Morphological, Biochemical, and Molecular Diversity Assessment of Egyptian Bottle Gourd Cultivars

The genetic variability and relationships between ten bottle gourd cultivars were evaluated based on morphological, biochemical, and molecular parameters. The results displayed high variability among selected cultivars in terms of photosynthetic pigments, total free amino acids, total phenol content, isozymes pattern, and protein electrophoresis. Furthermore, differences in molecular markers were revealed by the SCoT technique. The peroxidase (POD) and polyphenyl oxidase (PPO) isozymes patterns did not detect significant differences in bands among cultivars. The protein patterns revealed seventeen bands ranging from 126 to 9 kDa and five polymorphic bands representing 29.41%. On the other hand, eight SCoT primers were used to evaluate the genetic variability and relationships between the ten Egyptian bottle gourd cultivars. The results of SCoT analysis detected 44 amplicons with 50% polymorphism. In addition, the results of the phylogenetic tree that is constructed based on the similarity coefficient revealed by SCoT analysis confirm the results of biochemical analysis indicating a genetic relationship between the most efficient bottle gourd cultivars (S1 and S2 cultivars). In addition, there is a genetic relationship among the less efficient bottle gourd cultivars (S4 and S5 cultivars). These results could be beneficial to distinguish among bottle gourd cultivars in the plant breeding programs.


Introduction
Bottle gourd (Lagenaria siceraria (Mol.)Standl.)belongs to the Cucurbitaceae family.Tis crop is popularly known for the nutritional, medicinal, and economic properties of seed and edible fruits [1].Te seed is an excellent source of edible proteins, oils, vital fatty acids (such as omega 3 and 6), vitamins (A, C, and E), sterols, and antioxidants [1,2].Fresh fruit is cooked as food but mature dry fruit is utilized to make containers for grain and food storage, musical instruments, and garnishes [3].Moreover, they have medicinal usage due to the presence of pharmaceutically vital bioactive compounds that can be utilized as remedies for hirsutism, acne, alopecia, and hyperseborrhea [1,2].In Egypt, bottle gourds are planted to produce seeds that can be used as a snack or to produce salad oil [2].
Te cross-pollinating nature of this crop led to variations in fruit and seed morphology and their other traits.Seed and fruit characteristics are economic features in the development of bottle gourd cultivars that are essential for numerous industrial and domestic applications [3].
Morphological, biochemical, and molecular analysis used to detect and evaluate the phylogenetic tree among cultivars because they provide excellent information with the superior power of resolution for genetic variation analysis.Tus, morphological, biochemical, and molecular characterization is necessary to elucidate the genetic relationships among the variety of cultivars of Cucurbita [4,5].
Although there is signifcant variation in the vegetative traits of bottle gourd, particularly fruit characters, it is hard to distinguish among genotypes on their morphology alone [3,6] whereas the evaluation of genetic variation based on morphological characteristics has restrictions as the majority of phenotypes are highly afected by environmental factors and also depend on the developmental stages of the plant [3].
In contrast, isozymes are benefcial biochemical markers for the valuation of genetic diversity.Tey are utilized in taxonomic, evolutionary, ecological, and genetic research.Moreover, isozymes are utilized for the identifcation of cultivars [4].
Furthermore, SDS-PAGE (sodium dodecyl sulfatepolyacrylamide gel electrophoresis) is widely used as a rapid method to assess and identify the phylogenetic relationships between cultivars [5,7].However, diferent molecular markers based on polymorphisms are independent of environmental conditions and have proven to be forceful tools in the evaluation of genetic variability and clarifcation of the genetic relationships among and within cultivars and also reveal higher levels of polymorphisms [3,6].Te evaluation of genetic variation in bottle gourd using molecular markers allows more efcient usage of plant traits in improving cultivars suitable for high yield and stability [6].Te SCoT markers (start codon targeted) technique is the type of molecular marker that depends on polymorphism in the short-conserved region fanking the start codon ATG [8][9][10].
Te use of morphological, biochemical, and molecular markers to determine genotypic diferences among Egyptian bottle gourd cultivars is an efective strategy for genetic improvement program.Terefore, the current study aimed to identify the genetic variation among ten cultivars based on diferences at phenotypic, biochemical, and molecular levels using SDS-PAGE, isozymes, and SCOT molecular markers.

Materials and Methods
2.1.Plant Materials.Ten Egyptian bottle gourd cultivars in the S2 generation were used in this study, which were obtained from the previous research work conducted by Ibrahim [1] by using a pedigree selection program on the Egyptian bottle gourd genotypes.Seed were sown at Dakahlia, Egypt, on 3 rd May 2021.
Te experiment was conducted in a randomized complete block design (RCBD) in three replications.Each experimental unit area was composed of 2 ridges each 3.5 meters in width and 7 meters in length, and one plant per hill 50 cm away.A recommended package of practices for bottle gourd production in Egypt was followed by Prof. Dr. Ehab A. Ibrahim (frst author).
Two months after planting, a random sample of three plants was taken from each experimental unit, and observations were recorded on morphological parameters like stem length (cm), the number of leaves per plant, and the node number of the frst female fower.At the harvesting stage, a random sample of six plants was taken from each experimental unit, and observations were recorded on the number of fruits per plant, 100-seed weight (g), and seed yield per plant (g).Seeds were collected, washed, dried, and weighed.

Determination of Photosynthetic
Pigments.Pigments of photosynthesis (carotenoids, chlorophyll a, and b) in fresh leaves were determined according to Stino et al. [11].

Estimation of the Total Phenolic Content.
Te total phenolic content of the bottle gourd samples was determined using the spectrophotometric method of Folin-Ciocalteu (FC) [12] with some modifcations and was expressed as gallic acid equivalents (GAE) 100 g −1 FW of bottle gourd samples.

Determination of Total
Free Amino Acids.Te total free amino acids in the ten bottle gourd leaf samples were measured using the spectrophotometer according to the method described by Jayaraman [13].

Protein Electrophoresis.
Sodium dodecyl sulfatepolyacrylamide gel (SDS-PAGE) was used to study the protein profles of the ten bottle gourd cultivars.Watersoluble protein fractionation was performed on 12% (W/V) vertical slab gels using BIORAD Techware 1.5 mm according to the method of Laemmli [14] as modifed by Studier [15].Te molecular weight of protein bands was relatively 2 Genetics Research estimated using a wide range of molecular weights related to protein markers (Fermentas comp.).After electrophoresis, the gel was stained with Coomassie brilliant blue.

Isozyme Electrophoresis.
Extraction of peroxidase (Px) and polyphenyl oxidase (PPO) isozymes in 100 mg freshweight leaf samples were obtained by cellular lysis using an osmotic shock/freezing-thawing method described by Fernandes and Simoni [16].Native PAGE (native polyacrylamide gel electrophoresis) isozyme electrophoresis was performed on 12% (W/V) slab gels described by Davis [17] to identify isozyme diferences between ten bottle gourd cultivars.Ten, gels were stained according to Tankesley and Rick [18] for Px and PPO isozyme.Te stained gels were incubated at 37 °C in dark conditions for complete staining after adding the appropriate substrates and staining solutions.

Statistical Analysis.
Te morphological data obtained were statistically analyzed according to Snedecor and Cochran [19].Diferences among means were compared using the least signifcant diference (LSD 0.05) value 5% level.Te protein, isozyme, and SCoT banding pattern was photographed using the Gel Documentation 2000 Bio-Rad system and analyzed by GelAnalyzer3 software which scored clear amplicons as present (1) or absent (0) for each primer and entered in the form of a binary data matrix.Subsequently, the collected data were analyzed, and ten bottle gourd cultivars' coefcient of similarity was determined using Jaccard's coefcient of similarity [20] and built as a dendrogram [21] by the UPGMA method by the arithmetic average utilizing the MVSP (Multivariate Statistical Program) version 3.22 computer program.
Moreover, the potential of SCoT markers for the evaluation of genetic variation was evaluated by measuring the parameters of polymorphism information content (PIC), marker index (MI), efective multiplex ratio (EMR), and resolving power (Rp) for each primer according to [22].

Morphological Parameters of the Bottle Gourd Cultivars.
Te morphological data are presented in Table 2. Te morphological variation in fruit shape, size, and color of the ten bottle gourd cultivars studied is presented in Figure 1, and variation in seed morphology is depicted in Figure 2.
Figure 3 shows the means of the ten bottle gourd cultivars for each trait that was examined.Te obtained data show that all cultivars showed highly signifcant variations for all examined variables.Te mean performance gave an indicated the agricultural superiority of some lines over others.Results show that S2 and S9 possessed the highest values for stem length S1, S3, S7, and S8 possessed the lowest values.With regard to the number of leaves per plant, S2, S4, and S9 had maximum values, whereas S3 and S5 had the lowest values.Data revealed that S4, S6, S8, S9, and S10 had the lowest means of node number of frst female fowers compared with other families.For the number of fruits per plant, S2, S4, S6, and S9 showed profuse fruiting compared to other lines.S3 and S6 showed a maximum 100-seed weight, while S8 showed the lowest mean.For yield per plant, S1, S2, S5, and S6 exhibited the highest values, but S3, S8, S9, and S10 exhibited the lowest values compared to other cultivars.3 shows the total photosynthetic pigments content, as well as chlorophyll a, b, and carotenoids (p < 0.05) of fresh leaves of ten bottle gourd cultivars.Te signifcant diferences in photosynthetic pigments content were recorded among the ten bottle gourd cultivars.Te S2 cultivar has the greatest signifcant content of total photosynthetic pigments (1.02 mg/100 gm) followed by S1 (1.01 mg/100 gm) while the lowest content was noticed in the S5 (0.19 mg/100 gm) whereas the S2 cultivar recorded the highest signifcant Genetics Research content of chlorophyll b and carotenoids content (0.26 mg/ 100 gm and 0.46 mg/100 gm, respectively) and high content of chlorophyll a (0.30 mg/100 gm) followed by the S1 cultivars recorded the highest signifcant content of chlorophyll a content (0.39 mg/100 gm) and the high content of chlorophyll b and carotenoids (0.24 mg/100 gm and 0.38 mg/ 100 gm, respectively).Te S5 cultivars recorded the lowest signifcant content of chlorophyll b and carotenoid content (0.03 mg/100 gm and 0.06 mg/100 gm, respectively) and low content of chlorophyll a (0.10 mg/100 gm).

Estimation of Total
Free Amino Acids.Estimation of total free amino acids in leaves of ten Egyptian bottle gourd cultivars are presented in Table 3.A signifcantly higher level of total amino acid content (0.49 mg/g) was observed in the S3 cultivar than in other cultivars followed by the S2 cultivar (0.42 mg/g) and then the S1 cultivar (0.40 mg/g) whereas a signifcantly lower level of total amino acid content (0.01 mg/g) was observed in the S4 cultivar followed by the S5 and the S9 cultivars (0.05 mg/g).

Protein Electrophoresis.
Te SDS-PAGE proteins were used as a biochemical marker for the ten bottle gourd cultivars in this investigation.Te electrophoretic protein banding pattern of the studied ten bottle gourd cultivars is demonstrated in Figure 4. SDS-PAGE scans, the recorded bands, their molecular mass in kilo Daltons, and the occurrence of bands as presence (1) or absence (0),    4. Te bands' pattern reveals diferences in the bands' molecular weight and optical density between the cultivars of bottle gourds under study.Seventeen bands were revealed on the protein pattern of the studied ten bottle gourd cultivars with a polymorphism percentage of 29.41% (Table 4).Te molecular weight of the polypeptides revealed ranged from 9 to 126 kDa.Te S1, S3, S4, S5, S7, and S10 cultivars recorded the maximum number of bands (17 bands), whereas the S2 cultivar recorded the minimum number of bands (15 bands).Te resulting profle comprises twelve monomorphic bands and fve polymorphic bands.Among the fve polymorphic bands appeared four bands in all studied cultivars except one, these bands are resolved as M − and could be applied as negative biochemical markers for cultivars.Tese negative unique bands were detected in the cultivar S8 at 126 and 100 kDa, S6 at 23 kDa, and S2 at 11 kDa, whereas the band at MW 12 kDa was absent in the S2 and S9 cultivars.
Te relationships among the tested cultivars were described using reported results from protein profles.Te matrix of similarities between these cultivars was examined in each of the pair-wise groups.Te relationships of similarity based on protein analysis ranged from 1 to 0.765 (Table 5).Te lowest similarity index (0.765) was scored among S2 and S8 cultivars.Two main categories were ofered by the dendrogram, with the frst category split into two subcategories, the frst subcategory containing S1 and S2 cultivars.While the second subcategory was split into two   Table 4: Ten Egyptian bottle gourd cultivars' protein patterns were examined using SDS-PAGE, their molecular mass in kDa, the occurrence of bands as presence (1) or absence (0), polymorphism band, percentage of polymorphism, and biochemical markers.BM a , biochemical markers including either the presence (M + ) or absence (M − ) of a given band in the genotype.6 Genetics Research groups, the frst group contained S3 and S4 cultivars but the second group included S5 and S6 cultivars.Also, the second category was split into two subcategories; the frst subcategory consists of S7 and S8 cultivars, whereas the second subcategory includes S9 and S10 cultivars (Figure 5).

Analysis of Isozymes Banding Patterns. Ten cultivars of
Egyptian bottle gourd were tested for two isozymes, including peroxidase (POD) and polyphenyl oxidase (PPO) enzymes to investigate genetic diversity (Figure 6).Two isozymes appeared in fve bands (R m of 0.3, 0.6, 0.65, 0.7, and 0.75) of which one (R m of 0.6) was polymorphic representing 20%.Tis band was absent for all cultivars except S1 and S2 cultivars.Te remaining bands were all monomorphic.In each enzyme system, three isozyme banding patterns were identifed (Tables 6 and 7).Jaccard similarity coefcient was applied to construct the matrix of similarity among ten Egyptian bottle gourd cultivars based on isozyme types.Te matrix of similarities between these cultivars was tested in each of the pair-wise groups.Te relationships of similarity based on isozymes ranged from 1 to 0 (Table 8).Te cultivar S1 showed a signifcantly lower similarity (0) with all the other cultivars.Te dendrogram provided two main categories; the frst category contains the S1 cultivar.Whereas the second category was split into two subcategories, the frst subcategory consists of S2 and S3 cultivars.Te second subcategory was split into two groups, the frst group was split into two subgroups, the frst subgroup contained S4 and S5 cultivars but the second subgroup included S6 and S7 cultivars.While the second group was split into two subgroups, the frst subgroup contained S8 and S9 cultivars but the second subgroup included S10 cultivars (Figure 7).
Te correlations among the tested cultivars were described using the reported results from the combined protein-isozyme data.Te relationships of similarity ranged from 1 to 0.778 (Table 9).Te highest value of similarities was 1 among the S3, S4, S5, S7, and S10 cultivars and of them while the lowest similarity value was 0.778 between S2 and S8 cultivars.
Using arithmetic averages, dendrograms were created using the unweighted pair-group approach (UPGMA).Te dendrogram sectioned all the cultivars based on the proteinisozyme combined data (Figure 8).Te pooled dendrogram was similar to the protein dendrogram.Te dendrogram provided two main categories; the frst category was split into two subcategories, the frst subcategory containing S1 and S2 cultivars.While the second subcategory was split into two groups, the frst group contained S3 and S4 cultivars but the second group included S5 and S6 cultivars.Also, the second category was split into two subcategories, the frst subcategory consists of S7 and S8 cultivars, whereas the second subcategory includes S9 and S10 cultivars.

Molecular Analysis.
Te eight SCoT primers applied in this study showed the amplifcation results which is shown in in Table 10.Te amplifcation profles were inspected for the presence of polymorphisms between the ten tested bottle gourd cultivars, and the eight primers generated high repeatable and scorable bands (Figure 9).Te ten studied      10.Furthermore, the parameters of the genetic varieties for the investigated populations and primers were determined with the informativeness of the primers.Te values of polymorphism information content (PIC) for the SCoT markers ranged from 0 by SCoT-2 to 0.25 SCoT-11 with an average of 0.14.Te amplifcation profle was detected by the three SCoT primers SCoT-11, SCoT-1, and SCoT-9 which showed higher PIC values (0.25, 0.22, and 0.21, respectively), based on computed PIC, produced extremely informative patterns, and has more potential for further study shown in Table 10.Moreover, the highest EMR value (6.05) was obtained by SCoT-9, while the lowest value (1.38) was obtained by primer SCoT-8 with an average of 3.06.Te following RP values were approximately (3.17) according to SCoT-8 to ( 14) according to SCoT-9 by an average of 6.88.Also, MI values range from 0 according to primer SCoT-2 to 1.25 according to SCoT-9 by an average of 0.47.Each cultivar's banding patterns were distinct according to the SCoT fngerprints.Table 11 displayed the estimated genotype-specifc markers for ten cultivars of bottle gourd.Tere were six genotype-specifc markers in total.Te S2 cultivar recorded two positive unique markers of SCoT genotype-specifc markers by SCoT-9 at 460 bp and SCoT-12 at 415 bp.Moreover, one positive unique SCoT marker scored with the S8 cultivar by SCoT-1 at 835 bp and the S9 cultivar by SCoT-8 at 400 bp.Additionally, one negative unique SCoT marker scored with the S1 cultivar by SCoT-14 at 830 bp and the S3 cultivar by SCoT-12 at 500 bp.While the cultivars S4, S5, S6, S7, and S10 did not record any unique SCoT marker.Tese bands may be utilized to discriminate the tested cultivars.

Genetics Research
Te genetic similarity coefcient among ten bottle gourd cultivars ranged from the lowest similarity (0.675) between the S5 and S9 cultivars to the highest similarity (0.889) between the S5 and the S7, as shown in Table 12.
Figure 10 displays the phylogenetic tree constructed using the similarity coefcient generated from the SCoT markers.Te ten bottle gourd cultivars were split into two main categories.Te frst main category was split into two subcategories.Te frst subcategory included the S1 and the S2 cultivars, whereas the second subcategory included the S3 cultivar.Furthermore, the second main category was split

Discussion
Te highly signifcant diferences detected among the means of the ten cultivars of bottle gourd for all morphological studied traits indicated that there was a wide range of variation among the studied genotypes for all studied traits, which provides an opportunity for selecting suitable genotypes with better performance for the studied traits.Tis result also implied that these populations of bottle gourd genotypes would respond positively to selection.Similar results were obtained by Quamruzzaman et al. [23].
Achieving sustainable farming and optimal crop production requires monitoring the physiological status of bottle gourd cultivars over time.Te photosynthetic pigments content including chlorophyll a, b, and carotenoids have been frequently used to evaluate plants' health under various environmental conditions [24].Chlorophyll a and b are photosynthetic pigments responsible for the green color of plants.Carotenoids are yellow and orange pigments that take part in photosynthesis by protecting photosystems [24,25].In human nutrition, photosynthetic pigments have a rich color making products more desirable for consumption as well as various functions in human health, such as antioxidant activity, improvements in cognitive function, anticancer properties, immunomodulation activities carotenoids as pro-vitamin A activity, and prevention of degenerative diseases as well as eye and cardiovascular health [24,25].In this study, ten bottle gourd cultivars exhibited distinct photosynthetic pigments content, as well as chlorophyll a, b, and carotenoids (p < 0.05) in fresh leaves (Table 3) were used to assess the genetic diversity and plants' health.Signifcant diferences in photosynthetic pigment content were recorded among the ten bottle gourd cultivars.Te S2 cultivar recorded the highest signifcant content of total photosynthetic pigments (1.02 mg/100 gm) followed by S1 (1.01 mg/100 gm) while the lowest content was noticed in the S5 (0.19 mg/100 gm).
Te current research provides the determined amounts of total free amino acids (the primary metabolites) and total phenol content (the secondary metabolites) present in the leaves of ten Egyptian bottle gourd cultivars.Tis may provide information on the nutritional quality and its biological functions [26].
Phenolic compounds are one of the main antioxidant constituents of natural products.Data presented in Table 3 indicate that total phenolic content in bottle gourd cultivars revealed a signifcant diference (p < 0.01) with a range of 0.39 mg/100 g in the S1 cultivar to 0.11 mg/100 g in the S5 cultivar.Some studies may fnd a higher level of total phenolics than those observed in our experiments.Tese  diferences may be due to various agronomic factors such as cultivars, growing conditions, harvest time, and weather.Some researchers also demonstrated that total phenolics content strictly depends on the fruit ripening stage, as there is a higher concentration in green and intermediate ripening stages, reducing in full fruits [27].
Amino acids are a kind of nitrogenous compound that plays a vital and varied role in metabolism and have attracted large attention in feeds, foods, and nutrients.Amino acids are fundamental units and essential protein components and are involved in furthering the biosynthesis of polyamines, porphyrins, nitric oxide, glycoproteins, and neurotransmitters [28].Data presented in Table 3 indicate that total free amino acids content in bottle gourd cultivars showed a signifcant diference (p < 0.02) with a range of 0.49 mg/g in the S2 cultivar to 0.01 mg/g in the S4 cultivar.Nutrition research reveals that amino acids can alter gene expression and improve skeletal muscle growth and the small intestine.Te content of free amino acids is very signifcant in the evaluation of a protein-rich diet [28].
Biochemical markers like isozymes and proteins have evolved as a result of the restrictions of morphological markers [29].However, the genetic environment of each taxon afects how proteins attach to each other, and as a result, it is advantageous to examine the genetic diversity among cultivars [29].SDS-PAGE is an efcient tool for studying the plant genetic variety in a relatively short period.SDS-PAGE analysis can be readily used for a variety of purposes, such as biosystematics research, germplasm differentiation, varietal validation, determination of phylogenetic interactions among diferent cultivars, and the production of relevant information to balance measurement.SDS-PAGE analysis has been successful in evaluating the relationship between cultivars [29].
In this study, SDS-PAGE and two isozymes, including peroxidase (POD) and polyphenyl oxidase (PPO) enzymes were used successfully to identify relationships between ten bottle cultivars.Tese ten cultivars had higher polymorphism than the majority of the isozymes (29.41%), according to SDS-PAGE.A higher polymorphism has also been detected in other species.For instance, nine cucumber cultivars demonstrated 95.65% polymorphism, according to Habiba et al. [29].While isozyme pattern is an extremely efective genetic marker that costs less than DNA-based markers and may be detected by faster and simpler laboratory procedures.Some enzymes are more advantageous than others in variety and polymorphism analyses, according to Habiba et al. [29].For instance, in this study, peroxidase (POD) and polyphenyl oxidase (PPO) enzymes produced 5 bands of which one was polymorphic (R m of 0.6).Tese enzymes showed low suitability for diversity analysis in ten cultivars of Egyptian bottle gourd.
When describing phylogenetic relationships at the molecular level, molecular markers such as SCoT are used.SCoT is one of the molecular marker systems based on a preserved sequence of genes surrounding the start codon of translation (ATG).SCoT uses one primer constructed to anneal the ATG surrounding regions into two strands of DNA.Te SCoT technique is a dominant marker such as RAPDs.It is possible that some SCoT markers may be codominant due to deletion-insertion mutations and may be used for genetic analysis and QTL mapping [10].
In this study, the genetic markers as biochemical and molecular markers showed diferences in the level of genetic similarity among the ten Egyptian bottle gourd cultivars.Tis could be the result of the various processes of detection of polymorphism by the proteins, isozyme, and SCoT markers.Proteins and isozymes are the product of gene expression, whereas SCoT polymorphism results in diferences in the nucleotide sequence of DNA in a primer attached to random sites and the diference in DNA length between diferent primer binding sites [30].Te valuation of SCoT markers was determined in ten Egyptian bottle gourd cultivars.Te amplifcation results revealed that the cultivars by the SCoT mark assay detected good repeatability and high polymorphism.Moreover, SCoT markers have been efciently and efectively applied to detect variation between cultivars as eleven SCoTprimers were applied to estimate the genetic variety of six Egyptian soybeans [5] and ten SCoT primers were used to evaluate ten barley lines belonging to Hordeum vulgare L. [9].Similarly, Samarina et al. [10] estimated the efciency of 36 new SCoT markers for the distinction of genetic variance in chrysanthemums.
Table 10 shows the polymorphism percentages ranged between 66.66% by SCoT-8 and 0% by SCoT-2 with an average of 50%.Tis result is in harmony with the polymorphism percentages ranging between 28.57% with SCoT-22 and 66.66% with SCoT-4 [5].Abdein [4] used six SCoT primers to evaluate the genetic variance between 16 pumpkins whereas the polymorphism percentages ranged between 93.94% with SCoT-1 and 100% with SCoT8, SCoT11, SCoT12, and SCoT14.
Eight SCoT primers were investigated for their ability to detect polymorphic patterns in ten tested bottle gourd cultivars.Furthermore, to estimate the efciency of the markers, the parameters polymorphism information content (PIC), marker index (MI), efective multiplex ratio (EMR), and resolving power (Rp) were analyzed to measure genetic variation among the ten tested bottle gourd cultivars.
Tese parameters' value of the molecular marker is crucial for germplasm valuation, the building of gene banks, gene mapping, and molecular breeding.Moreover, these parameters' value is measured by the ability of the marker to create a polymorphism in the cultivars depending on the number of exposed alleles and the frequency of distribution; it is therefore equal to genetic variation [31].
Calculating the PIC of the eight selected SCoT primers' loci and the polymorphism level among the ten bottle gourd cultivars were subjected to testing allowed for the expression of their diferentiation power (DP) to measure genetic variation among these cultivars.Te maximum PIC value for dominant markers is 0.5 [10].PIC values were observed in other investigations, such as 0.38 by the RAPD marker [32] and 0.44 by the SCoT marker [5].Terefore, PIC parameters are applied to verify their benefts in fngerprint detection [33].
Table 12 shows the highest similarity value of 0.889 was scored among the S5 and the S7, while the S5 and S9 cultivars had the lowest value (0.675).Tis result is consistent with other studies that used a SCoT marker to construct the phylogenetic relationships of six Egyptian soybean cultivars, with the highest similarity value of 0.83 being scored between the Giza82 and Giza35 cultivars and the lowest value being 0.646 between the Giza82 and Giza21 cultivars [5].Te phylogenetic relationships between ten Hordeium vulgare genotypes were built by Aboulila and Mansour [34] using the SCoT marker, and they concluded that the SCoT marker is a useful tool for creating new Hordeium vulgare fngerprints.Moreover, Mohamed et al. [35] application of a SCoT marker allowed them to identify and distinguish between 14 diferent wheat cultivars, and they then divided these cultivars into numerous clusters by the dendrogram that resulted.Abdein et al. [36] used the SCoT marker on 14 summer squash genotypes.Also, Osman and Abdein [37] used molecular studies between six species of Plantago.
Based on the similarity coefcient obtained from the tested SCoT markers, the phylogenetic tree is constructed (Figure 10).Tese results confrmed the results of biochemical parameters indicating a genetic relationship between the most efcient bottle gourd cultivars (the S1 and the S2 cultivars).In addition, there is a genetic relationship among the less efcient bottle gourd cultivars (the S4 and the S5 cultivars).

Conclusion
Tis study revealed that high variance for morphological, biochemical, and molecular profles according to diferent regions.Te genetic resources description is a vital key for the management of plant breeding programs and gene banks.Te ten Egyptian bottle gourd cultivars estimated in this study showed high diversity for some fruit parameters such as fruit shape, size, and color (Figure 1), seed morphology (Figure 2) and yield traits such as the number of leaves per plant, number of fruits per plant, stem length, seeds yield per plant, the 100-seed weight, and node number of the frst female fower (Figure 3).Moreover, there is high diversity among all cultivars in the amounts of photosynthetic pigments including chlorophyll a, b, and carotenoids, with total free amino acids as primary metabolites, and total phenol content as secondary metabolites (Table 3).Furthermore, the results of protein (Figure 4 and Table 4), isozymes (Tables 6 and 7, and Figure 6), and SCoT analysis showed diferences in the degree of genetic variation among all cultivars (Table 10 and Figure 9).Te high genetic variety found could be used in plant breeding programs to identify new cultivars and to provide related information for biodiversity conservation.Te results of the phylogenetic tree that are constructed based on the similarity coefcient revealed by SCoT analysis confrm the results of biochemical parameters (Figure 5, Table 5, Figure 7, Table 8, Figure 9, and Table 9) indicating a genetic relationship between the most efcient bottle gourd cultivars (the S1 and the S2 cultivars) (Table 11).In addition, there is a genetic relationship among the less efcient bottle gourd cultivars (the S4 and the S5 cultivars) (Table 12 and Figure 10).Tese results could be benefcial to distinguish among bottle gourd cultivars in the plant breeding programs.

Figure 3 :
Figure 3: Morphological and yield traits of bottle gourd genotypes.

Figure 5 :Figure 6 :
Figure 5: Protein pattern unweighted pair-group arithmetic (UPGMA) and Jaccard's coefcient-based similarity matrices were used to create the dendrogram of the ten bottle gourd varieties.

Figure 7 :
Figure 7: Isozyme pattern unweighted pair-group arithmetic (UPGMA) and Jaccard's coefcient-based similarity matrices were used to create the dendrogram of the ten bottle gourd varieties.

Figure 8 :
Figure 8: Using pooled isozyme-protein data unweighted pair-group arithmetic (UPGMA) and similarity matrices calculated by Jaccard's coefcient; the dendrogram of the ten bottle gourd cultivars was generated.

JaccardFigure 10 :
Figure 10: Te SCoT pattern unweighted pair-group arithmetic (UPGMA) and Jaccard's coefcient-based similarity matrices were used to create the dendrogram of the ten bottle gourd varieties.

Table 1 :
Sequences of SCoT primers that were used to compare the genetic variance between the ten bottle gourd cultivars.

Table 2 :
Morphological description of fruits of bottle gourd genotypes.
L: length and D: diameter.

Table 3 :
Total chlorophyll (a, b), β-carotene, total photosynthetic pigments, total phenols contents, and total amino acids of leaves for ten bottle gourd cultivars.

Table 5 :
Te similarity matrix between ten bottle gourd cultivars as calculated by Jaccardʼs coefcient was detected with protein markers.

Table 6 :
Isozyme banding patterns of ten Egyptian bottle gourd cultivars using peroxidase enzyme.

Table 8 :
Te similarity matrix among studied ten Egyptian bottle gourd cultivars as calculated by Jaccardʼs coefcient was detected with isozyme markers.

Table 7 :
Isozyme banding patterns of ten Egyptian bottle gourd cultivars using polyphenyl oxidase enzymes.

Table 9 :
Jaccard's similarity coefcient indexes between ten Egyptian bottle gourd cultivars based on pooled isozyme-protein.

Table 11 :
Bottle gourd cultivars and their specifc SCoT markers.

Table 12 :
Te Jaccard coefcient-based similarity matrix between ten bottle gourd cultivars as detected by SCoT markers.