Monitoring Mycoplasma pneumoniae-Specific Antibody, C-Reactive Protein, and Procalcitonin Levels in Children with Mycoplasma Pneumonia Is Important

The goal of this study was to see how important it is to monitor Mycoplasma pneumoniae-speci ﬁ c antibody IgM, C-reactive protein, and procalcitonin levels in the blood of kids with Mycoplasma pneumoniae pneumonia as a reference for clinical diagnosis and treatment. The study group consisted of 96 children who had mycoplasma pneumonia in our hospital between May 2020 and May 2021, and the control group consisted of 96 healthy children who had a routine physical examination in our hospital at the same time. C-reactive protein and procalcitonin were measured and compared. The application value of single detection and combined detection of Mycoplasma pneumoniae-speci ﬁ c antibody IgM, C-reactive protein, and procalcitonin in the diagnosis of Mycoplasma pneumoniae pneumonia was evaluated based on clinical diagnosis results. The detection values of C-reactive protein and procalcitonin in the study group were higher than those in the recovery period and the control group, P < 0 : 05 ; the detection values of C-reactive protein and procalcitonin in the study group were higher than those in the control group, P < 0 : 05 . The combination detection of Mycoplasma pneumoniae-speci ﬁ c antibody IgM, C-reactive protein, and procalcitonin had a greater diagnostic accuracy than single detection ( P < 0 : 05 ). The sensitivity was higher than C-reactive protein and procalcitonin ( P < 0 : 05 ); the speci ﬁ city and positive predictive value were higher than Mycoplasma pneumoniae-speci ﬁ c antibody IgM ( P < 0 : 05 ); and the negative predictive value was higher than procalcitonin ( P < 0 : 05 ). The clinical value of combining the detection of Mycoplasma pneumoniae-speci ﬁ c antibody IgM, C-reactive protein, and procalcitonin in the diagnosis of Mycoplasma pneumoniae pneumonia in children is higher than that of single item detection, and it can provide a reliable clinical reference, as well as aid in evaluating the recovery e ﬀ ect of children, and it is worthy of application.


Introduction
In pediatrics, mycoplasma pneumonia is a frequent respiratory disease that affects school-aged and preschool children. Mycoplasma pneumonia is greatly affected by seasonal changes, children's physical resistance, and other factors. Mycoplasma pneumonia is characterized by rapid onset, long treatment cycle, and the need for continuous medication. In addition, the emotional instability caused by cough and fever in sick children also increases the difficulty of treatment [1]. Infection with Mycoplasma pneumoniae is the main cause of the disease. Because of their young respiratory systems, poor respiratory function, and inadequate immunity, children are more likely to contract the disease [2]. The disease's symptoms appear gradually. Weariness, faintness, and sluggishness are common in mild patients. An MP infection might be hazardous in some situations. If you have asthma, MP could make your symptoms worse. MP can possibly develop into a more serious pneumonia. MP complications include respiratory failure, lung abscess, acute respiratory distress syndrome, lung consolidation, and bronchiolitis obliterans. Symptoms such as dyspnea, headache, fever, and cough are common among severe patients. During auscultating lung sounds, tubular breath sounds and wet rales are common clinical symptoms.
Untimely or ineffective treatment can worsen the condition, harm small airway and respiratory function, and even cause encephalitis, myocarditis, and other complications [3]. Mycoplasma pneumonia has obvious symptoms such as persistent high fever and aggravated cough. If it is not treated in time, it may form lobar pneumonia. At the same time, mycoplasma infection can also cause myocardial damage and liver function damage and seriously reduce the diet and mental state of children. Some children will cause central nervous system infection, and the infection of mycoplasma encephalitis will affect life safety [4]. As a result, in clinical treatment, it is critical to diagnose and treat mycoplasma pneumonia as soon as feasible. The clinical value of combining the detection of Mycoplasma pneumoniaespecific antibody IgM, C-reactive protein, and procalcitonin in the diagnosis of Mycoplasma pneumoniae pneumonia in children is higher than single item detection, and it can provide a reliable clinical reference, as well as aid in evaluating the recovery effect of children, and it is worthy of application.
In recent years, projects based on serum targets such as Mycoplasma pneumoniae-specific antibody, C-reactive protein, and procalcitonin, among others, have been developed to provide a reliable diagnostic basis for clinics, which is critical for improving Mycoplasma pneumoniae clinical diagnostic efficiency [5][6][7]. The variance of Mycoplasma pneumoniae specific antibody, C-reactive protein, and procalcitonin in the blood of 96 children with mycoplasma pneumonia and 96 healthy children was compared by pediatricians at our hospital. Monitoring the levels of Mycoplasma pneumoniae-specific antibody, C-reactive protein, and procalcitonin in children with Mycoplasma pneumoniae pneumonia is helpful for the diagnosis of Mycoplasma pneumoniae pneumonia and the evaluation of children's condition. The main structure and ideas of this study are shown in Figure 1. In addition, 96 healthy children who underwent routine physical examination in pediatrics of our hospital meanwhile were classified into the control group. There were 50 girls and 46 boys; the age ranged from 1 to 14 (6:16 ± 1:00) years. The conditions of the control group are as follows: P values are the same in the comparison of gender and age between the two groups that is >0.05.

2.2.
Methods. The children in the study group collected 2~3ml of upper limb venous blood on an empty stomach at the beginning of admission (acute stage) and recovery stage, and the children in the control group collected 2~3 ml of upper limb venous blood on an empty stomach at one time. The blood samples were centrifuged, placed in a vacuum tube, posited for 30 min, centrifuged at 3500 r/ min for 10 min, and the supernatant was extracted for later testing. In the detection of Mycoplasma pneumoniae-specific antibody IgM, indirect enzyme-linked immunosorbent assay is used for detection. The kit is provided by the Shanghai Hushang Biotechnology Co., Ltd. It is regarded positive if the detection well's color rendering is better than the control well's. Immune scattering turbidimetry is used to identify C-reactive protein, which is detected with the Nephstar Plus device. The kit is provided by Siemens Medical Diagnostic Products (Shanghai) Co., Ltd. The result of detection is considered positive if the detection value is >8 mg/l [9]. In procalcitonin detection, chemiluminescence method is used for detection with Roche Cobas e601 electrochemiluminescence immunoanalyzer, and the kit is provided by Merier Diagnostic Products (Shanghai) Co., Ltd; the test value >0.5 ng/ml is considered positive [10].

Observation Index
(1) Compare the detection results of specific antibody IgM, C-reactive protein, and procalcitonin of Mycoplasma pneumoniae between the two groups (2) Based on the clinical diagnosis results, the application value of single and joint detection of Mycoplasma pneumoniae-specific antibody IgM, Creactive protein, and procalcitonin in the diagnosis of Mycoplasma pneumoniae pneumonia were evaluated, and the accuracy, sensitivity, specificity, positive predictive value, and negative predictive value were calculated 2.4. Statistical Analysis. Statistical data analysis was performed with SPSS 22.0; normally enumeration data are expressed as % and inspected by chi-square test, and the data are expressed in ð±sÞ and performed by t-test. A P value of <0.05 was considered statistically significant.

Comparison of Detection Results of Specific Antibody
IgM, C-Reactive Protein, and Procalcitonin of Mycoplasma pneumoniae between the Two Groups. At the time of admission, the study group had higher rates of positive Mycoplasma pneumoniae-specific antibodies IgM, C-reactive protein, and procalcitonin, as well as higher detection values of C-reactive protein and procalcitonin, than the recovery group and the control group (P < 0:05). The detection values of C-reactive protein and procalcitonin in the study group were higher than those in the control group (P < 0:05). They are presented in Table 1.

Comparison of Application Value of Single and Combined Detection of Mycoplasma pneumoniae-Specific
Antibody IgM, C-Reactive Protein, and Procalcitonin in the Diagnosis of Mycoplasma pneumoniae Pneumonia. The diagnostic accuracy of combined detection of Mycoplasma pneumoniae-specific antibody IgM, C-reactive protein, and procalcitonin was higher than that of single detection (P < 0:05); the sensitivity was higher than that of Creactive protein and procalcitonin (P < 0:05); the specificity   and positive predictive value were higher than those of Mycoplasma pneumoniae-specific antibody IgM (P < 0:05); the negative predictive value was higher than that of procalcitonin (P < 0:05). They are presented in Tables 2 and 3.

Discussion
The gold standard for clinical diagnosis of Mycoplasma pneumoniae is isolating culture. However, because Mycoplasma pneumoniae grows slowly and takes a long time to isolate, it cannot produce results rapidly, which is inconvenient for early disease therapy, resulting in its limited clinical application [11]. With the deepening of relevant researches, it is considered that Mycoplasma pneumoniaespecific antibody, C-reactive protein, and procalcitonin can play an important role in the diagnosis and efficacy evaluation of Mycoplasma pneumoniae pneumonia in children and are important supplementary indicators for the isolation and culture of Mycoplasma pneumoniae [12][13][14].
After infection with Mycoplasma pneumoniae in humans, specific IgM, IgG, and IgA antibodies will be produced in them, of which IgM antibodies appear first. Under normal circumstances, they only exist in serum 60~90 days after infection, so they have become an effective marker of recent infection [15,16]. Relevant studies have also found that the incubation period of Mycoplasma pneumoniae infection is 14~21 days, and the IgM antibody in children's serum has reached a very high level when they have symptoms [17]. C-reactive protein (CRP) is a liver-produced acute phase reactive protein that can bind with choline phosphate in bacterial cell walls to activate complement activity and regulate cell phagocytosis [18]. When the body is stressed by an inflammatory reaction, the liver produces a huge amount of C-reactive protein, resulting in a considerable increase in its expression level [19]. The sensitivity of Creactive protein is high, and its detection value is not affected by the patient's age, gender, body temperature, and other factors. Procalcitonin, a precursor of calcitonin, is a protein secreted by thyroid C cells. The body generally cannot detect PCT under healthy conditions. When the body has inflammation or infection, it will release a large number of inflammatory cytokines. The combination of inflammatory cytokines and bacterial toxins will produce a large number of procalcitonin, resulting in a significant  5 Computational and Mathematical Methods in Medicine increase in its detection value. Studies have found that the higher the detection value of procalcitonin, the more serious the infection [20]. At present, it is generally believed that when antibiotics are used in clinical treatment of mycoplasma pneumonia in children, procalcitonin level can be used as a reference index to provide medication guidance. Once the serum procalcitonin of children reaches 0.5 ng/ml, it should be used in time, so as to shorten the antibacterial course and improve the overall curative effect [21]. Through the use of appropriate dose of budesonide atomization therapy combined with azithromycin administration, it has obvious effect on children with mycoplasma pneumonia, which can quickly alleviate children's cough and improve the clinical efficacy [22]. The use of montelukast sodium chewing combined with azithromycin administration has a significant effect on children with mycoplasma pneumonia, which can effectively improve patients' lung function and alleviate the symptoms of mycoplasma pneumonia [23].
The findings of this study revealed that the positive rates of Mycoplasma pneumoniae-specific antibody IgM, C-reactive protein, and procalcitonin, as well as the detection values of C-reactive protein and procalcitonin, were higher in the study group at the start of hospitalization than in the recovery period and control group, implying the high expression of Mycoplasma pneumoniae-specific antibody IgM, C-reactive protein, and procalcitonin in children with myco, while the detection values of the three indexes of children have decreased significantly after treatments, and the positive detection rate has no significant difference compared with the control group. In case of that, it is considered that the specific antibodies IgM, Creactive protein, and procalcitonin of Mycoplasma pneumoniae can be used as reference indexes for clinical diagnosis and curative effect evaluation of children with Mycoplasma pneumoniae pneumonia. The results of diagnostic analysis showed that compared with the detection of each single index, the accuracy of the three indexes after the joint detection in the diagnosis of mycoplasma pneumonia was significantly higher, and the sensitivity, specificity, positive predictive value, and negative predictive value were improved in varying degrees, indicating that the joint detection can further improve the accuracy of diagnostic results and provide more accurate reference basis for clinical practice.

Conclusion
As a consequence, combining the detection of Mycoplasma pneumoniae-specific antibody, C-reactive protein, and procalcitonin in the diagnosis of children with Mycoplasma pneumoniae pneumonia has a higher clinical application value than single item detection, which can provide a reliable reference basis for clinical practice and aid in evaluating the recovery effect of children, and is worthy of application. The emotional instability of children with mycoplasma infection, persistent fever, and cough affect the treatment and diet of children. We can consider increasing nutritional supplement and related psychological support to speed up the recovery of children.

Data Availability
The data used to support the findings of this study are included within the article.

Conflicts of Interest
The author declares that they have no conflicts of interest.