Phytochemical Study and In Vitro Biological Activities of Hibiscus panduriformis Burm. f. (Malvaceae), Alternanthera pungens Kunth (Amaranthaceae), and Wissadula rostrata (Schumach.) Hook. f. (Malvaceae)

The present study investigated the phytochemical content of Hibiscus panduriformis, Alternanthera pungens, and Wissadula rostrata and assessed their radical scavenging and anti-inflammatory properties. n-Hexane, dichloromethane (DCM), ethyl acetate, and methanol extracts were prepared from the powdered plant parts. The phytochemical analysis was performed using qualitative high-performance thin-layer chromatography, and polyphenols were quantified using well-established methods. The anti-inflammatory effect was by lipoxygenase inhibition, while the antiradical impact was evaluated through DPPH and ABTS radicals. Steroids, triterpenoids, flavonoids, and tannins were identified in the three plants. The highest phenolic content (95.67 ± 2.19 mg gallic acid equivalent/g) was obtained in the methanolic extract of W. rostrata, while the lowest was measured in H. panduriformis. H. panduriformis was found to be highly rich in flavonoids (61.22 ± 0.09 mg rutin equivalent/g), condensed tannins (62.53 ± 0.03 mg catechin equivalent/g), and hydrolyzable tannins (125.1 ± 1.02 mg tannic acid equivalent/g). The methanolic extract of H. panduriformis displayed the greatest antilipoxygenase activity with an IC50 value of 8.78 ± 1.05 μg/mL. It should be noted that although a moderate to low effect was observed, the extracts were more likely to scavenge DPPH (IC50 values ranged from 0.106 ± 0.010 to 1 mg/mL) than ABTS radicals. There was a strong to moderate correlation between the antilipoxygenase and DPPH radical scavenging effects of the methanolic extracts and total phenolic content (antilipoxygenase, r = 0.7175; DPPH, r = 0.9376). Furthermore, it is worth noting that this is the first report investigating the phytochemical analysis and in vitro biological properties of Hibiscus panduriformis. The results highlighted the richness of this plant in polyphenols and demonstrated its high and moderate effects on lipoxygenase and DPPH radicals, respectively. To this intent, further in vivo and in vitro studies on this plant, along with exhaustive phytochemical analysis, are needed.


Introduction
The inflammation process results from a response to noxious stimuli, including microorganisms, toxic compounds, or injured cells [1].Inflammation is, therefore, a defense mech-anism used by the organism to remove harmful stimuli and stimulate tissue repair [1,2].Acute inflammatory response usually lasts for a few days and is obtained when the inflammatory triggers are eliminated with less tissue damage.Inflammation becomes chronic more frequently after the failure to eradicate foreign bodies during acute inflammation, leading to various chronic inflammatory diseases such as diabetes, cardiovascular diseases, cancer, and autoimmune diseases [1,3,4].Inflammation triggers multiple signaling pathways leading to the stimulation and release of several inflammatory mediators, including cytokines, proteases, prostaglandins, and leukotrienes [1].
Furthermore, other important inflammation markers involve free radicals or reactive oxygen species (ROS) [1].The primary role of ROS production by polymorphonuclear cells at the site of inflammation is to kill pathogens [5].However, chronic inflammation promotes excessive ROS production, leading to oxidative stress because of an imbalance between ROS production and elimination by the body's antioxidant system [5][6][7].
Steroidal and nonsteroidal anti-inflammatory drugs are usually synthetic compounds used to fight or reduce inflammation in inflammation-related diseases [8].However, using such drugs is linked to several adverse effects, leading most people to use medicinal plants to cure inflammationassociated diseases [8].
In most developing and developed countries, there is an enormous surge in the use of herbal medicine for disease prevention and treatment.Herbal-based drugs or medicinal plants are believed to promote healthier living than synthetic drugs [9].
Alternanthera pungens (A.pungens) Kunth (Amaranthaceae), Wissadula rostrata (W.rostrata) (Schumach.)Hook.f. (Malvaceae), and Hibiscus panduriformis (H.panduriformis) Burm.f. (Malvaceae) are three plants used against several diseases in Burkina Faso.Alternanthera pungens (Syn.Alternanthera repens O. Ktze.) is a creeping perennial herbaceous plant with a 10-15 cm long stem with hair [10,11].Like most Alternanthera species, A. pungens is traditionally used to treat inflammation, pain, infectious diseases, and gastric disorders [10,11].Wissadula rostrata (Syn.Wissadula amplissima (L.) R.E.Fr.var.rostrata (Schumach.& Thonn.)) is a perennial shrub found near inhabited places.In traditional medicine, W. rostrata is commonly used against insect bites, diarrhea, inflammation, and allergy [11,12].In Burkina Faso traditional medicine, parts for therapeutic use include leafy stems and buds for A. pungens.In contrast, roots, leaves, and flowers administered externally or internally were used for W. amplissima [11].Contrary to these two plants, few studies have been dedicated to Hibiscus panduriformis (Malvaceae).Although various authors have studied the genus Hibiscus and reported its use in traditional medicine, there are practically no reports regarding the traditional use of H. panduriformis.According to the "Useful Tropical Plants Database," Hibiscus panduriformis is an upright and branched subwoody plant, annual to perennial, of short lifespan, reaching up to 3 m high with stems, branches, and leaves velvety-tomentose [13].The plant is mainly used as a fiber and ornamental source, and its flowers are eaten in Congo [13].In South India, the leaves of Hibiscus panduriformis are used to repair bone fractures [14].
The present study was undertaken to assess the phytochemical analysis, radical scavenging, and lipoxygenase inhibitory activities of the three medicinal plants.
The spectrophotometric measurements were carried out using a Shimadzu UV-Vis Spectrophotometer and microplate spectrophotometer (BioTek Epoch, USA).Highperformance thin-layer chromatography (HPTLC) silica gel F 254 plates (20 cm × 10 cm) from Merck (Darmstadt, Germany) were used to identify the main phytoconstituents from the plant's extracts.

Collection and Extraction of the
Plant's Materials.The leafy stems of Alternanthera pungens, Wissadula rostrata, and Hibiscus panduriformis were collected from the periurban area around Ouagadougou, the capital city of Burkina Faso (Figure 1).
Each plant was taxonomically identified, and the voucher specimens were deposited in two different herbariums.Wissadula rostrata and Alternanthera pungens voucher specimens were deposited in the national herbarium of University Joseph KI-ZERBO under numbers 17259 and 17260, respectively.The Hibiscus panduriformis voucher specimen was deposited in the herbarium of the Centre National de Semences Forestières (CNSF, Burkina Faso) under reference number CNSF-1400.
The leafy stems of the plants were shade-dried at room temperature.Once dry, after two weeks, the plant's materials were powdered using a mechanic grinder.Extraction was done sequentially using solvents of increasing polarity, from n-hexane, dichloromethane (DCM), and ethyl acetate to methanol.The residue obtained in a previous extraction step was left to dry and extracted with successive solvents.Briefly, 100 g of each plant powder was first macerated with 500 mL of n-hexane under shaking for 24 hours.The extract was filtered, and the residue was resuspended in 500 mL of n-hexane for another 24 hours.Finally, this extraction is repeated once using the same solvent and following the same procedure.The n-hexane filtrates (to a volume approximately equal to 1.4 L) are mixed, and the residue is allowed to dry at room temperature.The different filtrates (n-hexane, DCM, ethyl acetate, and methanol (slightly acidified)) were concentrated using a rotary evaporator at 40 °C.Each plant's hexane, DCM, ethyl acetate, and methanol extracts were conserved at 4 °C for further analysis.BioMed Research International identify steroids, triterpenoids, flavonoids, and tannins [15].20 μL (6 mg/mL) of samples was applied to the plate as 5 mm band length using the CAMAG Linomat 5 TLC sample applicator (CAMAG, Muttenz, Switzerland) equipped with a syringe.After application, the plates were developed vertically, ascending in a glass twin-trough chamber presaturated for 30 minutes with an adequate mobile phase.The chromatographic run length was 70 mm from the bottom edge of the plate.Following the development, the plates were dried using a hairdryer, and specific reagents were used to reveal the phytoconstituents.Flavonoids were detected with Neu's reagent; tannins were shown using a FeCl 3 2% reagent.To observe steroids and triterpenes, the plates were sprayed with the Liebermann-Burchard reagent.The plates were air-dried and heated at 110 °C, except for those used to detect tannins, which were heated at 100 °C [15].

Estimation of Total Phenolic Content (TPC)
. The total polyphenol content was quantified spectrophotometrically using the Folin-Ciocalteu reagent (FCR) [16] with slight modifications.A calibration curve was realized with gallic acid concentrations ranging from 0.0001 to 0.5 mg/mL.The reaction mixture was prepared with 1 mL of plant extract solution (prepared from a stock solution of 1 mg/ mL) and 1 mL of FCR (0.2 M).After 8 minutes at room temperature, 3 mL of 7.5% (w/v) sodium carbonate was added.
The mixture was allowed to incubate for 30 min at room temperature, and the absorbance was read at 760 nm in a spectrophotometer (Shimadzu UV-Vis, Japan) against a blank sample (the extract was replaced by distilled water).Total phenolic content (TPC), expressed as milligram gallic acid equivalents per gram of dry extract weight (mg GAE/g), was calculated from the gallic acid calibration curve (y = 10 459x + 0 0335, R 2 = 0 9993).The measurements were conducted in triplicate.

Estimation of Total Flavonoid Content (TFC).
The total flavonoid content (TFC) was determined using the aluminum chloride (AlCl 3 ) colorimetric assay [17] with slight modifications.Briefly, 1 mL of methanolic extract of 1 mg/ mL stock solution was mixed with 0.3 mL NaNO 2 (5%).Then, 0.3 mL of 10% AlCl 3 methanolic solution was added.Six minutes later, 2 mL NaOH (1 M) was added, and the total volume was made up to 5 mL with 2.4 mL of distilled water.The reaction mixture was left to stand at room temperature for 30 min, after which the absorbance was read at 510 nm with a Shimadzu UV-Vis (Japan) against a blank.Rutin was used as a standard for the calibration curve (y = 2 5608x + 0 0034, R 2 = 0 9995), and total flavonoid content (TFC) was expressed as milligram rutin equivalents (mg RuE) per gram of dry extract weight of three independent experiments.

Determination of Condensed Tannin Content (CTC).
The vanillin/hydrochloric acid method was used to assess the condensed tannin content [18] in methanolic extracts of the three plants.Diluted solutions of plant extracts or catechin, the reference compound, were made from a 1 mg/mL stock solution.In cleaned glass tubes protected from light with aluminum foil, 0.5 mL of each sample (extract/catechin), 3 mL of a 4% methanolic vanillin solution, and 1.5 mL of concentrated hydrochloric acid were thoroughly mixed.Tubes were allowed to stand at room temperature for 20 min, and absorbances were recorded at 500 nm 3 BioMed Research International (Shimadzu UV-Vis, Japan) against a blank.The content of condensed tannin was estimated from the catechin calibration curve (y = 2 6248x + 0 0266, R 2 = 0 9962) and expressed as milligram catechin equivalents (mg CE) per gram of dry extract weight.

Determination of the Hydrolyzable Tannin Content (HTC).
Hydrolyzable tannins are a group of natural polyphenols with a central core of glucose or another polyol being esterified with either gallic acid (the compounds are called gallotannins) or hexahydroxydiphenic acid (also called ellagitannins) [19].The hydrolyzable tannin content of the methanolic extracts was determined using tannic acid as the reference compound for the calibration curve [20].Diluted methanolic extracts and tannic acid were prepared from stock solutions (extract/standard) of 1 mg/mL. 1 mL of each solution was mixed with 5 mL of 2.5% KIO 3 .The mixture was vortexed and left to incubate for 4 minutes at room temperature.After that, the absorbance of the redcolored mixture was read at 550 nm (Shimadzu UV-Vis, Japan) against a distilled water blank.The hydrolyzable tannins were quantified from the tannic acid calibration curve (y = 0 4055x + 0 0213, R 2 = 0 9973), and the results were expressed as milligram tannic acid equivalents per gram of dry weight (mg TAE/g).

Lipoxygenase (LOX) Inhibition
Assay.The lipoxygenase inhibition assay was performed on a 96-well microplate with linoleic acid as substrate [21].The test sample was dissolved in DMSO at 8 mg/mL, and diluted solutions were prepared.3.75 μL of each diluted solution was added to the reaction mixture containing 146.25 μL of an aqueous solution of lipoxygenase (820.51U/mL).The plate was allowed to incubate for three minutes at room temperature, and the reaction was initiated by adding 150 μL of linoleic acid solution (1.25 mM).An enzyme blank without inhibitor was made of 153.75 μL of borate buffer (0.2 M, pH = 9) and 146.25 μL of an aqueous solution of lipoxygenase (820.51U/mL).The sample blank (150 μL of borate buffer + 3 75 μL of the test sample and 146 25 μL of enzyme) was also realized.Zileuton was used as a reference compound.Inhibitory activity was measured in triplicate using a microplate spectrophotometer (BioTek Epoch, USA) at 234 nm.The percent of lipoxygenase inhibition was obtained from the following equation: where A C is the absorbance of the enzyme-absorbance of enzyme blank-and A S is the absorbance of the sampleabsorbance of the sample blank.
2.9.Determination of the 2,2-DPPH Radical Scavenging Activity.The 2,2-DPPH • radical scavenging activity was assessed using a Shimadzu UV-Vis Spectrophotometer [22].Trolox and plant extracts were dissolved in methanol to yield a final 1 mg/mL concentration, from which diluted solutions were prepared.1 mL of sample or reference compound methanolic solution was mixed with 4 mL of 0.004% methanolic DPPH work solution.The mixture was incubated in the dark at room temperature for 10 minutes.
For the blank sample, extract/Trolox was replaced with 1 mL of methanol.Absorbances were recorded at 517 nm in a Shimadzu UV-Vis Spectrophotometer (Japan), and measurements were conducted in triplicate.The DPPH radical scavenging activity (%) was calculated as follows: where A B and A S represent, respectively, the absorbances of the blank and sample/Trolox.The IC 50 (mg/mL), sample concentration or standard compound that produces half-maximal inhibition, and antioxidant radical power (ARP) were determined.Usually, a lower IC 50 value indicates a high radical scavenging effect [23].
Assay.The ABTS •+ was generated by reacting the ABTS (7 mM) salt with potassium persulfate (39.2 mM) according to the method of Re et al. [24].The mixture was incubated in the dark for 12-16 hours at room temperature.Before use, the stock solution was diluted to obtain an absorbance equal to 0.7 at 734 nm.Diluted solutions were prepared from a stock solution (plant sample) of 4 mg/mL. 1 mL of the different solutions was added to 4 mL of the diluted ABTS •+ solution, and absorbances were read at 734 nm in a spectrophotometer (Shimadzu UV-Vis, Japan) after 10 minutes of incubation in the dark.

ABTS scavenging activity
where A C and A S are, respectively, the absorbances of the control (ABTS radical solution without extract or Trolox) and sample/Trolox (ABTS radical + extract/Trolox).
As in the DPPH assay, IC 50 (milligrams per milliliter) values estimated the ABTS radical scavenging effect.
2.11.Statistical Analysis.The analyses were conducted in triplicate, and the results were expressed as means ± standard deviation (SD).Pearson's coefficients were determined for the correlation study.As previously reported, the GraphPad Prism (version 8.0) was used to perform the statistical differences between the extracts and to compare the extracts to the control, using a two-way analysis of variance (ANOVA) followed by multiple comparison tests [25].The difference was considered statistically significant for a p value < 0.05.

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BioMed Research International
The appearance of red-violet, blue, and green grass spots (Figure 2) revealed the presence of triterpenoids and steroids [26], respectively.
The HPTLC fingerprint (Figure 3) of flavonoids was obtained after development in the solvent system ethyl ace-tate/formic acid/water (80/10/10, v/v/v) for both the ethyl acetate and methanol extracts from the three plants.The ethyl acetate/formic acid/water (80/10/10, v/v/v) system appears more suitable for separating flavonoid compounds in the methanol extracts than the ethyl acetate extracts of the three plants.
Dark blue and greenish-black colors after derivatization at visible light highlight the presence of tannins (Figure 4) in the ethyl acetate and methanol extracts.The plates were developed in a chamber containing the following mobile phase solvent system: ethyl acetate-methanol-water-chloroform (18-2.4-2.1-6,v/v/v/v).Tannins were observed by spraying the plates with a 2% FeCl 3 solution.
3.3.Lipoxygenase (LOX) Inhibitory Activity.The results of lipoxygenase inhibitory activity by the organic solvent extracts are presented in Table 2.
The organic solvent extracts were highly statistically different (p < 0 0001).Comparatively to zileuton (p < 0 0001), the extracts from H. panduriformis, A. pungens, and W. rostrata showed high to weak effects with IC 50 values ranging from 8.78 to 194.88 μg/mL.The methanolic extract of Hibiscus panduriformis, with an IC 50 value of 8 78 ± 1 05 μg/mL, demonstrated good inhibitory activity compared to the other organic solvent extracts.With IC 50 values greater than 200 μg/mL, the hexane extract of H. panduriformis and methanolic extracts of both A. pungens and W. rostrata did not exhibit significant inhibitory activity.The inhibition percentages at this concentration (200 μg/mL) were 47 90 ± 0 90, 49 27 ± 0 87, and 33 14 ± 1 54 μg/mL, respectively, for the hexane extract of H. panduriformis and methanolic extracts of A. pungens and W. rostrata.

DPPH and ABTS Radical Scavenging
Assay.The free radical scavenging ability of the organic solvent extracts was assessed using the DPPH and ABTS radical scavenging assays.The results are shown in Tables 3 and 4. The extracts were statistically highly (p < 0 0001) different regarding the two assays.
Overall, the results showed a weak radical scavenging effect of the different extracts on the two free radicals.The extracts were more active against DPPH than ABTS radicals.Trolox was at least thirty and five hundred times more potent than most extracts against DPPH and ABTS radicals, respectively (Table 3).The three plants' extracts, particularly the ethyl acetate and methanol extracts, were more effective on the DPPH radicals.In the two free radical scavenging tests and for each plant, there was a highly significant difference (p < 0 0001) between the different organic solvent extracts.

Correlation Studies.
To find an eventual correlation between the three plants' in vitro biological properties and total polyphenol compounds, the Pearson correlation coefficient (r) and coefficient of determination (R) were obtained from GraphPad Prism.Some of these results are reported in Table 5.
A correlation coefficient equal to 0 means no linear relationship, while a negative or positive value indicates a Values presented are the average of three experiments ± standard deviation. 1, 2, 3, 4Expressed in terms of GAE, RuE, CE, and TAE (milligram of gallic acid, rutin, catechin, and tannic acid equivalents per gram of dry extract, respectively).In each row, values bearing different letters (a, b, and c) are considered highly statistically significant at p < 0 0001 (two-way ANOVA followed by Tukey's test).Values are presented as mean ± SD; each extract was tested in triplicate.For each plant, extracts were compared regarding the organic solvent (two-way ANOVA followed by Tukey's test) and zileuton (two-way ANOVA followed by Dunnett's test).Within each column, values bearing different letters (a, b, c, d, e, f, g, h, i, j, and k) are considered highly statistically significant at p < 0 0001.All organic extracts were statistically significantly (p < 0 0001) different from zileuton.
6 BioMed Research International negative or positive linear correlation.When the coefficient value is between 0 and 0.3, 0.3 and 0.7, and 0.7 and 1, it means a weak, moderate, and strong correlation, respectively [25].Based on these interpretations, it can be suggested that the DPPH radical scavenging effect is strongly positively correlated with TPC and strongly negatively correlated with the TFC, CTC, and HTC.Total phenolic content is moderately correlated (r = 0 6636) with the ABTS radical scavenging activity.There was a positive (0 < r ≤ 0 3) and a negative (−0 3 < r ≤ 0) weak correlation between ABTS radical scavenging effect and TFC (r = 0 2805), CTC (r = 0 0563), and HTC (r = −0 1489).The correlation study found a weak positive correlation (r = 0 3621) between the two radical scavenging methods.
Pearson's correlation coefficient between the antilipoxygenase activity and total phenolic content showed a weak (r = 0 2016) to moderate (r = 0 7175) correlation regarding the ethyl acetate and methanolic extracts.There were no significant correlations between TPC and the antilipoxygenase activity of the n-hexane (r = −0 4923) and DCM (r = −0 1348) extracts.Strong positive and moderate positive correlations were observed between the antilipoxygenase of the methanolic (r = 0 9149) and ethyl acetate (r = 0 5295) extracts and the DPPH radical scavenging effect.However, Pearson's correlation coefficient was weakly negative (r = −0 0449) between the ABTS assay and the antilipoxygenase of the methanolic extracts.

Discussion
Various medicinal plants have been used in folk medicine for their anti-inflammatory properties.Numerous in vitro and in vivo assays have been developed to assess the antiinflammatory effect of plant samples [27].Moreover, studies have reported the role of reactive oxygen species (ROS) in inflammation; therefore, scavenging ROS may increase the therapeutic strategy for inflammatory diseases [27].The present study reports the lipoxygenase inhibitory activity, radical scavenging effect, and phytochemical analysis of H. panduriformis, A. pungens, and W. rostrata.
The phytochemical study has revealed the presence of steroids, triterpenes, and polyphenol compounds in the extracts of the three plants.Quantification of the polyphenolic compounds revealed that (i) phenolics were abundant in W. rostrata and (ii) flavonoids and condensed and hydrolyzable tannins were found to be rich in H. panduriformis.The results are consistent with previous reports [12,28] regarding the total phenol content in W. rostrata.Polyphenol compounds present in Alternanthera pungens were mostly phenolic compounds, confirming other studies [10,29].Globally, the results indicate that the three plants have a high content of polyphenol compounds.
Lipoxygenases are oxidative enzymes that regulate inflammatory responses by generating proinflammatory or anti-inflammatory mediators known as leukotrienes and lipoxins [30,31].Based on their ability to incorporate oxygen atoms into arachidonic acid, LOXs are divided into four subtypes, including 5-LOX, 8-LOX, 12-LOX, and 15-LOX [32].5-LOX, 12-LOX, and 15-LOX have been implicated in several inflammation-related disorders such as atherosclerosis, asthma, and cancer [32].Consequently, inhibiting LOX activity is crucial in the therapeutic strategy to alleviate  Values are presented as mean ± SD, and each extract was tested in triplicate.
For each test (DPPH and ABTS) and each plant, extracts were compared regarding the organic solvent (two-way ANOVA followed by Tukey's test) and Trolox (two-way ANOVA followed by Dunnett's test).For the DPPH test, values bearing different letters are considered highly statistically significant at p < 0 0001 (a vs. b, a vs. c, a vs. d, a vs. e, a vs. f, a vs. .For the ABTS test, values bearing different letters are considered highly statistically significant at p < 0 0001 (a vs. b, a vs. c, a vs. d, a vs. e, b vs. e, c vs. d, c vs. e, and d vs. e) , at p = 0 0053 (b vs. c) , and at p = 0 0165 (b vs. d) .H. pan: Hibiscus panduriformis; A. pun: Alternanthera pungens; W. ros: Wissadula rostrata; Hex: hexane extract; DCM: dichloromethane extract; EtOAc: ethyl acetate extract; MeOH: methanol extract.BioMed Research International these inflammatory disorders.The anti-inflammatory effect of the three plants was obtained through the in vitro inhibition of soybean 15-lipoxygenase.The results in Table 1 indicate differences in their modes of LOX inhibition.The extracts may be classified as low, moderate, and highly active.n-Hexane extract of H. panduriformis, methanolic extracts of A. pungens and W. rostrata, DCM and ethyl acetate extracts of A. pungens showed weak to insignificant activity (IC 50 > 100 μg/mL) with respect to LOX inhibitory activity.n-Hexane extracts of A. pungens and W. rostrata and DCM and ethyl acetate extracts of H. panduriformis and W. rostrata exhibited moderate LOX inhibitory effect (10 μg/mL ≤ IC 50 < 100 μg/mL).The methanolic extract of H. panduriformis can be classified as highly active against lipoxygenase (IC 50 < 10 μg/mL).Among the investigated extracts of each plant, the best LOX inhibition was obtained with the methanolic extract of H. panduriformis, n-Hexane extract of A. pungens, and DCM extract of W. rostrata.The results are consistent with other authors that have reported the anti-inflammatory activity, especially the antilipoxygenase activity of the Malvaceae family [28,33,34] and some species from the genus Alternanthera (Amaranthaceae) [10].For instance, the ethanolic, hydroacetonic, and aqueous extracts of W. amplissima also developed a moderate (IC 50 < 100 μg/mL) inhibitory activity of lipoxygenase enzyme [28].To the best of our knowledge, this is the first report about the antilipoxygenase activity of Hibiscus panduriformis and Alternanthera pungens.Medicinal plants contain numerous phytoconstituents that can be responsible for the good inhibitory effect of the three plant extracts.The phytochemical analysis has revealed the presence of steroids, triterpenes, and polyphenol compounds in the extracts of the three plants.These phytochemicals are known to elicit anti-inflammatory effects, primarily through the inhibition of lipoxygenase [26,35,36].Pearson's correlation coefficient was used to find an eventual correlation between the antiinflammatory activity and polyphenol compound contents.The results indicate that besides these compounds, other phytochemical compounds such as triterpenes and steroids probably contribute to the lipoxygenase inhibitory potential of the plants.
Recent evidence has stated the crucial role of reactive oxygen species (ROS) in the initiation, progression, and resolution of inflammation [37,38].Scavenging ROS contributes, therefore, to eliminate inflammation.The radical scavenging capacity of the extracts was evaluated using the radicals DPPH and ABTS.The results showed a low radical scavenging effect on the two radicals compared to Trolox.
The methanolic extract of H. panduriformis gave higher DPPH scavenging activity than that of other organic solvent extracts.Although there are no reports on the pharmacological investigations of H. panduriformis, antioxidant activity has been reported on other Hibiscus species [39,40].Although weak, the DPPH scavenging effect of the ethyl acetate and methanolic extracts of A. pungens and W. rostrata was comparable to that of other previous reports [12,28,29].The DPPH radical scavenging activity could have been underestimated.Various parameters, including DPPH concentration, plant extract composition, and incubation time, are involved in the DPPH radical scavenging activity [41].In that study, DPPH was incubated with the extract for 10 minutes, while the literature reports an incubation time between 15 and 60 minutes.Only the methanolic extracts of the three plants exhibited a scavenging effect on the ABTS radical.However, this result was at least four hundred times less potent than Trolox.
It is well known that polyphenols are closely associated with antioxidant and anti-inflammatory activities of different medicinal plant parts [42,43].The weak positive correlation (r = 0 3621) between the DPPH and ABTS assays indicates differences in antiradical capabilities.The different extracts were likely more effective against DPPH radicals than ABTS ones (Tables 2 and 3), and the correlation studies revealed that the total phenolics contribute highly and moderately to the antiradical capacity of the three plants.However, based on earlier studies [43], this result does not mean that other polyphenol compounds, such as flavonoids and tannins, are not involved in the total free radical scavenging effect.Indeed, the antioxidant activity depends mainly on the structure and concentration of phenolic compounds, and polyphenol compounds can act (i) synergistically, (ii) additively, or (iii) antagonistically to eliminate free radicals [43].Based on the correlation study between the anti-inflammatory effect and DPPH radical scavenging activity, it can be noticed that scavenging free radicals is involved in the anti-inflammatory property of the different extracts.Taken together, the results suggest that besides other phytochemicals, total phenolics are high contributors to the three plants' radical scavenging and antilipoxygenase properties.

Conclusions
The present study stated the phytochemical search, antilipoxygenase, and radical scavenging activities of Hibiscus panduriformis, Alternanthera pungens, and Wissadula rostrata.

Figure 1 :
Figure 1: Location map showing the collection area of the plants.

Figure 2 :
Figure 2: HPTLC profile for steroids and triterpenoids detected in the hexane and dichloromethane (DCM) extracts of (A) Hibiscus panduriformis, (B) Alternanthera pungens, and (C) Wissadula rostrata.The plates were developed into a chamber containing the mobile phase solvent system n-hexane/ethyl acetate (20 : 4, v/v).

Figure 3 :
Figure 3: HPTLC chromatogram for flavonoids detected in the ethyl acetate and methanol extracts of (A) Hibiscus panduriformis, (B) Alternanthera pungens, and (C) Wissadula rostrata.The plates sprayed with the Neu reagent gave spots with orange, yellow, blue, and green colors, indicating the presence of flavonoids.
g, b vs. c, b vs. d, b vs. e, b vs. f, b vs. g, c vs. d, c vs. e, c vs. f, c vs. g, d vs. e, d vs. f, d vs. g, e vs. f, e vs. g, and f vs. g)

Table 1 :
Polyphenol compound contents of the methanol extracts of the three plants.

Table 2 :
IC 50 (μg/mL) values of lipoxygenase (LOX) activity inhibition from the organic solvent extracts of the three plants.

Table 4 :
Antiradical power (ARP) values of the DPPH and ABTS radical scavenging tests of different organic extracts of H. panduriformis, A. pungens, and W. rostrata.

Table 3 :
DPPH and ABTS radical scavenging effect of different organic extracts of H. panduriformis, A. pungens, and W. rostrata.

Table 5 :
8orrelations of IC 50 values of DPPH and ABTS radical scavenging activity of the methanolic extracts of the three plants with total polyphenolic compounds.: correlation coefficient; R 2 : coefficient of determination.There was no statistically significant (p > 0 05) difference between the correlations.TPC: total phenolic content; TFC: total flavonoid content; CTC: condensed tannin content; HTC: hydrolyzable tannin content.8BioMedResearch International Findings revealed that total phenolics are mainly responsible for these activities.To the best our knowledge, this is the first report on the antilipoxygenase and radical scavenging of H. panduriformis.Further studies are needed to identify the polyphenols present in the extracts, mostly in H. panduriformis. r