PAMK Relieves LPS-Induced Enteritis and Improves Intestinal Flora Disorder in Goslings

Polysaccharide of Atractylodes macrocephala Koidz (PAMK) is a biologically active component of Atractylodes macrocephala, which has the effect of maintaining the immune homeostasis of the body. Therefore, this study constructed a model of PAMK to relieve LPS-induced gosling enteritis and observed the morphological changes of the small intestine after HE staining. ELISA was used to detect serum CRP, IL-1β, IL-6, and TNF-α levels; immunohistochemistry was used to detect the positive rate of IgA in the small intestine; TLR4, occludin, ZO-1, cytokines, and immunoglobulin mRNA expression in the small intestine were detected by qPCR; and intestinal flora of gosling excrement was analyzed by 16S rDNA sequencing to analyze the protective effect of PAMK on goslings enteritis and the impact on intestinal flora. The results showed that PAMK relieves LPS-induced gosling enteritis by maintaining the small intestine morphology, cytokine, tight junctions, and immunoglobulin relatively stable and improving the disorder of intestinal flora.


Introduction
In the brooding stage of geese, due to the imperfection of immune function, goslings are vulnerable to various pathogenic microorganisms, leading to the occurrence of various intestinal diseases and seriously affecting the healthy development of the goslings. However, antibiotics, hormones, and other chemical drugs very easily cause drug residue in poultry products. erefore, the development of antibiotic replacement products, which can effectively improve the immunity of goslings without residues and toxic and side effects, has become one of the hot issues of concern. Polysaccharide of Atractylodes macrocephala Koidz (PAMK) is the main biological activity component of Atractylodes macrocephala. Studies have confirmed that it has anti-inflammatory, antioxidant, antitumor, antistress, and immune-enhancing effects [1][2][3]. PAMK regulates immune function by promoting the development of immune organs, the proliferation and activation of immune cells, the secretion of cytokines, and the maintenance of the steady state of the immune system [4][5][6]. erefore, the regulation mechanism of PAMK on gosling enteritis is worthy of further study. e intestinal mucosal immune system is mainly composed of mucosa-associated lymphoid tissue (MALT), including mesenteric lymph nodes, aggregate lymph nodules, and lymphocytes dispersed in the intestinal lamina propria and intestinal epithelium. It plays an important role in maintaining intestinal flora balance and intestinal barrier function and repairing intestinal mucosal epithelium [7]. e strength of intestinal mucosal immune function is directly related to the structural integrity of MALT and the secretion of sIgA. SIgA mainly exists in the relative external environment of the body's digestive tract, lymphatic vessels, etc. It can prevent bacteria from attaching to the surface of the intestinal mucosa, effectively prevent bacteria from passing through the intestinal mucosa, and play an important role in strengthening the intestinal immune barrier [8][9][10]. e epithelial cells of the intestinal mucosa also have a barrier function, and the tight junctions of epithelial cells, such as occludin and zonula occludens-1 (ZO-1), constitute a tight junction structure. When the tight junction is closed, it can prevent the passage of bacteria or toxic macromolecules. Studies have shown that polysaccharides of traditional Chinese medicine have an important role in enhancing intestinal immunity, such as Ganoderma atrum polysaccharide, Codonopsis pilosula polysaccharide, and Dendrobium officinale polysaccharides have significant effects on repairing intestinal mucosal structure and enhancing intestinal mucosal immunity [10][11][12]. Atractylenolide I and PAMK have been shown to stimulate the migration and proliferation of intestinal epithelial cells and have a significant effect on repairing intestinal mucosal epithelium [13][14][15]. Studies have also reported that PAMK can enhance intestinal mucosal immune function by increasing the mRNA expression of duodenal cytokines and IgA in mice [16]. erefore, PAMK has a significant effect on enhancing the intestinal mucosal immune function.
e intestinal microbiota is a biological barrier, forming an interdependent and interacting microecosystem with the host. e composition and ratio of various microbial species in the intestinal tract are generally relatively stable, and the bacterial species have a restrictive and interdependent relationship. e influence of intestinal flora on the immune function of intestinal mucosa is double-sided [17,18]. When the flora is in a relatively stable state, it can form a biological barrier to prevent the displacement of bacteria and endotoxins and the colonization of exogenous pathogens. When the symbiosis of bacteria and pathogenic bacteria is imbalanced, the intestinal biological barrier function is lost, which is an important cause of intestinal diseases. erefore, changes in intestinal flora play an important role in mucosal immune function.
Traditional Chinese medicine polysaccharides are foodborne substances that can directly enter the body's digestive system, and some of them can be directly decomposed and used as nutrients by gastrointestinal microorganisms. erefore, traditional Chinese medicine polysaccharides must be closely related to intestinal flora. Polysaccharide of Atractylodes macrocephala Koidz (PAMK) is a biologically active component of Atractylodes macrocephala. Atractylodes macrocephala, belonging to Asteraceae and Atractylodes perennial herb, has diuretic, hypoglycemic, antitumor, antibacterial, and vasodilator effects. Atractylodes macrocephala is commonly used for diarrhea and other diseases and has certain effects on the treatment of gastrointestinal dysfunction [19]. Further research on the effects of Atractylodes macrocephala on intestinal flora found that PAMK can improve intestinal flora imbalance and have a significant effect on the treatment of colitis in mice [20]. However, the current research has not yet fully explained the effect of PAMK on the intestinal flora homeostasis. erefore, this study built a model of PAMK to relieve LPS-induced gosling enteritis and detected immune indicators in serum and intestinal flora. Besides, 16S rDNA sequencing was performed on excrement to study the alleviation effect of PAMK on gosling enteritis and its relationship with intestinal flora.

Experimental Animals.
One-day-old goslings (Anser cygnoides) were purchased from Guangdong Qingyuan Jinyufeng Goose Co., Ltd, which is a professional goose hatching company, and housed in a specific pathogen-free environment. Goslings had free access to food (including the same amount of vegetables) and water and were treated humanely, and the experiments received prior ethical approval in accordance with Zhongkai University of Agriculture and Engineering and under the approved protocol number SRM-11.
PAMK was diluted with ddH 2 O and sprayed on the feed according to the concentration of 400 mg·kg −1 . LPS (Escherichia coli 055: B5, Lot: L2880) was purchased from Sigma and fully dissolved in sterile physiological saline for use. Endotoxin detection kit (chromogenic end point assay) was purchased from Fuzhou Xinbei Biochemical Industrial Co., Ltd. Enzyme-Linked Immunosorbent Assay (ELISA) kit was purchased from Nanjing Jiancheng Bioengineering Institute. TRIzol reagent (Invitrogen, China, Lot: 15596026), first-strand complementary (cDNA) kit (Invitrogen, China, Lot: K1622), and PowerUp SYBR Green Master Mix (Invitrogen, China, Lot: A25918) were used for quantitative real-time PCR analysis.

Treatment of Animals.
Two hundred goslings (1-dayold, with half males and half females) were randomly divided into four groups (n � 50 per group) (see Table 1). e FBC1 and FBL groups were fed with normal diets, whereas the FBP and FBPL group were fed with 400 mg·kg −1 PAMK. In addition, the FBC1 and FBP groups were injected with the same amount of saline, while the FBL and FBPL groups were injected with 2 mg·kg −1 ·BW LPS per day at the 24th, 26th, and 28th day of age to construct inflammation models. Blood, excrement, duodenum, jejunum, and ileum were collected after 1 hour of injection on the 28th day. All organs 2 Evidence-Based Complementary and Alternative Medicine were placed in liquid nitrogen immediately and stored at −80°C until used.

HE Staining.
e duodenum, jejunum, and ileum were fixed in paraffin. e paraffin-fixed blocks were serially sectioned into 5 to 6 μm-thick coronal slices. For routine histological examination, the paraffin sections were stained with HE. HE-stained slices were analyzed under a Nikon fluorescence microscope (Nikon, Tokyo, Japan). e intestinal villus length and crypt depth of each group of intestines were measured using MShot Image Analysis System v1.0, and the V/C value (villus length/crypt depth) was calculated.

Serum Endotoxin, CRP, and Proinflammatory Factors
Assay. Venous blood was collected to separate the serum. e levels of endotoxin in serum were measured using endotoxin detection kit according to the manufacturer's instruction. e levels of CRP, IL-1β, IL-6, and TNF-α in serum were measured using ELISA kit according to the manufacturer's instruction.

IgA Expression in the Duodenum, Jejunum, and Ileum
Was Detected by Immunohistochemistry. Paraffin sections were incubated with IgA primary antibody (Bethyl, Lot: A30-103P), operated according to the immunohistochemistry kit (purchased from DAKO ChemMate EnVision, Lot: K5007), and observed using a microscope (Nikon, ECLIPSE E100). Five 400-fold magnification fields were selected for each section to calculate the number of positive IgA-secreting cells.

Quantitative Real-Time PCR Analysis.
Specific primers matching reverse-transcribed mRNA were designed ( Table 2). e procedure for quantitative PCR was similar to the one published by Yao et al. [21]. Total RNA was isolated from individual thymuses using the TRIzol reagent according to the manufacturer's instructions. First-strand complementary (cDNA) was synthesized using oligo dT primers and SuperScript II reverse transcriptase, according to the manufacturer's instructions. QPCR was performed on an ABI PRISM 7500 detection system (Applied Biosystems, USA). e PCR procedure steps included 95°C for 30 s, followed by 40 cycles of 95°C for 15 s, 60°C for 30 s, and 60°C for 30 s.

16S rDNA Sequencing.
e gosling excrement was collected and sent to Shenzhen Hengchuang Gene Technology Co., Ltd. for total DNA extraction, PCR amplification, and 16S rDNA sequencing. Eight replicates were set for each group. After the data analysis was performed, three samples with poor reproducibility were removed. e target DNA obtained by PCR amplification was amplified by the 16S rDNA V4 region, and the raw data was obtained by Illumina HiSeq 2500 PE250 sequencing platform. After the data was split, PE reads were spliced, tags went to the chimera sequence, etc., some low-quality data was removed and the final valid data was obtained. OTU clustering and species annotation, alpha diversity, beta diversity, and statistical analysis of differences between groups were performed on the data.

Statistics.
e results were expressed as means ± SD. All the qPCR reactions were performed in triplicate, and the relative levels were measured in terms of threshold cycle value (Ct) and were normalized using equation 2 −∆∆Ct . Statistical analysis of all data was performed using SPSS (version 16, SPSS Inc.). e statistical significance (P < 0.05) was evaluated by one-way ANOVA with simultaneous multiple comparisons among different groups. Figure 1(a) shows that there are a strong and wide absorption peak near 3600 cm−1-3200 cm−1, which is O-H bond; a strong absorption peak near 2910 cm−1, which is O-H bond; and a peak around 1650 cm−1.

Infrared Spectrum Results of PAMK.
ere are a stretching vibration absorption peak, which is C�O bond; deformation vibration absorption peaks near 1450 cm−1 and 1375 cm−1, which is C-H bond; and a relatively wide absorption peak near 1100 cm−1, which is C-O bond for pyran ring structure.

PAMK Protects Intestinal Morphology from LPS in Goslings.
e results in Figure 1(b) show that the villi of the FBC1 and FBP are compact, the crypts are deep, the tissues are dense, and the intestinal structure is intact. In the FBL, the villi were severely broken and the defect was severe. e number of cells in the villus epithelial layer was reduced, the cells were vacuolated, and the connective tissue in the submucosa was loose. Compared with the FBL, the FBPL group's tissue structure was significantly reduced. e number of villous epithelial cells was similar to that of the FBC1 group. e width and number of villi were significantly increased. e overall morphology was close to the FBC1 group, but the lymphocyte infiltration was indistinguishable from the FBL group. erefore, after feeding with PAMK, it has obvious repairing effect on LPS-induced injury, which can make the morphology and quantity of villi close to FBC1 and reduce the number of goblet cells in intestinal crypt. Figure 1(c) shows that the villi of the FBC1 and FBP groups are neatly arranged, and the number of goblet cells in the villi and crypt is significantly larger than that in the duodenum. In the FBL group, the epithelial detachment of jejunum villi was severe, the number of intestinal glands Evidence-Based Complementary and Alternative Medicine increased, and the number of lymphocytes was more. e number of goblet cells in the villus epithelium was significantly higher than that in the FBC1 group. e jejunum morphology of the FBPL group was close to that of the FBC1 group. e villi were neatly arranged. e goblet cells in the villus epithelium were significantly higher than those in the FBC1 group. e tissue compaction recovery was better, the gut gland structure was clear, the thickness was normal, the congestion was reduced, and the lymphatic infiltration phenomenon and FBL were observed. e groups are similar. In summary, PAMK has no obvious effect on the structure of jejunum, but it also has a good ability to repair the jejunum under LPS injury, which can maintain the integrity of intestinal villi and increase the length of villi.
As shown in Figure 1(d), compared with the FBC1 group, the ileal villi of the FBP group were broadened, and the number of goblet cells in the villus epithelial area increased, evenly distributed between the aligned columnar cells. In the FBL group, the villi became thicker and shorter, the gap increased, the epithelial detachment of the villus was severe, the vacuolar cells increased, the number of the goblet cells increased, the cells in the lamina propria was increased, and the number of lymphocytes increased. Lymphocyte infiltration and hyperemia are evident, and the toroidal muscle layer becomes thinner. In the FBPL, the length and width of the ileum were close to those of the FBC1 group, but there was still epithelial shedding at the top of the villus. In addition, the number of goblet cells in the villi was close to  that of the FBC1 group, and the lamina propria structure returned to normal. e thickness of the gut was also close to that of the FBC1 group. In summary, PAMK has a significant effect on the development of ileal villi in normal goslings, and this effect only occurs in the ileum. In addition, PAMK has a significant repair effect on the damage caused by LPS. Except for the epithelial detachment at the top of the villus, the other lesions can be relieved obviously, and the villus morphology returns to normal. By calculating the villus length/crypt depth (V/C) values of the duodenum, jejunum, and ileum ( Figure 1(e)), it was found that the V/C values of the FBL group in the jejunum and ileum were significantly lower than those in the FBC1 group (P < 0.05), indicating that LPS can cause a decrease in the V/C value. In addition, the V/C values of the FBP in the duodenum, jejunum, and ileum were significantly higher than those in the FBC1 group (P < 0.05), indicating that PAMK can increase the V/C value of the small intestine and promote the development of small intestine villi. At the same time, the V/C values of the FBPL group in the duodenum and ileum were significantly higher than those in the FBL (P < 0.05), indicating that PAMK has protective and repairing effects on the duodenal and ileal mucosa of LPS injury.

PAMK Alleviates Intestinal Inflammation Induced by LPS in Goslings.
e results showed that ( Figure 2) the levels of endotoxin, CRP, and proinflammatory factors (IL-1β, IL-6, TNF-α) in the serum of FBL group were significantly increased compared with FBC1 group (P < 0.05). It means that LPS significantly enhanced the inflammation of goslings and successfully constructed an LPS gosling inflammation model. Compared with the FBC1, the serum levels of endotoxin in the goslings of the FBP were significantly decreased, and IL-6 was significantly increased (P < 0.05), while CRP, IL-1β, and TNF-α were not significantly changed (P > 0.05). However, the levels of endotoxin, CRP, and proinflammatory factors (IL-1β, IL-6, TNF-α) in the FBPL were significantly lower than those in the FBL (P < 0.05). Among them, CRP and IL-1β could be restored to the level of FBC1, while the levels of endotoxin, IL-6, and TNF-α were still significantly higher than those of FBC1 group (P < 0.05).
e results showed that PAMK can not only reduce the endotoxin in healthy goslings, but also protect the goslings from LPS, reducing the inflammation in the gosling induced by LPS. e results of duodenum immunohistochemistry showed (Figures 3(a) and 3(b) (A)) that there were no obvious IgA-secreting cells in the intestinal epithelial cells and crypts of the FBC1 group, indicating that the sIgA secretion ability of the duodenum was weak. Compared with FBC1 group, IgA-secreting cells in FBP group increased significantly (P < 0.05), mainly distributed in intestinal villus epithelial cells, indicating that PAMK can promote the increase of IgA-secreting cells in the villus epithelium of duodenum, and the secretion of sIgA is enhanced. In addition, the number of IgA-secreting cells in the duodenal villi of the FBL group was significantly higher than that of the FBC1 group and the FBP group (P < 0.05), and the number of IgA-secreting cells was the highest among the four groups, indicating that IgA cells were significantly increased and LPS significantly promoted the secretion capacity of sIgA. However, the number of IgA-secreting cells in the FBPL group was significantly lower than that in the FBL group (P < 0.05), and the IgA-secreting cells in the crypt were less than those in the FBL group, indicating that PAMK can alleviate the IgA-secreting cells compensatory increase caused by LPS in the duodenum. e results of jejunal immunohistochemistry showed (Figures 3(a) and 3(b) (B)) that the jejunum of the four groups of goslings contained more IgA-secreting cells. Compared with FBC1, the number of IgA-secreting cells in the jejunum of FBP group and FBL group was significantly increased (P < 0.05), the staining was deeper, and the cell morphology and contour were more obvious. e number of IgA-secreting cells in the jejunum of the FBPL group was not significantly different from that of the FBC1 group (P > 0.05), and the morphology of IgA-secreting cells was more similar to that of the FBC1 group. Besides, both PAMK and LPS could significantly increase (P < 0.05) the number of jejunal IgA-secreting cells and promote the secretion of sIgA. After cotreatment with PAMK and LPS, the number of jejunal IgA-secreting cells was close to that of FBC1 group. e results of ileal immunohistochemical staining showed (Figures 3(a) and 3(b) (C)) that the IgA-secreting cells in FBC1 group were mainly distributed in intestinal villus epithelial cells, and the IgA-secreting cells in FBP group were significantly decreased compared with FBC1 group (P < 0.05). Compared with the FBC1 group, the IgAsecreting cells of the FBL group were significantly increased (P < 0.05) and mainly distributed in the intestinal mucosa epithelium and crypt. e IgA-secreting cells in the FBPL group were significantly lower than those in the FBL group (P < 0.05), and there was no significant difference between the FBP group and the FBC1 group (P > 0.05). e results showed that PAMK reduced the number of IgA-secreting cells in the ileum of healthy goslings and also decreased the increase of IgA-secreting cells in the ileum caused by LPS.
In summary, LPS caused an increase in IgA-secreting cells in the duodenum, jejunum, and ileum, promoted small intestine to secrete sIgA, and enhanced the immune barrier function of intestinal mucosal immunity. However, whether this is due to the compensatory increase of IgA secretory cells caused by the inflammation of LPS still needs further discussion. In addition, PAMK has the effect of increasing the number of IgA-secreting cells in the duodenum and jejunum and also inhibits the significant increase in the number of IgA-secreting cells in the small intestine caused by LPS. erefore, PAMK is a dynamic and stable regulation of intestinal mucosal immune function.
e relative mRNA expression of IgA in the gosling small intestine showed (Figure 4(a) (A)) that the IgA mRNA expression in the duodenum, jejunum, and ileum of FBL was significantly (P < 0.05) higher than that of FBC1, indicating that LPS would significantly increase the expression of IgA mRNA in the small intestine. In addition, the relative mRNA expression of IgA in duodenum and jejunum of FBP was Evidence-Based Complementary and Alternative Medicine  Evidence-Based Complementary and Alternative Medicine significantly (P < 0.05) higher than that of FBC1, but in duodenum and ileum it was significantly (P < 0.05) lower than that of FBL. At the same time, relative mRNA expression of IgA in duodenum, jejunum, and ileum of FBPL was significantly (P < 0.05) lower than that of FBL. is shows that PAMK can promote the expression of IgA mRNA in the small intestine of healthy goslings, but it can also alleviate the excessive increase of IgA mRNA in the small intestine of goslings treated by LPS and make the expression of IgA closer to that of healthy goslings. e relative mRNA expression of IgG in the goslings small intestine tissue showed (Figure 4(a) (B)) that the IgG mRNA expression in the jejunum of FBL was significantly (P < 0.05) higher than that of FBC1, but in duodenum and ileum it was significantly (P < 0.05) lower. is shows that LPS can significantly change the IgG mRNA expression in the small intestine. In addition, FBP duodenum, jejunum, and ileum IgG mRNA expression were significantly (P < 0.05) higher than that of FBC1, and the expression in duodenum and ileum of FBPL was significantly (P < 0.05) higher than that of FBL. is shows that PAMK can promote the expression of IgG mRNA in the small intestine of healthy goslings, but it can also alleviate the change of IgG mRNA expression in the small intestine of goslings treated by LPS, so that the expression of IgA is closer to that of healthy goslings. e relative mRNA expression of IgM in the small intestine tissue of goslings showed (Figure 4(a) (C)) that the mRNA expression of IgM in duodenum, jejunum, and ileum of FBL was significantly (P < 0.05) higher than that of FBC1, indicating that LPS would significantly (P < 0.05) increase the mRNA expression of IgM in the small intestine. In addition, the mRNA expression of IgM in the jejunum of FBP was significantly (P < 0.05) higher than that of FBC1, but in the ileum it was significantly (P < 0.05) lower than that of FBC1. At the same time, mRNA expression of IgM in duodenum, jejunum, and ileum of FBPL was significantly (P < 0.05) lower than that of FBL. is shows that PAMK can alleviate the excessive increase of IgM mRNA expression in the small intestine of goslings treated with LPS and make the expression of IgM closer to that of healthy goslings. e relative mRNA expression of CRP in the small intestine tissues of goslings showed (Figure 4(b) (A)) that the expression of CRP mRNA of FBL in duodenum, jejunum, and ileum was significantly (P < 0.05) higher than that of FBC1, indicating that LPS increased the expression of CRP mRNA in the small intestine and promoted inflammation happened. In addition, the CRP mRNA expression in duodenum and jejunum of FBP was significantly (P < 0.05) higher than that of FBC1, while it was significantly (P < 0.05) lower than that of FBC1. However, the expression of CRP mRNA in duodenum, jejunum, and ileum of FBPL was significantly (P < 0.05) lower than that of FBL. is shows that PAMK can alleviate the significant increase of CRP mRNA expression in the small intestine of goslings treated with LPS, so that the expression of CRP is closer to that of healthy goslings. e relative mRNA expression of IL-1β in the small intestine tissues of goslings showed (Figure 4(b) (B)) that the mRNA expression of IL-1β of FBL in duodenum, jejunum, and ileum was significantly (P < 0.05) higher than that of FBC1. However, the mRNA expression of FBPL in duodenum, jejunum, and ileum had no significant (P > 0.05) difference from that of FBC1. is shows that PAMK can alleviate the significant increase of IL-1β mRNA expression in the small intestine of goslings treated with LPS, so that the expression of IL-1β is closer to that of healthy goslings. e relative mRNA expression of IL-4 in the small intestine tissues of goslings showed (Figure 4(b) (C)) that the mRNA expression of IL-4 of FBL in duodenum and ileum was significantly (P < 0.05) lower than that of other groups. However, expression in FBPL was significantly (P < 0.05) higher than that in FBL and had no significant (P > 0.05) difference from that in FBC1. Besides, mRNA expression of IL-4 of FBP and FBPL in jejunum was significantly lower than that of FBC1. e relative mRNA expression of IL-6 in the small intestine tissues of goslings showed (Figure 4(b) (D)) that the mRNA expression of IL-6 of FBL in jejunum and ileum was significantly (P < 0.05) higher than that of FBC1. However, expression in FBPL was significantly (P < 0.05) lower than that in FBL. e mRNA expression of IL-6 of FBP in duodenum was significantly (P < 0.05) lower than that of FBC1 and had no significant (P > 0.05) difference from other groups. e relative mRNA expression of IL-10 in the small intestine tissues of goslings showed (Figure 4(b) (E)) that the mRNA expression of IL-10 of FBL in duodenum and ileum was significantly (P < 0.05) lower than that of FBC1. However, expression in FBPL was significantly (P < 0.05) higher than that in FBL. Besides, the mRNA expression of IL-10 of FBP and FBPL in jejunum was significantly (P < 0.05) higher than that of the other two groups. e relative mRNA expression of IFN-c in the small intestine tissues of goslings showed (Figure 4(b) (F)) that the mRNA expression of IFN-c of FBL in duodenum and ileum was significantly (P < 0.05) lower than that of FBC1. However, expression in FBPL was significantly (P < 0.05) higher than that in FBL. Besides, the mRNA expression of IFN-c of FBL and FBPL in jejunum was significantly (P < 0.05) higher than that of the other two groups. e results showed (Figure 4(c) (A)) that the mRNA expression levels of TLR4 in the duodenum, jejunum, and ileum of the FBL group were significantly higher than those in the FBC1 group (P < 0.05), indicating that LPS upregulated the expression of TLR4 in the small intestine, and the LPS model was built successfully. Compared with FBC1, the mRNA expression of TLR4 in the duodenum of FBP group was significantly decreased (P > 0.05), but there was no significant change in jejunum and ileum. However, the mRNA expression of TLR4 in the duodenum, jejunum, and ileum of the FBPL group was significantly decreased (P < 0.05) but could not be reduced to the level of FBC1. e results indicated that PAMK can inhibit the TLR4 inflammatory signaling pathway in LPS-treated goslings and has a certain anti-inflammatory effect. e results showed (Figures 4(c) (B) and 4(c) (C)) that the mRNA expression levels of occludin and ZO-1 in the duodenum, jejunum, and ileum of the FBL group were significantly lower than those in the FBC1 group (P < 0.05).
is indicated that LPS damaged the mucosal barrier function of the small intestine of goslings. Compared with 8 Evidence-Based Complementary and Alternative Medicine the FBC1 group, the mRNA expression levels of occludin and ZO-1 in the duodenum, jejunum, and ileum of the FBP group were significantly increased (P < 0.05). e mRNA expression levels of occludin and ZO-1 in goslings of FBPL group returned to normal levels, indicating that PAMK can alleviate the damage of LPS on the intestinal mucosal barrier of goslings. In conclusion, PAMK can promote the mRNA expression of tight junction occludin and ZO-1 in the small intestine of healthy and enteric inflammatory goslings, indicating that PAMK has a protective effect on the intestinal tight junction of the small intestine.

PAMK Improves LPS-Induced Gut Microbial Disorders in
Gosling. As shown in Figure 5(a), there is no significant difference in the sequence composition ratio of each sample at the boundaries, gates, classes, orders, and families. ere are differences between groups at the level of genus and species. e results showed that the sequence composition of the FBL decreased at the genus level, and the proportion at the species level increased. e sequence composition ratio of the other three groups was similar at the genus and species level, and there was no significant change.
Based on the species annotation and abundance information of all samples at the genus level, the abundance information of the genus level in each sample was selected to draw a histogram, and the difference in abundance levels between the groups was analyzed. e results ( Figure 5(b)) showed that the species abundance of the FBC1, FBP, and FBPL group was lower at the genus level. However, the bacterial classification of the FBL group was significantly different from that of the other three groups, and the species abundance was significantly higher than the other three groups.
In order to find the aggregation law of species or samples, the abundance information of the genus level in each sample was selected to draw a heatmap and clustered from the classification information and the difference between the samples. e results (Figure 5(c)) show that the samples in the FBL group are clustered together, indicating that the sample abundance of the group is very similar and the repeatability is good. In addition, samples from the FBL group had high Z values in many annotated species classifications, indicating a higher species abundance in the LPS group. However, the clustering results of the other three groups did not show differences, indicating that the abundances of the three groups were very similar and the abundance was low. From the above results, LPS treatment can significantly increase the species abundance of gosling gut microbes, while adding 400 mg/kg PAMK can maintain the relative stability of microbial species abundance in the gut of goslings, making it closer to healthy goslings.
Alpha diversity is an analysis of the richness and uniformity of species composition in a sample, usually assessed using indicators such as observed species, PD whole tree, Shannon, and Chao1. e more complex the diversity of a sample, the higher the index. e results showed (Figure 6(a)) that the Shannon, Chao1, observed species, and PD whole tree indices in the FBL group were the highest among the four groups and were significantly different from those in the FBC1 and FBPL groups (P < 0.05). is means that the diversity of microbial species in the gut of the FBL group is very complicated, and the addition of 400 mg/kg PAMK can maintain the stability of microbial species diversity in the intestines of the goslings.
Beta diversity was used to compare the microbial community composition between different samples. Bray-Curtis, weighted UniFrac, and unweighted UniFrac distance were used to estimate the microbial community structure differences between different samples based on the OTU abundance information of the samples. Beta diversity is mainly used to measure the coefficient of dissimilarity between two samples. e smaller the value, the smaller the difference in microbial community composition between the two samples. Figure 5(d) is a beta diversity index heatmap based on the weighted UniFrac distance, Bray-Curtis distance, and unweighted UniFrac distance for each sample. e results showed that the individual coefficients of each sample in the FBC1, FBP, and FBPL groups were relatively small, indicating that the species diversity between these samples was small. However, the differences in the FBL group and the other three groups were larger, indicating that the species diversity between the FBL group and the other three groups was large.
Principal Component Analysis (PCA) is a method of dimensionality reduction of multidimensional data to extract the most important elements and structures in the data. Principal Coordinate Analysis (PCoA) is a similar dimension reduction method to PCA, extracting the most important elements and structures from multidimensional data.
e results show ( Figure 6(b) (A)) that the first principal component of PCA accounts for 78.5% and the second principal component accounts for 10.5%, while the first principal component of PCoA results is 59% and the second principal component accounts for 18% (Figure 6(b)  (B)). In addition, both results showed that all samples in the FBC1 group, FBP group, and FBPL group were close to each other in the first principal component and farther away from each sample in the FBL group. is indicated that the species composition of FBC1 group, FBP group, and FBPL group was similar, while the species composition structure of FBL group was far from that of the other groups. e results showed that PAMK can maintain the stability of the gut microbial species composition and prevent the imbalance of intestinal microbial species composition caused by LPS.
e ANOSIM (analysis of similarities) is a nonparametric test used to test whether the difference between the groups is significantly greater than the difference within the group to determine whether the test is meaningful. e results of the ANOSIM mainly include the R value and the P value between the groups. e R value is within (−1, 1). e R value is greater than 0, indicating that the difference between the groups is significant. e R value is less than 0, indicating that the difference within the group is greater than the difference between the groups. e reliability of the statistical analysis was expressed by the P value, and P < 0.05 indicates that the statistics were significant. From the results in Table 3, there was a significant difference between the FBL group and the other three groups, but there was no  OTU_7  OTU_6  OTU_4  OTU_10  OTU_17  OTU_12  OTU_22  OTU_20  OTU_8  OTU_13  OTU_9  OTU_15  OTU_14  OTU_799  OTU_638  OTU_11  OTU_16  OTU_23  OTU_18  OTU_27  OTU_21  OTU_29  OTU_28  OTU_35  OTU_33  OTU_24  OTU_25  OTU_19  OTU_43  OTU_26  OTU_42  OTU_56  OTU_50  OTU_89  OTU_415  OTU_36  OTU_48  OTU_45  OTU_61  OTU_30  OTU_40  OTU_83  OTU_65  OTU_73  OTU_82  OTU_74  OTU_64  OTU_57  OTU_80  OTU_68  OTU_90  OTU_55  OTU_54  OTU_44  OTU_47  OTU_72  OTU_100  OTU_34  OTU_92  OTU_32  OTU_37  OTU_38  OTU_69  OTU_31  OTU_67  OTU_63  OTU_39  OTU_96  OTU_77  OTU_62  OTU_41  OTU_81  OTU_49  OTU_76  OTU_102  OTU_60  OTU_645  OTU_70  OTU_582  OTU_66  OTU_91  OTU_104  OTU_84  OTU_58  OTU_51  OTU_75  OTU_194  OTU_78  OTU_182  OTU_59  OTU_52  OTU_79  OTU_46  OTU_71  OTU_695  OTU_53   Evidence-Based Complementary and Alternative Medicine significant difference between the FBC1 group, the FBP group, and the FBPL group. Based on Fisher's exact test MetaStat software, the statistical analysis of species differences was performed. e P value of the multiple-comparison test was corrected by FDR to obtain the Q value. Finally, the species with significant differences between different groups were selected according to P value and Q value. As can be seen from Table 4, at the genus level, the abundance of Romboutsia in the FBL group was significantly lower than that in the other three groups (P < 0.05; Q < 0.05), while there were no significant differences between the FBC1, FBP, and FBPL group. e results showed that LPS caused a decrease in Romboutsia in the intestines of goslings, while the abundance of Romboutsia in the FBPL group was close to that of healthy goslings and remained relatively stable.

Discussion
LPS is the main component of the cell wall of Gram-negative bacteria and plays an important role in mediating the intestinal inflammatory response of inflammatory bowel disease, so it is often used to construct enteritis models for more in-depth scientific research. Research points out that the mechanism of LPS-induced damage to the body has the following two types [22][23][24]. e first is to act on target cells such as immune cells and epithelial cells to make surface molecules abnormal and increase cell permeability, resulting in functional damage. e second is by activating immune cells to secrete large amounts of inflammatory mediators and cause inflammation. e experiments in this paper found that the duodenum, jejunum, and ileum tissue damage caused by LPS of goslings was obvious; the levels of endotoxin, CRP, and proinflammatory factors (IL-1β, IL-6, TNF-α) in serum were significantly increased; the number of IgA-secreting cells increased dramatically; the expression of TLR4 increased; and the expression of tight junction occludin and ZO-1 decreased significantly. In addition, LPS caused an imbalance in intestinal microbial species composition, species abundance, and diversity. Combining the changes of the above indicators, we find that that goslings have enteritis; that is, the LPS-induced enteritis model is successfully constructed. e intestine not only has the functions of digestion, absorption, and secretion, but also has an important barrier function and is the main part of the body's mucosal immune system. e mucosal barrier of the body includes mechanical barrier, chemical barrier, immune barrier, and biological barrier [25]. Mechanical barrier refers to the tight connection between intestinal mucosa epithelium and epithelial cells with good morphological structure. Chemical barrier refers to mucus and bacteriostatic substances in the intestinal cavity. Immune barrier refers to sIgA in intestinal mucosa lymphoid tissue and intestinal cavity. e biological barrier refers to the intestinal probiotics that have the function of resisting harmful foreign bacteria. e (d) mechanical barrier, chemical barrier, immune barrier, and biological barrier do not operate independently but coordinate and cooperate with each other to jointly maintain the normal intestinal barrier function of the intestine, so that the entire intestinal mucosal immunity is in a healthy and stable response state [26]. is study found that PAMK increases the ratio of intestinal V/C ratio, that is, increases the surface area of intestinal villi and increases the absorption function of goslings, indicating that PAMK can promote the development of small intestine villi. In addition, PAMK can alleviate the decrease in the V/C ratio caused by LPS, indicating that PAMK has a protective effect on the development and morphological structure of the small intestinal mucosa. e normality of the morphological structure of the small intestine mucosa is directly related to the function of the intestinal barrier and reflects the level of intestinal mucosal immunity. Observing the small intestine of goslings in this study, we found that LPS has obvious damage to the small intestine of healthy goslings. e overall performance is the   loss of villi epithelial damage, cellular vacuolation, increased compensation for goblet cells, loose tissue, and obvious inflammation. Goslings show the characteristics of enteritis, and the mechanical barrier of the small intestine is damaged. We found that LPS has the most significant damage to the duodenum and less damage to the jejunum segment, which may be directly related to the high expression of TLR4 in the duodenal tissue of goslings. PAMK has a good repair effect on the damaged small intestine mucosa, of which the repair of the ileum segment is the most significant, and can promote the development of the ileum villi of healthy goslings. erefore, it can be concluded that PAMK has a certain promoting effect on the development of the small intestine. It can effectively alleviate tissue inflammation and mechanical barrier damage caused by LPS. is effect is most obvious in the ileal segment, and the mechanism of action can be further explored. Intestinal epithelial cells can maintain the permeability of small intestinal epithelium by controlling the opening and closing of tight junctions. erefore, tight junctions have an important role in preventing pathogenic bacteria and harmful antigens from entering the intestinal mucosa lamina propria and are the structural basis for maintaining the mechanical barrier function of the intestinal epithelium [26,27]. LPS, at physiological concentrations, causes an increase in intestinal tight junction permeability (TJP) via TLR4-dependent activation of the FAK/MyD88/IL-1R-associated kinase 4 signaling pathway [28]. Further research found that LPS downregulated the expression of occludin and ZO-1 mRNAs, but Astragalus polysaccharide reduces inflammatory response by upregulating the mRNA expression of ZO-1 and occludin and decreasing permeability of LPS-infected Caco-2 cells [29,30].
is study found that PAMK can not only promote the expression of occludin and ZO-1 in the duodenum, jejunum, and ileum of goslings, but also alleviate the inhibitory effect of LPS on occludin and ZO-1, so that the expression of occludin and ZO-1 in each small intestine segment is close to or higher than that of normal goslings, which has a protective effect on the tight connection of goslings. In summary, PAMK can alleviate the inflammatory response of LPS, maintain the normal intestinal mechanical barrier function, and ultimately protect the immune function of goose intestinal mucosa. e report pointed out that PAMK can cause the increase of cellular polyamine content, stimulate the migration of intestinal epithelial cells, and accelerate intestinal injury and healing, which may be the mechanism of PAMK protection and repair of mechanical barriers [14]. In addition, the effect of PAMK on the expression of occludin and ZO-1 was most obvious in the jejunum segment, while the protective effect of PAMK on the morphology of the jejunum segment was the least. We speculate that PAMK protects the goose intestinal mechanical barrier through the coordination and complementation of intestinal morphology and tight connection. When the tissue structure of a certain small intestine is damaged, the body can enhance the function of tight junctions to maintain a mechanical barrier, but the specific mechanism of action still needs more in-depth research.
Plasma cells differentiated from intestinal B lymphocytes undergo a process of polymorphism change and class conversion at the center of intestinal lymphocytic vesicles and then convert Ig type cells into mucosa-related IgAsecreting cells. erefore, the content of sIgA in the intestine is directly related to the number and secretion capacity of IgA-secreting cells, and its secretion is also regulated by the level of receptor humoral immunity. Intestinal sIgA is an important marker of intestinal acquired immunity, which can prevent bacteria from adhering to the surface of intestinal epithelial cells, neutralize toxins, and viruses and have an immunoprotective effect [25]. erefore, the maintenance of intestinal homeostasis depends largely on the role of sIgA [31]. e current research proves that the traditional Chinese medicine polysaccharides such as Astragalus polysaccharide, epimedium polysaccharide, and PAMK can promote intestinal secretion of sIgA [32]. is study also found that PAMK can increase the number of IgA-secreting cells in the duodenum and jejunum of healthy goslings. It is speculated that PAMK mainly increases the secretion of sIgA in the duodenum and jejunum. e secretion of SIgA is related to intestinal epithelial and lamina propria immune cells. ese immune cells contain antigen recognition receptors, TLRs. By recognizing these receptors, they can mediate B cell differentiation and eventually produce noninflammatory immunoglobulin sIgA. erefore, the effect of Atractylodes macrocephala polysaccharides on the number of IgA-secreting cells in different segments of the small intestine is different, which may be related to the inconsistent expression of TLR receptors in various intestinal segments. In addition, after LPS treatment, sIgA secretion in goose duodenum, jejunum, and ileum was the highest among the four treatment groups. is study also found that, after LPS treatment, the expression of TLR4 in the duodenum, jejunum, and ileum of goslings was also the highest among the four groups, and the order of TLR4 expression from high to low was duodenum, jejunum, and ileum. erefore, we explain that the increase in IgA-secreting cells after LPS treatment indirectly leads to an increase in sIgA secretion, which is related to the expression level of TLR4 receptor. LPS as a ligand, after binding to TLR4, mainly causes the activation of downstream inflammatory pathways. erefore, it is speculated that the increase of IgA secretory cells in goslings caused by LPS is a  result of inflammatory response, and PAMK has the effect of reducing the number of IgA secretory cells. is study also found that PAMK can reduce the endotoxin content in the serum of healthy goslings and also make the inflammation indicators (endotoxin, CRP, IL-1β, IL-6, TNF-α) lower. In summary, PAMK reduces the number of IgA-secreting cells in various segments of gosling intestine and inflammation indicators in serum, indicating that PAMK has a mitigating effect on the inflammation caused by LPS and has a significant role in maintaining the intestinal immune barrier function. e stability of the biological barrier function is not the absolute increase or decrease of the bacterial flora, but the mutual inhibition of different bacterial flora to achieve the relative balance of various intestinal bacteria, thereby effectively maintaining the microecological balance of the intestine and promoting the mucosal immune system stable.
e two most important ways for polysaccharides of traditional Chinese medicine (TCM) to play a role are as follows: first, polysaccharides can be directly absorbed by the intestine into the blood circulation without being degraded or degraded; second, the sugar fragments produced by the partial degradation of sugar by the bacteria directly act on the epithelial cells [33]. erefore, many TCM polysaccharides have been proven to maintain or improve the composition of intestinal flora. For example, Polygonatum sibiricum polysaccharides can prevent type 2 diabetes by regulating intestinal microbes in rats [34]; Ganoderma lucidum polysaccharides can regulate obesity-related metabolic disorders by improving intestinal microflora imbalance [35]; and PAMK can significantly improve the intestinal microflora disorder [36]. e results of this study also show that PAMK can significantly improve the imbalance of the intestinal flora of goslings caused by LPS. LPS causes a significant reduction in the flora of the genus Romboutsia, and PAMK mainly improves the stability of the abundance of the flora of the genus Romboutsia, thereby effectively improving the imbalance of intestinal flora. In 2014, scientists first isolated and identified a new type of bacterium Romboutsia in the human colon and officially created a new genus Romboutsia that year [37]. Romboutsia is a Gram-positive bacterium, which belongs to anaerobic bacteria in the intestine and belongs to Streptococcus, which can form spores [38]. Reports showed that the dicaffeoylquinic acid of Kudingcha as a dietary polyphenol is a new type of prebiotics, which has a significant regulating effect on intestinal microflora and significantly increases the abundance of Romboutsia [39]. Besides, Chen et al. detected the dynamics of the cecal microbial community in AA broilers challenged with Eimeria tenella and found that microbial shifts occur during the infection and Romboutsia decreased in abundance [40]. is shows that Romboutsia is a probiotic in the intestine, and increasing its abundance is beneficial to the body. e present study indicates that oral administration of YM001 altered the diversity and composition of intestinal microbiota in tilapia, such as Romboutsia, but these changes were only temporary, nonlethal, and recoverable [41]. erefore, the effect of PAMK on the stability of the flora may be accomplished by regulating the abundance of Romboutsia. However, the specific mode of action of PAMK to adjust the abundance of Romboutsia, the mutual adjustment relationship between Romboutsia and other flora, and what common diseases Romboutsia is related to need further research.

Conclusions
e study found that PAMK relieves LPS-induced gosling enteritis by maintaining the small intestine morphology, cytokine, tight junctions, and immunoglobulin relatively stable and improving the disorder of intestinal flora.

Data Availability
All the data supporting the findings of this study are adequately included within the article.

Conflicts of Interest
e authors declare that there are no conflicts of interest regarding the publication of this paper.

Authors' Contributions
Wanyan Li and Xuelian Xiang contributed equally to this work.