Wild Mushrooms: Potential Natural Sources of Antioxidant and Anti-Quorum Sensing Bioactive Compounds for Medical Applications

Objective This study was aimed at determining the antioxidant, anti-quorum sensing, and in vitro cytotoxic activities of five wild mushroom extracts. Methods Wild mushrooms of Auricularia auricula-judae, Termitomyces umkowaani, Trametes elegans, Trametes versicolor, and Microporus xanthopus were collected from Arabuko-Sokoke and Kakamega National Forests, in Kenya. Specimens were identified and extracted using chloroform (CHL), 70% ethanol (Eth), and hot water (HW) solvents. Antioxidant and cytotoxic activities of the extracts were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Vero cell lines, respectively, while anti-quorum sensing activities were tested against Chromobacterium violaceum. All data were compared using relevant descriptive and inferential statistics at a significance level of p ≤ 0.05. Results A total of 35 wild mushrooms were collected, identified, and classified into 14 genera. Among screened mycochemicals, fatty acids, flavonoids, polyphenols, and saponins were detected at higher concentrations. The highest free radical scavenging activities of A. auricula-judae, T. umkowaani, T. elegans, and T. versicolor were observed in 70% Eth extract with the percentage values of 76.40 ± 0.12%, 68.40 ± 0.01%, 62.40 ± 0.07%, and 66.40 ± 0.04%, respectively, whereas the HW extract of Microporus xanthopus showed free radical scavenging activity at 65.90 ± 0.02%. None of the extracts, at the tested concentrations (up to 1000 µg/mL), had shown cytotoxic activity against the Vero cell line. The HW extract of T. umkowaani and the 70% Eth extract of T. versicolor showed a statistically significant difference in the inhibitory activity of violacein production against C. violaceum at the concentration of 200 µg/mL. Conclusions The antioxidant activity of wild mushrooms can help to tackle the diseases caused by free radicals. The anti-quorum sensing potential of wild mushrooms could also provide future alternatives to conventional drug therapies cost-effectively. Further detailed chemistry of the bioactive compounds and their possible mechanisms of action responsible for the observed antioxidant and anti-quorum sensing activities are needed.

Microbial cells use the signal transduction pathway known as quorum sensing (QS) to communicate with one another and within their own species.Te QS transduction pathway is started once the signaling molecules have accumulated to a certain concentration and begun to bind to a protein detector.Finally, this route activates the genes that control pathogenicity, rotting, and bioflm formation [18][19][20].One of the most efective methods to tackle microbial diseases and antibiotic resistance is the production of anti-quorum sensing agents from natural resources (fungi, bacteria, and plants) [21].
Our bodies manufacture dangerous free radicals as a result of the respiration, digestion, and metabolism process [22].Free radicals can exist as distinct molecular species with unpaired electrons.Examples of these species include reactive oxygen species, reactive nitrogen species, hydroxyl, DPPH, superoxide anion, hydrogen peroxide, nitrite, and peroxynitrite [23].Free radicals are extremely reactive and unstable substances that can damage DNA and cell membranes, obstruct the activity of key enzymes and energy production, obstruct numerous cellular processes essential for healthy body operation, and interfere with regular cell division [23,24].
To the best of our knowledge, no research has been conducted on the detection of antioxidant and anti-quorum sensing activities of wild mushroom extracts obtained from Arabuko-Sokoke and Kakamega National Reserved Forests in Kenya.Terefore, the purpose of this work was to ascertain the antioxidant and anti-quorum sensing capabilities of bioactive compounds isolated from fve indigenous Kenyan wild mushrooms.

Description of the Study Areas.
Arabuko-Sokoke and Kakamega National Forests are situated within 3 °20′S and 39 °55′E and 34 °32′0″E and 0 °10′15″S, respectively, as shown in Figure 1.Arabuko-Sokoke and Kakamega forests are located in coastal and western Kenya with an average annual rainfall of 900 mm-1,100 mm and 1200-1700 mm, respectively.Tey are rich in biodiversity and their particular importance gives them a very high conservation value [25].

Study
Design.An experimental and exploratory crosssectional design was used.An experimental (to ensure that the right kind of data can provide a clear and efective response to the research questions) and exploratory crosssectional design (evaluates both the exposures and the outcome in study participants at the same time) was used.

Sample Collection. During the months of March and
April 2018, random collections of wild mushrooms on either tree bark or other substrates (wood, soil, or leaf litter) were made.To prevent breakage and preserve moisture content, specimens were wrapped in aluminum foil and put in an ice box.Molecular and morphological methods were used to identify the specimens, together with the assistance of mycology specialists and relevant literature.Finally, samples were preserved for additional analysis after being dried in an electric drying oven for 480 h at 30 °C [26,27].

Extraction Process of Mycochemicals.
Chloroform (CHL), 70% ethanol (Eth), and hot water (HW) solvents were used for the extraction [28,29].100 g of powdered mushroom was combined with 1 L of distilled HW (heated to 60 °C for 2 h), 70% Eth (99.9%,Sigma), and 99.8% CHL (Sigma) separately in an Erlenmeyer fask at 25 °C and shaken at 150 rpm for 72 h.Whatman No. 1 flter paper was used to flter the extracts, which were then dried in a rotary evaporator at 50 °C after being centrifuged at 3000 rpm for 15 min.For additional studies, the extracts were kept in a − 80 °C deep freezer, freeze-dried, and then kept in a refrigerator at 4 °C in an amber-colored bottle.

Qualitative Mycochemical Screening Assay.
Te extracts were subjected to a qualitative mycochemical screening analysis according to standard procedures [4].Distilled water was used to dissolve all extracts.
A foam test was used to determine the presence of saponins.5 mL of distilled water and 1 mL of each 10 mg/mL extract were added, and then the mixture was shaken vigorously.Te presence of saponins is indicated by foam production.Te ferric chloride (FeCl 3 ) test revealed the presence of polyphenols.A solution of 2 mL of distilled water, 3 drops of 10% FeCl 3 , and 3 drops of potassium ferrocyanide were mixed with 1 mL of each extract at a concentration of 10 mg/mL.Blue or green color formation shows the presence of polyphenols [30].0.5 g of each mushroom extract was boiled in 10% HCl and fltered.To each 2 mL of fltered extracts, 10% ammonia solution was added.Te development of pink color in the aqueous layer is a sign that anthraquinones are present [30].
Terpenoids were detected by combining 5 mL of mushroom extracts with 2 mL of CHL, followed by a dropby-drop H 2 SO 4 along the tube wall.Te presence of terpenoids is shown by the production of brown color at the test tube interface [4].
Te tannins were determined by adding 3 mL of 10% FeCl 3 to each 3 mL of mushroom extract.Te presence of tannins is indicated by the production of blue/black color [30].
Mayer's test method was used to determine the alkaloids.Mayer's reagent (1.36 g of mercuric chloride and 5 g of potassium iodide diluted in 100 mL of distilled water) was used to treat a certain amount of the extracts, which resulted in the production of a cream-colored precipitate [4].

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Evidence-Based Complementary and Alternative Medicine H 2 SO 4 was used to determine favonoids.A fraction of the extracts were treated with concentrated H 2 SO 4 and the production of orange color was noticed [30].
Fatty acids were detected by placing 0.5 mL of extract onto flter paper.0.5 mL of extract at 20 mg/mL was placed on a flter paper.Te presence of fatty acids is indicated if the stain on the flter paper persists.A 2 mL of the extract at 20 mg/mL was dissolved in diethyl ether and evaporated to dryness.Te presence of volatile oil is indicated by a pleasant odor [4].
In a test tube, various extract concentrations (10,20,30,40,50,60, and 70 g/mL) were made, and 1.00 mL of 400 M DPPH was added.When stable absorption values were attained, the mixture was rapidly mixed and left at room temperature for 1 hour in a dark area.As negative controls, 1.00 mL of distilled water, 70% Eth, CHL, and DPPH were made without extracts.A 1.00 mL sample solution was placed in a cuvette and the color changed from purple to yellow and optical density of the solution was measured using a visible spectrophotometer at 517 nm.Diferent concentrations of ascorbic acid (10,20,30,40,50,60, and 70 µg/mL) were used as a standard antioxidant (positive control).Free radical scavenging activity (FRS) of each extract was expressed as the percentage inhibition of the free radical by the extracts, and it was calculated by using the following formula: where AS is the absorbance of the solution (extract + DPPH), AAA is the absorbance of ascorbic acid, and ADPPH is the absorbance of the DPPH solution.
Te extract concentration providing 50% inhibition (IC 50 ) was calculated from the graph of RSA percentage against extract concentration [23,31,32].Similar to the antioxidant activty of the extracts calculated above, the standandard antioxidant (ascorbic acid) was calculated as follows: (2) 2.7.In Vitro Cytotoxic Activity on Vero Cell Line.Te Vero cell line (P171) was grown in T-75 fasks using minimal media that was supplemented with 10% fetal bovine serum (FBS) and 100 g/mL streptomycin (pH 7.25).For 72 h, the T-75 fasks were incubated at 5% CO 2 and 37 °C to reach confuence.Confuent cells were extracted by trypsinization after being rinsed with phosphate bufer saline.After the suspension had been prepared (10 L of cell suspension, 80 L of saline, and 10 L of Trypan blue (0.40%) in a test tube), the number of viable cells was counted using an inverted phasecontrast microscope and the Trypan blue exclusion test on a hemocytometer slide.A 96-well plate was seeded with an aliquot of 2.0 104 cells/mL suspension and incubated in 5% CO 2 at 37 °C for 24 h.Ten, in a nutshell, 150 mL of the medium was added to the wells in rows H along with 1,000 g/ mL of extracts.From row H to row B, a serial dilution was performed.Row A served as the negative control, and the plates were incubated for 48 h at 37 °C with 5% CO 2 .Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test.Te capacity of the living cells to convert the yellow MTT dye into a purple formazan product served as a measure of the cells' growth.10 L of the MTT dye was added to the cells after 48 h, and they were then incubated for 4 h at 37 °C with 5% CO 2 .Evidence-Based Complementary and Alternative Medicine After removing the entire media from the plates, formazan crystals formed by live cells were dissolved by adding 50 L of dimethyl sulfoxide and vigorously shaking the mixture.Using a microplate reader and a reference wavelength of 720 nm, the absorbance of each well was measured.Te following formula was used to calculate cell viability percentage: where A is the optical density of the control and B is the optical density of extracts.Finally, results were expressed in inhibitory concentration (IC 50 ) values.If the IC 50 is > 90 µg/mL, the extract is classifed as noncytotoxic.If the IC 50 is between 20 and 89 µg/mL, the extract is classifed as moderately cytotoxic.If the IC 50 is <20 µg/mL, the extract is classifed as cytotoxic [33,34].

Qualitative Anti-Quorum Sensing Activities of Extracts.
Chromobacterium violaceum (CV026) was cultured in tryptic soy broth (Sigma) with shaking (180 rpm) and incubated at 30 °C for 18 h [35].Te overnight culture of CV026 was diluted with sterile distilled water and adjusted to a McFarland standard of 0.5 (1.5 × 10 8 CFU/ml) using a spectrophotometer.A 100 μL of CV026 and 20 μL of the 100 μg/mL C 6 HSL (hexanoyl homoserine lactone) solution (dissolved in acidifed ethyl acetate) were added onto a 5 ml of 1% warm molten tryptic soy agar.Ten, the solution was gently mixed and poured immediately over the surface of a solidifed tryptic soy agar plate as an overlay.Wells of 5 mm in diameter were made on each plate after the overlay was solidifed.A 10 mg/mL extract was dissolved in DMSO and sterilized using a 0.45 µm flter membrane.Of the 10 mg/mL extract, 20 μL, 10 μL, and 5 μL were serially diluted and flled to each well.
Another well was flled with 5 μL of 100 μg/mL of C 6 HSL and 45 μL of sterile tryptic soy broth as a negative control.Finally, assay plates were incubated at 30 °C for 72 h [36].Te lack of purple pigment around the bacterium was considered an indication of quorum sensing inhibition (inhibition of violacein production).A clear halo zone without bacterial growth (if any) indicates that the extracts have only antimicrobial activity but not antiquorum sensing activity.

Quantitative Anti-Quorum Sensing Activities of Extracts.
C. violaceum (20 µL) was inoculated into four Erlenmeyer fasks containing 100 mL lauryl blue (LB) broth and incubated at 30 °C for 18 h.Te fasks were supplemented with 100 µL of 100 μg/mL C 6 HSL and 20 µL, 10 µL, and 5 µL of extracts from a stock solution (10 mg/mL).Te fasks were incubated again at 30 °C with shaking (150 rpm) for 20 h.A 1.00 mL of culture was taken from each fask to the test tube and centrifuged at 8,000 rpm for 10 min to precipitate the insoluble violacein.Te supernatant was discarded, and then 1.00 mL of DMSO was added to the test tube and shaken for 30 seconds to completely solubilize the precipitated violacein.Te test tube was centrifuged at 8,000 rpm for 10 min to remove cells.Ten, 200 µL of the supernatant was added to a 96-well plate in triplicates, and absorbance was read at 585 nm.Te percentage of violacein inhibition is calculated as Simultaneously, the antibacterial efect of the extracts on the growth of the test bacteria was tested by culturing in the presence and absence of extracts and OD was read [37,38].

Statistical Analysis.
Te obtained results of each experiment were performed in triplicates and expressed as the mean ± SD values.Microsoft Excel Package was used to analyze quantitative data and draw graphs.All quantitative data were compared using relevant descriptive and inferential statistics at a signifcance level of p ≤ 0.05 [9].

Results
A total of 35 wild mushrooms were collected from Arabuko-Sokoke National Forest (ASF) (  Evidence-Based Complementary and Alternative Medicine   Te most prevalent substances in the HW and 70% Eth extracts of the majority of mushroom species were, in general, polyphenols, tannins, favonoids, and terpenoids.Diferent amounts of the mycochemical components of the wild mushrooms were discovered, and they declined in polarity from more polar to less polar solvents.Most substances are soluble in HW and solvents containing 70% Eth, with the exception of fatty acids and volatile oils.Te polyphenolic concentration of the aqueous and Eth extracts was higher than that of the CHL extracts.

FRS Activity of A. auricula-judae. Each extract of
A. auricula-judae (KF6) exhibited dose-dependent free radical scavenging (FRS) activity, as shown in Figure 2(a).70 g/mL was the concentration at which the FRS activity of the extracts in CHL (63.30 ± 0.01%), 70% Eth (76.40 ± 0.12%), and HW (60.90 ± 0.02%) peaked.40 g/mL, 50 g/mL, and 60 g/mL, respectively, were found to be the inhibitory concentration (IC 50 ) values for 70% Eth, CHL, and HW extracts.Te ascorbic acid showed the highest FRS activities despite the fact that all the extracts showed good FRS activities.

FRS Activity of M. xanthopus. All M. xanthopus (KF12)
extracts improved FRS activities as extract concentration rose as shown in Figure 2(b).Te HW extract had the highest percentage of FRS activity (65.90 ± 0.02%), followed by the CHL extract (61.30 ± 0.01%) and 70% Eth extract (49.40 ± 0.11%).However, the percentage of FRS activity of ascorbic acid (82.40 ± 0.01%) was determined to be signifcantly higher than that of all other extracts.Te IC 50 values of CHL and HW extracts were determined to be between 50 and 60 g/mL.

FRS Activity of T. elegans.
Te maximal percentage of FRS activity of T. elegans (ASF3) extracts in CHL, 70% Eth, and HW was 49.30 ± 0.03%, 62.40 ± 0.07%, and 55.90 ± 0.01%, respectively.When compared to all mushroom extracts, ascorbic acid (positive control) had the highest FRS activity as shown in Figure 2(d).Te IC 50 values for 70% Eth and HW extracts were 50 g/mL-60 g/mL and 60 g/mL-70 g/mL, respectively.However, the CHL extract did not reach the IC 50 value in the concentrations tested in this study.

FRS Activity of T. versicolor.
Te percentage of FRS activity of T. versicolor (KF10) extracts rose as the extract concentration increased from 10 g/mL to 70 g/mL Figure 2(e).In comparison to HW (47.90 ± 0.05%) and CHL (43.30 ± 0.02%) extracts, 70% Eth extract demonstrated the highest percentage of FRS activity (66.40 ± 0.04%) at a concentration of 70 g/mL.Te IC 50 value of 70% Eth was detected at concentrations ranging from 50 g/mL to 60 g/mL; however, the CHL and HW extracts did not achieve the IC 50 values at the doses examined in this investigation.

Cytotoxic Activity of T. elegans.
Te cytotoxic activity of the three extracts of T. elegans (ASF3) was detected in a very low efect against the Vero cell line as shown in Figure 3(d).Te IC 50 values of CHL, 70% Eth, and HW extracts showed within the range of 125 µg/mL-250 µg/mL, Evidence-Based Complementary and Alternative Medicine 500 µg/mL-1000 µg/mL, and 250 µg/mL-500 µg/mL, respectively.Te maximum percentage of cell viability at 15.625 µg/mL was observed in HW extract (98.65 ± 0.02%) followed by a CHL extract (91.53 ± 0.03%) and 70% Eth extract (90.68 ± 0.01%).Te pattern indicates that as the concentration of the extracts increases, the viability of the cells decreases.

Anti-Quorum Sensing Activity of the Mushroom Extracts
4.1.Qualitative Anti-Quorum Sensing Activity of the Extracts.
All three extracts screened for anti-QS activity inhibited violacein production in a concentration-dependent pattern as shown in Figure 4. Te violacein production was inhibited without any antibacterial efect of the extracts on the growth of the bacterium.Tis was proven by detecting the presence of the same viable cell counts in the extracts treated group and the control group after subculturing onto agar media.

Quantifcation of Violacein Inhibition of Chromobacterium violaceum (CV026).
Te HW extract of T. umkowaani (KF2) showed a maximum zone of inhibition (14.00 ± 0.04 mm) against the CV026 at the concentration of 200 µg/mL as shown in Table 3. Te halo zone (white zone) around the vicinity of the 8 Evidence-Based Complementary and Alternative Medicine agar wells was a good indicator of the inhibition of violacein production by the CV026.CHL extract of T. elegans (ASF3) and 70% Eth extract of T. versicolor (KF10), at a concentration of 200 mg/mL, also reduced the production of violacein 11.00 ± 0.05 mm and 11.00 ± 0.01 mm, respectively.All three extracts showed anti-QS activity at sub-MIC concentrations as shown in Table 4. Te percentage inhibitory efect on the production of violacein was reduced by the CHL extract of T. elegans (75 ± 0.01%), HW extract of T. umkowaani (80 ± 0.02%), and 70% Eth extract of T. versicolor (65 ± 0.03%) at 200 µg/mL.Te violacein production was reduced as the concentrations of extracts increased.It was observed that the HW extract of T. umkowaani and the 70% Eth extract of T. versicolor showed a statistically signifcant diference in the inhibition of violacein production against CV026 (df � 2, F � 11.08, p ≤ 0.05) at 200 µg/mL.

Discussion
All of the mushroom extracts in the current investigation signifcant FRS activities in doses ranging from 10 g/mL to 70 g/mL.On the other hand, a prior investigation found that the FRS activity of mushroom extracts was 2.11 mg/mL [39].Te existence of numerous diferent secondary metabolites and diferences in the quantity and potency of phenolic compounds are likely to be the primary causes of the inconsistent results of the free radical scavenging Evidence-Based Complementary and Alternative Medicine activities between the current report and earlier ones [40][41][42].Numerous other investigations also claimed that mushroom extracts with high phenolic component concentrations showed excellent FRS actions [43][44][45].
Te results of this study show that the FRS activities among the fve mushroom extracts varied widely.In this regard, the IC 50 values of the HW extracts of A. auriculajudae, M. xanthopus, T. umkowaani, and T. elegans were observed within the concentration range of 50 g/mL-70 g/ mL, with the exception of T. versicolor.In contrast to the results of earlier investigations, the current results' IC 50 values were observed at relatively low extract concentrations.For example, many researchers have observed IC 50 values of mushroom extracts at 8.68 mg/mL [46], 340 µg/mL [47], and 20.02-0.68 mg/mL [48].Tese signifcant diferences could be explained by the presence of reductones, such as ascorbic acid, the number of phenolic compounds, the chemical complexity, and the chemical makeup of the extracts [49].In addition, diferent species may have diferent amounts of the antioxidant components [49,50].
Te most active FRS was found in the T. versicolor 70% Eth extract.Tis may be because the extract in question contains a variety of powerful antioxidant chemicals, which may work in concert to increase the FRS activity.According to one study report, diferent solvents may have diferent efects on the quality, strength, and quantity of antioxidant components [51].Te presence of a high concentration of phenolic acids, carotenoids, tocopherols (vitamin E), and ascorbic acids has also been linked to the antioxidant properties of mushroom extracts, according to numerous research [52][53][54][55].
Signifcant cytotoxic activity against the Vero cell line was shown by all fve mushroom species' extracts.Crude extracts having IC 50 values larger than 20 g/mL are regarded as noncytotoxic, according to the American National Cancer Institute (ANCI) recommendations [56].Te IC 50 values of all fve wild mushroom extracts were found to be within the range of noncytotoxic activity.Te inhibitory concentrations (IC 50 ) of the three extracts from each wild mushroom species difered against the Vero cell line.Te discrepancies    [57].
In contrast to our fndings, a prior investigation found that water extracts had extremely low cytotoxic activity on Vero cell proliferation [58].Tis variation could be attributable to the geographical location and extraction procedures used in the current and earlier investigations.More research is needed, however, to determine the causes for the discrepancies and their mechanisms of action.
Te results demonstrate that the CHL and 70% Eth extracts have higher cytotoxic activity than the HW extract.Te existence of numerous and similar chemicals inside the extracts could explain why the CHL and 70% Eth extracts performed better.T. versicolor polysaccharides, particularly β-glucans, were found to be the most efective antitumor agents [59].Water-insoluble and alkali-soluble polysaccharides, on the other hand, were shown to have little or no anticancer activity [60,61].
Te anti-QS activity of the T. umkowaani HW extract, the T. elegans CHL extract, and the T. versicolor 70% Eth extract was qualitatively evaluated against CV026.Tere are no prior publications to compare this work with, as it is the frst to demonstrate the anti-QS efcacy of the mentioned mushroom extracts against CV026.
Te anti-QS efect of the extracts may result from their ability to limit the production of acyl-homoserine lactones (AHLs), to degrade AHLs, or to block the actions of the autoinducers, despite the fact that their mode of action has not been thoroughly explored.In addition, the CV026's QS system may be interfered with and the genes involved in the quorum sensing process may be downregulated as a result of the synergistic efects of the bioactive substances found in the extracts.According to earlier research, biological quorum quenchers typically disrupt the bacterial quorum sensing circuits by inhibiting the production, transmission, and reception of AHLs signals as well as their receptors with antagonist molecules that are structurally related to AHLs competitive inhibitors [62,63].

Conclusion
Fatty acids, favonoids, volatile oils, polyphenols, and saponins, which have potential uses in nutrition and medicine, were found in considerable concentrations among the mycochemicals that underwent qualitative screening.According to the present fndings, mushroom extracts could be good candidates for anti-quorum sensing and antioxidant agents.Te mushroom extracts demonstrated very little cytotoxic activity against the Vero cell line indicating that the mushrooms are safe for use in future downstream processes for drug development.Te synthesis of the pigment violacein in C. violaceum was signifcantly reduced as a result of the mushroom extracts' anti-quorum sensing abilities.Te development of novel alternatives for quorum sensing disruption is attracted by the extracts' ability to quench the quorum sensing activity of the bacterium.Untapped mushrooms' anti-quorum sensing capabilities may add a new dimension to research into the creation of inventive anti-quorum sensing agents that could one day ofer cost-efective alternatives to the current pharmacological regimens.Te bioactive ingredients that are responsible for the antioxidant and anti-quorum sensing actions need to be identifed through additional research.Te bioactive substances' precise chemical makeup and any potential mechanisms underlying the observed antioxidant and anti-quorum sensing properties also require a further research.

Figure 1 :
Figure 1: Map of the study areas.

Table 2 :
Qualitatively screened mycochemicals of wild mushrooms collected from Kakamega National Forest.

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Evidence-Based Complementary and Alternative Medicine and Auricularia (KF6).Te majority of the chemicals lacking from the CHL extracts of most mushroom species were present in the HW and 70% Eth extracts of all mushrooms.All mushroom species' CHL extracts lacked alkaloids or anthraquinones.Similarly, none of the mushroom species' HW and 70% Eth extracts contained any fatty acids or volatile oils.

Table 3 :
Anti-quorum sensing activity of mushroom extracts.
QS, quorum sensing.Values are expressed as mean ± SD.

Table 4 :
10rcentage inhibition of production of violacein in C. violaceum.All values are presented as percentages of the results from the control and are expressed as mean ± SD of three independent (triplicate wells) experiments.* is expressed as the signifcant diference among the three mushroom extracts (p < 0.05).10Evidence-BasedComplementary and Alternative Medicine in cytotoxic activity among mushroom extracts may be attributed in part to changes in compound polarity and solubility, bioactive component modes of action, and the presence of various cytotoxic chemicals in each extract * 54 ± 0.01 43 ± 0.02