Development of Electron Paramagnetic Resonance Magnet System for In Vivo Tooth Dosimetry

As part of a homebuilt continuous wave electron paramagnetic resonance (EPR) spectrometer operating at 1.2 GHz, a magnet system for in vivo tooth dosimetry was developed. The magnet was designed by adopting NdFeB permanent magnet (PM) for the main magnetic ﬁeld generation. For each pole of the magnet, 32 cylindrical PMs were arranged in 2 axially aligned ring arrays. The pole gap was 18cm, which was wide enough for a human head breadth. The measured magnetic ﬁeld was compared with the magnetic ﬁeld distribution calculated in a ﬁnite element method (FEM) simulation. EPR spectra of intact human teeth irradiated 5 and 30Gy were measured for the performance test with the developed magnet system and spectrometer. The measured mean magnetic ﬂux density was estimated to be 44.45mTwith homogeneity of 1,600ppm in a 2 cm diameter of the spherical volume of the XY plane, which was comparable to the FEM simulation results. The sweep coeﬃcient of the magnetic ﬁeld sweep coil was 0.35 mTper Ampere in both the measurement and FEM simulation. With ± 9A current, the sweep range was 5.7 mT, which was suﬃciently wide to measure the tooth radiation-induced signal (RIS) and reference material. The peak-to-peak amplitude of the measured modulation ﬁeld was 0.38mT at the center of the magnet. With the developed magnet fully integrated into an EPR system, the EPR spectra of 5 and 30 Gy irradiated teeth were successfully acquired. The developed magnet system showed suﬃciently acceptable performance in terms of magnetic ﬂux density and homogeneity. The EPR spectrum of tooth RIS could be measured ex vivo . The RIS of 5 and 30 Gy irradiated teeth was clearly distinguishable from intact human teeth.


Introduction
e triage of a large population is a critical social demand when a nuclear accident occurs, such as Chernobyl disaster in 1986 and Fukushima Daiichi nuclear accident in 2011. While the number of potential patients is large, the individual radiation damage is widely distributed from a slight to a life-threatening dose. Depending on the degree of significance, proper management and treatment of injuries are urgently required [1].
is is why triage is critically demanded to assess the radiation dose. In addition, a quick assessment of radiation dose for individuals is required to be performed on-site, but technologies for such are limited. e current gold standard for dose assessment in exposed individuals is a dicentric scoring analysis [2]. However, dicentric analysis is a laborious and time-consuming method that requires 72-96 h for lymphocyte culture and manual scoring by an expert. Even with advanced improvements, such as automated dicentric analysis, it takes 48 h only for cell culturing [3].
In vivo electron paramagnetic resonance (EPR) tooth dosimetry is a currently available technique for radiation dose assessment for human subjects noninvasively. EPR has been employed in radiation dosimetry by quantifying the amount of radicals generated by ionizing radiation [4]. Ionizing radiation generates stable CO 2 − radicals in calcified human tissues, such as tooth enamel and bone. In vivo EPR tooth dosimetry is useful, especially in radiological accidents, where most potential victims do not possess appropriate dosimeters for its expeditiousness [5]. It can rapidly assess an exposure dose in 10 min, including 5 min of measurement and 5 min of assessment. Such advantages have been exploited to estimate victims' exposure retrospectively in radiation accidents [6][7][8].
e in vivo method investigates intact human teeth noninvasively without preprocessing to use noninvasiveness, on-site response, and expeditiousness during dose estimation. In the conventional method, a microwave frequency of 9 GHz or higher is used, which is easily absorbed into aqueous material in in vivo studies. us, most in vivo studies chose low frequencies around 1 GHz to avoid the interference of water [5,9]. Some scholars tried using X-band frequency for in vivo tooth dosimetry together with a modified X-band resonator [10,11]. Owing to the lack of commercially available spectrometers for human studies, specific devices have to be developed for in vivo studies aiming at human applications. Hirata et al. [12] developed an electronically tunable resonator for in vivo EPR measurement. Guo et al. [10,11] also developed a resonator to measure only in vivo tooth dosimetry using X-band.
In addition, the in vivo method is relatively easy for unskilled workers to assess the radiation dose of an exposed person, making in vivo EPR tooth dosimetry suitable for an on-site patient triage tool. To deploy EPR-based dosimetry instruments to places close to a disaster area or the shelter of evacuees, EPR instruments should be mobile and easily operated. However, the magnet of an EPR spectrometer and its power supply are generally heavy. is is an obstacle to transfer the EPR spectrometer from a laboratory to a field near a disaster area.
It is known that using permanent magnet (PM) arrays reduces the weight of the magnet at a low cost [13]. A carmounted magnetic resonance imaging system for on-site diagnosis has been proposed [14]. e magnet was 200 kg in weight, which was deployable using a car. Swartz et al. [15] developed a deployable EPR spectrometer for in vivo tooth dosimetry, including the magnet. e magnet weighed 30 kg [16]. Numerous studies on in vivo EPR dosimetry were recently reviewed. Sato-Akaba et al. [17] used small neodymium magnet arrays to form a homogeneous magnetic field for biological EPR imaging. e magnet combined with coils weighed 6 kg. Sirota et al. developed a magnet for pulsed EPR tooth dosimetry by adopting the methodology of ex situ nuclear magnetic resonance (NMR) [18]. ey also tried in vivo tooth dosimetry with another type of magnet under an 11.2 GHz frequency [19].
In this study, we develop a magnet system using PMs with deployable weight for in vivo EPR tooth dosimetry. e magnet system for in vivo EPR dosimetry is a key component to be developed by in-house users. Although there have been many studies to develop a magnet system for general purposes, including NMR and magnetic hyperthermia, the number of EPR studies describing the development of magnet systems for in vivo tooth dosimetry is limited [20][21][22]. e development described in this study is part of the entire development of an in vivo EPR spectrometer for tooth dosimetry. In this study, a magnet system focusing on in vivo EPR tooth dosimetry was developed using PMs and copper coils. First, the design and fabrication of the magnet system are described. en, the performance of the magnet system is evaluated in terms of magnetic flux density and uniformity. Finally, ex vivo EPR spectra were measured to verify the magnet system's performance.

Design Concept and Required Specifications.
As mentioned above, we fabricated an EPR magnet with deployable weight for in vivo tooth dosimetry in this study. erefore, the required specifications are determined from the viewpoint of weight (deployability), pole gap width, main magnetic flux density, magnetic field homogeneity, sweep field width, and amplitude of the modulation field.
For the magnet to be deployable, at least by a car, it should be light enough to be loaded onto a vehicle by one person. Based on the study by Williams et al. [16], the weight should be equal to or lower than 30 kg. e pole gap of the magnet was determined considering the subject's head size. Since the subject's head is located between the pole gap to measure the tooth in vivo, a sufficiently wide space should be taken between the two poles of the magnet. is would be 18 cm due to a statistical reason given later in the part where the EPR magnet's design was described.
Meanwhile, the main magnetic flux density, B 0 , and homogeneity required for tooth dosimetry should be secured. e B 0 is determined following the microwave frequency used for operation. A high frequency tends to be absorbed by tissues around the measured tooth. As such, frequencies of approximately 1.2 GHz have been adopted as the detection frequencies in several preclinical and clinical systems, compromising sensitivity and detection depth [9]. e required B 0 was calculated to be 42.9 mT. e B 0 field needs to be sufficiently homogeneous over the sample volume [23]. e least required homogeneity of B 0 is determined by the variation of B 0 over the sample volume and the linewidth of the investigated sample. As a rule of thumb, the variation in the magnetic field strength over the sample should be less than 10% of the linewidth of the sample signal [23]. For tooth dosimetry, the linewidth of the radiationinduced signal (RIS) of a tooth is known to be 0.26 mT [9,24,25], so the required B 0 variation is 0.026 mT. e scannable range of the magnetic field should include the spectrum of the reference material, 4-oxo-2,2,6,6-tetramethylpiperidine-d 16 -1-15 N-1-oxyl ( 15 N-perdeuterated tempone ( 15 N-PDT), CDN Isotopes, Quebec, Canada) and that of the tooth. e least sweep range required for this is approximately 3.5 mT. e amplitude of RIS of the tooth EPR spectrum is known to be maximized at 0.4 mT field modulation. In our design, a modulation field of 0.4 mT is planned to be applied to the tooth sample location. e modulation frequency should be more than 20 kHz, which is a limitation of audible frequency due to its in vivo application. Figure 1 shows a schematic design of the EPR magnet system for in vivo tooth dosimetry. e magnet system typically comprises PMs, magnetic field sweep coils, and magnetic field modulation coils. PMs are used to generate the Zeeman magnetic field of the L-band (1.2 GHz in this study). In continuous wave (CW) EPR, the spectrum is acquired by scanning magnetic fields around the main magnetic field (B 0 ). is spectrum is acquired in the presence of an alternating current (AC) magnetic field formed by the magnetic field modulation coils.

EPR Magnet Configuration.
B 0 is generally provided by electromagnets in commercial EPR spectrometers using relatively higher frequencies, such as X-or Q-bands. PMs are also available in applications of L-band or lower frequencies, which are broadly used for in vivo measurements. Adopting PMs for B 0 has an advantage over electromagnets by reducing the number of devices for electromagnet operation, such as a power supply and cooling system.
Each magnetic field sweep coil comprises two separate axially aligned identical circular coils operated with direct current (DC). By applying DC to the sweep coil, the main magnetic field varies in strength. Each magnetic field modulation coil also comprises two identical circular coils operated with AC.

EPR Magnet
Design. B 0 is static and equivalent to the Zeeman magnetic field of the subject material under investigation. Sintered Nd 2 Fe 14 B (NdFeB) was adopted for the PM material. NdFeB is one of the strongest commercially available PM materials. Cylindrical PMs with a 2.5 cm diameter and 6.2 cm length were used. A total of 32 NdFeB cylindrical magnets were used to make 2 ring arrays, which were axially aligned (blue in Figure 1). us, 16 PMs were aligned parallel in each ring. e magnetic flux density generated by the two PM ring arrays is measured at the center region of the two axially aligned rings, where a subject for EPR measurement is positioned.
Between the two axially aligned ring arrays, the space where a subject's head is located for in vivo tooth dosimetry should be considered. A homogeneous magnetic field region is formed around the center between the two ring arrays. For in vivo measurement, a homogeneous magnetic field should be formed where the upper incisors are located when a subject's head is put between the two poles of the magnet.
Some studies statistically estimated the human head size of ethnic groups [26][27][28]. e head breadth is the maximum horizontal width of the head above the ears and is used to determine the pole gap. From the Civilian American and European Surface Anthropometry Resource (CAESAR) database of North Americans, the maximum head size was estimated to be 17.2 cm in both genders of Caucasian, African, Asian, and Hispanic [28]. e pole gap of 18 cm would be enough to examine most people, although the top 5% of the male group was reported to have a head breadth of 18.2 cm in another study targeting Taiwanese. In this study, the actual gap width between two PM ring arrays was determined to be 19 cm together with a lamination plate of 0.5 cm thickness attached to the inner face of each pole.
e sweep coil has an inner and outer radius of 9.0 and 10.29 cm, respectively, with a width of 3.95 cm, which is placed on the surroundings outside PMs (brown in Figure 1). e EPR measurement for tooth dosimetry requires a sufficient magnetic sweep range to include spectra combined with signals of the reference materials and tooth RIS. In this study, 15 N-PDT was used as a reference material, of which the spectrum had two peaks sufficiently included within a 3.5 mT magnetic field sweep when using a 1.2 GHz frequency. To satisfy this requirement with a reasonably tolerable current, 100 turns were wound with a copper wire of 2.2 mm diameter on each side of the sweep coil. e gap between both sides of the coils was the same as that of PMs. e number of turns and diameter of the coils were determined by the guidance of the finite element analysis (FEM) simulation stated below. e magnetic field modulation coil operates at 21.2 kHz. e modulation coil has an inner and outer radius of 3.3 and 3.97 cm, respectively, with a width of 3.6 cm, which is placed inside the PM ring arrays (red in Figure 1). Eighty-two turns are wound with a copper wire of 1.6 mm diameter on each side. Owing to the characteristic of AC magnetic field inducing eddy current in adjacent conductive materials, the parts nearby were built with nonconductive materials, except for coils and PMs.

Analytical Calculation of Magnetic Flux Density of PMs.
At the design stage, B 0 was calculated to be 42.9 mT assuming ] � 1.2 GHz when g of the radiation-induced radical of the tooth was approximately 2.0. us, the grade of the NdFeB magnet was determined to adjust B 0 close to 42.9 mT.
To adjust the central magnetic field to the Zeeman magnetic field, the magnetic field generated by PMs was calculated. e central magnetic field was calculated as the sum of the magnetic fields of each PM. e remanent flux density, B r , of PM was determined as a nominal value of 1.31 T, which was close to the NdFeB grade of N42.
e details of this calculation are described in Appendix.  [29]. For electromagnetic simulation, the physics interface magnetic field (mf ) available from AC/DC module was adopted to compute the magnetic field. e geometry of the magnet system in FEM simulation was simplified to save calculation time. e geometry included PMs, magnetic field modulation coils, modulation coil reels, magnetic field sweep coils, and sweep coil reels. Although the sweep and modulation coil geometries could comprise a torus for each turn of the coil wire, the coils were simplified into tubes. Otherwise, the torus geometry would require significant computational time to calculate a large number of meshes composing tori. e coils were defined using the multiturn coil feature. Nonmagnetic components, including the casing and cover, were omitted. Materials applied for each component are listed in Table 1.

Magnetic Field Measurement.
A magnetic flux density of PMs was measured over a 2 cm diameter of a spherical volume (DSV) at the center of the magnet. According to a dental study, the dimension of the human upper incisor in the oral cavity is 8.73-9.3 mm in width and 10.4-11.2 mm in length [30]. us, a 2 cm DSV is wide enough to cover the two upper incisors in a subject's oral cavity. For conservative assessment, the DSV was larger than the volume occupied by the two upper incisors by a wide margin.
e homogeneity was calculated from the following equation: A gaussmeter (DTM-151 Digital Teslameter, Group 3 Technology, Auckland, New Zealand), to which a hall probe (MPT-141 Hall Probe, Group 3 Technology, Auckland, New Zealand) was attached on a platform moving with a conveyer belt, was used to measure the magnetic field. Magnetic flux density from −7 to 7 cm on the X-axis and −5 cm to 5 cm on the Y-axis at the center of the magnet was measured. In addition to the volume data included in 2 cm DSV, the magnetic flux density on X-and Y-line profiles was measured for comparison with the FEM results.
e magnetic flux density of a modulation coil was measured using a search coil magnetometer along the X-and Y-axis around the magnet's geometric center. e search coil comprised ten turn copper coils with a radius of 3.82 mm. e region from −3.5 to 3.5 cm along each axis was measured at a 0.5 cm increment. e magnetometer was connected to an oscilloscope so that the peak-to-peak amplitude of the induced voltage was measured. e strength of the modulation field was evaluated by converting the voltage into the magnetic field.

EPR Spectrum
Acquisition. An EPR spectrum was acquired to verify the performance of the magnet system combined with an EPR system for in vivo tooth dosimetry that had been developed at Seoul National University. e magnet pole gap was adjusted to 18.4 cm to lower B 0 closer to the calculated value of 42.9 mT. e spectrometer system used to acquire EPR spectra is comprised of the developed magnet system, a spectrometer controller system, a microwave bridge, and a tunable resonator (Figure 2(a)). e spectrometer systems except for the developed magnet were tested using a magnet that was described in our previous paper [31].
e magnet system's sweep coils were operated with a bipolar power supply controlled by a controller system. e modulation coils were connected to an amplifier, to which a 21.2 kHz input signal was supplied from the controller system. e acquired data was transmitted to the receiver of the controller. e circuit connection of the modulation and sweep coils is shown in Figure 2(b). To operate the modulation coils (L M1 and L M2 in Figure 2(b)) at 21.2 kHz, an LC series  Figure 1: A schematic design of EPR magnet system for in vivo tooth dosimetry: the magnet system typically comprises PMs (blue), sweep (brown), and modulation coils (red). It should be noted that the direction of the main magnetic field is on the X-axis.
resonance circuit was used. e sweep coils (L S1 and L S2 in Figure 2(b)) were located close to the modulation coil so that AC was induced on it by the AC magnetic field. is induces not only unintended AC on the sweep coils but also the AC magnetic loss of the modulation field. To reduce this, an LC low-pass filter was connected to the sweep coils. e EPR spectrum was accumulated ten times for 3 s for each field sweep. Ten spectra were collected for each sample. e peak-to-peak amplitude of the first harmonic signal was estimated. e RIS of two intact human upper incisors was measured after 5 and 30 Gy X-ray irradiation for each. As a reference material, 15 N PDT was prepared in a thin Teflon tube after diluting to 0.1 mM.

Characteristics of Prototype Magnet
System. In this section, the actual characteristics of the described magnet system are described briefly. e total weight of the magnet was 22 kg. e weight is 27% lighter than that of the in vivo tooth dosimetry study by Williams et al. [16]. e pole gap width was 18 cm. e main magnetic field was measured to be 44.5 mT at 2 cm DSV of the magnet system's center. Its homogeneity was 0.07 mT in B 0 variation measured along the X-axis in the 2 cm DSV, but it was estimated to be useable, approximately satisfying the 0.026 mT requirement when a tooth is the subject of measurement. e sweep width was 5.7 mT in the current range of ±9 A, sufficiently requiring the aimed specification. e modulation field amplitude was 0.38 mT.

3.2.
e Magnet System Building. e designed magnet system was manufactured by Hanmi Techwin, Siheung, Republic of Korea. e developed magnet system is shown in Figure 3. e sweep and modulation coils were independently manufactured and assembled so that they are ) and sweep coils (L S1 and L S2 ). LC series resonant is used to operate the modulation coils with 21.2 kHz. In sweep coil circuitry, LC low-pass filter is connected to block the AC induced by the AC magnetic field from the modulation coil. independently exchangeable in case of malfunction. e sweep coils are located at the outermost location of the magnet system, whereas the PMs are invisible from the outside (Figure 3(a)). From the side view of the magnet system, some modulation coils and their wiring are seen (Figure 3(b)). e modulation coils are wound with reels made of monomer casting nylon, and their outer sides are exposed to air. All metallic parts, except PMs and coils, were made of brass or stainless steel, which are nonmagnetic. e pole gap has a minimum of 17.8 cm and is extendable up to 19 cm by adjusting 16 hexagon nuts and fixing the location of both sides of the magnet system.

Prototype Magnet System.
e magnetic flux density profiles along the X-and Y-axis are presented in Figure 4  e mean values and homogeneity of the magnetic field density profiles are summarized in Table 2. As described in Appendix, the analytical calculation was performed to confirm the magnetic flux density of PMs. e measured homogeneities were higher, especially in Figure 4(d), due to the inevitable vibration of a hall sensor during measurements.
After smoothing the profiles with the moving average method, the homogeneity of the Y-axis and XY plane became 337 and 1600 ppm, respectively.
With the smoothed measured data in the Y-axis, the B 0 variation was 0.014 mT, which satisfied the requirement of less than 0.026 mT. However, the raw B 0 variation in the XY plane was 0.07 mT and not satisfactory to the homogeneity requirement. As mentioned earlier, the actual B 0 variation was smaller than this; the homogeneity was calculated conservatively within a volume larger than the tooth.
e B 0 variation of the XY plane is 0.028 mT if calculated in the region of 1 cm on the X-axis and of 0.2 cm on the Y-axis around the geometric center. is is nearly satisfactory to the 10% linewidth requirement of RIS. is region could be applied only when one incisor is located at the center.
e magnet system was designed to adjust its pole gap between 18 and 19 cm in case of a situation requiring a change of B 0 strength, which is often caused by a difference (or error) in the remanent flux density of PM from the nominal value. e variation of magnetic flux density versus pole gap distance was calculated in the 2 cm DSV via FEM simulation ( Table 3). As the pole gap varied from 18 to 19 cm, B 0 decreased from 43.5 to 41.2 mT. To tune at the 1.2 GHz system frequency of the entire EPR spectrometer, the pole gap was determined to be 18.4 cm.

Sweep Coil.
e magnetic flux density of the sweep coils was measured in the XY plane.
e measurement values were compared with FEM simulation values (Figure 4). e mean value and homogeneity with a current in the sweep coil are listed in Table 4. After smoothing the measured Y-axis line profile, the homogeneity became 999 and 611 ppm for −4 and 4 A, respectively. e mean value and homogeneity on the XY plane were evaluated as 45.85 mT and 1381 ppm for 4 A and 43.30 mT and 2350 ppm for −4 A after smoothing the measured values.
e sweep efficiency was measured as 0.35 mT per Ampere in the range of ±4 A. When the current range of ±9 A was applied at the bipolar power supply, the sweep field ranged from −2.9 to 2.8 mT around B 0 , which was sufficiently wide to acquire EPR spectra of both tooth RIS and the reference signal of 15 N-PDT.  Concepts in Magnetic Resonance Part B, Magnetic Resonance Engineering

Modulation Coil Measurements.
e measured magnetic flux density profiles of field modulation are shown in Figure 5. e peak-to-peak amplitude was 0.38 mT at the geometric center. In addition, to assess the variation in the magnetic field modulation, the line profiles were measured in the ±1 cm region around the geometric center of the magnet. e homogeneity of the modulation field was 5.7%, 3.6%, and 8.0% along the X-axis, Y-axis, and XY plane, respectively. It should be noted that the direction is on the X-axis, and the XY plane is a horizontal midplane between the pole faces. Table 2: Mean value and homogeneity of magnetic flux density evaluated for 2 cm DSV. e magnetic flux density evaluated from the measurement is significantly nonuniform. After smoothing the measured profiles, the homogeneity of the Y-axis and XY plane is 337 and 1600 ppm, respectively. e X-axis is in the direction where the main magnetic field penetrates the two pole faces. e XY plane is a horizontal midplane between the pole faces.  Compared with data from another group where the homogeneity of the modulation coil was 5%, these values were less uniform [25]. However, when the region was confined to 1 cm on the X-axis and 0.2 cm on the Y-axis where only an incisor can be located, the homogeneity was 1.8%.

EPR Spectrum Acquisition.
To fully test the performance of the magnet system integrated with the entire EPR system, it is essential to acquire the EPR spectrum. A tooth was fixed at the geometric center of the developed magnet system. e surface coil of the resonator was contacted to the surface of the tooth (Figure 6(a)). At each end of the magnetic field sweep, the EPR spectrum measured is shown on the computer's display ( Figure 6(b)). Ten sweep data were collected and averaged to produce a spectrum of one tooth.  Figure 5: Measured line profile of modulation magnetic field peak-to-peak amplitude along the X-and Y-axis. e X-axis is in the direction of the main magnetic field, and the Y-axis is in the horizontal plane between poles.
(a) (b) Figure 6: Spectrum acquisition with the developed magnet system: (a) EPR spectra are acquired with a surface coil resonator at the center of the magnet system and (b) the measured signals are collected with the controller system, and the EPR spectrum is acquired. Figure 7 shows EPR spectra acquired from irradiated intact teeth. e spectrum shape was the first derivative of the absorption signal due to magnetic field modulation and phase-sensitive detection. In Figure 7, the left peak was RIS from the tooth, whereas the right one was from the reference material 15 N PDT simultaneously measured. Signal amplitudes of the reference signal have the same level in both spectra of 5 and 30 Gy irradiated teeth. RIS spectra of 5 and 30 Gy teeth were distinguishable in amplitude. e peak-topeak amplitudes of tooth RIS were 0.16 and 0.83 in arbitrary units for 5 and 30 Gy, respectively.

3.7.
ermal Stability of the Magnet. It is known that NdFeB magnets have a temperature coefficient of intrinsic coercivity of approximately −0.10%/°C, which means that the temperature variation of PMs induces the change in the magnetic flux density [32]. e magnetic field shift was observed during long-term EPR measurements. e magnitude of this shift was approximately up to 1.2 mT for the first two hours of operation and then saturated. It seemed due to the temperature rising on PMs mainly by the modulation coils. is magnetic field shift by the heat should be considered when EPR measurements are performed. To prevent the magnetic field shift during the operation, preheating was required when the developed magnet was used. After 2 hours of preheating, the magnetic field shift becomes negligible during further measurements.

Conclusions
A magnet system for in vivo EPR tooth dosimetry was designed and fabricated in this study. e fabricated magnet system satisfied the specifications required to perform in vivo tooth dosimetry. NdFeB PMs were used to generate the main magnetic field, B 0 , which was estimated to be 44.5 mT at the geometric center of the magnet. e field homogeneity was sufficient to be used for EPR tooth dosimetry compared to a known RIS linewidth of tooth spectrum. Furthermore, compared with a 0.26 mT linewidth of tooth RIS, the modulation field was sufficiently strong to measure tooth RIS spectra. e range of the sweep coil was 5.7 mT with ±9 A current. It was wide enough to acquire the full EPR spectra of both RIS and 15 N-PDT. e EPR spectra of the irradiated teeth were successfully acquired using the fabricated magnet system. e RIS of 5 and 30 Gy irradiated teeth was clearly distinguishable.
Data Availability e COMSOL simulation data used to support the findings of this study are included within the article.