The effects of XCHD on the proliferation of C6 cells and on factors associated with the microRNA-34a (miR-34a)/p53/caspase-3 signaling pathway in vitro were investigated. Methods. XCHD was purchased too much to complete the study. CCK-8 assay was used to measure the XCHD concentration, and qPCR was used to quantify miR-34a expression at the mRNA level. Apoptosis was assessed using TUNEL. Western blots were used to determine the p53, caspase-3, caspase-8, and Bcl-2 expression levels. Results. The optimal XCHD concentration and time effect for C6 cells were observed after 36 h of exposure to a concentration of 100 µg/ml XCHD. miR-34a expression increased 8 and 12 h after the addition of XCHD. The presence of XCHD decreased Bcl-2 expression but increased p53, cleaved caspase-3, Bax, and caspase-8 expression. When p53 was inhibited, miR-34a expression was unaffected by the addition of XCHD, Bcl-2 expression was low, and cleaved caspase-3, Bax, and caspase-8 expression increased. The inhibition of p53 promoted C6 cell growth when compared with C6 cells exposed to XCHD and with no inhibition of p53. Conclusions. XCHD inhibits C6 cell growth which was influenced by the p53/caspase pathway.
Inner Mongolia UniversityYKD2016KJBW023Natural Science Foundation of Inner Mongolia2019MS08153Inner Mongolia Medical Universitybsjj2016161. Introduction
Glioma cells are the most common type of intracranial primary tumor cells, and they exhibit invasive growth and possess a high postoperative recurrence [1, 2]. Glioblastomas pose a serious threat to the lives and health of patients. Many patients do not recover from this type of cancer even after undergoing operations, radiotherapy, and chemotherapy [3]; their median survival time is approximately 14.6 months [4]. Treating glioblastomas is difficult because a very small fraction of small cell subsets (1%) cannot be killed after being treated with radiotherapy and chemotherapy [5–7]. Therefore, the investigation of novel potential therapeutic agents, such as those used in traditional Chinese medicine (TCM), and the molecular mechanisms underlying their cytotoxic effect via clinical investigations is necessary [3, 4, 8].
TCM is widely used in China and is becoming increasingly prevalent in Europe and America. Several complex formulas and crude materials used in Chinese medicines, including Xiaochaihu decoction (XCHD), have demonstrated measurable activity against viral hepatitis in the central nervous system [9–11]. XCHD is a classic formulation, which is described in a “Treatise on Cold Pathogenic and Miscellaneous Diseases” as having anti-inflammatory effects and providing gastric protection and regulation of the immune system [12–14]. XCHD reduces the number of preneoplastic cells, inhibiting the development of liver tumors [15–17]. However, few studies have focused on the efficacy of XCHD in terms of its effects on primary tumors of the central nervous system such as glioma.
The cellular response to gliomas has been widely studied; however, the role of microRNA (miRNA/miR) in gliomas remains largely unknown [18, 19]. The functions of many miRNAs are associated with gliomas [20]. Because of the numerous functions of miRNA, studies involving the miRNA profile associated with gliomas are necessary [21].
p53, a transcription factor that stops the cell cycle and induces a proapoptotic effect via the modulation of multiple target genes, is another well-known tumor suppressor in cancer research [22, 23]. In murine brains, p53 deficiency plays a central role in driving gliomagenesis [24]. Moreover, p53 deficiencies contribute to neuronal death after transient cerebral ischemic injury, while delayed treatment with p53 inhibitor could facilitate endogenous neurogenesis and therefore improve functional recovery [25]. Several p53-regulated miRNAs have been implicated, including the miR-17-92 cluster, miR-145, and let-7 [23]. Importantly, p53 regulates tumor cell recognition via the p53-regulated miRNA miR-34a [23].
To elucidate the possible molecular mechanism of using XCHD to treat glioma, a serum pharmacological method was used in the present study. The effects of XCHD on the proliferation of C6 cells and on factors associated with the miR-34a/p53/caspase-3 signaling pathway in vitro were investigated.
2. Materials and Methods
XCHD was purchased from the Chinese National Institute (Beijing, China). A decoction of XCHD (chaihu, huangqin, renshen, gancao, banxia, shengjiang, and dazao) was prepared from a mixture of chopped crude herbs, extracted in distilled water at 100°C; this mixture was then concentrated to a final concentration of 100 crude drug gram per 100 milliliters. And primary antibodies were purchased from Abcam. miRNA expression assay kits were purchased from Tiangen, and p53 inhibitor pifithrin-α was purchased from Sigma.
2.1. Cell Culture
C6 glioma cells (Chinese Academy of Sciences, China) were cultured at 37°C in a humidified atmosphere containing 5% CO2.
2.2. Cell Proliferation Assay
A Cell Counting Kit-8 (CCK-8) assay was used to measure cell viability and proliferation. Cells were seeded in 96-well plates with different concentrations of XCHD (0, 10, 50, 100, and 200 µg/ml) and were incubated for different periods of time (12, 24, 36, and 48 h).
2.3. Western Blotting
Cell protein samples were separated via 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes. The membranes were blocked in 5% nonfat milk diluted with Tris-buffered saline/Tween 20 (TBST) at room temperature for 1 h and incubated overnight at 4°C with primary antibodies. The membranes were then washed three times and developed using enhanced chemiluminescence (ECL) reagents (Pierce, Rockford, USA).
2.4. Flow Cytometric Analysis
Cells (1 × 106) were washed with phosphate-buffered saline (PBS) and then labeled using Annexin V and propidium iodide (PI). Apoptotic rates were measured using flow cytometry and FlowJo software. The percentage of apoptotic cells was measured by applying the criteria of Annexin V positivity and PI negativity, while the percentage of late apoptotic cells was determined by applying the criteria of Annexin V positivity and PI positivity.
Total cellular RNA was obtained using an RNA extraction kit, and complementary DNA (cDNA) was synthesized from 1 μg of total RNA. RT primers and a TaqMan miRNA assay kit (Applied Biosystems, USA) were used to perform reverse transcription and PCR according to the manufacturer’s instructions.
2.6. Statistical Analysis
One-way analysis of variance (ANOVA) was performed using GraphPad Prism version 5 software. The data are presented as the mean ± standard deviation (SD) P values less than 0.05 were considered significant.
3. Results3.1. Effects of XCHD on C6 Glioma Cell Growth and XCHD Concentration
A 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay was used to assess cell growth and viability following treatment of C6 cells with different concentrations of XCHD and determine the appropriate treatment duration to achieve the desired level of cellular growth.
XCHD was added to the growth medium to achieve final XCHD concentrations of 0, 10, 50, 100, and 200 µg/ml. The growth percentages obtained for each XCHD concentration were assessed at 12, 24, 36, and 48 h (Figure 1(a)). The results showed the optimal concentration and XCHD treatment duration for the attenuated growth of C6 cells were 100 µg/ml XCHD and 36 h, respectively.
XCHD effect on the growth of C6 cells. (a) Growth percentage of different concentrations (0, 10 μg/ml, 50 μg/ml, 100 μg/ml, and 200 μg/ml) XCHD effected on the C6 cells at 12 h, 24 h, 36 h, and 48 h. (b) TUNEL detected the C6 cell apoptosis status effected by XCHD with 100 μg/ml at 12 h, 24 h, 36 h, and 48 h. A: 12 h, B: 24 h, C: 36 h, and D: 48 h.
To further examine the effects of XCHD on C6 cells, terminal deoxyribonucleotidyl transferase-mediated biotin-16-dUTP nick-end labeling (TUNEL) was used to study the effects of 100 µg/ml XCHD on C6 cells at 12, 24, 36, and 48 h. The results (Figure 1(b)) showed the cell concentrations at 36 and 48 h were very low.
3.2. Effects of XCHD on miR-34a Expression in C6 Cells
Quantitative PCR (qPCR) was used to determine miR-34a expression at the mRNA level after the addition of XCHD. The results (Figure 2) showed miR-34a expression levels were higher at 8 and 12 h than those at 0 and 4 h (P<0.05). Furthermore, at 4 h, the miR-34a expression level was higher than that at 0 h (P<0.05).
Relative miR-34a expression levels effected by XCHD. ∗p<0.05 versus 0. #p<0.05 versus 4 h.
3.3. XCHD Attenuated C6 Growth via the p53/Caspase Pathway
To determine the signaling pathways mediating the attenuating effect of XCHD on C6 cell growth, the p53, cleaved caspase-3, caspase-8, Bcl-2, and Bax protein levels were quantified after 0, 4, 8, and 12 h (Figure 3(a)) of exposure to 100 µg/ml XCHD. XCHD exposure decreased the Bcl-2 protein level; however, the p53, cleaved caspase-3, caspase-8, and Bax protein levels increased after XCHD exposure (Figure 3(b)). These results showed p53/caspase signaling plays a key role in the inhibitory effect of XCHD on C6 cell growth. To verify these results, p53 inhibitor was used to silence p53 expression in addition to XCHD. Bcl-2 expression was low during the entire experimental period in C6 cells exposed to both the p53 inhibitor (120 nM) and XCHD, but the Blc-2 expression level was higher than that of the conditions that did not use p53 inhibitor. In addition, the cleaved caspase-3, caspase-8, and Bax protein levels were higher than those obtained with XCHD alone (Figure 4). miR-34a expression was lower in C6 cells exposed to both XCHD and the inhibitor of p53 expression (Figure 5). The inhibition of p53 expression promoted C6 growth when compared with the effect of XCHD exposure only (Figure 6).
p53, cleaved caspase-3, caspase-8, Bcl-2, and Bax protein expression effected by XCHD. (a) The expression of p53, cleaved caspase-3, caspase-8, Bcl-2, and Bax proteins. (b) Relative protein expression (protein/GAPDH) of p53, cleaved caspase-3, caspase-8, Bcl-2, and Bax based on the western blot.
Influence of C6 cell after p53 inhibition. (a) The expression of p53, cleaved caspase-3, caspase-8, Bcl-2, and Bax proteins. (b) Relative protein expression (protein/GAPDH) of p53, cleaved caspase-3, caspase-8, Bcl-2, and Bax based on the western blot.
Relative miR-34a expression levels. Influence of C6 cell after p53 inhibition. ∗p<0.05 versus 0. #p<0.05 versus 4 h.
Growth percentage effected on the C6 at 12 h, 24 h, 36 h, and 48 h.
4. Discussion
Brain glioma tumors are often lethal to humans [26, 27]. Numerous studies have demonstrated XCHD and its active components exhibit notable therapeutic effects in cancer cells when using XCHD as a TCM [28, 29]. In this study, XCHD inhibited the growth of C6 cells via the p53/caspase pathway.
XCHD, derived from a classic TCM described in the “Treatise on Febrile Diseases,” has exhibited positive effects in the treatment of chronic hepatitis B (CHB) in China and is a classic treatment method for promoting bile flow [30]. XCHD can be anticancer [28]. The results of this study showed XCHD inhibits the growth of C6 cells.
XCHD is composed of Chinese thorowax root, Scutellaria baicalensis, Pinellia ternata, ginger, ginseng, Ziziphi jujubae, liquorice root, Phyllanthus urinaria, largehead atractylodes rhizome, Poria cocos, white peony root, and Fructus schisandrae. XCHD inhibits the growth of liver cancer cells via the JAK2/STAT3 pathway [31]. The results of this study demonstrated XCHD attenuated the growth of C6 cells via activation of the miR-34a/p53 pathway. The addition of XCHD promoted the expression of p53 and increased miR-34a. In addition, miR-34a expression levels were correlated with the rate of apoptosis in C6 cells, in which tumor suppressor p53 activated miR-34a and downregulated Bcl-2. Following miR-34a/p53 expression, cleaved caspase-3 and caspase-8 were activated and apoptosis was induced via the mitochondrial pathway.
XCHD inhibits C6 cell growth which was influenced by the p53/caspase pathway. Thus, the results of this study suggest XCHD could be useful in the inhibition of C6 cells and protecting the glioma.
Data Availability
The data are presented as mean ± standard deviation (SD); each data was repeated five times.
Conflicts of Interest
The authors declare no conflicts of interest.
Acknowledgments
This work was supported by the Inner Mongolia University Kejibaiwan Project (no. YKD2016KJBW023), Inner Mongolia Natural Science Foundation (no. 2019MS08153), and Inner Mongolia Medical University Doctor Project (no. bsjj201616).
AnY.ZhangD.-D.YuH.-L.27-Hydroxycholesterol regulates cholesterol synthesis and transport in C6 glioma cells201759889710.1016/j.neuro.2017.02.0012-s2.0-85016272993BernierM.PaulR. K.DossouK. S. S.Antitumor activity of (R,R′)-4-methoxy-1-naphthylfenoterol in a rat C6 glioma xenograft model in the mouse201312e0001010.1002/prp2.102-s2.0-84979949855ChaoC.-C.KanD.LuK.-S.ChienC.-L.The role of microRNA-30c in the self-renewal and differentiation of C6 glioma cells201514221122310.1016/j.scr.2015.01.0082-s2.0-84923012090ChenY.HuangH.YaoC.Antitumor activity of combined endostatin and thymidine kinase gene therapy in C6 glioma models2016592477248610.1002/cam4.7982-s2.0-84991086733ChangK.-W.HuangY.-L.WongZ.-R.Fibroblast growth factor-2 up-regulates the expression of nestin through the Ras-Raf-ERK-Sp1 signaling axis in C6 glioma cells2013434485486010.1016/j.bbrc.2013.04.0312-s2.0-84878182613ChenY.-D.ZhangY.DongT.-X.Hyperthermia with different temperatures inhibits proliferation and promotes apoptosis through the EGFR/STAT3 pathway in C6 rat glioma cells20171669401940810.3892/mmr.2017.77692-s2.0-85032735472DongX.-z.HuangC.-l.YuB.-y.HuY.MuL.-h.LiuP.Effect of Tenuifoliside A isolated from Polygala tenuifolia on the ERK and PI3K pathways in C6 glioma cells201421101178118810.1016/j.phymed.2014.04.0222-s2.0-84904977189BoultJ. K.TerkelsenJ.Walker-SamuelS.BradleyD. P.RobinsonS. P.A multi-parametric imaging investigation of the response of C6 glioma xenografts to MLN0518 (tandutinib) treatment201384e6302410.1371/journal.pone.00630242-s2.0-84876880478GuB. Q.YangJ. M.ZhaoY. Y.A preliminary observation on effects of xiaochaihu decoction on uropathogenic E. coli19971710599600QinX.Xiaochaihu Tang for treatment of chronic hepatitis B: a systematic review of randomized trials20108431232010.3736/jcim201004032-s2.0-77950901514ZhangS. K.CuiN.-q.ZhuoY.-z.Modified Xiaochaihu decoction promotes collagen degradation and inhibits pancreatic fibrosis in chronic pancreatitis rats201726859960310.1007/s11655-017-2413-02-s2.0-85035090368DaiJ.-y.YangJ.-l.LiC.Transport and metabolism of flavonoids from Chinese herbal remedy Xiaochaihu-tang across human intestinal Caco-2 cell monolayers120082991086109310.1111/j.1745-7254.2008.00850.x2-s2.0-51349086819JiaoL.QiX.LuG.ZhangQ.ZhangC.GaoJ.Effect of traditional Chinese medicine (Xiaochaihu Tang) on the expression of MMP-2 and MMP-9 in rats with endometriosis2013661385138910.3892/etm.2013.13162-s2.0-84888417210LiuX. Q.HuX.-j.XuH.-X.ZengX.-Y.Xiaochaihu decoction attenuates the vicious circle between the oxidative stress and the ALP inactivation through LPS-catecholamines interactions in gut, liver and brain during CCI4+ ethanol-induced mouse HCC201313137510.1186/1472-6882-13-3752-s2.0-84891023021ChenP.LiC.LiangS.Characterization and quantification of eight water-soluble constituents in tubers of Pinellia ternata and in tea granules from the Chinese multiherb remedy Xiaochaihu-tang2006843218319310.1016/j.jchromb.2006.05.0282-s2.0-33751044187LiJ.XieM.GanY.Effect of Xiaochaihu decoction and different herbal formulation of component on inhibiting H22 liver cancer in mice and enhancing immune function200833910391044QingchunL.Simultaneous determination of five active constitutents in Xiaochaihu Tang by HPLC201035670871010.4268/cjcmm201006102-s2.0-77953498531ChenG.ZhouT.LiY.YuZ.SunL.p53 target miR-29c-3p suppresses colon cancer cell invasion and migration through inhibition of PHLDB220174871909510.1016/j.bbrc.2017.04.0232-s2.0-85017242292ChenJ.ZhaoK. N.HPV-p53-miR-34a axis in HPV-associated cancers2015321331CaoC.ZhangH.ZhaoL.miR-125b targets DNMT3b and mediates p53 DNA methylation involving in the vascular smooth muscle cells proliferation induced by homocysteine201634719510410.1016/j.yexcr.2016.07.0072-s2.0-84995639933CaiZ.CaoR.ZhangK.Oncogenic miR-17/20a forms a positive feed-forward loop with the p53 kinase DAPK3 to promote tumorigenesis201529032199671997510.1074/jbc.m115.6615042-s2.0-84939183228ChenL.LuoL.ChenW.MicroRNA-24 increases hepatocellular carcinoma cell metastasis and invasion by targeting p53: miR-24 targeted p532016841113111810.1016/j.biopha.2016.10.0512-s2.0-84993928881CortezM. A.IvanC.ValdecanasD.PDL1 Regulation by p53 via miR-342016108110.1093/jnci/djv3032-s2.0-84956573071ForoutanT.FarhadiA.SoltaniB. M.Adipose derived stem cells affect miR-145 and p53 expressions of co-cultured hematopoietic stem cells2018194654659LiJ.AungL. H. H.LongB.miR-23a binds to p53 and enhances its association with miR-128 promoter2015511642210.1038/srep164222-s2.0-84946708122CapocciaE.CirilloC.MarchettoA.S100B-p53 disengagement by pentamidine promotes apoptosis and inhibits cellular migration via aquaporin-4 and metalloproteinase-2 inhibition in C6 glioma cells2015962864287010.3892/ol.2015.30912-s2.0-84929996128ChenY.YangS.-H.HuengD.-Y.SyuJ.-P.LiaoC.-C.WuY.-C.Cordycepin induces apoptosis of C6 glioma cells through the adenosine 2A receptor-p53-caspase-7-PARP pathway2014216172510.1016/j.cbi.2014.03.0102-s2.0-84898983728YanL.Study on transportation of xiaochaihu tang in caco-2 cell model201136810871090ZhuZ.ZhaoL.LiuX.Comparative pharmacokinetics of baicalin and wogonoside by liquid chromatography-mass spectrometry after oral administration of Xiaochaihu Tang and Radix scutellariae extract to rats2010878242184219010.1016/j.jchromb.2010.06.0212-s2.0-77955423999LiS. M.XuS. J.HongW. X.Study on clinical experience of using xiaochaihu decoction2014341012641266ChenS.WangZ.WanS.HuangH.LiangH.Effect of modified Xiaochaihu decoction-containing serum on HepG2.2.15 cells via the JAK2/STAT3 signaling pathway20171657416742210.3892/mmr.2017.75612-s2.0-85030116883