Case Reports in Hematology
Volume 2019 (2019), Article ID 8437805, 3 pages
https://doi.org/10.1155/2019/8437805

Case Report

Persistent Mixed Donor Chimerism following Double Umbilical Cord Transplantation in a Patient with T-Cell Prolymphocytic Leukemia

Ronak H. Mistry,¹ Elizabeth O. Hexner,² and James K. Mangan²

¹Department of Internal Medicine, Pennsylvania Hospital of the University of Pennsylvania, 800 Spruce Street, Philadelphia, PA 19107, USA
²Abramson Cancer Center, Perelman Center for Advanced Medicine, University of Pennsylvania, 34th Street and Civic Center Boulevard, 12th Floor South Pavilion, Philadelphia, PA 19104, USA

Correspondence should be addressed to James K. Mangan; jkm3476@yahoo.com

Received 21 May 2019; Accepted 23 August 2019; Published 11 September 2019

Academic Editor: Takashi Sonoki

Copyright © 2019 Ronak H. Mistry et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

T-cell prolymphocytic leukemia (T-PLL) is a rare and aggressive postthymic T-cell neoplasm with an associated survival time of 1 year when left untreated. Current standard of care for T-PLL is with alemtuzumab, followed by allogeneic or autologous stem cell transplant. Little is found in the literature about alternative donor transplantation in T-PLL. Here, we present the case of a patient treated with double umbilical cord blood transplantation, which resulted in initial complete remission. An unusual outcome of this case is that coengraftment of both cords was established. After 16 months, the patient had relapse of the disease, unfortunately, prompting treatment with alemtuzumab and pentostatin, which resulted in remission once again. Here, we report a unique phenomenon whereby single-cord dominance occurred after treatment with these agents, suggesting that anti-T-cell therapy after transplant may help achieve single-unit dominance. A second relapse of the disease occurred six months thereafter, ultimately resulting in the patient’s death, highlighting the aggressive nature of this disease.

1. Introduction

T-cell prolymphocytic leukemia (T-PLL) is a rare postthymic T-cell neoplasm, accounting for approximately 2% of all leukemia cases, with a greater incidence in males beginning in their sixth decade [1]. Typically, untreated T-PLL is associated with a survival of less than 1 year [2]. The aggressive nature of this disease, and its rarity, presents both diagnostic and therapeutic challenges to physicians [2]. Current standard of care for T-PLL includes the use of alemtuzumab (Campath-1H), a humanized IgG1 antibody specific for the CD52 antigen which is highly expressed on normal and malignant lymphocytes [1, 3]. Thereafter, postremission therapy with either autologous or allogeneic stem cell transplant (SCT) has been associated with disease-free and long-term survival, with a median overall survival of 48 months for both allogeneic and autologous SCT, compared to 20 months for those patients who received alemtuzumab alone [1]. There is a paucity of data on alternative donor transplantation in T-PLL. A Japanese group reported the first case of an umbilical cord blood transplant for T-PLL in 2005 [4]; however, we cannot find subsequent reports of umbilical cord blood transplants for this disease. Accordingly, we report here a second case of umbilical cord transplantation used for T-PLL, employing two umbilical cord units this time. Double umbilical cord blood transplant (dUCBT) is increasingly utilized in adults as it appears to improve outcomes, though almost inevitably only one cord blood unit engrafts, a phenomenon known as single-unit dominance [5]. This case is notable for successful engraftment and complete remission that lasted 16 months despite the presence of residual disease at the time of reduced-intensity transplant. An unusual feature of this case was persistent coengraftment of both umbilical cord units, which can be associated with relapse [6]. Persistent coengraftment is rare in dUCBT and has been reported in a small number of case reports [7]. Following relapse 16 months after transplant, a second remission was achieved with a repeat course of alemtuzumab and pentostatin, which lasted 6 months before relapse occurred again. Unbalanced and dynamic coengraftment endured until frank relapse, when the unit contributing less was no longer detectable. This case illustrates the tenuous nature of complete remissions which involve coengraftment after dUCBT. It also demonstrates that dUCBT can result in complete remissions in T-PLL, though the prognosis of patients remains grim with or without allogeneic SCT. We present details of our case below.

2. Case Presentation

A 59-year-old female presented to the local emergency department with reports of fatigue and shortness of breath. The patient was observed to have a WBC >400,000/μL, in addition to pitting edema at the ankles and scattered petechiae. Peripheral smears indicated a marked leukocytosis with atypical blast-like cells. A bone marrow biopsy showed extensive involvement with a T-cell lymphoproliferative disorder with the following immunophenotypes: CD2+, CD4+, CD5+, CD7+, cytoplasmic CD3+, and CD52+, with loss of membrane-associated CD3. Fluorescence in situ hybridization (FISH) indicated a loss of the TCL1 locus, consistent with cyotgenetics showing monosomy 14, as well as numerous other abnormalities (complex and monosomal karyotype), including an addition of 22q. CT imaging indicated splenomegaly and multiple enlarged lymph nodes. Collectively, these findings were diagnostic of T-PLL. This patient was started on intravenous alemtuzumab on a Monday, Wednesday, Friday regimen, initially with a dose escalation of 3 mg on day 1, 10 mg on day 3, and 30 mg on day 5. She continued on 30 mg intravenous alemtuzumab three times weekly for 12 weeks, consistent with current treatment protocols [3].

After four weeks of therapy, the anticipated decrease in WBC was not observed. Therefore, pentostatin was added at 4 mg/m² per week, as previously described [8]. With the addition of pentostatin, bone marrow biopsy indicated complete morphologic and cytogenetic remission. An allogeneic SCT was planned, but since she had neither a related nor a suitable unrelated donor, umbilical cord blood grafts were selected. The grafts were both HLA matched at 5/6 loci. A pretransplant bone marrow biopsy demonstrated a normocellular marrow with trilineage hematopoiesis and 5–10% residual T-PLL cells. Shortly thereafter, the patient underwent reduced intensity dUCBT, conditioned with fludarabine, cyclophosphamide, and total body irradiation [9]; cyclosporine and mycophenolate mofetil were used for graft-versus-host disease (GVHD) prophylaxis. She received 4.2 × 10⁷ total nucleated cells/kg (TNC/kg) from cord 1 (female) and 2.4 × 10⁷ TNC/kg from cord 2 (male). Neutrophil engraftment occurred on day +18, and no signs or symptoms of GVHD ensued. One year after transplantation, a biopsy showed a normocellular marrow (40%) with trilineage hematopoiesis and no overt signs of residual or recurrent T-PLL. Chimerism studies indicated 68% donor 1 and 30% donor 2. A summary of her engraftment analysis is provided in Table 1.

Table 1: Postengraftment analysis following double UCB transplantation, prior to relapse.

Time post-UCB transplant

Donor 1 (female) (%)
Donor 2 (male) (%)

Day +35
Whole blood
48
40

T-cell subset
>90
<10

Day +141
Whole blood
68
30

T-cell subset
67
17

Day +251
Whole blood
70
27

T-cell subset
56
23

12 months
Whole blood
72
21

T-cell subset
49
44

21 months
Whole blood
95
5

T-cell subset
79
13

Sixteen months after transplant, she began to experience increased fatigue and developed new leukocytosis. Flow cytometry confirmed a second relapse of the disease. Soon thereafter, retreatment with alemtuzumab and pentostatin began. The patient became profoundly cytopenic following her alemtuzumab and pentostatin therapy. Bone marrow biopsy in mid-October confirmed a third remission, with 99% donor cells, though again with both grafts represented 80% donor 1 and 19% donor 2. She remained in complete remission with incomplete platelet recovery; the percentage of donor 1 increased to 95% and percentage of donor 2 dropped to 5% during this time. Unfortunately, however, after a remission that lasted six months, the patient relapsed for a third time and was refractory to attempts at salvage therapy. She expired after surviving for 29 months following her transplant.

3. Discussion

The phenomenon of single-unit dominance has been well described [5]. It is thought to be mediated by rejection of one graft by T cells in the dominant graft [10], though predicting which cord will predominate is not possible [7]. Coengraftment and mixed donor/recipient chimerism have been associated with relapse [6]. Following successful treatment of relapse after dUCBT with alemtuzumab and pentostatin, no recipient chimerism was detectable, while donor 1 engraftment peaked at 95%, but donor 2 still persisted (5%). It is interesting that posttransplant therapy with alemtuzumab and pentostatin brought the patient closer to achieving single-unit dominance, which raises the possibility that anti-T-cell therapy after transplant may promote single-unit dominance and potentially protect against relapse. There is clinical evidence supporting the idea that the presence of specific T-cell populations after transplant can mediate the dominance of one cord blood unit over the other, and preclinical data demonstrating that depletion of immune cell fractions within a graft strongly influences coengraftment [10]. Additional research will be needed to fully elucidate the mechanisms of single-unit dominance after dUCBT and to learn whether immune manipulation in a way that promotes single-unit dominance can reduce relapse risk. This case does illustrate, however, that dUCBT is feasible for T-PLL, and postremission dUCBT should be considered for patients with T-PLL who lack available suitably matched related or unrelated donors. This patient remained relatively responsive to salvage therapies even after relapsing following dUCBT and survived for 12 months following her initial posttransplant relapse and 29 months after transplant. This exceeds the anticipated survival for patients treated with alemtuzumab-based therapies alone without transplant, particularly given detectable residual disease at the time of transplant and treatment with a reduced intensity conditioning regimen.

Conflicts of Interest

The authors declare no conflicts of interest regarding the publication of this article.

References

A. Collignon, A. Wanquet, E. Maitre, E. Cornet, X. Troussard, and T. Aurran-Schleinitz, “Prolymphocytic leukemia: new insights in diagnosis and in treatment,” Current Oncology Reports, vol. 19, no. 4, 2017.

M. Damlaj, N. H. Sulai, J. L. Oliveira et al., “Impact of alemtuzumab therapy and route of administration in T-prolymphocytic leukemia: a single-center experience,” Clinical Lymphoma Myeloma and Leukemia, vol. 15, no. 11, pp. 699–704, 2015.

A. Österborg, R. Foà, R. F. Bezares et al., “Management guidelines for the use of alemtuzumab in chronic lymphocytic leukemia,” Leukemia, vol. 23, no. 11, pp. 1980–1988, 2009.

T. E. Tanimoto, A. Hirano, K. Nagafuji et al., “Mismatched unrelated cord blood transplantation in a patient with T-cell prolymphocytic leukemia,” Leukemia, vol. 19, no. 4, pp. 679–681, 2005.

N. S. Majhail, C. G. Brunstein, and J. E. Wagner, “Double umbilical cord blood transplantation,” Current Opinion in Immunology, vol. 18, no. 5, pp. 571–575, 2006.

P. Ramirez, J. E. Wagner, T. E. DeFor et al., “Factors predicting single-unit predominance after double umbilical cord blood transplantation,” Bone Marrow Transplantation, vol. 47, no. 6, pp. 799–803, 2012.

H. Hashem and H. M. Lazarus, “Double umbilical cord blood transplantation: relevance of persistent mixed-unit chimerism,” Biology of Blood and Marrow Transplantation, vol. 21, no. 4, pp. 612–619, 2015.

J. Mercieca, E. Matutes, C. Dearden, K. MacLennan, and D. Catovsky, “The role of pentostatin in the treatment of T-cell malignancies: analysis of response rate in 145 patients according to disease subtype,” Journal of Clinical Oncology, vol. 12, no. 12, pp. 2588–2593, 1994.

J. N. Barker, “Rapid and complete donor chimerism in adult recipients of unrelated donor umbilical cord blood transplantation after reduced-intensity conditioning,” Blood, vol. 102, no. 5, pp. 1915–1919, 2003.

C. H. J. Lamers, R. Wijers, C. A. M. van Bergen et al., “CD4+ T-cell alloreactivity toward mismatched HLA class II alleles early after double umbilical cord blood transplantation,” Blood, vol. 128, no. 17, pp. 2165–2174, 2016.