Current Insights into Phytochemistry, Nutritional, and Pharmacological Properties of Prosopis Plants

Prosopis is a regional cash crop that is widely grown in arid, semiarid, tropical, and subtropical areas. Compared with other legume plants, Prosopis is underutilized and has great potentialities. Prosopis not only is a good source of timber, construction, fencing material, and gum, but also can be applied for food, beverage, feed, and medicine. Prosopis contains numerous phytochemical constituents, including carbohydrates, proteins, fatty acids, minerals, and vitamins, while varieties of phenolic compounds have also been identified from different parts of Prosopis. Flavonoids (especially C-glycosyl flavonoids), tannins, catechin, 4′-O-methyl-gallocatechin, mesquitol, and quercetin O-glycosides are significant phenolic contents in Prosopis. Various extracts of Prosopis displayed a wide range of biological properties, such as antioxidant, antihyperglycemic, antibacterial, anthelmintic, antitumor, and anticancer. Additionally, Prosopis has the potential to be an ideal diet that contains abundant dietary fiber, minerals, galactomannans, and low-fat content. However, the bioactivity and pharmacological properties associated with Prosopis were influenced by the bioavailability of phytochemicals, various antinutritional compounds, and the interactions of protein and phenolic compounds. The bioavailability of Prosopis is mainly affected by phenolic contents, especially catechin. The antinutritional compounds negatively affect the nutritional qualities of Prosopis, which can be prevented by heating. The protein-phenolic compound interactions can help the human body to absorb quercetin from Prosopis. This literature review aimed to provide systematic information on the physical, biochemical, pharmacological, and nutritional properties and potential applications of Prosopis.

while it can also be grown with other crop species, such as millet crop in India [6]. e leaves of Prosopis cineraria are glabrous or puberulous and deciduous, with a length of 2-7 cm [7]. e fruits are elongate, slender, and 10-21 cm long, while the peel is brittle and thin [7]. e pods of Prosopis cineraria consist of about 70% of the pericarp and 30% of the seeds, while the seeds are brown in color and ovate in shape [8]. e appearance of the Prosopis cineraria tree, leaves, flowers, and pods are demonstrated in Figure 1. Prosopis can be consumed as beverages, flour, sweets, jams, bread, cakes, cookies, and syrup [9]. e Prosopis flour has brown color and sweet flavor, with a similar aroma to coffee, cocoa, coconut, or caramel [10]. Furthermore, the Prosopis gum, which is exuded from the bark of the Prosopis tree, can be used as an emulsifier, film-forming agent, foaming agent, tablet binder, and stabilizer [5]. Prosopis also provides fruit, firewood, timber, livestock feed, vegetable, construction and fencing material, medicine, and shade [11]. Prosopis can be applied as a folk medicine for different diseases, while the decoction from its twigs and flowers has antidiabetic capacity. For example, leaf extracts of Prosopis had antibacterial, antihyperglycemic, antihyperlipidemic, and antioxidant properties [12]. Overall, as all the parts of the tree are useful, Prosopis is called kalpataru in India, which means the "wonder tree" and the "king of the desert" [11].

Prosopis Cultivation and Applications
Prosopis trees are widely distributed in the regions that have the features of an extremely arid climate, low and erratic rainfall, dry atmosphere, and high wind velocities [11]. Prosopis is suitable to grow in arid and semiarid conditions of the desert because it can absorb water from the groundwater resources at a length of about 20 meters by its well-developed and expansive tap root system [13]. Also, Prosopis plays a significant role in desert ecosystems in that it can be a biomass producer and can enrich desert soil, fix atmospheric nitrogen, and increase the rate of greening. It not only can promote ecological stability of the region, but also is a vital resource for human beings, livestock, and soils [14]. Prosopis can tolerate extreme temperatures, alkalinity, and salinity, while it does not require annual plantings and can grow with other crop species, such as millet crop in India. However, the economic importance of Prosopis in the global market is limited, largely due to the ignorance of researchers and industry. e reason is that the Prosopis is presently only a regional cash crop and is not as important at the global level [15].
A Prosopis tree can yield approximately 20 to 50 kg·yr −1 of pods. If the Prosopis tree is cultivated as an orchard crop, the production can range from 1 to 8 t·ha −1 ·yr −1 of pods, while the production of Prosopis can yield more than 10 t·ha −1 yr −1 [16]. Furthermore, Prosopis gum is mainly produced in Mexico [5].
e main source of Prosopis velutina gum is the desert plains of the northwestern state of Sonora in Mexico, while the Prosopis laevigata gum is mainly collected from the lowlands of the northeastern state of San Luis Potosi (Mudgil and Barak, 2020) [5]. In Sonora, Prosopis gum is known as chucata, while its production starts in May to July and ends in July to August. Prosopis gum is collected by hand in a low humid environment, high temperature, and spiny vegetation. It is reported that the average tree density in the central region of Sonora is more than 80 trees/ha, while only approximately 8 trees/ha are gum producers [5].
Prosopis wood is suitable to make furniture and parquet flooring, due to its hardness, durableness, and appealing color. Prosopis wood can be also applied for construction, charcoal production, and mulch in gardens [3]. In addition, the bark of Prosopis is used as an astringent, tonic, and blood purifier and is said to be a potent drug for several ailments such as leprosy, dysentery, bronchitis, asthma, leucoderma, piles, muscular tremors, asthma, rheumatism, and inflammations [17]. e traditional medicines in India use Prosopis wood ash to treat gastrointestinal, respiratory, and cardiovascular diseases [11].
Prosopis leaves have antioxidant and hypoglycemic capacity due to their sterols contents, including campesterol, stigmasterol, and octacosanol [18]. e leaves contain an amount of protein, carbohydrates, and mineral matter and have high nutritive value [17]. e methanol leaf extract of Prosopis not only can reduce tumor growth, but also present in vitro and in vivo anticancer ability [19]. e smoke of the Prosopis leaves can be applied for treating eye sickness [20]. Leaf paste can be used to treat boils and blisters, such as mouth ulcers in livestock and leaf infusion on open sores on the skin [17]. Furthermore, Prosopis leaves can be the feed for camels, goats, and donkeys [11].
As an exudate from the tree rind, the Prosopis gum has several characteristics including high solubility, emulsifying, and high viscosity, which are similar to Arabic gum, and can act as a kind of stabilizer [21]. First, Prosopis gum can contribute to preparing emulsion and improving the stability of the emulsion, and therefore, Prosopis gum can function as an emulsifier [5]. e properties of Prosopis gum are similar to Arabic gum that can contribute to the formation and stabilization of oil-in-water emulsion and exhibit potential for the encapsulation of orange citrus oil for the improvement of spray-dried products. However, compared with Arabic gum, Prosopis gum is a better emulsion stabilizer because it has unique advantages in providing more stability and lower mean size of oil droplets [22]. Second, Prosopis gum can be used as a replacer of Arabic gum to encapsulate the food to maintain colors, aroma, and flavors through spray drying [5]. Prosopis gum not only can encapsulate alone but also can be used in association with other encapsulating agents to encapsulate essential oils.
ird, Prosopis gum is used as a foaming agent due to its foaming properties [5]. Forth, the addition of Prosopis gum can increase the shelf life of food [5]. For example, because of the chemical composition, Prosopis gum can be added to bread to increase the shelf life to 10 days. Finally, edible films made by Prosopis gum are excellent barriers to prevent oxygen, water vapor, and CO 2 so that they can develop storage stability and extend the shelf life of fruits and vegetables [23].
Prosopis pods are comprised of approximately 72% pericarp and 28% seeds. e seeds of Prosopis pods consist of 2 Evidence-Based Complementary and Alternative Medicine episperm, endosperm, and cotyledons, while the pericarp is constituted by epicarp, mesocarp, and endocarp [24]. Carbohydrates (including fiber and soluble sugars), proteins, and bioactive compounds, such as tannins, steroids, flavones, and alkaloids, are the main components of Prosopis pods, which provide Prosopis pods with antioxidant, anti-inflammatory, and antihypertensive functions [10].
With the advantages of rich energy and high nutritional values, the Prosopis pods are a good source of food for human beings [11]. Prosopis pods are considered delicious, fragrant, and sweet [9]. is is the main reason that Prosopis pods can be used in a variety of foods such as bread, cakes, cookies, jams, syrup, beverages, fermented beverages, "atoles" (Mexican beverage), cheese, and a coffee or chocolate substitute [9,25]. Besides, in South America, "añapa," which is a kind of sweet, non-fermented, and nonalcoholic beverage, and "aloja," which is a fermented beverage with alcohol, are two popular food products produced by Prosopis pods [26]. People in South America also grind, roast Prosopis pods, or mix with milk to produce flour, cakes, and syrup [26]. Prosopis pods are also used to make a dessert called "bolanchao," which is popular in Argentina [24]. e syrup derived from the Prosopis pods is also commercialized in South American countries, such as Peru, Ecuador, and Chile, for making confectionery and cocktails [27]. e water extraction of the sugars from the Prosopis pods can be used to make a beverage called "yupisín," while "algarrobina" is the syrup boiled from Prosopis pods [16]. Furthermore, it is popular to use dried Prosopis as animal feed [17]. With the right proportion, Prosopis pods can be effectively used in the animal feed industry [28]. Without affecting product quality, feed intake, feed conversion, and body weight gain of Omani goats can be increased by the addition of 20% or 200 g/kg P. juliflora pods in feed [28]. Furthermore, in Ethiopia, 10 and 20% inclusion of ground P. juliflora pods were found to reduce feed production costs without influencing biological performance [28]. Prosopis flour (PF) has brown color and sweet taste, while it has roughly the same energy and protein content as wheat flour. Furthermore, this gluten-free flour also has flavors of coffee, cocoa, coconut, caramel or molasses, cinnamon, and hazelnut [9,29]. e Prosopis flour, which is called "mesquite flour" or "algarroba flour," is made by the whole ripe Prosopis pods [10]. It has been studied that Prosopis flour can be applied for composite bread and other cereal-based formulations because Prosopis flour is rich in iron, potassium, fiber, and calcium, and the amino acids in Prosopis flour complement cereal proteins [10,30].
e Prosopis flour levels are limited in bread formulations by the lack of starch. e mixture of 5-25% Prosopis flour and wheat flour can develop the taste of food products; 5% Prosopis flour is suitable to add in sweet bread to gain acceptable taste and texture, while 25% Prosopis flour is applied for producing biscuits, which can reduce the addition of sugar [16]. e distinct viscoelastic characteristics of wheat dough of Prosopis are related to the gluten network [9]. e addition of Prosopis flour makes a negative influence on the volume, texture, and structure of bread since it cannot only disturb the gluten network but also result in network deficiency and different rheological behavior due to the existence of the globular proteins and fiber in Prosopis flour. Besides, there are technical problems such as shredded crumbs and low volume that are caused by the gluten-free properties of Prosopis flour. Furthermore, with the content of Prosopis flour increased, the maximum volume, specific volume, and compact crumb would decrease, while the fermentation times would increase. As a result, the major difficulties to add Prosopis into baked food are the rheological changes in the dough and the final product [9].

Phytochemicals in Prosopis
e main nutrient components of Prosopis are shown in Table 1. e methods and technologies, which were used to determine the major chemical compounds in Prosopis, are demonstrated in Table 2.
e identification of phytochemicals in Prosopis is summarized and displayed in Table 3.

Tree
Leaves Flowers Pods  e main components of carbohydrates in Prosopis are galactose and mannose, while Prosopis also contains minor amounts of glucose and arabinose, which might be caused by a complex polysaccharide composition or contaminants proceeding from the seed coat of Prosopis. In Prosopis flexuosa seed endosperm, galactomannan is the major polysaccharide (about 85% w/w of galactose plus mannose) [32].
Prosopis pods contain varieties of macronutrients, while different parts of Prosopis pods have different macronutrient contents. e carbohydrate content of cotyledon flour of Prosopis alba pods was 8.97 ± 0.05 g/100 pod flour, which was significantly lower than that of mesocarp pod flour (52.08 ± 0.09 g/100) [1]. Besides, the content of soluble reducing sugar in cotyledon flour (0.21 ± 0.07 g/100g) was also lower than that of mesocarp flour (3.73 g/100 g pod flour), while the fat content in cotyledon flour was higher with the value of 12.20 ± 0.05 g/100 g flour [1]. e main content in cotyledon flour of P. alba pods was crude protein (62.09%), and by comparison, the crude protein in mesocarp flour was only lower than 4% [1]. Furthermore, the protein content in cotyledon flour of P. alba pods was significantly higher than that in soybeans (34.6%), lentils (25.4%), peas (22.9%), and chickpeas (18.5%) [31]. e main protein varieties in cotyledon flour of P. alba pods were albumin (44.59 ± 1.70%) and globulin (30.82 ± 1.64%) [31]. Overall, with the advantages of high protein content with biological capacity, low carbohydrate content, and low-fat content, the cotyledon flour of Prosopis alba can be applied for producing foods or food supplements with low calories, which can be also along with the addition of cereal proteins (such as cookies, cereal bars, and coffee substitutes) [33].
Prosopis gum is a complex carbohydrate that consists of D-galactose, L-arabinose, D-glucuronate, D-mannose, and D-xylose, but it does not contain L-rhamnose, which makes it different from Arabic gum in structure [5]. In addition to carbohydrates, Prosopis gum also contains about 3% protein [34]. However, mesquite gum obtained from Prosopis alba gum contains more than 13% protein, which is much more than that of other Prosopis [35]. Serine, hydroxyproline, valine, and glycine are the main amino acids of protein in Prosopis gum [36]. It is reported that the emulsification ability of Prosopis gum is chiefly related to the protein content, and therefore, Prosopis alba gum has a stronger emulsification ability [37]. Fatty acids including palmitic, stearic, and behenic acid are also significant contents in the lipid portion of Prosopis gum [5].

Amino Acids.
Animal proteins are usually complete proteins with complete essential amino acids, and most plant proteins (including cereal proteins) are incomplete proteins, which lack one or more essential amino acids [31]. However, Prosopis flour contained all essential amino acids. Several essential amino acids, such as isoleucine and valine, which were deficient in other grains, were rich in concentration in Prosopis flour [31]. Prosopis flour also contains amino acids with sulfur (cys 1.47%), which is also limited in other grains [31]. e previous study demonstrated that the dry matter of P. juliflora pods consists of 0.99% aspartic acid, 0.28% threonine, 0.14% cystine, 0.43% valine, 0.10% methionine, 0.27% isoleucine, 0.52% leucine, 0.29% tyrosine, 0.33% phenylalanine, 0.37% alanine, 0.19% histidine, 0.32% lysine, 0.56% arginine, 0.41% serine, 1.4% glutamic acid, and 0.51% glycine (Rani et al., 2013). Based on the previous study, the main amino acids in Prosopis were Asp and Glu, which took up about 30% of the total amino acids with the value of approximately 22 mg/100 g protein [38]. e content of another amino acid, Leu, was about 6.5 mg/100 g protein [38]. Besides, the total essential amino acids (TEAAs) take up more than 45% with the value of 34 mg/100 g protein in Prosopis alba, which is similar to the values of two oil seeds,

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Vigna subterranean and Phaseolus coccineus [39,40]. e nutritional value of a protein depends on the nitrogen content and essential amino acids, and thus, Prosopis has the potential to provide essential amino acids to people as a diet [38]. e hydrophobic region of the protein in Prosopis was comprised of 6.5 g/100 g protein for an essential aliphatic amino acid (EAAA), which meant that Prosopis had good emulsification capacity [38]. Furthermore, the content of the total acidic amino acid (TEAA) (31.3 g%) was higher than the total basic amino acid (TBAA) (15.7 g%), which demonstrated that the protein in Prosopis is acidic [38].

Fatty Acids.
e most abundant fatty acid in Prosopis was palmitic acid, which was followed by oleic acids [10]. However, Prosopis only contained very little caprylic acid, capric acid, lauric acid, and pentadecanoic acid. In the previous study, nine fatty acids in cotyledon flour of Prosopis alba were analyzed, which included saturated fatty acids (SFAs), monounsaturated (MUFAs), and polyunsaturated fatty acids (PUFAs) [31]. As a result, PUFA (linoleic acid, 60%) was the major total fatty acid (TFA) in Prosopis alba, which was followed by MUFA (oleic acid, 18%) and SFA (palmitic acid, 16%). As an essential fatty acid, linoleic acid (omega-6 fatty acids) cannot be synthesized by humans and other animals, which can be only gained from food sources [31]. Linoleic acids play an important role in people's health. First, linoleic acids can regulate renal and pulmonary function, vascular tone, and inflammatory responses by functioning as structural components of membranes and precursors of eicosanoids [5]. Second, linoleic acid not only can induce the expression of uncoupling proteins and coordinately up modulate different dozen genes related to oxidative energy metabolism but also can motivate mitochondrial biogenesis and increase energy reserves, which causes stabilized synaptic properties [41]. erefore, Prosopis has the potential to be a good source of essential fatty acids. Furthermore, it was also reported that Prosopis flour can be also applied for composite bread to increase the content of healthy unsaturated fatty acids. Additionally, the fatty acid profile in bread was not influenced by the heating process [42].

Fiber.
Prosopis alba flour contained more than 9% insoluble fiber, so it can be a good source of fiber [31]. Cellulose, hemicellulose, lignin, extractives, ash, and water are the main content of woody biomass. Based on a previous study, Prosopis juliflora contains 25%-30% hemicellulose, 40%-45% cellulose, 11%-28% lignin, and 3%-15% extractives [43]. Prosopis gum is a great source of dietary fiber due to its low viscosity, which is widely used in producing fiberfortified food products [5]. e low viscosity of complex carbohydrates is very much suitable for its use as a dietary fiber source. Dietary fibers are complex carbohydrates that are resistant to enzymatic secretion of the human gastrointestinal tract and are beneficial for human health [16].
In the cotyledon flour of Prosopis alba, C-glycosyl flavonoids were the major free phenolic compounds, which were followed by tannins [4]. Flavonoids are a significant type of polyphenols that commonly exist in vegetables and contribute to maintaining human health, and therefore, they have attracted more attention than before [46,47]. e previous study showed that flavonoids could be the inhibitors of glycohydrolases. e high galactomannan content may positively influence the accumulation of C-glycosidic flavones in Prosopis seeds [48]. Apigenin C-glycosides are stronger a-glucosidase inhibitors than acarbose, and therefore, C-glycosides are potential to apply for delaying the absorption of carbohydrates [26]. Besides, tannins are another important polyphenolic class because of their structural diversity and bioactivity [49]. Flavonoid content was also high in the mesocarp flour, while cotyledon flour of Prosopis alba did not contain hydrolysable tannins and anthocyanins [31]. Polar extracts from P. juliflora wood and bark contained several flavanols, including catechin, 4′-O-methyl-gallocatechin, and mesquitol [50]. Quercetin Oglycosides, apigenin-based C-glycosides, flavonoid C-glycosides, and O-glycosides are also important phenolic compounds in Prosopis, which present anti-inflammatory, antioxidant, and antiplatelet properties [4,46]. e structures of some typical phenolic compounds mentioned above are shown in Figure 2. By balancing antioxidants and oxidants in the human body, phenolic compounds can control oxidative stress and prevent chronic illnesses, such as cancer, atherosclerosis, or cardiovascular disease [45].

Carotenoids and Ascorbic
Acid. Both carotenoids, which can be precursors of vitamin A, and ascorbic acid are good antioxidants. Furthermore, carotenoids and ascorbic acid can delay the formation of off-flavors and rancidity compounds to extend the shelf life of the product, so they are applied for natural food or beverage preservatives [31]. In Prosopis flour, the carotenoid content was 10.55 ± 0.05 mg-CE/100 g flour, which was significantly higher than ascorbic acid (0.33 mg L-AA/100 g flour) [31]. As essential structural contents of the photosynthetic and reaction center, carotenoids can protect photosynthetic organisms to avoid potentially harmful photooxidative processes [51]. Besides, carotenoids are a class of natural fat-soluble pigments found principally in plants, algae, and photosynthetic bacteria, where they contribute to the photosynthetic process. Furthermore, carotenoids can also act as accessory pigments and protect chlorophyll from photooxidative destruction [52].

The Pharmacological Value of Prosopis
Prosopis is widely used as a folk medicine for a variety of ailments in South America [7]. Prosopis flowers can be applied to safeguard against miscarriage during pregnancy by pounded and mixed with sugar [17]. e Prosopis flowers with twigs can be used as an antidiabetic agent [7]. With the features of dry, acrid, and bitter with a sharp taste, the bark of Prosopis cineraria trees can be applied for treating leprosy, dysentery, rheumatism, bronchitis, asthma, cough colds, leucoderma, muscle tremors, and piles as an internal drug [17]. It was also reported that the bark of Prosopis cineraria trees has anti-inflammatory activity and can cure scorpion sting [17]. e smoke of Prosopis leaves can be used for treating eye infections [7]. e dry pods of Prosopis also contribute to avoiding protein-calorie malnutrition and iron calcium deficiency in the blood [7]. Different extracts of Prosopis demonstrate various pharmacological activities, such as analgesic, antipyretic, antihyperglycemic, antioxidant, antihypercholesterolemic, and antitumor, which will be discussed in the following paragraphs. e bioactivities of phytochemical compounds isolated from Prosopis are displayed in Table 4.

Antioxidant
Activity. DPPH, ABTS, hydroxyl, superoxide, nitric oxide, and hydrogen peroxide were used to estimate the antioxidant potential of different leaf solvents of Prosopis laevigata in a previous study [11].
e results

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demonstrated that the content in Prosopis laevigata leaves can provide hydrogen and remove an odd electron from a free radical to avoid radical reactivity. Besides, methanolic and ethyl acetate extracts have the strongest scavenging capacity [11]. According to ABTS radical assay, n-hexane, methylene chloride, ethyl acetate, and n-butanol extracts of Prosopis farcta aerial parts presented 83.1, 82.0, 87.2, and 87.0% inhibition percentages (I%), respectively, compared with ascorbic acid (89.2%) [54]. e extract of P. farcta fruit also showed similar results to P. farcta aerial parts, while the antioxidant capacity was directly related to the high phenol and flavonoid contents [55]. Flavonoids are important natural antioxidants that can be obtained from Prosopis [56].
In both in vitro and in vivo conditions, the extracts of Prosopis were detected to have antioxidant capacity due to the content of flavonoids, while the antioxidant properties in vitro were higher than those in vivo. However, if the state of oxidation is not induced, a high concentration of flavonoids in the extract of Prosopis can have a prooxidant effect in an in vivo environment [43]. Additionally, the methanolic extract of the Prosopis farcta stem bark also had antioxidant activity [57]. e methyl linoleate oxidation inhibition test was also applied for evaluating the antioxidant capacity of Prosopis juliflora extract [43].   8 Evidence-Based Complementary and Alternative Medicine Evidence-Based Complementary and Alternative Medicine extract of Prosopis juliflora was stronger than that of butylated hydroxytoluene (the reference antioxidant), probucol, and a-tocopherol [58]. Overall, Prosopis has the potential to be a source of natural antioxidants for food supplements or pharmaceutical industry formulations and can be used in treating inflammatory diseases, cancer, and diabetes [59].

Antihyperglycemic Activity.
Alloxan-induced hyperglycemia model was applied for evaluating the antihyperglycemic capacity of 50% hydroalcoholic extract of stem bark of Prosopis cineraria [11]. A group of hyperglycemic mice was given 300 mg/kg B W once a day for 1.5 months. As a result, the body weight and fasting blood glucose level of those mice were decreased, while liver glycogen content was increased compared with the control group. Furthermore, the Prosopis could also increase the activity of antioxidant enzymes and the concentration of nonenzymatic antioxidants to reduce the oxidative damage in the tissues of hyperglycemic mice [60]. erefore, the Prosopis cineraria extract has antidiabetic and antioxidant potential.

Analgesic and Antipyretic
Activity. Using Brewer's yeastinduced hyperpyrexia model, the petroleum ether extract of stem bark of Prosopis cineraria showed antipyretic capacity in experimental rats [7]. e ethanolic extract of the root of Prosopis cineraria was determined by tail immersion and hot plate method [61]. In addition, an acetic acid-induced writhing test model was applied for evaluating the analgesic activity of the aqueous extract of Prosopis cineraria leaves [7]. As a result, the dose of 200 mg/Kg B W can develop analgesic activity in mice [61]. Furthermore, through Brewer's yeast-induced hyperpyrexia model, the antipyretic activity was also developed at the same dose [11].

Antitumor Activity.
Hydroalcoholic extract of Prosopis cineraria leaves and bark demonstrated antitumor properties against the Ehrlich ascites carcinoma tumor model [7]. Also, the protective action against induced experimental liver tumors of methanolic extract of Prosopis cineraria leaves was estimated in male Wistar rats. e administration of the extract (200 and 400 mg/kg) can decrease the levels of mitochondrial lipid peroxidation (LPO) and liver weight in a dose-dependent manner, while the levels of mitochondrial enzymatic antioxidant can also be reduced by the extract [7].

Respiratory and Gastrointestinal Activity.
e spasmolytic, bronchodilator, and vasodilator properties of methanolic extract from the stem bark of Prosopis cineraria were determined in a previous study [7]. As the result, blockade of Ca 2+ channels may mediate the bronchodilator and vasodilator activities.

Anticonvulsant Activity.
rough inducing convulsions in mice, pentylenetetrazole (PTZ) and maximal electroshock (MES) can be used to estimate the anticonvulsant activity of methanolic extract of Prosopis cineraria [11]. As a result, methanolic extract of stem barks of Prosopis cineraria had an anticonvulsant capacity, while the hind limb tonic extensions (HLTEs) induced by MES can be inhibited and the Extract against MCF-7 cell line (IC 50 � 5.6 μg/mL) and HeLa cell line (IC 50 � 6.9 μg/mL). [57] drug against PTZ-induced seizures can be protected by the Prosopis extract. e duration of convulsions can be decreased by the mixture of methanolic extract of P. cineraria at doses of 200 and 400 mg/kg and phenytoin at 25 mg/kg. Overall, the inhibition of MES-induced seizures and the protector effect of drugs against PTZ-induced seizures can play important role in myoclonic control and the absence of seizure protection to human beings [62].

Antibacterial Activity.
It is important to seek new antibacterial compounds due to microbial antibiotic resistance [3]. It is reported that juliflorine, synthesized in Prosopis, has antibacterial capacity against Corynebacterium diphtheria var. mitis, Corynebacterium hoffmanni, Bacillus subtilis, Staphylococcus aureus, and even Streptococcus pyogenes, which is resistant to most antibiotics [63]. Besides, varieties of alkaloids, tannins, phenols, flavonoids, terpenes, and steroids can be obtained from different parts of P. juliflora, which have antibacterial capacity against Gram-negative bacteria [3]. ese Gram-negative bacteria are resistant to antibiotics such as minocycline, chloramphenicol, and erythromycin.
In a previous study, the potential antibacterial capacity of aqueous and ethanolic extract of P. farcta against methicillinresistant S. aureus (MRSA) was determined. As a result, the minimum inhibitory concentration (MIC), which means the least concentration of antimicrobial agent that prevents microbial growth, of aqueous extract was 100 mg/mL, while the MIC of ethanolic extract was 25 mg/mL [64,65]. For comparison, previous research on the antimicrobial effect of tomato seeds indicated that the MIC of the two types of tomato seeds remained essentially in the range of 5-20 mg/mL when extracted with organic solvents, notably both types of tomato seeds extracted with hexane having MIC values of 5 mg/mL [66].
Another antibacterial research on brown seaweed extraction indicated the MIC of the several seaweeds analyzed was generally higher than or equal to 31.3 mg/ml for MRSA [67]. Both of the research above presented favourable results in terms of antibacterial capacity.
Agar well diffusion method can be used to estimate the antimicrobial properties of extracts of different parts (pods and steam bark) of Prosopis cineraria [11]. For Prosopis cineraria pods, methanol extract can inhibit E. coli, P. aeruginosa, S. typhi, and K. pneumoniae, but chloroform and aqueous extracts cannot inhibit those microorganisms. Besides, for Prosopis cineraria stem bark, the methanolic and aqueous extracts demonstrated moderate antibacterial capacity to E. coli, P. aeruginosa, S. typhi, and K. pneumoniae, which is related to the flavonoid and tannin content [69]. e 20% aqueous extract of Prosopis juliflora leaves also had antibacterial activity against three different phytopathogenic Xanthomonas pathovars, namely Xanthomonas axonopodis cf. phaseoli with an inhibition zone of 12.07 ± 0.14 nm, Xanthomonas axonopodis cf. malvacearum with an inhibition zone of 15.92 ± 0.07 nm, and Xanthomonas campestris cf. vesicatoria with an inhibition zone of 16.37 ± 0.12 nm [43,70]. ese pathogenic bacteria can cause common blight of beans, angular leaf spot of cotton, and bacterial spot of tomato. Furthermore, 14 human pathogenic bacteria were also inhibited by the Prosopis extract. Likewise, the Prosopis juliflora extract had an antidermatophytic capacity that could be used to inhibit Trichophyton mentagrophytes infection in rabbits [70]. As a result, 2.5% juliflorine obtained from Prosopis juliflora can cure 75% of dermatophytic lesions within 1 month.
Staphylococcus aureus (Gram-positive), Escherichia coli (Gram-negative), and Candida albicans (fungal pathogen) were prepared for determining the antimicrobial capacity of ethyl ether and alcoholic extracts of Prosopis cineraria leaves [11]. Nutrient broth (10% peptone, 0.5% labanco, 0.5% NaCl, and pH 7.5) was prepared for the growth of S. aureus and E. coli, while liquid medium (1% peptone, 4% glucose, and pH 5.8) was the growth medium for C. albicans. By comparing with standard antibiotics, ethyl ether and alcoholic extracts can inhibit the growth of S. aureus (0.80 and 0.74 I/ C a , respectively), E. coli (0.97 and 0.89 I/C a , respectively), and C. albicans (0.62 and 0.86 I/m a , respectively), where I means inhibition zone, a means ratio of diameters of the inhibition zone to extracts (10 µg) under observation (I) and diameter of inhibition zone due to standard reference antibiotics, C means chloramphenicol (30 µg) against S. aureus in 30 mm and E. coli in 32 mm, and m means Mycostatin (100 units) against C. albicans in 32 mm [71].

Hypolipidemic and Antiatherosclerotic Efficacy.
Hyperlipidemic rabbits can be used as samples to estimate the hypolipidemic and antiatherosclerotic capacity of Prosopis cineraria bark extract [11]. High-fat diet and cholesterol powder were fed to rabbits to induce exogenously Evidence-Based Complementary and Alternative Medicine hyperlipidemic. Prosopis cineraria bark extract was used to treat a group of those rabbits, while the other group was treated with a standard drug. e previous research demonstrated that the values of serum total cholesterol, LDL-C, triglyceride, VLDL-C, and ischemic indices (total cholesterol/LDL-C and LDL-C/HDL-C) were decreased after the treatment of 70% ethanol extract of Prosopis cineraria bark. erefore, Prosopis cineraria bark has the potential to be used in preventing hyperlipidemic and atherosclerotic [72].

4.9.
Anti-Inflammatory Activity. Carrageenan-and histamine-induced paw edema in rats was used to demonstrate the anti-inflammatory capacity of P. juliflora [3]. Carrageenan, which is comprised of polysaccharides and histamine, was applied in inducing inflammation in rats. e anti-inflammatory capacity of P. juliflora was demonstrated by assays of carrageenan-induced and second histamineinduced paw edema [3]. Prostaglandins can promote the second phase of inflammation in rats, and thus, the inhibition of prostaglandins is important for anti-inflammatory drugs. e previous study of SivaKumar et al. [73] displayed that methanol extracts of P. juliflora bark can block prostaglandins and inhibited carrageen-induced inflammation in rats, and thus, P. juliflora has anti-inflammatory capacity.

Antihelminthic Activity.
e different solvent extracts of Prosopis cineraria stem bark can act as efficient antihelminthic agents in a dose-dependent manner. For example, the methanolic extract of Prosopis cineraria stem bark at a dose of 160 mg/mL resulted in paralysis in 25 min and death in 62 min against Phretima posthuma, which use a similar time with the standard drug piperazine citrate (10 mg/mL) [75]. erefore, the extract of Prosopis cineraria stem bark has the potential to replace piperazine citrate in antihelminthic properties.

Apoptotic
Activity. Breast cancer cell line MCF-7 and noncancerous cell line HBL 100 were used to estimate the apoptotic capacity of methanolic extract of Prosopis cineraria leaves [11]. MCF-7 and HBL 100 were stained by different staining methods, including acridine orange/ ethidium bromide staining, propidium iodide (Pi) staining, ethidium bromide (Etbr) staining, Hoechst 33342 staining, and DAPI (4′-6′-diamidino-2-phenyl indole) staining [76]. e apoptotic ratio in MCF-7 was increased by the addition of methanolic extract of Prosopis cineraria leaves, but that in HBL 100 was not influenced. Prosopis cineraria can act as a promising chemotherapeutic agent to treat cancer because it can promote apoptosis or programmed cell death to inhibit the proliferation of cancer cells [76].

Antidepressant Activity.
e Prosopis cineraria leaf extract can act as an antidepressant agent and skeletal muscle relaxant agent, which is a traditional medicine to treat different CNS diseases [11]. Forced swim test (FST), which was applied for estimating antidepressant effect, showed that the duration of immobility time in FST was reduced by the Prosopis cineraria leaf extract at doses of 200 mg/kg [77].

Anticancer Effects.
e total alkaloid extractions from leaves of P. juliflora have a cytotoxic capacity on cancer cells, such as human T-cell leukemia cells [3]. e IC 50 values of P. juliflora extract at 24, 48, and 72 h were recorded as 90.5, 42.5, and 20.0 μg/ml/1 × 10 6 cells against cancer cells [78]. Additionally, mitotic cell divisions by chromosome aberrations can be inhibited by extracts of flowers of P. juliflora [3]. e root cells of the onion plant Allium cepa can be used to describe the antiproliferative capacity of compounds. e compounds obtained from flowers of P. juliflora can act as spindle inhibitors and cause the clastogenic effects in Allium cepa cells [3]. e anticancer properties of different extracts from Prosopis farcta aerial parts to prevent four human tumor cell lines (HepG-2, HeLa, PC3, and MCF-7) were evaluated. e n-butanol extract of Prosopis displayed strongest capacity to prevent the MCF-7 cell line (IC 50 � 5.6 μg/mL), which was comparable to 5-fluorouracil (IC 50 � 5.4 μg/mL). Besides, the ethyl acetate extract of Prosopis farcta also showed the strongest properties against HeLa cell line (IC 50 � 6.9 μg/ mL), while the capacity against 5-fluorouracil was lower (IC 50 � 4.8 μg/mL) [57].
In contrast, the hydroethanolic extracts of P. avium stems and flowers and the infusions of its flowers, which are efficient in antiproliferative, show a significant reduction in cell viability (IC 50

Bioavailability of Phytochemicals in Prosopis.
Phenolic compounds are the main contributors to the biological activity of Prosopis. e oxidation of biomolecules can be inhibited by antioxidants through the donation of hydrogen to free radicals, while this ability of different antioxidants can be determined by 2,2′-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays [4]. In a previous study, the Prosopis alba extracts rich in free flavonoids reached SC 50 values (the scavenging activity of 50% ABTS•+ and DPPH•, and the values are expressed in μg GAE/mL in free phenolic compounds or bound phenolic compounds) between 6 and 17 μg/mL for ABTS, while the results of 50% DPPH scavenging activity were 11 to 15 μg/mL [4]. By comparison, the results gained from Prosopis alba dominated by bound phenolic compounds were 2.5 and 7 μg/mL for ABTS and 1.5 and 1.7 μg/mL for DPPH [4]. As a result, free phenolic compounds were less active than bound phenolic compounds. e bound phenolic compounds in Prosopis are beneficial for human health because they can avoid the digestion in upper gastrointestinal and be absorbed into blood plasma [81]. Additionally, the free and bound phenolic content in Prosopis can protect against the peroxidation of unsaturated biomolecules such as ß-carotene and linoleic acid [4]. e free phenolic compounds of Prosopis were more active than bound phenolic compounds, which represented that free phenolic compounds are better in protection against lipoperoxidation [4]. e free radical-scavenging activity of the leaves and pods of Prosopis was also evaluated in previous research, which could represent the biological activity of Prosopis. In Prosopis tamarugo, phenethylamine was one of the main aromatic amines, while alkaloids ß-phenethylamine and tryptamine were detected in Prosopis chilensis. In addition, the total phenolic content was comprised mainly of catechin, which resulted in the observed activity [43]. e extracts of aerial parts of P. alpataco, P. Argentina, P. chilensis (Molina) Stuntz., Prosopis flexuosa DC., and Prosopis pugionata were applied for isolating the alkaloids tryptamine, phenethylamine, and piperidine in the previous study. e isolated compounds were assessed for DNA binding, ß-glucosidase inhibition, and free radical-scavenging (DPPH assay) effects. At a concentration of 0.5 mg/ mL, DNA-binding activities showed tryptamine (28%), phenethylamine (0%-27%), and piperidine derivatives (47%-54%), respectively. Tryptamine and 2-β-methyl-3β-hydroxy-6-β-piperidine dodecanol displayed a moderate inhibition (27%-32%) of the enzyme ß-glucosidase at 100 μg/mL. In the DPPH assay, catechin in Prosopis showed a free radical-scavenging capacity. HPLC was used to determine the phenolic compounds of the methanol extract of Prosopis, which showed the result that more than 70% of phenolic content in Prosopis was catechin [82]. e juliflorine from Prosopis juliflora existed acetylcholinesterase inhibitory substances [43]. Furthermore, the DPPH and HPLC assays also demonstrated that alkaloids were not the main factor to cause the free radical-scavenging effect of Prosopis extracts [82].
Similar to ABTS or DPPH methods, the OH radical inhibition is another method that is based on the singleelectron transference (SET) mechanism to evaluate the rate of the OH radical inhibition, and therefore, the antioxidant activity of Prosopis gums can be estimated [83]. Due to the higher flavonoid content, the percentage of OH radical inhibition in Prosopis alba gum is lower than that of Arabic gum. e principle is that flavonoids can inhibit OH radicals [49]. Metal-chelating activity is also an important secondary antioxidant mechanism that prevents the prooxidant effect of transition metals on the decomposition of hydroperoxides or H 2 O 2 in the radical chain reaction [84]. A previous study showed that Prosopis alba gum has a stronger ability in scavenging ferrous ions than Arabic gum chiefly due to the higher content of tannins, which can chelate with different metal ions [85]. Overall, Prosopis alba gum has a stronger antioxidant capacity than Arabic gum, and hence, the applications of Prosopis gum as a natural antioxidant are worthy to be further researched [49].
As another antioxidant characteristic, reducing power can describe how the radical reactive species are reduced by a substance in the peroxidation mechanism [86]. Except for polyphenols, reducing power is also influenced by amino acids, reducing sugars, etc. e previous study showed that the reducing power value of Arabic gum was only 1/2 of Prosopis alba gum. As the reducing sugar content of Arabic gum was higher than that of Prosopis alba gum, the reducing power of Prosopis alba gum is mainly attributed to the phenolic compounds [49].

Antinutritional Compounds.
Although Prosopis seeds have rich nutritional contents, they are not widely used as foods or feeds due to the existence of antinutritional contents [87]. e antinutritional compounds in Prosopis include total free phenolics, tannins, phytic acid, L-DOPA, trypsin inhibitor activity, and lectins, while the main antinutritional contents are total free phenolics and tannins, which can reduce protein and fiber digestion and dry matter intake [88]. e total free phenolics and tannins can be excluded by dehulling, soaking, and heat treatment or cooking process due to their water-soluble properties [33]. e antinutritional factors make a negative influence on the nutritional qualities of Prosopis [89]. In Prosopis, total free phenolic compounds took up about 4.93 to 8.58%, while tannins occurred within 6.81 to 9.15%. e seed coat of Prosopis was relatively rich in tannins and total free phenolic compounds. Besides, the protein digestibility of human beings is negatively influenced by the presence of tannins [90].
Several processing methods can avoid the negative influence of antinutritional factors on the edibility of Prosopis [91]. As a high-temperature, short, and versatile food operation, extrusion cooking technology can enhance the shelf life and nutritional properties by fully cooking the agricultural raw materials [92]. e extrusion cooking of Prosopis can avoid antinutritional factors and develop protein digestibility cost-effectively [15]. e tannin content and lectin agglutinating activity would be increased as the total phenol content and protease inhibitor activity decreased [93]. Furthermore, ruminants may be poisoned by phenolic compounds, which can not only result in lesions in their digestive tract and inhibit the production of digestive enzymes, but also inhibit their digestion of soluble carbohydrates and hemicellulose [94]. During the heat treatment, the formation of complexes between tannins and proanthocyanidins will result in condensation, which can reduce their Evidence-Based Complementary and Alternative Medicine concentration [93]. Condensed tannins can form tanninprotein complexes, which can prevent microbial degradation of plant proteins, inhibiting their entrance into the small intestine [93].
Autoclaving and dry heat treatment is an advanced technology that can significantly inhibit trypsin inhibitor activity [95].
e main storage form of phosphorus in Prosopis is phytic acid, which is a kind of antinutritive factor [96]. e previous research demonstrated that the content of phytic acid of Prosopis can be reduced significantly by pressure cooking of soaked-dehulled seeds [96]. Lectins, which are combined with the cells that line the intestinal mucosa, can result in a nonspecific interference with the absorption of available nutrients and reduce the feed intake [90]. Phyto-hemagglutinating activity of Prosopis samples was higher with "A" blood group of human erythrocytes but lower with "O" blood group of human erythrocytes, which was consistent with the previous findings of Mucuna spp. [95].

Effects of Protein-Phenolic Compound Interactions.
Polyphenols and proteins can interact with each other to form nanocomplexes [97]. Protein-polyphenol interactions are noncovalent bonding through hydrophobic, van der Waals, hydrogen bonding, and ionic interactions [98]. Depending on the different strength of the interactions, the complexes formed by polyphenols and proteins can be soluble or precipitate out of solution [99]. Temperature, the structure of the phenolic contents, the properties of the protein, and the existence of other chemical compounds can affect the formation of the protein-polyphenol complex [98]. Besides, the addition of polysaccharides, including Arabic gum, cyclodextrins, pectin, and xanthan gum, can increase solubility and avoid sedimentation of protein-polyphenol complex [98]. A previous study used fluorescence measurements to demonstrate that a pea (Pisum sativum) protein isolate would interact with quercetin via hydrophobic interactions and hydrogen bonding and the PPI-Q-MG (pea protein isolate-quercetin-mesquite gum) can develop the physical and chemical stability of quercetin [98]. As a result, the interactions of protein-phenolic compounds contribute to developing the transport systems of quercetin from Prosopis food products and beverages.

Conclusion and Future Direction
In conclusion, Prosopis are underutilized legume plants that are widely distributed in arid, semiarid, tropical, and subtropical countries. Prosopis can be used as food, beverages, firewood, timber, livestock feed, vegetable, construction and fencing material, medicine, and shade. e chemical constituent of Prosopis includes macronutrients (carbohydrate, protein, and fat), polyphenolic compounds, amino acids, fatty acids, minerals, fiber, carotenoids, and ascorbic acid. Prosopis flour has high protein and low carbohydrate and fat content, which can contribute to a healthy diet. Prosopis contain essential amino acids, such as isoleucine and valine, which do not exist in other grains. Prosopis can also be a good source of linoleic acid and minerals including potassium, magnesium, calcium, and phosphorus. Furthermore, Prosopis contains abundant phenolic compounds, such as flavonoids (especially Cglycosyl flavonoids), tannins, 4′-O-methyl-gallocatechin, mesquitol, and quercetin O-glycosides, but the content of hydrolysable tannins and anthocyanins is relatively low. Phenolic compounds are the most valuable content in Prosopis as they significantly contribute to the pharmacological value and nutraceutical potential in Prosopis. Prosopis has different pharmacological functions that can be divided into antioxidant activity, antihyperglycemic activity, analgesic and antipyretic activity, antitumor activity, respiratory and gastrointestinal activity, anticonvulsant activity, antibacterial activity, hypolipidemic and antiatherosclerotic efficacy, anti-inflammatory activity, adsorption potentialities, antihelminthic activity, apoptotic activity, antidepressant activity, and anticancer effects. Except for the high content of phenolic compounds, Prosopis can also be a good source of protein, galactomannans, and dietary fiber. e moisture content in Prosopis is relatively low, which helps to increase the shelf life of Prosopis products. However, the factors influencing the pharmacological activity and bioactivity in Prosopis are of concern, such as bioavailability of phytochemicals, antinutritional compounds, and effects of protein-phenolic compound interactions.
In the future, as a rich source of phenolic compounds, the structures and properties of phytochemicals in Prosopis necessitate further studies. In addition, it is vital to develop processing methods to maintain the nutritional compounds of Prosopis, when it is used as food. Furthermore, when Prosopis is grown, the use of pesticides should be strictly controlled, while the chemical compounds and nutritional properties affected by genetic diversity and different living conditions are worthy to be further researched. Finally, Prosopis has great potentiality to be applied in medicine, but it is still necessary to study the appropriate standards and reasonable dosage of different varieties of Prosopis.
Data Availability e data supporting this review are available from previously reported studies and datasets, which have been cited. e processed data are available from the corresponding author upon request.

Conflicts of Interest
e authors declare that they have no conflicts of interest.