Estimating Nitrogen Availability of Heat-Dried Biosolids

As heat-dried biosolids become more widely produced and marketed, it is important to improve estimates of N availability from these materials. Objectives were to compare plant-available N among three different heat-dried biosolids and determine if current guidelines were adequate for estimating application rates. Heat-dried biosolids were surface applied to tall fescue (Festuca arundinacea Schreb.) in Washington State, USA, and forage yield and N uptake measured for two growing seasons following application. Three rates of urea and a zero-N control were used to calculate N fertilizer efficiency regressions. Application year plant-available N (estimated as urea N equivalent) for two biosolids exceeded 60% of total N applied, while urea N equivalent for the third biosolids was 45%. Residual (second-year) urea N equivalent ranged from 5 to 10%. Guidelines for the Pacific Northwest USA recommend mineralization estimates of 35 to 40% for heat-dried biosolids, but this research shows that some heat-dried materials fall well above that range.


Introduction
Heat-dried biosolids are convenient to use in a variety of applications.The Class A heat-dried product is suitable as a fertilizer on lawns and gardens as well as for agricultural crops.Heat-dried biosolids are easy to transport and handle and are applied like inorganic fertilizers, except at higher rates.
Because a large proportion of the nitrogen (N) in biosolids is in organic form, biosolids act as a slow-release N source, dependent on biological transformation of the organic N into available forms.Accurate estimates of the mineralization rate of biosolids N are critical to developing application rate recommendations that meet plant needs without compromising environmental quality.Smith and Durham [1] used laboratory incubation to compare five different biosolids sources with and without heat drying, and found that the mineralization rates of the heat-dried biosolids were more than double the undried (dewatered only) materials.This rapid mineralization more than compensated for the lower initial ammonium N in the heatdried biosolids.Rigby et al. [2] observed similar results in a field incubation, estimating mineralizable N from heatdried biosolids at twice that for dewatered biosolids.Mat-suoka et al. [3] and Moritsuka et al. [4] produced heat-dried biosolids in an experimental scale vessel reaching final temperatures of 120 and 180 • C.They found increased available N in the 120 • C heat-dried biosolids compared with undried biosolids in laboratory incubation and pot studies.Heat drying to a final temperature of 180 • C reduced available N.
Few quantitative field estimates of N availability from heat-dried biosolids have been published.Cogger et al. [5] estimated 32% and 44% first-year plant available N% (PAN) for two heat-dried biosolids compared with ammonium nitrate applied to tall fescue (Festuca arundinacea Schreb.) in western Washington State, USA.Plant-available N% from dewatered and air-dried biosolids ranged from 29 to 43% in the same study.Gavalda et al. [6] reported 45% PAN (estimated as urea N equivalent) in a field study on maize (Zea mays L.) in southwestern France.In a longterm comparison of one heat-dried and one dewatered Class A biosolids, Cogger et al. [7] found the heat-dried and dewatered biosolids had similar long-term N availability following repeated applications.
As heat-dried biosolids become more widely produced and marketed, it is important to improve our understanding of N availability from these materials.In this study we compare two heat-dried biosolids from new facilities in western Washington with Milorganite, a well-established heat-dried product from Milwaukee, WI, USA.Our objectives were to compare amount and timing of N availability among these materials and determine if current guidelines were adequate for estimating application rates.The experiment consisted of two trials on adjacent areas of the tall fescue stand.Trial A began in 2007 and Trial B in 2008.Each trial was a randomized complete block design with 10 treatments and four replications.Individual plots measured 1.6 × 4.6 m.Treatments included three heat-dried biosolids (Pierce County Soundgro, Sumner, and Milorganite) each applied at a single rate, but at two timings (all in April, or split between April and June) for a total of six biosolids treatments (Table 3).The remaining four treatments were three inorganic N (urea) rates and a zero-N control (Table 3).Biosolids and inorganic N treatments were applied by hand to the surface of the tall fescue stand with no incorporation.Each trial was continued through two growing seasons, to evaluate application year and residual year response to the biosolids applications.

Methods
Initial application rates were estimated to supply 250 kg ha −1 plant available N based on 40% availability of N from the heat-dried biosolids [9].Tall fescue showed less response to the Sumner biosolids than to Soundgro or Milorganite in a short-season preliminary experiment in 2006 (data not shown), so the Sumner biosolids were applied at higher rates in Trials A and B (Table 3).Rates for all biosolids in Trial B (2008) were adjusted downward based on the 2007 results.Urea N rates ranged from 90 to 270 kg ha −1 N split over four applications in 2007, and were increased to 112 to 336 kg ha −1 N split over five applications in 2008 and 2009.In the residual (second) year of both trials no biosolids applications were made, but all biosolids plots received 168 kg ha −1 N as urea split over five applications (Table 3) to maintain grass vigor and obtain values for grass yield and N uptake near the center of the urea fertilizer response curves.
At the beginning of the experiment soil pH was 6.5.Ammonium acetate extractable K was 125 mg kg −1 , Ca 5.5 cmol c kg −1 , Mg 0.4 cmol c kg −1 , and Bray-1 extractable P 255 mg kg −1 .Soil test P and Ca were adequate for grass forage production, while K was low, and Mg borderline [10].To ensure adequate levels of all nutrients except N, all plots received supplemental potassium (176 kg ha −1 K), sulfur (45 kg ha −1 ), and magnesium (22 kg ha −1 ) applied as KCl (0-0-62) and K 2 SO 4 •2MgSO 4 (0-0-22-22 S-11 Mg) each spring.The tall fescue was irrigated as needed to prevent moisture stress and maintain growth throughout the summer.Growing season rainfall, irrigation, and temper-atures during the experimental period are summarized in Table 4.
The tall fescue was harvested six times per year at the early boot stage using a small-plot forage harvester.A 1 × 4.6 m swath was harvested from each plot at a height of 5 cm.Because the initial biosolids applications were made after the first harvest of the application year, only five harvests are included in the application year experimental period for both trials.The second (residual) year includes all six harvests.The harvested forage from each plot was weighed wet, and a subsample (approximately 500 g) was collected and oven dried at 55 • C to determine dry matter.The dried subsample was then ground and analyzed for total N. Soils from selected plots (Soundgro and Sumner split application and 270 kg ha −1 urea N) were sampled in October 2007 to determine residual soil nitrate-N at the onset of the rainy season.Samples were collected in 30 cm increments to a depth of 90 cm using a hydraulic probe with a 4 cm diameter.A minimum of three subsamples per depth and plot were collected and composited.
Total N was determined for biosolids and grass samples using a combustion analyzer (LECO Instruments, St. Joseph, MI).Biosolids ammonium N and soil nitrate N concentrations were measured after extraction with 2 M KCl.Ammonium-N was determined using an automated salicylate-nitroprusside method and nitrate-N determined by an automated cadmium reduction method [11].

Data Analysis.
Nitrogen uptake was calculated as the product of the yield and N concentration of the grass tissue.Statistics for yield and N uptake from the biosolids treatments were computed by ANOVA using a factorial design and the SAS General Linear Models procedure [12].
Apparent N recovery N from the biosolids was calculated in a two-step process.First, we used a linear regression of the N uptake at the different urea rates to calculate the fertilizer uptake efficiency of urea and background soil N availability: where (i) N rate is the amount of urea N applied at each rate (kg ha −1 ), (ii) urea efficiency is the slope of the N uptake versus N rate regression (unitless) and represents the proportion of urea N taken up into the harvested fescue, (iii) background soil N (kg ha −1 ) is the intercept and represents fescue N uptake from the unamended soil, Separate equations were calculated for each trial each year.Because clover became established in the zero-N plots in 2008 and 2009 and added fixed N to the system, we did not include N uptake from those plots in the urea regression equations after 2007, using only the fertilized plots for those years.
Apparent N recovery is an estimate of N uptake from the biosolids.It is the difference between N uptake from the biosolids treatment and N uptake in the zero-N control plots (assumed to be background soil N).When expressed as a percentage of total N applied, it estimates the fraction of the biosolids N captured in the harvested plant tissue.We calculated apparent N recovery for the application year of each trial by subtracting the intercept of the appropriate urea regression from the biosolids N uptake for the same trial.Dividing by the biosolids total N applied gives the apparent N recovery percentage: where (i) treatment N uptake (kg ha −1 ) is the amount of N captured in the harvested portion of the fescue for each treatment, (ii) applied N (kg ha −1 ) is the total amount of N applied in each biosolids treatment.
For the residual year of each trial, we modified the apparent N recovery equation to account for the 168 kg ha −1 that was applied to all of the biosolids treatments that year: where (i) N 168 represents N uptake from the 168 kg ha −1 urea N treatment, derived from the appropriate urea N uptake regression for each trial.
Plant-available N (PAN) is an estimate of N release from the biosolids.PAN differs from apparent N recovery in that it includes available N that was not taken up into the harvested   part of the plant.This could include N in roots and crowns, or N lost to leaching or volatilization.PAN is used to compare N availability among different soil amendments.In this study we used urea N equivalent as a surrogate for PAN.Urea N equivalent (expressed as % biosolids N) is calculated by dividing the treatment apparent N recovery (%) by the urea efficiency (slope) for the appropriate trial and year Urea N equivalent % = biosolids treatment apparent N recovery % urea efficiency . (4)

3.1.
Tall Fescue Yield and N Uptake.Tall fescue yield and N uptake from the biosolids treatments in the application and residual years are shown in Tables 5 and 6.Lower yields in the Trial B application year compared with Trial A reflect lower rates of biosolids application (Table 1).No treatment interactions between biosolids source and application timing were observed for either yield or N uptake.There was no significant effect of biosolids source on tall fescue yield or N uptake during the application year for either trial (Table 5).The biosolids were not applied at uniform rates, however, as the rate of the sumner product was about 50% higher than Soundgro and Milorganite (Table 1).Biosolids source did affect yield in the residual year of Trial A and N uptake in the residual year of both trials with lower yield and N uptake from Milorganite (Table 6).Splitting the biosolids application over two dates had only small effects on full-season tall fescue yield or N uptake.The only significant effect was increased yield with split application in the application year of Trial B. Split  applications did result in more uniform yields across harvests in the application year (Trial A shown in Figure 1).

Biosolids Apparent N Recovery and Urea N Equivalent.
Urea N uptake regressions were used in the calculation of biosolids apparent N recovery and urea N equivalent.A separate urea uptake regression was calculated for each trial each year (Figure 2).Intercepts (background soil N) were similar across the regressions, but the slope (urea uptake efficiency) was significantly lower in the Trial B application year than in the other regressions.
Apparent N recovery % for the application year was similar across the two trials and was similar between Soundgro and Milorganite (Table 7).Apparent N recovery % was lower for the Sumner biosolids.Apparent N recovery % for Milorganite in this study (39%) was greater than that reported in previous research (33%) [5].Application year urea N equivalent averaged 62% for Soundgro and Milorganite in Trial A and 75% in Trial B (Table 7).The very high results in Trial B are likely an artifact of the low urea uptake efficiency observed in the fertilizer plots for the Trial B application year.Much less N was released in the residual year, with urea N equivalent estimated from 8 to 10% of N applied for the Soundgro and Sumner products, and 4 to 5% for Milorganite (Table 8).
Postharvest soil nitrate N was low in the samples collected in 2007, and not different among the treatments sampled, averaging 4.0 mg kg −1 in the 0 to 30 cm depth, 2.8 mg kg −1 at 30 to 60 cm, and 2.7 mg kg −1 and 60 to 90 cm.This is consistent with results from treated and control plots in previous biosolids experiments done under similar conditions [7] and indicates little potential for leaching loss from the plots.
First-year available N from Milorganite and Soundgro was higher than previously reported for heat-dried biosolids under field conditions [5,6].Similarly high first-year available N has been observed in laboratory incubations [1].They reported 58 to 59% available N from lab incubations of three heat-dried biosolids.The Sumner biosolids had lower available N, but still equal to or greater than observed for biosolids produced without heat drying.Second-year urea N equivalent in this study was similar to field PAN values for heat-dried biosolids reported previously [5,6].The heat dried biosolids all tended to release N rapidly, with most of the yield response occurring in the first two harvests following an application (Figure 1).
These results show that first year available N (as urea N equivalent) for Milorganite and Soundgro exceed 60%, which is greater than previously reported in the literature.Current guidelines for the Pacific Northwest of the USA recommend using mineralization estimates of 35 to 40% for heat-dried biosolids [9], but this research shows that at least some heat-dried materials fall above that range, meaning that these materials are effective at lower application rates.We expect these results can be extended to other temperate regions with adequate growing season rainfall or irrigation, based on the results of a previous multilocation field and modeling study of biosolids N availability [13].Future research is needed to determine the relationship between specific heat drying processes and available N to improve estimates of N release from heat-dried products.

1 )Figure 1 :
Figure 1: Comparison of single and split biosolids applications on tall fescue dry matter yield by harvest, Trial A application year.

Table 2 :
Particle size distribution of biosolids dry products.

Table 3 :
Biosolids and urea N fertilizer application amounts and timing for the ten treatments.

Table 5 :
Tall fescue dry matter yield and N uptake, Trials A and B, application year.

Table 6 :
Tall fescue dry matter yield and N uptake, Trials A and B, residual year.

Table 7 :
Apparent N recovery and urea N equivalent from biosolids, application year.Single and split application data pooled.

Table 8 :
Apparent N recovery and urea N equivalent from biosolids, residual year.Single and split application data pooled.