The Effects of Some External Management Factors on the Nitrogen Composition of Cattle Manure on Smallholder Farms

Smallholder farmers in Kenya collect manure from confined cattle housing termed zero-grazing units. Zero-grazing designs may include urine collection, though the effectiveness of these designs in improving manure N content has not been established. The manure-urine mixtures produced in these units were simulated to determine urine effects on manure N composition. Manure and manure-urine mixtures were stored for 120 days during dry and rainy seasons in Kenya. Manure-urine mixtures leached 26% of their mineral N content during the dry season, but only 12% during the rainy season. After storage, manure-urine mixtures had less organic-N and fiber-N than manure alone during the dry season (P < 0.01), but not during the rainy season. Results suggest that the effect of cattle urine on manure N composition is greater during dry seasons than rainy. Manure should not be stored more than 30 days to minimize N loss to leaching. Farmers may take steps to reduce N loss by controlling leaching and protecting manure from rainfall.


Introduction
Smallholder farmers in Kenya who confine their livestock in zero-grazing units collect and store manure for use as a soil amendment during the growing seasons [1].The design of zero-grazing units may allow for the collection and preservation of some of the cattle urine.A survey of 60 smallholders in the Kenyan highlands reported that more than half of the farms used zero-grazing units with sloped floors and manure storage piles just outside the animal confinement area at the base of the sloped floor [1].Farmers perceived that manure decomposition speeds up when manure and urine are mixed.Liquids from the zero-grazing unit floor drained into the manure storage pile.Less than 10% of farms used designs that allowed direct urine collection such as channels to transfer liquid from the zero-grazing unit floor into a reservoir just outside of the confinement area [1].The animal housing on the remaining farms had no design attributes to conserve urine.The liquid from the floor of the animal confinement area was either absorbed by bedding and feed refusals on the floor of the zero-grazing unit, or soaked into the soil [1,2].Whether the urine-conserving zero-grazing unit designs actually increased manure N content was not established.The designs may not prevent urinary N losses via volatilization [1,2].
The N retention efficiency of zero-grazing systems is poor.More than 36% of N in a mixture of manure and urine may be lost between excretion and the end of storage.Urinary-N losses are greater when no refusals to absorb the urine are on the floor of the zero-grazing unit.Manure and urine N may be lost via leaching when the dirt floors of the zero-grazing units are cleaned out only a few times each year [3].
To determine whether the presence of urine increased manure N content, experiments were conducted with manure-urine mixtures similar to those produced on farms  with zero-grazing units designed to drain urine into the manure storage piles.In addition to total manure N content, it is important to consider the patterns of transformation of nitrogenous compounds in manure that occur during storage.One objective of this study was to examine the changes in specific manure N fractions resulting from inclusion of urine in manure storage systems.It was hypothesized that most N losses from stored manure occur within the first month of storage.In a study of stored beef cattle manure, N losses of more than 10% occurred after 42 days of storage [4].A second hypothesis was that manure with urine contains more mineral N and less fiber-N and lignin-N than manure without urine.Urine is a major source of N in stored manure [5], so stored manure with urine may not be N-limited, allowing manure microbes to degrade the fibrous manure materials more rapidly.The third hypothesis was that manure mixed with urine leaches more mineral N than manure without urine.Large losses of urinary N via leaching and volatilization may occur from manure before its application to soil [6].Finally, it was hypothesized that heavy rainfall increases N losses from leaching in newly stockpiled manure because the rain saturates the manure and the water-soluble N compounds in fresh manure leach from the manure and are not recovered.The effects of season on manure composition and leaching were investigated by starting one experiment during the dry season and one during the rainy season.

Experimental Design.
Manure from two different farms in the Embu area was used to demonstrate the effects of farm and cattle diet management on manure quality.The larger farm maintained a milking herd of approximately 20 cows.The smaller farm milked 4 Holstein-Freisian cattles.The cattles on both farms were fed by the cut-and-carry method, but the cattle of the larger farm were fed energy concentrates and Napier grass (Pennisetum purpureum), a relatively high protein forage, more regularly.That the manure differed in terms of total N and organic matter (OM) content was established prior to the start of the dry season experiment to establish the effects of different management regimes on manure composition.Based on the initial manure composition and the management styles of the farms, the larger farm was designated the better-managed farm and the smaller farm the less well-managed farm.Based on this distinction in management quality, manure from the smaller farm will be referred to as "Low quality" and manure from the larger farm will be referred to as "Medium quality" from this point forward.The cattle urine used in the manure storage experiments was collected from the milking herds of both farms and mixed before being used in the experiment.The urine collection lasted approximately 10 days before the start of the dry season experiment and the start of the rainy season experiment.The urine was stored at 4 • C before the beginning of each experiment.At the start of each experiment, samples of the urine were acidified to pH < 2.5 using concentrated H 2 SO 4 to prevent NH 3 volatilization by microbes [7].Before NH 4 -N analysis, samples were stored at −20 • C. Two manure storage experiments were conducted, the first beginning in the dry season and carried out for 120 days, and the second beginning early in the long rainy season, ending after 30 days.The two experiments were designed to demonstrate the effects of rainfall on newly established manure storage systems.
The dry season manure storage experiment was conducted from January through June 2007 at the Embu office of the Kenya Agricultural Research Institute in Embu, Eastern Province, Kenya.Mid-January usually marks the beginning of the dry season in Embu, so the experiment was started on January 17, 2007.The installation of the dry season experiment marked the beginning of a 2-month period of very dry weather (Figure 1).
Five-liter plastic buckets were modified to collect leachate from the manure.The bottom of each bucket was removed and replaced with a circular piece of stainless steel 50-mesh screen with square apertures of 279 μ on a side.Each of the 120 buckets was assigned to one of four treatments and placed in one of four adjacent tables in a spatially balanced complete block design to ensure spatial homogeneity in the experiment [8].A standard funnel-graduated cylinder rain gauge was positioned at the center of the tables and rainfall was measured daily.Three replicates of each treatment were incubated.On a fresh weight (FW) basis, 4.8 kg of manure was added to each bucket.A volume of 1.4 L of urine was added to half of the buckets, representing the daily urine volume-to-fecal mass excretion ratio of cattle in water-constrained environments [9].The urine NH 4 -N concentration was 0.03 M. Three buckets from each treatment were randomly selected for destructive sampling after 6, 12, 18, 24, 30, 50, 70, 90, and 120 days of incubation.After sampling, subsamples of the manure were immediately stored at −20 • C.
The rainy season manure storage experiment, to assess the effects of rainfall on newly installed manure storage units, was installed on April 18, 2007.A preliminary period of ten days of moderate precipitation preceded the onset of the long rainy period in mid-April (Figure 1).
The goal of this experiment was to demonstrate the response of newly stockpiled manure to heavy rainfall.The design of the experiment was similar to that of the dry season experiment.Each of 60 buckets was assigned to one of the four treatments in a randomized complete block design.Three replicates of each treatment type were incubated.An initial mass of 4.1 kg (FW basis) of manure was added to each bucket.This initial manure mass differed from the mass used in the dry season experiment because the manure for this study had a greater water content.The manure for the rainy season study was collected early in the long rainy season.The rains dampened manure in the cattle pens before it was collected.An initial volume of 1.2 L of urine was added to half of the buckets, to represent the daily urine volume-to-fecal mass excretion ratio of cattle in water-constrained environments [9].The NH 4 -N concentration of the urine was 0.15 M. The higher NH 4 -N concentration of the rainy season urine likely was because cattle were fed more high-protein forages such as Napier grass during the rainy season than during the dry season.Three buckets of each treatment were sampled destructively after 6, 12, 18, 24, and 30 days of incubation.
For both the rainy and dry season experiments, leachates were collected through a plastic funnel attached to the mesh bottom of each bucket into a 60 mL bottle containing 1 mL concentrated H 2 SO 4 .The acid immediately lowered the leachate pH to less than 2.5 upon entering the bottle in order to check the degradation of organic and inorganic-N by microbes [7].The leachate collection bottles were replaced when full and upon the destructive sampling of each bucket.The mass and volume of the leachate collected were recorded.Leachates for each individual bucket were composited by date: after 6, 12, 18, 24, 30, 50, 70, 90, and 120 days of storage for the dry season experiment and after 6, 12, 18, 24, and 30 days of storage for the rainy season experiment.

Chemical Analyses.
One gram of each frozen subsample was thawed and extracted in 50 mL water for 30 min using an orbital shaker set to 180 rpm.The NH 4 -N contents of the extracts were measured using a colorimetric method [10] in the soil analysis laboratories of the Kenya Agricultural Research Institute in Embu, Kenya and of the World Agroforestry Centre Headquarters in Nairobi, Kenya.
Aliquots of the manure samples were dried at ≤60 • C and ground to pass a 1 mm screen in preparation for analysis.The samples were analyzed at the Dairy One Laboratories, Ithaca New York, USA for total N by the Kjeldahl method [11] and for neutral detergent insoluble N (NDIN) and acid detergent insoluble N (ADIN) [12].It was assumed that all mineral N volatilized from the manure during the drying process, so the measurement of total N by the Kjeldahl method was a direct measurement of organic N for the dried samples.

Statistical Analyses.
The dry and rainy experiments were designed as multi-factor models with fixed effects where the fixed factors were manure quality, urine presence, and days in storage.All possible interactions between the fixed effects were tested.The absolute concentration data were analyzed using the PROC MIXED procedure of the SAS software [13].Comparisons were made using the Student's t-test.
Using the PROC NLIN procedure of the SAS software [13], exponential decay models were developed to describe the NH 4 -N, total organic N, NDIN, and ADIN the manure in storage over time.Decay models with one exponential term were fitted to the data using the equation Y = αe kx , where Y represents the NH 4 -N, organic N, NDIN, or ADIN content of the manure, x represents the number of days the manure was incubated in storage, and k represents the rate of decay of the NH 4 -N, organic N, NDIN, or ADIN in the manure.Regression of the data using double exponential decay equations failed to converge.
The PROC NLIN procedure of the SAS software was used to develop logarithmic models describing the cumulative NH 4 -N losses from manure in storage via leaching [13].Logarithmic models with one term were fitted to the data using the equation Y = k ln(x), where Y represents the leached NH 4 -N, x represents the number of days the manure was incubated in storage, and k represents the rate of accumulation of the NH 4 -N leached from the manure.Regression of the data using log-log equations failed to converge.

Results
Results of the mixed model analyses for the dry season experiment appear in Tables 1, 2, and 3.The mixed model analyses results for the rainy season experiment appear in Tables 4 and 5.
Cattle feces contain essentially no urea [14,15].Cattle urine contains both urea and NH 4 .However, since the fecal pH was approximately pH 8, it is assumed that the urea of the urine immediately underwent hydrolysis to produce NH 4 when the urine and manure were mixed.In this study, NH 4 -N represents mineral N.
Table 1: ANOVA results for dry matter (DM) mass remaining in manure in storage in the dry season experiment, as a percent of the initial manure DM mass and for N content (DM basis) of manure in storage in the dry season experiment, as a percent of the initial manure N content.Fixed effects are the manure quality (Medium versus Low quality), the presence or absence of urine in the manure, and the number of days the manure was incubated (0, 6, 12, 18, 24, 30, 50, 70, 90, or 120 days).Terms for the interaction between fixed effects are denoted by fixed effects terms separated by an asterisk ( * ).DF represents the degrees of freedom of the test.Figure 2 shows the NH 4 -N dynamics in manure of the dry season experiments.After 30 days of storage, the NH 4 -N concentration of manure alone ranged from 156 to 241 mg NH 4 -N per kg of manure (DM basis) in individual treatments.The NH 4 -N concentration of urine-amended manure ranged from 372 to 375 mg NH 4 -N per kg of manure (DM basis) in individual treatments after 30 days of storage.The manure source had no effect on manure NH 4 -N after 30 days or 120 days (α = 0.05, Figure 2).In the dry season experiment, manure alone contained less NH 4 -N per kg of manure (DM basis) than the treatments with urine after 30 days of storage (P < 0.04).

Effect
Exponential decay models to describe the NH 4 -N dynamics of the Medium quality manure and Low quality manure in the rainy season experiment using two exponential terms did not converge.Exponential models with one exponential term to describe NH 4 -N dynamics appear in Figure 3.During the early rainy season, forages are more nutritious and digestible which may explain the marked difference in the NH 4 -N concentration of the urine used in the dry season and rainy season experiments.Neither the source of the manure nor the presence of urine in the manure affected the NH 4 -N concentration of the manure in the rainy season experiment (α = 0.05, Table 5).Average manure NH 4 -N for all treatments after 30 days of storage during the rainy season was 572 mg per kg of manure (DM basis).Figures 4(a)-4(c) show the organic N, NDIN, and ADIN dynamics in manure of the dry season experiment in terms of % remaining from the total amounts in the fresh manure at the start of the experiment.In terms of absolute content, there was no difference in the manure NDIN or manure ADIN after 30 days in storage (α = 0.05), nor was there a difference between the total organic N content of the medium and low farm manure after 30 days of storage or at Table 4: ANOVA results for N content (DM basis) of manure in storage in the rainy season experiment, as a percent of the initial manure N content, and for NH 4 -N content (mg NH 4 -N per manure storage unit) of leachate collected from manure in storage in the rainy season experiment.Fixed effects are the manure quality (Medium versus Low quality), the presence or absence of urine in the manure, and the number of days the manure was incubated (0, 6, 12, 18, 24, or 30 days).Terms for the interaction between fixed effects are denoted by fixed effects terms separated by an asterisk ( * ).DF represents the degrees of freedom of the test.the end of the experiment (α = 0.05).The days in storage had an effect on the organic N, ADIN, and NDIN contents of the dry season manure over the entire experiment (P < 0.0001, Table 1), but no treatment effects on total organic N, NDIN, or ADIN were observed after 30 days in storage (Figures 4(a)-4(c)).Single-term exponential decay models for total N, NDIN, and ADIN dynamics in manure appear in Figures 4(a)-4(c).Exponential decay models using two exponential terms to describe total N, NDIN, and ADIN dynamics in the manure failed to converge.

Effect
In general, lignin-bound N is considered to be quite refractory and poorly available for microbial degradation [16,17].Non-fiber bound nitrogenous organic compounds are more readily available for degradation than hemicellulose-bound N, cellulose-bound N, and lignin-N (represented by NDIN), and cellulose-bound N and lignin-N (represented by ADIN).The portion of the labile organic N that is microbial N can be recycled when a microbe dies and lyses and its cell contents, including nitrogenous compounds, are metabolized by other living microbes.The magnitudes of the N fractions in manure of the rainy season experiment were not affected by urine amendments (α = 0.05, Table 4).After 30 days of storage, the Medium quality manure alone contained 9.2% organic N, 4.9% NDIN, and 3.9% ADIN (DM basis).The Low quality manure contained 11.0% organic N, 5.1% NDIN, and 4.1% ADIN (DM basis, Figures 5(a)-5(c)).The source of the manure did affect N composition (P < 0.0001 for organic N, P < 0.0001 for ADIN, P < 0.0001 for NDIN) with higher levels of ADIN in the Low quality manure (P < 0.04) after 30 days of storage.The time in storage had an effect on the organic N (P < 0.008) and ADIN (P < 0.04) throughout the experiment, but at the end of the rainy season experiment, the total organic N and ADIN content of the two manures did not differ (α = 0.05, Table 4).The length of storage had no effect on NDIN (α = 0.05, Table 4).Exponential decay models with one exponential term were developed for total N, NDIN, and ADIN dynamics in manure (Figures 5(a)-5(c)).Decay models with two exponential terms did not converge.
Single-term logarithmic rate equations describing cumulative leaching of NH 4 -N from manure in the dry season experiment appear in Figure 6.Logarithmic models with two terms to describe cumulative NH 4 -N leaching failed to converge.From each of the 4 treatments, more than 50% of the final amount of NH 4 -N loss from the manure via leaching occurred during the first 30 days of storage.
Inclusion of urine significantly increased the amount of NH 4 -N leached from manure in the rainy season experiment (P < 0.0001, Table 4).Total NH 4 -N losses from urineamended manure in the rainy season experiment were larger than from urine-amended manure in the dry season experiment.Neither the manure source nor the urine treatment affected the amount of NH 4 -N leached during the 30 day storage period (α = 0.05; Figure 7).The final cumulative leaching loss from urine-amended manure storage units was larger than that from units containing only manure.After 30 days of storage during the rainy season, the urine-amended manure storage units lost an average of 337 mg NH 4  the storage units containing manure alone lost an average of 97 mg NH 4 -N.These cumulative leaching losses represent up to 12% of the initial manure NH 4 -N from urine-amended manure and up to 57% of the initial manure NH 4 -N from manure alone.After 120 days of storage in the dry season experiment, urine-amended manure lost up to 59% of its initial DM mass while manure alone lost up to 45% of the initial DM.These represent mass losses of up to 0.6 kg DM from urineamended manure and 0.5 kg DM from manure alone after 120 days of storage.At the end of the rainy season experiment, there were no treatment effects on mass loss (α = 0.05).
Figure 2 shows the NH 4 -N dynamics in manure of the dry season experiment.Exponential regression equations with one term to describe the NH 4 -N dynamics were developed using manure data for the dry season experiment (Figure 2).Attempted exponential decay models to describe NH 4 -N dynamics with two exponential terms did not converge.In the dry season experiment, the manure NH 4 -N dynamics fit an exponential decay pattern (Figure 2).Most of the NH 4 -N loss from the manure occurred during the first 50 days of incubation (Figure 2), before heavy rainfall began at 63 days of incubation (Figure 1).At the end of 120 days of storage in the dry season experiment, manure NH 4 -N fell to less than 200 mg per kg of manure, with the exception of the urine-amended Medium quality manure.In this treatment, the manure NH 4 -N concentration increased after 50 days in storage.At 120 days of storage, the NH 4 -N concentration of the Medium quality manure amended with urine was nearly 400 mg per kg of manure (Figure 2).This effect was likely due not to an increase in the pool of NH 4 -N in the manure, but the result of an increase in the manure NH 4 -N concentration as the manure decomposed.As manure decomposes, its Ccontaining compounds are converted to CO 2 and CH 4 .The loss of C mass reduces the manure C : N, and the content of manure N compounds increase [18].
In the dry season experiment, manure with urine treatments did not contain significantly more NH 4 -N at the end of the 120 day experiment than the treatments with manure alone (α = 0.05).The manure NH 4 -N concentration averaged 176 mg NH 4 -N per kg of manure (DM basis) with a range of 63 to 385 mg NH 4 -N per kg of manure (DM basis).The manure source had no effect on manure NH 4 -N after 120 days (α = 0.05, Figure 2).The presence of urine lowered the fiber-bound N content of the manure in storage.After 120 days of storage during the dry season experiment, the Medium quality manure alone contained 4.9% NDIN, and 3.9% ADIN (DM basis).Urineamended Medium quality manure contained 3.9% NDIN, and 3.2% ADIN (DM basis).At the end of the dry season experiment, the Low quality manure alone contained 6.2% NDIN, and 5.2% ADIN (DM basis).Low quality manure containing urine contained 5.0% NDIN, and 4.1% ADIN.At the end of the dry season experiment, the Low quality manure contained more NDIN and ADIN than the medium manure (P < 0.02 and P < 0.03, resp.).The average organic N content of manure after 120 days of storage was 8.6% (DM basis).Manure from both sources without urine contained more NDIN at the end of 120 days than did the urine treatments (P < 0.05).While the time in storage did have an effect on the organic N, ADIN, and NDIN contents of the dry season manure over the entire experiment (P < 0.0001, Table 1), total organic N and ADIN did not differ between the treatments after 120 days in storage (α = 0.05).
Exponential decay models with one exponential term were developed for total N, NDIN, and ADIN dynamics in manure (Figures 4(a)-4(c)).Exponential regression with two terms to describe total N, NDIN, and ADIN dynamics in manure failed to converge.Logarithmic regression using equations with two terms to describe NH 4 -N leaching losses did not converge.Cumulative leaching of NH 4 -N from manure in the dry season experiment is described by the single-term logarithmic rate equations in Figure 6.The presence of urine had a significant positive effect on the amount of NH 4 -N leached from manure in the dry season experiment (P < 0.02, Table 3).In total, the urine-amended manure units lost an average of The source of the manure, low farm or medium farm, had no effect on NH 4 -N leaching rates (α = 0.05, Table 3).

Discussion
The 14% greater loss of mass from urine-amended manure observed in this study, a loss of 0.6 kg manure DM in urineamended manure and 0.5 kg manure DM from manure alone, was a larger difference than expected.The observation supports the perception of smallholder farmers that urine speeds up the decomposition of manure in storage [1].In previous studies of composted manure, the manure lost mass over time due to the mineralization of nutrients and other degradation processes that cause structural disintegration [19].In this study, mass loss was observed.The manure continued to lose solid mass after 30 days of storage as a result of microbial activity.Farmers may find the faster degradation of urine-amended manure unacceptable because it results in a smaller volume of manure available to apply to the soil at planting.However, urine may increase the rate of decomposition of the feed refusals in manure, thereby lowering the C : N ratio of the manure and lowering the risk of N immobilization when manure is applied to soil.The results partially support the hypothesis that most N losses from stored manure occur within the first month of storage.In the dry season and rainy season experiments, International Journal of Agronomy NH 4 -N losses were large during the first 30 days of storage.In both experiments, the pools of total organic N, the more degradable hemicellulose-bound N, and the refractory fiberbound and lignin bound N had increased in content by the end of the storage period (Figures 4(a)-4(c) and 5(a)-5(c)).The increase in the recalcitrant N fractions is probably due to manure mass loss: as the manure loses mass over time, the concentration of the recalcitrant N fractions inflates [18].
Although some low molecular weight organic-N compounds may have escaped the system via the leachate, the bulk of the loss of the available N is due to the mineralization activities by microbes.The labile organic N remaining in the manure after 30 days is likely of microbial origin.The ADIN that remains, 5.1% of manure DM in the rainy experiment, likely is either part of the lignin fraction or indigestible components of microbial cell walls.The ADIN is very slowly mineralized by microbes [16,17].
The hypothesis that manure containing urine would have more NH 4 -N and less fiber-N and lignin-N than manure without urine was only partly upheld by the results of this study.In the dry season, the results were as expected.The presence of urine significantly decreased the organic N content in the manure.This effect may be due to the urine providing the microbes with a larger pool of NH 4 -N for metabolism and growth than the NH 4 -N pool in the manure solution alone.The less N-constrained environment of urine-amended manure supports larger bacterial populations than that of manure alone, so the microbial degradation of refractory manure compounds is more rapid when urine is present in the manure mixture.The results of another study of stockpiled manure on small Kenyan farms indicated that urine, when added to manure, had no effect on the nutrient composition of the manure [2].This result may have occurred because the manure piles sampled in the study differed in age or because the urinary N was lost to volatilization before being mixed with manure.The manure came from actual manure storage units on many small farms in central Kenya, and manure age was not controlled [2].
Urine may also boost the mineralization of organic N, thereby lowering the total organic N content of the manure, by increasing the water content of the manure.This effect was observed in this study, with urine amendments producing manure with less fiber-and lignin-bound N than manure with no amendments (Figures 4(b) and 4(c)).The wetter environment and increased saturation of the manure may allow microbes easier access to sites where organic N is present.Also, solubilization of OM may be facilitated in the more saturated system.Dissolved organic matter (OM) is more easily degraded by microorganisms than solid OM [20].The failure of urine to significantly affect the organic N composition of the manure in the rainy season experiment may be due to the fact that the heavy rainfall resulted in urine-amended and nonamended manure with similar water contents, so that any difference between the treatments was obscured (Figures 4(a)-4(c) and 5(a)-5(c)).
The hypothesis that the presence of urine in stored manure would leach a greater volume of leachate with a higher content of NH 4 -N than manure without urine was supported in the rainy season experiment.Urine did increase the NH 4 -N content of the leachate in both the dry season and rainy season experiments.These results suggest measures to prevent leaching losses, such as sheltering the manure from rain and creating a leachate catchment system in which the leachate is collected and returned to the manure pile, should be taken for urine-rich manure in order to conserve manure NH 4 -N.
The hypothesis that rainfall increases N losses from manure via leaching was not supported by the data for the urineamended manure.Total NH 4 -N losses from urine-amended manure in the rainy season experiment were 100 mg more (1.5 times greater) than the amount lost from urineamended manure in the dry season experiment.However, as a percentage of the initial manure NH 4 -N concentration, the urine-amended manure in the dry season lost more NH 4 -N than in the rainy season: a loss of 26% of the initial NH 4 -N in the dry season experiment versus a 12% loss of the initial NH 4 -N in the rainy season experiment.Heavy rainfall increased the volume of the leachate, and the NH 4 -N content decreased accordingly for the no urine treatments.In spite of this result, the loss of 337 mg NH 4 -N from urine-amended manure observed in the rainy experiment is an N loss that should be prevented in order to conserve manure N.

Conclusions
The results suggest the need to protect manure from rain to prevent leaching N losses.Collecting the leachate as it escapes the manure in storage and pouring it back onto the manure would be another way to conserve manure N. If manure is piled on a downhill slope immediately adjacent to fields that require fertilizing, the N-rich leachate may drain into the soil of those fields.If plastic sheeting, an affordable and readily available commodity in Kenya, is first placed on the ground and manure piled on top of it, more of the leachate would drain onto the field.Plastic sheeting could also be used to collect leachate to return to the manure.Contamination of drinking water by uncontrolled leachate runoff and seepage into soil can also be avoided by the use of plastic sheeting.A limitation of using plastic sheeting to collect leachate is the potential for large N losses from the leachate via NH 3 volatilization when the leachate is exposed to air.Covering stored manure using plastic sheeting or another material impervious to rainfall may conserve manure N by preventing leaching losses.
Manure that remains in storage for many months continues to lose mass because of microbial activity and compacts due to gravity.The result is manure with far less N than its initial content and less bulk manure to spread on the fields at planting time.To use the manure N before it is lost via leaching, it is recommended that manure not be stored more than 30 days.If the manure cannot be applied to major crops such as maize during the growing seasons, it might be applied to the kitchen gardens.These gardens often contain vegetables that provide important vitamins and minerals to the diets of farm residents.Selling manure to neighboring farms would generate income but could exacerbate the negative nutrient balances observed on most of the farms in the Kenyan highlands.

Figure 2 :
Figure 2: NH 4 -N dynamics in manure of the dry season storage experiment.Error bars represent standard error of the mean values.Continuous lines represent the best-fit regression equations for NH 4 -N dynamics in manure during the dry season experiment.Y represents the amount of NH 4 -N (mg/kg manure DM) in the manure.The solid line (-) represents the Medium quality manure, no urine treatment.The dashed line (----) represents the urine-amended Medium quality manure treatment.The dotted line (• • • •) represents the Low quality manure, no urine treatment.The dot-dash line (-••-••) represents the urine-amended Low quality manure treatment.Medium quality manure, no urine: Y = 462.3e−0.0180x , R 2 = 0.807.Medium quality manure, with urine: Y = 621.8e−0.0232x , R 2 = 0.619.Low quality manure, no urine: Y = 249.1e−0.00955x , R 2 = 0.715.Low quality manure, with urine: Y = 1033.7e−0.0570x , R 2 = 0.812.The rate equations for NH 4 -N dynamics between the manure sources did not differ at significance level α = 0.05.

Figure 3 :
Figure 3: NH 4 -N dynamics in manure of the rainy season storage experiment.Error bars represent standard error of the mean values.Continuous lines represent the best-fit regression equations for NH 4 -N dynamics in manure during the dry season experiment.The solid line (-) represents the best-fit regression for the Medium quality manure treatments.The dashed line (----) represents the best-fit regression for the Low quality manure treatments.Y represents the amount of NH 4 -N (mg/kg manure DM) in the manure.Medium quality manure: Y = 144.7e 0.0349x , R 2 = 0.508.Low quality manure: Y = 240.7e 0.0201x , R 2 = 0.571.The rate equations for NH 4 -N dynamics between the manure sources did not differ at significance level α = 0.05.

Figure 6 :
Figure 6: Cumulative NH 4 -N (mg NH 4 -N per manure storage unit) leached from manure of the dry season storage experiment.Error bars represent standard error of the mean values.Continuous lines represent best-fit regression equations for cumulative NH 4 -N (mg) leached from manure during the dry season experiment.The solid line (-) represents the Medium quality manure, no urine treatment.The dashed line (----) represents the urineamended Medium quality manure treatment.The dotted line (• • • •) represents the Low quality manure, no urine treatment.The dot-dash line (-••-••) represents the urine-amended Low quality manure treatment.Y represents the cumulative NH 4 -N (mg) leached from the manure.Medium quality manure, no urine: Y = 18.3144 ln(x), R 2 = 0.989.Medium quality manure, with urine: Y = 41.1785ln(x), R 2 = 0.993.Low quality manure, no urine: Y = 7.1964 ln(x), R 2 = 0.988.Low quality manure, with urine: Y = 30.5345ln(x), R 2 = 0.994.The rate equations for leachate NH 4 -N between the four treatments did not differ at significance level α = 0.05.

Figure 7 :
Figure 7: Cumulative NH 4 -N (mg NH 4 -N per manure storage unit) leached from manure of the rainy season storage experiment.Error bars represent standard error of the mean values.The continuous line represents best-fit regression equations for cumulative NH 4 -N (mg) leached from manure during the dry season experiment.X represents the number of days in storage.The solid line represents all treatments.Y represents the cumulative NH 4 -N (mg) leached from the manure.All treatments: Y = 70.7127ln(x), R 2 = 0.998.
Figure 1: Rainfall at the experiment site, Kenya Agricultural Research Institute.Dry season experiment duration: 17 January to 01 June 2007.Rainy season experiment duration: 18 April to 18 May 2007.

Table 2 :
ANOVA results for NH 4 -N concentration (mg NH 4 -N/kg manure, DM basis) of manure in storage in the dry season experiment.Fixed effects are the manure quality (Medium versus Low quality), the presence or absence of urine in the manure, and the number of days the manure was incubated (0, 6, 12, 18, 24, 30, 50, 70, 90, or 120 days).Terms for the interaction between fixed effects are denoted by fixed effects terms separated by an asterisk ( * ).DF represents the degrees of freedom of the test.

Table 3 :
ANOVA results for NH 4 -N content (mg NH 4 -N per manure storage unit) of leachate collected from manure in storage in the dry season experiment.Fixed effects are the manure quality (Medium versus Low quality), the presence or absence of urine in the manure, and the number of days the manure was incubated (0, 6, 12, 18, 24, 30, 50, 70, 90, or 120 days).Terms for the interaction between fixed effects are denoted by fixed effects terms separated by an asterisk ( * ).DF represents the degrees of freedom of the test.

Table 5 :
ANOVA results for NH 4 -N concentration (mg NH 4 -N/kg manure, DM basis) of manure in storage in the rainy season experiment.Fixed effects are the manure quality (Medium versus Low quality), the presence or absence of urine in the manure, and the number of days the manure was incubated (0, 6, 12, 18, 24, or 30 days).Terms for the interaction between fixed effects are denoted by fixed effects terms separated by an asterisk ( * ).DF represents the degrees of freedom of the test.