miR-486 Promotes the Invasion and Cell Cycle Progression of Ovarian Cancer Cells by Targeting CADM1

Objective To explore the role and possible underlying mechanism of miR-486 in ovarian cancer (OC) cells. Methods The expression of miR-486 and CADM1 was detected by qRT-PCR in OC tissues and adjacent nontumor tissues and OC cell lines. The dual-luciferase reporter gene system was used to determine the targeting relationship between miR-486 and CADM1. CCK-8, colony formation assay, Transwell, and flow cytometry were performed to detect cell proliferation, cell invasion, cell cycle progression, and the apoptotic cell death, respectively. Western blot was carried out to detect the expression of CADM1 protein and the proteins associated with cell cycle progression. Results miR-486 was significantly upregulated in OC tissues and cells, while CADM1 expression was significantly downregulated. Dual-luciferase reporter assays further confirmed that CADM1 was a target gene of miR-486. Interference with miR-486 could inhibit the proliferation and invasion and promoted the apoptosis of SKOV3 cells. Knocking down both miR-486 and CADM1 significantly increased the SKOV3 cell proliferation, invasion, and the number of cells transitioning from the G0/G1 phase into the S phase of cell cycle and reduced the cellular apoptosis. Western blot analysis revealed that the expression of cell cycle progression-related proteins (CyclinD1, CyclinE, and CDK6) was significantly reduced, and the p21 expression was increased when interfering with both miR-486 and CADM1 expression. Conclusion Our results suggested that miR-486 could act as a tumor promoter by targeting CADM1 and be a potential therapeutic target for the treatment of OC.


Introduction
Ovarian cancer (OC) ranks the third among the most common malignancies of the female reproductive system, with the highest mortality rate among malignant gynecological tumors [1]. Although the combined treatment of multiple disciplines such as surgery, chemoradiotherapy, and molecular target therapy is currently used in clinical practice, the incidence and mortality of OC remain high in China [2,3]. OC has no significant early symptoms, no reliable tumor biological markers, and lack of effective therapeutic targets but is easy to recur, so its treatment has entered a bottleneck [4]. Local and systemic metastasis is the main cause of higher mortality in OC patients [5], but the specific mechanism of metastasis has not been clarified. A total of 92% histological type of OC is epithelial OC, of which high-grade serous ovarian cancer (HGSOC) accounts for more than two-thirds of reported cases [6]. Despite the different pathological types of tumors, surgical treatment combined with chemotherapy and other combined treatments are commonly applied, with no significant efficacy. OC still poses a serious threat to women's life and health. Therefore, in order to improve the prognosis of OC patients, it is essential to explore the potential mechanisms of tumor metastasis and identify new therapeutic targets.
MicroRNAs (miRNAs) are endogenous small noncoding RNAs with about 19 to 25 nucleotides in length. miRNAs can negatively regulate target gene mRNA expression and reduce the stability of mRNAs by binding to the 3 ′ -untranslated region (3 ′ -UTR) of target gene mRNA [7]. Despite their increasingly reported crucial roles in the mammalian genome [8], the dysregulation of miRNAs is closely related to the initiation and development of various types of tumors [9], including ovarian cancer [10]. For instance, miR-137 is downregulated in OC and forced expression of miR-137 can promote apoptosis of OC cells by inhibiting the expression of XIAP [11]; miR-125b slows OC progression by targeting SET protein to inhibit the epithelial-mesenchymal transition pathway [12]; miR-365 inhibits OC progression by targeting Wnt5a [13]; miR-18a inhibits OC growth by directly targeting TRIAP1 and IPMK [14]. Thus, miRNAs are closely related to the development of OC.
Recently, abnormal expression of miR-486 has been found in many related studies of tumors, which may be associated with tumor development. miR-486 is upregulated in OC, and some studies have found a close relationship between miR-486 and OC [15,16]. Therefore, miR-486 may play an important role in the development and progression of OC. However, its detailed function and molecular mechanism in OC are still unclear and need to be further explored. Therefore, to determine the expression and biological function of miR-486 expression in OC, we first validated miR-486 expression in OC tissues. Subsequently, the effect of miR-486 expression on OC cell function and molecular mechanisms was examined by cellular assays. This study will provide a more comprehensive theoretical basis for miR-486 as a therapeutic target for OC. were used. All the cells were cultured in RPMI-1640 culture medium supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin and incubated at 37°C with 5% CO 2 and saturated humidity. The cells were digested with 0.25% trypsin every 2-3 days for passage, and the cells in the logarithmic phase were used for subsequent experiments.

Materials and Methods
2.3. Cell Transfection. miR-486 inhibitor (in-miR-486), Normal Control inhibitor (in-NC), siRNA NC (si-NC), and CADM1 siRNA (si-CADM1) were designed and synthesized by RiboBio (Guangzhou, China) and used in accordance with the manufacturer's instruction. SKOV3 cells were divided into five groups: in-miR-486 group, in-NC group, in-NC +si-NC group, in-miR-486+si-NC group, and in-miR-486 +si-CADM1 group. SKOV3 cells were inoculated in 6-well plates at a density of 5 × 10 5 cells/well to 50%-70% confluency. Then, they were transfected using Lipofecta-mine2000 (Invitrogen, USA) for 6 hours. After the transfection, the cells were rinsed three times with PBS, the culture medium was replaced with common culture medium, and then, culture continued for a further 48 hours. The transfection efficiency was verified using qRT-PCR, and the cells with successful transfection were used for subsequent experiments.

Cell Counting
Kit-8 (CCK-8) Assay. The cells in the logarithmic growth phase were digested into single cell suspension and inoculated in 96-well plates at a density of 5 × 10 3 cells/well. At 0, 24, 48, and 72 hours after transfection, the cell growth status was measured using CCK-8 (Beyotime Biotechnology, China). The plates were incubated for 1 hour added with 10 μl of CCK-8 solution. Subsequently, the absorbance values were detected at 450 nm using a microplate reader (Bio-Rad Laboratory).

Colony Formation Assay.
At 48 hours after transfection, SKOV3 cells in 6-well plates were digested with 0.25% trypsin and centrifuged at 1000 r/min for 5 min to resuspend and count. The cell suspension with gradient dilution was inoculated in the culture dish at appropriate cell density and the cells in even distribution at the bottom as possible. Then, they were cultured for 10 days until the colony is visible to the naked eye. The original culture medium was removed. The cells were rinsed with PBS once, added 10% formaldehyde for fixation for 15 min, rinsed with PBS twice, stained with crystal violet for 30 min, rinsed with PBS, dried, and finally photographed.
2.6. Transwell Assay. A total of 50 μl serum-free medium was used to wet the polycarbonate membrane. The cells at 48 h after transfection were rinsed three times with serum-free medium. Then, 1 × 10 5 cells/well were inoculated into the upper chamber with 100 μl medium while 500 μl complete medium was added to the lower chamber as chemokines for 12 h incubation. The culture was terminated when 5 cells passed to the lower chamber, and the polycarbonate membrane was removed. The invasive cells were rinsed with PBS three times, fixed in neutral formaldehyde for 10 min, and stained with hematoxylin. Their number was counted under a microscope in random five fields, and the mean value represented the cell invasion ability. Analytical Cellular Pathology centrifuged at 1000 r/min for 5 min. The supernatant was aspirated, and then, 195 μl of Annexin V-FITC binding solution was added to resuspend the cells. Subsequently, 5 μl of Annexin V-FITC reagent was added, mixed gently, and placed at room temperature for 10 min in the dark. Another 10 μl of propidium iodide (PI) staining solution was added and placed at room temperature for 10 min in the dark. Then, the cells were resuspended by adding 200 μl of Annexin V-FITC binding solution. Apoptosis was detected using flow cytometry (FACSCanto TM II, BD Biosciences). In addition, 1 × 10 6 cells were rinsed with PBS, fixed in precooled ethanol overnight at 4°C, and stained with PI after treatment with RNaseA (Sigma, USA). Finally, the cell cycle was analyzed by flow cytometry.
2.8. Dual-Luciferase Reporter Assay. Cell adhesion molecule 1 (CADM1) was predicted to be a target gene of miR-486 using Starbase V2.0, and their binding was verified by the luciferase reporter. The 3 ′ -UTR of the wild-type (WT) CADM1 gene was cloned into the luciferase plasmid, and the mutant-type (MUT) plasmid was available from the mutation of CADM1 and miR-486 binding domain. SKOV3 cells were inoculated in 24-well plates to 60% confluency. The cells were transfected using Lipofectamine2000 according to the following groups: miR-486 mimics and WT plasmid containing CADM1 3′-UTR; NC mimics and WT plasmid containing CADM1 3′-UTR; miR-486 mimics and MUT plasmid containing CADM1 3′-UTR; and NC mimics and MUT plasmid containing CADM1 3′-UTR. After 48 hours of cotransfection, the relative luciferase activity levels of the different groups were measured using a dual-luciferase reporter assay system.
2.11. Statistical Analysis. All experiments were repeated three times, and the experimental data were expressed as mean ± standard deviation (SD). SPSS22.0 software was used for statistical analysis. One-way analysis of variance (ANOVA) followed by Tukey's post hoc test was applied for the comparison of multiple groups, while t-test was applied for the comparison between two groups. P < 0:05 indicated a statistically significant test result.

miR-486 Is Highly Expressed in OC Tissues and Cells.
To confirm the aberrant expression of miR-486 in the OC tissue, we assessed the relative expression in comparison with normal counterparts using qRT-PCR. The results confirmed that miR-486 expression was significantly increased in OC tissues compared with that in normal tissues (Figure 1(a)). In addition, miR-486 expression was also detected in SKOV3, Caov3, ES-2, OVCAR3, and IOSE80. However, as shown in Figure 1(b), miR-486 expression was significantly increased in SKOV3, Caov3, ES-2, and OVCAR3 compared with that in the normal IOSE80 cells. These results showed that miR-486 may be involved in the development of OC. Since SKOV3 cell line showed the highest endogenous expression of miR-486, they were selected for subsequent experiments. Figure 1(c) shows the successful knockdown of miR-486 using miR-486 inhibitor.

3.2.
Interfering with miR-486 Expression Inhibits the Biological Function of OC Cells. To investigate the biological function of miR-486 in OC cells, after exogenously manipulating the miR-486 expression, CCK-8, colony formation assay, Transwell assay, and FCM were used to detect cell proliferation, invasion, and apoptosis, respectively. The results showed that in comparison with the in-NC group, cell viability and proliferation (Figures 2(a) and 2(b)) and invasion (Figure 2(c)) in the in-miR-486 group were significantly reduced. Flow cytometry analysis showed a significant increase in the apoptotic death in-miR486 group (Figure 2(d)) and an increased proportion of cells in the 3 Analytical Cellular Pathology G0/G1 phase of the cell cycle with a concurrent reduction of cells in the S phase (Figure 2(e)). Western blot was used to detect the expression levels of proteins associated with cycle progression. The result showed that the protein expression of CyclinD1, CyclinE, and CDK6 was significantly decreased and p21 was significantly increased in the in-miR-486 group compared with the in-NC group (Figure 2(f)). The above results suggest that interference with miR-486 expression inhibited the biological function of OC cells.
3.3. CADM1 Is a Target of miR-486 in OC. miRNAs have been widely reported to repress the expression of target genes by binding to 3′-UTR [7]. Hence, we tried to find the targets regulated by miR-486. According to the prediction by Starbase V2.0 database, CADM1 could be one of the target genes of miR-486 (Figure 3(a)). To verify the targeting relationship between miR-486 and CADM1, WT or MUT containing CADM1 3 ′ -UTR and miR-486 mimics or mimic NC were cotransfected in SKOV3 cells to detect luciferase activity. As shown in Figure 3(b), luciferase activity was significantly inhibited after cotransfection with miR-486 mimics and the WT CADM1 3′-UTR. The results of qRT-PCR and Western blot showed that mRNA and protein expression levels of CADM1 were significantly decreased in OC tissues and cells (Figures 3(c)-3(e)). Subsequently, we found that knocking down miR-486 significantly upregulated the mRNA and protein expression of CADM1 in SKOV3 cells (Figures 3(f) and  3(g)). The correlation analysis revealed that there was a negative correlation between miR-486 and CADM1 expression (Figure 3(h)). These results suggested that miR-486 regulated the expression level of CADM1 in OC.     Analytical Cellular Pathology   Analytical Cellular Pathology 486 exerts its biological function in OC by regulating CADM1, the rescue experiment was carried out by transfecting in-miR-486, in-NC, si-NC, si-CADM1, and in-miR-486 +si-CADM1 in SKOV3 cells. The results showed that cell proliferation, viability, and invasion were significantly reduced, apoptosis was significantly increased, and the proportion of cells in the G0/G1 phase was significantly increased, while in the S phase, it was significantly reduced in the in-miR-486 group (P < 0:05). However, knocking down CADM1 in the same group reversed the miR-486 inhibition effects on different OC hallmarks (Figures 4(a)-4(e)). In addition, as shown in Figure 4(f), compared with the in-NC+si-NC group, the protein expression levels of CyclinD1, CyclinE, and CDK6 were significantly decreased while the protein expression levels of CADM1 and p21 were significantly increased in the in-miR-486+si-NC group; compared with the in-miR-486+si-NC group, the protein expression levels of CyclinD1, CyclinE, and CDK6 were significantly increased while the protein expression levels of CADM1 and p21 were significantly decreased in the in-miR-486+si-CADM1 group (P < 0:05). These results indicated that miR-486 downregulates CADM1 expression to affect the biological function of SKOV3 cells.

Discussion
OC is a gynecological malignant tumor with the highest mortality rate among the three malignant tumors of the female reproductive system [1,17]. With medical advances, the prognosis of OC patients has improved, but the 5-year survival rate is still low due to recurrence, drug resistance, early metastasis, and lack of effective targeted therapy [18]. Therefore, exploring the mechanism of OC hallmarks is essential for the new treatment strategies. In recent years, miRNAs have attracted attention of many scholars because of its importance in the early diagnosis, occurrence and development of tumors, and tumor cell invasion and migration [19,20].
Previous studies have found that miR-486 is important in the development and prognosis of cancers [21]. miR-486 is expressed at low levels in a variety of malignant tumors, including oral tongue squamous cell carcinoma [22], cervical cancer [23], lung cancer [24], papillary thyroid carcinoma [25], and esophageal cancer [26]. However, it is also highly expressed in some tumors, such as colon cancer [27], hepatocellular carcinoma [28], and OC [15]. In this study, we found that miR-486 was upregulated in OC tissues and cells; at the same time, interfering with miR-486 inhibited the proliferation, invasion, and cell cycle of OC cells and promoted their apoptosis. These results suggest that miR-486 plays a biological role in promoting the development in OC.
Further, the underlying regulatory mechanism of miR-468 was also investigated. Ma et al. have found that estrogen receptor signaling downregulates miR-486-5p and upregulates the target gene OLFM4, thereby slowing the development and progression of OC [15]. In the present study, CADM1 was confirmed to be a direct target of miR-486. CADM1 is a member of immunoglobulin superfamily, which can prevent malignant transformation and metastasis through the maintenance of epithelial cells [29]. CADM1 gene on chromosome fragment 11q23.2 was originally identified as a tumor suppressor gene in non-small-cell lung cancer, which was confirmed in nude mice [30]. CADM1 encodes an immunoglobulin superfamily cell adhesion molecule expressed in the brain, testis, lung, and various epithelial tissues [31]. CADM1 protein plays an important role in   Analytical Cellular Pathology epithelial cell adhesion through the homogenous transmission among adjacent cells [32]. However, its molecular mechanism in OC remains unclear. The results of cell rescue experiment confirmed that interference with CADM1 reversed the effect of miR-486 inhibition on OC cell functions. Therefore, it can be used as a biomarker and a potential therapeutic target in oncology. It has been reported that overexpression of CADM1 inhibits the progression of tumors [33,34]. These results are consistent with our findings, indicating that miR-486 regulates the development and progression of OC by targeting CADM1. At present, more and more scholars consider tumor as a kind of cellular periodic disease, which is closely related to a series of pathophysiological processes such as cell growth and proliferation, differentiation, apoptosis, repair, and carcinogenesis [35]. Cell cycle is a process tightly regulated by genes [36]; the abnormal cell cycle regulation will cause cell growth and proliferation out of control, resulting in tumor development or even metastasis [37,38]. Cyclins, CDKs, and CDK inhibitors constitute a network system to regulate at the critical restriction points [39]. Among them, CyclinD1, CyclinE, and CDK6 are the key to promote the cell cycle from the G1 phase to the S phase and to initiate the operation of cell cycle, which are most closely related to tumors. They are currently recognized as oncogenes. Their abnormal expression and function lead to the disorder of cell cycle regulation and cell proliferation out of control and then cause tumor development and metastasis and even related to the treatment and prognosis of cancer patients [40][41][42][43], while the function of p21 is the opposite [44]. Our study found that interference with miR-486 can inhibit the expression of CyclinD1, CyclinE, and CDK6 while promoting the expression of p21 in OC cells. At the same time, reduction of CADM1 expression can reverse the corresponding expression of proteins.
In summary, miR-486 is highly expressed in OC cells and tissues and miR-486 targets CADM1 to regulate OC cell biological functions such as proliferation, invasion, and cycle progression. These conclusions also need to be verified by subsequent animal experiments, and the collection of clinical tissue to analyze the relationship between miR-486 expression and clinicopathology. This study suggests that miR-486 may be a potential target for the treatment of OC and has a good clinical application prospect for the prognosis of OC patients.

Data Availability
The datasets supporting the conclusions of this article are included within the article.

Ethical Approval
This study was approved by the Medical Ethics Committee of Henan Provincial People's Hospital.