Gene therapy can be defined as incorporation of genetic material, that is, DNA or RNA, in the cellular gene regulation system, either to correct the expression of a malfunctioning gene or to modulate the cellular functions through expression of the newly incorporated gene. Nucleic acids (NAs) to correct/block malfunctioning genes have great therapeutic potential; however, their application is limited by their low cellular uptake, short half-life
The discovery of gene therapy with potential advantages over existing biochemical technologies has proven to be an indispensable tool for elucidating molecular pathways and phenotype/genotype relationships. Because of various limitations attached to the stability of DNA in biological milieu, several parameters are to be taken care of, for the therapeutic success of gene therapy, such as (i) DNA protection, (ii) high transfection efficacy, (iii) reduced toxicity and absence of nonspecific effects, (iv) high potency even at dosage of DNAs, (v) adaptation to various treatment regimens as well as diseases, and (vi) efficient vectors to bypass intracellular and extracellular barriers to reach their target tissue/organ.
In spite of much research being carried out with the aim to design different types of vectors for DNA delivery, safe and efficient delivery of DNA into target cells or organs still remains a big challenge. The DNA delivery is a multistep process where a series of extra- and intracellular barriers have to be bypassed for successful application and could be achieved with efficient vectors. For a vector to be an efficient delivery vehicle, it should consist of three different functional moieties: (i) a cationic polymer or lipid component to condense DNA and form complexes, which facilitates their endosomal escape after cellular uptake and facilitates effective unpacking of the complexes in the cytoplasmic compartment; (ii) a hydrophilic component, such as PEG, to impart solubility and stability to the complexes in the biological environment; (iii) a ligand that is specific for the target cell/tissue for enhanced target ability to impart more efficient cellular uptake by receptor-mediated endocytosis. Further, differences in angiogenesis and metastasis between cancerous and normal cells can be exploited to engineer nanoparticle-DNA complexes to be employed to target tumour cells and, henceforth, for designing target-specific vectors. With nanoparticle-DNA complexes gaining successful applications, polymeric nanoparticles are rapidly emerging as DNA delivery systems both
For nanoparticles to be designated as an ideal DNA carrier system, the nanoparticles must possess long circulation time, low immunogenicity, good biocompatibility, selective targeting, and efficient penetration to barriers such as the vascular endothelium and the blood brain barrier, self-regulating release without clinical side effects. The successful implication of DNA complexes in clinical applications requires exhaustive details pertaining to efficacy and pharmacokinetics of DNA-vector systems. There is still a huge amount of effort required in preparation of better polymers and in the development of better ways of encapsulating or complexing DNA with them. In this era of developing genetic therapeutics, there is a call for the collaborative work by academicians and industry groups in order to develop methods for preparation of more stable polymeric nanoparticles/DNA complexes and robust analytical methods to characterize formulations during formation and storage.