Bacterial Profile and Their Antimicrobial Susceptibility Pattern of Isolates Recovered from Intensive Care Unit Environments at Wachemo University Nigist Ellen Mohammed Memorial Comprehensive Specialized Hospital, Southern Ethiopia

Background Bacterial contamination of indoor hospitals, especially in intensive care units is a serious health hazard in the world with a high morbidity and mortality rates. Particularly, multidrug-resistant bacteria can cross-contaminate medical devices, inanimate surfaces, health care providers, and patients in the intensive care unit. This study was aimed to assess the bacterial profile and their antimicrobial susceptibility patterns of bacterial isolates from intensive care unit at WUNEMMCSH (Wachemo University Nigest Ellen Mohammed Memorial Compressive Specialized Hospital), Southern Ethiopia. Methods A hospital-based cross sectional study was conducted on 180 intensive care unit environmental samples at WUNEMMCSH from August 1, 2022, to October 30, 2022. In this study, a total of 180 swab samples were collected from medical devices, inanimate surfaces, patients, and health care providers from the intensive care unit by using sterile cotton-tipped swabs moistened with normal saline. Then, bacterial isolates were identified using the standard culture method, Gram stain, and biochemical tests. Antimicrobial susceptibility tests for bacterial isolates were performed by using the Kirby Bauer disk diffusion method. Data were entered into EpiData Version 4.6 cleanup and exported to SPSS V25 for analysis. Results A total of 180 swab samples were processed from intensive care unit environments, and 143 (79.4%) were found to have been contaminated by at least one potential pathogenic bacterial isolate. A total of two hundred and thirty-eight bacteria were isolated. Of these, the predominant bacteria were coagulase-negative Staphylococci 72 (30.3%), S. aureus 61 (25.6%), E. coli 41 (17.2%), and K. pneumoniae 30 (12.6%). Seventy (49%) out of all swabbed samples were contaminated with mixed isolates. In the antimicrobial susceptibility tests, 19 (86%) Gram-positive bacteria and 25 (76%) Gram-negative bacterial isolates were susceptible to nitrofurantoin, respectively. Vancomycin was sensitive to 83% of Gram-positive isolates. Gram-positive and Gram-negative isolates from irrespective sources showed multidrug resistance in 56.4% and 76.2%, respectively. Conclusion The inanimate hospital environments, medical device, health care providers, and patients in ICU rooms of WUNEMMCSH (Wachemo University Nigest Ellen Mohammed Memorial Comprehensive Specialized Hospital) were colonized with 143 (79.4%) of potential pathogenic bacterial isolate, which can cause nosocomial infections with high rates of morbidity and mortality among patients. The frequencies of multidrug-resistant 159 (66.8%) bacterial pathogens were alarmingly high. Therefore, to reduce the risk of bacterial contamination and MDR, strict adherence to hospital infection prevention and control measures should be enforced. These measures include regularly performing hand hygiene, periodic disinfection, and sterilization of medical equipment.

Background.Bacterial contamination of indoor hospitals, especially in intensive care units is a serious health hazard in the world with a high morbidity and mortality rates.Particularly, multidrug-resistant bacteria can cross-contaminate medical devices, inanimate surfaces, health care providers, and patients in the intensive care unit.Tis study was aimed to assess the bacterial profle and their antimicrobial susceptibility patterns of bacterial isolates from intensive care unit at WUNEMMCSH (Wachemo University Nigest Ellen Mohammed Memorial Compressive Specialized Hospital), Southern Ethiopia.Methods.A hospital-based cross sectional study was conducted on 180 intensive care unit environmental samples at WUNEMMCSH from August 1, 2022, to October 30, 2022.In this study, a total of 180 swab samples were collected from medical devices, inanimate surfaces, patients, and health care providers from the intensive care unit by using sterile cotton-tipped swabs moistened with normal saline.Ten, bacterial isolates were identifed using the standard culture method, Gram stain, and biochemical tests.Antimicrobial susceptibility tests for bacterial isolates were performed by using the Kirby Bauer disk difusion method.Data were entered into EpiData Version 4.6 cleanup and exported to SPSS V25 for analysis.Results.A total of 180 swab samples were processed from intensive care unit environments, and 143 (79.4%) were found to have been contaminated by at least one potential pathogenic bacterial isolate.A total of two hundred and thirty-eight bacteria were isolated.Of these, the predominant bacteria were coagulasenegative Staphylococci 72 (30.3%), S. aureus 61 (25.6%),E. coli 41 (17.2%), and K. pneumoniae 30 (12.6%).Seventy (49%) out of all swabbed samples were contaminated with mixed isolates.In the antimicrobial susceptibility tests, 19 (86%) Gram-positive bacteria and 25 (76%) Gram-negative bacterial isolates were susceptible to nitrofurantoin, respectively.Vancomycin was sensitive to 83% of Gram-positive isolates.Gram-positive and Gram-negative isolates from irrespective sources showed multidrug resistance in 56.4% and 76.2%, respectively.Conclusion.Te inanimate hospital environments, medical device, health care providers, and patients in ICU rooms of WUNEMMCSH (Wachemo University Nigest Ellen Mohammed Memorial Comprehensive Specialized Hospital) were colonized with 143 (79.4%) of potential pathogenic bacterial isolate, which can cause nosocomial infections with high rates of morbidity and mortality among patients.Te frequencies of multidrug-resistant 159 (66.8%) bacterial pathogens were alarmingly high.Terefore, to reduce the risk of bacterial contamination and MDR, strict adherence to hospital infection prevention and control measures should be enforced.Tese measures include regularly performing hand hygiene, periodic disinfection, and sterilization of medical equipment.

Introduction
Te intensive care unit (ICU) is one of the leading units among health care settings in the percentage of the population with nosocomial infections [1] because of its patients who are susceptible to various infections due to immunodefciency, utilization of invasive medical equipment, administration of various medications, or deformation of normal fora.Health care-associated infection (HCAI) is an infection acquired recently by patients who have been admitted to a health care facility for the purpose of other health care needs.It can happen two to three days after admission or one month or more after release from admission.It results in mortality, morbidity, and expenses, as well as an increase in the hospital stay [2].Infections acquired in intensive care units (ICUs) are a serious health hazard in the world with high morbidity and mortality rates, especially due to crosstransmission of germs [3].
Being frequently coined reservoirs of multidrug resistant (MDR) pathogens, the intensive care unit (ICU) inanimate environment has warranted much attention for its probable contribution to thetransmission of nosocomial infections [4] and may serve as potential reservoirs for nosocomial infection and facilitate transmissions through contact, depending on their tenacity [5,6], and according to the World Health Organization (WHO) [7].Contamination can occur as a result of health care professionals and patient hands or direct shedding of microorganisms that can live for long periods on dry surfaces [8].
Many microorganisms have the potential to cause infections in hospitalized patients [9] because their immunity may be compromised, making them more susceptible to infection.Bacterial pathogens such as coagulase-negative staphylococci (CoNS), Enterobacteriaceae, S. aureus, Pseudomonas spp., Haemophilus infuenzae, Klebsiella pneumoniae, Enterococci, and Acinetobacter spp.are the most common microbe responsible for approximately 90% of HAIs, and they can be found in patients, health care workers, attendants, contaminated instruments, and the environment which are able to survive and persist for long periods of time in the hospital environment [10].Tey also have resistance potential for disinfectants, while protozoa, fungi, and viruses are less attributed to nosocomial infection [11].
Approximately 2 million hospitalized patients in the United States have clinically severe nosocomial infections, resulting in an annual increase in costs [12].Patients who are admitted in the ICU are at a high risk of contracting nosocomial infections as a result of breaches in host defense caused by trauma, invasive medical devices, and/or corticosteroid medication [13].
Te ability of bacteria to develop resistance to antimicrobial agents has made treating bacterial infections more difcult in recent years.Te emergence of resistance to antimicrobial agents is a global public health problem, particularly in pathogens causing nosocomial infections which contributed to the morbidity, mortality, increased health care costs resulting from treatment failures, and longer hospital stays from invasive procedures, high antibiotic usage, and transmission of bacteria among patients due to inadequate infection control measures explain why ICUs are "hot zones" for the spread of antibiotic resistant organisms [14].Also, most of the admitted patients in ICU are treated with empirical prescribed antibiotics, but it commonly leads to antimicrobial resistance (AMR) [15] and/or emergence of multidrug resistance (MDR) as well as death of patients [16,17].Te emergence of MDR strains in the ICU of hospitals, particularly in developing countries, is an increasing problem that is posing a barrier to the management of HCAIs [18] due to high patient fellow, which is a critical role played by the inanimate environment in the transmission of nosocomial infections.Furthermore, antibiotic resistance can be introduced into the ICU invisibly and silently by a commensal member of the patient's or health care personnel's microbiome [19].
Te problem may be further complicated in this study area due to lack of advanced laboratory diagnostics and higher rate of inappropriate empirical antibiotics treatment, cross-contamination between equipment, high patient fow, and health care workers.Te drug susceptibility pattern of the isolates to commonly used antimicrobials in this area will also provide vast options for clinicians to select appropriate antimicrobials for empirical therapy.So this study was aimed at the isolation and antibiotic susceptibility pattern of potentially pathogenic bacterial isolates at ICU environments of the hospital setup.(

Methods
According to information obtained from Wachemo University Nigest Ellen Mohammed Memorial Comprehensive Specialized Hospital, 61 patients were admitted per month on average, and in the three-month period that was estimated to 183 in ICU, the total health care providers were 100 and there were a total of 125 medical devices and 94 inanimate surfaces, respectively, found in the ICU room that were functional during data collection period.For each study individual and environmental surfaces, the sample size was calculated by using proportional allocation by formula, ni � Ni/N * n.Ten, the calculated sample size for medical devices was 43, and for inanimate surfaces 32.By considering a 10% of nonrespondent rates for health care workers and patients, the sample size of health care workers was adjusted to 37, and for patients 68.Hence, the total sample size for the study was 180.

Sampling Techniques and Data Collection.
Te study participants and environmental swab samples were selected from ICU room by convenient sampling technique until the required sample size was achieved.A swab sample was collected from medical devices, inanimate surfaces, hands of patients, and health care providers after 2 hours of disinfection of the ICU rooms at daytime, considering the most representative hours (at 8:00 AM in the morning and 2:00 PM in the afternoon) after a preliminary survey by considering the fact that a higher load of patients with its attendants, clinicians, and medical devices used by them become burden for acquiring microorganisms through close contacting in ICU environments of the hospital and 10 cm 2 regions were swabbed to ensure uniform sampling.Te surface areas of tiny objects or surfaces were approximated and the entire region was swabbed, emphasizing the frequently touched areas in two directions at right angles to each other in a close zigzag pattern at each site, rotating the swab during sampling to ensure that the full surface of the swab is utilized.Most frequently touched part in inanimate or medical device surface swab and most representative locations in each study participants (health care providers and patients) were selected to sample and the sampling sites were categorized into four groups: (1) commonly touched medical devices including stethoscopes (ear piece, tubing, and diagram), sphygmomanometer (bulb and cuf), body incubator (sides and top), suction machine (tubes and needle), weighing scale (sides and top), and thermometer NICU (mercury and tubes).( 2 ), and chair (sides of chair).(3) Hands of patients (fngers and palm area of the hands).( 4) Hands of health care providers (fngers and palm area of the hands) [21].

Sample Collection and Transportation.
Self-contamination was prevented by wearing sterile disposable gloves, mouth masks, and protective gown.Te swab samples were employed to collect from inanimate, medical device, and study individuals.Prelabeled sterile cotton swab sticks were moistened in 0.85% sterile normal saline and were rolled over the medical devices and inanimate surfaces, hands of patients, and health care providers (each 10 cm 2 surface area) [21].Each swab was aseptically replaced into a test tube containing 10 ml of normal saline and sealed.Ten, they were properly labeled and all collected specimens were transported to the microbiology laboratory of Southern Nations, Nationalities, and People's Region Health Bureau, Hosanna Branch Regional Laboratory Institute within 30 minutes to one hour, and the solution containing the swab was thoroughly agitated with a vortex mixer to release the bacteria from the swab.Ten, all swab samples from the pieces of equipment and each study individuals were analyzed by clinical laboratory standard protocols [22].
Canadian Journal of Infectious Diseases and Medical Microbiology

Processing of Specimens and Preliminary Identifcation.
Isolation and identifcation of bacteria were performed by clinical microbiology techniques such as the culture method, Gram stain, and biochemical tests.A 100 μl of the all diluted sample was aseptically inoculated using a sterile spreader onto each of 5 percent sheep blood agar plates (Oxoid Ltd., UK) for both Gram-negative and Gram-positive bacterial isolates, MacConkey agar (Oxoid Ltd., UK) for Gramnegative bacterial isolates, and mannitol salt agar (Oxoid Ltd., UK) for identifcation of Staphylococcus species.Te inoculated plates were incubated at 37 °C for 24-48 hours and observed for any bacterial growth.Subcultures of the respective bacterial isolates were subsequently subjected to species identifcation and confrmation for the initial screening of suspected pathogens and an estimation of the colony characteristics.Te grown colonies' characteristics of isolated bacteria have been identifed by Gram staining and standard biochemical tests.Gram-positive cocci were identifed by Gram staining from blood agar culture media.Also, they were identifed using catalase and coagulase tests from mannitol salt agar.Members of Enterobacteriaceae family were identifed by a series of biochemical tests including the following: catalase, indole, citrate, motility, urease, H2S production, and oxidase test and triple-sugar iron.Nonlactose fermenting Gram-negative bacteria were identifed by indole, triple-sugar iron, urease, oxidase, motility, and catalase tests.If there was no sign of growth after 48 hrs of incubation, the sample was reported to be culture negative [22].

Antimicrobial Susceptibility
Testing.Antibiotics were selected based on local availability, information from literature, and efectiveness.Antimicrobial susceptibility patterns of the bacterial isolate were tested using the modifed Kirby-Bauer disk difusion method on the Mueller-Hinton agar (MHA) (Oxoid Ltd., UK) according to the Clinical Laboratory Standard Institute guidelines [23].Te colonies were emulsifed in a sterile normal saline solution, and densities of suspension were compared with the opacity standard on a 0.5 McFarland solution to give a density equivalent to that of the standards [24].A sterile swab was dipped into the suspension of the isolate in broth; excess fuid was removed against the side of the bottle.Te entire surface of the MHA plate was swabbed with the test organism suspension, turning the plate 360 degrees and repeating the process three times.Te antimicrobial disks were placed on the surface of the agar and gently pressed down with sterile forceps.Ten, the medium was incubated at 37 °C for 18-24 hours.Te results were reported as sensitive, moderate, or resistant using the Clinical and Laboratory Standards Institute's (CLSI) breakpoints [25].Antibiotic disks containing methicillin 5 μg, cloxacillin 5 μg, nitrofurantoin 300 μg, tetracycline 30 μg, cefoxitin 30 μg, gentamicin 30 μg, azithromycin 15 μg, cefxime 5 μg, ciprofoxacin 5 μg, clindamycin 10 μg, chloramphenicol 30 μg, vancomycin 30 μg, meropenem 10 μg, ceftazidime 30 μg, cotrimoxazole 25 μg, ceftriaxone 30 μg, ampicillin 10 μg, and amoxicillin/clavulanic acid 10 μg were used [26].From the Gram-negative isolates, E. coli 41 (39%) were the most predominant bacteria followed by K. pneumoniae 30 (28.6%), P. aeruginosa 13 (12.4%), A. baumannii 11 (10.5%),Enterobacter spp.6 (5.7%) and Salmonella spp. 4 (3.8%).Te details of the isolates are shown in Table 1.

Proportions of Bacterial Isolates in ICU Environments.
From the total 180 swab samples, the highest bacterial contamination rate was observed in inanimate surfaces 28 (87.5%) and medical devices 37 (86%), followed by patients 54 (79.4%) and HCPs (health care providers) 24 (65%).Te positive rates of each study participants are summarized in Table 2.

Discussion
Tis study was aimed to assess the bacterial profle from the intensive care unit at WUNEMMCSH (Wachemo University Nigest Ellen Mohammed Memorial Compressive Specialized Hospital), Southern Ethiopia, and evaluate their antimicrobial susceptibility patterns.Evidence-based knowledge about the extent of contamination of the hospital environment is important for designing and implementing efective prevention and control measure to tackle hospital-acquired infections.Moreover, the study results may give an insight for health professionals.While intensive care units are expected to be clean, they are usually reservoirs of common hospital-acquired bacterial pathogens and multidrug-resistant bacterial isolates.Te output of this study showed that various intensive care unit environments were directly or indirectly contaminated by eight potential pathogenic bacterial isolates such as CoNS, S. aureus, E. coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, A. baumannii, Enterobacter, and Salmonella spp. that raise serious concerns about possible nosocomial transmission.In   In this study out of 180 swab, 143 (79.4%) of the various study swabs were contaminated with bacterial isolates, from which 86.7% of medical devices and inanimate surfaces were positive for at least one isolate.Tis is relatively in agreement with other study results, from medical devices and inanimate surfaces in Bahir Dar city 87.7% [20], Mekelle, Ethiopia 88.5% [27], and Zimbabwe 86.2% [4].Tis fnding contradicts with that of the studies conducted elsewhere due to lower bacterial contaminations observed in India being 54.4% [28], in Arba Minch 71.7% [29], and due to higher bacterial contaminations observed in studies conducted elsewhere in Windhoek, Namibia, which is 95% [30].Tese deviations may be due to variation in study time and location, poor infection control and hand hygiene practices, intensive care unit room ventilation system, sterilization, procedures in disinfection, and sampling techniques.Similarly, in this study, the highest bacterial contaminate and multibacterial isolates were identifed from patient beds, sphygmomanometer, stethoscope, body incubator, and others.Tis is in agreement with other studies conducted in Iran [3], Mekelle, Ethiopia [27], and Bahir Dar city [20].Also, from the current study, 64.8% of health care providers' hands were contaminated with bacteria.Our result relatively agreed with that of other reports where bacterial contamination was found to be 64.5% in Iran [3].It contrasts with current study, and lower bacterial contaminations were observed in India 17.8% [28] and Namibia 5% of HCP [30].Tese variations may be due to wearing single personal protective gowns by many staf, investigating patients without gloves, poor infection prevention and hand-washing practice of patients, poor disinfection practice for medical devices and closely contacted inanimate patient surfaces before and after patient examinations, and higher bacterial isolates were identifed from studies in Zimbabwe (100%) [31] due to unregular cleaning of hands with antiseptics and not disinfecting the most touched surfaces of medical equipment and inanimate surfaces.In this study, 79.4% of patients were contaminated with at least one bacterium isolates.It is relatively agreed with the study conducted in Tanzania 88.5% in patients [32].
Te results of our study showed that varied groups of bacteria, including both Gram-positive (56%) and Gramnegative (44%) bacteria contaminated the ICU environments.Comparable to our results, the frequency of Grampositive bacteria constituted the leading inhabited bacteria compared to Gram-negative bacteria in diferent countries, for example, in Iran (60.7% vs. 39.3%)[33], Nigeria (52.2% vs. 47.8%)[28], and Gondar in Ethiopia (60.5% vs. 39.5%)[34].Te dominance of Gram-positive over Gram-negative bacteria could be described by the fact that Gram-positive bacteria, being free of lipid-dominant drying up prone outer membrane, have concerning nature ability to keep their viability on diferent hospital environments for several time periods [33].In contrast to our results, the study conducted from Addis Ababa in Ethiopia reported that Gram-negative bacteria were predominant than Gram-positive ones (82.6%, 17.4%) [10] and in Zimbabwe (66.2% vs. 33.82%,respectively) [4], and these variations may be due to diferent sampling times (endemic vs outbreak situations), the presence of colonized and/or infected patients during sampling, and the use of diferent sampling techniques and culture methodologies.
P. aeruginosa (12.4%) was the third-reported GNB, and it was almost similar to the fndings of the study conducted in Hiwot Fana hospital, Ethiopia 7.3% [41], Gondar, Ethiopia 11.4% [34], and Mizan-Tepi, Ethiopia 11.4% [36].It contradicts with that of the study in conducted in Mekelle 1.3% [27].Tis diference might be due to the primary distribution of microorganism to hospital environment, and it is also particularly well-adapted to live in wet or moist conditions.In this study, 10.5% Acinetobacter baumannii was identifed.Comparable results were found in studies conducted in Addis Ababa, Ethiopia, in which 17.4% of Acinetobacter baumannii were found [10], and in Iran (8.3%) [43] and Windhoek, Namibia, (5.3%) [30].Salmonella species 4 (3.8%) is potentially pathogenic isolates which was identifed from medical devices and inanimate surfaces.It is similar with the fndings of the study conducted in Mekelle (3.5%) [27] and Hiwot Fana (7.3%) [41], but it is lower than that of the study conducted in Arba Minch (23.3%) [29].Te presence of these microorganisms might be due to the aspiration of secretions in colonized patients in the unit or poor hand hygiene practices and contaminated equipment/ inanimate surface.
In this study, E. coli 39% and P. aeruginosa 92% were resistant to tetracycline, which is in line with similar resistance rates E. coli 50% and P. aeruginosa 83.3% from the study conducted in Hawassa [44].Also, in these studies, vancomycin (83%) was the most sensitive tested antibiotic for Gram-positive bacterial isolates, which is similar to that of the study conducted in Zimbabwe (78%) [4] but contradicts with that of the study for resistance to vancomycin (100% in India [3].CoNS and S. aureus were mostly resistant to ampicillin (67%), cloxacillin (59%), and cotrimoxazole (67%) which was similar to the fndings of the studies conducted in Nigeria [28] and Bahir Dar [45].Also, in this study, S. aureus was resistant to cotrimoxazole (69%) and ciprofoxacin (54%) but most sensitive to gentamicin (76%).Tis fnding is relatively similar with resistance to cotrimoxazole (79%) and ciprofoxacin (50%) but sensitive to gentamycin (96%) in the study in Sudan [38].Our results showed that 159 (66.8%) bacterial isolates were resistant to at least 3 antibiotics.Multidrug resistance was detected both in 56.4% of Gram-positive and 76.2% of Gram-negative isolates, which was in line with the fndings of other studies, which were conducted in Sudan (84.5%) [38], Bahir (57.5%) [20], and Hawassa (57.7%) [44].

Limitation of the Study.
Te study has a number of advantages.It gives useful information about the bacterial profle and antimicrobial susceptibility trends of potential pathogenic bacteria in hospital settings, especially in ICU rooms.However, the study does have the following drawbacks: (i) It was carried out in a single hospital, which might not be an accurate representation of other hospitals in the area (ii) Anaerobic and/or fastidiously growing bacteria in this study were not investigated because the isolation of these requires special procedures and equipment that were not available (iii) Other microbial contaminants, such as parasites, virus, molds, and yeasts, which were beyond the scope of this study, were not investigated (iv) Moreover, settle plate samples were not taken from the ICU environment of the hospitals and samples were taken once from each selected study participants 10 Canadian Journal of Infectious Diseases and Medical Microbiology

Conclusion and Recommendations
Te inanimate hospital environments, medical device, health care providers, and patients in the ICU rooms of WUNEMMCSH (Wachemo University Nigest Ellen Mohammed Memorial Comprehensive Specialized Hospital) were colonized with 143 (79.4%) potential pathogenic bacterial isolates, which can cause nosocomial infections with high rates of morbidity and mortality among patients.CoNS were the predominant bacterial type, followed by S. aureus and E. coli.Nitrofurantoin was the most sensitive drug for all isolates; similarly gentamicin and vancomycin was the most efective antibiotics for all Gram-negative and Gram-positive isolates, respectively.Te frequency of MDR bacterial pathogens was found to be 159 (66.8%), which was alarmingly high.Tis might be a refection of inappropriate use of antibiotics or unavailability of a guideline regarding the selection of drugs.Te presence of MDR in ICU environments may be a predisposing factor for infection.Terefore, to reduce the risk of bacterial contamination and MDR, strict adherence to hospital infection prevention and control measures should be enforced.Tese measures include performing regularly hand hygiene, periodic disinfection, and sterilization of medical equipment.
) Commonly touched inanimate surfaces including patient bedside surfaces (bedside table bottom and bedside table top), wall surfaces (close contact to patient), door handle, table (top and sides of table Canadian Journal of Infectious Diseases and Medical Microbiology were secured and coded to maintain confdentially.Disclosure of laboratory fndings to Wachemo University Nigest Ellen Mohammed Memorial Comprehensive Specialized Hospital was made. 2.9.Data Quality Control.Te reliability of the study results was guaranteed by implementing quality control (QC) measures throughout the whole processes of the laboratory works.Aseptic techniques were observed in all the steps of specimen collection and inoculation onto culture media to minimize contamination.All culture media have been prepared according to the criteria of the manufacturers.Culture media were tested for sterility and performance.Te standard operating procedures (SOPs) were strictly followed during each laboratory work.Control bacteria strains such as Escherichia coli (ATCC 25922), S. aureus (ATCC 25923), and Pseudomonas aeruginosa (ATCC 27853) were used in controlling the potency of the drugs.cialized Hospital.Permission was obtained from the hospital to conduct the study.For data collection, informed consent was obtained from health care providers and admitted patients after explaining the purpose and procedure of the study.All information obtained from the study participants 4 Gram-negative bacteria.Seventy (49%) swabbed samples had multibacterial contaminations, of which S. aureus plus CoNS accounted for 13 (18.6%),S. aureus plus K. pneumoniae accounted for 8 (11.4%), S. aureus plus E. coli accounted for 7 (10%), CoNS plus K. pneumoniae accounted for 6 (9%), and CoNS plus E. coli accounted for 5 (7%).CoNS 72 (54.14%) and S. aureus 61 (45.86%) were predominant Gram-positive bacteria isolated from the swab samples in ICU.

Table 2 :
Proportion of bacterial isolates from diferent study groups in ICU environments at Wachemo University Nigist Ellen Mohammed Memorial Comprehensive Specialized Hospital, August 1, 2022, to October 30, 2022

Table 3 :
Antimicrobial resistance profle of bacterial isolates from ICU environments at Wachemo University Nigist Ellen Mohammed Memorial Comprehensive Specialized Hospital,