy5) (1 : 1000 and 1 : 10000).
The kinetic of photostructural transformation was studied to optimize the irradiation conditions of the films. Thus, the changing of the fundamental absorption edge position of the film after irradiation with UV light was investigated. The optical absorption spectra of the source film and the irradiated films for 3, 5, and 7 minutes are shown in Figures
(a) and (b) The absorption spectra of As39S61 films. The source film and the irradiated films for 3, 5, and 7 minutes. (c) The dependence of the shift of the fundamental absorption edge of As39S61 film on exposition time.
According to the dependence shown in Figure
The Raman spectra of the As39S61 film, source film, and the irradiated film for 7 minutes.
Decreasing intensity of the peaks at wavenumbers 364, 345, 223, and 191 cm−1 (which belong to As4S4 structure units testifies decreasing concentration of these structure units) relatively the basic structural units AsS3/2 [
The above-mentioned results were used in order to create the test microarray. The deposited As39S61 film was irradiated with UV light through a mask (opaque shield with 16 subarrays that consist of 9 holes (array
After that the prepared substrates (glass slide with chalcogenide glass islands) were washed 15 min in a solution of 1x PBS-T (concentration of Tween 20 was 0.05%) and scanned at wavelengths of fluorescence of the three markers—Cy3 (582 nm), Cy5 (664 nm) and GFP (509 nm). All dilutions of the protein samples were prepared in 1x PBS. DNA samples were diluted in Milli-Q water. All 16 subarrays were divided with special frames. 100 mcl of a protein or DNA solution was applied to each subarray on the substrate. Then it was treated for 1 hour at 37°C on shaker PST-60 HL Plus (Biosan). After that, it was washed from unbound molecules 2 times for 5 min in PBS-T; then frames were removed and the whole substrate was washed for 5 minutes in Milli-Q water. The prepared substrate was scanned on a fluorescent scanner Scan Array Express (“PerkinElmer”, USA) with the following parameters: PMT 70, RES 20. The excitation and scanning wavelengths depended on the used fluorescent marker.
For example, fluorescent images of subarrays of chalcogenide glass islands onto oxide glass slide treated with RAM-Cy5 and HA-Cy3 are shown in Figure
Fluorescent image of subarrays with chalcogenide glass film islands treated with RAM-Cy5, HA-Cy3, and NNMolig-Cy5.
The kinetic of photooxidation of the deposited Ge26Ga9S65 films was studied with optical absorption spectroscopy. Irradiation of the deposited film with mercury lamp for 20 min results in saturation of the photooxidation effect. Figure
The absorption spectra of the glass slide, source Ge26Ga9S65 film, and the film irradiated for 20 minutes.
Adsorption ability of the irradiated and nonirradiated areas of the films to protein and DNA was investigated. The first preliminary results were obtained. The ability of the prepared Ge26Ga9S65 film to adsorb protein molecules was evaluated using microarray technology. Proteins marked with fluorescent markers (RAM-Cy5, HA Cy3, and GFP) in concentration 1 : 100 were printed on the film. Then film was scanned with fluorescent scanner ScanArray Express. After that, film was washed for 5 minutes in a buffer solution 1xPBS with stirring and then film was scanned again. As result, all the tested proteins were adsorbed on the chalcogenide films. For example, fluorescent images of the film with printed RAM-Cy5 before and after washing treatment are shown in Figure
Fluorescent image of RAM-Cy5 deposited onto Ge26Ga9S65 chalcogenide film. Left image is film with deposited RAM-Cy5; right image is the same film washed after that.
In this way we created the test methods of patterning from chalcogenide glassy thin film and demonstrated the presence of selective adsorption of protein markers to such structures. The obtained data shows the perspective for the use of chalcogenide films as substrates for biochips with spatially defined selective adsorption of biological probes.
The authors declare that there is no conflict of interests regarding the publication of this paper.
This work was supported by Grant of St. Petersburg State University no. 12.39.1047.2012.