Clinical application of a single-operator direct visualization system improves the diagnostic and therapeutic yield of endoscopic retrograde cholangiopancreatography

Division of Gastroenterology, University of Alberta Hospital, Edmonton, Alberta Correspondence: Dr Gurpal Sandha, Division of Gastroenterology, University of Alberta Hospital, Zeidler Ledcor Centre, 130 University Campus, Edmonton, Alberta T6G 2X8. Telephone 780-492-8170, fax 780-492-1699, e-mail gurpal.sandha@ualberta.ca Received for publication May 23, 2012. Accepted June 4, 2012 The assessment of biliary strictures remains a significant challenge. Malignant strictures (cholangiocarcinoma) often grow longitudinally rather than in a radial fashion and, thus, large tumour masses are often absent on modalities such as abdominal ultrasound, computed tomography and magnetic resonance imaging (1). Inflammatory strictures (eg, primary sclerosing cholangitis [PSC]), especially with their potential for developing malignancy, are also equally difficult to accurately diagnose and follow. Current clinical practices, such as biliary brush cytology and endoscopic retrograde cholangiopancreatography (ERCP)-directed biopsy acquisition, have a less than optimal sensitivity of 33% to 58% (2-4). False-negative results can delay life-saving interventions (5). On the other hand, false positives can result in unnecessary surgical intervention, thereby increasing morbidity and health care costs. The need for improved efforts to obtain accurate and directed tissue diagnosis cannot be overemphasized. To enable direct visualization and have the ability to perform biopsies of lesions within the bile duct, flexible cholangioscopes were developed in the 1990s and have been used in a procedure often referred to as ‘mother-baby’ cholangioscopy (6). The procedure requires two experienced endoscopists, one to work the ‘mother’ duodenoscope and the other to operate the ‘baby’ cholangioscope. The latter is introduced through the working channel of the ‘mother’ scope. This has proven to be a cumbersome procedure and has not achieved widespread use. In addition, the ‘baby’ cholangioscope only has two-way tip deflection capability, limiting its manoeuvreability, and is subject to increased repair costs because of its fragility. The ‘SpyGlass’ (Boston Scientific, USA) cholangioscopy system is a relatively recent advance that enables one endoscopist to manoeuvre both the duodenoscope and the cholangioscope, enabling direct visualization of the bile duct. Studies have recently documented its clinical usefulness not only for the diagnosis of strictures (with biopsy) but also for therapeutic (with electrohydraulic lithotripsy [EHL] or laser lithotripsy) intervention of biliary stones refractory to conventional treatment (7-11). The objective of the present study was to describe our experience with SpyGlass cholangioscopy in managing biliary disease. original artiCle

T AlAmeel, V Bain, G Sandha.Clinical application of a single-operator direct visualization system improves the diagnostic and therapeutic yield of endoscopic retrograde cholangiopancreatography.Can J Gastroenterol 2013;27(1): 15-19.

MeThodS
The present analysis was an observational study evaluating data from all patients who underwent diagnostic and/or therapeutic SpyGlass cholangioscopy at the University of Alberta Hospital in Edmonton, Alberta.All procedures were performed by two experienced biliary endoscopists (VB and GS), with individual ERCP experience of >12 years, having performed >2000 procedures each.The SpyGlass cholangioscopy system is a single-operator, direct visualization system that is introduced via the working channel of a standard duodenoscope (Olympus TJF180 therapeutic duodenoscope, Olympus America Inc, USA).The SpyGlass system consists of a disposable SpyScope access and delivery catheter, a 10 Fr catheter with four-way tip deflection capability, channels for accommodating optical and accessory devices and two channels for saline irrigation, the reusable SpyGlass direct visualization optical probe for visual examination of the pancreaticobiliary ducts and the disposable SpyBite biopsy forceps for tissue acquisition (7).The generator and probes for EHL and laser lithotripsy are additional accessories acquired separately (see below).

outcomes and procedure
The data were analyzed using an intent-to-diagnose approach for the assessment of biliary strictures and an intent-to-treat approach for biliary stones.Biliary strictures: Using the intent-to-diagnose approach for biliary strictures, the ability of SpyGlass to aid in making a visual diagnosis and then to biopsy any mucosal abnormality identified using the SpyBite forceps was assessed.The inability to make a visual diagnosis or to obtain biopsies, where indicated, was considered a failure of the procedure.The visual diagnosis was categorized as a malignant stricture (ie, cholangiocarcinoma) or a stricture resulting either from a benign, inflammatory process (ie, PSC) or from extrinsic compression (eg, carcinoma of the gallbladder, pancreatic adenocarcinoma or autoimmune pancreatitis).
The visual diagnosis with SpyBite tissue histology was individually correlated with surgical histology or clinical follow-up and SpyBite biopsy diagnosis with either surgical histology, where available, or clinical follow-up.The accuracy of both the visual ability of SpyGlass cholangioscopy to establish a diagnosis as well as the ability of SpyBite biopsies to confirm histology has been reported by previous investigators (7)(8)(9).
An attempt was made to make an overall SpyGlass diagnosis in all patients.This was the final impression of the endoscopist and was based on information obtained from both the visual and tissue diagnosis.This overall diagnosis was made based on the most concerning interpretation.For example, if a stricture was considered to be malignant by visual diagnosis and the biopsy was reported benign and/or inflammatory, a diagnosis of a malignant stricture was entertained as the overall SpyGlass diagnosis.
Because the present study was observational in nature and reports the institution's early experience with SpyGlass cholangioscopy, a follow-up time period was not defined a priori but simply reported at the time of preparing the manuscript.Figure 1 is an example of PSC in which only a visual diagnosis was made.The mass lesion shown in Figure 2 was found in a patient with PSC who underwent SpyBite biopsy.Figure 3 is an example of an apple-core lesion that appeared malignant on visual inspection and then underwent SpyBite biopsy.Biliary stones: For biliary stone lithotripsy, an EHL pulse generator (Northgate Technologies Inc, USA) was used.The intent-to-treat approach involved an attempt to visualize and fracture the targeted biliary stone(s) and eventually extract all fragments with the aim of bile duct clearance.The settings used on the pulse generator were as follows: power 30 W to 50 W, with a maximum of up to 100 W, if needed; and frequency 10 pulses/s to 30 pulses/s for 2 s.EHL is performed most effectively while irrigating with normal saline.After stone fragmentation, the need for additional intervention to aid in stone extraction (eg, balloon dilation of the sphincter and/or the use of balloon and/or basket extraction catheters) was left to the discretion of the endoscopist.If repeat ERCP was needed for EHL, this was considered in the analysis as a separate procedure, but if the repeat ERCP was simply to extract stones without the need for SpyGlass and/or EHL, these repeat procedures were not included in the final analysis.Inability to fracture the stone(s) with EHL was considered a failure of SpyGlass cholangioscopy.Figure 4 presents the various steps in the process of EHL with stone fracture and extraction of fragments from the bile duct.All patients who underwent SpyGlass cholangioscopy underwent at least one previous ERCP with sphincterotomy and/or stent placement, either by one of the two study endoscopists or by the referring endoscopist.The SpyGlass procedures were planned electively and not performed on an ad hoc basis, in part, because the infection control policy at the institution requires cleansing and disinfection of the reusable SpyGlass fibreoptic probe at least 24 h before the procedure.All cholangioscopy procedures were performed under general anesthesia because prolonged procedures were anticipated and operators preferred not to have suboptimal sedation become a potential explanation for inadequate procedure performance.

Figure 1) Common bile duct stricture in a patient with primary sclerosing cholangitis exhibiting smooth cicatrization without the presence of any mass lesion
There was no funding available for the present study and it was approved by the local institutional health research ethics board.

Statistical analysis
Descriptive statistics included mean, SD and range.Statistical analysis of the data with calculation of sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) was performed using two-way contingency table analysis.

reSulTS
In the first 12 months (starting April 2011), 30 patients (17 women) with a mean age of 66 years (range 41 to 89 years) underwent ERCP with SpyGlass cholangioscopy.The mean (± SD) procedure time for the entire cohort was 61±20 min (range 20 min to 119 min).Table 1 summarizes the patient demographics and procedure indications, along with outcomes of intervention and corroborating information where available.The data were analyzed on an intention-to-diagnose and/or intent-to-treat basis.
The two main indications for SpyGlass cholangioscopy were assessment of undiagnosed biliary strictures and EHL of biliary stones that were refractory to previous attempts with conventional modalities.The analysis of the results for these indications will be discussed separately.

Biliary stricture assessment
Of the 30 patients, 20 (67%) with a mean age of 64 years (range 41 to 82 years) were referred for assessment of biliary strictures.There were 12 women (60%).The location of stricture was in the distal one-third of the bile duct in five patients and in the proximal two-thirds in the remaining 15 patients.The mean procedure time was 55±16 min (range 20 min to 87 min).The mean follow-up period was five months (range two to 12 months).Only four patients had corroborating surgical histology available.Three other patients died within the follow-up period, presumably of malignant disease, although confirmation by autopsy was not available.

SpyGlass visual diagnosis:
The overall success of SpyGlass cholangioscopy in identifying the biliary stricture and making a visual diagnosis with respect to location, length and surface characteristics was 95% (19 of 20 patients).A distal stricture could not be visualized in one patient.The sensitivity (83%), specificity (84%), PPV (71%), NPV (91%) and overall accuracy (84%) of the ability of SpyGlass to make a visual diagnosis are shown in Table 2.

SpyBite tissue diagnosis:
Of the 20 patients, SpyBite biopsies were attempted and obtained in 16 patients.Biopsies were not performed in four patients in whom no obvious mucosal abnormality was apparent.

Biliary stone management
Ten patients were referred for EHL for difficult stones.These patients were generally older (mean age 72 years, range 52 to 89 years) and sex was more evenly distributed (five females [50%]) than the biliary stricture cohort.Furthermore, the mean procedure time was longer for this group (72±25 min, range 36 min to 119 min).The indications for EHL in these patients were impacted stones, multiple large and faceted stones, and stones situated above a biliary stricture.
The overall success with an intent-to-treat approach was 90% (nine of 10 patients).EHL was attempted and successful in eight of nine patients (89%), and eventual clearance of the duct was achieved in all patients.No patient required more than one SpyGlass or EHL session, although one patient with multiple large stones underwent a repeat ERCP for extraction of stone fragments and duct clearance.In one patient referred for a reportedly 'large' stone, a stone could not be visualized despite cholangiography and cholangioscopy.No subsequent intervention has been performed in this patient in 11 months of follow-up.One patient had a 1 cm stone situated distally in a dilated bile duct.Adequate visualization was never achieved and, because EHL could not be performed, this was considered to be a failure in the intent-to-treat analysis.The stone was removed during the same procedure after balloon dilation of the sphincter and may not have initially been a good indication for SpyGlass.

Complications
There has been no major complication associated with SpyGlass cholangioscopy in our experience.One patient developed post-ERCP pancreatitis and recovered well with conservative treatment.One patient with cholangiocarcinoma developed an altered level of consciousness and acute urinary retention 24 h after the procedure and died three days later.An autopsy was not performed and it remains unclear whether the events were related to the SpyGlass procedure.

diSCuSSion
Evaluation of undetermined biliary strictures remains one of the most challenging tasks in pancreaticobiliary endoscopy.Conventional methods of investigation, including cross-sectional abdominal imaging, are poor at accurately defining benign and/or malignant lesions of the bile duct.Attempts at obtaining tissue for diagnosis by ERCP with brush cytology and/or ERCP-directed forceps biopsies within the bile duct have not yielded consistent and widely applicable results (2)(3)(4).Even the initial promise of endoscopic ultrasound-guided fine-needle aspiration has not translated into widespread use despite favourable results with respect to sensitivity and specificity (12)(13)(14).
Direct intraductal visualization of biliary pathology has long been recognized as the best way to evaluate strictures.'Mother-baby' (also known as 'mother-daughter') biliary endoscopy has been in clinical use for many years (6).However, this procedure also has not seen widespread acceptance, in part, because of the need for two endoscopists.To overcome this particular disadvantage, the SpyGlass single-operator direct visualization system was devised and first clinically tested in 2007 by Chen and Pleskow (7).Since then, various groups have reported their initial experience and success, although only a handful of these studies have collected data in a prospective manner (8,10).
In these initial years of investigation with SpyGlass, several outcome measures have been evaluated.One of these is procedural success, which has been reported by most investigators to be significantly greater than 90% (7)(8)(9)(10)(11).The criteria to diagnose strictures have focused not only on the ability of SpyGlass cholangioscopy to identify the stricture and make a visual diagnosis (sensitivity and specificity ranging between 71% and 95%, and 79% and 100%, respectively) but also on the ability of SpyBite forceps biopsies to confirm the initial visual diagnosis (sensitivity and specificity ranging between 49% and 82%, and 82% and 98%, respectively) (7)(8)(9)(10)(11).Our results are also   consistent with these published data.Furthermore, the accuracy of SpyGlass in being able to make an overall diagnosis is 85%.This is certainly a significant improvement from the existing standard of care, which includes bile duct brushings and, less often, attempts with ERCP-guided biopsy.One of the limitations with analyses relating to biliary strictures with SpyGlass cholangioscopy is the consistent lack of a corroborating 'gold standard' for verification of the diagnosis.It is easy to compare SpyGlass intervention (visual and/or biopsy results) against surgical histology, where available; however, as is more common, a vast majority of these patients are deemed inoperable candidates.This leaves investigators to assume the presence of malignancy based on patient survival rather than actual histology.The sensitivity of SpyBite tissue diagnosis in our study (40%) was less than optimal, and may be explained by the relatively low number of cases and also by the fact that these data are the result of our initial experience with SpyGlass cholangioscopy.We believe that this is likely to improve with increasing experience.Our initial results are comparable with the early experience of other investigators who have had a similarly low sensitivity of SpyBite tissue diagnosis as reported in a recent multicentre registry (9).Moreover, in benign strictures of the bile duct, such as PSC, similar corroborating histology is usually unavailable.Prolonged survival, usually >6 months, is assumed to indicate benign disease.This lack of a comparative 'gold standard' is one of the limitations of our study, as it has been for previous investigators.
The published results of intention-to-treat analyses of stone fragmentation and extraction using SpyGlass cholangioscopy are even better, with reported success rates of 92% to 100% (9,10).This is significant given that these particular stones had previously failed conventional attempts at extraction and could have undergone more invasive interventions, including open bile duct exploration, for extraction.Our 90% success rate with EHL for stone fragmentation and subsequent extraction is also consistent with data reported in the literature.
SpyGlass cholangioscopy has certainly proven to be effective in the investigation of biliary strictures and treatment of biliary stones.However, there are certain technical limitations that warrant mention.This is a fibreoptic technology and image quality has been one of the major drawbacks, especially when compared with imaging using 'mother-baby' video cholangioscopes.However, the development of high-definition video technology in the near future is inevitable and will certainly enhance image quality.Nonetheless, given the current constraints with this technology, and that these procedures are time consuming, visualization can be optimized by adequate irrigation and proper drainage and suctioning through a generous sphincterotomy.This, however, can sometimes be difficult proximal to a stricture.In our experience, free irrigation can be performed provided suctioning is performed by the assisting nurse with a syringe attached to the operating channel of the SpyScope access catheter.This can eliminate debris and improve image quality.
We also believe that the distal bile duct is the most technically challenging area to visualize.This likely occurs because the tip of the SpyScope access catheter and, hence, the SpyGlass probe, abuts the wall of the bile duct as it straightens coming through the distal bile duct.Some investigators recommend examining the distal bile duct in a 'long' ERCP position so as to align the access catheter with the long axis of the bile duct and position it in the middle of the lumen.
Our study was not a prospective controlled analysis but rather an observational study in which we assessed data from each consecutive patient who underwent SpyGlass cholangioscopy.This design is bound to introduce potential bias in making a visual diagnosis if the clinical history and investigations are known to the endoscopist.This bias could be excluded if the endoscopist performing the procedure is blinded to clinical information.However, because this was an observational study involving a new technical procedure, we believed that the endoscopist should have as much clinical information available as possible to maximize the diagnostic and therapeuitc yield of SpyGlass cholangioscopy.

ConCluSion
SpyGlass cholangioscopy is a significant advance in the direct evaluation of the biliary tree and allows for a single endoscopist to directly visualize and biopsy strictures for tissue diagnosis, and perform EHL fragmentation of stones that are otherwise refractory to conventional modalities of extraction such as balloon/basket extraction, mechanical lithotripsy and dilation-assisted balloon extraction.It is a time-consuming -albeit safe -procedure and efficacy is bound to improve with increases in operator experience and improvements in technology, especially those targeting optical optimization.diSCloSureS: Dr Gurpal Sandha is a consultant for Boston Scientific.
The other authors have no finacial disclosures or conflict of interest relevant to this article.This study was presented, in part, as an abstract at Canadian Digestive Diseases Week, February 23 to 27, 2012, Montreal, Quebec.

Figure 2 )
Figure 2) A series of images from a patient with primary sclerosing cholangitis with a mass lesion.A Normal distal bile duct.B The beginning of a mural-based mass.C and d The mass is clearly visible and was biopsied with SpyBite forceps (Boston Scientific, USA)

Figure 4 )
Figure 4) These images reveal the steps during electrohydraulic lithotripsy (EHL) of a large biliary stone.A Cholangioscopic view of the stone with EHL probe visible.B Initial fracture site in the stone.C Fragmentation of the stone with sequential EHL applications.d Extraction of fragments with balloon-extraction catheter

TAblE 1 baseline patient demographics with clinical characteristics and procedure outcome
§ Indication, n Multiple/large/faceted stone(s) 3 Impacted stone(s) 3 Stone(s) above stricture 2 Electrohydraulic lithotripsy 8 Stone extraction, n (>1 may apply)*Boston Scientific, USA; † Visualization could not be achieved in one patient; ‡ No lesion identified in four patients; therefore, no biopsy attempted; § One patient had no stone identified and, in another patient, there was difficulty visualizing the stone.Electrohydraulic lithotripsy was not performed in these two patients