Response Surface Methodology Applied to the Optimization of the Preparation of Antioxidant and Antidiabetic Extracts from Phragmanthera capitata (Spreng.) Balle: Effect of Particle Size, Powder-to-Solvent Ratio, and Temperature

Phragmanthera capitata is a medicinal plant used in traditional medicine to treat several diseases, including diabetes. Its antioxidant properties and inhibitory effects on enzyme-carbohydrate digestion activities have been demonstrated. The present study aimed to provide data that can contribute to rationalizing the preparation of antioxidant and antidiabetic extracts from this plant. P. capitata (whole plant) growing on Persea americana was harvested at the fruiting stage. A response surface design-type central composite was applied to maximize the extraction yield, phenolic contents, and antioxidant and antidiabetic properties of the ethyl acetate extract of P. capitata. The influencing extraction factors were temperature, powder particle size, and solvent-to-powder ratio. The total phenolic content, total antioxidant capacity (TAC), DPPH scavenging ability, ferric reducing antioxidant power (FRAP), and antidiabetic (α-amylase and α-glucosidase inhibitory) effects of the extracts were determined using conventional methods. A temperature above 55°C contributed to the degradation of the extract, which was reflected in the GC-MS profile by a significant reduction in the number of compounds it contained. The optimal conditions were defined as 24.42°C for temperature, 250 µm powder particle size, and 8.30 (v:w) solvent-to-powder ratio. This extraction protocol resulted in more than twice the extraction yield (3.05%), TTC (62.30 mg TAE/g), TAC (41.41 mg AAE/g), FRAP (186.56 mg AAE/g), and α-amylase (IC50 15.05 µg/mL) and α-glucosidase (IC50 21.14 µg/mL) inhibitory activities compared to our previous results. Additionally, these optimal conditions led experimentally to the extraction of higher phenolic content and to the attainment of higher antioxidant and antidiabetic activity, which closely matched the predicted values. Using these conditions, it is possible to prepare an antidiabetic phytomedicine from P. capitatathat can prevent oxidative stress complications. However, further complementary studies should be carried out considering other factors that influence the composition and pharmacological properties of the extract.


Introduction
Traditional medicine, particularly herbal medicine, plays an important role in primary healthcare systems in developing countries [1]. is is because of it easy access, it is generally well accepted by populations, has little or no toxic effects and can contribute to the economic growth of countries [2]. In some cases, it is the only alternative for rural populations. However, its practice remains mainly empirical and consequently presents significant limits [3]. Some of these limits include variation in chemical composition and pharmacological effects with ecological factors, plant intrinsic factors, and factors linked to the preparation of the phytomedicine itself. Indeed, herbal medicines are known to be complex mixtures comprising many constituents with qualitative and quantitative compositions depending on the batch of raw material and the methods of extract preparation (nature of the solvent, proportion, duration and temperature of extraction, etc.); it is almost impossible to obtain extracts of completely similar composition and therapeutic efficiency at any time [4]. In the light this, the success of phytomedicine usage requires, above all, a good level of technical and scientific mastery of standardization. Standardization is needed to ensure consistent pharmaceutical quality for herbal medicines by permitting standardized quality procedures at all stages of manufacturing, from raw materials to phytomedicine production [5].
Extraction is one of the main steps in the preparation of phytomedicines or in the discovery of potentially bioactive compounds from raw plant material. is process is influenced by many factors, such as the nature of the solvent, extraction temperature, time, powder-to-solvent ratio, size of the vegetable powder particles, and pH [6]. ese factors influence not only the extraction yields but also the concentrations of the active ingredients and therefore the biological properties of the extract [7]. Optimization of the extraction is important, and the response surface methodology seems to be one of the best indicated. It allows us to explore the relationships between the dependent and independent parameters involved in an extraction process [6]. e first step consists of choosing the appropriate medicinal plant candidate.
Phragmanthera capitata, commonly called African mistletoe, is a woody semiparasitic shrub, belonging to the Loranthaceae family. It can grow on various hosts in secondary jungles, plantations, and bush savannah areas, from Sierra Leone to Western Cameroon, Fernando Po, extending across the Congo basin to RD Congo, Nigeria, Gabon, Ivory Coast, and Angola [8,9]. Leaves, stems, or whole plants are widely used in traditional medicine to treat approximately 38 arrays of ailments [10,11]. e most commonly used modes of preparation by healers are decoction, maceration, and infusion in water and palm wine [10,12]. Some pharmacological studies carried out on various crude extracts and purified fractions have confirmed most biological properties of this plant against a number of ailments ranging from diabetes [12] to hypertension, epilepsy and sedation [13], infertility [14], obesity [15], bacterial and fungal infections [16], cancer, and inflammation [17]. Moreover, Ohikhena et al. [18] and Feudjio et al. [19] showed the antidiabetic activity of P. capitata through inhibition of α -amylase and α-glucosidase catalytic activity by ethyl acetate extract. Furthermore, the medicinal value of this plant is great due to its diversified phytochemical component content, including phenolics, flavonoids, alkaloids, and tannins [20] and its nontoxicity in experimental animals at appropriate doses [9].
is plant is therefore a suitable candidate for the formulation of antioxidant phytomedicine against oxidative stress diseases such as diabetes.
In a previous study, our team showed that the chemical composition and the antioxidant and antidiabetic properties of the extracts of this plant were influenced by the host plant, phenological stage, and solvent used to prepare the extracts.
is plant, harvested from Persea americana and Psidium guajava during fruiting, gave better pharmacological results when extracted with ethyl acetate. us, the present study is a continuation of that work with the objective of optimizing the preparation of extracts with high antioxidant and antidiabetic effects using surface response methodology.

Plant Material. Phragmanthera capitata growing on
Persea americana was harvested at the fruiting stage at 9 am in April 2018 in Bamendou village, Menoua division in the West Region of Cameroon. Authentication of the plant and its host was performed at the Cameroon National Herbarium (HNC) in Yaoundé by comparison of our plant specimens with registered voucher specimens nos. 24673/ SRF/CAM (SRF: Cameroon forest reserve society) for P. capitata and 57756/HNC for P. americana. e plant sample was cleaned and dried for 3 weeks in a ventilated room at ambient temperature (22 ± 2°C). e dried plant material was ground using an herbal grinding system successively fitted with mesh sieves 14, 25, and 60 to obtain powder particle sizes of 250, 707, and 1410 μm.

Study Design.
A response surface design-type central composite (RSD-CC) was used to investigate and optimize the extraction yield, total phenolic content, total flavonoid and flavonol content, total tannin content, total antioxidant capacity, DPPH scavenging activity, ferric reducing antioxidant power (FRAP), and α-amylase and α-glucosidase enzymatic inhibitory activity. e controlled factors, at three different levels, selected based on the data reported in the literature and preliminary assays, included temperature (A, 22 ± 2°C/48 h, 55°C/15 min, and 70°C/15 min), powder particle size (B, 250 µm, 700 µm, and 1400 µm), and solventto-powder ratio (C, 3 : 1, 5 : 1, and 10 : 1 (v:w)) ( Table 1). e measured effects (responses) included extraction yield, TPC, TFC, TFnC, TTC, FRAP activity, DPPH scavenging activity, and α-amylase and α-glucosidase extract inhibitory effects. From the performed experimental design, 27 experiments were conducted (Table 2). e relationship between the responses and the factors was described and fitted into the following polynomial regression equation: (1) In this equation, Y is the predicted response; a 0 is the interception coefficient; b 1 , b 2 , and b 3 are the linear terms; c 12 , c 13 , and c 23 are the interaction terms; and d 1 , d 2 , and d 3 represent the squared coefficients of the independent variables. e model was statistically significant when the coefficient of determination was close to 100% and its probability of nullity α was less than 5% [21].

Preparation of Extracts.
e corresponding powder particle size of the plant material (250, 700, or 1400 µm) was mixed with the specified solvent volume-to-powder proportion (1 : 3, 1 : 5, or 1 : 10) for the extraction process. e suspension was kept in a process of dynamic steeping for a specified time and temperature according to the matrix design of Table 2. A stirring heater set at 600 rpm was used to keep the suspension in the conical flask equipped with a reflux system under the specified temperature. Each resulting mixture was vacuum-filtered using Whatman filter paper no. 1, and the filtrates were evaporated at 40°C under vacuum through a Buchi R-210 evaporator. e resulting extracts were then dried at 40°C in an oven for 24 hours to remove the residual solvent and stored at 4°C for further studies. e extraction yield was calculated based on the initial dried powder mass [22]. Each extraction assay was performed in triplicate.

GC-MS Analysis of Extracts.
GC-MS was performed for ethyl acetate extracts achieved under defined optimal extraction conditions. is analysis was performed on a Perkin Elmer Clarus 600 GC/MS N6520204-E apparatus.
e Perkin Elmer Clarus 600 GC System was fitted with an Elite-5MS capillary column (30 m long, 0.25 mm inner diameter, 0.25 µm film thickness, and highest temperature of 350°C) coupled to a Perkin Elmer Clarus 600C MS. Helium (99.99% purity) was used as the carrier gas at a constant flow of 1 mL/min. e oven temperature was programmed from 40°C (held for 2 min) to 280°C (held for 10 min) at a rate of 5°C/min. e crude extracts were solubilized in chloroform and filtered with a syringe filter (Corning, 0.45 µm). A volume of 1 µL of the filtered extracts was injected into the column at a split ratio of 1 : 20. e percentage composition of each extract was expressed as a percentage of the peak area. e mass spectra were acquired within 50-550 m/z. e chemical constituents of the extracts were identified using gas chromatography retention indexes and mass spectra matching those of the standards available in the National Institute of Standards and Technology (NIST) library 2014.

Determination of Phenolic Content, Antioxidants, and Inhibitory Activities of α-Amylase and α-Glucosidase of the Extracts.
e total phenol content (TPC), total flavonoid Evidence-Based Complementary and Alternative Medicine 3 content (TFC), total flavonol content (TFnC), total tannin content (TFC), total antioxidant capacity (TAC), ferric reducing antioxidant power (FRAP), and 2,2-diphenyl-1picrylhydrazyl radical scavenging ability (DPPH-RSA) of the extracts as well as the inhibition of α-amylase and α-glucosidase by these extracts were determined as previously described [19]. Each assay was carried out in triplicate.

Statistical
Analysis. Minitab 19.0 software was used to generate the test matrix, to optimize the extraction conditions of P. capitata, and to analyze the results of the response surface design-type central composite. e response surface and iso-response curves used to visualize the combined effects of two independent variables on responses were generated with SigmaPlot 12.0 software. e adequacy of the model was determined by evaluating the lack of fit, the coefficient of determination (R 2 ), the adjusted coefficient of determination (R 2 adjusted), and the regression (p test values) obtained from the ANOVA. e optimal extraction conditions maximizing the phenolic content, antioxidant and antiglycation activity, and α-amylase and α-glucosidase inhibitory effects were selected using the desirability function approach in Minitab 19.0 software.

Effects of Combined Extraction Parameters on Responses.
e results of the responses for the 27 runs following the experimental design are shown in Tables 3 and 4. All combined independent variables (extraction temperature, powder particle size, and solvent-to-powder ratio) had a variable effect on the 10 responses (extraction yield, TPC, TFC, TFnC, TTC, DPPH-RSA, and α-amylase and α-glucosidase inhibitory activities). Among these, the highest extraction yield (3.08%), TPC (727.95 GAE/g), antioxidant activities (TAC of 42.188 mg AAE/g and FRAP value of 187.76 mg AAE/g), and α-amylase and α-glucosidase inhibitory activities (IC 50 of 13.646 and 21.119 µg/mL, respectively) were observed in experimental run 16 under extraction conditions of 22°C/30 min extraction temperature, 250 µm powder particle size, and 5 : 1 mL/g solvent-topowder ratio.

Optimum Conditions of Extract Preparation for Phenolic
Content and Antidiabetic Properties. Pareto charts were used to identify factors (temperature (A), powder particle size (B), and powder-to-solvent ratio (C)) that significantly affected phenol content, antioxidant activity, and inhibitory activity  (Figures 1 and 2) show that the extraction conditions influenced the responses at different levels, but there were no valid interactions between them. e extraction yield, FRAP, and α-amylase inhibitory activity of the extracts were mostly influenced by powder particle size and extraction temperature (Figures 1(a), 2(b), and 2(d)). In contrast, the powder particle size and powder-to-solvent ratio had a significant effect on TPC (Figure 1(b)), while extraction temperature and solvent-to-powder ratio mostly influenced TFC, TFnC, TTC, and α-glucosidase inhibition (Figures 1(c)-1(e)). e extraction temperature significantly affected TAC and DPPH-RSA (Figures 2(a) and 2(c)). Furthermore, the second-order effect of extraction temperature (AA) produced a significant effect on the TFC, TFnC, and liberation of α-glucosidase inhibitors (Figures 1(c)-1(e)), while the quadratic effect of the powder-to-solvent ratio (CC) was found to produce a significant effect on the TFC, TTC, and liberation of ferric ion reducing antioxidants and α-amylase inhibitors (Figures 1(e), 2(b), and 2(d)).
From the multiple linear regression analysis of the 27 data entries, the relationships between the response and factors studied were second-order polynomial functions (Table 5). From these regression models, the extraction yield, TFC, TFnC, TTC, TAC, FRAP, α-amylase, and α-glucosidase effects were highly significant (p value = 0.000), with coefficients of determination (R 2 ) ranging from 72.92 to 95.31. However, the regression models for data on the TPC and DPPH-RSA did not fit well, giving coefficients of determination R 2 of 38.13 and 61.76, respectively.
Surface response plots illustrate the evolution of each response depending on the level of the most influencing factors ( Figure 3). It was noted that the extraction yields increased when the powder particle size and extraction temperature decreased. Likewise, TAC, FRAP, DPPH-RSA, and α-amylase inhibition (low EC 50 ) increased with decreasing extraction temperature and powder particle size (Figures 3(f )-3(i)). TFC, TFnC, TTC, and α-glucosidase catalytic inhibition (low EC 50 ) increased when the solventto-powder ratio increased at low extraction temperatures (Figures 3(c)-3(e) and 3(j)).
Iso-response curves showed the optimal areas (hatched parts) corresponding to the maximal response ( Figure 4). us, the extraction yields, TAC, FRAP, DPPH-RSA, and α-amylase inhibition (low EC 50 ) were highest in the areas limited by extraction temperatures below 30°C and powder Evidence-Based Complementary and Alternative Medicine particle sizes less than 400 µm (Figures 4(a) and 4(f )-4(i)). However, TFC, TFnC, TTC, and α-glucosidase catalytic inhibition (low EC 50 ) were highest in the areas limited by a powder-to-solvent ratio of 8 : 1 and extraction temperatures below 30°C (Figures 4(c)-4(e) and 4(j)). e optimal extraction conditions for the yield, phenolic content, and antioxidant and antidiabetic activities of P. capitata using ethyl acetate as the extraction solvent were determined by superimposing their 3D response surfaces. e predicted optimum extraction conditions for maximum response (desirability close to 1) were obtained for 24.42°C temperature, 250 µm powder particle size, and a 10 : 83 (g/mL) powder-to-solvent ratio ( Figure 5). e experimental responses obtained after running the assay under these optimal extraction conditions were compared to the predicted response values from the central composite experimental factorial design runs (Table 6). e experimental values obtained from the confirmation runs were within the predicted confidence interval values, thus proving the accuracy of the model.  Figure 1: Pareto diagram highlighting the combined effects of temperature, powder particle size, and powder-to-solvent ratio on phenol content of ethyl acetate extract of Phragmanthera capitata growing at fruiting on Persea americana. AA: second-order effect of extraction temperature significantly affecting the extraction yield, TFC, and TFnC; BB: second-order effect of powder particle size significantly affecting the TTC; CC: second-order effect of solvent-to-powder ratio significantly affecting the extraction yield, TFC, and TTC. 6 Evidence-Based Complementary and Alternative Medicine acetate extract (prepared using optimal extraction conditions: extraction temperature of 24°C, powder particle size of 250 µm, and a powder-to-solvent ratio of 10 : 83 g/mL). e retention unit (ui) was used to characterize each compound instead of the retention time, which is variable as the analysis conditions. e major compounds were benzene, 1-(1,5dimethyl-4-hexenyl)-4-methyl-(53.14%); 2-butanone, 4-(4hydroxy-3-methoxyphenyl)-(29.91%); tetradecane (7.35%); hexadecane (3.21%); and octanal (2.10%). Seven compounds were identified in the extract by applying an extraction    Evidence-Based Complementary and Alternative Medicine temperature of 55°C with an increase in the proportions of n-hexadecanoic acid (18.42%) and oxirane propyl (0.91%). Oxirane propyl (98.72%) was the only compound in the extract prepared at 70°C ( Figure 6 and Table 8).

Discussion
Phenolics or polyphenols, including flavonoids, have received greater interest, as they have shown high antioxidant power and are correlated with many health benefits, including diabetes, cancer, and cardiovascular diseases [23]. Due to the chemical diversity and the many parameters that can influence the efficiency of antioxidant phenolic extractions from the plant, each phenolic source demands an individual approach for extraction and optimization [24]. Response surface design-type central composite (RSD-CC) is useful for the optimization of the extraction process and for studying the relationship between extraction parameters, phenolics, and correlated biological activities. In this study, the extraction temperature, powder particle size, and solvent-to-powder ratio were examined for their impact on the extraction yield, phenolic content, antioxidants, and carbohydrate digestive enzymes (measured effects or responses) of P. capitata extracts. From the RSD-CC used, all assessed extraction parameters showed a significant effect on all the responses without valid interaction between them. e analysis of variance (ANOVA) for the measured effects (except total phenol content and DPPH-RSA) showed that the calculated and adjusted coefficients of determination (R 2 ) were close to unity, ranging from 72.92% to 99.58%.
is outcome indicates that the mathematical models of these plans were significant and that more than 72% or 99% of the response variables (extraction yield, TFC, TFnC, TTC, TAC, FRAP, and enzyme inhibitory activities) could be described by the RSD-CC model. In fact, according to Radojkovi et al. [25] and Azahar et al. [26], the closer the calculated and adjusted R 2 values are to unity, the better and more significant the empirical model fits the actual data, and  A: temperature (°C), B: powder particle size (µm), and C: solvent-to-powder ratio (v:w).

Evidence-Based Complementary and Alternative Medicine 11
used to optimize the conditions under which P. capitata extracts should be prepared to obtain higher extraction yields, phenolic content, antioxidants, and antidiabetic activities.
Temperature was the main parameter that influenced the liberation of phenolics as well as antioxidants (through radical and redox reactions) and α-amylase and α-glucosidase inhibitors from P. capitata.
ere was a significant quadratic effect (p < 0.05) of temperature on TFC, TFnC, and α-glucosidase inhibitory activity, indicating both positive and negative effects on these phenolics and related activities. Increasing the extraction temperature from 22 to 40°C gave extracts with the highest phenolic content, while between 40 and 70°C, the amounts of phenolics, antioxidants, and inhibitors of enzymatic activities were significantly decreased. It is well known that a high extraction temperature softens the plant tissue and weakens phenol-polysaccharide and phenol-protein interactions, thus promoting the migration of molecules into the solvent and enhancing the extract efficiency related to them [27,28]. is result was in accordance with a previous study. Roselló-Soto et al. [29] reported that the TPC and antioxidant activities of tiger nuts ethanol extracts increased from 25 to 37°C and then decreased. Altemimi et al. [30] concluded that the amounts of rutin and quercetin from peach and pumpkin extracts quickly increased and reached the maximum value with increasing extraction temperature from 30 to 41.08°C and then decreased due to thermal degradation of these flavonoids. Indeed, it was found that higher temperatures promoted degradation reactions such as the epimerization and oxidation of compounds, which could explain the loss    extract at 55°C; and 1 compound in the extract achieved at 70°C. Rutin and quercetin were also previously identified in the HPLC profile of P. capitata ethyl acetate extract at 22°C [19]. ese compounds could be linked to the broad spectrum of activity of P. capitata. Indeed, 2-butanone, 4-(4hydroxy-3-methoxyphenyl)-is a polyphenolic alkanone, known antioxidant [32], and potential α-amylase and α-glucosidase inhibitor [33]. Rutin and quercetin are reference antioxidants [34] due to their redox properties, allowing them to scavenge free radicals, donate hydrogen atoms or electrons or chelate metal cations [35], and act as α-glucosidase and α-amylase inhibitors [36]. Butan-2-one, 4-(3-hydroxy-2-methoxyphenyl)-could be the product of the degradation reaction in the extract at 55°C, which, associated with the thermal degradation of rutin and quercetin, could contribute to the decrease in antioxidants and enzymatic inhibitory activities of the resulting extract. is result was also in accordance with a previous study that stated a decline in phenolic content and antioxidants from 55 to 65°C in Funtumia elastica [27]. After temperature, the particle size is another important parameter controlling the kinetics of mass transfer and the access of the solvent to soluble compounds [37]. In this study, increasing the extraction temperature from 22 to 30°C combined with the reduction in powder particle size below 400 µm promoted the migration of antioxidants and α-amylase inhibitors into the extraction solution. is can be explained by the fact that the reduction in plant powder particle size leads to an increase in solubility and diffusion of bioactive components in the plant matrix due to the increase in the surface area available for mass transfer between the particle and the solvent [38,39]. Makanjuola et al. [40] reported that the phenolic content and antioxidant properties in ground tea with particle sizes of 0.425 mm were higher than those in ground tea with particle sizes of 1.180 mm. A rapid increase in polysaccharide yield was observed when the particle size of Codonopsis pilosula powder decreased from 420 to 180 μm, and the optimal particle size was 180 μm; the maximum polysaccharide yield was 15.56% [41]. In contrast, catechin content and antioxidant properties in hydroalcoholic extracts of green tea decreased for particles of sizes less than 50 μm, showing that the smallest particles can sediment and decrease the contact between particles and the extraction solvent, thereby reducing mass transfer efficiency [42]. us, the critical particle size should be defined for each plant matrix, hence the importance of optimization.
ere was also a significant positive quadratic effect of the solvent-to-powder ratio on TFC, FRAP, and α-amylase inhibitory activity (P < 0.05), showing a positive effect on  TFC. With extraction temperatures ranging from 22 to 30°C, the higher this ratio was, the higher the extracted TFC, antioxidant, and α-amylase inhibitors were. An increase in solvent volume was linked to more extraction efficiency, which could be due to the faster diffusion rate and better mass transfer leaching out of phenolic components from plant raw materials due to increases in the concentration gradient as a driving force [43][44][45]. e solvent-to-powder ratio beyond 5 : 1 mL/g was the most effective, in agreement with Predescu et al. [46], whose study reported that a solidto-solvent ratio of 10 : 1 mL/g was the most effective for phenolic compound and antioxidant extraction from some plants. However, Andres et al. [47] reported a decrease in TPC in craft brewers' spent grain with increasing liquidsolid ratios, which took place until a 16 : 1 ratio, and a plateau was reached beyond that ratio. Montenegro-Landívar et al. [48] did not observe a significant effect on TPC when the solid/liquid ratio increased from 10 : 1 to 100 : 1 (v/w) when applying water extraction in orange and spinach wastes. us, Montenegro-Landívar et al. [48] concluded specifically that, apart from improving extraction yields, the knowledge of the optimal amount of solvent to use is of economic relevance.
Under the optimal conditions, the predicted values of all the responses were close to the experimental values, and all the experimental data were within ±95% prediction intervals, which confirms the accuracy and validation of the model. erefore, it can be concluded that the model from RSD-CC was accurate and reliable enough for predicting the phenolic content, antioxidants (through radical and redox reactions), and inhibitors of α-amylase and α-glucosidase activities. Previously, the extraction parameters of bioactive compounds have been successfully optimized using the RSD-CC method, and some of them are in agreement with our optimized protocol to a lesser extent. Clodoveo et al. [49] found that the maximum polyphenol extraction efficiency from Ceratonia siliqua was achieved under the optimized factor combination of 35°C/15 min, 5 : 1 mL/g water-topowder ratio, and 0.3 mm powder particle size. e standardized conditions for the extraction of polyphenols from pomegranate peel were found to be extraction room temperature of 28 to 30°C, solid-to-water ratio of 1 : 10, and particle size in the range of 100 to 400 μm [50]. In contrast, the optimized conditions for nutraceuticals and antioxidant extraction from mulberry leaves were 70°C extraction temperature, extraction time of 40 min, water-to-leafpowder ratio of 40 : 1 mL/g, and particle size of 25 µm [43]. Abu Bakar et al. [6] determined a temperature of 79.07°C for 17.42 min with a solid-to-liquid ratio of 1 : 20 g/mL as the best extraction conditions for TPC, TFC, and xanthine oxidase inhibitory activity of Euphorbia hirta. Furthermore, an extraction temperature of 72.06°C and an ethanol-tosolvent ratio of 1 : 22 g/mL were the optimal conditions for the highest extraction yield and PTP-1B and α-glycosidase enzyme inhibition rates from Kursi Wufarikun Ziyabit [22]. ese different results can be ascribed to the raw material studied, extraction methods and analyses, and the chemical nature of phenolic compounds in plant tissues.

Conclusion
e present study focused on certain extraction parameters to maximize the yield and biological properties of extracts obtained from Phragmanthera capitata whole plants. From the obtained results, this was achieved by applying the following extraction parameters: a temperature of 24.42°C, powder particle size of 250 µm, and solvent-to-powder ratio of 8.30 mL/g. ese conditions led to a higher yield and higher phenolic content of the extract and to better antioxidant and antidiabetic activities. Furthermore, the identified compounds in the ethyl acetate extract could be used as standardization markers of a possible phytomedicine from this plant.
is needs to be confirmed through further studies.1 Abbreviations CAM: Cameroon DMSO: Dimethyl sulfoxide DPPH: 2,2-Diphenyl-1-picrylhydrazyl FRAP: Ferric ion reducing antioxidant power GAE: Gallic acid equivalent GC-MS: Gas chromatography-mass spectrometry HNC: Cameroon National Herbarium IC 50 : Concentrations (µg/mL) of extracts that scavenge 50% of DPPH free radicals or inhibit 50% of enzyme activity iu: Index unit NIST: National Institute of Standards and Technology RAS: Radical scavenging activity RSD: Response surface design-type central composite GC: Gas chromaoyography RUE: Rutin equivalent SRF: Cameroon forest reserve society TAC: Total antioxidant capacity TAE: Tannic acid equivalent TFC: Total flavonoid content TFnC: Total flavonol content TPC: Total phenolic content

Data Availability
e data of this manuscript are available on request from the corresponding author.

Conflicts of Interest
e authors declare no conflicts of interest.