Study of Endogenous Paramagnetic Centers in Biological Systems from Different Areas

Institute of Radiation Problems, Azerbaijan National Academy of Sciences, Baku, Azerbaijan Department of Biophysics and Biochemistry, Baku State University, Baku, Azerbaijan Joint Ukraine-Azerbaijan International Research and Education Center of Nanobiotechnology and Functional Nanosystems, Drohobych, Ukraine & Baku, Azerbaijan Department of Biochemistry, Azerbaijan Medical University, Baku, Azerbaijan Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine /e John Paul II Catholic University of Lublin, Lublin, Poland Department of Biophysics, Moscow State University, Moscow, Russia Medical Laboratory Techniques Vocational Higher School of Healthcare Studies, Mardin Artuklu University, Mardin 47200, Turkey Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran Drohobych Ivan Franko State Pedagogical University, Drohobych, Ukraine


Introduction
Magnetic nanoparticles are widely found in many biological objects. Long before the synthesis of the first magnetic nanoparticles, they were discovered in natural biological complexes. It has been established that magnetic nanoparticles play a role in the metabolism and activity of living organisms [1][2][3][4][5]. e most common magnetic nanoparticles in living organisms are magnetite (Fe3O4) and maghemite (c-Fe2O3). Magnetic nanoparticles can be found in bacteria and cells of higher living organisms [6][7][8][9]. It is believed that anisotropic magnetic nanoparticles in the cell can interact with the earth's magnetic field and transmit relevant information to other receptors in the body [10][11][12][13]. Since the application of magnetic nanoparticles in various fields is very important, it is very topical to carry out their synthesis by biological methods, as well as to clarify the role of biological processes in the synthesis of these nanoparticles. e broad EPR signals characterizing iron oxide magnetic nanoparticles in the leaves and seeds of various plants (autumn olive (Elaeagnus angustifolia L.), Syrian bean-caper (Zygophyllum fabago L.), camel-thorn (Alhagi Pseudalhagi), highland rush (Juncus trifidus L.), etc.) collected from radioactively contaminated areas of the Absheron Peninsula studied by us by EPR spectroscopy. e results of the experiments showed that the broad EPR signal, which characterizes the magnetic nanoparticles, is characteristic of leaf samples. Also, in the seeds, only EPR signals characterizing free radicals and manganese ions were observed. Radioactive contamination (130-200 μR/h) led to an increase in the amplitude of EPR signals in both cases [14].
In this work, the leaves of plants (Eldar pine (Pinus eldarica M.), fig (Ficus carica L.), and olive (Olea europaea L.)) collected from different areas according to their ecological status were studied comparatively by Electron Paramagnetic Resonance (EPR) spectroscopy. With help of this method, the generation of iron oxide magnetic nanoparticles in biological systems as a result of the process of biomineralization under the influence of stress factors has been discovered. It has been found that stress factors have a stimulating effect on this phenomenon.

Materials and Methods
From two different areas of the Absheron Peninsula in terms of the ecological situation (Ahmadli settlement and Babek Avenue) were collected leaves of Eldar pine (Pinus eldarica M.), fig (Ficus carica L.), and olive (Olea europaea L.)) for research. After the plants were dried at room temperature, their EPR spectra were recorded. A new approach based on the effect of biomineralization has been applied to these plants as a response to stress reduction and prevention. e spectra of plant leaves collected from different areas according to the degree of environmental pollution were studied with the help of an X-band EPR Spectrometer (BRUKER EMX, Germany, resolution: 1024 points, sweep width: 55000T).

Results and Discussion
In experiments with Eldar pine (Pinus eldarica M.) leaves collected from different areas (Ahmadli settlement and Babek Avenue) according to the degree of environmental pollution, the generation of magnetic nanoparticles of biogenic origin was studied with the help of an X-band EPR spectrometer (BRUKER, Germany) ( Figure 1). Spectra of conifers (leaves) collected from the Eldar pine (Pinus eldarica M.) tree were recorded at room temperature. Two types of signals were detected in the recorded spectra, a free-radical signal (g � 2.0023) and a broad EPR signal (g � 2.32; ΔH � 32 mT), characterizing nanophase iron oxide magnetic particles. e behavior of the signal parameters     suggests that the samples collected from Babek Avenue contain more biogenic magnetic nanoparticles, and therefore, it is possible to assume that this area is more exposed to environmental pollution (Figure 1). Angular variations were also studied in the samples. To determine the variation of the angle in the resonator, the glass ampoule in which the samples were placed was examined at different angles (90°, 180°, 270°, and 360°) (Figure 2). As a result, we observed a slight change in the shape and location of the EPR signal. In this case, the samples were normalized on unit mass.
Such behavior of signals has been found for magnetite (Fe3O4), maghemite (c-Fe2O3), and superparamagnetic nanoparticles. is indicates that the paramagnetic centers responsible for the EPR signal have a complex structure.
At the same time, paramagnetic centers in the leaves of Olea Europea L. and Ficus carica L. trees have also been studied (Figures 3-5).
Based on the behavior of the parameters of the recorded signals, the EPR spectra of Eldar pine (Babek ave.

Conclusions
EPR studies with the leaves of three different tree plants in Absheron (Pinus eldarica M., Olea Europea L., and Ficus carica L.) have once again shown that, under stress, they generate signals characterizing iron oxide magnetic nanoparticles (g � 2.32; ΔH � 40 mT). is result is of great importance in biomedical research. e recorded EPR spectra showed that more iron oxide magnetic nanoparticles are generated in olive leaves. e study of the angular variations of the signals of olive leaves showed that this signal has a magnetic anisotropy ( Figure 6). It was observed that the EPR signal shifted to the left when the ampoule containing the samples in the resonator of the spectrometer was rotated 90°, 180°, 270°, and 360°. Such behavior of signals has been found for magnetite (Fe 3 O 4 ), maghemite (c-Fe 2 O 3 ), and superparamagnetic nanoparticles. is indicates that the paramagnetic centers responsible for the EPR signal have a complicated structure [14,15].
It was found that the process of biomineralization plays a role in the formation of biogenically iron oxide magnetic nanoparticles in plants, in the generation of magnetite crystals in biological tissues, and stress factors have a stimulating effect on this effect. e effect of the influence of radiation factors discovered by us can be used in the biosynthesis of functional iron oxide nanoparticles applied in various fields. Detection of iron oxide nanoparticles by EPR signals can be used in biomedical research. e intensity of EPR signals characterizing magnetic nanoparticles can be an indicator of the impact of stress factors (environmental pollution, radiation, UV radiation, etc.). e parameters of EPR signals give a reason to comment on the environmental situation in these areas. Studies by EPR spectroscopy have shown that this method is a very promising method for detecting the formation of magnetic nanoparticles of iron oxide in biological systems and can provide new information in the determination of pollution levels of natural systems, environmental assessment, and biomonitoring. e results of EPR research are informative for radioecological and radiobiological research.

Data Availability
e data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest
e authors declare no conflicts of interest.