Computational Methods for Automated Analysis of Malaria Parasite Using Blood Smear Images: Recent Advances

Malaria comes under one of the dangerous diseases in many countries. It is the primary reason for most of the causalities across the world. It is presently rated as a significant cause of the high mortality rate worldwide compared with other diseases that can be reduced significantly by its earlier detection. Therefore, to facilitate the early detection/diagnosis of malaria to reduce the mortality rate, an automated computational method is required with a high accuracy rate. This study is a solid starting point for researchers who want to look into automated blood smear analysis to detect malaria. In this paper, a comprehensive review of different computer-assisted techniques has been outlined as follows: (i) acquisition of image dataset, (ii) preprocessing, (iii) segmentation of RBC, and (iv) feature extraction and selection, and (v) classification for the detection of malaria parasites using blood smear images. This study will be helpful for: (i) researchers can inspect and improve the existing computational methods for early diagnosis of malaria with a high accuracy rate that may further reduce the interobserver and intra-observer variations; (ii) microbiologists to take the second opinion from the automated computational methods for effective diagnosis of malaria; and (iii) finally, several issues remain addressed, and future work has also been discussed in this work.


Introduction
Malaria has turned into a major risk to individuals worldwide as one of the main reasons for causalities across the world. It is a curable infectious disease caused by a protozoan parasite that can be life-threatening. As per the latest report of the World Health Organization (WHO), in 2019, 229 million malaria cases were detected worldwide, and causalities were reached to 409000. In 2018, 228 million malaria cases were detected, and causalities were reached 411000 [1].
In 2016 and 2017, about 1.09 million and 0.84 million malaria cases were registered in India, in which most of the malaria cases were P. falciparum species affected [2].
Dr. Ronald Ross first discovered malaria transmission in the human body by mosquitoes in 1897 [3]. e main reason for malaria is a protozoan parasite. e plasmodium genus infects the red blood cells (RBC) of the human body, which causes malaria [4]. In general, female Anopheles mosquitoes and human beings are the two main hosts infected by the parasite. When female Anopheles mosquitoes desire to foster their eggs, they bite and draw blood from the human body. If a parasite infects that person, then that same infected parasite blood is found in the mosquito and that parasite reproduces and develops in the mosquito body. When that infected mosquito bites another person, parasites containing the salivary gland are transferred into that person's blood [5]. After transferring parasites into the human body by the mosquito, malaria parasites grow with very high speed in the liver and RBC of that infected person. Symptoms of malaria appear after one or two weeks. Primary symptoms that appear are headache, vomiting, fever, and chills. If malaria is not treated early and properly, it is very harmful to the human body. It may be a reason for kidney failure, low blood sugar, respiratory distress, enlargement of the spleen, etc. [6]. Malaria can kill a person by destroying their RBC. Malaria during pregnancy is very dangerous, and it is one of the reasons for abortion [7].
ere are five different protozoan parasite species, which are the main cause of malaria in the human body. ese are Plasmodium falciparum (P. falciparum), Plasmodium vivax (P. vivax), Plasmodium ovale (P. ovale), Plasmodium malaria (P. malaria), and Plasmodium knowlesi (P. knowlesi). Among all five species, the first four are the most common species, which occur in the human body. e fifth species is P. knowlesi mostly occurs in monkeys that live in South-East Asia forests. But, in past years, some cases of P. knowlesi malaria occurred in the human body. e most common species found in the human body is P. vivax, but the most dangerous species is P. falciparum [8]. Figure 1 shows the images of the different types of malaria found in human peripheral blood smears.
All species of protozoan parasites are morphologically different. At every stage of its lifecycle, each species changes in its size, color, shape, and morphology. ese various stages of every species are ring, trophozoite, schizont, and gametocyte, as shown in Figure 2. e main reason for the high mortality rate is the late detection of malaria. In medical science, for the detection of malaria, microscopic examination is the gold standard. A microbiologist manually counts affected RBC under the microscope to examine the patient's blood sample, which is a very time-consuming and highly tedious process. e accuracy of this process is entirely dependent on microbiologist expertise [10]. Hence, microscopic examination is a prolonged process, and it is the main reason for the late detection of malaria in patients, increasing the high mortality rate. e high malaria mortality rate can be decreased by detecting malaria at an early stage. erefore, an automated computer-assisted technique is needed, which will help the microbiologists to provide a second opinion for effective and early detection of malaria and reduce the mortality rate. e pattern of total worldwide malaria patients is illustrated in Figure 3. It represents how malaria patients are increasing worldwide. In 2013, 198 million malaria-affected patients were detected, which was increased to 229 million in 2019 [1]. ese very troubling statistics can be reduced by detecting parasites and diagnosis in the early stages, and it would be beneficial when experts are not available. e paper's contributions are as follows: (i) a comprehensive review has been conducted on the state-of-the-art techniques for malaria diagnosis that have been published in the last decade; (ii) various types of automated computational methods such as preprocessing, segmentation, feature extraction, and classification for diagnosing malaria have been discussed in detail; (iii) additionally, different types of machine learning and deep learning models, as well as their accuracies for malaria parasite detection and diagnosis, have been discussed; (iv) moreover, several types of blood smear image datasets for malaria diagnosis have been identified; and (v) various challenges and issues with the already implemented techniques and scope of future work have also been discussed. e paper is organized as follows: (i) Section 2 summarizes the state-of-the-art techniques for malaria diagnosis; (ii) Section 3 explains automated computational methods for diagnosing malaria in detail; (iii) Section 4 presents the discussion with research gaps; and (iv) Section 5 concludes the paper with future scope.

State-of-the-Art Techniques for Malaria Diagnosis
Malaria is a disease in which symptoms appear after 7 to 15 days. Primary symptoms are headache, vomiting, fever, pain, chills, etc. ese symptoms could be an indication of malaria, although many diseases have the same symptoms. Hence, some techniques are needed that can diagnose malaria correctly. For malaria diagnosis, different techniques have been developed such as microscopy blood smear examination, cytometry, rapid diagnostic test (RDT), polymerase chain reaction, and fluorescent microscopy. Still, for diagnosing malaria, the primarily used techniques are (a) microscopic thick and thin blood smears examination and (b) rapid diagnosis test in medical science [11].

Microscopic ick and in Blood Smears Examination.
In this, a laboratory examination is performed in which a clinician divides the blood sample into two parts on the slide. One is called a thick blood smear, and another is a thin blood smear. After that, a clinician manually counts the affected RBC under the microscope. A thick blood smear helps clinicians detect the presence of malaria parasites, and a thin blood smear helps identify the species of the parasites causing malaria. All the steps for malaria detection using microscopic blood smears examination are shown in Figure 4. Advantages of the microscopic technique are as follows: (i) a clinician can distinguish the different stages of malaria species at a very low cost using microscopic method and (ii) microscopy technique for malaria detection is more effective as compared to rapid diagnostic tests as it can count affected RBC very efficiently. Apart from the advantages of microscopic techniques for malaria detection, some challenges are also there. Microscopic thick and thin blood smears examinations technique accuracy depends on microbiologist experience. To detect and diagnose malaria through a microscope, a microbiologist may have to count malariaaffected RBC manually, which is a highly tedious and timeconsuming task [10]. It is found in multiple studies that manual counting of affected cells using a microscope is not an authentic technique when it is done by a nonexperienced microbiologist [13]. Instead of this, to confirm a blood smear slide is malaria-affected or not, a microbiologist needs 2 Computational Intelligence and Neuroscience significant time. But, it is a tough task for a microbiologist to examine each slide because a microbiologist has to study multiple blood smear images under the microscope. Moreover, this technique takes time to examine blood smear slides.

Rapid Diagnosis Test (RDT).
Rapid diagnosis test or antigen test is a small kit used to detect antigens derived from malaria parasites. To identify malaria, a drop of blood is inserted into the kit from the given hole, and internally, this Figure 1: Different types of malaria peripheral blood smear images (a) P. falciparum (b) P. vivax (c) P. ovale (d) P. malaria [9].   Computational Intelligence and Neuroscience device performs the tests and provides the result in minimum time. RDT kit functioning is shown in Figure 5.

Species
Advantages of the RDT kit are as follows: (i) it is significantly faster than manual cell counting techniques, and it gives instant results; (ii) for the use of the RDT kit, no expertise is required; and (iii) it is beneficial in endemic regions. Instead of the advantages of the RDT kit for malaria detection, some challenges are also there. As per the analysis of different studies, the results of this technique are less accurate, and any wrong result can affect the patient's treatment [14]. Another main challenge of the RDT kit is detecting whether a patient is malaria-affected or not. It cannot detect malaria species.
Hence, after studying different techniques of malaria diagnoses and their advantages and challenges, researchers observed that a computer-assisted malaria detection technique would be required. A computer-assisted malaria detection technique increases the performance of existing techniques by avoiding its limitations in terms of accuracy, instant results, dependency, and requirement of the expert microbiologist.

Automated Computational Methods for Diagnosis of Malaria
In medical science, the computer plays a very crucial role. Different automated computational methods are used for the diagnosis of multiple diseases. Ultrasound images, magnetic resonance imaging, X-ray images, and computed tomography images are used to diagnose different diseases of human anatomy using computerized imaging techniques. e computer-assisted diagnosis technique for malaria is based on the microscopic technique, which is performed by computer with the help of machine learning algorithms and computer vision techniques. is is the technique in which digital thin and thick blood smear images are used for the detection of malaria parasites automatically. Different steps of automated diagnosis of malaria are image acquisition, preprocessing, red blood cell detection and segmentation, feature extraction, and selection and classification (parasite identification and labeling). e stepwise process of automated computational methods for malaria parasite diagnosis is shown in Figure 6. In this section, a deep survey has been performed on each technique used for automated detection of malaria using blood smear images.
3.1. Acquisition of Image Dataset. Digital images of blood smear samples are required to detect malaria in a patient using computer vision image processing and machine learning techniques. Each patient's blood smear sample is distributed into two parts: thick and thin blood smear images. Most computer-assisted detection studies use thin blood smear digital images, and very few researchers have worked on thick digital blood smear images [16]. Figure 7 shows the images of thick and thin blood smears. A thick blood smear is a drop of blood that assists in detecting the presence of parasites, and a thin blood smear is a layer of blood that is spread on a glass slide and assists in identifying the species of the parasite causing the infection. Different sources collect digital blood smear images, and this process is called the image acquisition technique. Categorization of different image acquisition techniques used on blood smear images for malaria parasite detection is shown in Table 1.
After analyzing the different image acquisition techniques in Table 1, we observed that there are various image acquisition techniques available. Still, the light microscopy technique is the most widely used and preferred because it has a high magnification factor, and it is beneficial for viewing the surface details of a blood smear.
Furthermore, Table 2 lists the different datasets of light microscopy techniques used by various researchers.

Preprocessing.
Preprocessing is a technique used to remove the unwanted noise and produce high contrast digital blood smear images for the next step. When different   resources take blood smear images, the images are corrupted by noise, and thus, visualization of the images is not good. Due to this problem, further steps of segmentation and classification are challenging to implement, and it produces poor results. Hence, certain preprocessing techniques have been used to remove that unwanted noise from images. Preprocessing techniques remove the noise from the image for better visualization, which is very useful for further analysis [46]. As shown in Table 3, researchers used multiple preprocessing techniques such as median filter, mean filter, low-pass filter, morphological filter, partial contrast stretching, local histogram equalization, Laplacian filter, SUSAN filter, geometric mean filter, Gaussian filters, and Wiener filter for enhancing the contrast and remove the unwanted noise of digital images. May et al. have given an approach in which the median filter technique for removing the impulse noise from digital images and for removing additive noise has been used [4]. e Gaussian filter is used by Arco et al. to enhance the quality of the images affected by Gaussian noise. e geometric mean filter is also used by Das et al. for preserving the edges and removing Gaussian noise from the digital microscopic image [66]. Laplacian filter is used by Savkare et al. for smoothening and enhancing edges of malaria parasite images [29]. To preserve the structure of an image, Susan's filter is suggested by [41]. To remove the intensity of high frequency from a digital image, a low pass filter has been suggested by [55]. e histogram matching technique is used by Abbas et al. to normalize the intensity value of digital image pixels [67]. e categorization of preprocessing techniques to enhance the quality of digital blood smear images is shown in Table 3. Table 4 displays the image preprocessing approaches used by various researchers for better visualization of thin and thick blood smear images with their properties.

Red Blood Cell Detection and Segmentation.
Segmentation is the process in which digital images are disjoint into nonoverlapping regions. Each disjoint image typically corresponds to other parts of an image object. Once each digital image object is isolated, each object can be easily measured and classified. In the literature, different segmentation techniques have been applied on digital blood smear images to detect ROI (region of interest).
Das et al. have developed an automated system for classifying malaria at different stages. e researcher used the watershed segmentation technique in their research work for the segmentation of thin blood smear digital images.
is technique provided better results for detecting erythrocytes from the whole blood smear image [66]. Further, a watershed algorithm is suggested by Savkare et al.  -- [29] 68 images Light microscopic images of different magnification have been used. [25] 300 images Used KNN classifier and got 90.17% accuracy to detect malaria parasite species. [26] 27578 images 27578 single cell light microscopy images were used, and a new 16-layer CNN model was proposed to identify malaria-infected or infected images. [23] 160 images Achieve 95% accuracy for the detection of malaria.
[45] -Used Giemsa-stained blood smear images were taken by a camera attached with a microscope on 1000x magnification, and the proposed model got 77.78% accuracy. [19] 27558 images Implement novel stacked convolutional neural network technique for parasite detection.
-Not reported by the original paper. is technique segmented the RBC and parasites from the blood smear image based on hue range and optimal saturation thresholds [69]. Mandal et al. introduced a normalized cut method for microscopic blood smear images segmentation.
e segmentation algorithm has been used on different color spaces to find the optimal performance of digital blood smear images [70]. e result of the normalized cut segmentation algorithm is good in HSV color space. Nasir et al. have presented a segmentation-based approach using a K-means clustering algorithm for the segmentation of malaria parasite on 100 digital blood smear images dataset [71]. Bhatia also proposed a K-means clustering technique using genetic methods [72]. Panchbhai et al. have reported the RGB color space model and Otsu algorithm for RBC and parasite segmentation from 20 thin blood smear images [73]. For microscopic blood cells, digital images segmentation, the K-means clustering technique, and global threshold techniques are suggested by Savkare and Narote [74]. In this, 78 microscopic blood cell digital images are used for segmentation. Khan et al. also used the K-means clustering for the segmentation of 118 blood smear images to identify malaria parasite tissues [75]. Acharya et al. introduced a new computer-assisted detection technique for segmenting blood smear images and determining the acute myeloid leukemia stage (AML). is work's approach is divided into many stages. A unique algorithm is being developed to accurately segment blood smear images in order to identify AML and its variants. e classification accuracy of the model was 99.48% on 500 test images [76].
To detect the exact radius of RBC, the circle hough transformation method was introduced by Ma et al. [77]. Otsu thresholding clustering-based method is presented by Makkapati et al. to get the image mask of binary image [78].
Deep learning techniques are also beneficial in image segmentation. Researchers for image segmentation have proposed many deep learning techniques. For image segmentation, a fully convolutional neural network-based deep learning technique has been proposed by Long et al. [79] and Wang et al. [80]. A completely CNN encoder and decoder deep learning segmentation technique (SegNet) has been used by Badriinarayanan et al. [81]. Ronneberger  is approach is primarily based on the image classification model ResNet [84].
Segmentation techniques on blood smear images used in different studies are summarized in Table 5. After analyzing

Feature Extraction and Selection.
Feature extraction after segmentation is a prerequirement for feature selection and classification. e objective of feature extraction is to recognize and characterize an object whose dimensions are very nearest or similar for objects in the same class and different for objects from a different class. It reduces the  To extract the features of haralick textures, mean, entropy, roughness, homogeneity, and standard deviation, Das et al. suggested gray-level co-occurrence matrix [66]. To extract the intensity-based features, Chayadevi and Raju used a color channel intensity algorithm [96]. Rajaraman et al. have given a pretrained model for the feature extraction and the detection of malaria parasites [101]. In this, a pretrained convolutional neural network including AlexNet, VGG-16, Xception, ResNet-50, and DenseNet-121 are used for extracting features from infected and uninfected 27558 cell images. e developed model for feature extraction and malaria parasite detection took more than 24 hours for training and produced 95.9% accuracy for malaria parasite detection in thin blood smear images. To identify the texture features from a blood smear image to detect malaria parasites, Chavan and Sutkar used a histogram-based feature extraction method [102]. e color histogram feature extraction technique is used by [43] for identifying infected erythrocytes from blood smear images. Reference [103] extracted features of RBC size and shape, RBC texture, and parasite shape from the thin blood smear images, and used these features to classify malaria parasite species. For extracting the features from digital microscopic images based on morphological, [43] used a granulometry algorithm.
Various features of extraction and selection techniques implemented by various researchers for malaria blood smear images are shown in Table 6. As evident from Table 6, it is found that researchers used different feature extraction techniques based on their goals. Mostly used feature extraction techniques were color features and texture features. However, some authors recommended morphological feature technique for features extraction from malaria blood smear images [51,108].

Parasite Identification and Labelling (Classification).
Classification is a technique to identify a pattern that belongs to which class. In this literature, different authors developed different classification techniques to identify a patient, whether he or she is malaria-affected or not. So, there are two classes to detect whether the patient is affected by malaria or not.
Vijayalakshmi and Kanna have introduced a deep learning approach to classify infected and noninfected falciparum malaria. e presented technique was achieved by the visual geometry group (VGG) network and SVM. In this, 1530 malaria digital corpus images have been used for training and testing the model. In this, the transfer learning approach to train the model is used in which we trained the top layer of the model and freeze the rest out of the layers approach applied. For the classification of infected or noninfected falciparum malaria, the given model obtained 93.13% accuracy [8].
For the classification of malaria-infected stages from thin blood smear images, Das et al. used five different classifiers to classify the malaria-infected stages. ese five classifiers are Naive Bayes, Logistic regression, Radial Basis Functions (RBF) neural network, Multilayer perceptron neural Computational Intelligence and Neuroscience ✓ ✓ RGB-red green blue; HSV-hue, saturation, and value; CCM-color co-occurrence matrix; GLRLM-Gray-level run length matrices; GLCM-gray-level co-occurrence matrix; LBP-local binary pattern; WT-wavelet transform; GT-gradient texture.
network, and classification and regression tree. In this, 888 erythrocytes infected and noninfected image dataset is used. Out of this, 592 labeled images are used to train the classifiers and the remaining are used for testing the classifiers. e experimental results show that among all five classifiers, the multilayer perceptron network has provided better results than the other four classifiers on the 750 images dataset [66]. Seman et al. developed a multilayer perceptron network (MLP) to classify different malaria parasite species from thin blood smear images.
is work classified three different species from malaria parasites: P. falciparum, P. malariae, and P. vivax [103]. e authors used the backpropagation algorithm of the MLP network for training and compared the results of the MLP network with Levenberg-Marquardt and Bayesian rule algorithms. MLP network has produced better results as compared to the other two algorithms.
Otsu thresholding method is used by Malihi et al. for the classification of four species of malaria parasites in blood smear images. is technique has provided better results in comparison with other techniques. In this, 363 blood smear images are used and obtained 91% accuracy [43].
Further, Anggraini et al. have classified the different stages of malaria parasites using a Bayesian classifier on 110 thin blood smear images and obtained 93.3% accuracy [112]. Minimum distance classifier technique has been given by Ghate et al. for detecting the presence of malaria parasites using 80 blood smear images and got 83.75% accuracy [39]. Savkare and Narote presented Otsu thresholding, watershed transform, and SVM binary classifier to classify normal and parasite-infected cells [113]. Das et al. have presented the Bayesian approach for automated screening of malaria parasite from microscopic images [51].
Kareem et al. have developed an automated technique for detecting malaria parasites in thin blood smear images. In this, a dataset of more than 200 images is used. Two methods of classification for parasites are used. e first one is based on relative size and morphology, and the second is based on intensity variation. e final results of the developed model have shown an accuracy rate of 87% [36].
Prasad et al. have presented a decision support system approach to classify the infected and noninfected malaria parasites in thin blood smear images. In this, 200 thin blood smear images have been used, and 96% accuracy has been obtained [114]. Rosado et al. have developed a supervised classification technique to detect malaria parasites in blood smears. In this, machine learning (ML) classification 10-fold cross-validation for WBC (white blood cell) and P. falciparum trophozoites detection has been performed and got 91.8% accuracy [85].
Mohammed and Abdelrahman have given a technique for detecting and classifying malaria from 160 thin blood smear images taken from the Centre for Disease Control and Prevention (CDC). To extract the RBC from blood smear images, researchers used morphological processing. is technique found the parasites and overlapped cells in the image. Based on the number of RBCs in each image, RBC is classified into two classes: infected and noninfected cells. After that, a normalized cross-correlation algorithm was employed to classify the affected blood smear parasite into four different malaria species. e given method has produced 95% accuracy for detection [23]. Saiprasath et al. evaluated seven different machine learning algorithms on the same malaria image dataset and concluded that Random Forest outperforms every other algorithm, closely followed by the Ada Boost algorithm [115].
Bibin et al. have given an automated technique to detect malaria parasites in peripheral blood smear images. e given binary classifier is based on deep learning, which used 1978 images to train and test the technique and achieved 96.21% accuracy [106]. Simon et al. suggested a CNN-RNN model for malaria detection. Compared with the CNN-LSTM and CNN-GRU models, the proposed model generated the best results [116]. Dev et al. suggested a hierarchical convolutional network and produced better results than prior studies [117].
Dave et al. used adaptive thresholding, erosion, and dilation operations to diagnose malaria from 117 blood smear microscopic digital images and got 89.88% accuracy [34]. Oliveira et al. have suggested the face detection algorithm to identify Plasmodium parasites from blood samples. In this, a dataset of 1332 blood sample images has been taken and shown 91% accuracy [118].
Mohanty et al. have presented the autoencoder (AE) neural network unsupervised technique to identify malaria in blood smear images. In this, the AE technique has been compared with the SOM technique. e AE technique obtained 87.5% accuracy compared to the 79% accuracy of the SOM technique. 1182 blood smear images have been used to perform experiments [119]. Morales-Lopez et al. suggested the SVM technique for classification problems [120]. Table 7 has listed different types of classification techniques used for the identification of malaria parasites.
After the analysis of Table 7 and literature of malaria parasite classification techniques, it is found that various classification techniques that researchers commonly implement are CNN, SVM, and TL-VGG classifiers.

Discussion
In the last decade, a lot of experiments have been done in the area of automated detection of malaria to reach the current state-of-the-art. In this study, different computational methods implemented on various stages of computerassisted techniques for detecting malaria parasites using blood smear images have been examined. Image acquisition is the first and very important step for automatic detection of the malaria parasite. e present study shows various techniques for acquiring digital blood smear images, but the light microscopy technique is the most widely used and liked technique by researchers. ere is a number of computational methods out of which preprocessing is the first step in image analysis.
Preprocessing is one of the crucial stages implemented on acquired digital blood smear images. It plays a crucial role in detecting infected RBC by removing the unwanted noise and producing high contrast digital blood smear images without demolishing the image features. As per the current study, median/mean filter, morphological filter, Laplacian Computational Intelligence and Neuroscience Achieve better results by increasing dataset size and using 2 or more classifiers. [85] Used SVM classifier for WBC and P. falciparum trophozoites detection. 1843 malaria images 91.8 Implemented only with the mobile-based framework. [125] Used image processing and artificial intelligence techniques and face detection algorithm to identify plasmodium parasites from blood samples.

malaria images 91
Detected only 1 malaria parasite, and more algorithms can explore to achieve better accuracy. filter, Susan filter, and Gaussian low-pass filter are mostly used techniques by researchers to remove unwanted noise and increase the contrast of the images. Segmentation is the next stage after the preprocessing, which is used to segment the RBC to detect malaria parasites using blood smear images to facilitate the classification process. As per the study of literature, mostly used segmentation techniques by researchers are as follows: (i) Otsu thresholding for segmentation of parasite RBC; (ii) Marker-controlled watershed and Edge detection algorithm is used at the segmentation phase; and (iii) for the segmentation of overlapping cells, the watershed algorithm has been widely used. After segmentation, blood smear images have been classified to diagnose malaria-infected or not infected by feature extraction and selection techniques. As per the study, Color features, Texture features, and Morphological features have been mostly used feature extraction techniques for early diagnosis of malaria from blood smear images. From the literature, it has been observed that the maximum accuracy of 95.03% has been achieved by CNN based deep learning model in comparison with the VGG-SVM model [8,101,126].
A thorough review of the literature on automated analysis of malaria parasite using blood smear images yielded the following challenges and future directions: e accuracy of an automatic image classification model depends upon multiple aspects such as analysis of the digital blood smear image depend on the staining method, magnification factor of an image, condition of nearest environment where the digital image has been collected like the background of the image, light in the room, and most important quality and position of the camera. erefore, a standard digital blood smear dataset is necessary to test and validate the model to obtain efficient and reliable results.
Many researchers have performed their experiments and published their articles in the same area. Moreover, an automated computational-based computer vision method, which should be efficient and effective for automated detection of the malaria parasite from blood smear images, needs to be improvised according to the requirement of the community. e community requires (i) standard image dataset because researchers' datasets are mostly unstandardized.
e digital blood smear dataset depends on the characteristics and quality of the microscope as all digital images of blood smears are taken by a digital camera attached to a microscope. So, a well-standardized dataset is most important for a machine learning algorithm for automated detection of malaria. (ii) In the literature, developed methods can recognize only one type of malaria parasite [8]. But, the patient may be affected by more than one parasites species. Hence, there is a need for such a model that can recognize different types of malaria parasites. (iii) To classify malaria parasites from blood smear images, authors trained the machines with different models and techniques. e training model is taking a long time to learn [66]. Hence, there is a necessity to reduce the training time to train the classification models. (iv) In literature, developed models by different researchers analyze the blood smear digital images that are taken from a camera that is attached with a microscope [4,8]. Hence, there is a demand for a model to analyze thin blood smears images acquired exclusively with smart phones [43]. (v) A technique that different authors use to diagnose malaria is invasive, in which an injection syringe takes a blood sample. erefore, there is a requirement for a noninvasive technique that can be used to detect malaria [128]. (vi) After the analysis of Table 7, it has been found that all the existing state-of-the-art techniques used to detect malaria from microscopic blood smear images are not very accurate. Each technique has some limitations and challenges. erefore, there is a necessity for an automatic technique that can improve the accuracy for the detection of malaria parasites and it will also help in early detection of malaria and reduce the mortality rate in future.

Conclusion
is study is a solid starting point for researchers who want to look into automated blood smear analysis to detect malaria.
is study reviews and discusses computer vision and image analysis works that target the automated detection of malaria on blood smear images. In this paper, we have discussed the present facts of necessary components of computer-assisted technique: (i) acquisition of image dataset, (ii) preprocessing, (iii) segmentation of RBC, (iv) feature extraction and selection, and (v) classification, which have been used to diagnose malaria parasite from blood smear images suggested by various researchers. Digital blood smear images taken from a microscope may affect how and which malaria parasites are detected. After analyzing segmentation and classification state of the art techniques, it has been observed that future computer-assisted techniques should be based on standard datasets and magnification factors to detect malaria parasites. e complexity of different classifiers of machine learning that are based on deep learning increases as the number of layers increases. To achieve efficient and reliable results, a large dataset is required for training and testing. With the help of computational methods such as data augmentation and deep learning methods, the computer-assisted method can obtain better results.

Reference
Technique used Dataset Accuracy (%) Limitations/challenges [127] Used custom CNN that consists of three fully connected convolutional layers.

malaria images
However, some state-of-the-art techniques are presented in the literature, but still, there is a huge scope of future work, which may help the microbiologists in the detection and diagnosis of the malaria parasite at an earlier stage to reduce the mortality rate such as (i) different computational methods that are used to collect blood smear images physically can be studied more to enhance the segmentation results to detect infected RBC very effectively. Hence, an efficient computational method of infected RBC segmentation can be developed. (ii) Various feature extraction methods such as color features, texture features, and morphological features [51,106,108] can be analyzed more, which will be very helpful for the development of an efficient automated computer-assisted system to detect infected malaria RBC using blood smear images. (iii) To classify malaria blood smear images, mostly implemented techniques are SVM, K-means, and VGG classifiers. Still, there is a vast scope to implement customize CNN algorithms to detect infected malaria RBC with high accuracy. If the CNN model is implemented on blood smear images at a minimum magnification factor for classification, it may decrease the cost and time complexity of the system.
In the field of malaria detection from blood smear images, the contribution of many research publications is noteworthy. However, this study has tried to present opinions to the microbiologists and technical community. It will be very helpful for them to generate an effective and efficient computer-assisted technique for malaria detection at an early stage. [129,130].

Conflicts of Interest
e authors stated that there are no conflicts of interest.