The Effects of Passive Simulated Jogging on Parameters of Explosive Handgrip in Nondiabetics and Type 2 Diabetics: A Single Arm Study

Aims Type 2 diabetes (T2D) is associated with sarcopenia and decreased muscle strength. Explosive and isometric voluntary handgrip strengths (EHGS and HGS) are frequently utilized methods to ascertain health status and a marker of overall muscle strength. We have previously shown that a portable, motorized device, which produces effortless, rapid stepping in place (passive simulated jogging device (JD)), improves glucose homeostasis. This study quantitatively evaluated the effects of JD in modifying parameters of the modified EHGS curve in T2D and nondiabetic (ND) subjects. Methods Twenty-one adult participants (11 ND and 10 T2D) (mean age: 41.3 ± 13.5 yr) performed a modified explosive handgrip strength (EHGS) test on study day 1 followed by daily use of JD (90 min per day) for 7 days. The EHGS was repeated after 3 and 7 days' use of JD (JD3 and JD7) and 3 days after completion of JD (Carryover). EHGS curves were analyzed for the following: maximal peak force value (MAX); rate of force development at 25%,75%, and 90% of maximum force; and maximum force (RFD25%, RFD75%, RFD90%, and RFDmax); time to 90%, 75%, and 25% of maximal force (t90, t75, t25) and time to maximal force (tmax); and the integrated area under the curve for force vs. time until task failure (iAUCTF); and fatigue resistance times at 50% and 25% of maximal force (FR50 and FR25) and fatigue resistance time to task failure (FRTF). Results At baseline, T2D had lower MAX compared to ND. There were no differences at baseline for force development time or fatigue resistance time between T2D and ND. In both T2D and ND, 7 days of JD increased FR25 and FRTF and iAUCTF compared to baseline. Conclusion JD for at least 7 days prior to EHGS increased time to task failure (fatigue resistance) and iAUCTF of the force-time curve. JD is a reasonable intervention to decrease sedentary behavior and improve muscle fatigue resistance under various clinical and nonclinical scenarios. This trial is registered with NCT03550105 (08-06-2018).


Introduction
Diabetes is a public health threat, and specifically, type 2 diabetes (T2D) has markedly increased in prevalence globally. T2D increases the risk of cardiovascular disease, death, morbidity, and poor functional outcomes [1][2][3]. Type 2 diabetics also demonstrate functional impairment with declined skeletal muscle strength [4,5]. Sarcopenia is an age-(primary) or disease-(secondary) related degenerative skeletal muscle disorder, characterized by a progressive and generalized decrease in muscle mass, strength, and function [6]. The latter is associated with impairment of the individual's ability to perform activities of daily living, increase in risk of falls, fractures, and mortality [7][8][9][10][11]. Sarcopenia has been associated with physical frailty, low quality of life, and cardiometabolic disease. The prevalence of sarcopenia has been estimated to be from 10-40% in healthy men and women aged ≥60 [12]. T2D is associated with an increased risk of sarcopenia (OR: 1.55 [CI: 1.25, 1.92]) [13], and the prevalence of sarcopenia in T2D is increased with a reduction in physical activity [14].
We have previously shown that a portable, motorized, self-administered, noninvasive movement technology, which produces effortless, rapid stepping in place while sitting or lying down (passive simulated jogging device JD, Gentle Jogger, Sackner Wellness Products LLC, Miami, FL 33132) improves glucose homeostasis in T2D and nondiabetic subjects, decreases spontaneous postprandial fluctuations/spike in blood glucose of non-diabetics, and decreases sedentary time [15,16]. Unpublished observations from our laboratory using a diabetic animal model showed that JDtreated animals had significantly larger skeletal muscle force generation and increased in time to task failure compared to nontreated.
Handgrip strength (HGS) provides a clinical tool and characterization of overall muscle strength and upper limb function [17]. HGS is related to diverse health conditions, informative of muscle mass, nutritional status, population health status, predictive of mortality, physical function, and hospital length of stay [17][18][19][20]. Additionally, meta-analysis of observational cohort studies showed that HGS might be a risk indicator for T2D in the general population and an independent predictor of cardiovascular outcomes in diabetes mellitus [21,22]. Recent systematic review and metaanalysis showed that T2D is associated with decreased handgrip strength compared to euglycemic state [13]. HGS can be measured using various protocols; one such protocol is the explosive handgrip strength (EHGS) which has been used to assess the force development phase of the HGS curve. The force developmental phase (initial phase until reaching the maximal force of the force-time curve) of the EHGS reflects muscle contraction speed and strength [23], may better assess neuromuscular transmission, may be more sensitive to detect acute and chronic changes in neuromuscular function [24], and is a predictor of functional mobility in the elderly [25]. Additionally, the aging process appears to reduce the explosive grip force-generating capacity before affecting the peak force [26].
This single arm study was carried out to quantitatively evaluate the effects of JD in modifying parameters of the modified EHGS curve in T2D and nondiabetic (ND) subjects.

Institutional Review Board
Approval. This study and its informed consent were approved by the Western Institutional Review Board (WIRB) (WIRB, Puyallup, WA 98374-2115) (WIRB) approved on April 2018 (No. 1184829). The study is registered at ClinicalTrials.gov NCT03550105 (06-08-2018) and conducted between September 2018 and May 2019. This study was part of a larger study that evaluated daily glycemic response, muscle strength, and endurance in healthy volunteers and type 2 diabetics. This study was designed as a nonrandomized single-arm study, in which each subject served as his or her own control. The sections of the study reported here are the effects of JD on parameters of the modified explosive handgrip. The inclusion criteria in the current protocol were healthy participants recruited from personal contact with normal fasting blood glucose and ages between 25 and 85 years. The exclusion criteria included inability to provide informed consent, interference with the placement of a continuous glucose-monitoring device (CGM) during the study period, and lack of compliance with the JD protocol. All participants were provided with approved informed consent forms and given the opportunity to ask questions. The CONSORT checklist and flow diagram are found in Electronic Supplemental Material File (ESM 1_File).

Passive
Simulated Jogging Device (JD). The portable JD incorporates microprocessor-controlled, DC motorized rapid movements of foot pedals placed within a plastic chassis to repetitively tap on a semirigid surface for simulation of locomotion while the subject is seated or lying in a bed. The device, which has been previously described and depicted, weighs about 4.5 kg with dimensions of 34 × 35 × 10 cm and can be used in supine or seated postures [15,16,27,28]. Its foot pedals alternate between right and left pedal movements to actively lift the forefoot upward about 2.5 cm, followed by active downward tapping with a semirigid bumper, simulating the feet impacting the ground. Each time the moving foot pedals strike the bumper, a small pulse is added to the circulation as a function of pedal speed. The present study protocol used JD speed of~190 steps in place per min [28,29].

Participants.
A convenience sample of eleven ambulatory nondiabetic (ND) individuals without a prior history of diabetes who had never taken either insulin or oral diabetic medication and 10 diabetics subjects (type 2 diabetes (T2D)) were enrolled and gave their informed consent to participate. There were no attempts to modify diet or physical activity, and all participants were told to maintain their normal exercise routine if any. All participants received financial remuneration for their participation. To gauge the activity level of the participants, the International Physical Activity Questionnaire (IPAQ) short form was obtained in seven of the eleven ND and all ten of the T2D. The IPAQ scores physical activity levels as low, moderate, or high [30]. BMI was computed to characterize participants as follows: BMI normal weight-18.5 to 24.9, overweight-25 to 29.9, and obese-30 or more. Demographics are shown in Table 1 BioMed Research International with back support and fixed arms, elbows flexed at 90 degrees and close to the body with the forearm and wrist in a neutral position, and thumbs up supported by the fixed arm of the chair. It has previously been shown that instructions are important for the evaluation of the force production of the static explosive grip [31][32][33]; therefore, all participants were given the instruction to exert maximal force as fast and as forcefully as possible immediately after hearing an audio cue and to maintain that grip for as long as they could [34]. Standardized audio encouragement cues were given to the participants throughout the entire test to continue to "squeeze fast and hard." To minimize any bias, the audio cues maintained the same volume intensity throughout the entire period and for 30 sec after the subject achieved task failure and thus completed the test. The modification to the standard EHGS procedure involved asking the participants to continue to squeeze until task failure.
To familiarize the participants with the EHGS procedure, two practice sessions were done on the recruitment day. One or two days later (study day 1), participants arrived to the testing center in the morning. The participants carried out one practice session of the modified EHGS. One hour later, a baseline (BL) measurement of EHGS was performed in duplicate with at least 30 min between each measurement. Participants were taught the operation of the passive simulated jogging device (JD) and requested to use it three times per day for 30 min sessions at 190 pedal steps in place per minute, amounting to greater than 10,000 pedal steps in place per day, for a total of 7 days. To verify compliance with JD use, they were asked to take photographs of the JD monitoring screen at the end of each session daily with a loaned iPhone and to deliver the iPhone to the study coordinator. The participants were instructed to continue their usual diet and physical activity if any and asked not to consume coffee or caffeinated drinks for at least 12 hr prior to arrival at the study center. The modified EHGS was again performed in duplicate after 3 and 7 days use of JD (JD3, JD7) and again 3 days after completion of JD (Carryover). Figure 1 summarizes this protocol.

Data
Analysis. The modified EHGS curves were analyzed for the following parameters: maximal peak force value 3 BioMed Research International (MAX) and rate of force development (RFD) defined as the slope of the force-time curve (Δforce/Δtime) at 90%, 75%, and 25% of the maximal force and maximal peak force (RFD 90% , RFD 75% , RFD 25% , and RFD max ); time to 90%, 75%, and 25% of maximal force (t 90 , t 75 , t 25 , and t max ); and fatigue resistance time, defined as the time at which grip strength decreases to, 50% and 25% of maximal force (FR 50 and FR 25 ), fatigue resistance time to task failure (FR TF ) [32,35], and integrated area under the curve for force vs. time for the entire curve until task failure (iAUC TF ) [36]. ANOVA was applied to the data set, with post hoc analysis using Least Significant Difference (LSD) and Dunn's Multiple Comparisons (Dunn) for nonparametric data. ANCOVA analysis was also applied to the data using baseline data, age, and gender as covariates (Statistica Software, Statsoft, TIBCO Software Inc., Palo Alto, CA). Graphs were plotted using GraphPad Prism 8 (GraphPad Software, San Diego, CA). Significant differences between means were taken as p < 0:05. To ascertain the effect size of the JD on both T2D and ND, we computed the nonparametric Common Language Effect Size (CLES) for significant variables: FR 25 , FR TF , and iAUC TF . The value represents the probability that a value chosen randomly from the intervention group (JD) will differ from a value chosen randomly from the control group (BL) [37]. We performed a post hoc sample size calculation using the primary endpoint of MAX, and using a 30% change in MAX, with the probability of a type 1 error (α = 0:05) and type 2 error (β = 0:2), the required sample size of n = 9 would be needed to yield a power of 0.80. Additionally, post hoc power analysis using the absolute values and standard deviation of the maximum rate of force development (RFD max ) in ND, with a probability of type 1 error (α = 0:05), and a sample size of n = 11, yielded a power of 88.7%. Data presented are the mean (standard deviation).

Results
There were twenty-one volunteer participants in total, with thirteen females and eight males. All participants were compliant with the performance of the modified EHGS and the use of the JD. The study subject characteristics are found in Table 1. The IPAQ physical activity categorical score was high for all seven ND, low in nine, and moderate in one of the ten T2D (Supplementary Table 1, found in Electronic Supplemental Material File, shows both categorical and continuous IPAQ variables, ESM 1_File).
Maximal peak force (MAX) at baseline in T2D was 273.6 (93.5) compared to 395.2 (142.6) N in ND (p < 0:05) Figure 2(a). JD did not affect MAX in either T2D or ND, and there was no difference between groups at any other time point. Based on Wang et al.'s normative data for age, gender, and weight, 2 subjects in the T2D were considered to have low MAX, [38] and 2 subjects in the T2D were considered probably sarcopenic based on the European Working Group on Sarcopenia in Older People (EWGSOP) criteria [7]. Analysis of the parameters of the modified EHGS curve showed that the rate of force development (Δforce/Δtime) at 25%, 75%, and 90% of the maximum force (RFD 25% , RFD 75% , and RFD 90%) and at maximum force (RFD max ) was not different between T2D and ND at baseline. JD did not modify RFD 25% or RFD 90% in either T2D or ND. In nondiabetic participants, JD increased RFD 75% after 7 days and Carryover and RFD max after 3 days, 7 days, and Carryover. In T2D participants, JD also increased RFD max after 3 days, 7 days, and Carryover (Table 2). Comparison between the two groups showed that ND had statistically significantly higher values of RFD 90% and RFD max at 7 days and Carryover compared to T2D ( Table 2).
There were no differences at baseline or any time points for force development times for t max , 90%, 75%, or 25% or fatigue resistance times FR 25 , FR 50 , and FR TF , between T2D and ND. In T2D, JD increased FR 25 and FR TF from baseline, after seven days, and three days after cessation (Carryover). In ND, JD increased FR 25 and FR TF from baseline after seven days. FR TF in T2D increased from baseline values by 50% and 53% after seven days and Carryover, respectively. Similarly, JD increased FR TF in ND by 50% after seven days ( Table 2).
The integrated area under the curve until task failure (iAUC TF ) was significantly different from BL values in both T2D and ND starting after 3 days of JD and until Carryover. JD increased iAUC TF in T2D by 40%, 95%, and 113% at JD3, JD7, and Carryover, respectively. In ND, JD increased iAUC TF by 83%, 130%, and 320% at JD3, JD7, and  Figure 1: Study protocol. Participants were asked to fast for 8 hr prior to the initial baseline modified explosive handgrip strength (EHGS) test. On the day of enrollment, participants were familiarized with the modified EHGS test by carrying out two practice sessions. During the visit, participants were instructed on the use of the jogging device (JD). Participants were asked to use JD a minimum of 3 times for 30 min per day for 7 days (JD1-7). On the evening of day 7, participants were asked to stop the use of JD and fast for 8 hrs. On day 10 (3 days after discontinuation of JD), a repeat EHGS was performed (Carryover). On each visit day, participants carried out a practice modified EHGS test, followed 1 hr later by duplicate EHGS test measurements. 4 BioMed Research International Carryover, respectively. iAUC TF was not significantly different between T2D and ND at any of the time points (

Discussion
The current study carried out in T2D and nondiabetic subjects showed that the maximal peak force (MAX) at baseline is significantly lower in T2D compared to ND. JD did not increase MAX in either group. Fatigue resistance time to task failure (FR TF ) was increased by JD in both T2D and ND after seven days. The area under the curve of force development until task failure (iAUC TF ) was also significantly larger compared to BL for both T2D and ND after seven days of JD and three days after discontinuation of JD. Analysis of the explosive part of the EHGS curve did not show differences in the rate of force development (RFD 25% , RFD 75% , RFD 90% , or RFD max ) at baseline between T2D and ND. There are no published data that examine RFD of the EHGS in diabetics. Investigators have shown that RFD is greater in younger females (age 20-27 yr) compared to older (70-90 yr) [33], and the aging process in females reduces RFD [26]. The effects of aging on muscle strength are well known [39,40]. The effect of JD on increasing RFD max in both ND and T2D was an unexpected finding, which requires further studies. Analysis of covariance with age or baseline RFD max values as covariates did not modify the significance of the findings. Demura et al. compared HGS to EHGS in young male volunteers and showed no difference in maximal grip strength between the two, with a strong correlation, suggesting that maximum peak force is likely very similar between EHGS and HGS [36]. There are conflicting data with regard to the association of handgrip strength (HGS) and T2D, with some studies suggesting that T2D has lower HGS compared to aged matched controls [5,41] and higher muscle strength, lowering the risk of developing T2D [42,43], while others refute such evidence [44,45]. Still, others have shown in an age-(59-60 yr) and gender-matched population of T2D and ND that while muscle strength in the upper body was similar among the groups, lower body muscle strength was significantly lower in T2D for both men and women [46]. A recent systematic review and meta-analysis of all

BioMed Research International
observational cohort studies suggest that increased HGS is associated with a lower risk of T2D, and HGS may be a risk indicator for T2D in the general population [22]. We examined the effects of age, gender, and T2D on MAX using the analysis of covariance on the observed significantly higher MAX at baseline in ND. We found that individually both T2D and gender were correlated with MAX, but age was not in this study data set (data found in Electronic Supplemental Material File, ESM 1_File). The latter could be due to the limited size of the data set. Others using much larger data sets have shown that grip strength is strongly correlated with age [39,47] and changes in maximum voluntary force decrease significantly after age 59 yr [40].
The repeated use of muscles provokes a reversible drop in performance referred to as muscle fatigue, which has been previously reviewed [48,49]. Similarly, the effects of exercise on muscle fatigue are also complex and have been previously reviewed [50]. In summary, failure of central and/or peripheral factors can contribute to muscle fatigue, making the latter difficult to simplify into a cause effects; thus, experimental approaches to measure task failure using one or two groups performing the same task (EHGS) before and after an intervention (JD) are valuable to study design [51].
Simple passive, nonexercise interventions to increase time to task failure are lacking. Barbosa et al. have used remote ischemic preconditioning (RIPC, exposure of a distal limb to five minutes of insufflation of a blood pressure cuff to 200 mmHg followed by 5 min of deflation for 3 cycles, to mimic ischemia-reperfusion) to decrease muscle fatigue. One session of RIPC increased the time to task failure by 11.2% [52]. In contrast to RIPC, JD does not produce ischemia to the extremities. The current study shows that JD performed for three days' increases task failure time (FR TF ) by 31% and 40% in ND and T2D, respectively. Our study is the first report of a passive device, which has been shown to minimally increase oxygen consumption above resting levels [29], to increase indices of fatigue resistance (FR 25 and FR TF ) in both T2D and ND. The latter also led to a significant increase in the total integrated area under the forcetime curve (iAUC TF ). Muscle strength is the best single measure of age-related muscle changes, including sarcopenia, and is associated with physical disability with reduced activities of daily living and functional limitations [53]. Evidence indicates that T2D patients have reduced muscle strength, and power [5,46], but its etiology remains to be better elucidated [54,55].
Parameter BL  JD3  JD7  Carryover  T2D  ND  T2D  ND  T2D  ND  T2D   Analysis of maximum force (MAX); rate of force development (RFD) at 90%,75%, and 25% of maximal force (RFD 90% , RFD 75% , and RFD 25% ); and maximum of rate development (RFD max , N/sec). Force development time (sec) for maximum and 90%, 75%, and 25% of the maximum force (t max , t 90 , t 75 , and t 25 ), fatigue resistance (sec) defined as the time at which the grip strength decreases to 50% and 25% of maximal force (FR 50 and FR 25 ), and fatigue resistance time to task failure (FR TF ). Integrated area under the curve for force vs. time in the entire curve until task failure (iAUC TF ). Mean and standard deviation (SD) for each. a T2D vs. ND; b BL vs. JD3, JD7, and Carryover in T2D; c BL vs. JD3, JD7, and Carryover in ND. All differences are at least p < 0:05.

6
BioMed Research International induced by eccentric exercise [63] and increase antioxidant expression [64]. Nitric oxide and reactive oxygen species (ROS) play an important and relevant role in skeletal muscle strength and fatigue resistance [65,66]. The former is in part modulated by the balance between S-nitrosylation and denitrosylation [67]. Additionally, augmentation of the nitric oxide cyclicguanosine monophosphate signaling has also been shown to reduce skeletal muscle fatigue [68].
There is strong evidence that ROS contributes to muscle fatigue. The mechanism of ROS action on skeletal muscle force and fatigue may involve the opening of the sarcoplasmic reticulum (SR) calcium release channel and inhibition of the calcium-dependent ATPase both, which increase calcium transients. Exaggerated ROS production during the early phases of fatiguing exercise causes loss of SR function, SR calcium leak, and a rise in intracellular calcium, decreasing force and increasing fatigue [65,69,70], and antioxidants have been shown to attenuate the latter [71,72]. Allen et al. have extensively reviewed the cellular mechanisms involved in skeletal muscle fatigue [49]. JD did not modify maximal peak force in this protocol; this is likely related to the mild severity of myopathy, if any, of the participants (only 2 of the 21 participants had below normal peak force at baseline). Additionally, five of the ten T2D participants were >60 yr of age, and only 2 of the 10 were considered sarcopenic. A recent review by Mesinovic et al. has highlighted the bidirectional relationship between T2DM and sarcopenia [12]. Using the predicate device WBPA for eight days, we have shown an increase in muscle strength (forelimb grip strength) in a rodent genetic model (mdx) of severely impaired muscle strength (Duchenne Muscular Dystrophy) [73]. In human volunteers, WBPA performed after exercise-induced muscle dysfunction, increased maximal voluntary contraction, accelerated muscle recovery, and decreased pain [74]. Finally, we observed a Carryover effect of JD on iAUC TF, indicating that the beneficial effects of JD may have either a genomic or posttranslational protein effect as its mechanism, similar to the findings observed for preconditioning cardioprotection with WBPA [75].
There are practical and clinical implications to this study. The JD is passive and simple to operate (push of the button) and can be used in both seated and supine postures, thus suitable for various populations, including those with limited cognitive and motor abilities. In the elderly, frail, and bedridden, passive motion has been shown to improve vascular function [76] and improves age-related reduction in vasodilatation, which is related to diminished NO bioavailability [77]. Physical activity is particularly important in the aged and frail and promotes carrying out activities of daily living [53]. In patients with spinal cord injury, passive limb movements have been shown to improve vascular health and tissue perfusion [78]. Walking as a physical activity intervention improves cardiovascular health [79][80][81][82], decreases hypertension [83], and decreases all-cause mortality [84]. In both critically ill intensive care unit survivors and noncritically ill hospitalized patients, physical activity enhances the recovery of functional exercise capacity and self-perceived functional status [85][86][87]. Moreover, a recent epidemiological study showed that daily walking alone is sufficient to reduce pneumonia-related mortality among older people who do not regularly engage in other exercise habits [88]. Physical activity and rehabilitation programs are widely recognized as a way to improve both general health and musculoskeletal status in patients with neuromuscular diseases, of which congenital myopathy is one of them [89]. In animal models of congenital myopathy (Duchenne Muscular Dystrophy, mdx), WBPA has been shown to improve skeletal muscle strength, reduce muscle damage and inflammatory phenotype, and improve myocardial function [73,90]. In the general population and specifically those with T2D, JD would be an important adjunct to the current recommendations of diet and exercise, as JD is able to reduce glycemic spikes and improve glucose homeostasis, while improving the time to task failure as a measure of endurance. JD can be an adjunct to physical therapy, providing additional physical activity interventions as an inpatient and outpatient without the need for hands-on supervision. In athletes pre-and post-conditioning using JD, similar to the predicate device, WBPA also has the potential to reduce delayed onset muscle soreness and improve endurance [63,74]. The small footprint of the device allows the use of the device under a desk for those whose work requires prolonged sitting time [91,92]. JD improves time to task failure, in addition to the previously reported effects on the reduction of sedentary induced hypertension, improved glucose homeostasis, and improved heart rate variability, and these effects have clear health benefits.
There are limitations to the present study that must be acknowledged. This study was part of a larger study on the effects of JD on glucose homeostasis, previously reported [15]; therefore, parameters of the modified EHGS were not the primary endpoints of the protocol, and thus, the study design lacked controls such as sedentary time controls, active weight bearing activity (10,000 steps training), and age and BMI. Each subject's baseline measurements served as his or her own control. Furthermore, the study was designed as a noninvasive study; thus, blood sampling or muscle biopsies were not performed, which would have provided additional mechanistic information. We also did not measure the long-term effects of JD on EHGS. This study was also not designed to specifically determine the mechanisms by which JD improves time to task failure. Familiarization with EHGS procedures over time could potentially account for the observed effects of JD. The latter is unlikely, since each participant underwent two practice sessions prior to starting the study, and one practice session prior to each measurement; thus, EHGS was performed at least seven times prior to JD3. Based on our previous work, however, it is more reasonable to suggest that nitric oxide (eNOS and/or nNOS) as well as antioxidants may in part be responsible for the effects reported. Taken together with our previous studies on JD, the present results provide a compelling rationale for early adoption of this noninvasive, nonpharmacologic intervention.
Notwithstanding the limitations and in addition to the previously reported beneficial effects, JD performed for at 7 BioMed Research International least seven days prior to EHGS increased time to task failure and iAUC TF of the force-time curve; the latter occurred in both T2D and ND. Given the portability and ease of use of JD, the latter is a reasonable, simple intervention to decrease sedentary behavior and improve muscle fatigue resistance under various clinical and nonclinical scenarios.

Data Availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Ethical Approval
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. The study and its informed consent were approved by the Western Institutional Review Board (WIRB) (WIRB, Puyallup, WA 98374-2115) on April 2018 (WIRB No. 1184829).

Consent
Informed consent was obtained from all individual participants included in the study.

Conflicts of Interest
JAA performs research for Sackner Wellness Products LLC and is a US copatent holder for Gentle Jogger, the Passive Simulated Jogging Device. JRL is a research scientist consultant to Sackner Wellness Products LLC. VB is a part-time study coordinator and employee of Sackner Wellness Products LLC. MAS is the president of Sackner Wellness Products LLC and is a US copatent holder for Gentle Jogger Passive Simulated Jogging Device (deceased).

Authors' Contributions
JAA is responsible for study design, data analysis, and writing of the manuscript. JRL is responsible for data acquisition, data analysis, and writing of the manuscript. VB is responsible for all study subject data files and data acquisition and writing of the manuscript after the 1st draft. MAS is responsible for study design, data analysis, and writing of the manuscript. Jose A Adams, Jose R Lopez, Veronica Banderas, and Marvin A Sackner contributed equally to this work. Marvin A Sackner is deceased. All authors have reviewed the data and the manuscript and endorse its conclusion and consent to its publication.

Acknowledgments
Sackner Wellness Products LLC funded this study. We acknowledge the scientific contribution in this manuscript and in science of Professor Marvin A. Sackner (RIP). His valuable contribution to science and medicine during his many years of teaching and research has led to remarkable breakthroughs for the betterment of society. His tireless work in the field of cardiopulmonary research, his mentorship to so many, and his friendship have clearly left a legacy. The authors also gratefully acknowledge Christina Staalstrom for her help with participant recruitment.

Supplementary Materials
ESM_File_S1: CONSORT checklist, study diagram, and supplementary Tables. This file contains the Consolidated Standards of Reporting Trials (CONSORT) checklist, study diagram, and supplementary Tables 1 and 2 as well as statistical analysis tables. Table 1 provides the IPAQ short form variables for each participant. Table 2 shows Common Language Effect Size (CLES) of JD in T2D and ND for the significant variables. Figure 1S contains rate of maximal force development (RFDmax) in T2D and ND at baseline (BL), three and seven days of JD (JD3 and JD7), and 3 days after discontinuation of JD (Carryover). The section on statistical data analysis contains statistical tables for ANCOVA performed with maximum rate of force development (RFD max ) and peak force (MAX) as dependent variables, on days of testing, age, gender, and diabetes as covariates. (Supplementary Materials)