Ethnopharmacological Study of Medicinal Plants in Bajwat Wildlife Sanctuary, District Sialkot, Punjab Province of Pakistan

Bajwat Wildlife Sanctuary is a complex riverine ecosystem and is unique because of the presence of river Chenab, various seasonal streams, lakes, and Head Marala barrage. These ecogeographic conditions provide diverse natural habitats for various plant and animal species to grow uninterrupted and have undocumented ethnopharmacologically important medicinal flora. The present study involves the first-ever extensive investigation to document the ethnopharmacological knowledge on medicinal plants of local healers and inhabitants of the Bajwat Wildlife Sanctuary to treat ailments. The unstructured and semistructured interviews of the local healers and inhabitants were conducted that included 130 individuals. The ethnomedicinal formulations, their method of preparation, mode of administration, parts of the plant used, diseases cured, and their categorization along with species use report (UR) were analyzed. The ethnopharmacological study led to the enlisting of 114 medicinal plant species belonging to 97 genera and distributed among 47 plant families. 2029 URs were collected with 42 general disease categories. Each plant species was reported 18 times to cure various diseases (∼18 UR), while ∼48 URs were collected on each disease category by local informants. Digestive issues (290 URs, ∼14.29%) and skin infections (279 URs, ∼13.75%) were found most commonly among the occupants of the area. The oral administration (69%) of herbal drugs and the preparation of plant extracts (32%) were the most common ethnopharmacological strategies. Inhabitants of the area were well aware of the limited use of poisonous plants. 8 (∼7%) out of the total 114 medicinal plant species were listed in the IUCN Red List of Threatened Species as Least Concern, while Eucalyptus camaldulensis Dehnh. was enlisted as near-threatened. The results of the present investigation show that the occupants of the Bajwat have sound information about the ethnopharmacological consumption of medicinal plants, and some of the novel ethnomedicinal formulations were reported which provide the basic data for further pharmacological research.


Introduction
e traditional herbal medicine system in Pakistan is originated from Ayurveda medicine which has a history of 2500-600 BC [1]. e first documented record of the use of plant medicine in the subcontinent (Indo-Pak) is available in Rigveda (Rigveda is the oldest religious book of the Hindu religion. Many herbal remedies are documented in this book to treat ailments.) and dates to 4500-1600 BC. e herbal medicine system in Pakistan is greatly influenced by the traditional Chinese medicine system, Persian medicines, Greek medicine system [2], and prescriptions from the Prophet Muhammad (PBUH). e Tib-Al-Nabwi or Prophetic Medicine system has 1500-year-old history [3,4]. ese diverse features of the traditional healthcare system of Pakistan embellish it with great potential to use plants to treat various ailments. e tertiary healthcare system in Pakistan is responsible to provide basic to advanced healthcare facilities to the inhabitants. e rural areas in Pakistan have limited healthcare services. Rural healthcare communities have facilities in the form of Rural Health Centers (RHCs) and Basic Health Units (BHUs) that contribute to cover a population of 25,000 inhabitants and are responsible to provide primary healthcare and referral services [5,6]. Most of the rural communities of Pakistan use herbal medicines because of the limited access to primary healthcare services, expensive medicines, and diagnostic tests [7]. Pakistan is blessed with a great number of higher plants (5,700). Out of the enlisted higher plants, 600-700 are enumerated as therapeutic plants utilized by around registered herbalists and homeopaths. e pharmacopeia endorsed by the National Council of Tibb (Herbalist committee) enlisted 900 medicinal plants commonly used in Pakistan to prepare herbal medicines [8,9].
Bajwat is situated in the district Sialkot (32°62 N and 74°60 E) and on the outskirts of the district Narowal region of Punjab territory in Pakistan ( Figure 1). It contains 84 towns or small villages, with a population of 60,000 as indicated by the 2017 survey. e territory is a wetland of universal significance because of the nearness of Marala Headworks (barrage), Munawar Tawi (river), Jammu Tawi (river), and numerous natural streams, lakes, and muddy regions. Bajwat is situated in a transitional zone between the slopes of Jammu in Indian-occupied Kashmir and the plain zone of Punjab. Bajwat Wildlife Sanctuary has an absolute region of 5,464 hectares which provides free space to wildlife flora and fauna [10]. e most common and universally rare vertebrates of the area include the wild pig or wild boar (Sus scrofa L.), deer (Odocoileus virginianus Zimmermann), and nilgai (Boselaphus tragocamelus Pallas) (blue bull, largest Asian antelope) [11]. ese special ecogeographic attributes and the presence of important plant and animal species and serious threats to them contributed to getting the special status for the area [12]. e vast floristic wealth and the rich knowledge of the inhabitants of Bajwat require necessary steps to document this traditional ethnopharmacological knowledge which can help forest officials, NGOs, policymakers, local healers, and researchers that are involved in ethnopharmacological research on medicinal plants. ere are very few ethnobotanical studies on medicinal plants reported previously from the district Sialkot [13][14][15][16][17]. ese studies were performed superficially, do not provide detailed ethnomedicinal knowledge of the district Sialkot, and do not cover Bajwat Wildlife Sanctuary. e importance of the ethnopharmacological consumption of the medicinal plants of Bajwat Wildlife Sanctuary was ignored, and there was no study to document the significance of the conserved flora.
It was hypothesized that the long-term and well-driven ethnopharmacological survey on medicinal plants of Bajwat Wildlife Sanctuary may help us to enlist ethnopharmacologically important medicinal plants of the area. It can be the first-ever study to document the ethnopharmacological knowledge of the local inhabitants and healers to use plants to treat diseases.

Aim of the Study.
e present study was aimed to significantly establish the first-ever inventory of therapeutically important medicinal plants of the Bajwat Wildlife Sanctuary, which is endowed with unique biodiversity and gained a special status to conserve the local flora and fauna. e main objective of this study involves the enlisting of therapeutically significant plant species, their medicinal properties, and methods of preparation of the herbal formulations used by the inhabitants of Bajwat to treat ailments. A bibliographical comparison of the floral diversity of the studied area with the previously published scientific literature from the district Sialkot and with the Herbal Pharmacopeia of Pakistan was performed to determine if the species were reported earlier with similar ethnomedicinal preparations at a regional or national level or if it is reported for the first time in this context.

Study Area and Climate.
Bajwat is a small village that is located in the district Sialkot, province of Punjab, Pakistan. Sialkot highlights a humid subtropical atmosphere according to the Köppen-Geiger atmosphere classification, with four seasons [18]. e postmonsoon (rainfall season) season from mid-September to mid-November stays hot during the daytime, yet the evenings are cooler with low humidity. In the winter from mid-November to March, days are moderate to cold, with once-in-a-while substantial rainfall. e temperature in winter may drop to 0°C, yet maximum is rarely under 15°C [11]. e precipitation in the region is very high, which sometimes results in flooding and washing of economically important crops. e normal precipitation is 965 mm [12]. e main source of income for the general population is the cultivation of crop plants such as wheat and rice. ey also raise livestock for dairy and profit. e people of the Bajwat have their ethnic linkage with the Kashmir province, and the majority of inhabitants are Muslims by religion. Bajwat Wildlife Area was declared as a sanctuary in 1964 by the Government of Pakistan to protect deer, wild pig, and nilgai from hunting and to prevent other serious threats to the flora. e Bajwat Wildlife Sanctuary is enlisted by the IUCN as a classification IV area, which incorporates a complex of common riverine living spaces, close to the Sialkot along the Chenab River and two of its tributaries, reaching out up to the Indian outskirts ( Figure 2). e area is usually inaccessible for a large population and is abandoned due to escalated tensions in the adjacent countries as the east side of the area moves along the Indian border [10]. e main realized danger is the unlawful chasing or hunting of animals, for which the zone has been announced as a Game Reserve and is protected by the Wildlife Department of the Government of Punjab, Pakistan. Below Bajwat is a place where the Chenab (river), Jammu (Occupied Kashmir, India), Tavvi (river), and Manavar Tavvi (river) meet at one spot. e Marala Headworks is situated in Bajwat's southwest corner, is a reservoir of the Chenab river water, and contributes to divert water in different territories of adjacent areas to irrigate field crops. e territory between these two channels is being created as the Marala Barrage Park [11]. ese special ecogeographic attributes along with our interest led us to choose this region for our study.

Selection of Informants.
e informants who had ethnopharmacological knowledge of medicinal plants and used crude formulations in the past or using in routine to treat ailments were selected [19].

Ethnopharmacological Survey on Medicinal Plants and Data Collection.
Open meetings were arranged, in which the individuals were met through casual discussion. Every member was interviewed separately, and the data were recorded with great care. e point of open meetings was to evoke people to talk openly, without any stress or anxiety and to collect information about the medicinal attributes of plants that they utilize or have utilized previously. e second step was semiorganized meetings to acquire detailed quantitative and qualitative data and to obtain a wide range of knowledge on explicit issues. e routine activities of the people of Bajwat Wildlife Sanctuary were observed during the study. A composed questionnaire was designed to collect data containing the name, sex, age, and address. In addition, the level of education of the interviewees and the spot and date of the meeting was recorded. e conversation with the interviewees was in Urdu and Punjabi languages. e questionnaires were filled by the researchers. e ethnopharmacological information consisting of the ethnomedicinal properties of the plant, the vernacular name, the plant parts utilized to prepare medicines, plant occurrence, credited therapeutic properties, and the techniques for preparing ethnopharmacological formulations was recorded [20,21].

Collection of Plant Samples and
eir Identification. e plant samples were collected from the Bajwat Wildlife Sanctuary with the help of local healers, herbalists, sellers, and informants. e study area was surveyed from January to the end of November 2019. e plants were identified by the author Waris Hussain with the help of the Flora of Pakistan. e herbarium sheets containing plant specimens were deposited in the herbarium of the Botany Department of PMAS-Arid Agriculture University, and accession numbers were allotted for future reference [22]. e correct and approved botanical names were enlisted throughout this study after verification from the Pakistan Plant Database (e-Flora of Pakistan) (https://www.tropicos.org/Project/ Pakistan) and Plant List (http://www.theplantlist.org/).

Quantitative Data Analysis.
e data collected from Bajwat was loaded on the Microsoft Office® program MS Excel® spreadsheet. e graphs were plotted, and the data were evaluated in the form of percentages and proportions. e species use reports (URs) per herbal formulation were also recorded, and the greater number of informants per herbal formulation was considered as an indicator of excessive use of that plant in crude drug preparations. e use reports are defined as the number of informants per herbal India Bajwat Wildlife Sanctuary 32°41′04.0″N 74°32′43.1″E

Iran
Sialkot China A f g h a n i s t a n

Results and Discussion
Bajwat Wildlife Sanctuary is a protected area that allows a great number of plant and animal species to proliferate freely without any human intervention. e studied area is a complex of different riverine and terrestrial territories that provide a great diversity of ecosystems and environmental conditions for the growth and reproduction of various varieties of plant species. To achieve the study goals and to test the hypothesis, 130 individuals, including 80 male and 50 female participants were interviewed. e local respondents belonged to different age groups ranging from 20-71/above. ey had different levels of education from primary to university, while 15% of the informants were enlisted as illiterate (Table 1). e seventh author was originally from one of the villages of Bajwat (Pul-Bajwan). He and his family helped greatly to meet local inhabitants and some known healers of the area. e plant vernacular names are also enlisted in Table 2. e vernacular names were similar in both Urdu and Punjabi languages, and it was not possible to differentiate them based on the language. Ten male herbalists (registered with the National Council of Tibb, Pakistan) were also reported from the studied area. It was observed that the male informants were having more detailed knowledge about the plants' local names, their identification, and collection, while the female participants were having significant information regarding the method of preparation of herbal formulations, and their mode of administration.

Medicinal Plants Diversity in Bajwat Wildlife Sanctuary.
e ethnopharmacological survey on medicinal plants of the Bajwat Wildlife Sanctuary led to the collection of 47 plant families and 114 medicinal plant species disseminated among 97 genera (Table 2). e distribution of the plant families to be used in ethnopharmacological medicines among the inhabitants of Bajwat Wildlife Sanctuary can be consulted in Figure 3. 10% of the total reported medicinal plant species belong to the family Leguminosae/Fabaceae. Poaceae and Solanaceae occupied the 2 nd rank (7%) in the context of the widely exploited medicinal plant species in the Bajwat Wildlife Sanctuary followed by Moraceae (6%), Asteraceae (5%), Apiaceae, Brassicaceae, Cucurbitaceae, Lamiaceae, and Rosaceae (4% each). Caesalpiniaceae was found to be 3% among the reported species. Fabaceae, Poaceae, Solanaceae, and Moraceae account for 30% of the total reported medicinal plant species of the area. e rest of the families were counted as 2%/<2% separately.  medicinal plant family countrywide (https://www.tropicos. org/Project/Pakistan). e lifeforms provide great diversity and physiognomic contrast to the ecological habitat. e most common life forms ( Figure 4) are reported in the ascending order for the preparation of herbal medicines such as herbs, shrubs, and trees. Some previous ethnobotanical studies on medicinal plants of the district Sialkot also reported herbs as the extensively utilized plant species to treat ailments of the local inhabitants [13,14,16,17].

e Traditional Source of Knowledge and Bibliographic
Comparison.

Plant Parts Used in Herbal erapy.
e different plant parts have different properties which help them to treat therapeutic ailments and likewise have significance in crude herbal formulations. 59% of the plants reported from the studied area were edible medicinal plants which show that these plants can be consumed in different forms to treat ailments, e.g., oral intake of drugs and food supplements to treat malnutrition, etc. while 41% of the medicinal plant species were not edible ( Figure 5(a)). It shows that most of the plants of the studied area have their utility as food along with their medicinal applications. Figure 5(b) shows that 51% of reported plant species have their only use in herbal medications while 21% of plants were used as a source of timber followed by 14% of ornamentals in addition to their other medicinal properties. 8% of the plants were utilized as animals' medicine. Most of the animal medicines were given as fodder to treat animals' digestive issues. Some plants were given to animals as a food supplement while some plants were reported effective to eradicate malnutrition. A herbal formulation was prepared from Argemone mexicana L. root extract and applied topically to treat animals' skin issues. 6% of the plants of the studied area were exploited as a source of dietary fiber to treat digestive ailments. e leaves, roots, wood or bark, flowers, herbaceous stem, fruit, and latex were found useful in a multitude of ethnopharmacological drugs, while some herbal formulations involved the use of whole plant material. Figure 5(c) shows that leaves and fruits play a significant role to make herbal remedies and stand out 19% separately from both   (7) and the constant rattling of mucus in bronchi (11). Extract and maceration are used on skin infections and bites (12). Chewing cloves helps to manage hypertension (15). e juice is used for kidney stones (12).  ) Seeds Stomach trouble, fever, malaria, and flu Oil is extracted, used for flu (7).

Evidence-Based Complementary and Alternative Medicine
Decoctions and the powder are used for stomach treatments (6). Seeds are used as masticatory (7). Leaves and seeds are used for malaria and fever (5).
Oral, ointment GH Common * Roots, seeds Improve vision, blood purification, anemia treatment Extracts are prepared and used for the treatment of anemia (20) and the purification of blood (10). Juice and extract are used to improve vision (30).
Oral HB Common *

Roots, leaves
Promotes abortion e root powder is used for the preparation of drugs to promote abortion (5) Oral, ornamental SH Rare * Catharanthus roseus

Leaves
Digestive disorders Leaves extracts and decoctions are prepared to relieve constipation (4) and digestive issues (4) Oral, ornamental HB Common
Ashes of dried fruit are used for the treatment of whooping cough (20).  (12), skin diseases (10) and poisonous infections (10). e root is used for ringworm treatment (10). Leaves boiled in oil are used as a balm which is used for anointing the head in eye complaints and to improve vision (12).   Leaves, fruit, seeds Digestive disorders, skin disorders, animals' treatment Leaves of this plant kill worms (10).
Extract of leaves is used to kill worms found in wounds of cattle (11). e ripened fruit is good for the proper functioning of the digestive system (11).
Oral HB Rare Evidence-Based Complementary and Alternative Medicine 18 Evidence-Based Complementary and Alternative Medicine categories which make 38% in total. e great proportion of leaves and fruits in herbal formulations show their significance because of the presence of various phytochemicals that are present in these plant parts, while another reason is the ease of availability of these parts. Wood or bark (12%), latex (10%), and flowers (10%) were reported likewise followed by roots (7%) and herbaceous stems (7%). 11% of the formulations reported the use of the whole plant body, while 5% reported the use of plants as a source of dietary fibers. Some formulations also reported the use of rhizomes or bulbs for the preparation of medicines. Some studies on the ethnomedicinal flora of Sialkot also reported the use of different plant parts in various herbal medicines dominated using leaves, fruits, and herbaceous stems, which is in favor of our study [13,14,16,17].

Preparation of Ethnopharmacological Formulations.
Preparation of crude plant formulations involves the use of potential phytometabolites to treat ailments. e potency of a herbal drug to cure disease depends greatly on the method of preparation that in actual is the manipulation of the  Evidence-Based Complementary and Alternative Medicine phyto-secondary metabolites to transfer their full potential from the plant part to the perilous part of the body. Table 2 reports the use of medicinal plants in crude herbal formulations against various pathophysiological conditions. is ethnopharmacological survey reported ( Figure 6) the use of plant extracts (32%) as a major method for the preparation of herbal drugs, while 11% of the herbal formulations were prepared in the form of decoctions. Some of the herbal remedies were used in the form of food (10%), oil (10%), and fresh plant materials (6%), while other herbal formulations involved cooking (5%) in the the form of food. e preparation of the ethnopharmacological formulations by the inhabitants of the studied area shows that the food medicinal portion is essential to treat the ailments. e people of Bajwat reported that the consumption of crude herbal drugs as food plays a significant role to treat ailments. e inhabitants of Bajwat were adding crude herbal formulations in their routine food and sometimes were preparing special formulations separately to treat ailments. Some of the inhabitants reported the inhalation of smoke (2%) of wood or other plant material to treat respiratory disorders such as bronchitis, cough, and asthma. A similar number (2%) of plant materials were used for the preparation of tincture. 8% of the total reported plant species were used in animal medicine because of their medicinal importance. Some of the plant species were given to animals as fodder to treat digestive disorders of livestock, to eradicate malnutrition, and used as a food supplement. Few herbal medicines were prepared and applied to animals' skin to cure dermal issues.
ere is no study reported previously from district Sialkot to determine the mode of preparation of herbal formulations by local inhabitants. Our results are contrary to some previously performed work from different territories of Pakistan that reported powder [25] as the best method for the preparation of herbal formulations because of fewer contamination chances of the powder and easy to preserve and reuse. e people of the Bajwat Wildlife Sanctuary claimed that the preparation of the extract is the most feasible and quick strategy that involves the simple method to prepare formulations and its consumption as fresh can increase the efficacy of a drug in case of any severe ailment.

Administration of the Ethnopharmacological Drugs.
Some of the inhabitants of Bajwat were well aware of the mode of administration and its significance. According to inhabitants, the mode of administration helps to deliver the drug efficiently and timely to the suffering part of the body. Figure 7 encodes various modes of administration of herbal formulations reported by the local inhabitants and healers. e oral route stands up with 69% of the total formulations reported. e oral route reported the ingestion of the fresh plant material powder, using teas, tinctures, or decoctions prepared from different plant parts. People of Bajwat also used oils as a laxative to cure digestive disorders, or sometimes different oils were taken orally to enhance vitality and to reduce feebleness and promote vigor. e oral intake of oil from some plant species was also reported as an aphrodisiac (e.g., Phoenix dactylifera L.). e second most common mode of administration was reported as the topical application (15%) of the plant crude drug. e topical administration of the plant material in the form of paste prepared from different plant parts was most common among the inhabitants of Bajwat to treat skin disorders such as acne, skin microbial infections, ringworms, and eczema and to treat burn marks. e topical administration also involved the cosmetic enhancement of the skin glow that includes the use of the paste of Aloe vera, Mentha arvensis aqueous extract spray, and Azadirachta indica paste to cure acne and other skin ailments. e cataplasm makes 7% of the total reported plant species from the study area that involves the maceration of different plant parts such as root, stem, and leaves and applied on the skin to treat different types of skin wounds and different degrees of burns and disorders. e cataplasm was also reported to play a futile role to act as an antidote against the stings of snakes, insects, and other organisms. e ointments also contribute to 7% of the reported mode of administration. Most of the ointments were extracted in the form of oils and massaged on the skin surface to cure joint pain and to promote strength among bones and muscles. Very few of the plant materials were smoked (2%) to cure various respiratory disorders such as asthma, cough, bronchitis, and emphysema. Some of the plant parts such as leaves and flowers were smoked as a narcotic and for recreation such as Nicotiana tabacum, while some were used as tranquilizers  and sedatives. e oral administration was reported extensively in Pakistan Herbal Pharmacopeia as the major mode of administration of herbal formulations [8]. Some studies performed in the nearby territories of Pakistan [26,27] also reported oral administration as the most common method of administration of herbal drugs.

Diseases and Pathological Conditions.
e digestive disorders were reported most common ailments of the inhabitants of Bajwat which account for 21% of the total disorders cured (Figure 8). Commonly reported digestive disorders include diarrhea, stomach pain, heart-burning issues, nausea, constipation, abdominal bloating, dyspepsia, and vomiting. Different ailments were categorized according to the WHO International Classification of Primary Care, 2 nd Edition, which is used worldwide. Skin disorders were found common among the inhabitants to be treated by crude formulations, and 16% of the total ailments cured marked to this category. e most common skin ailments among the inhabitants of Bajwat were reported to be eczema, skin boils, acne, skin burns, skin infections, ringworm, and warts. 14% of respiratory disorders were cured such as cough, asthma, bronchitis, emphysema, and shortness of breath. e rest of the different ailments including fever and malaria (12%), urinary disorders (10%), hepatic ailments (9%), reproductive disorders (7%), cardiac disorders (6%), and neurological disorders (5%) were cured by using different herbal formulations.    oral and topical administrations to treat skin disorders. ere was no study performed previously to report the health disorders of the people of the district Sialkot or inhabitants of the Bajwat Wildlife Sanctuary.

Side Effects of Poisonous Medicinal Plants.
e inhabitants of Bajwat reported that most of the plants of the sanctuary do not have any harmful effects. A few species anyway were having dangerous impacts and the nearby individuals keep up broad information about the distinctive poisonous and nonharmful medicinal plants. For instance, the juice of the seeds of Datura innoxia Mill. [28], Datura metel L. [29], and Ricinus communis L. [30] is known to be profoundly harmful. A few plants were reported to have poisonous impacts on both humans and livestock, for example, Calotropis procera (Ait.) and Euphorbia helioscopia L. e latex of the two plants causes harmful impacts through eye and skin contact. Some studies reported that the use of the limited dose of the latex of Euphorbia is beneficial to treat warts and wounds, but excessive and unlimited use of latex can be carcinogenic [21]. Parthenium hysterophorus L. was also reported to be a poisonous plant by local healers and some inhabitants and can cause severe respiratory ailments and was found growing excessively along the roadsides. Although some people in the local market were found using Parthenium hysterophorus L. to decorate flower bouquets, Maleki and Akhani [21] also reported some of the plants mentioned above as poisonous plants from Mountain Taftan, Iran, which is located at the border of Pakistan's southwestern province, Balochistan.
e Royal Botanical Garden's annual report [31], stated 8% of poisonous plants out of the total documented plant uses. However, in the Bajwat Wildlife Sanctuary only 6 plants were reported poisonous which makes 5.26% of the total reported plant species of the study area.

Plants Enlisted in the IUCN Red List of reatened Plant
Species.
e IUCN Red List of reatened Species provides significant information about the evidence-based extinction and risk assessments of individual plant species. It also contributes to understanding the forces that are responsible for the extinction of species and also helps in management interventions to overcome or delay the extinction. Despite critical evaluation of species at risk of extinction, it is believed that many plant species will be extinct before they have been recognized as being at risk of extinction [32]. Determination of plant species' current status in the IUCN Red List of reatened Species can greatly help to protect the threatened ethnomedicinal flora of a region. According to the IUCN Red List of reatened Plant Species, more than 3200 species are threatened with extinction, which makes about 27% of the total assessed species. Eight out of the total 114 plant species were listed in the IUCN Red List as least concern (LC), including Bauhinia variegate * , Cyperus rotundus L., Cassia fistula L. * , Centella asiatica (L.) Urb. * , Echinochloa crus-galli (L.) P. Beauv, Eclipta prostrata L., Vitis vinifera L. * , and Vitex negundo L. while Eucalyptus camaldulensis Dehnh. * was enlisted as near-threatened (NT). e occurrence of plant species in Bajwat Wildlife Sanctuary was also observed, and 19 species were found rarely distributed, which makes 17% of the total reported plant species, while 5 species ( * placed at the end of their name) out of 19 rarely distributed species were enlisted in the IUCN Red List of reatened Species (Table 2).

Conclusion
is study involves the first-ever extensive ethnopharmacological investigation to report the medicinal plants of the Bajwat Wildlife Sanctuary, which is a complex riverine biological ecosystem. We have reported 114 medicinal plant species. e extract preparation (32%) was reported as the most basic strategy for making herbal formulations, while oral intake (69%) was observed most common for the administration of drugs among the local community. e highest number of URs against digestive disorders (290∼15%) and skin disorders (279∼14%) show that these are the most common problems among the local inhabitants. e stomach-related issues were seen generally regular among local people and were cured by crude ethnopharmacological formulations.
is study embraces the ethnopharmacological knowledge of local healers and inhabitants and reports the exchange of data from generation to generation because of strong communication in families and neighboring communities.
e investigation of the ethnomedicinal information and their comparison with past reports from the district Sialkot, and Pakistan Herbal Pharmacopeia endorses the enlisted knowledge of local healers and inhabitants to prepare formulations against multiple disease categories. About 75% of the enlisted medicinal plant species were not reported previously in

Data Availability
e data used to support the findings of this study are included within the article.

Ethical Approval
is study does not involve any human or animal experiments. e study was approved by the University Bioethical Committee, and the data were recorded from local inhabitants/participants after oral consent.

Conflicts of Interest
e authors declare no conflicts of interest.

Authors' Contributions
BJ, WI, MS, HABA, and WH devised the study, collected and identified plant specimens, and interviewed inhabitants. BJ wrote and revised the manuscript and analyzed data. BJ, WI, MS, HABA, AS, and ZRM were added to the subsequent drafts. e authors read and finalized the manuscript for submission. Sidra Ahsan Shah, Wajeeha Iqbal, Muneeba Sheraz, and Bilal Javed are equally contributed first authors.