Antioxidant Potential of Herbal Preparations and Components from Galactites elegans (All.) Nyman ex Soldano

Galactites is a genus of flowering plants belonging to Asteraceae family. This genus is mainly represented by the Galactites elegans (All.) Nyman ex Soldano, the milky thistle, a plant of Mediterranean origin. Galactites elegans is consumed as a monofloral boar thistle honey. Chromatography separation of CHCl3 and n-BuOH extracts of aerial parts of G. elegans led to isolation of 18 pure compounds. Their structures were elucidated by 1D-and 2D-NMR spectroscopy and confirmed by mass spectrometry analysis. Sinapic aldehyde, abietin, chlorogenic acid, neochlorogenic acid, 8α-hydroxypinoresinol, 9α-hydroxypinoresinol, pinoresinol, 4-ketopinoresinol, nortrachelogenin, and erythro-guaiacylglycerol-β-O-4′-dihydroconiferyl alcohol were isolated from CHCl3 extract, while luteolin 4′-O-glucuronide, naringenin-7-O-neohesperidoside, kaempferol-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside, apigenin-7-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside, quercitrin, quercetin-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside, ciwujiatone, and nortrachelogenin-4,4′-di-O-β-D-glucopyranoside were obtained from n-BuOH extract. The majority of isolated compounds displayed a significant antioxidant potential in vitro test (DPPH). The ability of compounds to reduce the level of peroxides in control and BHP-treated Jurkat cells was studied. The lignan derivatives were also able to reduce at 50 μM the basal level of peroxides in Jurkat cells as well as counteract peroxide increase induced by BHP treatment. Particularly 8α-hydroxypinoresinol was the most active showing 70% of peroxide level inhibition.


Introduction
Galactites is a genus of flowering plants belonging to Asteraceae Compositae (commonly referred to as the aster, daisy, composite, or sunflower family) which is a very large and widespread family of flowering plants (Angiospermae). Many members belonging to this family are herbaceous, but a significant number are also shrubs, vines, or trees. The family has a worldwide distribution most commonly in the arid and semiarid regions of subtropical and lower temperate latitudes [1]. Asteraceae is an economically important family, providing products such as cooking oils, lettuce, sunflower seeds, artichokes, sweetening agents, coffee substitutes, and herbal teas. Plants in Asteraceae are medically important in areas that do not have access to Western medicine. They are also commonly featured in medical and phytochemical journals because the sesquiterpene lactone compounds contained within them are an important cause of allergic contact dermatitis [2].
This genus is mainly represented by the Galactites elegans (All.) Nyman ex Soldano, the milky thistle, a plant of Mediterranean origin (synonym: Galactites tomentosa Moench; common name: Scarlina). Galactites elegans is consumed as a monofloral boar thistle honey. This plant prefers sunny places 2 Evidence-Based Complementary and Alternative Medicine and usually grows on the uncultivated or barren grounds, waste places, well-drained soils, pastures, and roadsides [3][4][5].
In our systematic search for polyphenolic constituents from Algerian plants, we have investigated the aerial parts of Galactites elegans and report herein isolation and structural elucidation of 18 compounds and their antioxidant activities.

General Experimental Procedures.
Briefly optical rotations were measured on a Perkin-Elmer 241 polarimeter equipped with a sodium lamp (589 nm) and a 1 dm microcell. UV spectra were recorded on a Perkin-Elmer-Lambda spectrophotometer. NMR experiments were performed on a Bruker DRX-600 spectrometer at 300 K. HRES-IMS were acquired in positive ion mode on a Q-TOF premier spectrometer equipped with a nanoelectrospray ion source (Waters-Milford, MA, USA). Column chromatography was performed over Sephadex LH-20 (Amersham Biosciences; Uppsala, Sweden). Silica gel 60 (0.040-0.063 mm; Carlo Erba; Milan, Italy) was used as column material. HPLC separation was conducted on a Shimadzu LC-8A series pumping system equipped with a Shimadzu RID-10A refractive index detector and Shimadzu injector on a C 18 -Bondapak column (30 cm x 7.8 mm, 10 m Waters, flow rate 2.0 mL min −1 ). TLC was performed on precoated Kiesel gel 60 F 254 plates (Merck; Darmstadt, Germany); compounds were detected by Ce(SO 4 ) 2 /H 2 SO 4 (Sigma-Aldrich, Milan, Italy) solution; and reagent grade chemicals (Carlo Erba; Milan, Italy) were used throughout [6,7].

Extraction and Isolation.
Dried and powdered aerial parts of G. elegans (966 g) were macerated with MeOH-H 2 O (8:2) at room temperature. The operation repeated 3 times. The hydromethanolic extract was concentrated to dryness (under low pressure). The residue was suspended in H 2 O and successively partitioned with petroleum ether for 1 time then CHCl 3 , EtOAc, and n-BuOH (3 mL ×300 mL, each), respectively, affording a CHCl 3 soluble fraction (2 g), an EtOAc-soluble fraction (5.5 g), and a n-BuOH soluble fraction (19 g).
A part of CHCl 3 extract (1.87 g) was fractionated by column chromatography (CC) of Silica gel eluted with CHCl 3 followed by increasing concentrations of MeOH in CHCl 3 (between 1% and 100%), fractions were collected and monitored by TLC to obtain 20 fractions. Fraction 4 was chromatographed using RP18 HPLC with MeOH/H 2 O (2:3, v/v) as mobile phase (flow rate 2.0 mL min −1 ) to yield pure compounds ciwujiatone [16] (0.7 mg, t R 10 min), 4ketopinoresinol [17] (3) (1.9 mg, t R 40 min), pinoresinol [18] (1.2 mg, t R 48 min), and nortrachelogenin [19] (1) (2.1 mg, t R 57 min). Fraction [25] using gallic acid as standard, with some modifications [26]. The fraction solutions were mixed with 0.2 mL of 50% Folin-Ciocalteu reagent and allowed to react for 3 min and 1 mL aqueous solution of 2% Na 2 CO 3 was added. At the end of incubation for 45 min at room temperature, absorbance of each mixture was measured at 760 nm. The same procedure was also applied to the standard solutions of gallic acid. Total phenolic contents were expressed as g gallic acid equivalents per mg of the fractions.

DPPH Radical Scavenging Assay.
Radical scavenging activity was determined by a spectrophotometric method based on the reduction of a methanol solution of DPPH using the method of Blois [27]. The sample solutions were added to 0.004% methanol solution of DPPH. The mixture was shaken vigorously and left to stand at room temperature for 30 min in the dark. The absorbance was measured at 517 nm against a blank by a spectrophotometer (Rayleigh, UV-2601). Scavenging of DPPH radical was calculated according to formula: (1) where control is the absorbance of the control reaction (containing all reagents except the test compound) and sample is the absorbance of the test compound. DPPH scavenging activity was expressed as IC 50 values ( g/mL) for comparison. The IC 50 value of each sample was defined as the concentration of sample required for a 50% decrease in absorbance of the blank. BHT and BHA were used as positive controls.

Metal Chelating Activity on Ferrous Ions (Fe 2+ ).
Metal chelating activity was determined according to the method of Decker and Welch [28], with some modifications [29,30]. Briefly, 0.5 mL of the samples was mixed with 0.05 mL 2 mM FeCl 2 and 0.1 mL 5 mM ferrozine. The mixture was diluted with methanol (2 mL) and left standing at room temperature for 10 minutes. The absorbance of the solution was measured spectrophotometrically at 562 nm. EDTA was used as a positive control.

Cell Cultures.
Jurkat cells (a T-cell leukemia cell line obtained from Cell Bank in GMP-IST, Genova, Italy) were maintained in RPMI 1640 medium supplemented with 10% (v/v) FBS, 2 mM L-glutamine, and antibiotics at 37 ∘ C in humidified atmosphere with 5% CO 2 . To ensure logarithmic growth, cells were subcultured every three days. Stock solutions (50 mM) of polyphenolic compounds in DMSO were stored at -20 ∘ C and appropriately diluted in the same solvent or directly in the medium just before use (DMSO never exceeding 0,5%).

Peroxide Depletion Activity of Test Compounds by
Cytofluorometry. The evaluation of intracellular peroxides concentration was performed according to Rothe [30] with some modifications. In detail, Jurkat cells were collected by centrifugation and suspended in RPMI containing 5% FBS at a density of 5 x 10 5 cells/mL. FBS concentration was lowered to 2% to increase the uptake rate of flavonoids and reagents in the short-incubation time (1 hr) chosen for the assay. Cell suspensions were incubated with increasing concentrations of each chemical or vehicle only at 37 ∘ C. For each sample duplicate test tubes were prepared. After 30 min of incubation, BHP (550 M final concentration) or an equal volume of vehicle was added. This allowed us to monitor the effect of flavonoids on BHP-induced peroxide elevation or the basal levels of peroxides, respectively. In the last 15 min of incubation cells were loaded with DCFH-DA (8 M final concentration). Test tubes were gentle mixed several times along the incubation period (1 hr). Cells suspension were then washed and resuspended in an equal volume of medium and 10,000 events were analyzed for DCF-fluorescence by cytofluorometry (BD FACSCalibur6 instrument, Becton Dickinson, San Jose, CA, USA). DCF green fluorescence was analyzed in the FL1 channel ( exc 488 nm; ( em 535 nm). Before the cytofluorometric analysis an aliquot of each sample was withdrawn to evaluate cell viability by Trypan-blue exclusion test.

Statistical Analysis.
All experiments were performed in triplicate and the results were expressed as mean ± SD. Statistical analyses were performed using the SPSS 11.5 (SPSS, Chicago, IL). For DPPH activity, differences among means were done by analysis of variance (ANOVA), and averages were compared using the Duncan test. For other tests, differences between treatment groups were analyzed by the student test. Differences were considered significant when P <0.05.

Antioxidant Activities of Extracts and Components from Galactites elegans
3.1.1. Total Phenolic Content. Phenolic compounds are characterized by having at least one aromatic ring with one or more hydroxyl groups attached which directly contribute to the antioxidant properties [31]. Therefore, it is important to evaluate the total phenolic in the extracts from Galactites elegans. The contents of total phenolic compounds in the   extracts, expressed as g gallic acid equivalents per milligram of dry extract, are shown in Table 1.

DPPH Radical Scavenging
Activity. DPPH radical scavenging activities of the extracts and the reference synthetic agents are given in Table 1. According to these IC 50 values, the DPPH radical scavenging abilities among the different extracts were in the order of CHCl 3 > n-BuOH (P < 0.05). Furthermore, pure compounds radical scavenging abilities is reported in Table 2. Lower IC 50 value indicates higher free radical scavenging activity.

Metal Chelating Activity.
Iron ions catalyse the conversion of less reactive species such as H 2 O 2 or lipid peroxides into more reactive ones such as hydroxyl or peroxyl/alkoxyl radicals. Therefore, extracts with iron chelating ability can act as powerful antioxidants [32]. The metal chelating ability of the extracts was investigated by ferrozine assay. The chelating potential of n-BuOH extract was determined as 38.5 ± 1.4 % that was significantly lower (p< 0.01) than synthetic chelating agent EDTA (93.7 ± 0.3 %) at the concentration of 2 mg/mL. On the other hand, chloroformic extract was not effective at the tested concentration.

Antioxidant Potential of Test Compounds by Cytofluorometry.
This study was carried out in order to discover natural compounds which could be used as active ingredients to improve the health and/or physical appearance of the skin or as preservatives or stabilizers for other active ingredients or vehicles in topical formulations. As reported in a recent work of Korte [33], natural products which show this kind of bioactivity are lignans and lignan esters. For these reasons, among all the tested molecules, pinoresinol and the northrachelogenin derivatives have been selected (Figure 1; Particularly, the in cell antioxidant potential of nortrachelogenin (1), nortrachelogenin-4,4 -di-O--Dglucopyranoside (2), and 8 -hydroxypinoresinol (4) have been investigated. 4-ketopinoresinol (3) was used as positive control as reported in Chen [17], however pinoresinol was not tested because of its high cytotoxicity, instead 9 hydropinoresinol was not active [21]. The ability of test compounds to reduce the level of peroxides in control and BHP-treated Jurkat cells was measured by cytofluorometry. Each chemical was tested at 25 M and 50 M concentrations, being the latter the maximum dose not cytotoxic. They were able to reduce the basal level of peroxides in Jurkat cells as well as counteract peroxide increase induced by BHP treatment. The slight lower antioxidant potential of nortrachelogenin 4,4 -di-O-ß-D-glucopyranoside (2) could be ascribed to the presence of a carbohydrate moiety which, if on one hand could slight contribute to the antioxidant activity and on the other hand might reduce nortrachelogenin 4,4di-O-ß-D-glucopyranoside availability for Jurkat cells. In addition,-the sugar component seems to be responsible for the higher cytotoxic potential of nortrachelogenin 4,4 -di-Oß-D-glucopyranoside, possibly due to perturbation of plasma membrane (Figure 2).

Conclusion
All the isolates (except chlorogenic acid) were first reported from the genus Galactites and the majority of isolated phenolic components displayed a significant antioxidant potential in vitro assay (DPPH). The lignan derivatives were also able to reduce at 50 M the basal level of peroxides in Jurkat cells as well as counteract peroxide increase induced by BHP treatment.
Particularly  This result suggests that active fractions could be used as a source of antioxidant agent for pharmaceutical and cosmetic preparations.