Antioxidant Action and In Vivo Anti-Inflammatory and Antinociceptive Activities of Myrciaria floribunda Fruit Peels: Possible Involvement of Opioidergic System

This work evaluated the antioxidant properties and in vivo antinociceptive and anti-inflammatory effects of extracts obtained from fruit peels of Myrciaria floribunda (H. West ex Willd.) O. Berg (Myrtaceae). This plant is popularly known in Brazil as Cambuí or camboim. Different extracts were submitted to comparative analysis to determine the content of selected phytochemical classes (levels of total phenols, flavonoids, and monomeric anthocyanins) and the in vitro antioxidant potentials. The extract with higher potential was selected for in vivo evaluation of its antinociceptive and anti-inflammatory action. Finally, the chemical characterization of this extract was performed by high-performance liquid chromatography (HPLC). MfAE (extract obtained using acetone as solvent) showed the higher levels of phenols (296 mg GAE/g) and anthocyanins contents (35.65 mg Cy-3-glcE/g) that were associated with higher antioxidant activity. MfAE also exhibited in vivo anti-inflammatory and analgesic propertiers. This fraction inhibited the inflammatory and neurogenic phases of pain, and this effect was reversed by naloxone (suggesting the involvement of opioidergic system). MfAE reduced the abdominal contortions induced by acetic acid. The HPLC analysis revealed the presence of gallic acid (and its derivatives) and ellagic acid. Taken together, these data support the use of M. floribunda fruit peels for development of functional foods and nutraceutics.


Introduction
Free radicals (and associated reactive species) are in general derived from normal metabolism and are crucial for redox signaling pathways and immune defense [1][2][3][4]. However, these molecules can interact with cellular structures leading to impairment of physiological systems [5][6][7]. In this sense, the oxidative stress has been associated with the etiology of several pathologies such as inflammatory disorders, chronic pain, and degenerative diseases [8][9][10].
Inflammation is a complex condition triggered by several stimuli (mechanical injuries, toxic compounds, tissue ischemia, and infectious agents) and marked by tissue alteration that allows the intense migration of immune cells to the inflammatory focus [11][12][13]. Usually, the release of proinflammatory substances (cytokines, nitric oxide, and other reactive species) is reduced after the eradication of stimuli, leading to the resolution phase [13,14]. However, in some situations, the resolution of inflammation is not achieved, resulting in excessive or inappropriate inflammation [11]. is condition is associated with several diseases such as cancer, diabetes, rheumatoid arthritis, and psoriasis [12,15]. In this case, the high levels of reactive species lead to tissue damage and organ dysfunction [2,4,5]. e excessive release of inflammatory mediators can result in the establishment of inflammatory pain [16,17]. For example, the cytokines TNF-α, IL-1β, and IL-6 (produced by macrophages and other immune cells) interact with nociceptive neurons and modulated central sensitization, pain, and hyperalgesia [18][19][20]. Furthermore, other substances produced by immune cells, such as histamines and prostaglandins, also play important roles in pain regulation [17,21,22]. In this context, pain and inflammation have a tightly regulated relationship, since the nociceptive neurons also regulate the inflammatory response [17,21,23].
Despite the number of anti-inflammatory and analgesic drugs available in the market, the treatment schedules of these clinical conditions are complex and pose huge challenges to the health systems worldwide [24,25]. is scenario points out the urgent need for the development of effective analgesic and anti-inflammatory drugs [26][27][28].
Several scientific evidences report that fruits are valuable sources of compounds with antioxidant properties, and their consumption may be beneficial to reduce the deleterious effects of inflammatory disorders and chronic pain [29][30][31][32]. In this sense, there is a growing interest in knowing the anti-inflammatory and analgesic properties of edible fruits (and their different parts) in order to provide more insights into supporting their use as functional foods and nutraceuticals [30,33,34].
Myrciaria floribunda (H. West ex Willd.) O. Berg (Myrtaceae) is native plant of South and Central America, which is distributed in different Brazilian biomes such as Amazon, Caatinga, Cerrado, and Atlantic Forest. Its fruits (known in Brazil as Cambuí or camboim) are edible and often consumed freshly, in juices or alcoholic beverages [35]. Some works have shown the pharmacological properties of different parts of this plant such as antioxidant, antimicrobial, and anticancer effects [36,37]. However, the pharmacological actions of the fruits were not properly addressed [35]. In particular, the beneficial aspects of the products derived from fruit peels of the M. floribunda have not been demonstrated.
is work analyzed the phytochemical composition along with the antioxidant, antinociceptive, and anti-inflammatory potentials of extracts obtained from fruit peels of M. floribunda.

Plant Extracts.
e fruits were washed with running water and manually separated into pulp, seed, and peel. Samples of the fruit peels were dried at 40°C and reduced to fine powder. e fruit peel powder (50 g) was extracted with 500 mL of ethyl ether (1 :10, w/v) in brown glass bottles with cap by vigorous shaking for 1 hour at room temperature (30°C). e extract was then filtered, and the residue was re-extracted twice more following the same procedure with 500 mL. After this process, the residue was extracted with other solvents (in the sequence chloroform, acetone, and methanol), and all extraction steps were repeated as described above. At each step, the obtained extracts were dried in a rotaevaporator at 40°C, generating extracts MfEeE (ethyl ether), MfCE (chloroform), MfAE (acetone), and MfME (methanol). Aqueous extraction was also performed with 10 g of the powder with 100 mL of distilled water (1 :10, w/v); then, the solution was filtered and lyophilized, generating the extract MfAqE.

Total Phenol Content, Total Flavonoid, and Monomeric
Anthocyanins.
e levels of total phenols were estimated using the Folin-Ciocalteu method [42], with some modifications. Samples of 20 μL of each extract (1 mg/mL) were mixed with 100 μL of the Folin-Ciocalteu reagent. After 3 minutes at room temperature, 80 μL of a sodium bicarbonate solution (0.7 M) was added. e reaction was kept in the dark for 2 hour at room temperature. e absorbance was measured at 735 nm using a microplate reader (BioTek uQuant MQX200). Methanol and distilled water were used as negative controls. Gallic acid was used as standard, and the results were calculated based on the calibration curve of gallic acid (10-100 μg/mL) and expressed as mg equivalent of gallic acid per gram of extract (GAE/g extract).
e flavonoid content was determined according to the colorimetric method of aluminum chloride [43].
e extracts were tested at the concentration of 1 mg/mL, and quercetin was used to obtain the standard calibration curve (10-100 μg/mL). e sample (100 μL) was mixed with 100 μL of the reagent [2% aluminum chloride (AlCl 3 ) in methanol]; after 1 hour in dark-room-temperature environment, absorbance was read against a blank of methanol or more distilled water, the reagent, at 420 nm. e results obtained were expressed as mg equivalent of quercetin per gram of 2 Advances in Pharmacological and Pharmaceutical Sciences extract (mg QE/g extract).
e experiment was done in triplicate.
e content of monomeric anthocyanins was determined by the differential pH method [44]. Potassium chloride buffer (KCl; 0.025 M; pH 1) and sodium acetate buffer (CH 3 CO 2 Na; 0.4 M; pH 4.5) were used. e sample (200 μL at 10 mg/mL) was mixed to 1.8 mL of each buffer. After 10 minutes, the absorbance was read at 520 nm and at 700 nm. e distilled water was used as control. e concentration of anthocyanin was calculated according to the following equation, and the results were expressed as cyanidin-3-glucoside equivalent (Cy-3-glcE): A � (absorbance 520 nm−absorbance 700 nm) at pH 1 −(absorbance 520 nm−absorbance 700 nm) at pH 4.5; MW (molecular weight) � 449.2 g/mol of Cy-3-glc; DF � dilution factor (0.2 mL of the sample was diluted to 2 mL, DF � 10); 1 � length in cm; ε � 26900, molar extinction coefficient, in L x mol −1 x cm −1 , for Cy-3-glc; and 10 � factor for conversion from g to mg.

DPPH Assay (Free Radical Sequestration).
For evaluation of the ability to sequester the DPPH (2,2-diphenyl-1picryl-hydrazyl) radical, an aliquot of 40 μL of each extract at different concentrations (8.0625 to 1000 μg/mL) was mixed with 250 μL of 1 mM DPPH solution (in methanol) for 25 minutes at room temperature and protected from light [45]. Gallic acid was the standard used as a positive control, and methanol was the negative control. A control solution (40 μL of the extract and 250 μL of the solvent used to dilute the samples) was used to rule out the possible interference of extract color. e absorbance of each solution was measured at 517 nm using a microplate reader (BioTek uQuant MQX200).
is mixture remained in the dark and at room temperature for 16 hours before use.
e ABTS radical solution was diluted with ethanol until an absorbance of 0.7 nm (±0.02) was obtained at 734 nm. Samples (10 μL) of the extracts (1 mg/mL) were mixed with 1 mL of the ABTS radical for 6 minutes and then read off a spectrophotometer at 734 nm [46]. Trolox was used as standard antioxidant (100 μM to 2000 μM).
e result was expressed as Trolox equivalent antioxidant capacity (TEAC) in μM TE/g extract.

Total Antioxidant Capacity (TAC). Samples (100 μL)
of the extracts (1 mg/mL) were combined with 1 mL of the reagent solution (0.6 M sulfuric acid, 28 mM sodium phosphate, and 4 mM ammonium molybdate). e tubes were incubated at 95°C for 90 minutes and then cooled to room temperature, and the absorbance of each reaction was read at 695 nm. e control reaction consisted in 100 μL of methanol mixed with 1 mL of the reagent solution [47]. e standard used was ascorbic acid (1 mg/mL). e result was calculated according to the formula below and was expressed as total antioxidant capacity (TAC) relative to ascorbic acid.
where S abs is the absorbance of the sample (extracts), B abs is the absorbance of control, and AA abs is the absorbance of ascorbic acid.

Determination of the Sequestration of the Superoxide
Radical. In this assay, the reaction mixture consisted of 300 μL of extracts at different concentrations (50-1000 μg/mL), 100 μL of NBT (nitrotetrazolium blue chloride) (1 mg/mL in DMSO solution), and 1 mL of alkaline DMSO (1 mL DMSO

Animals.
e experiments were conducted using male Swiss mice (30-35 g, 10 weeks) supplied by the Laboratory of Immunopathology Keizo Asami (LIKA).
e animals received food and water standard ad libitum with light/dark period of 12 h. Before each experiment (6-8 hours), animals were limited to a water-only diet to avoid foodborne interference with substance absorption.
2.9. Evaluation of Acute Toxicity. Acute toxicity of MfAE was performed according to the instructions of the Organization for Economic Cooperation and Development (2001). e acute toxicity of MfAE was analyzed in two steps. Phase 1: animals were divided into three groups (n � 3) that received MfAE at doses of 10 mg/kg, 100 mg/kg, and 1000 mg/kg. e animals are placed under observation for 24 hours to monitor their behavior and survival. Phase 2: MfAE was administered at higher doses (1600, 2900, and 5000 mg/kg; one mice per dose), and then the behavior and survival were observed for 24 hours [49]. e LD50 was calculated by the following equation: D 0 � highest dose that did not induce mortality and D 100 � lowest dose that resulted in mice mortality.

Anti-Inflammatory
Assay. e in vivo anti-inflammatory action of MfAE was evaluated by carrageenan-induced paw edema. For this, mice were allocated into four groups (n = 6) that received the following treatments: (i) MfAE at 50 mg/kg (p.o.), (ii) MfAE at 100 mg/kg (p.o.), (iii) vehicle (0.9% saline solution) (p.o.), or (iv) indomethacin at 20 mg/kg (p.o.). After 1 h, paw edema was induced by an injection of 2% carrageenan solution (15 μL/animal) into the subplantar region of the right hind paw [50]. As control, saline solution (0.9%; 15 μL) was injected into the left hind paw. Paw volume was measured using digital Vernier caliper at each hour for 5 hours [51]. Inhibition of edema was calculated by evaluating the difference between the volumes of right and left paws.

Abdominal Pain Induced by Acetic Acid.
e mice were divided into four groups (n � 6) and were treated as described above. e nociception was induced by an intraperitoneal injection of acetic acid (0.8%; 100 mL/10 g). Starting 5 minutes after acetic acid injection, the number of abdominal contortions was recorded for 10 minutes by the number of stretching movements [52].

Formalin Assay.
In this assay, the animals were divided into five groups (n � 6/group) that received the following treatments: (i) vehicle, (ii) MfAE at 50 mg/kg (p.o.), (iii) MfAE at 100 mg/kg (p.o.), (iv) indomethacin at 20 mg/kg (i.p.), and (v) morphine at 10 mg/kg (p.o.). After 1 h, the formalin solution (2.5% in 0.9% saline; 20 μL/paw) was administered into subplantar area of the right hind paw [53]. Mice were observed in a chamber with a mirror mounted onto three sides to allow the observation of the paws. e time (in seconds) spent for licking and biting the injected paw was measured as an indicator of pain. is response was measured for 5 minutes (first phase, neurogenic pain) and between 15 and 30 minutes after formalin injection (second phase, inflammatory pain).
To evaluate the possible involvement of the opioid system in the antinociceptive effect of MfAE, the mice were treated with naloxone (1 mg/kg, i.p.) 30 minutes prior to administration of MfAE (100 mg/kg, p.o.) and morphine (10 mg/kg, p.o.).

Statistical Analysis.
e in vitro assays were performed in triplicate in at least two independent assays. e results were expressed as mean (±standard error). e difference between means was tested for statistical significance using one-way or two-way analysis of variance (ANOVA) followed by Tukey's test for multiple comparisons. e IC 50 (concentration that inhibits 50%) was calculated by linear regression. In the statistical analysis of the results, the value of p < 0.05 was considered statistically significant.

Comparative Analysis of the Concentration of Total
Phenols, Flavonoids, and Monomeric Anthocyanins. In order to select the extract with the highest potential, we performed a comparative evaluation of the amount of phenols, flavonoids, and monomeric anthocyanins. e content of total phenols varied among the extracts from 7.54 mg GAE/g to 296.27 mg GAE/g (Table 3). e highest phenol content was obtained for MfAE (296.27 mg GAE/g) ( Table 3). e levels of total flavonoid content ranged from 0.74 mg QE/g to 16.62 mg QE/g (Table 3). e MfEeE showed the highest concentration (16.62 mg QE/g). Finally, the content of monomeric anthocyanins ranged from 4.34 mg Cy-3-glcE/g to 35.65 mg Cy-3-glcE/g (Table 3) and MfAE showed the highest content (35.65 Cy-3-glcE/g). In summary, MfAE showed the highest content of phenolic compounds and anthocyanins.
ese classes of compounds have been associated with several beneficial properties of foods and medicinal plants [55][56][57].

Comparative Evaluation of Antioxidant Activity.
Following, four antioxidant assays were employed to select the extract with the best potential (Table 4). In the DPPH assay, the lower IC 50 (Table 4). e highest antioxidant action exhibited by MfAE may be explained by its higher content of anthocyanins and phenolic compounds, since the levels of these phytocompounds showed positive correlations with the results obtained in the in vitro antioxidants assays (Table 4). In addition, MfAE presented a phytochemical profile with different classes of secondary metabolites, such as flavonoids, phenylpropanoids, triterpenes, saponins, and hydrolysable tannins. Taken together, the results suggest that MfAE is the most promising extract, leading us to select it for the in vivo anti-inflammatory and antinociceptive evaluation.

HPLC Analysis of the Acetone Extract of M. floribunda.
e chromatographic profile of the acetone extract from peels of the fruit (MfAE) is shown in Figure 1. e analysis revealed the presence of gallic acid (peak 1, with retention time of 7.56 minutes) and an indicative presence of ellagic acid (peak 7, with retention time 25.52 minutes). e other peaks (2 to 6) are gallic acid derivatives. e contents of the compounds were calculated as follows: 0.29 ± 0.0012 g% gallic acid, 0.33 ± 0.0204 g% gallic acid (derivatives, peaks of 2 to 6), and 0.54 ± 0.0012 g% ellagic acid. e evidenced peaks(gallic acid and ellagic acid) were identified by comparing the retention times and UV spectra of standards. Also, they were confirmed by spiking the sample with a small amount of the standards solutions. Both gallic acid and ellagic acid were previously detected in the fruit of M. floribunda [35].
Gallic acid and ellagic acid (and their derivatives) are usually found in fruits and other plant-derived products [55,58] with antioxidant activity, including those from Myrciaria genus [59,60]. Furthermore, some works have reported that these compounds are potent anti-inflammatory, analgesic, and neuroprotective agents [60][61][62].

Acute Toxicity.
e oral lethal dose (LD 50 ) of acetone extract was greater than 2000 mg/kg (LD 50 � 2154.06 mg/kg), therefore, it can be classified as a low toxic agent according to the Organization for Economic Cooperation and Development [63]. us, the doses of 50 mg/kg and 100 mg/kg were selected to be applied in the in vivo assays.

Anti-Inflammatory Activity.
e in vivo anti-inflammatory of MfAE was evaluated in a model where the formation of edema was induced by carrageenan. e animals treated with both doses of MfAE demonstrated significant reductions of paw edema, in all periods evaluated, when compared to vehicle-treated groups (p < 0.001) (Figure 2). Maximum inhibition (98.87%) was observed for 2 hours of treatment with MfAE at 100 mg/kg. For the dose of 50 mg/kg, the higher effect (93.75%) was observed after 5 hours.
Carrageenan-induced paw edema is an experimental model useful for the evaluation of new agents acting on acute inflammation [64][65][66].
is test is considered a biphasic Advances in Pharmacological and Pharmaceutical Sciences event; in the initial phase (1 to 3 hours), the release of inflammatory mediators such as histamine, serotonin, and bradykinin occurs, while in the late phase (3 hours to 4 hours), the synthesis of cyclooxygenase 2 (COX-2) isoform occurs, leading to prostaglandins and nitric oxide release [67,68]. MfAE reduced the edema during the five hours observed, suggesting that it acts in both phases of the acute inflammatory process. Further, extracts from other Myrciaria plants also exhibited anti-inflammatory action in this model [64,65]. e results suggest that the effect of MfAE in the formation of edema may involve multiple points such as the inhibition of the synthesis and/or release of inflammatory mediators, via COX or different specific enzymatic mechanism. e richness of polyphenols (flavonoids, anthocyanins) in MfAE has contributed towards the anti-inflammatory activity [60]. However, complementary studies based on these inflammatory mediators and COX inhibition should be explored, as well as phytochemical isolation.

Antinociceptive Activity.
e treatment with both doses of MfAE also significantly inhibited the number of contortions (95.16% and 95.59%, resp.), compared to vehicle (saline) (p < 0.001). ere were no differences neither between the different concentrations, nor in relation to the indomethacin control ( Figure 3). In this test, acetic acid induces nociception through a mechanism that depends on the resident cells (macrophages and mast cells) presented at the peritoneal cavity. ese cells release cytokines (TNF-α, interleukin 1β, and interleukin 8) that are involved in the contortion response [69]. However, this model cannot infer whether the antinociceptive action of MfAE is central or peripheral.       Regarding the formalin test, the results shown in Figure 4 reveal that in the first phase (neurogenic phase) of the test, only the concentration of 100 mg/kg showed a significant reduction of licking (86.52%; p < 0.001). Morphine (10 mg/kg) induced a reduction of 83.98% at this stage. However, in the second phase (inflammatory phase), both concentrations of MfAE had a significant reduction in licking time (p < 0.001).
e inhibition was 69.47% and 82.58% for MfAE at 50 mg/kg and 100 mg/kg, respectively. Positive controls, indomethacin (20 mg/kg) and morphine (10 mg/kg), inhibited 76.45% and 94.91% of licking, respectively. Naloxone reverted the effects of MfAE only in the neurogenic phase. e formalin test is a nociception model based on a biphasic pain response; it is a useful tool to investigate whether the action of new agents has an analgesic and antiinflammatory effect [70]. In the first phase (neurogenic pain) which occurs up to 5 minutes after the injection of formalin in the paw, the direct chemical stimulation on nociceptors activates the afferent fibers and leads to the release of substance P. In the inflammatory phase (15 to 30 minutes after the injection), the release of inflammatory mediators occurs, triggering a peripheral inflammatory response [53,71].
Centrally acting analgesic drugs (such as morphine) are able to inhibit both phases of the formalin test; in contrast, peripherally acting drugs (such as indomethacin) inhibit only the second phase [72]. Our results demonstrate that MfAE at the dose of 100 mg/kg can reduce both phases of the nociceptive response (p < 0.001). In the first phase, the analgesic effect of MfAE (100 mg/kg) was mediated by the action on the opioid receptors, as naloxone reversed the effect of MfAE.
is observation is attributed to the opioidergic mechanism that has been antagonized in the administration of naloxone (selective opioid receptor antagonist).

Conclusion
e results of this work show that the extracts from the peels of M. floribunda fruits are sources of compounds with antioxidant activity. e use of fruit peels, a possible residue of fruit consumption, represents a sustainable application of these natural products. In addition, the comparative analysis of antioxidant and phytochemical profiles allowed the selection of MfAE (a fraction rich in phenolic compounds and anthocyanins) as an agent high in pharmacological potential. MfAE also showed significant anti-inflammatory and antinociceptive activities (effect involving the opioidergic system). Considering the present results, the fruit peels of M. floribunda are sources of high-value bioactive compounds that may contribute to several beneficial effects associated with the consumption of this fruit. In this sense, the fruit peels can be used for development of functional foods and nutraceuticals.
Data Availability e data that support the findings of this study are available from the corresponding author upon reasonable request.

Conflicts of Interest
e authors declare that there are no conflicts of interest regarding the publication of this paper.