Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 4032820, 14 pages
https://doi.org/10.1155/2017/4032820

Research Article

Rapid Screening of Chemical Constituents in Rhizoma Anemarrhenae by UPLC-Q-TOF/MS Combined with Data Postprocessing Techniques

Lanlan Shan, Yuanyuan Wu, Lei Yuan, Yani Zhang, Yanyan Xu, and Yubo Li

Tianjin State Key Laboratory of Modern Chinese Medicine, School of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China

Correspondence should be addressed to Yanyan Xu; xyytjutcm@163.com and Yubo Li; yaowufenxi001@sina.com

Received 27 February 2017; Revised 11 May 2017; Accepted 4 June 2017; Published 25 September 2017

Academic Editor: Attila Hunyadi

Copyright © 2017 Lanlan Shan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract.

Rhizoma Anemarrhenae, a famous traditional Chinese medicine (TCM), is the dried rhizome of Anemarrhena asphodeloides Bge. (Anemarrhena Bunge of Liliaceae). The medicine presents anti-inflammatory, antipyretic, sedative, and diuretic effects. The chemical constituents of Rhizoma Anemarrhenae are complex and diverse, mainly including steroidal saponins, flavonoids, phenylpropanoids, benzophenones, and alkaloids. In this study, UPLC-Q-TOF/MS was used in combination with data postprocessing techniques, including characteristic fragments filter and neutral loss filter, to rapidly classify and identify the five types of substances in Rhizoma Anemarrhenae. On the basis of numerous literature reviews and according to the corresponding characteristic fragments produced by different types of compounds in combination with neutral loss filtering, we summarized the fragmentation patterns of the main five types of compounds and successfully screened and identified 32 chemical constituents in Rhizoma Anemarrhenae. The components included 18 steroidal saponins, 6 flavonoids, 4 phenylpropanoids, 2 alkaloids, and 2 benzophenones. The method established in this study provided necessary data for the study on the pharmacological effects of Rhizoma Anemarrhenae and also provided the basis for the chemical analysis and quality control of TCMs to promote the development of a method for chemical research on TCMs.

1. Introduction

Rhizoma Anemarrhenae is one of the traditional Chinese medicines (TCMs) that are commonly used for clearing heat and purging fire, nourishing yin, moistening dryness, reducing anxiety and thirst, and promoting excretion. This TCM is the dried rhizome of the Liliaceae Anemarrhena genus and has been widely used for clinical treatment of febrile disease, high fever and thirst, cough and asthma, osteopyrexia and fever, and other diseases [1, 2]. The pharmacologically active components of Rhizoma Anemarrhenae include steroidal saponins, flavonoids, phenylpropanoids, alkaloids, and benzophenones, with saponins being the major constituents [3–5]. At present, the analytical methods of TCM ingredients mainly include thin layer chromatography (TLC), gas chromatography (GC), high-performance liquid chromatography (HPLC), and ultraperformance liquid chromatography/time-of-flight mass spectrometry (UPLC-Q-TOF/MS), among others. As a comprehensive analysis technique, UPLC-Q-TOF/MS has been increasingly applied for the qualitative and quantitative analysis of components in TCMs and compound preparations [6–11]. However, this method displays several disadvantages, including complicated and time-consuming data processing. Therefore, a newly developed method to rapidly identify components in TCMs is urgently needed to improve the efficiency and accuracy of the qualitative analysis and to promote the development of modernization and internationalization of TCMs.

With the rapid development of modern technology, data postprocessing techniques, mainly including characteristic fragments filter (CFF) and neutral loss filter (NLF), play increasingly important roles in TCM research [12–17]. CFF is based on compounds with the same or similar mother nucleus structures that can produce characteristic fragments to determine a certain type of compound under the same mass spectrometric conditions. NLF is based on a certain type of compounds that frequently lose the same neutral fragments by energy collision splitting. According to the loss of these neutral fragments, substances with a class of characteristic substituted groups can be briefly classified [18–22]. CFF is favorable for the rapid screening of similar compounds, whereas NLF helps further confirmation of compounds. Thus, the combination of both techniques markedly reduces qualitative analysis difficulty and was gradually applied in the field of pharmaceutical analysis.

In recent years, UPLC-Q-TOF/MS is widely used to analyze constituents in Rhizoma Anemarrhenae [23, 24]. In this study, Rhizoma Anemarrhenae was selected as an example to establish an efficient approach for the rapid screening of chemical components by using UPLC-Q-TOF/MS in combination with data postprocessing techniques. First, through reading and integration of abundant information from the literature, we summarized the fragmentation patterns of five kinds of compounds (steroidal saponins, flavonoids, phenylpropanoids, alkaloids, and benzophenones) in Rhizoma Anemarrhenae. Secondly, a full spectrum scan was performed by UPLC-Q-TOF/MS. Finally, the compounds were characterized based on the CFF and NLF rule. Compared with traditional methods, the study could rapidly classify and identify a certain type of compounds. The method of UPLC-Q-TOF/MS coupled with data postprocessing techniques not only partly overcame the difficulty of rapid classification and identification of complex components of TCM but also laid the foundation for the rapid development of composition identification and provided an analytical method for the further research and development of TCMs.

2. Experimental

2.1. Sample Preparation

Prepared Rhizoma Anemarrhenae was accurately weighed (5 g) and extracted twice with 50 and 40 mL of 75% methanol, respectively, and each reflux time was 1 h. The extracts were filtered, merged, and then concentrated to 0.01 g/mL. The concentrated solution was filtered using a 0.22 μm syringe filter, with 2 μL injected for UPLC-Q-TOF/MS analysis.

2.2. UPLC-Q-TOF/MS Conditions

Chromatographic separation was conducted on a Waters Acquity UPLC Class I series, which consisted of a quatpump, an autosampler, a DAD detector, and a column compartment. All separation was carried out on a Waters ACQUITY UPLC BEH C₁₈ column (100 mm × 2.1 mm, 1.7 μm particle size) with a set column temperature of 35°C. The solvent flow rate was 0.3 mL/min, and 2 μL of sample solution was injected in each run. The mobile phase was composed of water (solvent A) and acetonitrile (solvent B). The gradient elution program employed was as follows: 0–0.5 min, 2%–5% B; 0.5–3 min, 5%–20% B; 3–5 min, 20%–22% B; 5-6 min, 22%-22% B; 6–10 min, 22%–38% B; 10–14 min, 38%–50% B; 14-15 min, 50%-50% B; 15–18 min, 50%–100% B; 18–20 min, 100%-100% B; 20-21 min, 100%–2% B; 21–23 min, 2%-2% B.

UPLC was coupled to a Q-TOF-MS system (Waters, USA) equipped with an electrospray ionization source (ESI) for scanning samples in negative ion modes. Ultrahigh purity helium (He) was used as the collision gas, and high-purity nitrogen (N₂) was used as nebulizing gas. The conditions of ESI source were as follows: drying gas temperature: 325°C; drying gas flow rate: 10 mL min⁻¹; desolvation gas flow: 600 L/h; capillary voltage: 2.0 kV; collision energy: 20–40 eV; scan spectra from m/z 50–1500; nebulizing gas pressure: 350 psi. Leu-enkephalin ions at m/z 556.2771 and 554.2615 were used to ensure accuracy in spectral acquisition.

2.3. Data Analysis

Original data were analyzed using Masslynx (Waters, USA) software 4.1. to detect and align the peaks. Data were processed and converted to an Excel format containing complete information on mass, retention time, and peak area of the samples. Target compounds were obtained by output data processing.

3. Results and Discussion

3.1. Method Development

The chemical constituents of Rhizoma Anemarrhenae are complex and diverse, but similar kinds of compounds exhibit certain similarities in chemical structures. We summarized the fragmentation patterns of compounds in Rhizoma Anemarrhenae according to the mass spectrometric behavior; that is, the same type of compounds with the same or similar public skeleton usually show the same fracture mode to produce the same CFs. The method that uses CFs to determine a certain type of compounds is called CFF. For example, phenylpropanoid compounds can produce characteristic fragment ions at m/z 107 [C₇H₇O]⁻ and 91 [C₇H₇]⁻ in negative ion mode. The ions could be screened from the total ion current chromatograms according to their CFs and then identified according to specific fragments and molecular ion peaks. In MS collision-induced dissociation, the differences between molecular ion peaks and high mass-to-charge ratio fragment peaks also play an important role in component identification. The method that uses NLs to screen compounds is called NLF. Mangiferin could easily lose neutral fragments at m/z 90 (C₃H₆O₃) and 120 (C₄H₈O₄) and could be filtered according to the two characteristic neutral fragments. CFF and NLF perform an increasingly important role in TCM research. Therefore, the complex components in Rhizoma Anemarrhenae can be rapidly and accurately classified and identified by combining UPLC-Q-TOF/MS with data postprocessing techniques (CFs and NLs). Finally, Based on the results of our study and those described in relevant and previous experiments, the rules of CFF and NLF regarding the five categories were established (shown in Table 1).

Table 1: Characteristic fragments and neutral loss information of chemical substances in the Rhizoma Anemarrhenae.

Compound classification
Subclass
Characteristic fragments
Neutral loss

Steroidal saponins

    Spirostanol saponins

Type I saponins
273 C₁₉H₂₉O]⁻, 255 C₁₉H₂₇]⁻, 301 C₂₁H₃₃O]⁻, 283 C₂₁H₃₁]⁻
162 (C₆H₁₀O₅), 132 (C₅H₈O₄)
18 (H₂O)
142 (C₈H₁₄O₂)

Type II and type III saponins
289 C₁₉H₂₉O₂]⁻,
317 C₂₁H₃₃O₂]⁻

Type IV saponins
271 C₁₉H₂₇O]⁻, 253 C₁₉H₂₅]⁻,
299 C₂₁H₃₁O]⁻, 281 C₂₁H₂₉]⁻

    Furostan saponins 
    M-H-OH]⁻ 
    M-H-OCH₃]⁻
Type V saponins
269 C₁₉H₂₅O]⁻, 251 C₁₉H₂₃]⁻

Flavonoids
Mangiferin-type flavonoids

90 (C₃H₆O₃),
120 (C₄H₈O₄)

Chalcone-type flavonoids
119 C₈H₇O]⁻ or 146 C₉H₆O₂]⁻

Flavanones
119 C₈H₇O]⁻,
165 C₈H₅O₄]⁻

Homoisoflavonoid
133 C₉H₉O]⁻

Icariin-type flavonoids
367 C₂₁H₁₉O₆]⁻
162 (C₆H₁₀O₅), 132 (C₅H₈O₄)

Phenylpropanoids
coumarins
107 C₇H₇O]⁻,
91 C₇H₇]⁻

Benzophenones

94 (C₆H₆O)
44 (CO₂)

Alkaloids
Amide alkaloids

119 (C₈H₇O)
15 (CH₃)

A full scan was conducted in both positive and negative modes to analyze the Rhizoma Anemarrhenae extract, and results show that the ESI negative ion mode was simpler, more stable, and easier to interpret compared with the positive ion mode, and the characteristic ions obtained in the negative ion mode could be evidently observed to distinguish the two types of steroidal saponins. Thus, the negative ion mode was used in this study. The full scan MS chromatograms of substances in Rhizoma Anemarrhenae extracts are shown in Figure 1. The fragmentation patterns of the five types of main constituents in Rhizoma Anemarrhenae were summarized, and 32 compounds were identified (shown in Table 2).

Table 2: Identification of the chemical constituents of Rhizoma Anemarrhenae extract by using UPLC-Q-TOF/MS in negative ion mode.

RT
Formula
[M-H]⁻
Experimental m/z
ppm
Fragment ions
Chemical name
Chemical types
Ref

1
2.72
C₂₅H₂₈O₁₆
583.1299
583.1299
0.00
583 [M-H]⁻,
421 [M-H-C₆H₁₀O₅]⁻,
331 [M-H-C₆H₁₀O₅-C₃H₆O₃]⁻,
301 [M-H-C₆H₁₀O₅-C₄H₈O₄]⁻,
Neomangiferin
Mangiferin-type flavonoids
[5, 25]

2
3.27
C₁₉H₁₈O₁₁
421.0771
421.0759
2.85
421 [M-H]⁻,
331 [M-H-C₃H₆O₃]⁻,
301 [M-H-C₄H₈O₄]⁻,
Mangiferin
Mangiferin-type flavonoids
[5, 25]

3
3.32
C₁₉H₁₈O₁₁
421.0771
421.0764
1.66
421 [M-H]⁻,
331 [M-H-C₃H₆O₃]⁻,
301 [M-H-C₄H₈O₄]⁻,
Isomangiferin
Mangiferin-type flavonoids
[5, 25]

4
4.48
C₁₃H₁₀O₅
245.0450
245.0454
1.63
245 [M-H]⁻,
151 [M-H-C₆H₆O]⁻,
107 [M-H-C₆H₆O-CO₂]⁻,
Iriflophenone
Benzophenones
[26]

5
4.74
C₄₅H₇₆O₂₀
935.4852
935.4823
3.10
917 [M-H-H₂O]⁻,
773 [M-H-C₆H₁₀O₅]⁻,
289 [M-H-H₂O-3C₆H₁₀O₅-C₈H₁₄O₂]⁻,
271 [M-H-2H₂O-3C₆H₁₀O₅-C₈H₁₄O₂]⁻,
253 [M-H-3H₂O-3C₆H₁₀O₅-C₈H₁₄O₂]⁻,
281 [M-H-3H₂O-3C₆H₁₀O₅-C₆H₁₀O₂]⁻,
Timosaponin E1
Furostanol glycosides
[5, 27]

6
4.79
C₄₅H₇₆O₂₀
935.4852
935.4827
2.67
935 [M-H]⁻,
773 [M-H-C₆H₁₀O₅]⁻,
289 [M-H-H₂O-3C₆H₁₀O₅-C₈H₁₄O₂]⁻,
271 [M-H-2H₂O-3C₆H₁₀O₅-C₈H₁₄O₂]⁻,
253 [M-H-3H₂O-3C₆H₁₀O₅-C₈H₁₄O₂]⁻,
Timosaponin E
Furostanol glycosides
[5, 27]

7
4.85
C₄₅H₇₆O₂₀
935.4852
935.4836
1.71
935 [M-H]⁻,
773 [M-H-C₆H₁₀O₅]⁻,
289 [M-H-H₂O-3C₆H₁₀O₅-C₈H₁₄O₂]⁻,
Timosaponin N
Furostanol glycosides
[5, 27]

8
5.09
C₅₆H₉₄O₂₉
1229.5803
1229.5806
0.24
1211 [M-H-H₂O]⁻,
289 [M-H-H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻,
271 [M-H-2H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻,
253 [M-H-3H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻,
281 [M-H-3H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₆H₁₀O₂]⁻,
Purpureagitoside
Furostanol glycosides
[5, 27]

9
6.32
C₁₇H₁₇NO₃
282.1130
282.1132
0.71
282 [M-H]⁻,
163 [M-H-C₈H₇O]⁻,
Coumaroyltyramine
Amide alkaloids
[28]

10
6.86
C₁₈H₁₉NO₄
312.1236
312.1237
0.32
312 [M-H]⁻,
297 [M-H-CH₃]⁻,
178 [M-H-CH₃-C₈H₇O]⁻,
N-trans-feruloyltyrami-ne or N-cis-feruloyltyramine
Amide alkaloids
[28]

11
7.20
C₄₅H₇₄O₁₉
917.4746
917.4751
0.54
755 [M-H-C₆H₁₀O₅]⁻,
289 [M-H-3C₆H₁₀O₅-C₈H₁₄O₂]⁻,
271 [M-H-3C₆H₁₀O₅-C₈H₁₄O₂-H₂O]⁻,
253 [M-H-3C₆H₁₀O₅-C₈H₁₄O₂-2H₂O]⁻ 
281 [M-H-3C₆H₁₀O₅-C₆H₁₀O₂-H₂O]⁻,
Timosaponin D
Furostanol glycosides
[5, 27]

12
7.49
C₁₄H₁₂O₅
259.0607
259.0630
8.88
259 [M-H]⁻,
165 [M-H-C₆H₆O]⁻,
121 [M-H-C₆H₆O-CO₂]⁻,
2,6,4′-Trihydroxy-4-methoxyBenzophenone
Benzophenones
[26]

13
8.12
C₅₆H₉₂O₂₈
1211.5697
1211.5642
4.54
1211 [M-H]⁻,
1193 [M-H-H₂O,
271 [M-H-H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻,
253 [M-H-2H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻,
Timosaponin H1
Furostanol glycosides
[5, 27]

14
8.26
C₅₆H₉₄O₂₈
1213.5854
1213.5803
4.20
1213 [M-H]⁻,
273 [M-H-H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂,
255 [M-H-2H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻
Timosaponin I1/D1
Furostanol glycosides
[5, 27]

15
8.48
C₄₅H₇₆O₁₉
919.4903
919.4882
2.28
919 [M-H]⁻,
901 [M-H-H₂O]⁻,
301 [M-H-H₂O-3C₆H₁₀O₅-C₆H₁₀O₂]⁻,
273 [M-H-H₂O-3C₆H₁₀O₅-C₈H₁₄O₂]⁻,
255 [M-H-2H₂O-3C₆H₁₀O₅-C₈H₁₄O₂]⁻,
Timosaponin B II or Timosaponin L or (25S)-Officinalisnin-I
Furostanol glycosides
[5, 27]

16
9.60
C₃₉H₆₄O₁₅
771.4167
771.4174
0.91
771 [M-H]⁻,
609 [M-H-C₆H₁₀O₅]⁻,
289 [M-H-2C₆H₁₀O₅-C₈H₁₄O₃]⁻,
271 [M-H-2C₆H₁₀O₅-C₈H₁₄O₃-H₂O]⁻,
253 [M-H-2C₆H₁₀O₅-C₈H₁₄O₃-2H₂O]⁻,
Timosaponin F (C39)
Spirostanol glycosides
[27]

17
10.01
C₄₅H₇₄O₁₈
901.4797
901.4773
2.66
901 [M-H]⁻,
739 [M-H-C₆H₁₀O₅]⁻,
577 [M-H-2C₆H₁₀O₅]⁻,
273 [M-H-3C₆H₁₀O₅-C₈H₁₄O₂]⁻,
255 [M-H-3C₆H₁₀O₅-C₈H₁₄O₂-H₂O]⁻,
Xilingsaponin B
Spirostanol glycosides
[27]

18
10.14
C₄₅H₇₄O₁₈
901.4797
901.4768
3.22
739 [M-H-C₆H₁₀O₅]⁻,
577 [M-H-2C₆H₁₀O₅]⁻,
273 [M-H-3C₆H₁₀O₅-C₈H₁₄O₂]⁻,
255 [M-H-3C₆H₁₀O₅-C₈H₁₄O₂-H₂O]⁻,
Timosaponin B III
Furostanol glycosides
[5, 27]

19
10.23
C₄₅H₇₄O₁₈
901.4797
901.4807
1.11
739 [M-H-C₆H₁₀O₅]⁻,
577 [M-H-2C₆H₁₀O₅]⁻,
273 [M-H-3C₆H₁₀O₅-C₈H₁₄O₂]⁻,
255 [M-H-3C₆H₁₀O₅-C₈H₁₄O₂-H₂O]⁻,
Macrostemonoside F or Timosaponin C
Furostanol glycosides
[5, 27]

20
10.28
C₅₁H₈₄O₂₃
1063.5325
1063.5352
2.54
1063 [M-H]⁻,
273 [M-H-4C₆H₁₀O₅-C₈H₁₄O₂]⁻,
255 [M-H-4C₆H₁₀O₅-C₈H₁₄O₂-H₂O]⁻,
Timosaponin B IV (C51)
Furostanol glycosides
[5, 27]

21
10.60
C₄₅H₇₄O₁₈
901.4797
901.4824
3.00
739 [M-H-C₆H₁₀O₅]⁻,
577 [M-H-2C₆H₁₀O₅]⁻,
273 [M-H-3C₆H₁₀O₅-C₈H₁₄O₂]⁻,
255 [M-H-3C₆H₁₀O₅-C₈H₁₄O₂-H₂O]⁻,
Macrostemonoside F or Timosaponin C
Furostanol glycosides
[5, 27]

22
10.69
C₁₅H₁₂O₄
255.0658
255.0666
3.14
255 [M-H]⁻,
135 [M-H-C₇H₄O₂]⁻,
119 [M-H-C₇H₄O₃]⁻,
2′,4′,4-Trihydroxychalcone
Chalcone-type flavonoids
[29]

23
10.73
C₁₇H₁₆O₃
267.1021
267.1036
5.62
267 [M-H]⁻,
119 [M-H-C₉H₈O₂]⁻,
107 [M-H-C₁₀H₈O₂]⁻,
91 [M-H-C₁₀H₈O₃]⁻,
Oxy- hinokiresinol
Coumarins
[5]

24
11.00
C₁₆H₁₄O₅
285.0763
285.0777
4.91
285 [M-H]⁻,
165 [M-H-C₇H₄O₂]⁻,
119 [M-H-C₈H₆O₄]⁻,
(2S)-7,4′-Dihydroxy-5-methoxyflavone
Flavanones
[25]

25
11.23
C₁₆H₁₆O₅
287.0920
287.0931
3.83
287 [M-H]⁻,
269 [M-H-H₂O]⁻,
259 [M-H-CO]⁻,
2′-O-Methylphloretin
Chalcone-type flavonoids
[25]

26
11.38
C₃₉H₆₄O₁₄
755.4218
755.4218
0.00
755 [M-H]⁻,
593 [M-H-C₆H₁₀O₅]⁻,
575 [M-H-H₂O-C₆H₁₀O₅]⁻,
431 [M-H-2C₆H₁₀O₅]⁻,
271 [M-H-2C₆H₁₀O₅-C₈H₁₄O₂-H₂O]⁻,
253 [M-H-2C₆H₁₀O₅-C₈H₁₄O₂-2H₂O]⁻,
Timosaponin A II or Timosaponin G (C39) or Anemarrhena sapinin III
Spirostanol glycosides
[5, 27]

27
12.25
C₁₆H₁₈O₃
257.1178
257.1190
4.67
257 [M-H]⁻,
107 [M-H-C₉H₁₀O₂,
91 [M-H-C₉H₁₀O₃]⁻,
Broussonin A or Broussonin B
Coumarins
[29]

28
13.16
C₁₇H₁₆O₂
251.1072
251.1075
1.19
251 [M-H]⁻,
107 [M-H-C₁₀H₈O]⁻,
91 [M-H-C₁₀H₈O₂]⁻,
 Hinokiresinol
Coumarins
[29]

29
16.17
C₃₉H₆₄O₁₃
739.4269
739.4252
2.30
739 [M-H]⁻,
577 [M-H-C₆H₁₀O₅]⁻,
415 [M-H-2C₆H₁₀O₅]⁻,
273 [M-H-2C₆H₁₀O₅-C₈H₁₄O₂]⁻,
255 [M-H-2C₆H₁₀O₅-C₈H₁₄O₂-H₂O]⁻,
Timosaponin AIII
Spirostanol glycosides
[5, 27]

30
16.25
C₃₉H₆₄O₁₃
739.4269
739.4280
1.49
739 [M-H]⁻,
577 [M-H-C₆H₁₀O₅]⁻,
415 [M-H-2C₆H₁₀O₅]⁻,
273 [M-H-2C₆H₁₀O₅-C₈H₁₄O₂]⁻,
255 [M-H-2C₆H₁₀O₅-C₈H₁₄O₂-H₂O]⁻,
Timosaponin AIV
Spirostanol glycosides
[5, 27]

31
16.38
C₃₉H₆₄O₁₃
739.4269
739.4286
2.30
739 [M-H]⁻,
577 [M-H-C₆H₁₀O₅]⁻,
415 [M-H-2C₆H₁₀O₅]⁻,
273 [M-H-2C₆H₁₀O₅-C₈H₁₄O₂]⁻,
255 [M-H-2C₆H₁₀O₅-C₈H₁₄O₂-H₂O]⁻,
Smilageninoside
Spirostanol glycosides
[5, 27]

32
16.73
C₁₈H₁₈O₂
265.1229
265.1235
2.26
265 [M-H]⁻,
121 [M-H-C₁₀H₈O]⁻,
Monomethyl-cis-hinokiresinol
Coumarins
[29]

Figure 1: Base peak ion (BPI) chromatograms of substances in the extract of the Rhizoma Anemarrhenae herb, under negative ion mode.

3.2. Steroidal Saponins

Certain cleavage law exists under a certain MS condition for steroidal saponins. Steroidal saponins can be divided into two types: furostanol glycosides, which bear an open side chain at C-22, and spirostanol glycosides, which contain a closed spiroketal ring at C-22. Based on abundant fragment ions generated by losing sugar chains, side chains and dehydration steroidal saponins were divided into types I to V according to the numbers of hydroxyl group and double bond in the A, B, C, and D rings [23, 24, 27, 30].

First, [M-H-OH]⁻ or [M-H-OCH₃]⁻ molecular ion peaks were obtained as the CFs of furostan saponins, which display C22-OH or C22-OCH₃ on their structures, because furostan saponins preferentially lose the hydroxyl or methoxy on C-22. In contrast, spirostanol saponins generally produced [M-H]⁻ molecular ion peaks. In the negative ion mode, type I saponins produce the same fragment ions at m/z 273 [C₁₉H₂₉O]⁻, 255 [C₁₉H₂₇]⁻, 301 [C₂₁H₃₃O]⁻, and 283 [C₂₁H₃₁]⁻; types II to IV produce the same fragment ions at m/z 271 [C₁₉H₂₇O]⁻, 253 [C₁₉H₂₅]⁻, 299 [C₂₁H₃₁O]⁻, and 281 [C₂₁H₂₉]⁻; and type V saponins produce the same fragment ions at m/z 269 [C₁₉H₂₅O]⁻ and 251 [C₁₉H₂₃]⁻. Type IV saponins could be determined when fragment ions at m/z 271 [C₁₉H₂₇O]⁻, 253 [C₁₉H₂₅]⁻, 299 [C₂₁H₃₁O]⁻, and 281 [C₂₁H₂₉]⁻ are generated, whereas type II and type III saponins can be identified when fragment ions at m/z 289 [C₁₉H₂₉O₂]⁻ and 317 [C₂₁H₃₃O₂]⁻ appear in addition to the above-mentioned fragments. Fragment ions at m/z 271 [C₁₉H₂₇O]⁻ and 299 [C₂₁H₃₁O]⁻ resulted from the loss of sugar chains and side chains, followed by further loss of one water molecule for type II and type III saponins and the loss of the sugar chains and side chains only for type IV saponins. All steroidal saponins produce abundant characteristic fragment ions through the loss of the branched glycoside chains and side chains or by dehydration; the ions could be rapidly identified by combining CFs with NLs at m/z 162 (C₆H₁₀O₅), 132 (C₅H₈O₄), 142 (C₈H₁₄O₂) and 18 (H₂O). We used compounds purpureagitoside, timosaponin H1, and timosaponin AIII to discuss the fragmentation pathways in detail in the successive ESI–MSn experiments [5, 23, 24, 27, 30].

Compound 8 presented a retention time of 5.09 min and formula of C₅₆H₉₄O₂₉. Six fragment ions at m/z 1229 [M-H]⁻, 1211 [M-H-H₂O]⁻, 289 [M-H-H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻, 271 [M-H-2H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻, 253 [M-H-3H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻, and 281 [M-H-3H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₆H₁₀O₂]⁻ were detected in negative ion mode. The compound exhibit a molecular ion peak 1229 [M-H]⁻. The main fragment ions at m/z 289, 271, 253, and 281 were generated from the losses of sugar chain, along with the cleavage of the E-ring and dehydration. The fragment ion at m/z 289 displayed the sequential losses of 132 Da for a xyl molecule,  Da for four hexose molecules, and 142 Da (C₈H₁₄O₂) based on the fragment ion at m/z 1211 [M-H-H₂O]⁻. The fragment ion at m/z 271 [M-H-2H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻ was derived from the loss of one water molecule on the basis of the losses of sugar chains and side chains. According to NLs rules, Compound 8 can be inferred as furostanol glycosides. The fragment ion at m/z 1211 [M-H-H₂O]⁻ indicated the presence of hydroxyl at the position of C-22 due to the fact that furostanol glycosides preferentially lose hydroxyl located in C-22. Compound 8 can be identified as purpureagitoside through comparison with the literature [5, 27]. The fragmentation patterns of purpureagitoside are presented in Figure 2.

Figure 2: The fragmentation patterns of furostan saponins (type III), taking purpureagitoside as the example: the ion at m/z 1229 was the molecular ion [M-H]⁻, the fragmentation at m/z 1211 indicated a loss of 18 Da (H₂O) on the basis of the parent ion, the fragment ions m/z 431 displayed the loss of sugar group, the fragment ion (m/z 289) was formed by the cleavage of the E-ring on the basis of the fragment ion (m/z 431), and the fragment ions (m/z 271 and 253) were obtained by a loss of 18 Da (H₂O) or  Da (H₂O) on the basis of the fragment ion (m/z 289).

Compound 13, with a retention time of 8.12 min and a formula of C₅₆H₉₂O₂₈, exhibited fragment ions at m/z 1211 [M-H]⁻, 1193 [M-H-H₂O]⁻, 271 [M-H-H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻, and 253 [M-H-2H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻ in the MS spectrum. The compound produced molecular ions at m/z 1211 [M-H]⁻. The main fragment ions at m/z 271 [M-H-H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻ and 253 [M-H-2H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻ were both derived from the losses of sugar chain, 142 Da (C₈H₁₄O₂), and water molecule in the MS² spectrum, whereas the fragment ion of m/z 289 was not detected according to CFs and NLs rules shown in Table 1. Compound 13 was revealed as a furostanol glycoside (type IV). The fragment ion at m/z 1193 [M-H-H₂O]⁻ suggests that one hydroxyl group exists at the C-22 position of the compound because furostanol glycosides preferentially lose hydroxyl in C-22. The fragment ion at m/z 271 [M-H-H₂O-C₅H₈O₄-4C₆H₁₀O₅-C₈H₁₄O₂]⁻ was produced by the losses of sugar chains and side chains only. Thus, compound 13 was identified as timosaponin H1 [5, 27].

Compound 29 presented a retention time of 16.17 min and a formula of C₃₉H₆₄O₁₃. Several main fragment ions at m/z 739 [M-H]⁻, 577 [M-H-C₆H₁₀O₅]⁻, 415 [M-H-2C₆H₁₀O₅]⁻, 273 [M-H-2C₆H₁₀O₅-C₈H₁₄O₂]⁻, and 255 [M-H-2C₆H₁₀O₅-C₈H₁₄O₂-H₂O]⁻ were obtained in the mass spectrum. The compound was judged to be type I saponins according to the CFs of m/z 273 [M-H-2C₆H₁₀O₅-C₈H₁₄O₂]⁻ and 255 [M-H-2C₆H₁₀O₅-C₈H₁₄O₂-H₂O]⁻. The ion at m/z 577 [M-H-C₆H₁₀O₅]⁻ was produced by the loss of a hexose unit (Glc, 162 Da) from the molecular ion at m/z 739 [M-H]⁻. The fragment ion at m/z 415 [M-H-2C₆H₁₀O₅]⁻ could be explained by the ejection of a hexose unit (Gal, 162 Da) from the ion at m/z 577. The mass-to-charge ratio difference between the two fragment ions (m/z 415 and 273) was 142 Da, thereby suggesting that the fragment ion at m/z 273 was formed by the cleavage of the E-ring. Fragmentation at m/z 255 was obtained by the fragment ion m/z 273 to lose a molecule H₂O. According to CFs and NLs rules shown in Table 1, compound 29 was revealed as spirostanol saponins (type I). These results are consistent with the fragmentation pathway of timosaponin AIII [5, 27]. A proposed mechanistic pathway for fragments formed in MS is shown in Figure 3.

Figure 3: The fragmentation pathways of spirostanol saponins, taking purpureagitoside as the example: the ion at m/z 739 was the molecular ion [M-H]⁻, the ion at m/z 577 [M-H-C₆H₁₀O₅]⁻ was produced by the loss of a hexose unit (Glc, 162 Da), the fragment ion at m/z 415 [M-H-2C₆H₁₀O₅]⁻ could be explained by ejecting a pentose unit (Gal, 162 Da) from the ion at m/z 577, the fragment ion at m/z 273 was formed by the cleavage of the E-ring, and the fragmentation of m/z 255 was obtained by the fragmentation m/z 273 to lose a water molecule.

3.3. Flavonoids

Flavonoids were divided into mangiferin-type flavonoids, chalcone-type flavonoids, flavanones, homoisoflavonoids, and icariin-type flavonoids according to their chemical structures. Mangiferin-type flavonoids shared the same aglycone structure, the aglycone and sugar of which formed C-glycosides, and C-glycosides tended to lose CH₂O units during MS, and 2/3 and 3/4 CH₂O units could be eliminated from sugar molecules through 0,3X/0,2X cleavage [5, 25]; therefore, under the negative ion mode, the C-glycosides easily lost the neutral fragments at m/z 90 (C₃H₆O₃) and 120 (C₄H₈O₄), enabling the rapid and efficient identification of mangiferin-type flavonoids based on the two characteristic neutral loss ions. The structures of chalcone-type flavonoids possessed the same B ring and C ring cracking, with fragment ions at m/z 119 or 146 [C₉H₆O₂]⁻ produced by A and B ring cleavage during MS (negative ion mode). The compounds can be screened depending on these two characteristic fragment ions. Homoisoflavonoids exhibit the same B ring structure and reverse Diels-–Alder (RDA) of the C ring mainly occurred, producing fragment ion at m/z 133 [C₉H₉O]⁻, which could be recognized as the characteristic fragment ion of these compounds. Flavanones formed two main complementary fragments at m/z 119 [C₈H₇O] and 165 [C₈H₅O₄]⁻ by C ring for RDA cleavage. Anthocyanin aglycones possess the same aglycone structure and could be rapidly identified according to their aglycone fragment of m/z 367 [C₂₁H₁₉O₆]⁻ and neutral loss molecules of m/z 162 (C₆H₁₀O₅) and 132 (C₅H₈O₄) [5, 25, 29, 31, 32].

Compound 1 presented a retention time of 3.55 min and a formula of C₂₅H₂₈O₁₆, generating product ions at m/z 583 [M-H]⁻, 421 [M-H-C₆H₁₀O₅], 331 [M-H-C₆H₁₀O₅-C₃H₆O₃]⁻, and 301 [M-H-C₆H₁₀O₅-C₄H₈O₄]⁻. The fragment ion at m/z 583 [M-H]⁻ was a molecular ion. In addition, we obtained fragment ions at m/z 421 [M-H-C₆H₁₀O₅]⁻ that displayed a loss of 162 Da [C₆H₁₀O₅] based on the parent ion. We also obtained fragment ions at m/z 331 [M-H-C₆H₁₀O₅-C₃H₆O₃]⁻ and 301 [M-H-C₆H₁₀O₅-C₄H₈O₄]⁻, which corroborated with the rules of C-glycoside loss of 90 (C₃H₆O₃) and 120 Da (C₄H₈O₄) from sugar molecules through 0,3X/0,2X cleavage. These results follow the rules of CFs and NLs shown in Table 1. Compound 1 was rapidly and efficiently revealed as mangiferin-type flavonoids. Therefore, compound 1 was determined to be neomangiferin [5, 25]. The specific fragmentation process of neomangiferin is shown in Figure 4.

Figure 4: The specific fragmentation process of mangiferin flavonoids, take neomangiferin as an example: The fragment ion at m/z 583 [M-H]⁻ was a molecular ion, m/z 421 displayed a loss of 162 Da (C₆H₁₀O₅) based on the parent ion, the fragment ion at m/z 331 [M-H-C₆H₁₀O₅-C₃H₆O₃]⁻ conformed to the rule of C-glycosides loss 90 Da (C₃H₆O₃) and m/z 301 [M-H-C₆H₁₀O₅-C₄H₈O₄]⁻ followed the rules of C-glycosides loss 120 Da (C₄H₈O₄) from sugar molecules through 0,3X/0,2X cleavage.

Compound 22 possessed a retention time of 10.69 min and a formula of C₁₅H₁₂O₄. In the negative ion mode, several fragment ions at m/z 255 [M-H]⁻, 135 [M-H-C₇H₄O₂]⁻, and 119 [M-H-C₇H₄O₃]⁻ were detected; among the fragment ions, those at m/z 135 [M-H-C₇H₄O₂]⁻ and 119 [M-H-C₇H₄O₃]⁻ resulted from the cleavage by separation of A and B rings, and the fragment ion at m/z 119 [M-H-C₇H₄O₃]⁻ corresponded to the typical fragment of chalcone-type flavonoids according to CFs and NLs rules shown in Table 1. Besides, the fragment ion at m/z 255 [M-H]⁻ is a molecular ion. Compound 22 was rapidly revealed as chalcone flavonoids. Finally, compound 22 was identified as 2′,4′,4-trihydroxychalcone [29]. The specific fragmentation process of 2′,4′,4-trihydroxychalcone is shown in Figure 5.

Figure 5: The specific fragmentation process of chalcone flavonoids, take 2′,4′,4-trihydroxychalcone as an example: the fragment ion of m/z 255 [M-H]⁻ is molecular ion and the fragment ions at m/z 135 and 119 resulting from separation of A and B rings.

3.4. Phenylpropanoids

Phenylpropanoid compounds can be classified into lignans and coumarins according to different parent structures. Lignan compounds with the same A ring structure and benzyl groups usually produce the characteristic fragments of m/z 107 [C₇H₇O]⁻ and 91 [C₇H₇]⁻ by benzyl cleavage, as well as other fragment ions because of different linking groups to the A ring; then, the compound was determined according to the molecular ion fragments [29, 33].

Compound 27 presented a retention time of 12.25 min and a formula of C₁₆H₁₈O₃. In the negative ion mode, several fragment ions of m/z 257 [M-H]⁻, 107 [M-H-C₉H₁₀O₂]⁻, and 91 [M-H-C₉H₁₀O₃]⁻ were observed; among the fragments, the fragment ion at m/z 107 [M-H-C₉H₁₀O₂]⁻ was produced as a result of benzyl cleavage, and the fragment ion at m/z 91 [M-H-C₉H₁₀O₃]⁻ indicated a loss of water molecule from the fragment ion at m/z 107 [M-H-C₉H₁₀O₂]⁻. These two fragments show that this compound belongs to coumarin according to CFs rules shown in Table 1. Besides, the fragment ion of m/z 257 [M-H]⁻ as molecular ion was detected; thus, compound 27 was determined as Broussonin A or Broussonin B [29].

Compound 28 possessed a retention time of 13.16 min and a formula of C₁₇H₁₆O₂. In the negative ion mode, several fragments were observed at m/z 251 [M-H]⁻, 107 [M-H-C₁₀H₈O]⁻ and 91 [M-H-C₁₀H₈O₂]⁻ were observed. The fragment ion at m/z 107 [M-H-C₉H₁₀O₂]⁻ was produced as a result of benzyl cleavage. The fragment ions at m/z 91 [M-H-C₉H₁₀O₃]⁻ indicated a loss of water molecule based on the fragment ion at m/z 107 [M-H-C₉H₁₀O₂]⁻. Besides, the fragment ion at m/z 251 [M-H]⁻ as a molecular ion was detected. According to CFs rules shown in Table 1, compound 28 shared the same fragmentation pathways with compound 27 and belonged to coumarin. Therefore, compound 28 was determined as hinokiresinol [29, 33]. A pathway for fragments formed during MS is shown in Figure 6.

Figure 6: A fragmentation pathway of lignans formed in MS, take hinokiresinol as an example: the fragment ion of m/z 251 [M-H]⁻ was molecular ion and the fragment ion at m/z 107 was produced as result of benzyl cleavage. The fragment ions m/z indicated a loss of water molecular based on the fragment ion at m/z 107.

3.5. Benzophenones

Benzophenone compounds possess the same A ring structure, which easily loses 94 Da (A ring) and 44 Da (CO₂) in the negative ion mode. Therefore, benzophenone compounds in Rhizoma Anemarrhenae could be rapidly screened and identified according to the characteristics neutral loss of 94 Da and 44 Da in combination with molecular ion [26, 34].

Compound 12 presented a retention time of 7.49 min and a formula of C₁₄H₁₂O₅, thereby showing several fragments at m/z 259 [M-H]⁻, 165 [M-H-C₆H₆O]⁻, and 121 [M-H-C₆H₆O-CO₂]. The fragment ion at m/z 165 [M-H-C₆H₆O]⁻ indicates a loss of 94 Da (C₆H₆O); the difference between fragment ions (m/z 165 and 121) is exactly 44 Da, indicating a loss of one CO₂ molecule. The behavior follows the NLs rules shown in Table 1, and the compound was identified as benzophenone. Besides, m/z 259 [M-H]⁻ is the parent ion; thus, compound 12 was determined as 2,6,4′-trihydroxy-4-methoxybenzophenone [26, 34]. A pathway for fragments formed in MS is shown in Figure 7.

Figure 7: A fragmentation pathway of benzophenone formed in MS, take 2,6,4′-trihydroxy-4-methoxybenzophenone as an example: m/z 259 [M-H]⁻ is parent ion, the fragment ion at m/z 165 indicated a loss of 94 Da (C₆H₆O) based on the parent ion, and the fragment ion at m/z 121 indicated a loss of one CO₂ molecule based on the fragment at m/z 165.

3.6. Alkaloids

Alkaloids were divided into amide alkaloids with a N-C=O structure and pyridine alkaloids with N atoms in a six-member ring. In the negative ion mode, amide alkaloid with the same part of the structure easily loses a tyramine residue, resulting in neutral loss of molecule 119 (C₈H₇O). In addition, 15 Da (CH₃) can be easily lost based on the parent ion in the high mass-to-charge ratio. Given the same pyridine ring structure of pyridine alkaloids, the characteristic fragment ion at m/z 78 [C₅H₄N]⁻ was produced by removing the side chain to form the pyridine ring structure, and compounds were further identified through molecular ions [28, 35].

Compound 10 presented a retention time of 6.86 min and a formula of C₁₈H₁₉NO₄ and exhibited several fragments at m/z 312 [M-H]⁻, 297 [M-H-CH₃]⁻, and 178 [M-H-CH₃-C₈H₇O]⁻. The fragment ion at m/z 297 [M-CH₃]⁻ was observed in the high mass-to-charge ratio, displaying a loss of 15 Da (CH₃) based on the parent ion. In addition, the fragment ion at m/z 178 [M-H-CH₃-C₈H₇O]⁻ corroborated with the loss of 119 Da (C₈H₇O) based on the fragment ion at m/z 297. The behavior followed the CFs and NLs rules shown in Table 1, In addition, the fragment ion at m/z 312 [M-H]⁻ was the molecular ion. Thus, compound 10 was identified as N-trans-feruloyltyramine or N-cis-feruloyltyramine [28].

4. Conclusions

Based on the fragmentation patterns of five kinds of compounds in Rhizoma Anemarrhenae (as summarized from the literature), a combination of UPLC-Q-TOF/MS with data postprocessing techniques (CFF and NLF) was developed in this study to rapidly classify and identify 32 compounds in Rhizoma Anemarrhenae extracts. The results provide a basis for the pharmacological and pharmacokinetic studies on Rhizoma Anemarrhenae, overcome the shortage for traditional chemical analysis of TCM to a certain extent, and facilitate the advancement and development of TCM study.

Conflicts of Interest

The authors declare no conflicts of interest.

Authors’ Contributions

Yubo Li and Yanyan Xu conceived and designed the study. Lei Yuan, Lanlan Shan, and Yuanyuan Wu carried out the experiments. Lanlan Shan and Yani Zhang analyzed the research data and Lanlan Shan and Lei Yuan wrote the manuscript. All authors read and approved the final manuscript.

Acknowledgments

This project was supported by The National Natural Science foundation of China (no. 81573825, no. 81273998), Research Programs of Application of Basic and Frontier Technology in Tianjin (15JCYBJC29400), and Program for Changjiang Scholars and Innovative Research Team in University (IRT_14R41).

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