Psychological and Physiological Relaxation Induced by Nature-Working with Ornamental Plants

Objective. Nature has a significant impact on general well-being. However, till date, little psychophysiological evidence is available on the benefits of nature-based activities in adults. e primary goal of this study was to determine the physiological and psychological benefits of horticultural activity in adults. e participants were instructed to perform the making of a flower basket (horticultural activity) and a computer task (i.e., control activity) was compared. Methods. A total of 40 Chinese females (mean age 22.2 ± 0.9 years) participated in this experiment. e Blood pressure, the State-Trait Anxiety Inventory (STAI), and the electroencephalograms (EEGs) were used to measure the participants’ psychophysiological responses. Results. Analysis of the STAI data showed a lower anxiety score a er performing the horticultural activity than a er performing the control activity. Furthermore, in the EEG evaluation, variations in the brainwaves were observed a er both activities. Conclusions. e study results suggest that horticultural activity induced physiological and psychological relaxation in adults.


Introduction
Humans are considered to be entirely dependent on nature for their emotional, cognitive, and physical needs [1][2][3]. However, whether nature is beneficial for mental health remains under investigation by scientists at many educational institutions [4]. In the 1970s, scholars investigated the psychological role of nature applied psychometric measures to explore the eco-friendly perceptions and cognitive states of human subjects in natural surroundings [5,6]. However, in the 1970s, some scholars began to examine the effects of nature on the participants' psychological and "biological responses to various environments" [7][8][9][10]. Studies have found that contact with plants can have both psychological and physiological benefits [7,11,12]. Furthermore, involvement in nature and/ or wilderness settings can help with stress management and can be healing [13]. In addition, humans who spend time in natural environments are healthier and more mentally active [14]. Currently, people are increasingly living in urban places and spending more time indoors away from nature [15]. Many mental health problems, such as depression and stress, are believed to occur from a lack of exposure to nature or from living in an artificial world [16]. Furthermore, recent advancements in technology are also considered to be hurdles because most adult populations are connected to electronics, such as mobile phones and tablet devices, and they are losing their connection to the natural environment. Moreover, this dispersal of information technology causes considerable stress, such as technostress [17], which is a new disease of adaptation that is caused by an inability to cope with new computer technologies in a healthy way. erefore, there is a growing need to reconnect adults to the healing powers of natural environments. People also receive benefits from plants in natural or vegetative surroundings in both direct and indirect ways such as lower blood pressure, increased productivity and attentiveness, reduced mental fatigue, and stress and fewer reports of illness [18]. Active contacts such as contact with plants have been shown to provide psychophysiological benefits, including improved social interaction, increased self-esteem, and reduced stress levels [5]. Limited numbers of studies have described the clinical effects of horticultural therapy; although, interactions with nature have been shown to have physical, emotional, social, and cognitive benefits that help reduce stress [19]. Few studies have examined the effects of indoor plants that are relevant to the well-being and effectiveness of office workers. ese outcomes include task performance, room assessments psychophysiological stress responses, and emotional states [20][21][22][23]. Additionally, some experiments have explored attitudes towards plants in the office [24], and the effects of plants on the discomfort and health symptoms that are associated with sick building syndrome [25][26][27]. However, the findings from experiments that were conducted in either simulated or laboratory settings may not generalize well to real office settings. Additionally, to date, there is a lack of scientific research on EEG regarding the effects of plants in adults.

Overview of Electroencephalograms (EEG) and Brainwaves
Richard Caton (1875) discovered the existence of electrical currents in the brain. Subsequently, in 1924, Hans Berger was the first to record the electrical currents inside the brain. He used EEGs to explain electrical brain activity and discovered the alpha wave (8)(9)(10)(11)(12), which is also known as Berger's wave [28]. In 1934, Adrian and Matthews introduced new ideas regarding human brainwaves. During the 1950's, EEG technology was successfully applied in all fields of science including neurosurgery, neurology, and cognitive science. Finally, in 1964, Dr. Gray Walter connected electrodes directly to the motor areas of a human brain to investigate brainwave activity. e human brain consists of hundreds of billions of brain cells that are called neurons. ese neurons have axons that can transmit signals through neurotransmitters to the dendrites of other neurons, which then lead to an electrical polarity change inside the neuronal axon of the receiving neuron. is polarity change can be recorded by using EEGs [29,30]. e EEG signals originate from the inhibitory (hyperpolarizing) and excitatory (depolarizing) post-synaptic potentials of the pyramidal neurons, which are present in the lower portion of the cerebral cortex [31,32]. EEGs represent the electrical activity inside the brain and provide valuable information regarding various tasks that occur in the brain. In the medical field, EEGs are primarily used for various diagnoses, such as cerebrovascular or structural brain diseases, dementia, epilepsy, disturbances of consciousness, brain death, and other psychological disorders. However, EEGs are also used to investigate electrical brainwave responses [32,33], alpha waves occur when an individual is in a relaxed state, whereas beta waves occur during alert and focused states [21]. Increased alpha brain activity is connected more to a relaxed state than to a condition of stress [34]. Recent advancements in cognitive neuroscience, information technology, and other invasive or noninvasive brain-signal capturing devices allow us to directly explore the human brain [35]. e NeuroSky Mind Wave is an alternative nonmedical instrument that was designed by the NeuroSky Company and can be used as a computerhuman interface to record human brainwave activity. e brain-computer interface (BCI) system is a direct communication pathway between the brain and an external device [36].
is technology is used in various computer-supported applications, such as video games, research so ware, and human health perspectives. e NeuroSky Mind Wave contains a single recording probe, and the signals are typically received through an electrode that is placed on the le side of the forehead above the eyes (Fp1 position) [37]. Compared with other professional instruments, this device is simple to operate, inexpensive, and has 86% accuracy. e major components that are built into the Mind Wave headset include a Bluetooth with a computer and a ink Gear generic chip, which contains signal data that can be visualized by a computer a er processing. e NeuroSky Mind Wave headset provides unfiltered brainwave data and can be used by scientists to perform their own measurements. ere is a little EEG information on the positive effects of horticultural activity in modern adults. erefore, the goals of this study were to investigate the psychophysiological effects of horticultural activity in adults.

Materials and Methods
3.1. Participants. 40 Chinese female students (mean age 22.2 ± 0.9 years; mean weight 52.76 ± 9.80 kg; mean height 160.68 ± 5.87 cm) at the Sichuan Agricultural University in China participated in the experiment. None of the subjects had a history of psychological disorders. All drugs were strictly prohibited throughout the experiment. e experiments were performed in silence in a laboratory room at the College of Landscape Architecture. Prior to the experiments, the participants were fully informed of the methods of this study, and written informed consent was obtained. is experiment was conducted with the approval of the Ethics Committee of the College of Landscape Architecture, at the Sichuan Agricultural University, China.

Materials.
Arranging flower baskets is a typical horticultural activity and was selected for this study; this activity involved the use of real flowers (i.e., roses (Rosa hybrid), chrysanthemums (Chrysanthemum morifolium), bamboos (Fargesia), lilies (Lilium candidum), and carnations (Dianthus caryophyllus) of approximately the same size and weight. A basic method regarding the making of flower baskets was taught to each participant prior to the experiments so that they could work more smoothly.

Protocol.
e subjects were randomly divided into two groups. On the first day of the experiment, group A performed the horticultural activity (making flower baskets) by using real flowers, and group B performed the control activity (downloading research papers) by using a computer; the control activity is a typical computer activity, that also needs constant physical activity, similar to the horticultural task ( Figure 1). On the second day, the groups switched activities. Both activities were performed in a seated position for 12 min.

3.4.
Measurements. An EEG headset was attached to the subjects' heads in the waiting room. en, the subjects entered the experimental room. A er a 5-min rest in a seated position, the subjects performed their assigned tasks, i.e., either the horticultural activity that uses real flowers or the computer task, for 12 min. EEGs were continuously measured during each task with a Mind Wave EEG headset (NeuroSky Mind Wave Beijing Oriental Creation Technology Co., LTD, China). Typically, an EEG records the brainwave activity inside the brain from the Fp1 position above the eye, which accords with the American Electroencephalographic Society's (1994) 10-20 system of electrode placement. In addition, the EEG setup consisted of four essential parts, namely, (1) a headband, (2) an ear-clip, (3) a sensor arm that contains the EEG electrode, and (4) a Bluetooth device. e Blood pressure and the pulse rate data were recorded before and a er the tasks, by using a digital blood pressure device (Omron, HEM-7011, China-7201 type upper arm type intelligent electronic sphygmomanometer). e EEG data were recorded before and throughout the experiment (12 min), and the alpha and beta brainwaves were compared between the two conditions to determine the participant's relaxation and attention levels in the two conditions. e measurements were obtained with a frequency of 512 Hz, which means that values were obtained every second. e raw EEG data that include high alpha and high beta brainwaves were collected at 1-minute intervals at each experimental site and were compared between the two conditions. e higher values of experimental data show the participants in a more relaxed and alert condition. Relaxed wakefulness is a condition that is used for alpha activity during its predominant state [38]. e feelings that the participants experienced during the experiment were examined by using the State-Trait Anxiety Inventory (STAI) [39], a self-rated questionnaire that was completed before and a er the tasks. e (STAI) consists of twenty questions (e.g., "I feel nervous"; "I feel relaxed"; "I feel frightened", etc.). e participants answered each of the twenty items according to how they felt before and a er performing both activities by using the following four-point scale: 1 = moderately, 2 = not at all, 3 = very much, and 4 = somewhat.

Statistical
Analysis. Statistical analyses were conducted by using SPSS 16.0 (SPSS Inc., Chicago, IL, USA). A paired t-test was used to determine the blood pressure differences between the two groups. A one-way analysis of variance (ANOVA) was used to compare the horticultural and control groups separately to study the significant differences in the pre-and posttests' alpha and beta mean values. A two-way repeated measure ANOVA was used to check whether there were any statistically significant differences between the horticultural and control groups' pretests and posttests changes in the alpha and beta mean values. For the physiological data, statistical significance was defined as 푃 < 0.05. For the psychological data, a Wilcoxon signed-rank test was used, and statistical significance was defined as 푃 < 0.01.

Results
Significant differences (푃 < 0.05) were observed in the systolic and diastolic blood pressure (mmH between the horticultural and control groups (109.6 ± 6.8; 116.8 ± 11.8; 푃 = 0.027; 69.4 ± 5.3; 72.8 ± 6.3; 푃 = 0.058). However, no significant difference was found in the pulse rate (BPM) between the two groups (74.0 ± 7.2; 75.0 ± 8.5; 푃 = 0.067) (Figure 2). No significant differences were observed between the horticultural group and the control group in the participants' relaxation, based on a one-way ANOVA that compared the alpha mean values between the groups on the pretest (퐹 = 1.21, 푃 = 0.22). However, the results of the posttest indicated that the control group had a drop in the participants' relaxation based on the alpha mean values. Furthermore, a one-way ANOVA indicated that this drop in the alpha mean values by the control group over the pre-and posttests' duration was significant (퐹 = 2.84, 푃 < 0.01). In contrast, the horticultural group had a significant increase in the participants' relaxation, based on a one-way ANOVA of the alpha mean values over the pre-and posttest duration (퐹 = 2.02, 푃 = 0.0038).
However, a two-way ANOVA indicated that the pre-and posttests variations in the participants' relaxation, based on

Discussion
In this experiment, we investigated the relaxation effects of a horticultural activity by measuring and comparing the participants' psychological and physiological responses between two tasks: a flower basket making activity by using real flowers and a computer task. Blood pressure was significantly decreased in the horticultural activity than in the control activity. e lower blood pressure indicates that interaction with plants has a beneficial effect on adults. Studies on human-plant interaction have indicated that direct or indirect contact with plants or natural surroundings can have a greater beneficial effect on people's well-being [5,10,40]. A comparison of blood pressure finds that the horticultural activity has a positive effect on mental stress that increases when a human is exposed to a stressor. e findings of this experiment agree with a previous study that reported that plant activity reduces stress [41]. e findings of the EEGs show that plants have a greater relaxing effect on the brain by enhancing brain activity, which changes when a person connects to a stressor. In this experiment, the value of the alpha brainwaves increases a er the horticultural activity, while a lower alpha brainwave value is observed a er the control activity. e increase in alpha brainwaves clearly shows that the participants were mentally relaxed; however, lower alpha brainwaves indicated the condition of stress. Studies on EEGs have reported that an increase in mental workload caused the alpha waves to decrease or disappear [42]. Additionally, alpha waves are associated with relaxation and mental coordination [43,44]. Previous plant studies with EEGs have reported that an increase of satisfaction or relaxation causes increased alpha and beta brainwave activity [45]. Moreover, another experiment regarding horticultural activities with and without plants reported that the ratio of both the alpha and beta brainwaves significantly increased when subjects closed their eyes only in the plant group [46]. us, plants have strong effects on the brain by changing the brainwave activity. Furthermore, the value of the beta brainwaves increases a er the horticultural activity, while lower beta brainwaves are observed a er the control activity. the alpha mean values, for the horticultural group were significantly greater than the pre-and posttests variations in the participants' relaxation, based on the alpha mean values, for the control group (퐹 = 16.2, 푃 < 0.01). Similarly, no significant differences were observed between the horticultural group and the control group in the participants' attention, based on a one-way ANOVA that compared the beta mean values between the groups on the pretest (퐹 = 1.13, 푃 = 0.30). Moreover, the results of the posttest indicated that the control group had a drop in the participants' attention based on the beta mean values. Furthermore, a one-way ANOVA indicated that this drop in the beta mean values by the control group over the pre-and posttests' duration was not significant (퐹 = 0.89, 푃 = 0.61). Furthermore, the results of the posttest indicated that the horticultural group had an increase in the participants' attention based on the beta mean values. However, based on a one-way ANOVA, the horticultural group beta mean values over the pre-and posttest durations were not significant (퐹 = 0.81, 푃 = 0.72). A two-way ANOVA indicated that the pre-and posttests variations in the participants' attention, that were based on the beta mean values, for the horticultural group were significantly greater than the pre-and posttests variations in the participants' attention, that were based on the beta mean values, for the control group (퐹 = 13.01, 푃 = 0.001). According to the STAI data, the subjects' elings during the horticultural task (which used real flowers) were different from their feelings during the control task (computer task). ere were significant differences between the two tasks in the total anxiety score, which was measured before and a er the completion of the 12-min. tasks. As shown in Figure 3, the level of anxiety a er the horticultural activity was significantly lower than the level of anxiety a er the control activity (Horticultural group; 38.5 ± 4.5; Control group; 44.4 ± 4.4; 푃 = 0.001. However, there was no significant difference in the baseline (before the activities), which suggests that the horticultural activity may have a better effect in lowering anxiety in adults.

Conflicts of Interest
Authors declare that they have no conflicts of interests.

Authors' Contributions
Jiang Tao and Ahmad Hassan worked equally as a first author and were responsible for data obtaining, statistical inquiry and article development. Prof. Chen Qibing and prof. LIU YINGGAO supervised the experiment. Guo Li, Mingyan Jiang, Deng Li, Li Nian, Lv Bing-Yang, Zhong Ziqin helped during the experiment. All authors read and approved the final manuscript. e increase in beta brainwaves clearly shows that the participants were highly alert; however, lower beta brainwaves indicate a lack of attentiveness or stress. Increases in beta waves correlate with a highly alert state of mind, and decreases correlation with a drowsiness state [47]. A study by Monina Islarm reported that an EEG could be used to determine emotions. In his study, both the alpha and beta waves are active during a workload, and when the workload on the brain increases, beta wave activity then increases. e study shows that when people use more focus on a specific task, the ratio of the beta power increases [48]. Additionally, beta waves are fast wave activity and are used to engage in decision making and mental tasks [49] such as attending lectures in a classroom or solving problems. Both alpha and beta waves naturally appear during the active and resting conditions. Our behavior, emotions, and thoughts are the impressions of neuronal activity inside the brain.
e appearance of brain waves shows the neuronal electrical activity within the brain. EEGs record these electrical fluctuations and represent them as waves. Brain waves with a slower frequency range appear when we feel dreamy or tired. In contrast, higher frequencies are dominant when we feel excited or alert [50]. Finally, the STAI results also show that the control activity may have negative effects on the mood state. e results show that the participants felt more stress a er performing the control activity than a er performing the horticultural activity. Studies have reported that active interaction with plants has positive effects on mood [51,52].

Conclusion
e findings of this experiment suggest that horticultural activity lowers blood pressure and, increases alpha and beta brainwave activity as compared with mental task. Future studies could determine whether the positive effects of horticultural activity last over longer time periods with more diversified groups with large sample. Furthermore, a comparison of horticultural therapy with a pet therapy (control group) could help researchers better understand the effectiveness of plants and should be tested in future studies.
Data Availability e research data used to support the findings of this study are available from the corresponding author upon request.

Ethical Approval
Subject's written informed consent was obtained. is study arranged with the approval of the local Ethics Committee College of Landscape Architecture, Sichuan Agricultural University, China.

Consent
Subject's written informed consent was obtained for the publication of images.