Coming full circle : From antibiotics to probiotics and prebiotics

1Departments of Pathology and Laboratory Medicine, Medicine, and Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta; 2Queen Elizabeth II Health Sciences Centre and Dalhousie University, Halifax, Nova Scotia Correspondence: Dr John Conly, Departments of Pathology and Laboratory Medicine, Medicine, and Microbiology and Infectious Diseases, Room 930, 9th Floor, North Tower, 1403 29th Street Northwest, Calgary, Alberta T2N 2T9. Telephone 403-944-8222, fax 403-944-1095, e-mail jconly@ucalgary.ca Received and accepted for publication May 25, 2004 Antibiotics are used as agents to prevent and treat infections caused by pathogenic bacteria and other microbes, and rank as one of the most important developments of modern medicine. The word ‘antibiotic’ is derived from the Greek term ‘biotikos’ and may be literally translated as ‘against life’. Antibiotics were first used centuries ago by the Chinese in the form mouldy soybean curd applied to carbuncles and furuncles. (1) The ancient Greeks, including Hippocrates, routinely used agents with antimicrobial properties such as myrrh and inorganic salts in their treatment of infected wounds (1). The discovery of penicillin by Fleming in 1928, followed by the discovery and clinical use of sulphonamides in the 1930s, heralded the age of modern antibiotherapy (1,2). Penicillin usage became widespread in the 1940s during the war years and by the 1950s the ‘golden era’ of antibiotic development and use was well underway. Probiotics are live microbes that are used as agents to alter the composition or metabolic activities of the microbiota, or to modulate immune system reactivity in a way that benefits health (3,4). The word ‘probiotic’ is also derived from the same Greek term ‘biotikos’ which may be literally translated as ‘for life’. Probiotics have been used for many years in the animal feed industry, but they are now being increasingly made available in many forms and can be purchased over the counter as freeze-dried preparations in health food stores. Prebiotics are food ingredients, usually oligosaccharides, that escape digestion in the upper gastrointestinal tract and selectively stimulate the growth of selective bacterial genera such as bifidobacteria and lactobacilli in the colon (4,5). It is believed that modulation of the normal microflora to benefit the host can be achieved through the use of prebiotics and probiotics. There is now increasing evidence that selected probiotic strains can provide health benefits to their human hosts and it is noteworthy that the Food and Agriculture Organization of the United Nations and the World Health Organization have stated that there is adequate scientific evidence to indicate that there is potential for probiotics to provide health benefits (6). Given these recent developments, it was considered timely to review the background and conceptual framework of the use of these agents and the evidence for their effectiveness in clinical settings. The human intestine contains a complex, dynamic and diverse number of bacteria, that may be differentiated into native inhabitants and transient flora (7). These microorganisms colonize the mucosal surface of the oral cavity, the upper respiratory tract, much of the gastrointestinal tract and the urogenital tract. Although the gastrointestinal tract is sterile at birth, microflora colonize the mucosal surfaces of infants during an ecological succession of organisms that differ from the adult microflora (7). The composition of the flora is influenced heavily by the receipt of oral formula or breast milk. Breastfed infants have been found to have an increased number of Bifidobacteria but rarely have Clostridium species, whereas formulafed infants have large numbers of Lactobacilli, Bacteroides and Clostridium, and relatively few Bifidobacterium species. As solid foods are added to the infant’s diet, the microflora becomes similar regardless of breast feeding status, with Bacteroides and anaerobic Gram-positive cocci appearing in the flora. After the infant reaches two years of age, a conversion to normal adult flora begins, and populations of Bacteroides and anaerobic cocci increase until they equal or exceed those of Bifidobacterium. The number of Gram-negative anaerobes increases to adult levels, whereas coliform, clostridial and streptococcal populations decrease to the levels found in healthy adults. The development of the microflora from the neonatal to the adult composition is very important with respect to the development of the intestinal mucosal immune system and its ability to discriminate between pathogenic microorganisms and the vast array of antigens to which it is exposed over a lifetime. The intestinal mucosa thus has the unique properties of tolerance to environmental antigens (which may include probiotics) and specific immunological responsiveness to mucosal pathogenic microorganisms. The phenomenon of tolerance is thought to occur through clonal deletion, clonal anergy of antigen-specific T cells, or immune deviation medicated via Class I restricted CD4+ T cells and cytokines such as interleukin (IL)-10, or transforming growth factor-β (7).The process is dependent on the normal indigenous flora because germ free animals are defective with respect to tolerance. The immunological response of the gut is mediated through lymphocytes within the lamina propria and both T helper cell 1 (Th-1; IL-12, IFN-γ) and T helper cell-2 (IL-10, IL-4, IL-5) responses

A ntibiotics are used as agents to prevent and treat infec- tions caused by pathogenic bacteria and other microbes, and rank as one of the most important developments of modern medicine.The word 'antibiotic' is derived from the Greek term 'biotikos' and may be literally translated as 'against life'.Antibiotics were first used centuries ago by the Chinese in the form mouldy soybean curd applied to carbuncles and furuncles.
(1) The ancient Greeks, including Hippocrates, routinely used agents with antimicrobial properties such as myrrh and inorganic salts in their treatment of infected wounds (1).The discovery of penicillin by Fleming in 1928, followed by the discovery and clinical use of sulphonamides in the 1930s, heralded the age of modern antibiotherapy (1,2).Penicillin usage became widespread in the 1940s during the war years and by the 1950s the 'golden era' of antibiotic development and use was well underway.
Probiotics are live microbes that are used as agents to alter the composition or metabolic activities of the microbiota, or to modulate immune system reactivity in a way that benefits health (3,4).The word 'probiotic' is also derived from the same Greek term 'biotikos' which may be literally translated as 'for life'.Probiotics have been used for many years in the animal feed industry, but they are now being increasingly made available in many forms and can be purchased over the counter as freeze-dried preparations in health food stores.Prebiotics are food ingredients, usually oligosaccharides, that escape digestion in the upper gastrointestinal tract and selectively stimulate the growth of selective bacterial genera such as bifidobacteria and lactobacilli in the colon (4,5).It is believed that modulation of the normal microflora to benefit the host can be achieved through the use of prebiotics and probiotics.There is now increasing evidence that selected probiotic strains can provide health benefits to their human hosts and it is noteworthy that the Food and Agriculture Organization of the United Nations and the World Health Organization have stated that there is adequate scientific evidence to indicate that there is potential for probiotics to provide health benefits (6).Given these recent developments, it was considered timely to review the background and conceptual framework of the use of these agents and the evidence for their effectiveness in clinical settings.
The human intestine contains a complex, dynamic and diverse number of bacteria, that may be differentiated into native inhabitants and transient flora (7).These microorganisms colonize the mucosal surface of the oral cavity, the upper respiratory tract, much of the gastrointestinal tract and the urogenital tract.Although the gastrointestinal tract is sterile at birth, microflora colonize the mucosal surfaces of infants during an ecological succession of organisms that differ from the adult microflora (7).The composition of the flora is influenced heavily by the receipt of oral formula or breast milk.Breastfed infants have been found to have an increased number of Bifidobacteria but rarely have Clostridium species, whereas formulafed infants have large numbers of Lactobacilli, Bacteroides and Clostridium, and relatively few Bifidobacterium species.As solid foods are added to the infant's diet, the microflora becomes similar regardless of breast feeding status, with Bacteroides and anaerobic Gram-positive cocci appearing in the flora.After the infant reaches two years of age, a conversion to normal adult flora begins, and populations of Bacteroides and anaerobic cocci increase until they equal or exceed those of Bifidobacterium.The number of Gram-negative anaerobes increases to adult levels, whereas coliform, clostridial and streptococcal populations decrease to the levels found in healthy adults.The development of the microflora from the neonatal to the adult composition is very important with respect to the development of the intestinal mucosal immune system and its ability to discriminate between pathogenic microorganisms and the vast array of antigens to which it is exposed over a lifetime.The intestinal mucosa thus has the unique properties of tolerance to environmental antigens (which may include probiotics) and specific immunological responsiveness to mucosal pathogenic microorganisms.The phenomenon of tolerance is thought to occur through clonal deletion, clonal anergy of antigen-specific T cells, or immune deviation medicated via Class I restricted CD4 + T cells and cytokines such as interleukin (IL)-10, or transforming growth factor-β (7).The process is dependent on the normal indigenous flora because germ free animals are defective with respect to tolerance.The immunological response of the gut is mediated through lymphocytes within the lamina propria and both T helper cell 1 (Th-1; IL-12, IFN-γ) and T helper cell-2 (IL-10, IL-4, IL-5) responses ©2004 Pulsus Group Inc.All rights reserved

ADULT INFECTIOUS DISEASE NOTES
may be elicited directed towards intracellular and extracellular pathogens, respectively.In addition, the mucosal microflora have the capacity for inhibitory or bactericidal activity towards transient microbial pathogens within the gut in a process termed microbial interference.
The latter explains one of the postulated mechanisms whereby probiotics exert their protective or therapeutic effects.The beneficial bacteria prevent colonization of pathogenic microorganisms by competitive inhibition for microbial adhesion sites.For example, Lactobacillus casei strain GG and Lactobacillus plantarum have demonstrated the ability to competitively inhibit the attachment of enteropathogenic Escherichia coli (8) and Saccharomyces boulardii has been shown to decrease in vitro attachment of Entamoeba histolytica trophozoites to erythrocytes (9).Another postulated mechanism for the effects of probiotics is the production of organic acids, fatty free acids, ammonia, hydrogen peroxide and bacteriocins, all of which have antimicrobial activity.L casei strain GG produces a low-molecular-weight antibacterial substance that is inhibitory to both Gram-positive and Gram-negative enteric bacteria (10).Another mechanism of probiotic activity is the production of enzymes that modify toxin receptors or block toxin-mediated effects, exemplified by the degradation of Clostridium difficile toxin receptors in the rabbit ileum by S boulardii (11,12).Probiotics may also have significant contributions on intestinal mucosal immunity.Several studies have demonstrated adjuvant-like effects on intestinal and systemic immunity by oral administration of different probiotics, particularly in the stimulation of enhanced immunoglobulin A responses to pathogenic viruses (13)(14)(15) and may also enhance phagocytic activity against intracellular pathogens (16).
The use of probiotics in the form of fermented foods has been commonplace for many years and the benefits of foods containing live bacteria were recognized centuries ago.However, the historical perspective on concepts related to intestinal microecology date back to Elie Metchnikoff (7) in the early part of the 20th century, and he is considered the 'father' of probiotics.Metchinkoff proposed a scientific rationale for the beneficial effects of bacteria in yogurt and attributed the long life of Bulgarian peasants to their intake of yogurt containing Lactobacillus species (7).There are several commercially available supplements containing viable microorganisms with probiotic properties, either in lyophilized form or as fermented food products.The most commonly used probiotics are the lactic acid bacteria including various Lactobacillus, Enterococcus and Bifidobacterium species and nonpathogenic ascospore yeasts, principally S boulardii.
Specific areas of potential use of probiotics that have been proposed in the past 50 years include the prevention and treatment of diarrheal diseases in adults and children, prevention of vaginitis and urinary tract infection in adults, food allergy prevention, and antitumour action in the gut, bladder and cervix.Multiple properties of probiotics have been suggested as potential protective factors in the digestive system against microorganisms such as enteropathogenic E coli, Salmonella, Listeria species and Helicobacter pylori (3,7).However, it is only recently that the scientific knowledge and tools have become available to properly evaluate the effects of probiotics on normal health and well being, and their potential in preventing and treating disease.A recent review of the clinical trials in support of the beneficial effect of probiotics has been published (3).The most supportive evidence of a beneficial effect of probiotics has been established with Lactobacillus rhamnosus GG and Bifidobacterium lactis BB-12 for prevention and Lactobacillus reuteri SD2222 for the treatment of acute diarrhea caused by rotaviruses in children based on randomized, double blinded and placebo-controlled trials.A statistically significant reduction in the duration of diarrhea was reported in several of the trials.A recent metaanalysis evaluated trials on the efficacy of S boulardii and lactobacilli in the prevention and treatment of diarrhea associated with the use of antibiotics and revealed an odds ratio of 0.39 (95% CI, 0.25 to 0.62; P<0.001) and 0.34 (95% CI, 0.19 to 0.61; P<0.01), respectively, in favour of active treatment over placebo (17).Additional clinical trials have suggested a reduction in recurrences of H pylori, alleviation of symptoms of inflammatory bowel disease, carcinogen reduction, allergy reduction, reduction in the occurrence of recurrent urinary infection, and a reduction in recurrences of bacterial vaginosis (3) but the trials were smaller and had less power.The use of probiotics has been advocated by some as a means of reducing or eliminating colonization with antibiotic-resistant microbes (7) and this is another area that requires more study.
Although the use of probiotics may be beneficial in certain settings, one unresolved concern is whether commercially available products matched their claims from both a quantitative and qualitative perspective.Two recent studies, including a Canadian study (18) have suggested that there may be significant differences in the batches of probiotic preparations.The findings match another study that was conducted in Britain (19).These studies serve to highlight the need for better quality control on these products.
The role of probiotics and prebiotics will likely increase in the future as evidence accrues from well conducted studies on the efficacy of these agents when used in standardized and regulated formulations.The potential for probiotics to be used as an adjunct in the control of antibiotic resistance is particularly appealing.