The Significance of PAX8-PPARγ Expression in Thyroid Cancer and the Application of a PAX8-PPARγ-Targeted Ultrasound Contrast Agent in the Early Diagnosis of Thyroid Cancer

Objective To investigate the significance of PAX8-PPARγ expression in thyroid cancer and the application of a PAX8-PPARγ-targeted ultrasound contrast agent in the early diagnosis of thyroid cancer. Methods In this study, the expression of PAX8-PPARγ in thyroid cancer tissues, paracancer groups, and normal thyroid tissues was detected by western and immunohistochemical techniques; the effects of PAX8-PPARγ expression inhibition on thyroid cancer cell growth, clonogenic ability, and antiapoptosis were examined. The terminal carboxylactic acid/hydroxyacetic acid copolymer (PLGA-COOH) nanoparticles were prepared by the double emulsification solvent volatilization method. The in vitro cytotoxicity of the targeted contrast agent was detected by MTS and other methods; LD50 was used to evaluate its short-term in vivo toxicity after intraperitoneal injection in mice. Results PAX8-PPARγ expression was significantly increased in thyroid cancer tissues, and the expression level of PAX8-PPARγ was closely correlated with TNM staging and lymph node metastasis (P < 0.05). In addition, PAX8-PPARγ was also expressed at high levels in thyroid cancer cell lines relative to normal thyroid cells. MTS experiments showed that the PAX8-PPARγ-targeted ultrasound nanocontrast agent had no significant toxic side effects on thyroid cells; countess observed that the contrast agent had no effect on cell survival and mortality; the LD50 assay showed that the targeted contrast agent had a wide safety range. Western blot showed the expression of caspase-3, BAX, and Bcl-2 in thyroid cancer cells, indicating that the nanocontrast agent has a good biosafety. In vitro targeting experiments showed that there were more nanospheres aggregated around the cells in the targeted contrast group. In vivo targeting imaging of nude mice revealed that the ultrasound signal was significantly enhanced in the targeted group compared with the nontargeted group after 20 min of LIFU irradiation. Conclusion PAX8-PPARγ overexpression in thyroid cancer cell lines and thyroid cancer tissues promoted the proliferation and antiapoptotic ability of thyroid cancer cells and promoted the tumorigenic ability in nude mice in vivo. We successfully prepared a PAX8-PPARγ-targeted ultrasound nanocontrast agent, which has regular morphology, uniform size, and high stability, and its liquid-gas phase change can be promoted at lower temperature. Therefore, this contrast agent can achieve US-targeted imaging and temperature phase transition function, and may have enhanced ultrasound imaging potential.


Introduction
yroid cancer is the most common malignancy of the endocrine system and although its mortality rate is low, especially in stage I or II of tumor development, the 10-year survival rate of patients is higher than 98%; however, it is of concern that when the tumor is detected in a more advanced stage or when distant metastases are present, it can reduce the survival rate to 56% [1,2]. Currently, the clinical diagnosis of thyroid tumors is still based on pathological diagnosis as the gold standard, but surgical resection treatment is an invasive test. Ultrasound-guided thyroid aspiration cytology, which is widely used in clinical practice, has partially reduced the invasiveness, but many clinical challenges are often encountered, such as small tumors that are difficult to get to the location of the tumor, small amounts of tissue or tissue damage that makes pathology difficult to make a correct diagnosis, and surgical biopsy is an absolute contraindication for patients with bleeding tendency [3]. erefore, it is important to study new methods for noninvasive and targeted diagnosis of thyroid tumors.
In recent years, with the continuous research in the ultrasound neighborhood, drug-or gene-loaded microbubbles have been successfully prepared, which can be coupled with monoclonal antibodies to nanoparticles or microbubbles by various methods for targeted contrast, for drug-and gene-loaded purposes, providing new ideas for the diagnosis of malignant tumors [4,5]. Leong-Poi et al. showed that targeted nanobubble contrast agents have good specificity by coupling specific antibodies on the surface of lipid microbubbles to achieve targeted visualization of the tumor vessels [6,7].
us, molecular imaging based on targeted nanobubbles brings a new hope for the early noninvasive diagnosis of tumors and has become a hot topic of research in the biomedical neighborhood in recent years.
It has been shown that the coding region of the paired cassette gene, chromosome 2q13, is fused to the coding region of the peroxisome proliferator-activated receptor c, 3q13, to form a new gene, PAX8-PPARc, which is characteristically expressed in follicular thyroid cancer (FTC) and also in other thyroid tumor tissue types; its positive expression rate varies among different ethnic and geographical populations. e positive rate of expression also varies among different ethnic and geographical groups [8,9].
In recent years, new methods based on mutations and other molecular markers have been partially applied in differential diagnosis of thyroid tumors (e.g., BRAF, TP53, CTNNB1, and other genes), and these molecular markers have significantly improved the diagnostic accuracy of cytology and have had a profound impact on the classification and management of patients with thyroid tumors. However, the application of nanotechnology combined with specific antibody targeting visualization technology in non-invasive imaging diagnosis of thyroid cancer has not been reported yet. Since our previous experiments confirmed that PAX8-PPARc was expressed in solid tumor tissues of thyroid cancer and had an impact on its malignant biological behavior both in vivo and ex vivo, this would potentially provide a new molecular tumor marker for thyroid cancer and provide the necessary experimental basis for the next quantitative assessment of PAX8-PPARc aggregation in thyroid cancer by molecular imaging methods in vivo. erefore, we envision that a breakthrough point needs to be found for the early noninvasive diagnosis of thyroid cancer with PAX8-PPARc coupled with an ultrasound nanobubble contrast agent.
Based on the above theories, this project proposes to prepare PAX8-PPARc-targeted ultrasound contrast agents, and it is our main goal to explore new methods based on nanomicrobubble targeting to mediate thyroid tumor diagnosis using the constructed cellular and animal models. By coupling the thyroid-specific recognition antibody PAX8-PPARc and magnetic materials on the nanomicrobubbles, we can target PAX8-PPARc on the cell membrane surface of thyroid cancer cells and target PAX8-PPARc on the animal model. PPARc and in vivo tracing of tumors in animals, which increases the sensitivity of ultrasound imaging of thyroid tumors and can confirm the microscopic pathological basis of thyroid cancer lesions based on the anti-PAX8-PPARc-targeted polymeric contrast agent, improves the early, non-invasive, and accurate diagnosis of thyroid cancer, and helps to explore the next step of molecularly targeted therapy for thyroid cancer based on it.
In summary, this project can provide a new theoretical basis and drug targets for the clinical targeting treatment of thyroid cancer through the development of a novel, efficient, and specific nanotargeted ultrasound contrast agent with clinical application prospects, which can diagnose thyroid cancer noninvasively and early, and has not been reported in the literature at home and abroad.

Research Subjects.
Sixty-five cases of surgically resected TC tissue and corresponding paracancerous tissue (5 cm from the edge of the cancer foci) were collected from May 2018 to July 2019 in our thyroid surgery, and the patients' ages ranged from 22 to 71 years, with a median age of 44 years; 18 of them were male and 47 were female. All patients were diagnosed with primary thyroid cancer preoperatively.
Patients who had not yet undergone systemic antitumor therapy and who were included or their families signed the informed consent form for this study.

Expression Levels of PAX8-PPARc mRNA in Tissues and
Cells by qPCR. RNAiso Plus was used to extract total RNA from tissues or cells, and the concentration of RNA was measured spectrophotometrically. 1 μg of RNA was diluted to 1 ml of nuclease-free water and used as a template for reverse transcription. Primer sequences were as follows: PAX8-PPARc forward: 5′-TTCGATGTCTCTAGGGTGG C-3′ and reverse: 5′-GATCCACCTCTCTCGCTCTCT-CAG-3′. 10 μl of the SYBR Green nucleic acid fluorescent dye system was used for the qPCR, and three parallel wells were set up, after predenaturation at 95°C for 10 min, denaturation at 95°C for 15 s, annealing at 60°C for 30 s, extension at 72°C for 45 s, and 40 cycles. e expression levels of UCHL5 and BRAF mRNA in tissues and cells were calculated by the 2-ΔΔCT method using β-actin as the internal reference gene.

WB Detection of UCHL5 and BRAF Protein Expression Levels in TC Tissues and Cells.
e total protein of TC tissues and cells was extracted and the BCA protein quantification kit was used to detect protein concentration, take 40 μg protein line, 10% SDS-PAGE, 120 V constant pressure for 2 h, 260 mA constant flow wet transfer to the PVDF membrane, Add UCHL5 and BRAF antibodies (1 : 1 000) and incubate it overnight in a refrigerator at 4°. e film was exposed to the ChemiDoc XRS + imaging system (Bio-Rad, USA), and the grayscale values of the bands and the relative protein expression were calculated using ImagePlus.

NPs-PAX8-PPARc General Properties Assay
(1) Contrast agent morphology and distribution were observed under light microscopy (2) Morphology and distribution under scanning electron microscopy (3) Measurement of nanoparticle size and a zeta potential analyzer to detect its particle size

Flow Cytometry Detection of Binding
Rate. e other one was incubated with a fluorescently labeled secondary antibody for 30 min and was mixed every 10 mins by using a gun tip, and then the binding rate was detected by flow cytometry in each group.

NPs-PAX8-PPARc In Vitro ermal Phase Change Experiments
(1) Adjust the heating plate put in position, so that the microscope observation is simple and easy to operate (2) Several drops of nanoparticles were placed on the heating plate (3) e heating plate temperature was set to 60°C

In Vitro Experiments on Ultrasound-Induced NPs-PAX8-PPARc Phase Transition and Ultrasound
Development. Nanospheres of NPs and phase-change targeting nanospheres of NPs-PAX8-PPARc were added to preprepared agarose gel wells and irradiated in advance with LIFU (acoustic power 1.60 w/cm for 1 min). Images were acquired before and after the irradiation.

Targeted Ultrasound Contrast Agent Cytotoxicity Test.
Nthy-ori3-1 cells were obtained at the logarithmic growth stage. e cells were routinely digested into cell suspensions and the cell concentration was adjusted to 5 × 103 cells in 96-well culture plates and incubated for 24 hours at 37°C and 5% CO2. Different concentrations of nanocontrast agent nanoparticles were added in 4 gradients (5 pmol/mL, 10 pmol/mL, 15 pmol/mL, and 20 pmol/mL), and a negative control and a positive control were set up. After incubation for 48 h, the liquid in the wells was discarded and 10 μL/well of MTS was added, and the light absorption values were measured after incubation at 37°C for 3 h. e data were statistically analyzed and plotted using Excel software.

In Vitro Cell Targeting Experiments.
(1) Cell membranes were stained by adding 0.05 mg/ml DiO per cell crawl in each well of a six-well plate. (2) e stained cells were incubated for 30 min, and then the culture medium was drained and washed three times with PBS. (3) 150 μL of the targeted polymer contrast agent (labeled with Dil) was added to each of the three wells, and 150 μL of the normal polymer contrast agent (labeled with Dil) was added to each of the other three wells. (4) e cells were incubated in the incubator for 1 h, then each coverslip was washed with PBS and the cells were fixed; the binding of the contrast agent to the cells was observed by light microscopy.

Establishment of Nude Mouse
Model. SW579 cells at the logarithmic growth stage were digested with conventional trypsin and resuspended in a serum-free medium. e cells were then centrifuged, resuspended in PBS and placed on ice, and inoculated on the right side of the back of nude mice under the skin for about 4 weeks for subsequent experiments.

In Vivo Observation of the Target-Seeking Ability of Targeted Contrast Agents in Animal Experiments
2.9.1. Experimental Grouping. Five mice from each of the established PAX8-PPARc SW579 nude mouse models were used and grouped according to the type of the intravenous contrast agent injected into the following two groups; Group I (model + normal contrast group), n � 5 Group II (model + dual-modality-targeted contrast group), n � 5

Ultrasonic
Imaging. e Vevo 770 UBM small animal ultrasound scanning system (VisualSonics Inc., Toronto, Canada) was used for tumor imaging and analysis. After the injection of various types of ultrasonic microbubble contrast agents into the tail vein of the rats, LIFU was used for irradiation (1.40 w/cm 2 for 20 min) to observe the changes in the echogenic intensity of the tumors after the injection of different contrast agents.
2.11. Statistical Processing. SPSS23.0 statistical software was used for analysis, and the normally distributed measurement data were expressed as x ± s, and count data were expressed as percentages. e t-test was used for comparison between groups for measurement data, and χ 2 test was used for comparison between groups for count data, with P < 0.05 or Contrast Media & Molecular Imaging P < 0.01 indicating that the differences were statistically significant.
e results showed that PAX8-PPARc was highly expressed in thyroid cancer cell lines ( Figure 1) compared to normal thyroid cell lines (Figures 1(a) and 1(b)). Immunohistochemistry and western blotting showed that PAX8-PPARc was significantly highly expressed in thyroid cancer tissues compared to normal thyroid tissues (P < 0.001; Figures 1(c)-1(e)). � 0.8994; Table 1).

PAX8-PPARc Expression Was Positively Correlated with the Degree of yroid Tumor Differentiation and TNM Staging.
All thyroid cancer specimens were analyzed by immunohistochemistry. PAX8-PPARc expression was not found to differ between genders. High PAX8-PPARc expression was associated with thyroid tumor differentiation, TNM staging, and lymph node metastasis (P < 0.05; Tables 1  and 2). erefore, it is speculated that PAX8-PPARc may have a positive role in thyroid tumor initiation and progression.

Effect of Inhibiting PAX8-PPARc Expression on the Proliferation Ability of yroid Cancer Cells.
yroid cancer cells were transfected with a PAX8-PPARc antisense oligonucleotide siRNAPAX8-PPARc to block the expression of PAX8-PPARc, and the effect of blocking on the clonal growth of thyroid cancer cells was examined and evaluated. e SW579 thyroid cancer cell line is a PAX8-PPARc highexpression cell, and after treating the cells with different concentrations of the PAX8-PPARc antisense oligonucleotide, the change of PAX8-PPARc expression in the treated cells was detected by western blot. After treatment with different concentrations of the PAX8-PPARc antisense oligonucleotide, the PAX8-PPARc expression changes in the treated cells were detected by western blot. As shown in

Effect of Inhibition of PAX8-PPARc Expression on the Tumorigenic Ability in Nude Mice
In Vivo. Compared with the control group, the tumorigenic ability of SW579 thyroid cancer cell in nude mice was significantly inhibited after PAX8-PPARc expression was blocked by interference. When nude mice were executed on the 50th day of inoculation, tumor formation was found in the nude mice with undisturbed PAX8-PPARc expression, and the tumor weight and growth curve are shown in Figures 3(a) and 3(b).

Preparation and Characterization of the NPs-PAX8-PPARc Contrast Agent
(1) e NPs-PAX8-PPARc contrast agent was observed as a milky white suspension after preparation and could be stored stably at 4°C. e contrast agent prepared by adding DiI dye in advance was a red suspension (Figure 4(a)).
(2) e prepared nanocontrast agent was uniform in size and well dispersed as observed by using an ordinary light microscope (Figure 4(b)) (3) e average particle size of NPs-PAX8-PPARc was 535.7.2 ± 40.7 nm (Figure 4(c)) and the average surface potential was −14.23 ± 8.69 mV (Figure 4(d)) 3.5.1. Flow Cytometry Detection of Binding Rate. e PAX8-PPARc antibody was bound to the nontargeted nanocontrast agent and flow cytometric detection showed 67.2% binding of the targeted nanocontrast agent to the secondary antibody ( Figure 5(b)) and 0.02% binding of the nontargeted nanocontrast agent to the secondary antibody, as shown in Figure 5(a).

In Vitro ermal Phase Change Experiments of NPs-PAX8-PPARc
(1) e phase transformation of NPs-PAX8-PPARc in the heated plate was observed by ordinary light microscopy ( Figure 6). (2) When the temperature of the heating plate increased, the nanoparticles began to show changes, and when the temperature reached 45.9°C, bubbles began to be generated. When the temperature rises to 47°C, more bubbles were gradually produced.

Ultrasound-Targeted Contrast Agent in In Vitro Phase
Change and Contrast Development Experiments. Before irradiation with the LIFU instrument, the nanocontrast agent exhibited no echo in the ultrasound mode (mechanical index 0.08). After irradiation with the LIFU instrument, the targeted nanocontrast agent solution exhibited uniform strong echoes in the form of fine dots (Figure 7).

MTS Method to Detect the Effect of Targeted Contrast
Agents on the Proliferative Capacity of yroid Cells. e normal thyroid cell lines were treated with different concentrations of nanocontrast agents, and four gradients of nanoparticles (5 pmol/mL, 10 pmol/mL, 15 pmol/mL, and 20 pmol/mL) were set up in this experiment, with negative and positive controls, respectively. e effect of receiving targeted nanocontrast agent treatment on the proliferation ability of normal thyroid cell lines was observed by the MTS method. As shown in Figure 8, there was no significant change in the growth and proliferation trend of thyroid Nth-ori3-1 cells between the groups treated with the nanocontrast agent and the blank control group, and the growth and proliferation ability of the positive control group treated with Adriamycin was significantly inhibited, which suggested that the targeted nanocontrast agent had no significant effect on the survival and proliferation of thyroid cancer cells. Four gradients (nanoparticle concentrations of 5 pmol/mL, 10 pmol/mL, 15 pmol/mL, and 20 pmol/mL, respectively) were set up in this set of experiments, with negative and positive controls, respectively. e nuclei of the cells were stained with DAPI to observe the effect of the targeted contrast agent on the morphology of the nuclei to reflect the cytotoxicity of the targeted contrast agent, as shown in Figure 9; the cells in the Adriamycin-treated group, which showed nuclear fragmentation, while the nuclei of the blank control and nanotargeted contrast agent groups remained intact and unaffected, which to some extent indicates that the targeted nanocontrast agent made in this study has in vitro cell safety.

Countess Observation of Cell Survival and Mortality.
Cells were treated with different concentrations of nanocontrast agents for 72 h. Four gradients (5 pmol/mL, 10 pmol/mL, 15 pmol/mL, and 20 pmol/mL) of nanoparticles were set up in this experiment, with negative and positive controls, respectively. e cells were stained with TissueBlue to observe the effect of targeting contrast agent treatment on the viability of the cells. As shown in Figure 10, there was no significant effect on cell viability in the blank control and nanotargeted contrast agent groups, while the number of dead cells in the Adriamycin-treated group was significantly increased.

LD50 Measurement Results.
After intraperitoneal injection of different doses of targeted nanocontrast suspensions, the animals were observed daily until 15 d after administration. e mice in each experimental group did not show any death within 15 days of injection, and their body weight increased normally (Table 3).

Western Blot Detection of Caspase-3, BAX, and Bcl-2 Expression in yroid Cancer Cells.
e thyroid cancer cell lines were treated with 1640, nontargeted contrast agent (20 pmol/mL), targeted contrast agent (20 pmol/mL), and adriamycin (0.5 umol/mL) for 72 hours, and then the effects on caspase-3, BAX, and Bcl-2 expression were observed. As shown in Figure 11, the expression of caspase-3 and BAX was significantly higher in the adriamycin-treated cells compared with the blank control group and the targeted and nontargeted contrast agent-treated cells. In contrast, the expression of Bcl-2 protein was significantly lower in adriamycin-treated cells compared to the other three experimental groups. ese results suggest that the targeted nanocontrast agent had no effect on cell growth and apoptosis.

Nude Rat Model Establishment.
e animal model used in this experiment was a nude mouse, and 4 weeks after inoculation of tumor cells on the right side of the back, the tumor was successfully constructed by subcutaneous transplantation (Figure 12); the size of the tumor was about 1.0 cm × 0.8 cm × 0.9 cm, ≤1 cm, measured under ultrasound.

In Vitro Cell Targeting Assay.
e prepared NPs-PAX8-PPARc and NPs-control nanoparticles were coincubated with SW579 cells. e results showed that the number of nanoparticles attached per cell was significantly (P < 0.001) higher in the targeted contrast agent group (NPs-PAX8-PPARc) than in the nontargeted contrast agent group (NPscontrol), as shown in Figure 13.

In Vivo Target Finding Experiments in Nude Mice.
e ultrasound enhancement imaging results showed that the ultrasound signal of the tumor in the tumor region was significantly increased in the targeted ultrasound contrast agent group (48.32 ± 2.9 a.u) and in the nontargeted ultrasound contrast agent group (6.03 ± 1.6 a.u). e ultrasound signal was also enhanced in the tumor region of the nontargeted contrast group (6.03 ± 1.6 a.u), but the signal enhancement was significantly weaker than that of the targeted group, as shown in Figure 14.
Different ultrasound imaging modes were used following the injection of PAX8-PPARc-signed contrast nanoparticles showing a high signal in thyroid tumour and showing only a background signal when the using nontargeted contrast nanoparticles (the region of interest were traced use blue).

Discussion
In recent years, numerous studies on the molecular biology and molecular mechanisms of tumors have confirmed that phosphatases play a key role in the regulation of tumorigenesis and development signaling; they are associated with abnormal cell proliferation and differentiation, and can be used as antitumor targets.PAX8-PPARc, a member of the PTP family, has been shown to be widely expressed in mammalian tissues and can regulate several cellular processes, including cell growth, cell differentiation, and the mitotic cell cycle, and abnormal PAX8-PPARc expression plays a key role in tumorigenesis, promoting the malignant biological process of several solid tumors, including breast, ovarian, lung, and liver cancer [10]. However, the role of PAX8-PPARc in the malignant evolution of thyroid tumors has not been reported yet. erefore, it is significant to investigate the role of PAX8-PPARc in thyroid tumors and its mechanism for thyroid cancer. In the present study, we showed for the first time that the PAX8-PPARc protein tyrosine phosphatase expression was significantly higher in thyroid cancer cell lines and thyroid cancer tissues relative to the normal thyroid cell lines and normal and paraneoplastic thyroid tissues, and this high expression of PAX8-PPARc may be involved in the development of thyroid cancer and is closely associated with patient prognosis, TNM staging, as well as lymph node metastasis (Table 1). e expression level of PAX8-PPARc was not significantly correlated with patient age and gender (P > 0.05).
is suggests that high PAX8-PPARc expression may be involved in the initiation In this study, we explored the relationship between the PAX8-PPARc expression changes and malignant biological behavior of thyroid tumors from in vivo and ex vivo experiments, respectively, and the results showed that PAX8-PPARc promoted the proliferation and antiapoptotic ability of thyroid cancer cells, and PAX8-PPARc expression inhibition affected the tumorigenic ability of nude mice in vivo. ese results suggest that PAX8-PPARc could serve as a marker to predict the malignant biological behavior of thyroid cancer and potentially represent a novel target as thyroid cancer treatment. is therapy may promote tumor cell apoptosis, thereby inhibiting cancer cell growth and metastasis, and it may delay tumor recurrence and metastasis, and improve the overall survival of thyroid cancer patients. [11,12].
With the continuous development of medical technology, coupling microbubbles with specific antibodies or drugs for the purpose of targeted contrast, drug delivery, and gene delivery has provided new ideas for the diagnosis of malignant tumors. Some studies have shown that targeted nanomicrobubble contrast agents have good specificity, and some scholars have applied the contrast agent specifically targeting VEGFR2/KDR in clinics, and this targeted contrast agent can significantly improve the signal intensity of lesions in patients with breast and ovarian cancer, which improves the efficiency of clinical diagnosis and provides a new starting point for the clinical translation of targeted contrast agents [13][14][15]. us, molecular imaging based on targeted nanomicrobubbles brings a new hope for early noninvasive diagnosis of tumors and has become a hotspot for research in the biomedical neighborhood in recent years [16].
In recent years, new methods based on mutations and other molecular markers have been partially applied in the differential diagnosis of thyroid tumors (e.g., BRAF, TP53, CTNNB1, and other genes), and these molecular markers have significantly improved the diagnostic accuracy of cytology and have had a profound impact on the classification and management of patients with thyroid tumors. However, the use of nanotechnology-based combined with specific antibody-targeted visualization for noninvasive imaging diagnosis of thyroid cancer has not been reported yet. Since our previous experiments confirmed that PAX8-PPARc was specifically expressed in solid tumor tissues of thyroid cancer and had an impact on its malignant biological behavior both in vivo and ex vivo, this would potentially provide a new molecular tumor marker for thyroid cancer and provide the necessary experimental basis for the next quantitative assessment of PAX8-PPARc aggregation in thyroid cancer in vivo by molecular imaging methods. erefore, we envisioned that PAX8-PPARc coupled with an ultrasound nanobubble contrast agent is needed to find a breakthrough point for early noninvasive diagnosis of thyroid cancer. In this study, we used PLGA nanospheres as a carrier and wrapped PFP inside to try to couple the PAX8-PPARc antibody on nanobubbles, which is an FDA-approved carrier for in vivo delivery of drugs and microRNAs with unique advantages such as excellent biocompatibility, but its targeting is a difficult problem to be solved. In this experiment, PLGA was conjugated with the PAX8-PPARc antibody by the carbodiimide method, and the surface of the prepared PLGA nanoparticles contained a large number of carboxyl groups. EDC/NHS was used as the coupling  activator to activate the carboxyl groups of the nanoparticles. en, it was combined with the amino group of streptavidin to form a stable covalent bond connection. In recent years, perfluorocarbons have been heavily used in various studies, and liquid fluorocarbons have a unique advantage, i.e., acoustic effect; however, the accumulation effect of liquid fluorocarbon emulsifiers in vivo alone to enhance ultrasound development is poor. In this study, perfluoropentane (PFP) with a boiling point of 29°C was used and wrapped in PLGA with a temperature threshold for the occurrence of liquid-gas phase transition which nearly doubled compared to the actual boiling point of PFP, and the targeted nanoparticles started to undergo phase transition when the temperature rose to 40-50°C. In the in vitro experiments, after the selected LIFU irradiation, the ultrasound image shows a significant acoustic enhancement effect on the nanoparticles of the PFP group, indicating that ultrasound can effectively induce the liquid fluorocarbon phase transition. e heating plate causes the liquid-gas phase change of nanoparticles only by temperature change regulation, while ultrasound can produce a cavitation effect and a mechanical effect in addition to the thermal effect, and these effects combined on PFP-targeted nanoparticles can lead to a greater possibility of phase change. In summary, the PFPtargeted nanoparticles prepared in this study can undergo liquid-gas phase transition in a short time under the excitation of LIFU, which provides a basis for the next in vivo experimental study.
PAX8-PPARc tyrosine phosphatase is encoded by the PTPN11 gene and regulates apoptosis, proliferation, and growth of hematopoietic stem cells in several signaling pathways. It has been shown that PAX8-PPARc overexpression may increase the risk of tumor metastasis in different types of cancers, including hepatocellular carcinoma, breast cancer, and colon cancer. Our previous findings confirmed that the degree of PAX8-PPARc expression in thyroid cancer tissues was positively correlated with tumor differentiation and progression, and that high PAX8-PPARc expression in thyroid cancer tissues also increased thyroid cancer metastasis.  yroid cancer is currently diagnosed clinically based on 2D ultrasound, and the gold standard for preoperative diagnosis mainly relies on fine needle aspiration cytology, but this is an invasive test. At present, ultrasonography disease is also used in the differential diagnosis of thyroid cancer, but its specificity and sensitivity are not high, which poses a problem for early diagnosis of thyroid cancer. Clinically used ultrasound contrast agents use lipid-encapsulated gas to enhance the ultrasound contrast by increasing the principle of backscatter, and some studies have reported that targeted ultrasound contrast agents have been used in the differential diagnosis of early pancreatic cancer, breast cancer, and ovarian cancer to improve the accuracy of ultrasound diagnosis. However, their micrometer diameter can only be used as a blood pool visualization within the blood vessels. Since PAX8-PPARc was confirmed to be specifically highly expressed in thyroid cancer tumor tissues in our previous study, and had low or no expression in normal thyroid tissues, this suggests that PAX8-PPARc may be a potential marker to identify thyroid tumors and may have some diagnostic specificity in ultrasound molecular imaging to increase the diagnostic accuracy.   In this study, PAX8-PPARc-targeted contrast agents were successfully prepared for in vitro and in vivo experiments to test the target binding specificity of targeted nanocontrast agents. e results of cellular assays showed that the targeted nanoparticles group (NPs-PAX8-PPARc) had a higher affinity for thyroid cancer cells compared to the nontargeted group (NPs-Control). In vivo target-seeking experiments in nude mice showed that the tumor ultrasound signal was significantly enhanced in nude mice injected with targeted and nontargeted nanoultrasound contrast agents via the tail vein immediately after irradiation with LIFU, while the tumor ultrasound signal enhancement was not significant in the nontargeted group. ese results suggest that PAX8-PPARc has the potential to be used as a specific Bax Caspase-3 Bcl-2 β-actin Figure 11: After treatment with NPs in SW579 cells, the expression of PAX8-PPARc-NPs, and DOX, the BAX and caspase-3 was significantly increased while the Bcl-2 expression was lower than that in any other group.  marker of the targeted contrast agent for real-time, noninvasive, and early diagnosis of thyroid tumors, and may provide a new theoretical basis and drug target for clinical targeted therapy of thyroid cancer, which has not been reported in the literature at home and abroad, and therefore will promote the further development of thyroid cancer diagnosis and treatment with high basic science and a high practical application value.
Data Availability e datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.

Conflicts of Interest
e authors declare that that have no conflicts of interest.