In Vivo Anti-Inflammatory, Analgesic, Sedative, Muscle Relaxant Activities and Molecular Docking Analysis of Phytochemicals from Euphorbia pulcherrima

Euphorbia pulcherrima is an important medicinal plant that is used in a traditional system for its curative properties such as analgesic potency, antipyretic, anti-inflammatory, sedation potential, and antidepressant and cure of diseases such as skin diseases. This study deals with the isolation of two flavonoids namely spinacetin (1) and patuletin (2) from chloroform fraction of Euphorbia pulcherrima. The isolated compound spinacetin (1) and patuletin (2) were screened for in vivo anti-inflammatory, analgesic, sedative, and muscle relaxant effects. Compounds 1 and 2 were assessed against hot plate-induced noxious stimuli at various doses which showed excellent (p < 0.05) analgesic effect in a dose-dependent manner. The muscle relaxant activity was determined by traction and inclined screening model, both compounds showed significant muscle relaxant activity with time. The sedative potential of isolated compounds 1 and 2 was determined by the open field model, both compounds showed good sedation (p < 0.05) at 20 mg/kg. The anti-inflammatory potential of compound 1 was recorded by histamine-induced paw edema and carrageen paw edema model, and in both models, compounds 1 and 2 showed strong effect at 20 mg/kg. Binding orientations, binding energy values, and computed inhibition constants (Ki) values revealed that the studied compounds have a good to excellent inhibition potential against μ-opioid receptors and COX-2.


Introduction
Medicinal plants are a key source of various classes of secondary metabolites [1]. e medicinal importance of plants is correlated due to the presence of natural products.
ere are several bioactive natural products isolated and are major constituents of the modern medicinal system [1]. Many plants are investigated for new drug discovery because the drugs prepared from plants are more effective and have no toxicity. Many medicinal plants have several biomedical applications such as Alzheimer's diseases [2], neurodegenerative [3], depressive [3], and metabolic disorders [4] as well as promote health and prevent disease [5].
Euphorbia pulcherrima is a member of the family Euphorbiaceae. Euphorbiaceae is also known as spurge or Euphorbias family. Euphorbiaceae is the largest flowering family among the Anthophyta. is family comprises 5000 species and 300 genera throughout the globe. All members of this family are a key and rich source of bioactive natural products. Members of this family are distributed in Saudi Arabia, Pakistan, and India. Different species of this genus is famous for its folk usage for the cure of various ailments. ese species contain latex which is a rich source of bioactive secondary metabolites [6]. is genus comprises diterpenoids and other compounds which his documented for excellent anticancer and other properties [7]. For example, the essential oil isolated from E. hirta is used for the treatment of asthma and is also used as a mosquito repellent. Various phytochemicals including phytosterols, triterpenes, tannins, flavonoids, and polyphenols have been isolated from various parts of E. hirta [8]. Dry roots of E. kansui herbal remedy are used for the cure of ascites, edema, and cancer [8]. Various parts of dhudi (E. hirta) also comprise saponins, alkaloids, and flavonoids. E. hirta is employed for curing several ailments such as kidney stones, bronchial ailments, gastrointestinal problems, respiratory disease, and diabetes [9].
Euphorbia pulcherrima is also known as poinsettia or Christmas flower and is distributed on the Pacific coast of America as well as Mexico and Guatemala. E. pulcherrima is also found in various countries of Asia and Nepal [10]. e aerial part of Euphorbia pulcherrima is used in the traditional system for the treatment of ailments such as normal/ slow transit constipation, skin disease, and rise of milk secretion in nursing mothers. Also, E. pulcherrima documented for various in vitro and in vivo biological activities [11]. Some species of Euphorbiaceae has been documented for numerous pharmacological potential such as central nervous system, inflammation, fever, neuropharmacological, and hypnotic [12]. Flavonoids isolated from Euphorbia pulcherrima have reported moulting properties with Spodoptera littoralis [13]. Flavonoids documented from other plants have been reported for anti-inflammation, analgesic, phosphodiesterase, and antidiarrheal activities [14][15][16].
Based on extensive pharmacological applications on the family Euphorbiacea, there looks to be unsatisfactory information on E. pulcherrima. e current finding aimed at the isolation of flavonoids namely spinacetin (1) and patuletin (2) and their in vivo pharmacological potential. e voucher specimen number (PUP545)/UOP was retained at the herbarium of the mentioned department.

Extraction and Isolation.
e collected plant material was dried in shade, converted to powder, and then assessed to cold extraction with a polar solvent (methanol) for 20 days as per the reported method [17]. e extract obtained was filtered and then concentrated at reduced pressure and low through the rota evaporator. e crude extract 189 g was subjected to fractionation with various polarity solvents such as n-hexane, chloroform, ethyl acetate, and butanol. All fractions were subjected to think layer chromatography among which chloroform contain a maximum number of phytochemicals which was subjected for chromatographic analysis. Among chloroform fraction, 24.27 gm has exposed to column chromatographic analysis on silica gel and then eluted with a mixture of hexane and EtOAc (100 : 00 to 50 : 50). e column chromatography afforded 243 subfractions (AF1-TO AF243). e subfraction was combined based on TLC profile which yields ten subfactions (AFF1-AFF10). Among which AFF7 was subjected to repeated column chromatography on silica gel, eluting with hexane and EtOAc (40 : 60) which afforded compound 1 (1.62 g) and compound 2 (1.73 g). e chemical structure of isolated constituents 1 and 2 was identified by advanced spectroscopic analysis and comparing the spectral and physical data with reported data [18][19][20].

Animals.
In vivo biological screening of compounds was performed by using Balb/c mice of both sexes having weight (24-27 g

Anti-Inflammatory Screening.
e isolated flavonoids spinacetin (1) and patuletin (2) were assessed for anti-inflammatory screening with carrageen paw edema and histamine-induced edema procedure as per the published procedure [21]. e mice were divided into various groups each group comprise six animals. e distributed groups of animals consist of negative control (10 mL/saline) and positive control (diclofenac; loratadine; 5 mg/kg). Tested groups of divided animals were treated with isolated flavonoids spinacetin (1) and patuletin (2) (5, 10, 15, and 20 mg/kg). Exactly after 30 minutes of the intraperitoneal administration, 1% of carrageen (0.05 mL) and histamine (0.1 mL) were transdermally administered in the right paw of each mice. e inflammation of PW was observed for each tested mice for six hours after injection of carrageen and histamine with the help of a plethysmometer. e anti-inflammatory of both tested flavonoids (1 and 2) was calculated with the help of the following formula: where A is the paw edema of the control and B is the paw edema of tested flavonoids.

Analgesic
Activity. e isolated flavonoids spinacetin (1) and patuletin (2) were also subjected to analgesic screening according to our published procedure [21]. In this model, all animals were distributed to various groups, and each group consists of six mice. Among the entire groups, only one group of mice was administered with normal saline (10 mL/ kg) as a control for statistical calculation, and one group was administered with tramadol (5 mg/kg). e remaining groups of animals were treated with isolated flavonoids spinacetin (1) and patuletin (2) (5, 10, 15, and 20 mg/kg). e analgesic effect of tested compounds was identified through a hot-plate analgesiometer according to the reported method [21]. After 30 minutes of sample administration, every group of mice was screened for analgesic potential through hot plate and the exact time of the latency was observed in seconds. e percentage analgesic effect was identified as per the standard method [22].

Sedative Activity.
e isolated flavonoids spinacetin (1) and patuletin (2) were also screened for sedation properties [23]. All animals were divided into various groups and each group consists of 6 animals. Among the entire group, the control was administered with 0.5 mg/kg of diazepam. e sedative potential of flavonoids spinacetin (1) and patuletin (2) was performed as per the reported method. According to this procedure, after 30 minutes of administration, every group of animals should be placed in a special design box containing lines, the line crossed by every mouse observed. Amount tested groups of animals, that group of animals with the delayed movement was considered as a sedative while the rest of groups of mice which crossed more lines were considered none sedative.

Muscle Relaxant Screening
e isolated flavonoids spinacetin (1) and patuletin (2) were also screening for muscle relaxant potential in an include plane procedure as per the published method [23]. Include plane model composed 2 plywood boards linked to each other among which one side of the plywood board shaped the base and the other side of the plywood board was connected to the base at almost 65 degrees. All animals were divided into various groups, and each group consists of 6 animals. Animals of a different group in this screening were treated with standard (diazepam; 1 mg/kg), normal saline (10 mL/kg), and isolated flavonoids spinacetin (1) and patuletin (2) (5, 10, 15, and 20 mg/kg). After treatment of all samples including spinacetin (1) and patuletin (2), standard, and saline with various time intervals (30, 60, and 90 minutes), the animals was observed to fall or not for around 30 seconds on putting on the upper part of an inclined plane.

Traction Method.
e isolated flavonoids spinacetin (1) and patuletin (2) were also screened for muscle relaxant potential by using the traction model [23].
is model comprises a metal wire cover with rubber, where ends of this metal wire were tightly stretched and reinforced with stands and were kept about 60 to 70 cm above the bench. To screen all samples, the animals were distributed into fourteen groups and each group comprises 5 animals. Among the groups, one group received distilled water, one received diazepam which was considered as a negative and positive group. e remaining groups of animals were administered with spinacetin (1) and patuletin (2) in various doses such as 5, 10, 15, and 20 mg/kg i.p. e muscle relaxation properties were observed for all groups of animals at various time intervals, i.e., 30, 60, and 90 minutes. Every mouse in this assay was suspended on the metal wire from hind legs, and the floppy time was recorded for nearly 5 seconds. is failure of mice to hang in the metal wire in less than 5 sec indicated the presence of muscle relaxant property for spinacetin (1) and patuletin (2).

Toxicological Profile.
e purified spinacetin (1) and patuletin (2) was screened for toxicological study as per reported method [23]. All animals were distributed into respective groups, each group comprising six animals. e purified spinacetin (1) and patuletin (2) were administered to various groups of animals at doses 10, 20, 100, and 200 mg/kg. e tested animals were kept under observation for 2 days. e mortality was calculated from the number of dead and surviving animals of each group.

Statistical Analysis.
e results of anti-inflammatory, analgesic, sedative, and muscle relaxant screening are represented as the mean ± standard error of the mean (SEM) to identify the significant difference in all tested animals. e results achieved were evaluated to one-way analysis of variance (ANOVA). e analysis was done with help of Dunnett's multiple assessment screening using GraphPad Prism 5.

Molecular Docking
e isolated spinacetin (1) and patuletin (2) were docked in the binding site of COX-2 and opioid receptors by using Molecular Operating Environment (MOE) software (201.2016.0802) and AutoDock (v 4.0) [22,24]. Online database of biological macromolecules RCSB PDB was used to download three-dimensional (3D) crystal structures of selected proteins. e accession codes of these proteins are 1CX2 for COX-2, 4COF for μ-opioid receptor, and 5C1M for GABA A receptor.
Next, we performed validation of docking protocol by using the redock method. All the native ligands were extracted and docked into the binding site of their respective proteins. A comparison between redocked conformation and experimental confirmation showed a root mean square deviation with a limit (<2.0Å).
2D structures of isolated compounds were drawn in ChemDraw and then exported to the MOE and AutoDock. e structures were saved in a 3D format in MOL2 (for AutoDock) and molecular database (.mdb) format for MOE, and saved as 3D structures. Preparation of downloaded proteins was performed by using our previously reported methods [25]. For each compound, we set the default parameters for docking simulations. Finally, 3D and 2D Evidence-Based Complementary and Alternative Medicine analysis of each docked pose was performed by using MOE and a discovery studio visualizer [25]. (1) and Patuletin (2). Spinacetin (1) and patuletin (2) were purified from chloroform soluble fraction of E. pulcherrima through chromatographic analysis. e structure of spinacetin (1) and patuletin (2) (Figure 1) was characterized by advanced nuclear magnetic resonance spectroscopy and mass spectrometry.

Anti-Inflammatory Effect.
e results of spinacetin (1) and patuletin (2) showed that it exhibited 35.22 and 46.00% attenuation in carrageenan-induced paw edema model which reached a maximum of 79.22 and 89.01% after 3 rd hours at 20 mg/kg and remain good in the 5 th hours. Both tested compounds showed mild effect at 5, 10, and 15 mg/kg as compared to 20 mg/kg. Diclofenac sodium was used as a positive control in this investigation which showed outstanding effect as compared to spinacetin (1) and patuletin (2) (Figure 2).

Muscle Relaxant Effect. Spinacetin (1) and patuletin (2)
isolated from E. pulcherrima were also screened for muscle relaxant effect on inclined plan and traction model, which showed promising muscle relaxant potential (Table 3). Spinacetin (1) and patuletin (2) exhibited significant effects in the dose-and time-dependent manner. Both tested compounds possess good activity in both models. e effect of both compounds was excellent from the start of the experiment against the standard drug.

Toxicological Study.
e purified spinacetin (1) and patuletin (2) was screened for toxicological study in the higher doses 10, 20, 100, and 200 mg/kg. Spinacetin (1) and patuletin (2) was administered to the animals intraperitoneally (i.p). e tested animals were kept for 2 days under observation. e number of surviving and dead animals was recorded. All groups of treated animals survived all tested doses. Interestingly, spinacetin (1) and patuletin (2) was found safe at all tested doses.

Docking Studies on μ-Opioid and GABA A Receptors.
We have performed the docking simulations on GABAA receptors as well as on μ-opioid receptors. e accession codes for the downloaded proteins from the protein data bank are 5C1M for μ-opioid and 4COF for GABA receptors. After the validation of the docking protocols, we docked spinacetin (1) and patuletin (2) into the binding sites of the downloaded and prepared targets.
We docked isolated compounds into the binding site of COX-2 isoform (PDB Id: 1CX2). 3D/2D interaction plots of the studied compounds are shown in Figure 6. It can be observed from these interaction plots that both compounds interact with the amino acid residues (His90, Gln192, Ser353, and Arg531) present in the selectivity pocket of the COX-2. e hydroxyl groups of compounds 1 and 2 form hydrogen bond interactions with selectivity pocket residues His90, Ser353, and Arg513. Ile517 also establishes a hydrogen bond with the hydroxyl group of compound 1 (Figures 6(a) and 6(b)).
Furthermore, we performed the docking studies by using the

Discussion
E. pulcherrima is employed for multimedicinal usage for the central analgesic potency, antipyretic, anti-inflammatory, sedation potential, and antidepressant and for the cure of skin diseases. Due to the diverse biological potential, the 4 Evidence-Based Complementary and Alternative Medicine isolation and structure determination of secondary metabolites followed by in vivo biological screening is important to identify the secondary metabolites responsible for the diverse biological application. In this finding, compounds 1 and 2 were isolated and screened for analgesia, muscle relaxation, sedative, and anti-inflammatory properties. Prostaglandin is an important product of arachidonic acid by cyclooxygenase enzyme. Majority of the cyclooxygenase enzyme inhibitors are recognized as active painkillers, also as anti-inflammatory agents [26,27]. It is a big challenge for a researcher to discover new potent and less or nontoxic inhibitors. Spinacetin (1) and patuletin (2) also attenuated inflammation in both tested models, i.e., carrageenan and histamine. Carrageenan paws edema model consists of two phases: the initial phase edema is recognized to the local release of histamine serotonin and bradykinins while the further phase is due to overproduction of PG [27]. In the tested model, spinacetin (1) and patuletin (2) exhibited significant analgesic activity against the standard drug.
e muscle relaxant effect of spinacetin (1) and patuletin (2) in both models that is inclined plan and traction model was analysed as per well-established methods. Spinacetin (1) and patuletin (2) exhibited a strong muscle relaxant effect. In both tested models, the relaxation of muscle was assessed after 30, 60, and 90 minutes of treatment of spinacetin (1) and patuletin (2). An outstanding muscle relaxant effect was observed after 60 minutes of spinacetin (1) and patuletin (2) administration at a higher dose.
Similarly, spinacetin (1) and patuletin (2) were also evaluated for their sedative properties which showed excellent activity. e sedative activity of these secondary metabolites has been recognized with their antihistaminic potential [28]. e antihistaminic potential may be similar to H1 receptor blocker, i.e., pheniramine which has an excellent sedative potential; thus, our screen compounds (1 and 2) have outstanding sedative potential. From our outstanding results, we recommend researchers for comprehensive and detailed screening as leading molecules for curing various diseases. e excellent anti-inflammatory, analgesic, sedative, and muscle relaxant potential of spinacetin (1) and patuletin (2) provide a strong scientific background for folkloric use of E. pulcherrima for treatment of various diseases.
To further support the in vitro result findings, we performed molecular docking analysis on the selected target receptors. First of all, docking simulations were performed on GABAnergic and μ-opioid targets. Both isolated compounds showed interactions with the amino acid residues of the studied targets. μ-Opioid receptor (μOR) and membrane proteins are considered as key therapeutic targets for pain. e binding of ligands to the target identifies the responses which are resulted into the activation of signaling pathway. A number of crystal structures have been solved for μOR that enable the drug discovery scientists to design a new ligand. Insights into the binding pattern of native agonist BU72 (PDB 5C1M) revealed that it interacts with His54, Asp147, and Tyr148 via hydrogen bond interactions. Here, in our study, the phenyl ring of spinacetin (1)    In the next step, we studied the inflammation pathway via COX-2 inhibition. Carrageenan-induced paw edema model is considered as COX-2-dependent model of inflammation. Spinacetin (1) exhibited excellent Ki value in submicromolar concentration (Ki � 0.631 μM). Binding pose revealed that the isolated compound is more oriented towards the additional or the selectivity pocket of COX-2 and established significant hydrogen bond interactions with the key amino acid residues (His90, Gln192, and Arg513).

Conclusions
It is concluded that spinacetin (1) and patuletin (2) isolated from E. pulcherrima showed excellent anti-inflammatory, analgesic, sedative, and muscle relaxant effects. us, our current research provides a scientific rationale to the traditional use of E. pulcherrima for the cure of various diseases. Furthermore, spinacetin (1) and patuletin (2) are outdating candidates for detailed research study to find its clinical application. Compounds 1 and 2 showed excellent (p < 0.05) analgesic effect in a dose-dependent manner. e muscle relaxant activity results showed significant muscle relaxant activity with time, while sedative potential of compounds under study showed good sedation (p < 0.05) at 20 mg/kg. Moreover, the anti-inflammatory potential of compounds determined via histamine-induced paw edema and carrageen paw edema model showed strong effect at 20 mg/kg. Inhibition constants (Ki) values computed via the AutoDock software revealed that these compounds have a good to excellent inhibition potential against GABA and COX-2, while binding orientation and interaction plots also showed binding affinities the studied targets. e excellent antiinflammatory, analgesic, sedative, and muscle relaxant potential of spinacetin (1) and patuletin provides a strong scientific background for folkloric use of E. pulcherrima for treatment of various diseases.
Data Availability e spectroscopic data of isolated compounds (1-3) are available on request to the contact authors. e graphical abstract is given in supplementary data.

Conflicts of Interest
ere are no potential conflicts of interest associated to this project.