Omega-3 Polyunsaturated Fatty Acid Supplementation for Reducing Muscle Soreness after Eccentric Exercise: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

Purpose This systematic review and meta-analysis was performed to determine the effectiveness of Omega-3 polyunsaturated fatty acid (n‐3 PUFA) supplement on muscle soreness after eccentric exercise. Methods PubMed, EMBASE, CENTRAL, and ISI Web of Science were searched to identify randomized controlled trials (RCTs) that assessed the efficacy of n‐3 PUFA on muscle soreness after eccentric exercise. Mean difference (MD) and the associated 95% confidence interval (95% CI) were calculated by RevMan 5.3 to indicate delayed onset muscle soreness (DOMS) that measured two days after eccentric trainings. Subgroup analyses according to duration and daily dosage of n‐3 PUFA supplements before eccentric exercises were performed to determine whether these factors will influence the overall effect size. The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) approach was used to evaluate the certainty of evidence. The protocol of this systematic review and meta-analysis was registered at PROSPERO (CRD42018085869). Results 12 RCTs containing 145 subjects and 156 controls were included in this study. Meta-analysis revealed a significantly decreased DOMS (MD -0.93; 95% CI -1.44, -0.42; P = 0.0004) in n‐3 PUFA supplement groups, while no significant differences in isometric muscle strength and range of motion (ROM) were detected. However, the pooled effect size for DOMS was lower than the minimal clinically important difference (MCID) of 1.4 on the 10-unit VAS, suggesting that the effect size of less muscle soreness with n‐3 PUFA supplements did not appear to be clinically relevant. Conclusion There is low-quality evidence that n‐3 PUFA supplementation does not result in a clinically important reduction of muscle soreness after eccentric exercise. Isometric muscle soreness and range of motion were not improved by n‐3 PUFA supplementation either (low-quality evidence). To further elucidate the overall role of n‐3 PUFA on muscle damage in this area, large-scale RCTs are still needed.


Introduction
Omega-3 polyunsaturated fatty acids (n-3 PUFAs) are essential fatty acids rich in fish oils. The predominant components of n-3 PUFA including eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and alpha-linolenic acid (ALA), which can reduce the production and release of arachidonic acid and proinflammatory prostaglandins (PGs), are believed to have potent anti-inflammatory effects. Nutritional intervention with n-3 PUFA is widely believed to have the potential to alter the trajectory of a wide range of diseases including autoimmune diseases such as rheumatoid arthritis [1], inflammatory bowel diseases [2][3][4][5], and lupus nephritis [6], as well as cancer [7][8][9][10], cardiovascular diseases [11][12][13][14], metabolic disorders [15], and even neuropsychiatric decrements [16][17][18]. As for healthy individuals, current literatures focus on its implicit roles in metabolism [19] as well as skeletal muscle functions [20,21], among which a number of researches have also revealed its temporal effects in skeletal muscle, and even implied that it may play a role in delayed onset muscle soreness (DOMS) after eccentric exercise.
DOMS is recognized as a kind of common muscle discomfort occurring after unfamiliar exercises especially when a person is repeatedly exposed to high eccentric muscle contractions or unaccustomed exercise [22]. It increases within the first 24 h postexercise, and rises to peak between 24 to 48 hours [23][24][25][26] or 48 to 72 hours [23,27] according to different studies, then subsides and eventually disappears in 5 to 7 days [23,28]. Similar to acute muscle soreness, it presents with muscle stiffness, aching pain, and/or muscular tenderness [28][29][30]. Though the exact physiological mechanism of DOMS remains equivocal, researches have revealed its relations with damage of sarcomeres, ensuing swelling of damaged muscle fibers, and subsequent initiation of an inflammatory response [31][32][33][34]. So far, physical therapies, including postexercise massage [35,36], cryotherapy [37], low-level phototherapy [38], and vibration therapy [39,40], as well as the application of pharmacologic nonsteroidal anti-inflammatory drugs (NSAIDs) [41][42][43][44][45][46], are the predominant means employed to alleviate DOMS. Actually, since DOMS is widely accepted as a common self-treated condition, current studies have been turning to dietary interference, such as caffeine [47], ginger [48,49], and taurine [50,51], and some studies also revealed n-3 PUFA as a potential therapeutic agent to blunt DOMS. Despite the fact that majority of these studies are randomized controlled trials (RCTs), their sample sizes are relatively small, which though seems to be a common fault of trials in sports medicine, could not afford adequate statistical power. Therefore, to further elucidate the gainful benefit of n-3 PUFA in this field, we conducted a systematic review and meta-analysis based on the variable outcomes from current evidences.

Methods
This systematic review and meta-analysis was performed and reported following the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guideline [52]. The protocol for this study was registered with PROSPERO (CRD42018085869).

Literature Search Strategy.
A thorough literature search was conducted by searching PubMed, EMBASE, CENTRAL, and ISI Web of Science to identify potentially eligible articles about n-3 PUFA supplementation and muscle soreness after eccentric exercises. Four electronic databases were searched from their inception date to the latest issue (June, 2018) without language restriction. A combination of medical subject headings (MeSHs) and free terms were used based on the specifications of each database. An example of literature search strategy for PubMed was as follows: ("Fatty Acids, Omega-3"[MeSH] or omega-3 or n-3 polyunsaturated fatty acid or n-3 PUFA or "Eicosapentaenoic Acid" [MeSH] or Eicosapentaenoic Acid or EPA or "alpha-Linolenic Acid" [MeSH] or alpha-Linolenic Acid or Linolenic acid or "Docosahexaenoic Acids" [MeSH] or Docosahexaenoic Acids or DHA or "Fish Oils" [MeSH] or fish oil or fish oils) and (randomized controlled trial or random or randomly or placebo or controlled trial) and (eccentric contraction or eccentric training or eccentric exercise). The bibliographies of related systematic reviews were also searched. In addition, the reference section for each included study was also manually searched for inclusion of further eligible studies.

Inclusion and Exclusion Criteria
2.2.1. Types of Participants. Subjects enrolled in our systematic review were required to be adults that undertook eccentric exercise to induce muscle soreness. No restrictions on race, gender, and specific type of eccentric exercise were imposed. Volunteers were excluded if they consumed any kind of n-3 PUFA supplementation within the last 6 months. Participants were also excluded if they had engaged in specific upper limb or lower limb eccentric training or other kinds of resistance training in the previous 6 months.

Types of Intervention.
Participants were required to take n-3 PUFA supplements before and/or after a bout of eccentric exercise. The main components of supplements consumed should be n-3 PUFA (i.e., EPA, DHA, or linolenic acid), and a combination of EPA, DHA, and linolenic acid was deemed eligible for inclusion in our study.

Types of Control.
Participants in control groups were required to take placebo capsules that did not containn-3 PUFA. The main component of placebo capsules could be sunflower oil, corn oil, rice powder, and so forth. Both n-3 PUFA capsules and placebo capsules ought to be identical in appearance.

Types of Outcome.
The primary outcome of our study was DOMS after eccentric exercises, measured using the VAS; secondary outcomes included joint range of motion (ROM), isometric muscle strength, MVC, upper arm/thigh circumferences, skin temperature, jump performance, and M-wave latency as well as blood markers such as serum CK, CRP, IL-6, TNF-alpha, IL-1ra, cortisol, iron, and LDH measured after eccentric trainings. Considering DOMS and inflammatory reaction in response to muscle damage training peak within 1 to 2 days or 2 to 3 days postexercise, according to different studies, we combined the data measured two days after the exercises.

Types of Studies.
Only RCTs that assessed the efficacy of n-3 PUFA on muscle soreness after eccentric exercises were included. Non-RCTs such as case reports, case series, book chapters, and editorials were excluded.

Risk of Bias Assessment.
In order to assess the risk of bias among our included studies, the Cochrane Collaboration's tool is utilized, which is based on seven items: random sequence generation, allocation concealment, blinding of participants and personnel, blinding of outcome assessment, incomplete outcome data, selective reporting, and other sources of bias. Two reviewers (ZL and JZ) judged the risk of bias among studies independently, and the results were compared afterwards. In case of disagreements regarding the risk of bias judgment, discussion was conducted until a consensus was reached. Only data from studies with low or unclear risk of bias can be included in the analysis.

Data Extraction.
Two investigators (ZL and JZ) screened each article independently and were blinded to the findings of the other reviewer. Following the prespecified inclusion criteria, two reviewers performed a rigorous screening to identify eligible articles. Data were collected from these selected articles using a predetermined standardized data collection sheet, which included first author, year of the publication, country, sample size, demographic characteristics of participants in different groups, details of n-3 PUFA supplementation and control, detailed protocol of eccentric exercise, and main outcomes.
Discrepancies between two reviewers were resolved through discussion until a general consensus could be reached. The third review author (WZ) was sought for opinions if a consensus could not be reached.

Data Synthesis.
For DOMS and other continuous variables measured two days after eccentric trainings, mean difference (MD) and the associated 95% confidence interval (95% CI) were calculated using the Rev Man 5.3 (Copenhagen: The Nordic Cochrane Centre, The Cochrane Collaboration, 2014). Standard mean difference (SMD) and the associated 95% CI were calculated for continuous variables using the same methodology if the outcomes were measured using different ways. The chi-squared test and the Higgins I 2 test were used to assess the heterogeneity among studies (P > 0:1 and I 2 < 50% indicate acceptable heterogeneity) prior to the combination of data from an individual study [53]. Regardless of the between-study heterogeneity detected, the random-effect model was selected for statistical analysis because of the variety of types of eccentric exercises and dosage of n-3 PUFA supplements among the included studies; the random-effect model is preferable in the presence or anticipation of interstudy variances [54].
Power analysis of our meta-analysis was performed using the Power and Sample Size Calculation (PS) program (https:// biostat.mc.vanderbilt.edu/wiki/Main/PowerSampleSize) to evaluate whether our current study could offer adequate power to detect the beneficial effect of n-3 PUFA on DOMS at a level of significance of 0.05. Subgroup analyses according to duration and daily dosage of n-3 PUFA supplements before eccentric exercises were performed to determine whether these factors will influence the overall effect size. The leave-oneout sensitivity analysis was conducted by removing each included study in turn and reevaluating the resulting effect on pooled results to test the robustness of the summary estimate. Begg's rank correlation test and Egger's linear regression test as well as funnel plots using Stata version 12.0 (StataCorp LP, USA) were used to assess the publication bias [55].
2.6. GRADE Approach. The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) approach was used to evaluate the certainty of evidence for each outcome measurement [56]. In this approach, evidence from RCTs was initially regarded as "high quality" but could be downgraded to "moderate quality," "low quality," or "very low quality" depending on the presence and seriousness of five categories of limitations: limitations in the study design and implementation (risk of bias), indirectness of evidence, high and unexplained heterogeneity of results, imprecision of results, and high probability of publication bias. Two independent reviewers (ZL and JZ) finished the evaluation independently. Any discrepancy was resolved through discussion until a common consensus was reached. Otherwise, the third reviewer (WZ) was consulted for help. A table for the summary of findings was generated to explain the final results.

Literature
Search. An initial search yielded 58 potential citations, among which 16 were from PubMed, 5 were from EMBASE, 24 were from ISI Web of Science, and 13 were from CENTRAL. 27 citations were deleted because they were duplicates. After screening the titles and abstracts of the remaining 31 citations, 15 studies were selected and retrieved for full-text assessment based on the predetermined inclusion criteria, among which 3 were excluded with reasons, 2 were unrelated, and 1 contained duplicate data. Finally, a total of 12 RCTs [57][58][59][60][61][62][63][64][65][66][67][68] were considered eligible for inclusion and went into qualitative and quantitative syntheses. The literature screening process is presented in Figure 1.

Main Characteristics of Included
RCTs. 145 experimental subjects as well as 156 controls, covering Caucasian and Asian ethnic groups, regardless of genders, were included in this study. Of the 12 included trials, 5 were from USA [57,58,62,64,66], 3 were from UK [59][60][61], 2 were from Japan [65,68], 1 was from Germany [67], and 1 was from Iran [63]. All trial results were published between 2002 and 2017, with individual sample sizes ranging from 14 to 41. Participants included were mostly young adults with ages ranging from 18.2 to 33.5 years old. Detailed information for n-3 PUFA supplement and protocols of eccentric exercises, as well as main outcomes measured in each study are summarized in Table 1. 3.3. Risk of Bias. Cochrane Collaboration's tool was utilized for the assessment of risk of bias among included RCTs. All the studies selected in our study reported the suggested randomization, while only two studies reported the generation of random sequences. Details about allocation concealment were adequately provided by Jakeman et al. [61], whereas the other studies failed to report the procedure of allocation concealment. Although all the studies used placebo capsules that were identical with the n-3 PUFA capsules in appearance, only two studies [59,61] provided sufficient information about the blinding of participants and personnel and the blinding of outcome assessment. Only four studies [57,58,64,66] reported adverse events that happened during the follow-up period, so the remaining eight studies were judged to have high risk of bias in terms of incomplete outcome data. Overall, four studies [57,58,64,66] were judged to have moderate risk of bias, while the other eight studies were judged to have high risk of bias. The detailed information about reviewers' judgments about each risk of bias item is presented in Figures 2 and 3 .  Figure 4); the random-effect model was selected as previously described. Based on the result of power analysis, our meta-analysis had a power of 0.942 to detect the gainful benefit of n-3 PUFA on DOMS after eccentric exercise. Test for heterogeneity showed medium heterogeneity across included studies (Tau 2 = 0:23; Chi 2 = 13:01; df = 8; P = 0:11; I 2 = 39%). In our current study, we used 1.4 as the minimal clinically important difference (MCID) for pain reduction based on the 10-unit VAS [69]. It should be noticed that the effect size of less muscle soreness with n-3 PUFA was lower than the MCID, suggesting that the statistically significant difference in pain reduction was very unlikely to represent meaningful or important differences in clinical practice.

Publication Bias and Sensitivity
Analysis. Funnel plots for DOMS and isometric muscle strength were visually symmetrical (Figures 9 and 10 ), which indicated acceptable publication bias; Begg's test (DOMS: z = 0:31, P = 0:754; isometric muscle strength: z = 0:38, P = 0:707) and Egger's test (DOMS: t = 0:10, P = 0:926; isometric muscle strength: t = −0:60, P = 0:583) also confirmed no statistically significant publication bias. A funnel plot was not generated for ROM because only 4 studies reported ROM as an outcome measurement. Sensitivity analysis confirmed the robustness of results of the meta-analysis because the removal of any individual study did not change the overall estimate (detailed data not shown).     [58] measured CRP level in serum, both of which demonstrated no significance between the n-3 PUFA and control groups, although Phillips et al. [66] observed that the treatment group had a reduction in serum CRP of borderline significance compared to the placebo group.

Other
Serum IL-6 was analyzed by 7 studies. Both Phillips et al. [66] and Tsuchiya et al. [68] reported that serum IL-6 reached the peak at 3 d postexercise. Four out of 7 studies found a significant decrement in the n-3 PUFA groups compared to the placebo groups [58,[66][67][68], while others exhibited no differences [61,62] or even an upregulated IL-6 level [60]. Data on TNF-alpha level was presented in 3 studies, 2 of which indicated no effect of n-3 PUFA on blood TNF-alpha level [62,68], while 1 exhibited a significantly lower level in an experimental group after 2 h postexercise [64]. Two studies tested the cortisol level with conflicting results. One study reported a significant reduction of cortisol level in the n-3 PUFA group at both 24 hours and 48 hours after exercise [63], while the difference was not observed in the other study [62].
n-3 PUFA was reported to have no significant effect on other blood indicators, including IL-1ra [58], iron [62], and LDH [66]. On the other hand, IgA concentration was reported to be significantly lower in the n-3 PUFA group, and the IgA peak time was also 24 h earlier in the n-3 PUFA group [63]. In addition, blood troponin I and myoglobin peaked at 24 h and 72 h, respectively, after eccentric exercise, and both of them attenuated significantly in the n-3 PUFA groups [64].

Muscle Swelling.
Seven included studies have stated the effect of n-3 PUFA on postexercise muscle swelling which was assessed by measuring upper arm circumference or thigh circumference. Arm circumference increased by~2% after eccentric exercise, suggesting that swelling/edema was present [60]. However, except for Lenn et al. [62] who reported PUFA's preventive role in increased upper arm circumference after exercise and Tartibian et al. [67] who exhibited the opposite trend in thigh circumference, most articles illustrated no significant effect of n-3 PUFA in upper arm/thigh circumferences [57,[63][64][65]68].

Skin Temperature.
For skin temperature evaluation, only one study conducted by Corder et al. [57] presented no significant change between baseline and 48 h follow-up in either group, which indicated that inflammatory response in local muscle did not increase skin temperature.

Jump Performance.
With regard to jump performance, Jakeman et al. [61] found that participants who consumed a large dose of n-3 PUFA had better squat jump performance as well as countermovement jump performance than the control subjects. [65]. The outcome indicated that M-wave latency was significantly longer in the control group than in then-3 PUFA group immediately after the exercise.

M-Wave Latency. Only Ochi et al. measured M-wave latency of the biceps brachii to evaluate motor nerve function
3.5.6. GRADE approach. The evidence profile of different outcome measurements using the GRADE approach is shown in Table 2. Since only RCTs were considered for this meta-analysis, an initial rating of "high" was assigned to all outcome parameters which could then be downgraded according to the presence and seriousness of the following five factors: risk of bias, inconsistency, imprecision, indirectness, and publication bias. In our current meta-analysis, the certainty of evidence for all the three outcome parameters was downgraded by two levels due to critical study limitation (the majority of studies had study design features that carried a high risk of bias due to attrition bias) and imprecision (the

Discussion
The current systematic review and meta-analysis with 12 RCTs involving 145 experimental subjects and 156 controls suggested that n-3 PUFA supplementation was associated with less muscle soreness after exercise, but n-3 PUFA supplementation did not influence isometric muscle strength as well as ROM. Furthermore, since no consensus has been reached on the optimal dosage of n-3 PUFA and supplement duration, subgroup analyses based on dosage and supplement duration were also undertaken, with corresponding outcomes indicating that dosage as with supplement duration did not influence the effects significantly. However, we found that the pooled effect size of pain reduction was lower than the MCID of 1.4 units that we used in our current metaanalysis. Thus, the statistically significant difference of DOMS favoring n-3 PUFA supplementation was very unlikely to equate to meaningful or important changes in practice. Besides, we also summarized the actions of n-3  BioMed Research International PUFA on some other secondary outcomes like blood indicators and upper arm/thigh circumferences. But because of insufficient data as well as heterogeneity of treatments and endpoint measures, these secondary outcomes were not appropriate for quantitative data syntheses.
For decades, researches have demonstrated the interdependent association between physical fitness and cardiovascular disease risk status among adults [70,71], which, of note, has also been reported in children and adolescents [72], persisting even after adjustment for fatness as well as other confounding factors [73] and could track moderately well from childhood and adolescence into adulthood [74]. Considering the indisputable beneficial effects of physical exercises, the American College of Sports Medicine (ACSM) recommends a comprehensive program of exercise including cardiorespiratory, resistance, flexibility, and neuromotor exercise of sufficient volume and quality for apparently healthy adults of all ages [75]. As a matter of fact, it might     BioMed Research International be difficult at the beginning for those who remain exercise inactive, and DOMS is among the most common excuses for not participating. Under such circumferences, to alleviate such discomfort with dietary constituents and supplements would be a far more appealing choice, since for most healthy individuals, professional physical therapy and NSAID medication might be more like a fuss.
n-3 PUFA, well known for its anti-inflammatory effect, has been examined extensively for a great range of health outcomes as well as prevention of a wide array of diseases. As a nutritional factor that may reduce pain caused by various incentives, n-3 PUFA also received lots of attentions for the treatment of exercise-related muscle damage. This study provided combined evidence to show that temporal intake of

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BioMed Research International isolated n-3 PUFA could alleviate DOMS caused by strenuous exercises regardless of differences in the eccentric exercise protocols and/or muscle groups used, although no statistical difference was observed in ROM and isometric muscle strength. Moreover, effects on MVC and upper arm/thigh circumferences still remained equivocal, which might be due to the aforementioned differences in supplementation dose/duration as well as varying modes of exercise. In this respect, though there was insufficient evidence to say that n-3 PUFA could alter the condition of edema/swelling and muscle damage, or physical performances postexercise, its protective role in alleviating DOMS after eccentric exercise was somehow obvious. Both n-3 PUFA and n-6 PUFA belongs to PUFA family, which have two fundamental physiological functions in humans. On the one hand, they present as structural phospholipids in all cytomembranes and contribute to membrane functions. On the other hand, they are the primary precursors of the eicosanoids. However, n-3 PUFA and n-6 PUFA are transformed into corresponding eicosanoids each with exclusive functions. n-3 PUFA is believed to decrease the activity of n-6 PUFA arachidonic acid (ARA), a fatty acid that is related to the production of inflammatory mediators [76]. Though we did not perform meta-analysis of blood cytokine levels due to divergent evaluating methods, n-3 PUFA appeared to have limited effects from the summarized results mentioned above, and whether this results from a considerably short supplement duration ofn-3PUFA or suggests different mechanisms involved in the release of cytokines and muscle injury still remain to be further discussed.
A previous meta-analysis showed that n-3 PUFA could blunt inflammatory joint pain, though to gain a therapeutic effect, a minimum of 3 months' supplement duration at a dose of 2.7 gn-3 PUFA was required [77]. Still, another metaanalysis exhibited a moderate analgesic effect of n-3 PUFA in chronic pain, especially dysmenorrhea. Furthermore, a larger effect was observed for low doses and short periods of treatment [78]. When it comes to healthy individuals, though without evidences, it has been far long recommended by clinicians for different populations including athletes to consume 1000-2000 mg per day of n-3 PUFAs [79]. As majority of previous studies utilized daily large doses of n-3 PUFA (>1000 mg) to reveal their biological functions in inflammatory diseases and cancer, as well as in human health in general [80], this study went further by discussing the influences of both large (>1000 mg/d) and small (<1000 mg/d) dosage supplements, as well as >1 w and <1 w intake duration, and came out with the idea that the effect of n-3 PUFA on DOMS might not be dose-and duration-dependent, indicating a possibly more cost-effective way to ameliorate DOMS.
In order to assess the quality of evidence from our included studies, we used the GRADE approach which takes five categories of limitations (risk of bias, indirectness, inconsistency, imprecision, and publication bias) into consideration. All the included RCTs were initially rated as "high quality" and then downgraded based on the presence and seriousness of the limitations. We downgraded the quality of evidence for all outcome measurements by one level due to the high risk of bias (the majority of studies did not report adverse events associated with n-3 PUFA supplementation). No downgrading was performed for indirectness. No downgrading was done for all the three outcomes since the estimate of heterogeneity was acceptable (DOMS: I 2 = 39%; isometric muscle strength: I 2 = 3%; and ROM: I 2 = 16%). Downgrading for imprecision was supported by a relatively limited number of participants, since none of the outcomes had participants of more than 400. Begg's test along with Egger's test suggested no publication bias. In summary, all the three outcomes were graded as low quality. In other words, further research is very likely to have an important impact on our confidence in the estimate of effect and is likely to change the estimate. Considering the clinically marginal effect of n-3 PUFA supplementation on DOMS, despite the low-quality evidence from the included RCTs, it is still too early to recommend to young sport beginners to take n-3 PUFA supplements to reduce muscle soreness after exercise.
Although we have collected existing evidences based on RCTs and provided a more comprehensive evaluation, there are still several aspects waiting to be improved in the current study. Firstly, the studies included contained relatively small sample sizes, resulting in reduced statistical power. Secondly, based upon the findings of our current meta-analysis, the effect of n-3 PUFA supplementation on muscle soreness appeared to be clinically marginal. Furthermore, we failed to further achieve a better choice of supplement dosage or duration due to the limited number of included studies. Also, risk of bias assessment indicated that the majority of studies had flaws in study design and implementation (most studies did not report adverse events associated with n-3 PUFA supplementation). Thirdly, with the exception of Lenn et al. [62], all included trials studied only male or female subjects, thus reducing the generalization of the outcome. Lastly, subjects were basically young adults who practically participated in no resistance training within 6 months, so the effect in athletes or in the middle aged and elderly remain to be further discussed. Under such scenario, we call for large-scale, wellmodified RCTs which carefully evaluate dose and durationresponse relationships and involve roles of n-3 PUFA on DOMS as well as other indicators of muscle damage and inflammatory status.

Conclusion
Low quality of evidence suggested that n-3 PUFA supplementation was associated with significantly reduced DOMS after eccentric exercise, but the reduction in pain was unlikely to be clinically important. There is low-quality evidence that n-3 PUFA does not improve isometric muscle soreness and range of motion after exercise. Existing evidence on the potential preventive effect of PUFA on other measurements of muscle damage and inflammatory status are too limited to lead to conclusions. Taken together, taking n-3 PUFA supplements is not an effective way to reduce DOMS after eccentric exercise. Delayed onset muscle soreness ROM: Range of motion RCT: Randomized controlled trial SMD: Standard mean difference MD: Mean difference 95% CI: 95% confidence interval.

Data Availability
This is a systematic review and meta-analysis of published papers, and data could be found online.

Conflicts of Interest
The authors declare that they have no conflicts of interest.

Authors' Contributions
WZ conceived the idea of this study and made the final version of this manuscript. He had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. ZL and JZ did the literature search and evaluated the data. ZL was involved in revising the final manuscript, including some important intellectual contents and grammatical mistakes in our original study.