Weed Control and Peanut Tolerance with Ethalfluralin-Based Herbicide Systems

Field studies were conducted from 2007 through 2009 to determine weed efficacy and peanut (Arachis hypogaea L.) response to herbicide systems that included ethalfluralin applied preplant incorporated. Control of devil’s claw (Proboscidea louisianica (Mill.) Thellung), yellow nutsedge (Cyperus esculentus L.), Palmer amaranth (Amaranthus palmeri S. Wats.), and puncturevine (Tribulus terrestris L.) was most consistent with ethalfluralin followed by either imazapic or imazethapyr applied postemergence. Peanut stunting was 19% when paraquat alone was applied early-postemergence. Stunting increased to greater than 30% when ethalfluralin applied preplant incorporated was followed by S-metolachlor applied preemergence and paraquat applied earlypostemergence. Stunting (7%) was also observed when ethalfluralin was followed by flumioxazin plus S-metolachlor applied preemergence with lactofen applied mid-postemergence. Ethalfluralin followed by paraquat applied early-postemergence reduced peanut yield when compared to the nontreated check. Ethalfluralin applied preplant incorporated followed by imazapic applied mid-postemergence provided the greatest yield (6220 kg/ha). None of the herbicide treatments reduced peanut grade (sound mature kernels plus sound splits) when compared with the nontreated check.


Introduction
Peanut has several unique features that contribute to challenging weed management.Peanut cultivars grown in the United States require a fairly long growing season (140 to 160 d), depending on cultivar and geographical region [1,2].Consequently, soil-applied herbicides may not provide season-long control, and mid-to-late season weed pressure may occur.Peanut has a prostrate growth habit, a relatively shallow canopy, and is slow to shade interrows allowing weeds to be more competitive [2,3].Additionally, peanut fruit develops underground on pegs originating from branches that grow along the soil surface.This prostrate growth habit and pattern of fruit development restricts cultivation to an early-season control option [2,4].With conventional row spacing (91 to 102 cm), complete ground cover may not be attained until 8 to 10 wk after planting.In some areas of the U.S. peanut growing region, complete canopy closure may never occur.
Weeds compete with peanut for sunlight, moisture, and nutrients and may reduce harvesting efficiency.Weeds are particularly troublesome during digging and inverting procedures [5].Weed biomass slows field-drying of peanut vines and pods and increases the likelihood of exposure to rainfall, which may increase harvesting losses [2,5].The fibrous root system of annual grasses is extremely difficult to separate from peanut [6].
The dinitroaniline herbicides are registered for use in over forty crops [7].These herbicides provide excellent control of annual grasses [2,8,9] and are the only soil-applied herbicides registered for use in peanut that will provide full-season control of Texas panicum (Panicum texanum L.) [2,10,11].Peanut tolerance to the dinitroaniline herbicides has been questioned previously [12][13][14].Greenhouse studies showed that ethalfluralin inhibited seedling growth more than pendimethalin at equivalent rates applied preplant incorporated; however, injury by these herbicides following International Journal of Agronomy preemergence (PRE) applications were similar [15].In runner peanuts, which are more prone to peg injury compared to Spanish peanut, proper herbicide incorporation was needed to prevent injury.Merkle [16] stated that sporadic injury to runner peanut from trifluralin was due to the failure to properly incorporate the herbicide.No differences were observed in a study examining peanut growth, yield, and grade effects with ethalfluralin, pendimethalin, or trifluralin in two different studies [13,17].In Florida, ethalfluralin did not cause peanut injury at any rate or application timing [12].Dinitroaniline injury on peanut includes a swollen hypocotyl, abnormal lateral root growth, and stunted plants [15,16].
Metolachlor is commonly used in peanut for control of small-seeded broadleaf weeds, some annual grasses, and yellow nutsedge [18].S-metolachlor is labeled in peanut for either preplant incorporated (PPI), post-plant incorporated, PRE, postemergence (POST), or at lay-by [19].The Smetolachlor registered rate for the southwest is 1.1 to 1.4 kg/ha [19].However, many growers have reported peanut stunting when PPI or PRE applications of metolachlor have been followed by large amounts of rain [18].Grichar and Colburn [18] reported that POST applications of metolachlor followed by (fb) irrigation within 24 h could be effective for yellow nutsedge control and reduce the chance of peanut injury from soil applications.Combinations of factors, such as herbicide rate, moisture conditions at planting, soil organic matter, and pH may affect peanut injury by chloroacetamide herbicides such as S-metolachlor [20][21][22][23].Cardina and Swann [20] reported that metolachlor often delayed peanut emergence and reduced peanut growth when irrigation followed planting.However, yield loss was observed only when metolachlor was applied at three times the manufacturer's suggested use rate.
Imazethapyr applied POST provided broad spectrum and most consistent control when applied within 10 d of weed emergence [24,27].Imazethapyr and imazapic are the only POST herbicides to effectively control both yellow and purple nutsedge [26,28].Control is most effective when imazethapyr is applied to the soil or to yellow nutsedge that is no more than 13 cm tall [2,27,28].
Peanut tolerance to paraquat was first noted in the late 1970s [2].Wehtje et al. [21] reported that paraquat controlled Texas panicum when applied sequentially, and Johnson III et al. [32] noted differences in cultivar tolerance.Paraquat is often tank-mixed with bentazon and can be applied up to 28 d after peanut emergence [2,33].Bentazon increases control of paraquat-tolerant species such as bristly starbur (Acanthospermum hispidum DC.), coffee senna, prickly sida, and smallflower morninglory [Jacquemontia tamnifolia (L.) Griseb.][34] and reduces paraquatinduced injury to peanut [34].Paraquat is a standard herbicide in southeastern U.S. peanut weed management [34,35].However, paraquat lacks residual activity and has a narrow window of application [2].
Lactofen was registered for POST application for peanut in 2005 for control of several annual broadleaf weeds including annual morningglories [36].Peanut and soybean (Glycine max L.) tolerance to lactofen is based on metabolism, which often results in some leaf bronzing and spotting of leaves and plant growth is often temporarily reduced [37].
Flumioxazin is a soil-applied herbicide that received a federal label in the U.S. for use in peanut in 2001 [38].In Georgia, flumioxazin applied PRE was shown to control morninglory spp., prickly sida, and Florida beggarweed [39] while in Texas pitted morninglory [Ipomoea lacunose L.] was controlled greater than 75% with PRE applications of flumioxazin [17].
The herbicide 2,4-DB has provided inexpensive broadleaf weed control in peanut for many years [2,8,40].Visual injury symptoms of 2,4-DB can be observed in the form of rolled or elongated foliage [41,42].
Growers in the southwest consider ethalfluralin to be the premier dinitroaniline herbicide used in peanut production.The objectives of this research were to evaluate weed efficacy and/or peanut crop tolerance to soil-applied applications of ethalfluralin fb, PRE, and POST applications of commonly used peanut herbicides.under noncropland conditions to determine weed control with ethalfluralin applied PPI followed by PRE and/or early postemergence (EPOST) or mid-postemergence (MPOST) treatments of several commonly used peanut herbicides.Peanut tolerance was also evaluated at the south Texas location.The south Texas study was located in the same general area and with the same producer but in different fields near Pearsall on a Duval loamy fine sand (fine-loamy, mixed, hyperthermic aridic Haplustalfs) with less than 1% organic matter and pH 6.9 to 7.2.The High Plains location was at the Texas AgriLife Research and Extension Center near Lubbock on an Acuff loam (fine-loamy, mixed, thermic aridic Paleustoll) with 0.8% organic matter and pH 7.8.

Materials and Methods
The peanut cultivar Tamrun OL02 [43] was planted at the south Texas location using a seeding rate of 90 to 110 kg/ha in all years.Each plot consisted of two rows spaced 97 cm apart and 7.6 m long, while in the High Plains location, plots were 4.1 m wide by 9.1 m long.At the south Texas location, sprinkler irrigation was applied on a 2-to 3-wk schedule throughout the growing season as deemed necessary by the grower.All field plots were naturally infested with moderate to high populations of yellow nutsedge ( 6 The experiment was conducted as a randomized complete block design with three replications.An nontreated check was included in each experiment.Herbicide treatments included ethalfluralin alone at 0.84 kg ai/ha applied PPI, ethalfluralin fb imazethapyr at 0.07 kg ai/ha applied MPOST, ethalfluralin fb imazapic at 0.07 kg ai/ha applied MPOST, ethalfluralin fb imazapic at 0.035 kg ai/ha applied EPOST fb imazapic at 0.035 kg/ha applied MPOST, ethalfluralin fb bentazon at 1.12 kg ai/ha applied MPOST, ethalfluralin fb acifluorfen at 0.42 kg ai/ha applied MPOST, ethalfluralin fb lactofen at 0.22 kg ai/ha applied MPOST, ethalfluralin fb 2,4-DB at 0.44 kg ai/ha applied MPOST, ethalfluralin fb paraquat at 0.28 kg ai/ha applied EPOST, ethalfluralin fb S-metolachlor at 1.1 kg ai/ha applied PRE fb paraquat at 0.28 kg/ha applied EPOST, ethalfluralin fb flumioxazin at 0.11 kg ai/ha applied PRE, ethalfluralin fb flumioxazin at 0.11 kg/ha plus S-metolachlor at 1.1 kg/ha applied PRE, ethalfluralin fb S-metolachlor at 1.1 kg/ha applied PRE fb lactofen at 0.22 kg/ha applied MPOST, and ethalfluralin fb flumioxazin at 0.11 kg/ha plus S-metolachlor at 1.1 kg/ha applied PRE fb lactofen at 0.22 kg/ha applied MPOST.Where peanut were planted, PRE herbicide applications were made immediately after planting in 2007 and 2009 and 3 d after planting in 2008.Early-postemergence applications were made approximately 3 to 4 wk after planting when Palmer amaranth was approximately 10 to 15 cm tall, while MPOST applications were made approximately 6 to 7 wk after planting when Palmer amaranth was 20 to 30 cm tall.All POST herbicide applications included either a crop oil concentrate (Agri-Dex, a blend of 83% paraffin-based petroleum oil and 17% surfactant; Helena Chemical Company, Suite 500, 6075 Poplar Avenue, Memphis, TN 38137) at 2.3 L/ha or a nonionic surfactant (X-77, 90% non-ionic surfactant; Loveland Industries, P.O.Box 1289, Greeley, CO 80632) at 0.25% v/v.
At the south Texas location ethalfluralin was applied with a tractor-mounted sprayer equipped with Teejet 11002 DG flat fan spray tips (Spraying Systems Company, P.O.Box 7900, North Avenue, Wheaton, IL 60188).A rolling cultivator with mixing wheels was used to incorporate ethalfluralin immediately after application.The mixing wheels consisted of four to six spider gangs approximately 10 to 13 cm long mounted on a gang tube which were set to incorporate the herbicide no greater than 2 cm.These gangs help to lift and mix the soil.Gang angle was set to maintain the shape of the raised beds.All PRE and POST herbicides were applied with a CO 2 pressurized backpack sprayer equipped with Teejet 11002 DG which delivered a spray volume of 190 L ha −1 at 180 kPa.At the High Plains location, PPI and PRE herbicides were applied with a tractor-mounted sprayer using Teejet 110015 TT flat fan nozzles.In both years, both PPI and PRE herbicides were sprayed and incorporated (2 to 3 cm) with a rolling cultivator.All POST herbicides were applied with a CO 2 pressurized backpack sprayer using Teejet 110015 TT flat fan nozzles calibrated to deliver a spray volume of 95 L ha −1 at 207 kPa.
Weed control and peanut injury (expressed as stunting) were estimated visually on a scale of 0 to 100 with 0 indicating no weed control or peanut stunting to 100 indicating complete weed control or peanut stunting (plant death), relative to the nontreated check [44].At the High Plains location, weed-control ratings were taken approximately 90 days after ethalfluralin was applied while at the south Texas location weed control and peanut stunting evaluations recorded prior to digging of peanut are presented.Peanut yields were obtained by digging each plot separately, air-drying in the field for 4 to 7 d, and harvesting peanut pods from each plot with a combine.Weights were recorded after soil and trash were removed from plot samples.Grade samples were determined using screens specified in USDA grading procedures [45].

Statistical Analysis.
Peanut injury data were transformed to the arcsine square root prior to analysis of variance but are expressed in their original form for clarity because the transformation did not alter interpretation.Visual estimates of peanut injury, yield, and grade were subjected to analysis of variance to test effects of POST herbicide and application timing.Means were compared with the appropriate Fisher's Protected LSD test at the 5% probability level.The nontreated check was not included in peanut stunting analysis but was included in peanut yield and grade analysis.International Journal of Agronomy 3.1.Devil's-Claw Control.In 2008, ethalfluralin alone controlled devil's-claw 20% while herbicide systems which included either imazethapyr or imazapic provided at least 90% control of this species (Table 1).No other herbicide systems with the exception of ethalfluralin fb bentazon or 2,4-DB controlled devil's-claw better than 75%.In 2009, herbicide systems which included either imazethapyr, 2,4-DB, paraquat alone, or flumioxazin alone failed to control devil's-claw.Herbicide systems which included imazapic, bentazon, lactofen, S-metolachlor fb paraquat, or lactofen controlled devil's-claw at least 90%.Differences in control may be partially explained by differences in weather conditions between the two years.In 2008, extremely dry conditions existed from herbicide application through the first 6-to 8-wk period with only approximately 3 mm of rainfall.In comparison, rainfall in 2009 for the same time period was approximately 32 mm.Also, temperatures were much warmer in 2009 than 2008 during this time period (data not shown).In earlier work, Grichar et al. [46] reported that ethalfluralin alone did not control devil's-claw; however, ethalfluralin fb imazapic applied POST provided 100% control.In commercial fields, imazapic has provided excellent devil's-claw control (author's personal observations).

Palmer Amaranth Control.
At the High Plains location in 2008, ethalfluralin fb imazapic, ethalfluralin fb Smetolachlor applied PRE fb paraquat applied EPOST, and ethalfluralin fb flumioxazin plus S-metolachlor with or without lactofen applied MPOST controlled Palmer amaranth at least 80% (Table 1).Ethalfluralin fb either bentazon, acifluorfen, or paraquat failed to control Palmer amaranth (≤60%).In 2009, all herbicide systems controlled Palmer amaranth at least 90% with the exception of ethalfluralin fb bentazon which controlled this weed 87%.At the south Texas location in 2007, all herbicide systems with the exception of ethalfluralin alone, ethalfluralin fb imazethapyr or bentazon control Palmer amaranth at least 88%, while in 2008, all herbicide systems controlled Palmer amaranth at least 91% (Table 2).Increased herbicide efficacy with herbicide systems in 2008 was due to the lack of high Palmer amaranth populations that were observed in 2007.

Yellow Nutsedge Control.
Herbicide systems that included imazethapyr or imazapic controlled yellow nutsedge at least 83%, while herbicide systems which included Smetolachlor, controlled yellow nutsedge no better than 75% (Table 2).Lack of yellow nutsedge control with flumioxazin in other studies [18,39] suggest the need for a chloroacetamide herbicide when this weed is present.Askew et al. [49] reported that metolachlor PRE complemented a flumioxazin-based program by controlling goosegrass [Eleusine indica (L.) Gaertn.] and yellow nutsedge.Scott et al. [50] reported that metolachlor alone applied PPI or metolachlor followed by flumioxazin PRE controlled at least 86% yellow nutsedge.However, the addition of S-metolachlor to flumioxazin systems provided inconsistent yellow nutsedge control in this study.Bentazon will suppress yellow nutsedge growth, but control may be incomplete due to the lack of sufficient translocation in the plant [27].Imazethapyr applied PRE controls yellow nutsedge, but control can be inconsistent [25,26].Grichar and Nester [47] found that imazapic at 50 and 70 g/ha controlled yellow nutsedge 88% in the early season but was inconsistent later in the season.Wilcut et al. [27] reported that imazapic controlled yellow nutsedge greater than 90% at rates as low as 40 g/ha.Grichar and Sestak [51] also reported that imazapic provided at least 80% control of yellow nutsedge.

Peanut Stunt.
Herbicide systems that included paraquat resulted in severe peanut stunting, while the herbicide system which included flumioxazin plus S-metolachlor fb lactofen, caused moderate peanut stunting (Table 2).No other herbicide system resulted in significant stunting, although paraquat is registered for use on peanut within 28 d after emergence, Johnson III et al. [32] stated that paraquat use later than 28 d after emergence is discouraged due to the potential for crop injury with less time for plant recovery.Although paraquat was applied within the time frame regarded as safe to the crop, excessive stunting was still observed.Peanut cultivar response to paraquat was thought to be the issue; however, Wehtje et al. [52] reported that foliar absorption and translocation of paraquat did not vary between peanut cultivars, and they attributed yield difference to the difference in yield potential between cultivars.
Peanut stunting with metolachlor has been noted in other studies [20,21,38,53].In Georgia, early-season peanut growth suppression was related to metolachlor rate [20].Later-developing pods were not affected by metolachlor, and over the long period of pod development, the effect of metolachlor on early-developing pods was masked by the greater contribution of later developing pods to the final yield.Grichar et al. [38] reported that flumioxazin plus metolachlor combinations, under cool, wet conditions, resulted in peanut stunting which was evident throughout the growing season.They attributed this to increased uptake of flumioxazin and metolachlor with the heavy rainfall and the slowed metabolism of these herbicides as a result of cool temperatures [38].
3.6.Peanut Yield and Grade.High weed densities in plots with poor control can result in the tight fibrous root system of the weed becoming intertwined with the peanut plant and digging equipment during the digging opearation.As a result, many peanut pods can be stripped from the peanut plant during digging.Peanut pods that become detached from the plant remain unharvested in or on the soil [8].
Plots treated with ethalfluralin fb imazapic at 0.07 kg/ha produced the highest peanut yield, while herbicide systems which included acifluorfen, 2,4-DB, paraquat, or flumioxazin alone produced the least yield (Table 2).Other studies have not reported any yield reductions with the use of paraquat [32,52,54].Peanut yields do not always reflect the levels of nutsedge control, especially if annual grasses and broadleaf weeds are controlled [29,55].Also, Patterson et al. [56] and Johnson III and Mullinix [57] reported that yellow nutsedge was not as competitive with many crops, such as cotton (Gossypium hirsutum L.), as well as many annual weeds.However, yellow nutsedge needs to be controlled, since nutsedge tubers can contaminate peanut and confectionary products, increasing food industry liability [58].
Peanut grade (sound mature kernels plus sound splits) was not reduced from the nontreated check with any herbicide systems (Table 2).Carley et al. [54] reported that paraquat reduced the percentage of extra large kernels (ELK) from 45% (nontreated peanut) to 41% in one year but did not affect percentage ELK in another year on Virginia market type cultivars.Previous research suggests that paraquat may affect peanut grade (especially Virginia market types) by increasing the proportion of other kernels [59], which may indicate that the herbicide affected grade by delaying maturity [54].

Conclusions
Results from this research demonstrate that ethalfluralin fb POST applications of imazethapyr and imazapic were the most effective herbicide systems for control of all the weeds that may be present in a peanut field in Texas.Although some growers in the southwestern United States peanut production region are of the opinion that a total POST program using imazapic or imazethapyr is sufficient, the use of a soil-applied International Journal of Agronomy herbicide is important in order to maintain season-long weed control and increase net returns [13,18,46].Care must be taken to apply paraquat within the time frame regarded as safe to the crop since severe peanut stunting and yield reductions are possible [2].Monoculture production systems and the repeated use of the same or similar herbicides have led to herbicide resistance in weeds [60][61][62].Amaranthus species are very sensitive to ALS-inhibiting herbicides and possess characteristics, such as high genetic variability, prolific seed production, and efficient pollen and seed distribution, that predispose them to have herbicide resistant biotypes [63].The use of soilapplied and POST herbicides with alternative sites of action is necessary to reduce the rate of development of herbicideresistant weed populations [64].

2. 1 .
Field Studies.Studies were conducted in south Texas in a peanut grower's field near Pearsall from 2007 through 2009 and in the High Plains area near Lubbock in 2008 and 2009 to 8 plants/m 2 ), Palmer amaranth (in south Texas in 2007, 4 to 6 plants/m 2 , in 2008, 1 to 2 plants/m 2 , 12 to 15 plants/m 2 in the High Plains), devil's claw (1 to 4 plants/m 2 ), and puncturevine (1 to 2 plants/m 2 ).
At the High Plains location, under non-cropland conditions, devil's-claw and Palmer amaranth were present both years while puncturevine was present only in 2009.Devil's-claw and Palmer amaranth data were not combined over years due to a treatment by year interaction.At the south Texas location, yellow nutsedge was present in 2008 and 2009 while Palmer amaranth was present in 2007 and 2008.Yellow nutsedge data were combined over years since there was not a treatment by year interaction; however, Palmer amaranth data are presented separately by years due to a treatment by year interaction.Peanut stunt, yield, and grade data were collected in 2008 and 2009 and combined over years due to a lack of treatment by year interaction.

Table 1 :
Weed control in the Texas High Plains with herbicide systems which include ethalfluralin.a Split application of imazapic.Second application followed the initial application by 6 wk in 2008 and 2 wk in 2009.